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Genomics and Proteomics


Immunoassay Kit Format


Target Organism Class


Concanavalin A, Tetramethylrhodamine Conjugate Invitrogen™

Concanavalin A (Con A) is one of the most widely used lectins in cell biology. Our tetramethylrhodamine conjugate of Con A exhibits the bright, orange-red fluorescence of tetramethylrhodamine (absorption/emission maxima ~555/580 nm). Tetramethylrhodamine Con A selectively binds to α-mannopyranosyl and α-glucopyranosyl residues.

View complete list of fluorescent dye-conjugated lectins ›

Control BACULOSOMES™ Plus Reagent

Control BACULOSOMES® Plus Reagent is a microsomal preparation from insect cells infected with wild-type baculovirus. This product is suitable as a negative control for assays carried out using our CYP450 BACULOSOMES® Plus Reagents.

For Research Use Only. Not for any animal or human therapeutic or diagnostic use.

CellVue™ Burgundy Cell Labeling Kit Invitrogen™

CellVue™ dyes are lipophilic dyes that can be used to label the cell membrane for the purpose of identifying and tracking labeled cells. Cell labeling is rapid and stable and can be combined with fluorescently labeled antibodies and other markers of cellular function for flow cytometric analysis and fluorescent microscopy. The Mini CellVue™ Kits are supplied with one vial of dye stock (1 mM in ethanol) and one vial of labeling vehicle (Diluent C).

CellVue™ Burgundy is a far-red/near-infrared fluorescent cell labeling reagent. It is optimally excited at 683 nm and has a peak emission of 707 nm. CellVue™ Burgundy can be excited by the red laser line (633 nm), however, it can be detected equally in filter sets designed for Alexa Fluor™ 700 and APC-eFluor™ 780 or similar fluorochromes. Therefore, it is not recommended to use antibodies conjugated to these fluorochromes when using CellVue™ Burgundy. CellVue™ Burgundy can be used in combination with APC.

Reported Application
Flow Cytometric Analysis, Microscopy, Immunocytochemistry, Cell Labeling

Active Arf1 Pull-Down and Detection Kit Thermo Scientific™

The Thermo Scientific Pierce Active Arf1 Pull-Down and Detection Kit is a complete kit for selective enrichment and detection of GTP-bound Arf1 GTPase through specific protein interaction with the GGA3 protein-binding domain.

The Active Arf1 Pull-Down and Detection Kit includes purified GST-GGA3 protein-binding domain (PBD), glutathione agarose resin, positive and negative controls (GTPγS and GDP, respectively), lysis/binding/wash buffer, anti-Arf1 primary antibody, sample buffer, spin columns and collection tubes. The kit was validated using lysates from MDCK cells, a cell line that is known to have robust Arf1 activity.

Features of the Active Arf1 Pull-Down and Detection Kit:

Highly sensitive and accurate—optimized reagents, specific anti-Arf1 antibody and Western blot procedure ensure accurate controls and semi-quantitative results
Validated—functionally tested for Arf1 detection to ensure quality and performance
Compatible—effective with a variety of cell types from mouse, rat and human sources

Applications:
• Follow activation of Arf1 GTPase during cell differentiation, migration, division and cytoskeletal rearrangement
• Study the activation of Arf1 during the assembly of coat proteins onto budding vesicles or trans-golgi network and endosomes
• Monitor Arf1 activity after stimulation with growth factors
• Monitor Arf1 activity after small molecule inhibitor treatment

The Active Arf1 Pull-Down and Detection Kit was validated for function and specificity of the active Arf1 enrichment method using cell lysates treated with GTPγS to activate endogenous Arf1 and compared to lysates treated with GDP to inactivate the small GTPase. GTPγS treatment traps Arf1 in the GTP-bound form (active), resulting in a strong signal when endogenous Arf1 is present. GDP treatment pushes Arf1 into the GDP-bound state (inactive), resulting in minimal or no signal, regardless of Arf1 protein levels. The kit is optimized for Western blot detection using an HRP-conjugated secondary antibody (Goat Anti-rabbit IgG, Part No. 31460) and Thermo Scientific SuperSignal West Pico Chemiluminescent Substrate (Part No. 34080). The kit contains sufficient components for 30 pull-down assays.

