Shop All Cell Lysis Detergents

Tween™ 20 Surfact-Amps™ Detergent Solution Thermo Scientific™

Thermo Scientific Tween-20 Surfact-Amps Detergent Solution is a highly-purified Tween™ 20 detergent stabilized as a 10% solution (w/v) and packaged under nitrogen in glass ampules or non-leaching HDPE bottles, ensuring it's stability and eliminating the accumulation of peroxides and degradation products.

Features of Tween-20 Surfact-Amps Detergent Solution:

Tween 20—a popular nonionic detergent for use in ELISA, Western blotting and other immunoassay PBS or TBS wash buffers
Accurate—precise 10% detergent solution in ultrapure water
Easy-to-use—solution is simple to dispense and dilute for use
Exceptionally pure—less than 1.0µeq/mL peroxides and carbonyls
Stable—packaged under inert nitrogen gas in glass ampules or HDPE bottles

Properties of Tween 20:
• Molecular Weight: 1228g
• Detergent Class: Nonionic polyoxyethylene surfactant
• Aggregation Number: Unknown
• Micelle Molecular Weight: Unknown
• Critical Micelle Concentration (CMC): 0.06 mM (0.0074%, w/v)
• Cloud Point: 95°C
• Dialyzable: No

Specifications for Tween 20 Surfact-Amps Detergent Solution:
• Visual: Clear, light yellow liquid, free of particulates.
• Concentration: 10.0±1.0%
• Oxidants: ≤1.0µeq/mL
• Carbonyls: ≤1.0µeq/mL
• Suspended Solids: Residue present must not exceed Residue Reference.

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Lysis Buffer, FS Applied Biosystems™

Lysis Buffer, FS is designed specifically to lyse microbial organisms from food and environmental samples and prepare the microbial DNA for subsequent downstream analysis by real-time PCR.

This lysis buffer can be used in conjunction with Proteinase K, FS in the Pathatrix® Auto Instrument or RapidFinder™ Direct Lysis workflows.

When used as a part of the Pathatrix® Auto Instrument workflow, one bottle of Lysis Buffer, FS is sufficient for 700 reactions.

When used as a part of the RapidFinder™ Direct Lysis workflow, one bottle of Lysis Buffer, FS is sufficient for 100 reactions.

Brij™-35 Surfact-Amps™ Detergent Solution Thermo Scientific™

This Thermo Scientific™ formulation of Brij™ 35 is a stabilized 30% (w/v) solution of Brij-35 detergent for use in various protein methods. Brij™ 35 is a nonionic polyoxyethylene surfactant that is most frequently used as a component of cell lysis buffers or a surfactant in various HPLC applications.

Properties of Brij 35 Detergent:

• Molecular Weight: 1225g
• Detergent Class: Nonionic
• Aggregation Number: 40
• Micelle Molecular Weight: 49,000g
• Critical Micelle Concentration (CMC): 0.09 mM (0.011%, w/v)
• Cloud Point: >100°C
• Dialyzable: No

Features of this Thermo Scientific detergent solution:

Brij 35—a nonionic detergent for use in various protein methods
Accurate—30% detergent solution in ultrapure water
Easy-to-use—solutions are simple to dispense and dilute for use

Specifications of this Thermo Scientific Brij 35, 30% Solution:

• Visual: Clear, colorless viscous liquid, free of foreign material.
• Concentration: Concentration: 27.0 to 30.0%

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AAV-MAX Lysis Buffer, prototype Gibco™

Gibco AAV-MAX Lysis Buffer, prototype, is a ready-to-use cell lysis reagent for the extraction of adeno-associated virus (AAV) particles from producer HEK293 cells. Cell lysis is detergent-based, eliminating the need for mechanical cell disruption, providing a milder and easier alternative when isolating AAV particles from cell cultures. The buffer is supplied as a 10X solution that can be directly added to HEK293 AAV production cultures to induce cell lysis.

Gibco AAV-MAX Lysis Buffer, prototype, is a Polysorbate 20-based formulation and does not contain Triton™ X-100.

Tween™ 80 Surfact-Amps™ Detergent Solution Thermo Scientific™

Thermo Scientific Tween-80 Surfact-Amps Detergent Solution is a highly-purified Tween™ 80 detergent stabilized as a 10% solution (w/v) and packaged under nitrogen in glass ampules or non-leaching HDPE bottles, ensuring it's stability and eliminating the accumulation of peroxides and degradation products.

