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Pierce™ Protein A Plus Agarose Thermo Scientific™

The Thermo Scientific™ NAb™ Protein A Plus Spin Kits are convenient for rapid, small-scale affinity purification of antibodies from a variety of sample types. Each pre-filled microcentrifuge spin column of the immobilized protein resin enables quick purification of ≥34 mg of human IgG from 0.5-2 mL of serum or other sample. Included in each kit are buffers and a streamlined protocol for purifying at least two antibody samples.

Pierce Protein A Plus Agarose is a versatile, high-performance affinity resin for antibody purification. It consists of purified native Protein A that has been covalently immobilized at high density onto high-quality crosslinked 6% beaded agarose (CL-6B). Among the many available varieties of immobilized Protein A affinity resins, this one provides the most versatile combination of chromatographic features for high yield and high purity purification of whole IgG from mammalian serum samples. The agarose beads have physical and chemical properties suitable for many affinity purification systems.

Features of Pierce Protein A Plus Agarose:

Native Protein A – immobilized Protein A is ideal for polyclonal IgG purification from human, rabbit, pig, dog or cat serum
Agarose resin – support is crosslinked 6% beaded agarose (CL-6B), the most popular resin for protein affinity purification methods
Inert and stable – superior manufacturing method immobilizes Protein A by charge-free, leach-resistant covalent bonds, resulting in low nonspecific binding and enabling multiple uses without decline in yield
High capacity – this “Plus” variety of Pierce Protein A Agarose has a dense load of immobilized Protein A, providing a binding capacity greater than 34 mg human IgG/mL resin (approx. 16 to 17 mg mouse IgG/mL resin)

Protein A is a cell wall component produced by several strains of Staphylococcus aureus. The 46.7kDa-protein consists of a single polypeptide chain that is essentially devoid of carbohydrate. Native Protein A has contains four high-affinity (Ka = 10^8/mol) binding sites that are capable of interacting with the Fc region of IgG-class antibodies from selected mammalian species. IgG-binding function is optimal at pH 8.2, but efficient binding also occurs in neutral and physiological buffers (pH 7.0 to 7.6).

Protein A is a bacteria-derived protein that binds with high affinity and specificity to the Fc portion of antibodies, especially those of the IgG class. Compared to its alternative (Protein G), Protein A provides the highest binding capacity for subclasses of IgG from rabbits, pigs, dogs and cats. Protein A also has higher binding capacity for human IgG, except for IgG3, which it binds weakly. Protein A binds weakly to mouse IgG1 and is not recommended for most applications with that antibody isotype.

Pierce Protein A Plus Agarose is prepared using Thermo Scientific AminoLink Coupling Chemistry, which provides several advantages compared to traditional methods of ligand immobilization. AminoLink Immobilization results in conjugation between sugar monomers of the agarose beads and native lysine residues on the Protein A via simple amide bonds. Unlike typical cyanogen bromide (CNBr) immobilization, the AminoLink Method does not introduce any novel chemical groups that could cause undesired nonspecific binding and produces a stable, essentially irreversible bond. The result is a high-binding-capacity resin that retains functional immobilized Protein A through multiple rounds of antibody purification.

Properties of crosslinked 6% beaded agarose (CL-6B):
• Support pH Stability: 2 to 14 (short term); 3 to 13 (long term)
• Average Particle Size: 45 to 165 microns
• Exclusion Limit: 10,000 to 4,000,000 daltons
• Maximum Volumetric Flow Rate: approx. 1 mL/minute (for 1 cm diameter column)
• Maximum Linear Velocity: 30 cm per hour
• Maximum Pressure: less than 25psi (1.5 bar), defined as the maximum pressure drop across a column that the resin can withstand (Note: The indicated gauge pressure of a liquid chromatography apparatus may be measuring the total system pressure rather than the pressure drop across the column.)

More Product Data
Using antibody-binding proteins for antibody purification

Related Products
Pierce™ Protein A Plus Agarose
NAb™ Protein A Plus Spin Columns, 1 mL

NAb™ Protein A Plus Spin Column, 5 mL Thermo Scientific™

The Thermo Scientific Protein A Plus Agarose NAb Spin Columns are convenient for rapid, small-scale affinity purification of antibodies from a variety of sample types. Each column containing 5 mL of the immobilized protein resin enables quick purification of 5-65 mg of IgG from 2-10 mL of serum or other sample. The actual amount of IgG purified varies depending upon the sample type and the specific spin column used.

Pierce Protein A Plus Agarose is a versatile, high-performance affinity resin for antibody purification. It consists of purified native Protein A that has been covalently immobilized at high density onto high-quality crosslinked 6% beaded agarose (CL-6B). Among the many available varieties of immobilized Protein A affinity resins, this one provides the most versatile combination of chromatographic features for high yield and high purity purification of whole IgG from mammalian serum samples. The agarose beads have physical and chemical properties suitable for many affinity purification systems.

Features of Pierce Protein A Plus Agarose:

Native Protein A – immobilized Protein A is ideal for polyclonal IgG purification from human, rabbit, pig, dog or cat serum
Agarose resin – support is crosslinked 6% beaded agarose (CL-6B), the most popular resin for protein affinity purification methods
Inert and stable – superior manufacturing method immobilizes Protein A by charge-free, leach-resistant covalent bonds, resulting in low nonspecific binding and enabling multiple uses without decline in yield
High capacity – this “Plus” variety of Pierce Protein A Agarose has a dense load of immobilized Protein A, providing a binding capacity greater than 34 mg human IgG/mL resin (approx. 16 to 17 mg mouse IgG/mL resin)

Protein A is a cell wall component produced by several strains of Staphylococcus aureus. The 46.7kDa-protein consists of a single polypeptide chain that is essentially devoid of carbohydrate. Native Protein A has contains four high-affinity (Ka = 10^8/mol) binding sites that are capable of interacting with the Fc region of IgG-class antibodies from selected mammalian species. IgG-binding function is optimal at pH 8.2, but efficient binding also occurs in neutral and physiological buffers (pH 7.0 to 7.6).

Protein A is a bacteria-derived protein that binds with high affinity and specificity to the Fc portion of antibodies, especially those of the IgG class. Compared to its alternative (Protein G), Protein A provides the highest binding capacity for subclasses of IgG from rabbits, pigs, dogs and cats. Protein A also has higher binding capacity for human IgG, except for IgG3, which it binds weakly. Protein A binds weakly to mouse IgG1 and is not recommended for most applications with that antibody isotype.

