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Pierce™ 660nm Protein Assay Reagent Thermo Scientific™
The Pierce 660-nm Protein Assay Reagent is a ready-to-use, detergent- and reducing agent-compatible reagent to quickly measure total protein concentration. The assay is more linear than Coomassie-based Bradford assays and compatible with higher concentrations of most detergents, reducing agents, and other commonly used reagents.
See all available protein assays ›
Features of the 660-nm Protein Assay Kit include:
• Compatibility—works with a greater range of detergents and reducing agents than other dye-based assays
• Ready-to-use—single reagent with a simple mix-and-read protocol; no working reagent to prepare
• Linearity—produces standard curves that are more linear than with the Bradford assay method
• Assay format—assay may be performed in test tubes or microplates
• Sample volume—requires only 10 µL for microplate or 100 µL for the test tube procedures
• Storage—room temperature storage
The accessory Ionic Detergent Compatibility Reagent (IDCR) provides for even broader detergent compatibility, making this one of the only protein assays that is suitable for samples containing Laemmli SDS sample buffer with bromophenol blue. Although the Pierce 660-nm Protein Assay produces a higher level of protein-to-protein variation (37%) than other assays, such as the BCA Protein Assay, the simpler single-reagent format and broader substance compatibility make the Pierce 660-nm assay more convenient for many routine applications. The Pierce 660-nm Protein Assay can be performed in either a test tube or microplate format.
How the Pierce 660-nm Assay detects protein
The Pierce 660-nm Protein Assay is based on the binding of a proprietary dye-metal complex to protein in acidic conditions that causes a shift in the dye's absorption maximum, which is measured at 660 nm. The dye-metal complex is reddish-brown and changes to green upon protein binding. The color change is produced by deprotonation of the dye at low pH facilitated by interactions with positively charged amino acid groups in proteins. Therefore, the dye interacts mainly with basic residues in proteins, such as histidine, arginine, and lysine and to a lesser extent tyrosine, tryptophan, and phenylalanine.
The color produced in the assay is stable and increases in proportion to a broad range of increasing protein concentrations, even in the presence of detergents and reducing agents that would be incompatible with Bradford and BCA Protein Assays.
Related products
Pierce 660-nm Protein Assay Kit (includes protein standards)
Ionic Detergent Compatibility Reagent for Pierce 660-nm Protein Assay Reagent
See all available protein assays ›
Features of the 660-nm Protein Assay Kit include:
• Compatibility—works with a greater range of detergents and reducing agents than other dye-based assays
• Ready-to-use—single reagent with a simple mix-and-read protocol; no working reagent to prepare
• Linearity—produces standard curves that are more linear than with the Bradford assay method
• Assay format—assay may be performed in test tubes or microplates
• Sample volume—requires only 10 µL for microplate or 100 µL for the test tube procedures
• Storage—room temperature storage
The accessory Ionic Detergent Compatibility Reagent (IDCR) provides for even broader detergent compatibility, making this one of the only protein assays that is suitable for samples containing Laemmli SDS sample buffer with bromophenol blue. Although the Pierce 660-nm Protein Assay produces a higher level of protein-to-protein variation (37%) than other assays, such as the BCA Protein Assay, the simpler single-reagent format and broader substance compatibility make the Pierce 660-nm assay more convenient for many routine applications. The Pierce 660-nm Protein Assay can be performed in either a test tube or microplate format.
How the Pierce 660-nm Assay detects protein
The Pierce 660-nm Protein Assay is based on the binding of a proprietary dye-metal complex to protein in acidic conditions that causes a shift in the dye's absorption maximum, which is measured at 660 nm. The dye-metal complex is reddish-brown and changes to green upon protein binding. The color change is produced by deprotonation of the dye at low pH facilitated by interactions with positively charged amino acid groups in proteins. Therefore, the dye interacts mainly with basic residues in proteins, such as histidine, arginine, and lysine and to a lesser extent tyrosine, tryptophan, and phenylalanine.
The color produced in the assay is stable and increases in proportion to a broad range of increasing protein concentrations, even in the presence of detergents and reducing agents that would be incompatible with Bradford and BCA Protein Assays.
Related products
Pierce 660-nm Protein Assay Kit (includes protein standards)
Ionic Detergent Compatibility Reagent for Pierce 660-nm Protein Assay Reagent
Pro-Detect™ Rapid MBP Assay Kit Thermo Scientific™
The Thermo Scientific Pro-Detect Rapid MBP Assay Kit is a single-use, dipstick lateral flow kit for quick, easy detection of maltose binding protein (MBP)-tagged proteins in protein expression and purification systems. This lateral flow assay uses membrane-based strips coated with dye-bound conjugated antibodies specific to the MBP tag to provide a visual, color readout for detection within ten minutes. This assay can be used to detect MBP-tagged proteins directly from cell culture media (supernatant) or cell lysates without any special instrumentation or handling.
Features of the Pro-Detect Rapid assay kits:
• Easy to adopt—lateral flow strips can be used early in and throughout expression to monitor and confirm expression of desired protein-tagged target
• Simple, fast method—simply dilute sample using provided diluent and dip in the lateral flow strip for visual results within 5-10 minutes
• Sensitive—detect protein tags in supernatant or cell lysate samples down to low µg/mL concentrations
• Specific—assay does not cross-react with common lysis and protein preparation reagents
• Reliable—results are similar to other antibody immunoassays, including western blot and ELISA
• Long shelf life—stable for at least one year at 4°C
Each Pro-Detect Rapid assay is performed by simply adding a properly diluted tissue culture supernatant, lysate, or purified protein sample to a test tube or microplate well and dipping in a lateral flow strip. Gold-conjugated detection antibodies embedded in the strip form complexes with the tagged protein in the sample. These complexes travel the length of the strip membrane and bind at the test and control lines. Results are displayed as red bands.
