Shop All Antibody and Dye Based Assays

Concanavalin A, Tetramethylrhodamine Conjugate Invitrogen™

Concanavalin A (Con A) is one of the most widely used lectins in cell biology. Our tetramethylrhodamine conjugate of Con A exhibits the bright, orange-red fluorescence of tetramethylrhodamine (absorption/emission maxima ~555/580 nm). Tetramethylrhodamine Con A selectively binds to α-mannopyranosyl and α-glucopyranosyl residues.

View complete list of fluorescent dye-conjugated lectins ›

CellVue™ Burgundy Cell Labeling Kit Invitrogen™

CellVue™ dyes are lipophilic dyes that can be used to label the cell membrane for the purpose of identifying and tracking labeled cells. Cell labeling is rapid and stable and can be combined with fluorescently labeled antibodies and other markers of cellular function for flow cytometric analysis and fluorescent microscopy. The Mini CellVue™ Kits are supplied with one vial of dye stock (1 mM in ethanol) and one vial of labeling vehicle (Diluent C).

CellVue™ Burgundy is a far-red/near-infrared fluorescent cell labeling reagent. It is optimally excited at 683 nm and has a peak emission of 707 nm. CellVue™ Burgundy can be excited by the red laser line (633 nm), however, it can be detected equally in filter sets designed for Alexa Fluor™ 700 and APC-eFluor™ 780 or similar fluorochromes. Therefore, it is not recommended to use antibodies conjugated to these fluorochromes when using CellVue™ Burgundy. CellVue™ Burgundy can be used in combination with APC.

Reported Application
Flow Cytometric Analysis, Microscopy, Immunocytochemistry, Cell Labeling

ViaGram™ Red+ Bacterial Gram Stain and Viability Kit Invitrogen™

The ViaGram™ Red+ Bacterial Gram Stain and Viability Kit provides a simple fluorescent staining protocol for fluorescence microscopy that differentially stains many gram-positive and gram-negative bacterial species and, at the same time, discriminates live from dead cells on the basis of plasma membrane integrity.

CellVue™ Lavender Cell Labeling Kit Invitrogen™

CellVue™ dyes are lipophilic dyes that can be used to label the cell membrane for the purpose of identifying and tracking labeled cells. Cell labeling is rapid and stable and can be combined with fluorescently labeled antibodies and other markers of cellular function for flow cytometric analysis and fluorescent microscopy. Mini CellVue™ Kits are supplied with one vial of dye stock (1 mM in ethanol) and one vial of labeling vehicle (Diluent C).

CellVue™ Lavender is a violet fluorescent cell labeling reagent. It is optimally excited at 420 nm and has a peak emission of 461 nm. CellVue™ Lavender is compatible with most multi-color flow cytometric applications; however, due to similar emission properties it cannot be used in combination with eFluor™ 450 or Pacific Blue™ reagents.

Reported Application
Flow Cytometric Analysis, Microscopy, Immunocytochemistry, Cell Labeling

CellVue™ NIR815 Cell Labeling Kit Invitrogen™

CellVue™ dyes are lipophilic dyes that can be used to label the cell membrane for the purpose of identifying and tracking labeled cells. Cell labeling is rapid and stable and can be combined with fluorescently labeled antibodies and other markers of cellular function for flow cytometric analysis and fluorescent microscopy. Mini CellVue™ Kits are supplied with one vial of dye stock (1 mM in ethanol) and one vial of labeling vehicle (Diluent C).

CellVue™ NIR815 is a near-infrared fluorescent cell labeling reagent. It is optimally excited at 786 nm and has a peak emission of 814 nm. CellVue™ NIR815 has been reported to be useful for short-term in vivo cell tracking studies using a Pearl™ Imager or Odyssey Infrared Imaging System (Li-COR Biosciences, Inc., Lincoln, NE).

Reported Application
Microscopy, Immunocytochemistry, Cell Labeling

FAST DiO™ Solid; DiOΔ9,12-C18(3), ClO4 (3,3'-Dilinoleyloxacarbocyanine Perchlorate) Invitrogen™

FAST DiO is an unsaturated analog of DiO (DiOC18(3)) that reportedly migrates ~50% faster. It is weakly fluorescent in water but highly fluorescent and quite photostable when incorporated into membranes. It has an extremely high extinction coefficient and short excited-state lifetimes (~1 nanosecond) in lipid environments. Once applied to cells, the dye diffuses laterally within the plasma membrane.

Alexa Fluor™ 532 Phalloidin Invitrogen™

Alexa Fluor® 532 phalloidin is a high-affinity F-actin probe conjugated to our bright, photostable, yellow fluorescent Alexa Fluor® 532 dye.

Selectively stains F-actin
Outstanding fluorescence performance
Excitation/Emission: 531/554 nm
Superior to antibody staining
Optimal for fixed and permeabilized samples

Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides 'death cap' mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while Alexa Fluor® 532 provides yellow fluorescence of unparalleled brightness and photostability. Demonstrating very little nonspecific staining, Alexa Fluor®532 phalloidin allows high-contrast discrimination of actin staining.

Use in Multiple Applications
Alexa Fluor® 532 phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Alexa Fluor® 532 phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot® nanocrystals, and other Alexa Fluor® conjugates including secondary antibodies.

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links
Read about labeling F-Actin with phallotoxins.
See the full line of Alexa Fluor® Dye products.
Create a Cellular Masterpiece with Alexa Fluor® Phalloidin using the Cell Paint Tool.