Pierce Protein A Plus Agarose is prepared using Thermo Scientific AminoLink Coupling Chemistry, which provides several advantages compared to traditional methods of ligand immobilization. AminoLink Immobilization results in conjugation between sugar monomers of the agarose beads and native lysine residues on the Protein A via simple amide bonds. Unlike typical cyanogen bromide (CNBr) immobilization, the AminoLink Method does not introduce any novel chemical groups that could cause undesired nonspecific binding and produces a stable, essentially irreversible bond. The result is a high-binding-capacity resin that retains functional immobilized Protein A through multiple rounds of antibody purification.

Properties of crosslinked 6% beaded agarose (CL-6B):
• Support pH Stability: 2 to 14 (short term); 3 to 13 (long term)
• Average Particle Size: 45 to 165 microns
• Exclusion Limit: 10,000 to 4,000,000 daltons
• Maximum Volumetric Flow Rate: approx. 1 mL/minute (for 1 cm diameter column)
• Maximum Linear Velocity: 30 cm per hour
• Maximum Pressure: less than 25psi (1.5 bar), defined as the maximum pressure drop across a column that the resin can withstand (Note: The indicated gauge pressure of a liquid chromatography apparatus may be measuring the total system pressure rather than the pressure drop across the column.)

More Product Data
Using antibody-binding proteins for antibody purification

Related Products
Pierce™ Protein A Plus Agarose

POROS™ MabCapture™ A RoboColumn Thermo Scientific™

RoboColumn™ chromatography products are small columns provided in 8-column strips. They are useful for fully automated and parallel chromatographic separations using robotic liquid handling platforms. These particular RoboColumns contain POROS™ MabCapture™ A affinity resin, which is suitable for monoclonal antibody large-scale downstream bioprocessing.

POROS MabCapture A is a 50-micron affinity resin that combines improvements made in bead and chemistry technology. These advances provide a monoclonal antibody purification resin that not only demonstrates the highest dynamic binding performance at low flow rates, but also one that can maintain superior binding capacity at high linear velocities where other chromatography resins products cannot operate.

Features of the resin:
• Obtain the highest dynamic binding capacity for monoclonal antibodies vs. conventional agarose or silica based Protein A medias
• Maintain high dynamic binding capacity at flow rates over 700 cm/hr
• Design processes with greater flexibility (shorter bed heights, faster flow rates or both) due to the improvements made to the antibody binding efficiency
• Clean and sanitize with standard sodium hydroxide agents utilized for other chromatography products

RoboColumn is a registered trademark of ATOLL GmbH.

NAb™ Protein G Spin Columns, 5 mL Thermo Scientific™

The Thermo Scientific NAb Protein G Spin Columns are convenient for rapid, small-scale affinity purification of antibodies from a variety of sample types. Each column containing 5 mL of the immobilized protein resin enables quick purification of 5-65 mg of IgG from 2-10 mL of serum or other sample. The actual amount of IgG purified varies depending upon the sample type and the specific spin column used.

Pierce Protein G Agarose is a premium-quality affinity resin for antibody purification. It consists of recombinant Protein G that has been covalently immobilized onto high-quality crosslinked 6% beaded agarose (CL-6B). This particular variety of the affinity resin provides the most versatile combination of chromatographic features for high yield and high-purity purification of whole IgG from mammalian serum samples. The agarose beads have physical and chemical properties suitable for many affinity purification systems.

Features of Pierce Protein G Agarose:

Protein G – immobilized Protein G is ideal for polyclonal IgG purification from mouse, human, cow, goat and sheep serum, including human IgG3 and mouse IgG1 isotypes
Agarose resin – support is crosslinked 6% beaded agarose (CL-6B), the most popular resin for protein affinity purification methods
Inert and stable – superior manufacturing method immobilizes Protein G by charge-free, leach-resistant covalent bonds, resulting in low nonspecific binding and enabling multiple uses without decline in yield
Standard capacity – Pierce Protein G Agarose has a normal load of immobilized Protein G, providing a binding capacity of 11 to 15 mg human IgG/mL resin

Protein G is a bacterial cell wall protein original from group G Streptococcus and now produced as a recombinant in E. coli. Like Protein A, Protein G binds to most mammalian immunoglobulins primarily through their Fc regions. Native Protein G contains two immunoglobulin binding sites, as well as albumin and cell surface binding sites. In the recombinant form of Protein G, these albumin and cell surface binding sites have been eliminated to reduce nonspecific binding when purifying immunoglobulins. Recombinant Protein G has a mass of approximately 21.6kDa, but its apparent size by SDS-PAGE is 31 to 34kDa. IgG-binding function is optimal at pH 5 but also occurs efficiently in near-neutral conditions (pH 7.0 to 7.2).

Compared to Protein A, Protein G binds a broader spectrum of IgG subclasses from human, mouse and rat serum. In particular, besides binding other isotypes just as well as Protein A, Protein G exhibits stronger binding to human IgG3, mouse IgG1 and all three isotypes of Rat IgG. Thus, Protein G is generally recommended for applications involving these species and isotypes of antibody. With regard to other species, Protein G provides higher-capacity binding for IgG from cow, goat and sheep, but it binds more weakly than Protein A to pig, guinea pig, dog and cat IgG.

Pierce Protein G Agarose is prepared using Thermo Scientific AminoLink Coupling Chemistry, which provides several advantages compared to traditional methods of ligand immobilization. AminoLink Immobilization results in conjugation between sugar monomers of the agarose beads and native lysine residues on the Protein A via simple amide bonds. Unlike typical cyanogen bromide (CNBr) immobilization, the AminoLink Method does not introduce any novel chemical groups that could cause undesired nonspecific binding and produces a stable, essentially irreversible bond. The result is a high-binding-capacity resin that retains functional immobilized Protein G through multiple rounds of antibody purification.

Pierce Protein G Agarose is effective for affinity purification of IgG from serum and other fluids of many mammalian species. Protein G is especially well suited for use with mouse antibodies (including IgG1) in addition to most IgG isotypes from human, goat and sheep samples.

Properties of crosslinked 6% beaded agarose (CL-6B):
• Support pH Stability: 2 to 14 (short term); 3 to 13 (long term)
• Average Particle Size: 45 to 165 microns
• Exclusion Limit: 10,000 to 4,000,000 daltons
• Maximum Volumetric Flow Rate: approx. 1 mL/minute (for 1 cm diameter column)
• Maximum Linear Velocity: 30 cm per hour
• Maximum Pressure: less than 25psi (1.5 bar), defined as the maximum pressure drop across a column that the resin can withstand (Note: The indicated gauge pressure of a liquid chromatography apparatus may be measuring the total system pressure rather than the pressure drop across the column.)