Features of the Pro-Detect Rapid assay kits:
• Easy to adopt—lateral flow strips can be used early in and throughout expression to monitor and confirm expression of desired protein-tagged target
• Simple, fast method—simply dilute sample using provided diluent and dip in the lateral flow strip for visual results within 5-10 minutes
• Sensitive—detect protein tags in supernatant or cell lysate samples down to low µg/mL concentrations
• Specific—assay does not cross-react with common lysis and protein preparation reagents
• Reliable—results are similar to other antibody immunoassays, including western blot and ELISA
• Long shelf life—stable for at least one year at 4°C
Each Pro-Detect Rapid assay is performed by simply adding a properly diluted tissue culture supernatant, lysate, or purified protein sample to a test tube or microplate well and dipping in a lateral flow strip. Gold-conjugated detection antibodies embedded in the strip form complexes with the tagged protein in the sample. These complexes travel the length of the strip membrane and bind at the test and control lines. Results are displayed as red bands.
Qubit™ 4 Protein BR Starter Kit Invitrogen™
The Qubit 4 Protein BR Starter Kit includes:
• Qubit 4 Fluorometer (Cat. No. Q33226)
• Qubit Assay Tubes (500 tubes) (Cat. No. Q32856)
• WiFi Dongle (Cat. No. A26774)
• Qubit Protein BR Assay Kit (100 assays) (Cat. No. A50668)
The Qubit 4 Fluorometer with WiFi is designed to accurately measure DNA, RNA, and protein quantity. The Qubit 4 Fluorometer and Qubit Protein BR Assay Kit work together to accurately quantify protein within a broad range of 0.1–20 mg/mL. The assay is tolerant of many common contaminants, such as detergents, reducing reagents (DTT, β-mercaptoethanol), salts, and nucleic acids.
For all Qubit assays, the concentration or quality of the target molecule in the sample is reported by a fluorescent dye that emits a signal only when bound to the target, which minimizes the effects of contaminants on the result. The easy-to-use touch-screen menus of the Qubit Fluorometer make it easy to select and run the assays you need, with results displayed in just a few seconds.
Fast, simple, and accurate measurement of protein concentration
The Qubit Protein BR Assay Kit provides a quick, easy, and reliable method for determining the protein concentration of purified proteins, lysates, serum, plasma, or complex protein mixtures. The assay can deliver accurate protein quantification across a broad range (0.1–20 mg/mL), enabling most samples to be used neat (undiluted) and eliminating the guess work and dilution steps that accompany traditional protein quantitation methods.
The Qubit Protein BR Assay Kit is a fluorescence-based assay intended for use with the Qubit 4 Fluorometer. The assay is not recommended for other Qubit models. The kit comes with two calibration standards, Protein BR Assay Buffer, and Protein BR Assay Reagent. Simply add 150–160 µL of Protein BR Assay Buffer to 10 or 20 µL of your sample, followed by 30 µL of Protein BR Assay Reagent. Incubate for 10 min at room temperature. Then insert sample into a Qubit 4 instrument and read the concentration.
Save samples and bench space
The thoughtfully designed Qubit 4 Fluorometer requires samples of only 1–20 µL. It is intended for use at room temperature and occupies only a small amount of bench space. The fluorometer features advanced optics and data analysis algorithms, and comes equipped with a WiFi dongle, a USB thumb drive, and cable for data transfer to Excel™ software and for software downloads, as well as a universal power supply, four plug adapters, and electromagnetic CE certification.
The Qubit 4 Fluorometer enables maximum flexibility for data export using WiFi, USB drive, or USB cable direct to computer. Additionally, all data can be saved in the Qubit cloud application, including direct transfer from the instrument via WiFi.
Learn more about the Qubit fluorometers ›
• Qubit 4 Fluorometer (Cat. No. Q33226)
• Qubit Assay Tubes (500 tubes) (Cat. No. Q32856)
• WiFi Dongle (Cat. No. A26774)
• Qubit Protein BR Assay Kit (100 assays) (Cat. No. A50668)
The Qubit 4 Fluorometer with WiFi is designed to accurately measure DNA, RNA, and protein quantity. The Qubit 4 Fluorometer and Qubit Protein BR Assay Kit work together to accurately quantify protein within a broad range of 0.1–20 mg/mL. The assay is tolerant of many common contaminants, such as detergents, reducing reagents (DTT, β-mercaptoethanol), salts, and nucleic acids.
For all Qubit assays, the concentration or quality of the target molecule in the sample is reported by a fluorescent dye that emits a signal only when bound to the target, which minimizes the effects of contaminants on the result. The easy-to-use touch-screen menus of the Qubit Fluorometer make it easy to select and run the assays you need, with results displayed in just a few seconds.
Fast, simple, and accurate measurement of protein concentration
The Qubit Protein BR Assay Kit provides a quick, easy, and reliable method for determining the protein concentration of purified proteins, lysates, serum, plasma, or complex protein mixtures. The assay can deliver accurate protein quantification across a broad range (0.1–20 mg/mL), enabling most samples to be used neat (undiluted) and eliminating the guess work and dilution steps that accompany traditional protein quantitation methods.
The Qubit Protein BR Assay Kit is a fluorescence-based assay intended for use with the Qubit 4 Fluorometer. The assay is not recommended for other Qubit models. The kit comes with two calibration standards, Protein BR Assay Buffer, and Protein BR Assay Reagent. Simply add 150–160 µL of Protein BR Assay Buffer to 10 or 20 µL of your sample, followed by 30 µL of Protein BR Assay Reagent. Incubate for 10 min at room temperature. Then insert sample into a Qubit 4 instrument and read the concentration.
