Shop All Magnetic Affinity Separation Particles

Pierce™ High Capacity Ni-IMAC MagBeads, EDTA compatible Thermo Scientific

Pierce high-capacity EDTA-compatible Ni-IMAC MagBeads are designed for purification of over-expressed His-tagged proteins from cell culture media. The beads have high binding capacity (up to 80 mg of protein per mL of settled beads) and can tolerate the high EDTA and reducing agent concentrations that are used to inhibit metalloproteases and stabilize sensitive intracellular proteins.

Features of the high-capacity EDTA-compatible Ni-IMAC MagBeads include:
• Chelator stable—stable in buffer containing 20 mM EDTA and DTT
• Compatible with purification of overexpressed, secreted proteins in cell culture media (e.g., Expi293, ExpiCHO, and ExpiSf expression systems)
• Exceptional high protein binding capacity—up to 80 mg/mL of settled beads
• High-performance—non-aggregating, superparamagnetic microparticles provide exceptional performance with manual and automated HTS applications (e.g., Thermo Scientific KingFisher instruments)

Pierce high-capacity EDTA-compatible Ni-IMAC MagBeads consist of crosslinked agarose beads embedded with magnetite. The density of the ligand on the magnetic agarose bead results in a binding capacity similar to or better than traditional agarose resins with the added benefits of having magnetic properties and being EDTA compatible. The MagBeads are a valuable tool for small-scale purification of multiple His-tagged proteins and for scouting expression and purification conditions to be used in larger-scale purifications.

Pierce high-capacity EDTA-compatible Ni-IMAC MagBeads are optimized for use with high-throughput magnetic platforms, such as the KingFisher 96 and KingFisher Flex magnetic particle processors, but the beads also compatible with simple benchtop purification applications using an appropriate magnetic stand. Automated instruments, however, are especially useful for higher throughput purification and screening of purification conditions.

Pierce™ Anti-HA Magnetic Beads Thermo Scientific™

Thermo Scientific Pierce Anti-HA Magnetic Beads are affinity particles for immunoprecipitation of recombinant HA-tagged proteins expressed in bacterial or mammalian cells or in vitro systems, using manual or robotic magnetic separators.

Features of Anti-HA Magnetic Beads:

Specific—highly specific anti-HA monoclonal antibody (clone 2-2.2.14) enables high yield and high purity immunoprecipitation
Convenient and fast—product instructions provide an easy-to-follow, optimized protocol for immunoprecipitation in approximately one hour
Low non-specific binding—stable, pre-blocked beads and specific antibody minimize off-target binding for HA-tag IP or co-IP experiments
Versatile—beads are compatible with manual and automated workflows (e.g., Thermo Scientific KingFisher Instruments)

The blocked magnetic bead surface is coated with anti-HA antibody, a highly specific mouse IgG1 monoclonal antibody that recognizes the HA-epitope tag (YPYDVPDYA) derived from the human influenza hemagglutinin (HA) protein. The Pierce Anti-HA Magnetic Beads can be used manually with a magnetic stand, as well as with automated platforms, such as the Thermo Scientific KingFisher Instruments, for high-throughput workflows.

Specifications:
Product Details:
Pierce Anti-HA Magnetic Beads are convenient for the immunoprecipitation (IP) of recombinant HA-tagged proteins and the co-immunoprecipitation (co-IP) of their interacting proteins. The beads are incubated with a cell lysate containing HA-tagged protein and the fusion protein is captured. The beads are subsequently washed and then the target proteins are eluted using 0.1M glycine, pH 2.0, 50 mM NaOH, or SDS-PAGE sample buffer. If gentler elution conditions are desired, 2 mg/mL Pierce HA peptide can be used. The protocol has been optimized for each of these conditions. Anti-HA antibody can be used to detect HA-tagged protein by Western blot analysis.

Pathatrix™ Auto Cartridge Applied Biosystems™

The proprietary patented magnetic separation technology with which the Pathatrix® Auto Instrument performs pathogen enrichment and purification occurs within plastic disposable vessels loaded into a Pathatrix® Auto Cartridge. Each Pathatrix Auto Cartridge can be loaded and run independently of the others, and each Pathatrix Auto Instrument has slots for five cartridges.