More Product Data
Using antibody-binding proteins for antibody purification

Related Products
Pierce™ Protein G Agarose
Pierce™ Protein G Chromatography Cartridges, 5 mL

Pierce™ Protein G Chromatography Cartridges, 5 mL Thermo Scientific™

The Thermo Scientific™ Pierce™ Chromatography Cartridges Protein G are convenient, ready-to-use prepacked devices for isolation and purification of mammalian polyclonal and monoclonal antibodies from serum, ascites and cell culture supernatants.

Pierce Protein G Agarose consists of recombinant Protein G that has been covalently immobilized onto high-quality crosslinked 6% beaded agarose (CL-6B). This particular variety of the affinity resin provides the most versatile combination of chromatographic features for high yield and high purity purification of whole IgG from mammalian serum samples. The agarose beads have physical and chemical properties suitable for many affinity purification systems.

Features of Pierce Protein G Agarose:

Protein G – immobilized Protein G is ideal for polyclonal IgG purification from mouse, human, cow, goat and sheep serum, including human IgG3 and mouse IgG1 isotypes
Agarose resin – support is crosslinked 6% beaded agarose (CL-6B), the most popular resin for protein affinity purification methods
Inert and stable – superior manufacturing method immobilizes Protein G by charge-free, leach-resistant covalent bonds, resulting in low nonspecific binding and enabling multiple uses without decline in yield
Standard capacity – Pierce Protein G Agarose has a normal load of immobilized Protein G, providing a binding capacity of 11 to 15 mg human IgG/mL resin

Pierce Chromatography Cartridges are compatible with the major automated liquid-chromatography systems or for manual syringe processing, and attach directly to ÄKTA™ or FPLC Systems without additional connectors. An accessory pack, included with each product, readily adapts columns for use with Luer-Lok Syringe Fittings or 1/16” tubing. The Protein G cartridges provide fast, easy and reproducible chromatographic separations and can be regenerated for multiple uses.

Protein G is a bacterial cell wall protein original from group G Streptococcus and now produced as a recombinant in E. coli. Like Protein A, Protein G binds to most mammalian immunoglobulins primarily through their Fc regions. Native Protein G contains two immunoglobulin binding sites, as well as albumin and cell surface binding sites. In the recombinant form of Protein G, these albumin and cell surface binding sites have been eliminated to reduce nonspecific binding when purifying immunoglobulins. Recombinant Protein G has a mass of approximately 21.6kDa, but its apparent size by SDS-PAGE is 31 to 34kDa. IgG-binding function is optimal at pH 5 but also occurs efficiently in near-neutral conditions (pH 7.0 to 7.2).

Compared to Protein A, Protein G binds a broader spectrum of IgG subclasses from human, mouse and rat serum. In particular, besides binding other isotypes just as well as Protein A, Protein G exhibits stronger binding to human IgG3, mouse IgG1 and all three isotypes of Rat IgG. Thus, Protein G is generally recommended for applications involving these species and isotypes of antibody. With regard to other species, Protein G provides higher-capacity binding for IgG from cow, goat and sheep, but it binds more weakly than Protein A to pig, guinea pig, dog and cat IgG.

Pierce Protein G Agarose is prepared using Thermo Scientific AminoLink Coupling Chemistry, which provides several advantages compared to traditional methods of ligand immobilization. AminoLink Immobilization results in conjugation between sugar monomers of the agarose beads and native lysine residues on the Protein A via simple amide bonds. Unlike typical cyanogen bromide (CNBr) immobilization, the AminoLink Method does not introduce any novel chemical groups that could cause undesired nonspecific binding and produces a stable, essentially irreversible bond. The result is a high-binding-capacity resin that retains functional immobilized Protein

Pierce™ Protein A IgG Purification Kit, 1 mL Thermo Scientific™

Thermo Scientific Pierce Protein A Agarose is a standard-capacity Protein A beaded agarose resin for use in a variety of antibody affinity purification methods. This Protein A IgG Purification Kit contains 5 Protein A Columns (1 mL each), Protein A IgG Binding Buffer, IgG Elution Buffer and desalting columns.

Features of Protein A Agarose:

Native Protein A – immobilized Protein A is ideal for polyclonal IgG purification from human, rabbit, pig, dog or cat serum
Agarose resin – support is crosslinked 6% beaded agarose (CL-6B), the most popular resin for protein affinity purification methods
Inert and stable – superior manufacturing method immobilizes Protein A by charge-free, leach-resistant covalent bonds, resulting in low nonspecific binding and enabling multiple uses without decline in yield
Standard capacity – Pierce Protein A Agarose has a normal load of immobilized Protein A, providing a binding capacity of 12 to 19 mg human IgG/mL resin (approx. 6 to 8 mg mouse IgG/mL resin)

Pierce Protein A Agarose consists of purified native Protein A that has been covalently immobilized onto high-quality crosslinked 6% beaded agarose. The agarose beads have physical and chemical properties that enable them to be used in a variety of batch- or column-type affinity purification procedure.

Protein A is a cell wall component produced by several strains of Staphylococcus aureus. The 46.7kDa-protein consists of a single polypeptide chain that is essentially devoid of carbohydrate. Native Protein A has contains four high-affinity (Ka = 10^8/mol) binding sites that are capable of interacting with the Fc region of IgG-class antibodies from selected mammalian species. IgG-binding function is optimal at pH 8.2, but efficient binding also occurs in neutral and physiological buffers (pH 7.0 to 7.6).

Protein A is a bacteria-derived protein that binds with high affinity and specificity to the Fc portion of antibodies, especially those of the IgG class. Compared to its alternative (Protein G), Protein A provides the highest binding capacity for subclasses of IgG from rabbits, pigs, dogs and cats. Protein A also has higher binding capacity for human IgG, except for IgG3, which it binds weakly. Protein A also binds weakly to mouse IgG1 and is not recommended for most applications with that antibody isotype.

Pierce Protein A Agarose is prepared using Thermo Scientific AminoLink Coupling Chemistry, which provides several advantages compared to traditional methods of ligand immobilization. AminoLink Immobilization results in conjugation between sugar monomers of the agarose beads and native lysine residues on the Protein A via simple amide bonds. Unlike typical cyanogen bromide (CNBr) immobilization, the AminoLink Method does not introduce any novel chemical groups that could cause undesired nonspecific binding and produces a stable, essentially irreversible bond. The result is a high-binding-capacity resin that retains functional immobilized Protein A through multiple rounds of antibody purification.