Save samples and bench space
The thoughtfully designed Qubit 4 Fluorometer requires samples of only 1–20 µL. It is intended for use at room temperature and occupies only a small amount of bench space. The fluorometer features advanced optics and data analysis algorithms, and comes equipped with a WiFi dongle, a USB thumb drive, and cable for data transfer to Excel™ software and for software downloads, as well as a universal power supply, four plug adapters, and electromagnetic CE certification.
The Qubit 4 Fluorometer enables maximum flexibility for data export using WiFi, USB drive, or USB cable direct to computer. Additionally, all data can be saved in the Qubit cloud application, including direct transfer from the instrument via WiFi.
Learn more about the Qubit fluorometers ›
Pierce™ 660nm Protein Assay Kit Thermo Scientific™
The Pierce 660-nm Protein Assay is a ready-to-use, detergent- and reducing agent-compatible assay reagent to quickly measure total protein concentration compared to a protein standard. The assay is more linear than Coomassie-based Bradford assays and compatible with higher concentrations of most detergents, reducing agents, and other commonly used reagents.
See all available protein assays ›
Features of the 660-nm Protein Assay Kit:
• Compatibility—works with a greater range of detergents and reducing agents than other dye-based assays
• Ready-to-use—single reagent with a simple mix-and-read protocol; no working reagent to prepare
• Linearity—produces standard curves that are more linear than with the Bradford assay method
• Assay format—assay may be performed in test tubes or microplates
• Sample volume—requires only 10 µL for microplate or 100 µL for the test tube procedures
• Storage—room temperature storage
The accessory Ionic Detergent Compatibility Reagent (IDCR) provides for even broader detergent compatibility, making this one of the only protein assays that is suitable for samples containing Laemmli SDS sample buffer with bromophenol blue. Although the Pierce 660-nm Protein Assay produces a higher level of protein-to-protein variation (37%) than other assays, such as the BCA Protein Assay, the simpler single-reagent format and broader substance compatibility make the Pierce 660-nm assay more convenient for many routine applications. The Pierce 660-nm Protein Assay can be performed in either a test tube or microplate format.
How the Pierce 660-nm Assay detects protein
The Pierce 660-nm Protein Assay is based on the binding of a proprietary dye-metal complex to protein in acidic conditions that causes a shift in the dye's absorption maximum, which is measured at 660 nm. The dye-metal complex is reddish-brown and changes to green upon protein binding. The color change is produced by deprotonation of the dye at low pH facilitated by interactions with positively charged amino acid groups in proteins. Therefore, the dye interacts mainly with basic residues in proteins, such as histidine, arginine, and lysine and to a lesser extent tyrosine, tryptophan, and phenylalanine.
The color produced in the assay is stable and increases in proportion to a broad range of increasing protein concentrations, even in the presence of detergents and reducing agents that would be incompatible with Bradford and BCA protein assays.
Related products
Pierce 660-nm Protein Assay Reagent
Ionic Detergent Compatibility Reagent for Pierce 660-nm Protein Assay Reagent
See all available protein assays ›
Features of the 660-nm Protein Assay Kit:
• Compatibility—works with a greater range of detergents and reducing agents than other dye-based assays
• Ready-to-use—single reagent with a simple mix-and-read protocol; no working reagent to prepare
• Linearity—produces standard curves that are more linear than with the Bradford assay method
• Assay format—assay may be performed in test tubes or microplates
• Sample volume—requires only 10 µL for microplate or 100 µL for the test tube procedures
• Storage—room temperature storage
The accessory Ionic Detergent Compatibility Reagent (IDCR) provides for even broader detergent compatibility, making this one of the only protein assays that is suitable for samples containing Laemmli SDS sample buffer with bromophenol blue. Although the Pierce 660-nm Protein Assay produces a higher level of protein-to-protein variation (37%) than other assays, such as the BCA Protein Assay, the simpler single-reagent format and broader substance compatibility make the Pierce 660-nm assay more convenient for many routine applications. The Pierce 660-nm Protein Assay can be performed in either a test tube or microplate format.
How the Pierce 660-nm Assay detects protein
The Pierce 660-nm Protein Assay is based on the binding of a proprietary dye-metal complex to protein in acidic conditions that causes a shift in the dye's absorption maximum, which is measured at 660 nm. The dye-metal complex is reddish-brown and changes to green upon protein binding. The color change is produced by deprotonation of the dye at low pH facilitated by interactions with positively charged amino acid groups in proteins. Therefore, the dye interacts mainly with basic residues in proteins, such as histidine, arginine, and lysine and to a lesser extent tyrosine, tryptophan, and phenylalanine.
The color produced in the assay is stable and increases in proportion to a broad range of increasing protein concentrations, even in the presence of detergents and reducing agents that would be incompatible with Bradford and BCA protein assays.
Related products
Pierce 660-nm Protein Assay Reagent
Ionic Detergent Compatibility Reagent for Pierce 660-nm Protein Assay Reagent
NanoOrange™ Protein Quantitation Kit, 200-2,000 assays Invitrogen™
The NanoOrange Protein Quantitation Kit contains a very sensitive and easy assay for protein quantitation, with detection as low as 10 ng/mL of protein in solution. This fluorescent dye is suitable for use with spectrofluorometers and microplate readers. For detection of lipoproteins or proteins in a complex lipid environment, check out our CBQCA Protein Quantitation Kit (C-6667).
Pro-Detect™ Rapid Human Fc Assay Kit Thermo Scientific™
The Thermo Scientific Pro-Detect Rapid Human Fc Assay Kit is a single-use, dipstick lateral flow kit for quick, easy detection of human Fc-tagged proteins in protein expression and purification systems. This lateral flow assay uses membrane-based strips coated with dye-bound conjugated antibodies specific to the human Fc tag to provide a visual, color readout for detection within ten minutes. This assay can be used to detect human Fc-tagged proteins directly from cell culture media (supernatant) or cell lysates without any special instrumentation or handling.
