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LyticBLAzer™-FRET B/G Homogeneous Kit Invitrogen™
LyticBLAzer™ -FRET B/G Substrate Kits provides a ratiometric, end-point method of analyzing beta-lactamase activity in cell lysates. The LyticBLAzer™ -FRET B/G Reagent combines the components for lysis and detection in a single reagent addition, resulting in a very simple assay. Use the LyticBLAzer™ on top-read or bottom-read instrumentation. LyticBLAzer™ Substrate Kits are available in homogeneous as well as non-homogeneous formats (Figure 1), each optimized for a different assay condition.
LyticBLAzer™ FRET B/G Substrate Kit Advantages:
• Ratiometric blue:green reporter readout reduces false hits by minimizing experimental noise
• Integrated lysis and detection components enable use with top-read or bottom read fluorescence instrumentation
• Endpoint analysis and stable fluorescence yield longer read times
LyticBLAzer™ -BODIPY™ FL Substrate Kit:
LyticBLAzer™ -BODIPY™ FL Substrate Kits provides an end-point, non-ratiometric method of analyzing beta-lactamase activity in cell lysates. The LyticBLAzer™ -BODIPY™ FL Reagent combines the components for lysis and detection in a single reagent addition, resulting in a very simple assay. Use the LyticBLAzer™ -BODIPY™ FL Assays on top-read or bottom-read instrumentation. LyticBLAzer™ -BODIPY™ FL Substrate Kits are available in homogeneous as well as non-homogeneous formats (Figure 1), each optimized for a different assay condition.
LyticBLAzer™ -BODIPY™ FL Substrate Kit Advantages:
• BODIPY™ fluorophore excitation avoids fluorescence interference caused by sample contaminants such as dust, lint, etc.
• Standard excitation and emission wavelengths are universally adaptable to all fluorescence instrumentation.
• Integrated lysis and detection components enable use with top-read or bottom read fluorescence instrumentation.
• Endpoint analysis and stable fluorescence yield longer read times

FlexMAP™ 3D™ Calibration Kit Invitrogen™

Assay Buffer (10X) Invitrogen™
For Research Use Only. Not for use in diagnostics procedures.

Z'-LYTE™ Kinase Assay Kit - Ser/Thr 9 Peptide

FlexMAP™ 3D™ Performance Verification Kit Invitrogen™

Vivid™ CYP3A4 Red Screening Kit
• Easy three-step procedure, 'mix and read' format, reactions at room temperature or 37°C
• Superior fluorescent properties and kinetics compared to conventional fluorogenic probes
• High signal-to-background ratio, broad dynamic range
• Compatible with multiple assay formats from 96-well to 1536-well
Simple Mix and Read Kit Format
Vivid® CYP450 Screening Kits include Vivid® Substrate, Vivid® Fluorescent Standard, reaction buffer, Vivid® Regeneration System, NADP+, and CYP450 BACULOSOMES® Plus Reagents. The CYP450 BACULOSOMES® Plus Reagents are microsomes prepared from insect cells expressing a human CYP450 isozyme (CYP3A4 in this case) and human cytochrome-P450 reductase. CYP450 BACULOSOMES® Plus Reagents offer a distinct advantage over human liver microsomes in that only one CYP450 isozyme is expressed, thereby preventing metabolism by other CYP450s.
Unique Vivid® Reagents for Bright Fluorescent Signals and Low Background
Vivid® Substrates are blocked dyes that yield minimal fluorescence signal until cleaved or hydroxylated. Oxidation at either of two potential sites releases the highly fluorescent product (Figure 1). They have superior fluorescence, solubility, and kinetic properties compared to conventional fluorogenic probes. This results in higher sensitivity, greater signal-to-noise ratio, and better assay reproducibility. Vivid® BOMR Substrate is the substrate included in this particular kit. This substrate yields a product that emits a red fluorescence.
Flexible Assay Formats for Optimized Results
The sensitivity of the Vivid® CYP450 assays allows detection of weak inhibitors and miniaturization to as little as 2 µl per reaction. Assays may be set up in kinetic mode or in end-point mode to facilitate multi-plate screening (Figure 2). Assays may be performed at room temperature or 37°C.
Applications: high-throughput screening of enzyme-drug interactions, compound profiling for drug inhibition of cytochrome P450 isozymes, generation of predictive SAR models to guide compound acquisition
For Research Use Only. Not for any animal or human therapeutic or diagnostic use.