Features of Tween-80 Surfact-Amps Detergent Solution:

Tween 80—a popular nonionic detergent for use in ELISA, Western blotting and other immunoassay PBS or TBS wash buffers
Accurate—precise 10% detergent solution in ultrapure water
Easy-to-use—solution is simple to dispense and dilute for use
Exceptionally pure—less than 1.0µeq/mL peroxides and carbonyls
Stable—packaged under inert nitrogen gas in glass ampules or HDPE bottles

Properties of Tween 80:
• Molecular Weight: 1310g
• Detergent Class: Nonionic polyoxyethylene surfactant
• Aggregation Number: 60
• Micelle Molecular Weight: 76,000g
• Critical Micelle Concentration (CMC): 0.012 mM (0.0016%, w/v)
• Cloud Point: Unknown
• Dialyzable: No

Specifications for Tween 80 Surfact-Amps Detergent Solution:
• Visual: Slightly hazy to cloudy, colorless solution, free of particulates; may form layers.
• Concentration: 10.0±1.0%
• Oxidants: ≤1.0µeq/mL
• Carbonyls: ≤1.0µeq/mL
• Suspended Solids: Residue present must not exceed Residue Reference.

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CHAPS Detergent (3-((3-cholamidopropyl) dimethylammonio)-1-propanesulfonate) Thermo Scientific™

Thermo Scientific CHAPS is a zwitterionic detergent that is especially well suited for protecting the native state of proteins.

CHAPS is sulfobetaine derivative of cholic acid. This zwitterionic detergent is useful for membrane protein solubilization when it is important to maintain protein activity. CHAPS detergent is soluble over a wide range of pH (2 to 12) and is easily removed from solution by dialysis because it has a high critical micelle concentration (CMC).

Features of this buffer:

• Versatile zwitterionic detergent
• Provides mild but effective lysis of cultured mammalian cells
• Nondenaturing and generally does not inactivate protein functions
• Can be removed from solution by dialysis
• Commonly used for isoelectric focusing (IEF) and 2D electrophoresis

Applications:

• Solubilizing the opiate receptor from rat brain cell membranes, while retaining the reversible opiate binding property of the receptor 1
• Purifying microsomal signal peptidase as a complex 2
• Solubilizing dopamine D 2,3, muscarinic 4, and opiate receptors 5 from brain tissue

CHAPS has been successfully used to solubilize intrinsic membrane proteins and receptors and maintain the functional capability of the protein of interest. CHAPS has also been used in combination with nonionic detergents such as NP-40 for nondenaturing gel electrophoresis applications. CHAPS has largely replaced NP-40 in isoelectric focusing (IEF) applications, where it prevents streaking in certain pH ranges. The neutral charge of CHAPS and its disaggregating properties are believed to be responsible for the improvement.

Properties of CHAPS Detergent:

• Chemical Name: 3-[(3-Cholamidopropyl) dimethylammonio]-1-propanesulfonate
• Molecular Weight: 614.88g
• Detergent Class: Zwitterionic
• Aggregation Number: 10
• Micelle Molecular Weight: 6149g
• Critical Micelle Concentration (CMC): 8 to 10 mM (0.4920 to 0.6150%, w/v)
• Cloud Point: ≥100°C
• Dialyzable: Yes

Specifications for CHAPS:

• Visual: White free-flowing powder, free of foreign matter.
• Solubility: A 10% (aq) solution is clear, colorless.
• Identity: The I.R. scan must show only peaks characteristic for the structure and functional groups of the compound being tested.
• Purity: (TLC) One major spot. A small, secondary spot is acceptable.

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Triton™ X-100 Surfact-Amps™ Detergent Solution Thermo Scientific™

Thermo Scientific Triton X-100 Surfact-Amps Detergent Solution is a highly-purified Triton™ X-100 detergent stabilized as a 10% solution (w/v) in variable sizes packaged under nitrogen in 10 mL glass ampules or three sizes of non-leaching HDPE bottles, ensuring their stability and eliminating the accumulation of peroxides and degradation products.

Features of Triton X-100 Surfact-Amps Detergent Solution:

Triton X-100—a nonionic detergent for use in various protein methods
Accurate—precise 10% detergent solution in ultrapure water
Easy-to-use—solution is simple to dispense and dilute for use
Exceptionally pure—less than 1.0µeq/mL peroxides and carbonyls
Stable—packaged under inert nitrogen gas in glass ampules or HDPE bottles

Specifications for Triton X-100 Surfact-Amps Detergent Solution:
• Visual: Clear to slightly hazy, colorless solution, free of particulate matter.
• Concentration: 10.0±1.0%
• Oxidants: ≤1.0µeq/mL
• Carbonyls: ≤1.0µeq/mL
• Suspended Solids: Residue present must not exceed Residue Reference.