Contaminated samples are first added to the consumable sample vessel. Paramagnetic beads, surface-modified for pathogen capture, are then added, and the sample vessel and attached elution vessel are loaded into the Pathatrix Auto Cartridge. The Pathatrix Auto Instrument uses magnets housed in the Pathatrix Auto Cartridge to capture the beads, and then efficiently recirculates the sample over the captured beads. Target organisms are retained by the immobilized beads, and after an automated washing and elution step, the isolated organisms are ready for downstream detection. The entire process is automated and takes only about 15 minutes.

Although the Pathatrix® Auto Instrument includes 5 Pathatrix® Auto Cartridges, this additional cartridge may be purchased, either as a replacement, or to provide the added convenience of being able to load a sample while others are running in the instrument.

A Pathatrix® Auto Cartridge Holder (ordered separately) serves as a stable platform for each cartridge during loading and transport.

Pierce™ Protein A Magnetic Beads Thermo Scientific™

Thermo Scientific Pierce Protein A Magnetic Beads are high-capacity and high-throughput affinity particles for antibody purification and immunoprecipitation methods using manual or robotic magnetic separators.

Features of Protein A Magnetic Beads:

High efficiency – equivalent or higher yield of IP target antigens than magnetic beads from other suppliers
Low non-specific binding – stable, pre-blocked beads provide highly purified product (e.g., antigen eluted in IP with antibody is devoid of contaminating protein from complex cell lysate)
Consistent – magnetic beads eliminate resin loss and provide for more efficient separation than traditional IP methods that use only centrifugation
Versatile – beads are compatible with manual and automated workflows (e.g., Thermo Scientific KingFisher Instruments)

Recombinant protein A, covalently attached to a blocked magnetic bead surface, can bind to antibodies from many different species, enabling purification of antibodies from crude extracts. Immunoprecipitation assays performed with protein A coated beads result in high yield of target antigen with very low background. Using our crosslinker chemistry, you can immobilize an antibody onto the magnetic particle and prevent IgG contamination in IP or Co-IP experiments. The Pierce Protein A Magnetic Beads can be used manually with a magnetic stand, as well as with automated platforms, such as the Thermo Scientific KingFisher Instruments, for high-throughput workflows.

Applications:
• IP and Co-IP experiments
• Antibody purification

Thermo Scientific Pierce Protein A Magnetic Beads are used for purifying antibody from serum, cell culture supernatant or ascites, as well as for IP/Co-IP of antigens from cell or tissue extracts. Protein A can bind to antibodies from many different species, including mouse, human, rabbit, pig, dog, and cat. The protocol for the Pierce Protein A beads has been optimized to allow for high recovery and high purity of the isolated antibody or antigen. Antibody or antigen/antibody complex (IP) is first captured on the magnetic beads. The beads are washed and then the target is eluted with low pH elution buffer. IP performance is equivalent to or better than Protein A magnetic beads from other suppliers.

MagnaBind™ Carboxyl Derivatized Beads Thermo Scientific™

Thermo Scientific™ MagnaBind™ Beads provide a convenient method for magnetic separation of antibodies, antigens, lectins, enzymes, nucleic acids and cells using affinity binding. To remove the MagnaBind Beads from the suspension, an external magnetic field is used.

MagnaBind Carboxyl Derivatized Beads are supplied as an aqueous suspension of magnetic iron oxide beads coated with carboxyl groups for covalent coupling of molecules using EDC, a zero-length crosslinker that is amine- and carboxyl-reactive. Additionally, carboxyl derivatized beads have been used to purify plasmid DNA from cells.