Phosphate Sensor

Z'-LYTE™ Kinase Assay Kit - Ser/Thr 6 Peptide

Vivid™ CYP2E1 Blue Screening Kit
• Easy three-step procedure, 'mix and read' format, reactions at room temperature or 37°C
• Superior fluorescent properties and kinetics compared to conventional fluorogenic probes
• High signal-to-background ratio, broad dynamic range
• Compatible with multiple assay formats from 96-well to 1536-well
Simple Mix and Read Kit Format
Vivid® CYP450 Screening Kits include Vivid® Substrate, Vivid® Fluorescent Standard, reaction buffer, Vivid® Regeneration System, NADP+, and CYP450 BACULOSOMES® Plus Reagents. The CYP450 BACULOSOMES® Plus Reagents are microsomes prepared from insect cells expressing a human CYP450 isozyme (CYP2E1 in this case) and human cytochrome-P450 reductase. CYP450 BACULOSOMES® Plus Reagents offer a distinct advantage over human liver microsomes in that only one CYP450 isozyme is expressed, thereby preventing metabolism by other CYP450s.
Unique Vivid® Reagents for Bright Fluorescent Signals and Low Background
Vivid® Substrates are blocked dyes that yield minimal fluorescence signal until cleaved or hydroxylated. Oxidation at either of two potential sites releases the highly fluorescent product (Figure 1). They have superior fluorescence, solubility, and kinetic properties compared to conventional fluorogenic probes. This results in higher sensitivity, greater signal-to-noise ratio, and better assay reproducibility. Vivid® EOMCC Substrate is the substrate included in this particular kit. This substrate yields a product that emits a blue fluorescence.
Flexible Assay Formats for Optimized Results
The sensitivity of the Vivid® CYP450 assays allows detection of weak inhibitors and miniaturization to as little as 2 µl per reaction. Assays may be set up in kinetic mode or in end-point mode to facilitate multi-plate screening (Figure 2). Assays may be performed at room temperature or 37°C.
Applications: high-throughput screening of enzyme-drug interactions, compound profiling for drug inhibition of cytochrome P450 isozymes, generation of predictive SAR models to guide compound acquisition
For Research Use Only. Not for any animal or human therapeutic or diagnostic use.

cAMP-Screen Direct™ Cyclic AMP Immunoassay System Applied Biosystems™
• The highest sensitivity of any commercially available cAMP assay
• Hours of read time with little or no degradation of the signal
• A simple protocol with cells grown directly on the assay plate
• Useful for a wide range of receptor activation studies
High Sensitivity and Hours of Steady Glow Time
This chemiluminescent assay is designed to provide the highest sensitivity of any commercially available cAMP assay. As few as 60 femtomoles of cAMP can be detected. The assay has a wide dynamic range, detecting from 0.06 to 6,000 picomoles of cAMP without the need for sample dilution or manipulations such as acetylation. This is especially helpful in cell-based assays when measuring Gs- or Gi-coupled agonist or antagonist stimulation and/or inhibition. Intra-assay precision for duplicate samples is typically 5% or less. Once the substrate/enhancer formulation reaches glow signal, the plate can be read for hours with little or no degradation of the signal. This is useful in screens where several plates are compared with each other. In addition, the assay exhibits exceptionally low cross-reactivity with other adenosine-containing or cyclic nucleotides.
For Receptor Activation Studies
The cAMP-Screen Direct® system is designed for quantitation of cellular cAMP for functional assays of receptor activation. The assay has been used with established cell lines for functional measurements with endogenous receptors, cell lines with exogenously expressed ligand receptors on the cell surface, primary cell cultures, and tissues in response to treatment with the appropriate ligands. In addition it has been used for receptor characterization, orphan receptor ligand identification, and the characterization of novel chimeric receptors. The assay can be used for high-throughput screens for compounds that stimulate or interfere with these signal transduction pathways.
A Simple Protocol—No Lysate Transfer Required
The assay follows a simple protocol. Cells are seeded into plates, cultured, and treated with test compounds as desired. Cell lysates are prepared in either the presence or absence of culture media. Lysates are incubated with a cAMP-AP conjugate and an anti-cAMP antibody in a coated microplate; the resulting immune complexes are captured in the plate. In samples without cAMP, all of the cAMP-AP conjugate is captured on the coated surface, resulting in a high signal. In the presence of cAMP, the amount of cAMP-AP conjugate captured decreases as a result of competition for binding with unlabeled cAMP, causing a reduced signal; signal reduction is proportional to the amount of cAMP present in the cell lysate. After washing to remove unbound cAMP-AP, the chemiluminescent substrate is added, and the resulting glow signal is measured in a luminometer.
This cAMP-Screen Direct® system has all of the same benefits as the cAMP-Screen® system, but also eliminates the need to transfer cell lysates from a tissue culture plate to the precoated microplates because the cells can be grown directly in the microplates. This provides better assay precision with one less transfer step. Many different cell types have been grown successfully on the precoated microplates, which also have a clear bottom to allow monitoring of cells.
For Research Use Only. Not for human or animal therapeutic or diagnostic use.