Arf1 Background:
ADP ribosylation factor proteins 1-6 (Arfs) are members of the Ras family of small GTPases. Although structurally similar, the cellular roles of Arf1-6 are different from the other Arf family members; their endogenous roles are not ADP ribosylation, but rather regulation of heterotrimeric G proteins. The Arf proteins can be divided into three classes: Class I—Arf 1-3; Class II—Arf 4,5; Class III—Arf6. Class I and II Arfs are associated with trans-Golgi network (TGN) and are involved in recruiting effector proteins to the Golgi membrane and forming vesicles. Unlike other GTPases, Arf GTPases are modified by myristoylation at the amino-terminus to allow insertion into the membrane. The Class III protein Arf6 is associated with the plasma membrane and is involved in vesicle formation at the plasma membrane, vesicle recycling and remodeling of the actin cytoskeleton.

Although the Arf GTPases are expressed in all cells, Arf1 is the most abundant, active and best-characterized. Arf1 recruits its effectors, including coatomer and clathrin adaptor complex (AP), to the Golgi for vesicle formation and budding. After recruitment, Arf1 maintains the coat proteins on the membrane until the vesicle is formed. Arf1 then interacts with its effector resulting in the release of Arf1 from the membrane and vesicle budding. The golgi-localized γ-ear-containing Arf (GGA) effector binding proteins contain an amino-terminal VHS domain, a GGA homology domain (GGAH), a proline-rich linker region and a carboxy-terminal γ-ear adaptin homology domain (AGEH). The VHS domain interacts with cytoplasmic domains for receptor sorting, the GGAH domain binds activated Arf, the proline-rich region interacts with clathrin and the AGEH domain interacts with cytosolic proteins. Through mutational studies, the GGAH domain has been shown to be sufficient to bind GTP-bound Arf, and Arf1 binds all three GGA effector proteins. The association of the GGA proteins with the TGN and with the plasma membrane is regulated by the Arf.

More Product Data
Measure activation of small GTPases via their specific downstream effectors

Pierce™ Peroxide Assay Reagent A Thermo Scientific™

Thermo Scientific Pierce Peroxide Assay Reagent A is a formulation consisting of 25mM ammonium ferrous sulfate. This formulation is one of the component of Quantitative Peroxide Assay Kit used for detecting and measuring hydrogen peroxide levels based on the oxidation of ferrous to ferric ion in the presence of xylenol orange, present in the biological and other liquid samples.

Applications of Pierce Quantitative Peroxide Assay Kits:
• General hydrogen peroxide (H2O2) measurement in protein samples
• Quantitation of peroxides in detergents
• Assessment of the effects of peroxides on cellular activity
• Determination of protein glycation; Measurement of peroxide accumulation in lipids

In these assay kits, hydroperoxides convert the Fe2+ to Fe3+ at acidic pH. With the aqueous-compatible formulation, peroxide first reacts with sorbitol, converting it to a peroxyl radical, which in turn initiates Fe2+ oxidation to Fe3+. In the lipid compatible formulation, the peroxide converts the Fe2+ to Fe3+ directly. In a sulfuric acid solution, the Fe3+ complexes with the xylenol orange (XO) dye to yield a purple product having an absorbance maximum at 560nm. Peroxide levels in test samples can be determined by calculation from the know extinction coefficient of the XO-Fe complex or by reference to a standard curve prepared with hydrogen peroxide solution.Most proteins do not interfere with these assays, although some metal chelators may require use of a blank (i.e., control) to mitigate these effects.


Related Products
Pierce™ Quantitative Peroxide Assay Kit (Aqueous)
Pierce™ Quantitative Peroxide Assay Kit (Lipid)
Pierce™ Peroxide Assay Reagent B
Pierce™ Peroxide Assay Reagent C

ViaGram™ Red+ Bacterial Gram Stain and Viability Kit Invitrogen™

The ViaGram™ Red+ Bacterial Gram Stain and Viability Kit provides a simple fluorescent staining protocol for fluorescence microscopy that differentially stains many gram-positive and gram-negative bacterial species and, at the same time, discriminates live from dead cells on the basis of plasma membrane integrity.

CellVue™ Lavender Cell Labeling Kit Invitrogen™

CellVue™ dyes are lipophilic dyes that can be used to label the cell membrane for the purpose of identifying and tracking labeled cells. Cell labeling is rapid and stable and can be combined with fluorescently labeled antibodies and other markers of cellular function for flow cytometric analysis and fluorescent microscopy. Mini CellVue™ Kits are supplied with one vial of dye stock (1 mM in ethanol) and one vial of labeling vehicle (Diluent C).

CellVue™ Lavender is a violet fluorescent cell labeling reagent. It is optimally excited at 420 nm and has a peak emission of 461 nm. CellVue™ Lavender is compatible with most multi-color flow cytometric applications; however, due to similar emission properties it cannot be used in combination with eFluor™ 450 or Pacific Blue™ reagents.