Properties of Triton X-100:
• Molecular Weight: 647g
• Detergent Class: Nonionic polyoxyethylene surfactant
• Aggregation Number: 140
• Micelle Molecular Weight: 90,000g
• Critical Micelle Concentration (CMC): 0.24 mM (0.155%, w/v)
• Cloud Point: 64°C
• Dialyzable: No

Triton X-100 is most frequently used as a component of cell lysis buffers or other solutions intended to extract and solubilize proteins.

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Octyl-beta-Glucoside Detergent Thermo Scientific™

Thermo Scientific Octyl-beta-Glucoside is a nonionic detergent that has been widely used for membrane protein solubilization.

Features of Octyl-beta-Glucoside:

• A low-molecular weight, nonionic detergent
• Effective for membrane protein solubilization
• Less stable and less commonly used than octylthioglucoside (OTG)
• Can be removed from solution by dialysis
• Optically clear; low absorbance at 280nm

Properties of N-Octyl-β-D-glucoside:
• Alternative Names: Octyl-beta-glucoside, OG, Octyl-beta-glucopyranoside, Octyl-beta-D-glucopyranoside
• Chemical Name: n-Octyl-β-D-glucoside
• Molecular Weight: 292.37g
• Detergent Class: Nonionic
• Aggregation Number: 27
• Micelle Molecular Weight: 8000g
• Critical Micelle Concentration (CMC): 23 to 25 mM (0.6716 to 0.7300%, w/v)
• Cloud Point: >100°C
• Dialyzable: Yes

Specifications for Octyl-beta-Glucoside (Part No. 28309, 28310):
• Visual: White, free-flowing powder, free of foreign material.
• Solubility: A 20% (D.I. water) solution must be clear, colorless, with no particulates.
• Absorbance (400nm) of a 20% solution: <0.025
• Absorbance (280nm) of a 20% solution: <0.300
• Specific Rotation (20°C, 1%): -29.0 to -32.0°C
• Identity: The IR scan must show only peaks characteristic for the structure and functional groups of the compound being tested.

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NP-40 Surfact-Amps™ Detergent Solution Thermo Scientific™

Thermo Scientific Surfact-Amps NP-40 Detergent Solution is highly-purified NP-40 detergent stabilized as a 10% solution in your choice of either 10 mL glass ampules or two sizes of HDPE-plastic bottles.

Features of Surfact-Amps NP-40 Detergent Solution:

NP-40—a nonionic detergent for use in various protein methods
Accurate—precise 10% detergent solutions in ultrapure water
Easy-to-use—solutions are simple to dispense and dilute for use
Exceptionally pure—less than 1.0µeq/mL peroxides and carbonyls
Stable—packaged under inert nitrogen gas in HDPE bottles

This is an easy-to-use 10% (w/v) solution of purified NP-40 detergent for use in routine and high-demand protein research methods and molecular biology techniques. NP-40 is a nonionic polyoxyethylene surfactant that is most frequently used as a component of cell lysis buffers or other solutions intended to extract and solubilize proteins. Surfact-Amps Detergent Solutions (10% w/v) provide unsurpassed purity, quality and stability. Unlike neat detergents, which are extremely viscous, Surfact-Amps 10% Solutions are easy to pipette and accurately dispense. The surfactant solutions are carefully prepared and packaged under nitrogen in glass ampules or non-leaching HDPE bottles, ensuring their stability and eliminating the accumulation of peroxides and degradation products.

Properties of NP-40 Detergent:
• Molecular Weight: 617g
• Detergent Class: Nonionic
• Aggregation Number: 149
• Micelle Molecular Weight: 90,000g
• Critical Micelle Concentration (CMC): 0.29 mM (0.0179%, w/v)
• Cloud Point: 80°C
• Dialyzable: No

Specifications for NP-40 Surfact-Amps Detergent Solution:
• Visual: Clear, colorless solution, free of particulate matter.
• Concentration: 10.0±1.0%
• Oxidants: ≤1.0µeq/mL
• Carbonyls: ≤1.0µeq/mL
• Suspended Solids: Residue present must not exceed Residue Reference.