CYP2J2 BACULOSOMES™ Plus Reagent, rHuman Thermo Scientific™
• Single human P450 isozyme for detailed drug metabolism studies
• High specific activity increases dynamic range and sensitivity
• No background metabolism for clear results
Single Overexpressed Human CYP450 Isozyme
CYP450 BACULOSOMES® Plus Reagents offer a distinct advantage over Human Liver Microsomes (HLMs) in that only one CYP450 isozyme is expressed, thereby preventing metabolism by other CYP450s or other classes of drug metabolizing enzymes.
High Activity Results in Increased Sensitivity
Compound rankings based on metabolism and inhibition profiles observed with CYP450 BACULOSOMES® Plus Reagents are very similar to those seen with HLMs for most compounds tested. However, CYP450 BACULOSOMES® Plus Reagents activity and metabolic rates with most substrates is significantly higher than those seen with HLMs. This results in a broad dynamic range and high sensitivity in assays utilizing CYP450 BACULOSOMES® Plus Reagents. The high level of activity and reproducibility of the enzyme component observed in the reaction with most probe substrates makes CYP450 BACULOSOMES® Plus Reagents well suited to high-throughput screening formats.
High Signal to Noise Ratio
No background metabolism by endogenous insect CYP450s has been detected with any of the Vivid® probe substrates tested so far.
Applications: isozyme identification, DMPK analysis, isozyme-specific metabolism, and inhibition screening
For Research Use Only. Not for any animal or human therapeutic or diagnostic use.

Vivid™ CYP3A5 Blue Screening Kit
• Easy three-step procedure, 'mix and read' format, reactions at room temperature or 37°C
• Superior fluorescent properties and kinetics compared to conventional fluorogenic probes
• High signal-to-background ratio, broad dynamic range
• Compatible with multiple assay formats from 96-well to 1536-well
Simple Mix and Read Kit Format
Vivid® CYP450 Screening Kits include Vivid® Substrate, Vivid® Fluorescent Standard, reaction buffer, Vivid® Regeneration System, NADP+, and CYP450 BACULOSOMES® Plus Reagents. The CYP450 BACULOSOMES® Plus Reagents are microsomes prepared from insect cells expressing a human CYP450 isozyme (CYP3A5 in this case) and human cytochrome-P450 reductase. CYP450 BACULOSOMES® Plus Reagents offer a distinct advantage over human liver microsomes in that only one CYP450 isozyme is expressed, thereby preventing metabolism by other CYP450s.
Unique Vivid® Reagents for Bright Fluorescent Signals and Low Background
Vivid® Substrates are blocked dyes that yield minimal fluorescence signal until cleaved or hydroxylated. Oxidation at either of two potential sites releases the highly fluorescent product (Figure 1). They have superior fluorescence, solubility, and kinetic properties compared to conventional fluorogenic probes. This results in higher sensitivity, greater signal-to-noise ratio, and better assay reproducibility. Vivid® BOMCC Substrate is the substrate included in this particular kit. This substrate yields a product that emits a blue fluorescence.
Flexible Assay Formats for Optimized Results
The sensitivity of the Vivid® CYP450 assays allows detection of weak inhibitors and miniaturization to as little as 2 µl per reaction. Assays may be set up in kinetic mode or in end-point mode to facilitate multi-plate screening (Figure 2). Assays may be performed at room temperature or 37°C.
Applications: high-throughput screening of enzyme-drug interactions, compound profiling for drug inhibition of cytochrome P450 isozymes, generation of predictive SAR models to guide compound acquisition
For Research Use Only. Not for any animal or human therapeutic or diagnostic use.