Properties of crosslinked 6% beaded agarose (CL-6B):
• Support pH Stability: 2 to 14 (short term); 3 to 13 (long term)
• Average Particle Size: 45 to 165 microns
• Exclusion Limit: 10,000 to 4,000,000 daltons
• Maximum Volumetric Flow Rate: approx. 1 mL/minute (for 1 cm diameter column)
• Maximum Linear Velocity: 30 cm per hour
• Maximum Pressure: less than 25psi (1.5 bar), defined as the maximum pressure drop across a column that the resin can withstand (Note: The indicated gauge pressure of a liquid chromatography apparatus may be measuring the total system pressure rather than the pressure drop across the column.)

Related Products
Pierce™ Protein A Agarose
Pierce™ Protein A Columns, 1 mL

NAb™ Protein A/G Spin Kit, 1 mL Thermo Scientific™

This Thermo Scientific NAb Protein A/G Spin Kit is convenient for rapid, small-scale affinity purification of antibodies from a variety of sample types. Included in each kit are sufficient NAb Protein A/G Spin Columns, collection tubes, buffers and a streamlined protocol for purifying at least 2 antibody samples.

Features of Pierce Protein A/G Agarose:

Protein A/G – immobilized recombinant fusion protein of the antibody-binding domains of Protein A and Protein G enables polyclonal IgG purification from nearly any mammalian species
Agarose resin – support is crosslinked 6% beaded agarose (CL-6B), the most popular resin for protein affinity purification methods
Inert and stable – superior manufacturing method immobilizes Protein A/G by charge-free, leach-resistant covalent bonds, resulting in low nonspecific binding and enabling multiple uses without decline in yield
Standard capacity – Pierce Protein A/G Agarose has a normal load of immobilized Protein A/G, providing a binding capacity greater than 7 mg human IgG/mL resin

Pierce Protein A/G Agarose consists of purified Protein A/G recombinant fusion protein that has been covalently immobilized onto high-quality crosslinked 6% beaded agarose (CL-6B). This particular variety of the resin provides the most versatile combination of chromatographic features for high yield and high purity purification of whole IgG from mammalian serum samples. The agarose beads have physical and chemical properties suitable for many affinity purification systems.

Protein A/G is a genetically-engineered protein that combines the IgG binding domains of both Protein A and Protein G. The fusion protein is expressed in E. coli. Protein A/G contains four Fc binding domains from Protein A and two from Protein G, resulting in a final mass of 50,460 daltons (40 to 45kDa by SDS-PAGE). Protein A/G is not as pH-dependent as Protein A alone, but otherwise has the additive properties of Protein A and G.

Protein A/G binds to all human IgG subclasses, making it the ideal choice for purification of polyclonal or monoclonal IgG antibodies whose subclass identities have not been determined. In addition, it binds to IgA, IgE, IgM and (to a lesser extent) IgD. Protein A/G also binds well to all mouse IgG subclasses but does not bind mouse IgA, IgM or serum albumin. This makes Protein A/G an excellent tool for purification and detection of mouse monoclonal antibodies from IgG subclasses, without interference from IgA, IgM and murine serum albumin. Individual subclasses of mouse monoclonals are likely to have a stronger affinity to the chimeric Protein A/G than to either Protein A or Protein G.

Pierce Protein A/G Agarose is prepared using Thermo Scientific AminoLink Coupling Chemistry, which provides several advantages compared to traditional methods of ligand immobilization. AminoLink Immobilization results in conjugation between sugar monomers of the agarose beads and native lysine residues on the Protein A via simple amide bonds. Unlike typical cyanogen bromide (CNBr) immobilization, the AminoLink Method does not introduce any novel chemical groups that could cause undesired nonspecific binding and produces a stable, essentially irreversible bond. The result is a high-binding-capacity resin that retains functional immobilized Protein A/G through multiple rounds of antibody purification.

Pierce Protein A/G Agarose binds nearly all isotypes and mammalian species of IgG from serum, ascites fluid, cell culture supernantant and other antibody samples, thereby enabling effective antibody purification. Because the immobilized Protein A/G combines the immunoglobulin-binding domains of both Protein A and Protein G, the resin is effective for affinity purification of IgG antibodies from a broad range mammalian host species.

Properties of crosslinked 6% beaded agarose (CL-6B):
• Support pH Stability: 2 to 14 (short term); 3 to 13 (long term)
• Average Particle Size: 45 to 165 microns
• Exclusion Limit: 10,000 to 4,000,000 daltons
• Maximum Volumetric Flow Rate: approx. 1 mL/minute (for 1 cm diameter column)
• Maximum Linear Velocity: 30 cm per hour
• Maximum Pressure: less than 25psi (1.5 bar), defined as the maximum pressure drop across a column that the resin can withstand (Note: The indicated gauge pressure of a liquid chromatography apparatus may be measuring the total system pressure rather than the pressure drop across the column.)

More Product Data
Using antibody-binding proteins for antibody purification

Related Products
Pierce™ Protein A/G Agarose
NAb™ Protein A/G Spin Columns, 1 mL

POROS™ A 20 µm Column, 2.1 x 30 mm, 0.1 mL Thermo Scientific™

Applied Biosystems™ POROS™ Prepacked Protein A, Affinity Column. POROS™ Protein A is based on an immobilized recombinant Protein A functional group designed for high throughput purification of antibodies.
20 micron particle size is used for high resolution and small scale preparative to semi-preparative separation of biomolecules.

POROS™ High Performance Chromatography™ Media and Pre-Packed Columns

A high performance chromatography resins for analytical to process scale separations.• Higher productivity: High throughput and high dynamic capacity associated with High Performance Chromatography™
• Chemical stability: Allows aggressive cleaning and sanitization
• Enhanced biomolecule access: Provided via large pores, ranging between 500-10000 Å
• Polystyrenedivinylbenzene particles: Yield a robust, easily packable matrix
• Develop better separations methods in a shorter time frame: The speed of High Performance Chromatography technology reduces weeks of experimentation to only a few hours of work, so you have plenty of time to explore all the variables of your separation

Reduce time-consuming sample prep

You can replace many sample preparation steps with high speed High Performance Chromatography technology. For instance, dialysis of large volumes of material can be replaced by high flow rate processing of the dilute sample. Elution in a small volume of new buffer accomplishes both buffer exchange and concentration.

Create novel assays for more efficient analysis

High Performance Chromatography technology is not limited to standard modes of separation. Both enzymes and affinity ligands can be immobilized on POROS media. By combining rapid on-column protein digestions with rapid on-column immunoassays and chromatographic separations, you can create entirely new assays with unlimited potential.