Features of MagnaBind Carboxyl Derivatized Beads:

Composition: Silanized iron oxide
Magnetization: 25-35EMU/g
Type of Magnetization: Superparamagnetic (no magnetic memory)
Surface Area: >100m2/g
Bead Size: 1-4µm diameter
Settling Rate: 4% in 30 minutes
Effective Density: 2.5g/mL
Number of Beads: 1 × 108 beads/mg
pH Stability: Aqueous solution, above pH 4.0
Concentration: ~20 mg/mL

Applications:
• Cell sorting using either positive or negative selection
• Protein purification or immunoassays using either direct or indirect methods

MagniSort™ Mouse CD8 Naïve T cell Enrichment Kit Invitrogen™

The MagniSort™ Mouse CD8 Naïve T cell Enrichment Kit is designed for the magnetic separation of CD8 naïve T cells by negative selection. It has been optimized for the isolation of mouse CD8 naïve T cells from spleens or lymphnodes utilizing a biotinylated antibody cocktail and streptavidin-coated magnetic beads. Undesired cells are bound by antibody and then magnetic beads that, when placed in a magnetic field, leave CD8 naïve T cells untouched and free in solution.

The MagniSort™ Mouse CD8 Naïve T cell Enrichment Antibody Cocktail contains the following antibodies:
Anti-Mouse CD4 Biotin
Anti-Mouse CD11b Biotin
Anti-Mouse CD19 Biotin
Anti-Mouse CD24 Biotin
Anti-Mouse CD44 Biotin
Anti-Mouse CD45R (B220) Biotin
Anti-Mouse CD49b (Integrin alpha 2) Biotin
Anti-Mouse Ly-6G (Gr-1) Biotin
Anti-Mouse γδ TCR Biotin
Anti-Mouse TER-119 Biotin

Reported Application
Magnetic Cell Separation

Pathatrix™ Magnetic Capture Plate Applied Biosystems™

The Pathatrix® Magnetic Capture Plate makes it easy to link to endpoint detection by real-time PCR using the DuPont BAX® system. The magnetic rack is used to remove the paramagnetic particles after lysis and prior to detection.

MagnaBind™ Goat Anti-Rabbit IgG Thermo Scientific™

Thermo Scientific™ MagnaBind™ Beads provide a convenient method for magnetic separation of antibodies, antigens, lectins, enzymes, nucleic acids and cells using affinity binding. To remove the MagnaBind Beads from the suspension, an external magnetic field is used.

MagnaBind Goat Anti-Rabbit IgG Beads cab be used to conveniently separate cells of interest from a cell mixture. The monoclonal or polyclonal antibody to the cell surface antigen is pre-incubated with the appropriate magnetic bead that is then incubated with the cell suspension.

Features of MagnaBind Goat Anti-Rabbit IgG Beads:

Composition: Silanized iron oxide
Magnetization: 25-35EMU/g
Type of Magnetization: Superparamagnetic (no magnetic memory)
Surface Area: >100m2/g
Bead Size: 1-4µm diameter
Settling Rate: 4% in 30 minutes
Effective Density: 2.5g/mL
Number of Beads: 1 × 108 beads/mg
pH Stability: Aqueous solution, above pH 4.0
Concentration: ~1 mg/mL

Applications:
• Cell sorting using either positive or negative selection
• Protein purification or immunoassays using either direct or indirect methods

Pierce™ Protein A/G Magnetic Beads Thermo Scientific™

Thermo Scientific Pierce Protein A/G Magnetic Beads are high-performance affinity particles for antibody purification and immunoprecipitation methods using manual or robotic magnetic separators.

Features of Protein A/G Magnetic Beads:

High capacity—nearly four times higher binding capacity than typical magnetic beads from other suppliers, allowing the use of smaller amounts per experiment
Low non-specific binding—stable, pre-blocked beads provide clean purification products (e.g., antigen eluted in IP with antibody is devoid of contaminating proteins from complex IP matrix)
Flexibility—convenience of IgG binding domains of both Protein A and Protein G on one bead
Compatibility—beads are compatible with manual and automated applications (e.g., Thermo Scientific KingFisher Instruments)
Assay consistency—magnetic beads eliminate resin loss and provide for more efficient separation of solutions than traditional IP methods that use only microcentrifuge tubes

These magnetic beads are coated with genetically engineered Pierce Protein A/G, a recombinant fusion protein which combines the IgG binding domains of both Protein A and Protein G. This enables capture of antibodies from a wider range of species and isotypes than either protein alone. Using our crosslinker chemistry, you can immobilize an antibody onto the magnetic particle and prevent IgG contamination in your immunoprecipitated sample. These beads can be used both manually with a magnetic stand as well as with automated platforms such as the Thermo Scientific KingFisher Instruments.