LyticBLAzer™-FRET B/G Kit, 10X Thermo Scientific™
LyticBLAzer™ -FRET B/G Substrate Kits provides a ratiometric, end-point method of analyzing beta-lactamase activity in cell lysates. The LyticBLAzer™ -FRET B/G Reagent combines the components for lysis and detection in a single reagent addition, resulting in a very simple assay. Use the LyticBLAzer™ on top-read or bottom-read instrumentation. LyticBLAzer™ Substrate Kits are available in homogeneous as well as non-homogeneous formats (Figure 1), each optimized for a different assay condition.
LyticBLAzer™ FRET B/G Substrate Kit Advantages:
• Ratiometric blue:green reporter readout reduces false hits by minimizing experimental noise
• Integrated lysis and detection components enable use with top-read or bottom read fluorescence instrumentation
• Endpoint analysis and stable fluorescence yield longer read times
LyticBLAzer™ -BODIPY™ FL Substrate Kit:
LyticBLAzer™ -BODIPY™ FL Substrate Kits provides an end-point, non-ratiometric method of analyzing beta-lactamase activity in cell lysates. The LyticBLAzer™ -BODIPY™ FL Reagent combines the components for lysis and detection in a single reagent addition, resulting in a very simple assay. Use the LyticBLAzer™ -BODIPY™ FL Assays on top-read or bottom-read instrumentation. LyticBLAzer™ -BODIPY™ FL Substrate Kits are available in homogeneous as well as non-homogeneous formats (Figure 1), each optimized for a different assay condition.
LyticBLAzer™ -BODIPY™ FL Substrate Kit Advantages:
• BODIPY™ fluorophore excitation avoids fluorescence interference caused by sample contaminants such as dust, lint, etc.
• Standard excitation and emission wavelengths are universally adaptable to all fluorescence instrumentation.
• Integrated lysis and detection components enable use with top-read or bottom read fluorescence instrumentation.
• Endpoint analysis and stable fluorescence yield longer read times

Kinase Tracer 178, 25 µM Thermo Scientific™

Vivid™ CYP3A4 Green Screening Kit
• Easy three-step procedure, 'mix and read' format, reactions at room temperature or 37°C
• Superior fluorescent properties and kinetics compared to conventional fluorogenic probes
• High signal-to-background ratio, broad dynamic range
• Compatible with multiple assay formats from 96-well to 1536-well
Simple Mix and Read Kit Format
Vivid® CYP450 Screening Kits include Vivid® Substrate, Vivid® Fluorescent Standard, reaction buffer, Vivid® Regeneration System, NADP+, and CYP450 BACULOSOMES® Plus Reagents. The CYP450 BACULOSOMES® Plus Reagents are microsomes prepared from insect cells expressing a human CYP450 isozyme (CYP3A4 in this case) and human cytochrome-P450 reductase. CYP450 BACULOSOMES® Plus Reagents offer a distinct advantage over human liver microsomes in that only one CYP450 isozyme is expressed, thereby preventing metabolism by other CYP450s.
Unique Vivid® Reagents for Bright Fluorescent Signals and Low Background
Vivid® Substrates are blocked dyes that yield minimal fluorescence signal until cleaved or hydroxylated. Oxidation at either of two potential sites releases the highly fluorescent product (Figure 1). They have superior fluorescence, solubility, and kinetic properties compared to conventional fluorogenic probes. This results in higher sensitivity, greater signal-to-noise ratio, and better assay reproducibility. Vivid® DBOMF Substrate is the substrate included in this particular kit. This substrate yields a product that emits a green fluorescence.
Flexible Assay Formats for Optimized Results
The sensitivity of the Vivid® CYP450 assays allows detection of weak inhibitors and miniaturization to as little as 2 µl per reaction. Assays may be set up in kinetic mode or in end-point mode to facilitate multi-plate screening (Figure 2). Assays may be performed at room temperature or 37°C.
Applications: high-throughput screening of enzyme-drug interactions, compound profiling for drug inhibition of cytochrome P450 isozymes, generation of predictive SAR models to guide compound acquisition
For Research Use Only. Not for any animal or human therapeutic or diagnostic use.