High Capacity, High Resolution, High Speed

In contrast to conventional chromatography media, POROS™ High Performance Chromatography™ resins particles, are engineered to have two discreet classes of pores. Large "throughpores" allow convection flow to occur through the particles themselves, quickly carrying sample molecules to short "diffusive" pores inside. By reducing the distance over which diffusion needs to occur, the time required for sample molecules to interact with interior binding sites is also reduced. Diffusion is no longer limiting and flow rates can be dramatically increased - without compromising resolution or capacity. Separations can be achieved at 1,000 to 5,000 cm⁄hr compared to 50 to 360 cm⁄hr for conventional media.

NAb™ Protein A/G Spin Column, 5 mL Thermo Scientific™

The Thermo Scientific NAb Protein A/G Spin Columns are convenient for rapid, small-scale affinity purification of antibodies from a variety of sample types.

Features of Pierce Protein A/G Agarose:

Protein A/G – immobilized recombinant fusion protein of the antibody-binding domains of Protein A and Protein G enables polyclonal IgG purification from nearly any mammalian species
Agarose resin – support is crosslinked 6% beaded agarose (CL-6B), the most popular resin for protein affinity purification methods
Inert and stable – superior manufacturing method immobilizes Protein A/G by charge-free, leach-resistant covalent bonds, resulting in low nonspecific binding and enabling multiple uses without decline in yield
Standard capacity – Pierce Protein A/G Agarose has a normal load of immobilized Protein A/G, providing a binding capacity greater than 7 mg human IgG/mL resin

Pierce Protein A/G Agarose consists of purified Protein A/G recombinant fusion protein that has been covalently immobilized onto high-quality crosslinked 6% beaded agarose (CL-6B). This particular variety of the resin provides the most versatile combination of chromatographic features for high yield and high purity purification of whole IgG from mammalian serum samples. The agarose beads have physical and chemical properties suitable for many affinity purification systems.

Protein A/G is a genetically-engineered protein that combines the IgG binding domains of both Protein A and Protein G. The fusion protein is expressed in E. coli. Protein A/G contains four Fc binding domains from Protein A and two from Protein G, resulting in a final mass of 50,460 daltons (40 to 45kDa by SDS-PAGE). Protein A/G is not as pH-dependent as Protein A alone, but otherwise has the additive properties of Protein A and G.

Protein A/G binds to all human IgG subclasses, making it the ideal choice for purification of polyclonal or monoclonal IgG antibodies whose subclass identities have not been determined. In addition, it binds to IgA, IgE, IgM and (to a lesser extent) IgD. Protein A/G also binds well to all mouse IgG subclasses but does not bind mouse IgA, IgM or serum albumin. This makes Protein A/G an excellent tool for purification and detection of mouse monoclonal antibodies from IgG subclasses, without interference from IgA, IgM and murine serum albumin. Individual subclasses of mouse monoclonals are likely to have a stronger affinity to the chimeric Protein A/G than to either Protein A or Protein G.

Pierce Protein A/G Agarose is prepared using Thermo Scientific AminoLink Coupling Chemistry, which provides several advantages compared to traditional methods of ligand immobilization. AminoLink Immobilization results in conjugation between sugar monomers of the agarose beads and native lysine residues on the Protein A via simple amide bonds. Unlike typical cyanogen bromide (CNBr) immobilization, the AminoLink Method does not introduce any novel chemical groups that could cause undesired nonspecific binding and produces a stable, essentially irreversible bond. The result is a high-binding-capacity resin that retains functional immobilized Protein A/G through multiple rounds of antibody purification.

Pierce Protein A/G Agarose binds nearly all isotypes and mammalian species of IgG from serum, ascites fluid, cell culture supernantant and other antibody samples, thereby enabling effective antibody purification. Because the immobilized Protein A/G combines the immunoglobulin-binding domains of both Protein A and Protein G, the resin is effective for affinity purification of IgG antibodies from a broad range mammalian host species.

Properties of crosslinked 6% beaded agarose (CL-6B):
• Support pH Stability: 2 to 14 (short term); 3 to 13 (long term)
• Average Particle Size: 45 to 165 microns
• Exclusion Limit: 10,000 to 4,000,000 daltons
• Maximum Volumetric Flow Rate: approx. 1 mL/minute (for 1 cm diameter column)
• Maximum Linear Velocity: 30 cm per hour
• Maximum Pressure: less than 25psi (1.5 bar), defined as the maximum pressure drop across a column that the resin can withstand (Note: The indicated gauge pressure of a liquid chromatography apparatus may be measuring the total system pressure rather than the pressure drop across the column.)

More Product Data
Using antibody-binding proteins for antibody purification

Related Products
Pierce™ Protein A/G Agarose
Pierce™ Protein A/G Chromatography Cartridges, 5 mL

Pierce™ Protein A Spin Plate for IgG Screening Thermo Scientific™

The Thermo Scientific Pierce Protein A Plus Agarose Spin Plate for IgG Screening offers a high-throughput format for quick purification and screening of antibodies and for immunoprecipitation (IP). Each well of the 96-well spin plate can process 10-100 µl of serum, cell culture supernatant or ascites fluid samples.

Pierce Protein A Plus Agarose is a versatile, high-performance affinity resin for antibody purification. It consists of purified native Protein A that has been covalently immobilized at high density onto high-quality crosslinked 6% beaded agarose (CL-6B). Among the many available varieties of immobilized Protein A affinity resins, this one provides the most versatile combination of chromatographic features for high yield and high purity purification of whole IgG from mammalian serum samples. The agarose beads have physical and chemical properties suitable for many affinity purification systems.

Features of Pierce Protein A Plus Agarose:

Native Protein A – immobilized Protein A is ideal for polyclonal IgG purification from human, rabbit, pig, dog or cat serum
Agarose resin – support is crosslinked 6% beaded agarose (CL-6B), the most popular resin for protein affinity purification methods
Inert and stable – superior manufacturing method immobilizes Protein A by charge-free, leach-resistant covalent bonds, resulting in low nonspecific binding and enabling multiple uses without decline in yield
High capacity – this “Plus” variety of Pierce Protein A Agarose has a dense load of immobilized Protein A, providing a binding capacity greater than 34 mg human IgG/mL resin (approx. 16 to 17 mg mouse IgG/mL resin)

Protein A is a cell wall component produced by several strains of Staphylococcus aureus. The 46.7kDa-protein consists of a single polypeptide chain that is essentially devoid of carbohydrate. Native Protein A has contains four high-affinity (Ka = 10^8/mol) binding sites that are capable of interacting with the Fc region of IgG-class antibodies from selected mammalian species. IgG-binding function is optimal at pH 8.2, but efficient binding also occurs in neutral and physiological buffers (pH 7.0 to 7.6).

