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General Cell Based Assays
General cell-based assays are used broadly to conduct live cell procedures and experiments in cell and molecular biology research settings.
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CellSensor™ NFκB-bla THP-1 Cell Line Invitrogen™
The CellSensor® NFκB-bla THP-1 cell line contains a beta-lactamase reporter gene under the control of the Nuclear Factor kappa B (NFκB) response element stably integrated into THP-1 cells. To obtain the cell line, the NFκB-bla construct was transduced into THP-1 cells by lentivirus.
CellSensor™ AP-1-bla ME-180 Cell Line Invitrogen™
The CellSensor® AP-1-bla ME-180 Cell Line contains a beta-lactamase reporter gene under control of the AP-1 response element stably integrated into ME-180 cells. The cell line was created through FACS sorting of cells responsive to stimulation of the AP-1 pathway with Epidermal Growth Factor (EGF).
CellSensor™ CRE-bla FreeStyle™ 293F Cell Line Thermo Scientific™
The CellSensor® CRE-bla FreeStyle™ 293F cell line contains a beta-lactamase reporter gene under control of the CRE response element stably integrated into FreeStyle™ 293F cells. To construct this cell line, the CRE -bla construct was transduced into FreeStyle™ 293F cells by lentivirus.
BacMam Histone H3K4me3 Cellular Assay Kit Invitrogen™
The BacMam Histone H3K4me3 Cellular Assay is a high-throughput screening (HTS) compatible TR-FRET assay for the interrogation of trimethylation at lysine 4 on histone H3 in a cellular format. Baculovirus-mediated gene delivery (BacMam) provides a convenient tool for the expression of GFP-Histone H3...
Tango™ EDG6-bla U2OS Cells Thermo Scientific™
The Tango™ EDG6-bla U2OS cells contain the human Endothelial Differentiation, Lysophosphatidic Acid G-protein-coupled Receptor, 6 (EDG6) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line.
T-REx™ Tango™ P2RY14-bla U2OS Cells Thermo Scientific™
T-REx™ Tango™ P2RY14-bla U2OS cells contain the human purinergic receptor P2Y14 (P2RY14) under doxycycline inducible control linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line.
GeneBLAzer™ PPAR gamma DA Assay Kit Thermo Scientific™
GeneBLAzer™PPAR gamma DA (Division Arrested) cells and PPAR gamma-UAS-bla HEK 293H cells contain the ligand-binding domain (LBD) of the human Peroxisome Proliferator-Activated Receptor-gamma (PPAR gamma) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293H...
CellSensor™ p53RE-bla HCT-116 Cell Line Thermo Scientific™
The CellSensor® p53RE-bla HCT-116 cell line contains a beta-lactamase reporter gene under control of the p53 response element stably integrated into HCT-116 cells. To construct this cell line, the p53RE-bla construct was transduced into HCT-116 cells by lentivirus.
GeneBLAzer™ ER Beta DA Assay Kit Thermo Scientific™
GeneBLAzer™ER beta DA(Division Arrested) cells and ER beta-UAS-bla GripTite™ 293 cells contain the ligand-binding domain (LBD) of the human Estrogen receptor beta (ER beta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla GripTite™ 293 cell line.
Kinase Tracer 1710, 25 µM
In the LanthaScreen™ Eu Kinase Binding Assay, binding of an Alexa Fluor™ conjugate or “tracer” such as Kinase Tracer 1710 to a kinase is detected by addition of a Eu-labeled anti-tag antibody. Binding of the tracer & antibody to a kinase results in a high degree of FRET, whereas displacement of the...
Tango™ GPR44 (CRTH2)-bla U2OS Cells Thermo Scientific™
Tango™ GPR44-bla U2OS cells contain the human G protein coupled receptor 44 (GPR44) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line.
Tango™ AGTR1-bla U2OS Cells Thermo Scientific™
Tango™ AGTR1-bla U2OS cells contain the human Angiotensin Receptor 1 (AGTR1) (AGTR1) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line.
Tango™ HTR2A-bla U2OS DA Assay Kit
The Tango™ HTR2A-bla U2OS DA cells contain the human Serotonin Type 2A receptor (HTR2A) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line.
GeneBLAzer™ ADORA3-Gα15-NFAT-bla CHO-K1 cells Thermo Scientific™
GeneBLAzer® ADORA3-Ga15-NFAT-bla CHO-K1 cells contain the human Adenosine A3 Receptor (ADORA3) stably integrated into the GeneBLAzer® Ga15-NFAT-bla CHO-K1 cell line. The GeneBLAzer® Ga15-NFAT-bla CHO-K1 cells (Cat. no.
Kinase Tracer 236, 50 µM Thermo Scientific™
In the LanthaScreen™ Eu Kinase Binding Assay, binding of an Alexa Fluor™ conjugate or “tracer" such as Kinase Tracer 236 to a kinase is detected by addition of a Eu-labeled anti-tag antibody. Binding of the tracer & antibody to a kinase results in a high degree of FRET, whereas displacement of the...