Features of the Pro-Detect Rapid assay kits:
• Easy to adopt—lateral flow strips can be used early in and throughout expression to monitor and confirm expression of desired protein-tagged target
• Simple, fast method—simply dilute sample using provided diluent and dip in the lateral flow strip for visual results within 5-10 minutes
• Sensitive—detect protein tags in supernatant or cell lysate samples down to low µg/mL concentrations
• Specific—assay does not cross-react with common lysis and protein preparation reagents
• Reliable—results are similar to other antibody immunoassays, including western blot and ELISA
• Long shelf life—stable for at least one year at 4°C
Each Pro-Detect Rapid assay is performed by simply adding a properly diluted tissue culture supernatant, lysate, or purified protein sample to a test tube or microplate well and dipping in a lateral flow strip. Gold-conjugated detection antibodies embedded in the strip form complexes with the tagged protein in the sample. These complexes travel the length of the strip membrane and bind at the test and control lines. Results are displayed as red bands.
Features of the Pro-Detect Rapid assay kits:
• Easy to adopt—lateral flow strips can be used early in and throughout expression to monitor and confirm expression of desired protein-tagged target
• Simple, fast method—simply dilute sample using provided diluent and dip in the lateral flow strip for visual results within 5-10 minutes
• Sensitive—detect protein tags in supernatant or cell lysate samples down to low µg/mL concentrations
• Specific—assay does not cross-react with common lysis and protein preparation reagents
• Reliable—results are similar to other antibody immunoassays, including western blot and ELISA
• Long shelf life—stable for at least one year at 4°C
Each Pro-Detect Rapid assay is performed by simply adding a properly diluted tissue culture supernatant, lysate, or purified protein sample to a test tube or microplate well and dipping in a lateral flow strip. Gold-conjugated detection antibodies embedded in the strip form complexes with the tagged protein in the sample. These complexes travel the length of the strip membrane and bind at the test and control lines. Results are displayed as red bands.
Pierce™ Modified Lowry Protein Assay Kit Thermo Scientific™
The Pierce Modified Lowry Protein Assay is a stable form of a traditional, two-component, folin phenol- and copper-based reagent system to measure total protein concentration compared to a protein standard.
See all available protein assays ›
Features of Modified Lowry Protein Assay include:
• Popular method—widely cited in protein research literature
• Colorimetric—measure with a standard spectrophotometer or plate reader at 750 nm
• Stability—uses a modified cupric sulfate-tartrate reagent that is stable at room temperature
• Assay range—exhibits good linearity in the range 1 to 1500 µg/mL (tested with BSA protein)
The Modified Lowry Protein Assay Kit combines a stabilized formulation of the original Lowry reagents and the essential Folin-Ciocalteu Phenol reagent in a complete kit for accurately determining protein concentration in a variety of samples types. Although newer protein assay methods provide greater speed and convenience, the Lowry method remains a popular, accurate, and useful option for many applications.
See all available protein assays ›
Features of Modified Lowry Protein Assay include:
• Popular method—widely cited in protein research literature
• Colorimetric—measure with a standard spectrophotometer or plate reader at 750 nm
• Stability—uses a modified cupric sulfate-tartrate reagent that is stable at room temperature
• Assay range—exhibits good linearity in the range 1 to 1500 µg/mL (tested with BSA protein)
The Modified Lowry Protein Assay Kit combines a stabilized formulation of the original Lowry reagents and the essential Folin-Ciocalteu Phenol reagent in a complete kit for accurately determining protein concentration in a variety of samples types. Although newer protein assay methods provide greater speed and convenience, the Lowry method remains a popular, accurate, and useful option for many applications.
Ionic Detergent Compatibility Reagent for Pierce™ 660nm Protein Assay Reagent Thermo Scientific™
The Ionic Detergent Compatibility Reagent is intended for use with the 660 nm Protein Assay reagent (catalog numbers 22662 and 22660). It provides for even broader detergent compatibility, making the 660 nm Protein Assay one of the only protein assays suitable for samples containing Laemmli SDS sample buffer with bromophenol blue. The Ionic Detergent Compatibility Reagent completely dissolves by thorough mixing and does affect the sensitivity of the assay.
Related Products
Pierce™ 660nm Protein Assay Reagent
Pierce™ 660nm Protein Assay Kit
Related Products
Pierce™ 660nm Protein Assay Reagent
Pierce™ 660nm Protein Assay Kit
Qubit™ Protein BR Assay Kit Invitrogen™
The Qubit Protein BR Assay Kit is a quick and accurate fluorescence-based protein quantitation assay. This assay is designed for use with the Qubit 4 Fluorometer and can quantitate samples within a broad range of 0.1–20 mg/mL. The assay is compatible with many common contaminants, such as detergents, reducing reagents (DTT, β-mercaptoethanol), salts, and nucleic acids.
Key features of the Qubit Protein BR Assay Kit include:
• Broad dynamic range—0.1–20 mg/mL, measure samples without dilution
• Two-point standard curve—eliminate time-consuming standard preparation
• Automatic concentration determination—receive protein concentration instantaneously; no need for offline calculations
• Compatible—accurate in the presence of reducing reagents, detergents, and other common buffer components
The Qubit Protein BR Assay Kit provides a quick, easy, and reliable method for determining protein concentration of purified proteins, lysates, serum, plasma, or complex protein mixtures. The assay can deliver accurate protein quantitation across a broad range (0.1–20 mg/mL), enabling most samples to be used neat (undiluted) and eliminating the guess work and dilution steps that accompany traditional protein quantitation methods.
