Reporter assays, reagents, and process components that leverage the light-emitting properties of luciferin substrates via the use of luciferase enzymes, a class of oxidative enzymes that produce bioluminescence. Products include cloning site vectors, flash assays, etc.
The Thermo Scientific Pierce Firefly Luciferase Glow Assay Kit provides a bright and stable bioluminescence signal (half-life greater than 2 hours) in the presence of firefly luciferase reporters for protein expression assays.
The Thermo Scientific Pierce Firefly One-Step Luc Glow Assay Kit measures firefly luciferase activity in mammalian cell culture using a single reagent-addition step, making it ideal for high-throughput screening (HTS) applications.
The Thermo Scientific Pierce Gaussia Luciferase Glow Assay Kit provides an extremely bright and stable bioluminescence signal (half-life greater than 1 hour), especially in the presence of Gaussia-Dura luciferase reporter.
The ATP-dependent oxidation of luciferin by luciferase produces light, and this system is employed as a reporter in plants, bacteria, and mammalian cells. Because chemiluminescent techniques are virtually background-free, this reporter gene system is ideal for detecting low-level gene expression.
The Thermo Scientific Pierce Luciferase Cell Lysis Buffer effectively lyses adherent cells or pelleted suspension cells for analysis of promoter expression in luciferase assays. Pierce Luciferase Cell Lysis Buffer is effective for preparing high-yield mammalian cell lysates from either adherent...
The Thermo Scientific pMCS-Renilla Luc vector is a multiple cloning site plasmid to designed to accept a promoter sequence for study of gene regulation using the intracellular Renilla luciferase reporter.
The Thermo Scientific pCMV-Gaussia-Dura Luc vector is a derivative of pMCS-Gaussia-Dura Luc. It contains a mutant form of the Gaussia luciferase gene (conferring better bioluminescent signal stability than the native luciferase) under the control of the strong Cytomegalovirus (CMV) promoter.
Thermo Scientific pTK-Red Firefly Luc is a constitutive expression vector having the luciferase gene under the Herpes Simplex Virus (HSV) thymidine kinase (TK) promoter for co-transfection and normalization control to account for experimental variation in combination with other reporters in a gene...
Thermo Scientific pMCS-Red Firefly Luc is a multiple cloning site plasmid designed to accept a promoter sequence for the study of gene regulation using the intracellular red firefly luciferase reporter with excellent light intensity..
Thermo Scientific pCMV-Red Firefly Luc is a constitutive expression vector having the luciferase gene under the CMV (Cytomegalovirus) promoter for co-transfection and normalization control to account for experimental variation in combination with other reporters in a gene regulation study using the...
The Thermo Scientific Pierce Renilla Luciferase Glow Assay Kit provides an extremely bright and stable bioluminescence signal (half-life approx. 3 hours), especially in the presence of Green Renilla luciferase reporter.
The Thermo Scientific pTK-Gaussia-Dura Luc vector is a derivative of pMCS-Gaussia-Dura Luc. It contains a mutant form of the Gaussia luciferase gene (conferring better bioluminescent signal stability than the native luciferase) under the control of the weak Herpes Simplex Virus (HSV) thymidine...
The ATP-dependent oxidation of luciferin by luciferase produces light, and this system is employed as a reporter in plants, bacteria, and mammalian cells. Because chemiluminescent techniques are virtually background-free, this reporter gene system is ideal for detecting low-level gene expression.
The Thermo Scientific Pierce Cypridina Luciferase Flash Assay Kit provides researchers with a highly sensitive assay for transcriptional activity of regulatory elements in mammalian cell culture media and whole cell lysate.
The Thermo Scientific Pierce Firefly Luciferase Flash Assay Kit provides reagents to measure Red firefly luciferase activity in whole cell producing a very strong flash signal which decays rapidly. Features of the Firefly Luciferase Flash Assay Kit: • Highly sensitive detection of firefly luciferase...