Applications:
• IP and Co-IP experiments (see complete kit)
• Immunoprecipitation for analysis in non-reducing conditions
• Antibody purification

The recombinant Protein A/G that is immobilized onto the Pierce Magnetic Beads is a fusion of the IgG binding domains of both Protein A and Protein G. Protein A/G contains four Fc-binding domains from Protein A and two from Protein G, making it a convenient tool for investigating and purifying immunoglobulins. Thus, Pierce Magnetic Particles are not simply a mixed immobilization of separate Protein A and Protein G polypeptides, nor are they a mixture of Protein A magnetic beads and Protein G magnetic beads.

HisPur™ Ni-NTA Magnetic Beads Thermo Scientific™

Thermo Scientific HisPur Ni-NTA Magnetic Beads are high-capacity nickel-IMAC beads for affinity purification of His-tagged fusion proteins in manual or automated formats.

Features of HisPur Ni-NTA Magnetic Beads:

High capacity—equivalent or higher binding capacity than Ni-NTA magnetic beads from other suppliers
Low nonspecific binding—the bead surface is pre-blocked and the protocol provides optimized buffers for purification
Fast—protocol is completed in 1 hour
Scalable—process microliter to milliliter sample volumes
Versatile—purify proteins using native or denaturing conditions
Reagent compatible—can be used with common cell lysis reagents and a variety of buffer additives
Multiple formats—protein coupling to the beads and downstream applications can be performed both manually and on an automated platform (e.g., Thermo Scientific KingFisher Instruments)

The blocked magnetic bead surface is derivatized with the nitrilotriacetic acid (NTA) chelation moiety and loaded with divalent nickel ions (Ni2+). The immobilized metal affinity chromatography (IMAC) beads provide high binding capacity with very low background. The HisPur Ni-NTA Magnetic Beads can be used both manually with a magnetic stand as well as with automated platforms such as the Thermo Scientific KingFisher Instruments for high-throughput needs.

HisPur Ni-NTA Magnetic Beads are used for small scale affinity purification as well as high-throughput screening of recombinant His-tagged proteins. The polyhistidine tag is the most popular affinity tag and typically consists of six consecutive histidine residues (6xHis). These tagged proteins are overexpressed in a number of different systems, most commonly in bacteria, and purified from cell lysates such as those prepared using B-PER Bacterial Protein Extraction Reagents. Purification of His-tagged proteins is achieved using a NTA chelate charged with nickel that coordinates with the histidine side chains. The NTA chelate contains four metal-binding sites which allow for low metal ion leaching and high binding capacity. The protocol for the HisPur Ni-NTA Magnetic Beads has been optimized to allow for high purity of the isolated His-tagged protein. Performance is equivalent to or better than Ni-NTA magnetic beads from other suppliers.

Pierce™ NHS-Activated Magnetic Beads Thermo Scientific™

Thermo Scientific Pierce NHS-Activated Magnetic Beads enable covalent, amine-based conjugation of proteins to magnetic beads in a simple mix-and-go format for use in custom affinity purification experiments.