The Qubit Protein BR Assay Kit is a fluorescence-based assay intended for use with the Qubit 4 Fluorometer. The assay is not recommended for other Qubit models. The kit comes with two calibration standards, Protein BR Assay Buffer, and Protein BR Assay Reagent. Simply add 150–160 µL of Protein BR Assay Buffer to 10 or 20 µL of your sample, followed by 30 µL of Protein BR Assay Reagent. Incubate for 10 min at room temperature. Then insert the sample into a Qubit 4 instrument and read the concentration.
View all Qubit protein assays ›
Note: 500-µL thin-walled clear PCR tubes (Cat. No. Q32856) are required but not included in the assay kit.
Key features of the Qubit Protein BR Assay Kit include:
• Broad dynamic range—0.1–20 mg/mL, measure samples without dilution
• Two-point standard curve—eliminate time-consuming standard preparation
• Automatic concentration determination—receive protein concentration instantaneously; no need for offline calculations
• Compatible—accurate in the presence of reducing reagents, detergents, and other common buffer components
The Qubit Protein BR Assay Kit provides a quick, easy, and reliable method for determining protein concentration of purified proteins, lysates, serum, plasma, or complex protein mixtures. The assay can deliver accurate protein quantitation across a broad range (0.1–20 mg/mL), enabling most samples to be used neat (undiluted) and eliminating the guess work and dilution steps that accompany traditional protein quantitation methods.
The Qubit Protein BR Assay Kit is a fluorescence-based assay intended for use with the Qubit 4 Fluorometer. The assay is not recommended for other Qubit models. The kit comes with two calibration standards, Protein BR Assay Buffer, and Protein BR Assay Reagent. Simply add 150–160 µL of Protein BR Assay Buffer to 10 or 20 µL of your sample, followed by 30 µL of Protein BR Assay Reagent. Incubate for 10 min at room temperature. Then insert the sample into a Qubit 4 instrument and read the concentration.
View all Qubit protein assays ›
Note: 500-µL thin-walled clear PCR tubes (Cat. No. Q32856) are required but not included in the assay kit.
Micro BCA™ Reagent A (MA) Thermo Scientific™
The Pierce Micro BCA Reagent A (MA) is a unique alkaline tartrate-carbonate buffer. This product is sufficient for 3,200 microplate assays when mixed with Reagents MB and MC.
Compare all available BCA protein assays ›
Related products
Micro BCA Protein Assay Kit
Micro BCA Reagent B (MB)
Micro BCA Reagent C (MC)
Compare all available BCA protein assays ›
Related products
Micro BCA Protein Assay Kit
Micro BCA Reagent B (MB)
Micro BCA Reagent C (MC)
Pro-Detect™ Rapid Rabbit Fc Assay Kit Thermo Scientific™
The Thermo Scientific Pro-Detect Rapid Rabbit Fc Assay Kit is a single-use, dipstick lateral flow kit for quick, easy detection of rabbit Fc-tagged proteins in protein expression and purification systems. This lateral flow assay uses membrane-based strips coated with dye-bound conjugated antibodies specific to the rabbit Fc tag to provide a visual, color readout for detection within ten minutes. This assay can be used to detect rabbit Fc-tagged proteins directly from cell culture media (supernatant) or cell lysates without any special instrumentation or handling.
Features of the Pro-Detect Rapid assay kits:
• Easy to adopt—lateral flow strips can be used early in and throughout expression to monitor and confirm expression of desired protein-tagged target
• Simple, fast method—simply dilute sample using provided diluent and dip in the lateral flow strip for visual results within 5-10 minutes
• Sensitive—detect protein tags in supernatant or cell lysate samples down to low µg/mL concentrations
• Specific—assay does not cross-react with common lysis and protein preparation reagents
• Reliable—results are similar to other antibody immunoassays, including western blot and ELISA
• Long shelf life—stable for at least one year at 4°C
Each Pro-Detect Rapid assay is performed by simply adding a properly diluted tissue culture supernatant, lysate, or purified protein sample to a test tube or microplate well and dipping in a lateral flow strip. Gold-conjugated detection antibodies embedded in the strip form complexes with the tagged protein in the sample. These complexes travel the length of the strip membrane and bind at the test and control lines. Results are displayed as red bands.
Features of the Pro-Detect Rapid assay kits:
• Easy to adopt—lateral flow strips can be used early in and throughout expression to monitor and confirm expression of desired protein-tagged target
• Simple, fast method—simply dilute sample using provided diluent and dip in the lateral flow strip for visual results within 5-10 minutes
• Sensitive—detect protein tags in supernatant or cell lysate samples down to low µg/mL concentrations
• Specific—assay does not cross-react with common lysis and protein preparation reagents
• Reliable—results are similar to other antibody immunoassays, including western blot and ELISA
• Long shelf life—stable for at least one year at 4°C
Each Pro-Detect Rapid assay is performed by simply adding a properly diluted tissue culture supernatant, lysate, or purified protein sample to a test tube or microplate well and dipping in a lateral flow strip. Gold-conjugated detection antibodies embedded in the strip form complexes with the tagged protein in the sample. These complexes travel the length of the strip membrane and bind at the test and control lines. Results are displayed as red bands.
Pierce™ BCA Protein Assay Reagent A Thermo Scientific™
BCA Protein Assay Reagent A is a component of the Pierce BCA Protein Assay Kit, a two-component, high-precision, detergent-compatible assay that is used for total protein concentration determination compared to a protein standard. Pierce BCA reagents provide accurate determination of protein concentration with most sample types encountered in protein research. The Pierce BCA assay can be used to assess yields in whole cell lysates, affinity-column fractions, purified proteins samples, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods, the BCA assay is affected much less by protein compositional differences, providing greater concentration accuracy.