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Sodium Dodecyl Sulfate (SDS), Lauryl Thermo Scientific™

Thermo Scientific Sodium Dodecyl Sulfate (Lauryl) is standard-grade SDS detergent for use in protein polyacrylamide gel electrophoresis (PAGE).

Features of Sodium Dodecyl Sulfate (Lauryl):

• Popular anionic detergent for a variety of protein methods
• Especially useful for denaturing polyacrylamide gel electrophoresis (SDS-PAGE)
• Common component of cell lysis buffers

This lauryl-grade sodium dodecyl sulfate (SDS) is a popular anionic detergent for routine protein electrophoresis and cell lysis methods. The formulation is a mixture of several different alkyl sulfate chain lengths (C10 to C18).

Properties of SDS (values for pure C12):
• Molecular Weight: 288.5g
• Detergent Class: Ionic (anionic)
• Aggregation Number: 62
• Micelle Molecular Weight: 18,000g
• Critical Micelle Concentration (CMC): 6 to 8 mM (0.1728 to 0.2304%, w/v)
• Cloud Point: >100°C
• Dialyzable: No

Specifications for Sodium Dodecyl Sulfate:
• Visual: White powder, free of foreign material.
• Solubility: 10% (aq, w/v) solution must be clear, colorless to slightly yellow.
• Chain length: C12 >60%; C14 = 20 to 35%; C16 <10%; C10 and C18 <1% each

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Brij™-35, 30% Solution Thermo Scientific™

This Thermo Scientific™ formulation of Brij™ 35 is a stabilized 30% (w/v) solution of Brij-35 detergent for use in various protein methods. Brij™ 35 is a nonionic polyoxyethylene surfactant that is most frequently used as a component of cell lysis buffers or a surfactant in various HPLC applications.

Properties of Brij 35 Detergent:

• Molecular Weight: 1225g
• Detergent Class: Nonionic
• Aggregation Number: 40
• Micelle Molecular Weight: 49,000g
• Critical Micelle Concentration (CMC): 0.09 mM (0.011%, w/v)
• Cloud Point: >100°C
• Dialyzable: No

Features of this Thermo Scientific detergent solution:

Brij 35—a nonionic detergent for use in various protein methods
Accurate—30% detergent solution in ultrapure water
Easy-to-use—solutions are simple to dispense and dilute for use

Specifications of this Thermo Scientific Brij 35, 30% Solution:

• Visual: Clear, colorless viscous liquid, free of foreign material.
• Concentration: Concentration: 27.0 to 30.0%

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Triton X-100 (1%) Invitrogen™

This reagent, a component of the Fetal Hemoglobin (HbF) test, comes as a 1% solution that can be diluted in phosphate-buffered saline (PBS) containing bovine serum albumin (BSA) and sodium azide to create a 0.1% working solution.

n-Dodecyl-beta-Maltoside Detergent Thermo Scientific™

Thermo Scientific n-Dodecyl-β-D-Maltoside is especially useful for solubilizing membrane proteins to preserve their activity.

The water soluble nonionic detergent is most often used for the isolation of hydrophobic membrane proteins. Multiple studies have shown that n-Dodecyl-β-D-maltoside is a gentle detergent that is often able to preserve protein activity better than many commonly used detergents, including NP-40, CHAPS and Octyl-β-glucoside.

Features of n-Dodecyl-β-D-Maltoside:

• Lipid-like nonionic detergent
• Especially useful for isolating and stabilizing hydrophobic membrane proteins
• Preserves activity of membrane protein better than most of the detergents
• High-purity compound with low UV absorptivity

Like most detergents, n-Dodecyl-β-D-maltoside has dual hydrophobic/hydrophilic properties that facilitate lipid displacement and provide a lipid-like environment for membrane proteins. Studies suggest that the ability of these surfactants to preserve membrane protein structure stems in part from the reduced disruption of lipid:protein interactions where some of the natural lipid associations are maintained. While no detergent or set of conditions is optimal for all studies on membrane proteins, n-Dodecyl-β-D-maltoside has unique characteristics that have made it especially helpful in certain protein methods.