Features of NHS-Activated Magnetic Beads:

High capacity—at least four times higher binding capacity than NHS-activated magnetic beads from other suppliers
Easy to use—immobilize in a simple one-step reaction with minimal hands-on time
• Safe—no hazardous chemicals needed (e.g., sodium cyanoborohydride and cyanogen bromide)
Ligand-compatible—use to immobilize with nearly any primary amine-containing compound or affinity ligand
Low non-specific binding—the bead surface is pre-blocked and any nonreacted NHS-ester groups are fully quenched
Protocol-compatible—protein coupling to the beads and downstream applications can be performed manually or by automation (e.g., Thermo Scientific KingFisher Instruments)

The activated magnetic beads contain N-hydroxy-succinimide (NHS) functional groups that react with primary amines forming stable amide linkages. Once they are covalently attached, the immobilized proteins are highly resistant to leaching from the bead surface. When prepared beads are used in experiments, nonspecific binding is negligible because nonreacted NHS-ester groups are thoroughly blocked during the coupling procedure. Pierce NHS-Activated Magnetic beads can be coupled and processed either manually with a magnetic stand or with automated platforms such as the Thermo Scientific KingFisher Instruments.

Applications:
• Immobilization of ligands for the purification of recombinant proteins
• Immobilization of antibodies for immunoprecipitations and co-immunoprecipitations free of antibody contamination in the eluates
• Immobilization of secondary antibody for the affinity purification of antibody subtypes from ascites, serum and cell culture supernatant

Pierce NHS-Activated Magnetic Beads offer a convenient way to conjugate any desired protein to a magnetic bead surface. The process does not require hazardous chemicals or lengthy reaction schemes. The beads are first incubated with protein for 1 to 2 hours in an amine free buffer at pH 7 to 9 to allow for covalent coupling through NHS-ester chemistry. The beads are subsequently washed and then any remaining active NHS-ester groups are quenched. The amine reactive chemistry and subsequent quench are completed in 3 to 4 hours. Coupled protein does not leach from the bead surface. In addition, the beads exhibit very low non-specific binding due to effective quenching and a proprietary blocking agent that coats the surface of the base particle. Following conjugation the prepared magnetic beads are typically used in affinity purification procedures.

Pierce™ Glutathione Magnetic Agarose Beads Thermo Scientific™

Thermo Scientific™ Pierce™ Glutathione Magnetic Agarose Beads provide a fast, convenient method for purification of glutathione-S-transferase (GST) from a bacterial, yeast, or mammalian crude cell lysate. Pierce™ Glutathione Magnetic Agarose Beads are well suited for purifying GST fusion proteins from a soluble protein extract and are ideal for the purification of proteins expressed at low levels from diluted supernatants. The beads can be used in manual applications with a magnetic stand or automated applications with an instrument such as the Thermo Scientific™ KingFisher™ Flex Magnetic Particle Processor. Automated instruments are especially useful for higher throughput purification and screening of purification conditions.

Download the KingFisher™ Duo BindIt™ Software protocol (.bdz) >
Download the KingFisher™ Flex BindIt™ Software protocol (.bdz) >

Features of Glutathione Magnetic Agarose Beads:
• High-performance beads—non-aggregating, magnetite (Fe3O4), superparamagnetic beads provide exceptional uniformity for both manual and automated HTS applications
• Stable affinity ligand—glutathione is covalently immobilized to particles, enabling leach-resistance and clean purification products
• High capacity—binding capacity is sufficient for both routine and demanding purification procedures

The high-performance, magnetite, superparamagnetic particles are validated and optimized for use with high-throughput magnetic platforms, such as the KingFisher™ 96 and KingFisher™ Flex magnetic particle processors, but the beads also enable premium performance for simple benchtop purification applications using an appropriate magnetic stand.

Glutathione S-transferase (GST) is a protein that is commonly added to the end of recombinant proteins to aid in their purification or detection. To make an affinity support, glutathione is immobilized through its central carbon, preserving the essential structure necessary for efficient GST binding. Addition of reduced glutathione in a physiologic buffer is sufficient to competitively displace and recover the GST fusion protein from the immobilized glutathione.

Pierce™ Ni-NTA Magnetic Agarose Beads Thermo Scientific™

Thermo Scientific™ Pierce™ Ni-NTA Magnetic Agarose Beads provide a fast, convenient method for purification of His-tagged recombinant proteins. The beads are incubated with cell lysate containing His-tagged protein and then magnetically separated from the supernatant manually or through automation using an instrument such as the Thermo Scientific™ KingFisher™ Flex Magnetic Particle Processor. Nonspecifically bound protein can be washed away before dissociating bound His-tagged protein with elution buffer. Automated instruments are especially useful for higher throughput purification and screening of purification conditions.