Compare all available BCA protein assays ›
Features of the Pierce BCA Protein Assay Kit (Reagents A and B) include:
• Colorimetric—estimate visually or measure with a standard spectrophotometer or plate reader at 562 nm
• Uniformity—exhibits less protein-to-protein variation than dye-binding methods (Bradford)
• Compatibility—unaffected by typical concentrations of most ionic and non-ionic detergents
• Assay time—30-min incubation; much easier and four times faster than classical Lowry methods
• Assay range—linear working range for BSA of 20 to 2000 µg/mL
• Sensitivity—detect down to 5 µg/mL with the enhanced protocol
How the assay works
The BCA Protein Assay combines the well-known reduction of Cu2+ to Cu1+ by protein in an alkaline medium with the highly sensitive and selective colorimetric detection of the cuprous cation (Cu1+) by bicinchoninic acid (BCA). The first step is the chelation of copper with protein in an alkaline environment to form a light blue complex. In this reaction, known as the biuret reaction, peptides containing three or more amino acid residues form a colored chelate complex with cupric ions in an alkaline environment containing sodium potassium tartrate.
In the second step of the color development reaction, BCA reacts with the reduced (cuprous) cation that was formed in step one. The intense purple-colored reaction product results from the chelation of two molecules of BCA with one cuprous ion. The BCA/copper complex is water soluble and exhibits a strong linear absorbance at 562 nm with increasing protein concentrations. The complex is approximately 100 times more sensitive (lower limit of detection) than the pale blue color of the first reaction.
The reaction is strongly influenced by four amino acid residues (cysteine, cystine, tyrosine, and tryptophan) in the amino acid sequence of the protein. However, unlike Coomassie dye-binding methods, the universal peptide backbone also contributes to color formation, helping to minimize variability caused by protein compositional differences.
Related products
Pierce BCA Protein Assay Kit
Pierce BCA Protein Assay Reagent B
Pierce BCA Solid
Compare all available BCA protein assays ›
Features of the Pierce BCA Protein Assay Kit (Reagents A and B) include:
• Colorimetric—estimate visually or measure with a standard spectrophotometer or plate reader at 562 nm
• Uniformity—exhibits less protein-to-protein variation than dye-binding methods (Bradford)
• Compatibility—unaffected by typical concentrations of most ionic and non-ionic detergents
• Assay time—30-min incubation; much easier and four times faster than classical Lowry methods
• Assay range—linear working range for BSA of 20 to 2000 µg/mL
• Sensitivity—detect down to 5 µg/mL with the enhanced protocol
How the assay works
The BCA Protein Assay combines the well-known reduction of Cu2+ to Cu1+ by protein in an alkaline medium with the highly sensitive and selective colorimetric detection of the cuprous cation (Cu1+) by bicinchoninic acid (BCA). The first step is the chelation of copper with protein in an alkaline environment to form a light blue complex. In this reaction, known as the biuret reaction, peptides containing three or more amino acid residues form a colored chelate complex with cupric ions in an alkaline environment containing sodium potassium tartrate.
In the second step of the color development reaction, BCA reacts with the reduced (cuprous) cation that was formed in step one. The intense purple-colored reaction product results from the chelation of two molecules of BCA with one cuprous ion. The BCA/copper complex is water soluble and exhibits a strong linear absorbance at 562 nm with increasing protein concentrations. The complex is approximately 100 times more sensitive (lower limit of detection) than the pale blue color of the first reaction.
The reaction is strongly influenced by four amino acid residues (cysteine, cystine, tyrosine, and tryptophan) in the amino acid sequence of the protein. However, unlike Coomassie dye-binding methods, the universal peptide backbone also contributes to color formation, helping to minimize variability caused by protein compositional differences.
Related products
Pierce BCA Protein Assay Kit
Pierce BCA Protein Assay Reagent B
Pierce BCA Solid
Pierce™ Rapid Gold BCA Protein Assay Kit Thermo Scientific™
The Pierce Rapid Gold BCA Protein Assay Kit is a fast, two-component, high-precision, detergent-compatible assay optimized to determine total protein concentration. It uses the same copper-chelating technology as the well-known traditional Pierce BCA Protein Assay and provides comparable accuracy but with a 5-min, room temperature incubation and measured at 480 nm. This improvement eliminates the need to wait or expose the samples to elevated temperatures for a fast time to results.
Compare all available BCA protein assays ›
Features of the Rapid Gold BCA Protein Assay Kit include:
• Absorbance—colorimetric assay, with optimal absorbance at 480 nm
• Uniformity—exhibits similar protein-to-protein variation as traditional BCA Protein Assay and less protein-to-protein variation than dye-binding protein assay methods (Bradford)
• Compatibility—unaffected by typical concentrations of most ionic and non-ionic detergents
• Assay time—5-min, room temperature reaction
• Assay range—linear working range for BSA of 20 to 2000 µg/mL
The traditional BCA Protein Assay is a widely used and accepted protein assay, trusted for its highly accurate protein concentration determination and compatibility with most sample types encountered in protein research. However, in order to develop the samples in this traditional protocol, one must either heat the reaction at 37°C for 30 minutes or allow room temperature development for two hours. The Rapid Gold BCA Protein Assay maintains the key characteristics of the traditional BCA assay but allows a fast time and room temperature incubation equal to dye-binding methods. The Rapid Gold BCA assay can be used to assess yields in whole cell lysates and affinity-column fractions, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods and similar to the traditional BCA assay, the Rapid Gold BCA assay is affected much less by protein compositional differences, providing low protein-to-protein variation.