Properties of n-Dodecyl-β-D-Maltoside:
• Chemical Name: n-Dodecyl-beta-D-maltoside
• Molecular Weight: 510.6g
• Detergent Class: Nonionic
• Aggregation Number: 98 (average), 70 to 140 range
• Micelle Molecular Weight: 50,000g
• Critical Micelle Concentration (CMC): 0.17 mM (0.009%, w/v) in water ; 0.12 mM (0.006%, w/v) in 0.2M NaCl
• Cloud Point: Unknown
• Dialyzable: No

Specifications for n-Dodecyl-beta-maltoside (Part No. 89902, 89903):
• Formula: C24H46O11
• Molecular Weight: 510.6g
• Purity (by HPLC): ≥99%
• Absorbance (1% Detergent Solution): 340nm <0.02; 280nm <0.04; 260nm <0.06; 225nm <0.1
• pH (1% Solution): 5 to 8
• Solubility (in water at 0 to 5°C): ≥20%
• Conductivity (10% Solution) : <40mS

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Sodium Dodecyl Sulfate (SDS), C12 Thermo Scientific™

Thermo Scientific Sodium Dodecyl Sulfate (C12) is a highly purified form of SDS that is especially suited for protein experiments requiring tightly controlled solubility parameters.

Features of Sodium Dodecyl Sulfate (C12):

• Popular anionic detergent for protein electrophoresis and solubilization
• Component of many cell lysis buffer formulations
• Specially purified formulation is >98% C12 alkyl sulfate
• Contains low levels of hexadecyl sulfate (C16), which inhibits protein renaturation

Sodium dodecyl sulfate (SDS) is an alkyl sulfate, anionic detergent used frequently in protein electrophoresis and protein solubilization methods. It is a component of many cell lysis buffer formulations, including RIPA buffer. Typical SDS or lauryl sulfate preparations are mixtures of several different chain lengths (C10 to C18). However, protein renaturation and other specialized methods require very specific surfactant and buffer conditions. This particular formulation of SDS is highly purified to eliminate nearly all secondary chain lengths besides C12.

Properties of Sodium Dodecyl Sulfate, (pure C12):
• Molecular Weight: 288.5g
• Detergent Class: Ionic (anionic)
• Aggregation Number: 62
• Micelle Molecular Weight: 18,000g
• Critical Micelle Concentration (CMC): 6 to 8 mM (0.1728 to 0.2304%, w/v)
• Cloud Point: >100°C
• Dialyzable: No

Specifications for Sodium Dodecyl Sulfate (SDS), C12 (Part No. 28312):
• Visual: White powder, free of foreign material.
• Solubility: 10% (aq, w/v) solution must be clear, colorless and free of particulates.
• Chain length: C12 ≥98.0% if C16 ≤0.8%
• Report C10, C12, C14, C16, C18 concentrations.

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Triton™ X-114 Surfact-Amps™ Detergent Solution Thermo Scientific™

Thermo Scientific Triton X-114 Surfact-Amps Detergent Solution is a stabilized 10% (w/v) solution of highly-purified Triton™ X-114 detergent, supplied in glass ampules and packaged under nitrogen inert gas, ensuring their stability and eliminating the accumulation of peroxides and degradation products.

Features of Triton X-114 Surfact-Amps Detergent Solution:

Triton X-114—a nonionic detergent for use in various routine protein and molecular biology techniques
Accurate—precise 10% detergent solution in ultrapure water
Easy-to-use—solution is simple to dispense and dilute for use
Exceptionally pure—less than 1.0µeq/mL peroxides and carbonyls
Stable— packaged in glass ampules under inert nitrogen gas

Specifications for Triton X-114 Surfact-Amps Detergent Solution:
• Visual: Slightly hazy to cloudy, colorless solution, free of particulates; may form layers.
• Concentration: 10.0±1.0%
• Oxidants: ≤1.0µeq/mL
• Carbonyls: ≤1.0µeq/mL
• Suspended Solids: Residue present must not exceed Residue Reference.

Properties of Triton X-114:
• Molecular Weight: 537g
• Detergent Class: Nonionic polyoxyethylene surfactant
• Aggregation Number: unknown
• Micelle Molecular Weight: unknown
• Critical Micelle Concentration (CMC): 0.21 mM (0.0113%, w/v)
• Cloud Point: 23°C
• Dialyzable: No

Triton X-114 is most frequently used as a component of cell lysis buffers. In certain conditions, this detergent phase-separates in aqueous solutions, a property that has been used to separate hydrophilic and hydrophobic (membrane) proteins.

For added safety when opening glass ampules, consider using our Ampule Breakers, which are disposable safety devices that protect the fingers when breaking open a glass ampule.

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