Reported Application
Flow Cytometric Analysis, Microscopy, Immunocytochemistry, Cell Labeling

Z'-LYTE™ Kinase Assay Kit - Ser/Thr 16 Peptide

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon CDC25C, containing Ser-198.

Pierce™ Peroxide Assay Reagent B Thermo Scientific™

Thermo Scientific Pierce Peroxide Assay Reagent B is a formulation consisting of 125µM xylenol orange in water with sorbitol. This formulation is one of the component of Quantitative Peroxide Assay Kit used for detecting and measuring hydrogen peroxide levels based on the oxidation of ferrous to ferric ion in the presence of xylenol orange, present in the biological and other liquid samples.

Features of Peroxide Assay Reagent B:

Convenient—no proprietary reagents involved and remove the variability, difficulty and added expense of formulating the reagents yourself
Aqueous-compatible formulations— the formulation includes sorbitol, which provides enhanced sensitivity with non-lipid samples

Applications of Pierce Quantitative Peroxide Assay Kits:
• General hydrogen peroxide (H2O2) measurement in protein samples
• Quantitation of peroxides in detergents
• Assessment of the effects of peroxides on cellular activity
• Determination of protein glycation; Measurement of peroxide accumulation in lipids

In these assay kits, hydroperoxides convert the Fe2+ to Fe3+ at acidic pH. With the aqueous-compatible formulation, peroxide first reacts with sorbitol, converting it to a peroxyl radical, which in turn initiates Fe2+ oxidation to Fe3+. In the lipid compatible formulation, the peroxide converts the Fe2+ to Fe3+ directly. In a sulfuric acid solution, the Fe3+ complexes with the xylenol orange (XO) dye to yield a purple product having an absorbance maximum at 560nm. Peroxide levels in test samples can be determined by calculation from the know extinction coefficient of the XO-Fe complex or by reference to a standard curve prepared with hydrogen peroxide solution.Most proteins do not interfere with these assays, although some metal chelators may require use of a blank (i.e., control) to mitigate these effects.

Related Products
Pierce™ Quantitative Peroxide Assay Kit (Aqueous)
Pierce™ Quantitative Peroxide Assay Kit (Lipid)
Pierce™ Peroxide Assay Reagent A
Pierce™ Peroxide Assay Reagent C

Pro-Detect™ Rapid V5 Competitive Assay Kit Thermo Scientific™

The Thermo Scientific Pro-Detect Rapid V5 Competitive Assay Kit is a single-use, dipstick lateral flow kit for quick, easy detection of V5-tagged proteins in protein expression and purification systems. This lateral flow assay uses membrane-based strips coated with dye-bound conjugated antibodies specific to V5 tag to provide a qualitative detection within ten minutes. This assay can be used to detect V5-tagged proteins directly from cell culture media (supernatant) or cell lysates without any special instrumentation or handling.

Features of the Pro-Detect Rapid competitive assay kits:
Easy to adopt—lateral flow strips can be used early in and throughout expression to monitor and confirm expression of desired protein-tagged target
Simple, fast method—simply dilute sample using provided diluent and dip in the lateral flow strip for qualitative results within 5-10 minutes
Sensitive—detect protein tags in supernatant or cell lysate samples down to low µg/mL concentrations
Specific—assay does not cross-react with common lysis and protein preparation reagents
Reliable—results are similar to other antibody immunoassays, including western blot and ELISA
Long shelf life—stable for at least one year at 4°C

Each Pro-Detect Rapid competitive assay is performed by simply adding a properly diluted tissue culture supernatant, lysate, or purified protein sample to a test tube or microplate well and dipping in a lateral flow strip. Gold-conjugated detection antibodies embedded in the strip form complexes with the tagged protein in the sample. In the presence of target protein tag, the dye-bound conjugated antibodies will bind to the tagged protein present in the sample instead of the protein tag bound at the test lines. Therefore, in a positive result, a competitive lateral flow assay will show decreasing intensity of the visible test lines until all test lines have disappeared. When there is no target protein tag present, the dye-bound conjugated antibodies specific to the target will bind at the test line(s), resulting in visual test lines in a negative result.

TurboFect™ Transfection Reagent Thermo Scientific™

Thermo Scientific TurboFect Transfection Reagent is a highly efficient, easy-to-use, non-immunogenic transfection reagent for delivery of DNA plasmids and expression vectors into eukaryotic cells.