Protein A is a bacteria-derived protein that binds with high affinity and specificity to the Fc portion of antibodies, especially those of the IgG class. Compared to its alternative (Protein G), Protein A provides the highest binding capacity for subclasses of IgG from rabbits, pigs, dogs and cats. Protein A also has higher binding capacity for human IgG, except for IgG3, which it binds weakly. Protein A binds weakly to mouse IgG1 and is not recommended for most applications with that antibody isotype.

Pierce Protein A Plus Agarose is prepared using Thermo Scientific AminoLink Coupling Chemistry, which provides several advantages compared to traditional methods of ligand immobilization. AminoLink Immobilization results in conjugation between sugar monomers of the agarose beads and native lysine residues on the Protein A via simple amide bonds. Unlike typical cyanogen bromide (CNBr) immobilization, the AminoLink Method does not introduce any novel chemical groups that could cause undesired nonspecific binding and produces a stable, essentially irreversible bond. The result is a high-binding-capacity resin that retains functional immobilized Protein A through multiple rounds of antibody purification.

Properties of crosslinked 6% beaded agarose (CL-6B):
• Support pH Stability: 2 to 14 (short term); 3 to 13 (long term)
• Average Particle Size: 45 to 165 microns
• Exclusion Limit: 10,000 to 4,000,000 daltons
• Maximum Volumetric Flow Rate: approx. 1 mL/minute (for 1 cm diameter column)
• Maximum Linear Velocity: 30 cm per hour
• Maximum Pressure: less than 25psi (1.5 bar), defined as the maximum pressure drop across a column that the resin can withstand (Note: The indicated gauge pressure of a liquid chromatography apparatus may be measuring the total system pressure rather than the pressure drop across the column.)

More Product Data
Using antibody-binding proteins for antibody purification

Related Products
Pierce™ Protein A Plus Agarose

Pierce™ Protein L Chromatography Cartridges, 1 mL Thermo Scientific™

The Thermo Scientific™ Pierce™ Chromatography Cartridges Protein L are convenient, ready-to-use prepacked devices for isolation and purification of immunoglobulin classes IgG, IgM, IgA, IgE and IgD via their kappa light chains.

Pierce Protein L Agarose consists of recombinant Protein L that has been covalently immobilized onto high-quality crosslinked 6% beaded agarose (CL-6B). This particular variety of the resin provides the most versatile combination of chromatographic features for high yield and high purity purification of target immunoglobulins. The agarose beads have physical and chemical properties suitable for many affinity purification systems.

Features of Pierce Protein L Agarose:

Protein L – immobilized Protein L is ideal for selective purification of human and mouse antibodies that have kappa light chains
Agarose resin – support is crosslinked 6% beaded agarose (CL-6B), the most popular resin for protein affinity purification methods
Inert and stable – superior manufacturing method immobilizes Protein L by charge-free, leach-resistant covalent bonds, resulting in low nonspecific binding and enabling multiple uses without decline in yield
Standard capacity – Pierce Protein L Agarose has a normal load of immobilized Protein L, providing a binding capacity of 5 to 10 mg human IgG/mL resin

Pierce Chromatography Cartridges are compatible with the major automated liquid-chromatography systems or for manual syringe processing, and attach directly to ÄKTA™ or FPLC Systems without additional connectors. An accessory pack, included with each product, readily adapts columns for use with Luer-Lok Syringe Fittings or 1/16” tubing. Protein L cartridges provide fast, easy and reproducible chromatographic separations and can be regenerated for multiple uses.

Applications:
• Purify monoclonal and polyclonal antibodies that bind poorly to Protein A or Protein G agarose
• Purify monoclonal antibodies from culture supernatants supplemented with bovine serum
• Protein L immunoprecipitation and co-immunoprecipitation assays
• Purify ScFv and Fab fragments containing kappa light chains

Protein L is an immunoglobulin-binding protein (36kDa) that originates from the bacteria Peptostreptococcus magnus, but is now produced recombinantly in E. coli. Unlike Protein A and Protein G, which bind primarily through Fc regions (i.e., heavy chain) of immunoglobilins, Protein L binds Igs through interactions with the light chains. Because no part of the heavy chain is involved in the binding interaction, Protein L binds a wider range of Ig classes than Protein A or G. Protein L binds to representatives of all classes of Ig, including IgG, IgM, IgA, IgE and IgD. Single chain variable fragments (ScFv) and Fab fragments also bind to Protein L.

Despite this wide-ranging binding capability with respect to Ig classes, Protein L is not a universal immunoglobilin-binding protein. Binding of Protein L to immunoglobulins is restricted to those containing kappa light chains (i.e., kappa chain of the VL domain). In humans and mice, kappa light chains predominate. The remaining immunoglobulins have lambda light chains. Furthermore, Protein L is effective in binding only certain subtypes of kappa light chains. For example, it binds human VkI, VkIII and VkIV subtypes but does not bind the VkII subtype. Binding of mouse immunoglobulins is restricted to those having VkI light chains.

Given these specific requirements for effective binding, the main application for immobilized Protein L is purification of monoclonal antibodies from ascites or culture supernatant that are known to have the kappa light chain. Protein L is extremely useful for purification of VLk-containing monoclonal antibodies from culture supernatant because it does not bind bovine immunoglobi

Pierce™ Protein L Agarose Thermo Scientific™

The Thermo Scientific NAb Protein L Spin Columns are convenient for rapid, small-scale affinity purification of antibodies from a variety of sample types. Each column containing 5 mL of the immobilized protein resin enables quick purification of 5-65 mg of IgG from 2-10 mL of serum or other sample. The actual amount of IgG purified varies depending upon the sample type and the specific spin column used.

Pierce Protein L Agarose consists of recombinant Protein L that has been covalently immobilized onto high-quality crosslinked 6% beaded agarose (CL-6B). This particular variety of the resin provides the most versatile combination of chromatographic features for high yield and high purity purification of target immunoglobulins. The agarose beads have physical and chemical properties suitable for many affinity purification systems.

Features of Pierce Protein L Agarose:

Protein L – immobilized Protein L is ideal for selective purification of human and mouse antibodies that have kappa light chains
Agarose resin – support is crosslinked 6% beaded agarose (CL-6B), the most popular resin for protein affinity purification methods
Inert and stable – superior manufacturing method immobilizes Protein L by charge-free, leach-resistant covalent bonds, resulting in low nonspecific binding and enabling multiple uses without decline in yield
Standard capacity – Pierce Protein L Agarose has a normal load of immobilized Protein L, providing a binding capacity of 5 to 10 mg human IgG/mL resin

Applications:
• Purify monoclonal and polyclonal antibodies that bind poorly to Protein A or Protein G agarose
• Purify monoclonal antibodies from culture supernatants supplemented with bovine serum
• Protein L immunoprecipitation and co-immunoprecipitation assays
• Purify ScFv and Fab fragments containing kappa light chains

Protein L is an immunoglobulin-binding protein (36kDa) that originates from the bacteria Peptostreptococcus magnus, but is now produced recombinantly in E. coli. Unlike Protein A and Protein G, which bind primarily through Fc regions (i.e., heavy chain) of immunoglobilins, Protein L binds Igs through interactions with the light chains. Because no part of the heavy chain is involved in the binding interaction, Protein L binds a wider range of Ig classes than Protein A or G. Protein L binds to representatives of all classes of Ig, including IgG, IgM, IgA, IgE and IgD. Single chain variable fragments (ScFv) and Fab fragments also bind to Protein L.