How the Rapid Gold BCA Protein Assay detects protein
The Rapid Gold BCA Protein Assay uses the same copper reduction method as the traditional BCA Protein Assay with a unique copper chelator. The assay combines the well-known reduction of Cu2+ to Cu1+ by protein in an alkaline medium with the highly sensitive and selective colorimetric detection of the cuprous cation (Cu1+) by the proprietary chelator. The first step is the chelation of copper with protein in an alkaline environment to form a light green complex. In this reaction, known as the biuret reaction, peptides containing three or more amino acid residues form a colored chelate complex with cupric ions in an alkaline environment containing sodium potassium tartrate.
In the second step of the color development reaction, the Rapid Gold BCA chelator reacts with the reduced (cuprous) cation that was formed in step one. The intense gold-colored reaction product results from the chelation of two molecules of BCA with one cuprous ion. The BCA/copper complex is water soluble and exhibits a strong linear absorbance at 480 nm with increasing protein concentrations. The complex is approximately 100 times more sensitive (lower limit of detection) than the pale green color of the first reaction.
The reaction that leads to the color formation is strongly influenced by four amino acid residues (cysteine, cystine, tyrosine, and tryptophan) in the amino acid sequence of the protein. However, unlike the Coomassie dye-binding methods, the universal peptide backbone also contributes to color formation, helping to minimize variability caused by protein compositional differences.
Compatibility of the Rapid Gold BCA Protein Assay
Certain substances are known to interfere with the Rapid Gold BCA Protein Assay, including those with reducing potential, chelating agents, and strong acids or bases. Because they are known to interfere with protein estimation at even minute concentrations, avoid the following substances as components of the sample buffer:
• Ascorbic acid
• EGTA
• Iron
• Impure sucrose
• Catecholamines
• Impure glycerol
• Lipids
• Tryptophan
• Creatinine
• Hydrogen peroxide
• Melibiose
• Tyrosine
• Cysteine
• Hydrazides
• Phenol Red
• Uric acid
Compare all available BCA protein assays ›
Features of the Rapid Gold BCA Protein Assay Kit include:
• Absorbance—colorimetric assay, with optimal absorbance at 480 nm
• Uniformity—exhibits similar protein-to-protein variation as traditional BCA Protein Assay and less protein-to-protein variation than dye-binding protein assay methods (Bradford)
• Compatibility—unaffected by typical concentrations of most ionic and non-ionic detergents
• Assay time—5-min, room temperature reaction
• Assay range—linear working range for BSA of 20 to 2000 µg/mL
The traditional BCA Protein Assay is a widely used and accepted protein assay, trusted for its highly accurate protein concentration determination and compatibility with most sample types encountered in protein research. However, in order to develop the samples in this traditional protocol, one must either heat the reaction at 37°C for 30 minutes or allow room temperature development for two hours. The Rapid Gold BCA Protein Assay maintains the key characteristics of the traditional BCA assay but allows a fast time and room temperature incubation equal to dye-binding methods. The Rapid Gold BCA assay can be used to assess yields in whole cell lysates and affinity-column fractions, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods and similar to the traditional BCA assay, the Rapid Gold BCA assay is affected much less by protein compositional differences, providing low protein-to-protein variation.
How the Rapid Gold BCA Protein Assay detects protein
The Rapid Gold BCA Protein Assay uses the same copper reduction method as the traditional BCA Protein Assay with a unique copper chelator. The assay combines the well-known reduction of Cu2+ to Cu1+ by protein in an alkaline medium with the highly sensitive and selective colorimetric detection of the cuprous cation (Cu1+) by the proprietary chelator. The first step is the chelation of copper with protein in an alkaline environment to form a light green complex. In this reaction, known as the biuret reaction, peptides containing three or more amino acid residues form a colored chelate complex with cupric ions in an alkaline environment containing sodium potassium tartrate.
In the second step of the color development reaction, the Rapid Gold BCA chelator reacts with the reduced (cuprous) cation that was formed in step one. The intense gold-colored reaction product results from the chelation of two molecules of BCA with one cuprous ion. The BCA/copper complex is water soluble and exhibits a strong linear absorbance at 480 nm with increasing protein concentrations. The complex is approximately 100 times more sensitive (lower limit of detection) than the pale green color of the first reaction.
The reaction that leads to the color formation is strongly influenced by four amino acid residues (cysteine, cystine, tyrosine, and tryptophan) in the amino acid sequence of the protein. However, unlike the Coomassie dye-binding methods, the universal peptide backbone also contributes to color formation, helping to minimize variability caused by protein compositional differences.
Compatibility of the Rapid Gold BCA Protein Assay
Certain substances are known to interfere with the Rapid Gold BCA Protein Assay, including those with reducing potential, chelating agents, and strong acids or bases. Because they are known to interfere with protein estimation at even minute concentrations, avoid the following substances as components of the sample buffer:
• Ascorbic acid
• EGTA
• Iron
• Impure sucrose
• Catecholamines
• Impure glycerol
• Lipids
• Tryptophan
• Creatinine
• Hydrogen peroxide
• Melibiose
• Tyrosine
• Cysteine
• Hydrazides
• Phenol Red
• Uric acid
Pierce™ Bovine Serum Albumin Standard Pre-Diluted Set Thermo Scientific™
Thermo Scientific Pierce BSA Protein Assay Standards are high-quality reference samples for generating accurate standard curves and calibration controls in total protein assays.
Features of BSA Protein Assay Standards:
• Convenient—complete set containing seven ready-to-use dilutions
• Universal—recognized as the industry standard for protein quantitation in colorimetric protein assays
• Pure and stable—supplied in ultrapure 0.9% saline solution with 0.05% sodium azide
These bovine serum albumin (BSA) solutions are protein concentration reference standards for use in BCA, Bradford and other protein assay protocols. BSA is the universally accepted reference protein for total protein quantitation. The set of standards is prepared from a stock solution that is calibrated by direct comparison to purified BSA (Fraction V) from the National Institute of Standards and Technology (NIST).