Download the KingFisher™ Duo BindIt™ Software protocol (.bdz) >
Download the KingFisher™ Flex BindIt™ Software protocol (.bdz) >

Pierce Ni-NTA Magnetic Agarose Beads consist of highly crosslinked agarose beads embedded with magnetite and a covalently attached tetradentate nitrilotriacetic acid (NTA) chelator charged with divalent nickel ions. The density of the ligand on the magnetic agarose bead results in a binding capacity similar to or better than traditional agarose resins with the added feature of magnetic handling. Magnetic agarose beads are a valuable tool for small-scale (~1 mg) purification of multiple His-tagged proteins and for scouting expression and purification conditions to be used in larger-scale purifications with agarose chromatography supports.

Features of Ni-NTA Magnetic Agarose Beads:
• High capacity—sufficient for both routine and demanding purification procedures; binds >70 mg of 6xHis-tagged protein per mL of settled resin
• High-performance and reproducible beads—non-aggregating, magnetite (Fe3O4), superparamagnetic beads provide exceptional uniformity for both manual and automated HTS purification applications
• Low non-specific binding—optimized purification protocol results in better tagged-protein purification
• Versatile—purify proteins using native or denaturing conditions
• Compatible—use with Thermo Scientific Cell Lysis reagents and a variety of buffer additives

The high-performance, magnetite-containing, superparamagnetic magnetic agarose resin is validated and optimized for use with high-throughput magnetic platforms, such as the KingFisher 96 and KingFisher Flex magnetic particle processors, but the beads also enable premium performance for simple benchtop purification applications using an appropriate magnetic stand.

Ni-NTA Magnetic Agarose Beads are used for small-scale affinity purification as well as high-throughput screening of recombinant His-tagged proteins. The polyhistidine tag is the most popular affinity tag and typically consists of six consecutive histidine residues (6xHis). Tagged proteins are overexpressed in a number of different systems, most commonly in bacteria, and purified from cell lysates such as those prepared using Thermo Scientific B-PER Bacterial Protein Extraction reagents. Purification of His-tagged proteins is achieved using an NTA chelate charged with nickel that coordinates with the histidine side chains. The NTA chelate contains four metal-binding sites that allow for low metal ion leaching and high binding capacity. The protocol for the Ni-NTA Magnetic Agarose Beads has been optimized to allow for high purity of the isolated His-tagged protein.

MagnaBind™ Goat Anti-Mouse IgG Thermo Scientific™

Thermo Scientific™ MagnaBind™ Beads provide a convenient method for magnetic separation of antibodies, antigens, lectins, enzymes, nucleic acids and cells using affinity binding. To remove the MagnaBind Beads from the suspension, an external magnetic field is used.

MagnaBind Goat Anti-Mouse IgG Beads cab be used to conveniently separate cells of interest from a cell mixture. The monoclonal or polyclonal antibody to the cell surface antigen is pre-incubated with the appropriate magnetic bead that is then incubated with the cell suspension.

Features of MagnaBind Goat Anti-Mouse IgG Beads:

Composition: Silanized iron oxide
Magnetization: 25-35EMU/g
Type of Magnetization: Superparamagnetic (no magnetic memory)
Surface Area: >100m2/g
Bead Size: 1-4µm diameter
Settling Rate: 4% in 30 minutes
Effective Density: 2.5g/mL
Number of Beads: 1 × 108 beads/mg
pH Stability: Aqueous solution, above pH 4.0
Concentration: ~1 mg/mL

Applications:
• Cell sorting using either positive or negative selection
• Protein purification or immunoassays using either direct or indirect methods

MagMax™ Express-96 PCR Well Magnetic Head Applied Biosystems™

This is a magnetic head for the MagMAX™ Express-96 Deep Well Magnetic Particle Processor that is designed for use with PCR plates. Includes one magnetic head. Not compatible with the KingFisher™ Flex 96 Magnetic Particle Processor.
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