Despite this wide-ranging binding capability with respect to Ig classes, Protein L is not a universal immunoglobilin-binding protein. Binding of Protein L to immunoglobulins is restricted to those containing kappa light chains (i.e., kappa chain of the VL domain). In humans and mice, kappa light chains predominate. The remaining immunoglobulins have lambda light chains. Furthermore, Protein L is effective in binding only certain subtypes of kappa light chains. For example, it binds human VkI, VkIII and VkIV subtypes but does not bind the VkII subtype. Binding of mouse immunoglobulins is restricted to those having VkI light chains.

Given these specific requirements for effective binding, the main application for immobilized Protein L is purification of monoclonal antibodies from ascites or culture supernatant that are known to have the kappa light chain. Protein L is extremely useful for purification of VLk-containing monoclonal antibodies from culture supernatant because it does not bind bovine immunoglobilins, which are present in the media serum supplement. Also, Protein L does not interfere with the antigen-binding site of the antibody, making it useful for immunoprecipitation assays, even using IgM.

Pierce Protein L Agarose is prepared using Thermo Scientific AminoLink Coupling Chemistry, which provides several advantages compared to traditional methods of ligand immobilization. AminoLink Immobilization results in conjugation between sugar monomers of the agarose beads and native lysine residues on the Protein A via simple amide bonds. Unlike typical cyanogen bromide (CNBr) immobilization, the AminoLink Method does not introduce any novel chemical groups that could cause undesired nonspecific binding and produces a stable, essentially irreversible bond. The result is a high-binding-capacity resin that retains functional immobilized Protein L through multiple rounds of antibody purification.

Pierce Protein L Agarose is a versatile affinity resin for purification of mammalian IgG isotypes that contain specific kappa light chains. Protein L is especially suited for isolating mouse IgG VkI and human IgG VkI, VkIII and VkIV monoclonal antibodies.

Properties of crosslinked 6% beaded agarose (CL-6B):
• Support pH Stability: 2 to 14 (short term); 3 to 13 (long term)
• Average Particle Size: 45 to 165 microns
• Exclusion Limit: 10,000 to 4,000,000 daltons
• Maximum Volumetric Flow Rate: approx. 1 mL/minute (for 1 cm diameter column)
• Maximum Linear Velocity: 30 cm per hour
• Maximum Pressure: less than 25psi (1.5 bar), defined as the maximum pressure drop across a column that the resin can withstand (Note: The indicated gauge pressure of a liquid chromatography apparatus may be measuring the total system pressure rather than the pressure drop across the column.)

More Product Data
Using antibody-binding proteins for antibody purification

Related Products
Pierce™ Protein L Agarose
Pierce™ Protein L Chromatography Cartridges, 5 mL

Pierce™ Protein A/G Chromatography Cartridges, 5 mL Thermo Scientific™

The Thermo Scientific™ Pierce™ Chromatography Cartridges Protein A/G are convenient, ready-to-use prepacked devices for isolation and purification of most immunoglobins.

Pierce Protein A/G Agarose consists of purified Protein A/G recombinant fusion protein that has been covalently immobilized onto high-quality crosslinked 6% beaded agarose (CL-6B). This particular variety of the resin provides the most versatile combination of chromatographic features for high yield and high purity purification of whole IgG from mammalian serum samples. The agarose beads have physical and chemical properties suitable for many affinity purification systems.

Features of Protein A/G Agarose:

Protein A/G – immobilized recombinant fusion protein of the antibody-binding domains of Protein A and Protein G enables polyclonal IgG purification from nearly any mammalian species
Agarose resin – support is crosslinked 6% beaded agarose (CL-6B), the most popular resin for protein affinity purification methods
Inert and stable – superior manufacturing method immobilizes Protein A/G by charge-free, leach-resistant covalent bonds, resulting in low nonspecific binding and enabling multiple uses without decline in yield
Standard capacity – Pierce Protein A/G Agarose has a normal load of immobilized Protein A/G, providing a binding capacity greater than 7 mg human IgG/mL resin

Pierce Chromatography Cartridges are compatible with the major automated liquid-chromatography systems or for manual syringe processing, and attach directly to ÄKTA™ or FPLC Systems without additional connectors. An accessory pack, included with each product, readily adapts cartridges for use with Luer-Lok Syringe Fittings or 1/16” tubing. The Protein A/G cartridges provide fast, easy and reproducible chromatographic separations and can be regenerated for multiple uses.

Protein A/G is a genetically-engineered protein that combines the IgG binding domains of both Protein A and Protein G. The fusion protein is expressed in E. coli. Protein A/G contains four Fc binding domains from Protein A and two from Protein G, resulting in a final mass of 50,460 daltons (40 to 45kDa by SDS-PAGE). Protein A/G is not as pH-dependent as Protein A alone, but otherwise has the additive properties of Protein A and G.

Protein A/G binds to all human IgG subclasses, making it the ideal choice for purification of polyclonal or monoclonal IgG antibodies whose subclass identities have not been determined. In addition, it binds to IgA, IgE, IgM and (to a lesser extent) IgD. Protein A/G also binds well to all mouse IgG subclasses but does not bind mouse IgA, IgM or serum albumin. This makes Protein A/G an excellent tool for purification and detection of mouse monoclonal antibodies from IgG subclasses, without interference from IgA, IgM and murine serum albumin. Individual subclasses of mouse monoclonals are likely to have a stronger affinity to the chimeric Protein A/G than to either Protein A or Protein G.

Pierce Protein A/G Agarose is prepared using Thermo Scientific AminoLink Coupling Chemistry, which provides several advantages compared to traditional methods of ligand immobilization. AminoLink Immobilization results in conjugation between sugar monomers of the agarose beads and native lysine residues on the Protein A via simple amide bonds. Unlike typical cyanogen bromide (CNBr) immobilization, the AminoLink Method does not introduce any novel chemical groups that could cause undesired nonspecific binding and produces a stable, essentially irreversible bond. The result is a high-binding-capacity resin that retains functional immobilized Protein A/G through multiple rounds of antibody purification.