LysoSensor™ Yellow/Blue DND-160 - Special Packaging Invitrogen™

LysoSensor™ Yellow/Blue DND-160 is a ratiometric probe that can be used to measure the pH of acidic organelles, such as lysosomes. The LysoSensor dye produces blue fluorescence in neutral environments, but changes to yellow fluorescence in more acidic environments. The pKa of LysoSensor Yellow/Blue DND-160 is ~4.2.

Wheat Germ Agglutinin, Oregon Green™ 488 Conjugate Invitrogen™

Wheat germ agglutinin (WGA) is one of the most widely used lectins in cell biology. Our Oregon Green® 488 conjugate of WGA exhibits the bright, green fluorescence of the Oregon Green® 488 dye (excitation/emission maxima ~496/524 nm). Oregon Green® 488 WGA binds to sialic acid and N-acetylglucosaminyl residues.

View complete list of fluorescent dye-conjugated lectins ›

SYTO™ 21 Green Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO Invitrogen™

The cell-permeant SYTO 21 green fluorescent nucleic acid stain exhibits bright, green fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Green Fluorescent Nucleic Acid Stain Sampler Kit #2 (S-7554) to enable researchers to find the most appropriate green-fluorescent SYTO stain for their system.

FM™ 1-43 Dye (N-(3-Triethylammoniumpropyl)-4-(4-(Dibutylamino) Styryl) Pyridinium Dibromide) Invitrogen™

FM 1-43 membrane probe is an excellent reagent both for identifying actively firing neurons and for investigating the mechanisms of activity-dependent vesicle cycling. This water-soluble dye, which is nontoxic to cells and virtually nonfluorescent in aqueous medium, is believed to insert into the outer leaflet of the cell membrane where it becomes intensely fluorescent. In a neuron that is actively releasing neurotransmitters, the dye becomes internalized within the recycled synaptic vesicles and the nerve terminals become brightly stained. The nonspecific staining of cell-surface membranes can simply be washed off prior to viewing. FM 1-43 membrane probe is also available as 1 mg in a single vial (T-3163). FM 1-43FX membrane probe (F-35355), an analog of FM 1-43 membrane probe that can be fixed in place using aldehyde-based fixatives, is also available.

LysoTracker™ Yellow HCK-123 - Special Packaging Invitrogen™

LysoTracker® Yellow HCK-123 is a yellow fluorescent dye that stains acidic compartments in live cells with excitation/emission maxima ~465/535 nm.

CellVue™ NIR780 Cell Labeling Kit Invitrogen™

CellVue™ dyes are lipophilic dyes that can be used to label the cell membrane for the purpose of identifying and tracking labeled cells. Cell labeling is rapid and stable and can be combined with fluorescently labeled antibodies and other markers of cellular function for flow cytometric analysis and fluorescent microscopy. Mini CellVue™ Kits are supplied with one vial of dye stock (1 mM in ethanol) and one vial of labeling vehicle (Diluent C); Midi CellVue™ Kits are supplied with two vials of dye stock (1 mM in ethanol) and six vials of Diluent C.

CellVue™ NIR780 is a near-infrared fluorescent cell labeling reagent. It can be excited off the red laser line (633 nm) and has a peak emission of 776 nm that can be detected in filter sets designed for APC-eFluor™ 780. CellVue™ NIR780 is compatible with most multi-color applications, however, due to similar emission properties it cannot be used in combination with APC-eFluor™ 780 or APC-Alexa Fluor™ 750 reagents.

Reported Application
Flow Cytometric Analysis, Microscopy, Immunocytochemistry, Cell Labeling

Oregon Green™ 488 1,2-Dihexadecanoyl-sn-Glycero-3-Phosphoethanolamine (Oregon Green™ 488 DHPE) Invitrogen™

The phospholipid, Oregon Green® 488 DHPE is labeled on the head group with the green-fluorescent Oregon Green® fluorophore with excitation/emission maxima ~501/526 nm. This fluorinated analog of fluorescein overcomes some of the key limitations of fluorescein, including greater photostability and a lower pKa (pKa ~4.7 versus 6.4 for fluorescein), making its fluorescence essentially pH insensitive in the physiological pH range.

Alexa Fluor™ Plus 555 Phalloidin Invitrogen™

Alexa Fluor Plus 555 Phalloidin is a high-affinity filamentous actin (F-actin) probe (phalloidin) conjugated to our bright, photostable, orange/TRITC fluorescent Alexa Fluor Plus 555 dye. For the best possible sensitivity and specificity, Alexa Fluor Plus 555 Phalloidin is synthesized to maximize the units of Alexa Fluor Plus 555 Phalloidin per length of F-actin.

View our complete selection of Alexa Fluor and Alexa Fluor Plus conjugated phalloidin for F-actin staining ›

• Selectively stains F-actin with 3–4 times the sensitivity of Alexa Fluor 555 Phalloidin
• Outstanding fluorescence performance for sharp actin cytoskeleton images
• Excitation/emission: 555/565 nm. Compatible with standard TRITC/RFP filter set or laser settings
• More sensitive and specific than antibody staining against actin
• Optimal for fixed and permeabilized samples

Enables superior results in your actin staining studies
Phalloidin is a bi-cyclic peptide belonging to a family of toxins originating from the deadly Amanita phalloides 'death cap' mushroom and commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has several advantages over antibodies for actin labeling, including virtually identical binding properties with actin from different species of plants and animals, and low non-specific binding. Phalloidin binds F-actin with high selectivity, while Alexa Fluor Plus 555 dye provides orange/TRITC fluorescence of unparalleled brightness and photostability.

Use in multiple applications
Alexa Fluor Plus 555 Phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Its staining is fully compatible with other fluorescent stains used in cellular analyses, including fluorescent proteins, Qdot nanocrystals, and other Alexa Fluor conjugates including secondary antibodies.

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