Features of TurboFect Transfection Reagent:

• High transfection efficiency of a wide variety of cell types
• No optimization required for most cell lines
• Excellent transfection efficiency in the presence or absence of serum
• Minimal cytotoxicity
• Ready-to-use—no need to reconstitute, dilute or manipulate

Applications of TurboFect Transfection Reagent :
• Stable and transient transfection or co-transfection of DNA or RNA
• Experiments with adherent or suspension cells, differentiated or undifferentiated cells
• Gene expression studies and assays involving GFP or luciferase reporters

TurboFect Transfection Reagent is a sterile solution of a proprietary cationic polymer. TurboFect Reagent interacts with DNA to form small, stable, highly diffusible complexes which are readily endocytosed. The reagent creates a proton-sponge effect that buffers endosomal pH by provoking massive proton accumulation and passive chloride influx. As a result, rapid osmotic swelling of the endocytosed DNA-complex causes it to rupture in the cytosol, allowing translocation of DNA to the nucleus. TurboFect Reagent is ideal for transfection of a variety of cells, including primary and difficult-to-transfect cells.

pMCS-Gaussia Luc Vector for Luciferase Assays Thermo Scientific™

The Thermo Scientific pMCS-Gaussia Luc vector is a multiple cloning site plasmid designed to accept a promoter sequence for study of gene regulation using the naturally secreting Gaussia luciferase reporter.

Features of the pMCS-Gaussia Luc vector:

• Naturally-secreting Gaussia luciferase gene, optimized for high expression in mammalian systems
• Multiple cloning site (MCS) provides versatility for transfer of regulatory elements from one plasmid to another
• Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through
• Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively
• High-copy pUC bacterial DNA replication origin

The Gaussia Luc Vectors contain a gene cloned from the copepod, Gaussia princeps. The gene encodes a naturally-secreted bioluminescent Gaussia luciferase (approx. 20kDa), which enables measurement of the reporter activity in media (for real-time assays) and in cell lysates. The pMCS vector contains a multiple cloning site for cloning a promoter to study its regulatory potential.

These vectors are subject to a limited use label license.

More Product Data
Highly sensitive multiplex luciferase reporter assays
Luciferase assays in hard-to-transfect Jurkat cells
Monitoring neuronal differentiation using multiplexed luciferase reporters
Activation of the antioxidant response pathway by pesticide chemicals

Related Products
pCMV-Gaussia Luc Vector for Luciferase Assays
pTK-Gaussia Luc Vector for Luciferase Assays

CellVue™ NIR815 Cell Labeling Kit Invitrogen™

CellVue™ dyes are lipophilic dyes that can be used to label the cell membrane for the purpose of identifying and tracking labeled cells. Cell labeling is rapid and stable and can be combined with fluorescently labeled antibodies and other markers of cellular function for flow cytometric analysis and fluorescent microscopy. Mini CellVue™ Kits are supplied with one vial of dye stock (1 mM in ethanol) and one vial of labeling vehicle (Diluent C).

CellVue™ NIR815 is a near-infrared fluorescent cell labeling reagent. It is optimally excited at 786 nm and has a peak emission of 814 nm. CellVue™ NIR815 has been reported to be useful for short-term in vivo cell tracking studies using a Pearl™ Imager or Odyssey Infrared Imaging System (Li-COR Biosciences, Inc., Lincoln, NE).

Reported Application
Microscopy, Immunocytochemistry, Cell Labeling

FAST DiO™ Solid; DiOΔ9,12-C18(3), ClO4 (3,3'-Dilinoleyloxacarbocyanine Perchlorate) Invitrogen™

FAST DiO is an unsaturated analog of DiO (DiOC18(3)) that reportedly migrates ~50% faster. It is weakly fluorescent in water but highly fluorescent and quite photostable when incorporated into membranes. It has an extremely high extinction coefficient and short excited-state lifetimes (~1 nanosecond) in lipid environments. Once applied to cells, the dye diffuses laterally within the plasma membrane.

Alexa Fluor™ 532 Phalloidin Invitrogen™

Alexa Fluor® 532 phalloidin is a high-affinity F-actin probe conjugated to our bright, photostable, yellow fluorescent Alexa Fluor® 532 dye.

Selectively stains F-actin
Outstanding fluorescence performance
Excitation/Emission: 531/554 nm
Superior to antibody staining
Optimal for fixed and permeabilized samples

Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides 'death cap' mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while Alexa Fluor® 532 provides yellow fluorescence of unparalleled brightness and photostability. Demonstrating very little nonspecific staining, Alexa Fluor®532 phalloidin allows high-contrast discrimination of actin staining.

Use in Multiple Applications
Alexa Fluor® 532 phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Alexa Fluor® 532 phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot® nanocrystals, and other Alexa Fluor® conjugates including secondary antibodies.

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links
Read about labeling F-Actin with phallotoxins.
See the full line of Alexa Fluor® Dye products.
Create a Cellular Masterpiece with Alexa Fluor® Phalloidin using the Cell Paint Tool.
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