Applications:
• Protein assay quantitation standard (BCA Protein Assay, Coomassie-Bradford Assay, etc.)
• Protein recovery control for desalting and other column procedures
• General calibration of spectrophotometer UV-lamp (absorbance at 280nm)
Selection of a protein standard is potentially the greatest source of error in any protein assay. The best choice for a standard is a purified, known concentration of the most abundant protein contained in the samples being tested. Often, a highly purified, known concentration of the protein of interest is not available or it is too expensive to use as the standard, or the sample itself is a mixture of many proteins (e.g., cell lysate). In such cases, the best standard is one that will produce a normal (i.e., average) color response curve with the selected protein assay method and is readily available to any researcher. BSA is such a protein, and the Pierce Albumin Standards are the most convenient source of ready-to-use BSA standard.
For greatest accuracy in estimating total protein concentration in unknown samples, it is essential to include a standard curve each time the assay is performed. This is particularly true for the protein assay methods that produce non-linear standard curves. Deciding on the number of standards and replicates used to define the standard curve depends upon the degree of non-linearity in the standard curve and the degree of accuracy required. In general, fewer points are needed to construct a standard curve if the color response is linear. Typically, standard curves are constructed using at least two replicates for each point on the curve.
Related Products
Pierce™ Bovine Serum Albumin Standard Ampules, 2 mg/mL
Features of BSA Protein Assay Standards:
• Convenient—complete set containing seven ready-to-use dilutions
• Universal—recognized as the industry standard for protein quantitation in colorimetric protein assays
• Pure and stable—supplied in ultrapure 0.9% saline solution with 0.05% sodium azide
These bovine serum albumin (BSA) solutions are protein concentration reference standards for use in BCA, Bradford and other protein assay protocols. BSA is the universally accepted reference protein for total protein quantitation. The set of standards is prepared from a stock solution that is calibrated by direct comparison to purified BSA (Fraction V) from the National Institute of Standards and Technology (NIST).
Applications:
• Protein assay quantitation standard (BCA Protein Assay, Coomassie-Bradford Assay, etc.)
• Protein recovery control for desalting and other column procedures
• General calibration of spectrophotometer UV-lamp (absorbance at 280nm)
Selection of a protein standard is potentially the greatest source of error in any protein assay. The best choice for a standard is a purified, known concentration of the most abundant protein contained in the samples being tested. Often, a highly purified, known concentration of the protein of interest is not available or it is too expensive to use as the standard, or the sample itself is a mixture of many proteins (e.g., cell lysate). In such cases, the best standard is one that will produce a normal (i.e., average) color response curve with the selected protein assay method and is readily available to any researcher. BSA is such a protein, and the Pierce Albumin Standards are the most convenient source of ready-to-use BSA standard.
For greatest accuracy in estimating total protein concentration in unknown samples, it is essential to include a standard curve each time the assay is performed. This is particularly true for the protein assay methods that produce non-linear standard curves. Deciding on the number of standards and replicates used to define the standard curve depends upon the degree of non-linearity in the standard curve and the degree of accuracy required. In general, fewer points are needed to construct a standard curve if the color response is linear. Typically, standard curves are constructed using at least two replicates for each point on the curve.
Related Products
Pierce™ Bovine Serum Albumin Standard Ampules, 2 mg/mL
Micro BCA™ Protein Assay Kit Thermo Scientific™
The Pierce Micro BCA Protein Assay Kit is a three-component version of our BCA reagents, optimized to measure total protein concentration of dilute protein solutions (0.5 to 20 µg/mL).
Compare all available BCA protein assays ›
Features of the Micro BCA Protein Assay Kit include:
• Sensitivity—accurately detect down to 0.5 µg/mL (2 µg/mL in microplate format)
• Working range—linear working range for BSA of 0.5 to 20 µg/mL
• Colorimetric—measure with a standard spectrophotometer or plate reader at 562 nm
• Compatibility—unaffected by typical concentrations of most ionic and non-ionic detergents
• Stability—kit is stable at room temperature; prepared working reagent stable for up to 24 hours
The Micro BCA Protein Assay Kit is a specialized version of the popular Pierce BCA Protein Assay for determining the protein concentration of dilute samples. Mixing together the three Micro BCA reagents results in a working solution that is sufficiently concentrated to measure protein when mixed with an equal volume of sample. The result is an assay for accurately measuring 0.5 to 20 µg/mL protein solutions. The assay is exceptionally linear and exhibits very low levels of protein-to-protein variability.
Related products
Micro BCA Reagent A (MA)
Micro BCA Reagent B (MB)
Micro BCA Reagent C (MC)
Ampule Breakers
Compare all available BCA protein assays ›
Features of the Micro BCA Protein Assay Kit include:
• Sensitivity—accurately detect down to 0.5 µg/mL (2 µg/mL in microplate format)
• Working range—linear working range for BSA of 0.5 to 20 µg/mL
• Colorimetric—measure with a standard spectrophotometer or plate reader at 562 nm
• Compatibility—unaffected by typical concentrations of most ionic and non-ionic detergents
• Stability—kit is stable at room temperature; prepared working reagent stable for up to 24 hours
The Micro BCA Protein Assay Kit is a specialized version of the popular Pierce BCA Protein Assay for determining the protein concentration of dilute samples. Mixing together the three Micro BCA reagents results in a working solution that is sufficiently concentrated to measure protein when mixed with an equal volume of sample. The result is an assay for accurately measuring 0.5 to 20 µg/mL protein solutions. The assay is exceptionally linear and exhibits very low levels of protein-to-protein variability.
Related products
Micro BCA Reagent A (MA)
Micro BCA Reagent B (MB)
Micro BCA Reagent C (MC)
Ampule Breakers