Pierce Protein A/G Agarose binds nearly all isotypes and mammalian species of IgG from serum, ascites fluid, cell culture supernantant and other ant

POROS™ MabCapture™ A Select RoboColumn Thermo Scientific™

RoboColumn™ chromatography products are small columns provided in 8-column strips. They are useful for fully automated and parallel chromatographic separations using robotic liquid handling platforms. These particular RoboColumns contain POROS™ MabCapture™ A Select affinity resin, which is suitable for monoclonal antibody large scale downstream bioprocessing.

POROS MabCapture A Select affinity resin is a the latest high-throughput, lower-priced Protein A chromatography resin, combining past improvements to MabCapture A with our own Protein A ligand, enabling the same high-throughput purification performance at a significant reduction in price over most Protein A chromatography resins . These advances provide a monoclonal antibody purification resin that demonstrates high dynamic binding performance at low flow rates and maintains superior binding capacity at high linear velocities where other chromatography resins products cannot operate.

Features of the resin:
• Obtain the highest dynamic binding capacity for monoclonal antibodies vs. conventional agarose or silica based Protein A medias
• Maintain high dynamic binding capacity at flow rates over 700 cm/hr
• Design processes with greater flexibility (shorter bed heights, faster flow rates or both) due to the improvements made to the antibody binding efficiency
• Clean and sanitize with standard sodium hydroxide agents utilized for other chromatography products
• Recognize major increases to process productivity and reduce the downstream bottleneck

RoboColumn is a registered trademark of ATOLL GmbH.

NAb™ Protein L Spin Kit, 1 mL Thermo Scientific™

The Thermo Scientific™ NAb™ Protein L Spin Kits are convenient for rapid, small-scale affinity purification of antibodies from a variety of sample types. Each pre-filled microcentrifuge spin column of the immobilized protein resin enables quick purification of 5-10 mg of human IgG from 0.5-2 mL of serum or other sample. Included in each kit are sufficient collection tubes, buffers and a streamlined protocol for purifying at least two antibody samples.

Pierce Protein L Agarose consists of recombinant Protein L that has been covalently immobilized onto high-quality crosslinked 6% beaded agarose (CL-6B). This particular variety of the resin provides the most versatile combination of chromatographic features for high yield and high purity purification of target immunoglobulins. The agarose beads have physical and chemical properties suitable for many affinity purification systems.

Features of Pierce Protein L Agarose:

Protein L – immobilized Protein L is ideal for selective purification of human and mouse antibodies that have kappa light chains
Agarose resin – support is crosslinked 6% beaded agarose (CL-6B), the most popular resin for protein affinity purification methods
Inert and stable – superior manufacturing method immobilizes Protein L by charge-free, leach-resistant covalent bonds, resulting in low nonspecific binding and enabling multiple uses without decline in yield
Standard capacity – Pierce Protein L Agarose has a normal load of immobilized Protein L, providing a binding capacity of 5 to 10 mg human IgG/mL resin

Applications:
• Purify monoclonal and polyclonal antibodies that bind poorly to Protein A or Protein G agarose
• Purify monoclonal antibodies from culture supernatants supplemented with bovine serum
• Protein L immunoprecipitation and co-immunoprecipitation assays
• Purify ScFv and Fab fragments containing kappa light chains

Protein L is an immunoglobulin-binding protein (36kDa) that originates from the bacteria Peptostreptococcus magnus, but is now produced recombinantly in E. coli. Unlike Protein A and Protein G, which bind primarily through Fc regions (i.e., heavy chain) of immunoglobilins, Protein L binds Igs through interactions with the light chains. Because no part of the heavy chain is involved in the binding interaction, Protein L binds a wider range of Ig classes than Protein A or G. Protein L binds to representatives of all classes of Ig, including IgG, IgM, IgA, IgE and IgD. Single chain variable fragments (ScFv) and Fab fragments also bind to Protein L.

Despite this wide-ranging binding capability with respect to Ig classes, Protein L is not a universal immunoglobilin-binding protein. Binding of Protein L to immunoglobulins is restricted to those containing kappa light chains (i.e., kappa chain of the VL domain). In humans and mice, kappa light chains predominate. The remaining immunoglobulins have lambda light chains. Furthermore, Protein L is effective in binding only certain subtypes of kappa light chains. For example, it binds human VkI, VkIII and VkIV subtypes but does not bind the VkII subtype. Binding of mouse immunoglobulins is restricted to those having VkI light chains.

Given these specific requirements for effective binding, the main application for immobilized Protein L is purification of monoclonal antibodies from ascites or culture supernatant that are known to have the kappa light chain. Protein L is extremely useful for purification of VLk-containing monoclonal antibodies from culture supernatant because it does not bind bovine immunoglobilins, which are present in the media serum supplement. Also, Protein L does not interfere with the antigen-binding site of the antibody, making it useful for immunoprecipitation assays, even using IgM.

Pierce Protein L Agarose is prepared using Thermo Scientific AminoLink Coupling Chemistry, which provides several advantages compared to traditional methods of ligand immobilization. AminoLink Immobilization results in conjugation between sugar monomers of the agarose beads and native lysine residues on the Protein A via simple amide bonds. Unlike typical cyanogen bromide (CNBr) immobilization, the AminoLink Method does not introduce any novel chemical groups that could cause undesired nonspecific binding and produces a stable, essentially irreversible bond. The result is a high-binding-capacity resin that retains functional immobilized Protein L through multiple rounds of antibody purification.

Pierce Protein L Agarose is a versatile affinity resin for purification of mammalian IgG isotypes that contain specific kappa light chains. Protein L is especially suited for isolating mouse IgG VkI and human IgG VkI, VkIII and VkIV monoclonal antibodies.

Properties of crosslinked 6% beaded agarose (CL-6B):
• Support pH Stability: 2 to 14 (short term); 3 to 13 (long term)
• Average Particle Size: 45 to 165 microns
• Exclusion Limit: 10,000 to 4,000,000 daltons
• Maximum Volumetric Flow Rate: approx. 1 mL/minute (for 1 cm diameter column)
• Maximum Linear Velocity: 30 cm per hour
• Maximum Pressure: less than 25psi (1.5 bar), defined as the maximum pressure drop across a column that the resin can withstand (Note: The indicated gauge pressure of a liquid chromatography apparatus may be measuring the total system pressure rather than the pressure drop across the column.)

More Product Data
Using antibody-binding proteins for antibody purification

Related Products
Pierce™ Protein L Agarose
NAb™ Protein L Spin Columns, 1 mL
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