Shop All Magnetic Affinity Separation Particles

Pathatrix™ Auto Cartridge Applied Biosystems™

The proprietary patented magnetic separation technology with which the Pathatrix® Auto Instrument performs pathogen enrichment and purification occurs within plastic disposable vessels loaded into a Pathatrix® Auto Cartridge. Each Pathatrix Auto Cartridge can be loaded and run independently of the others, and each Pathatrix Auto Instrument has slots for five cartridges.

Contaminated samples are first added to the consumable sample vessel. Paramagnetic beads, surface-modified for pathogen capture, are then added, and the sample vessel and attached elution vessel are loaded into the Pathatrix Auto Cartridge. The Pathatrix Auto Instrument uses magnets housed in the Pathatrix Auto Cartridge to capture the beads, and then efficiently recirculates the sample over the captured beads. Target organisms are retained by the immobilized beads, and after an automated washing and elution step, the isolated organisms are ready for downstream detection. The entire process is automated and takes only about 15 minutes.

Although the Pathatrix® Auto Instrument includes 5 Pathatrix® Auto Cartridges, this additional cartridge may be purchased, either as a replacement, or to provide the added convenience of being able to load a sample while others are running in the instrument.

A Pathatrix® Auto Cartridge Holder (ordered separately) serves as a stable platform for each cartridge during loading and transport.

DynaMag™-50 Magnet Invitrogen™

The new DynaMag™-50 magnet is optimized for efficient cell isolation using Dynabeads®. The DynaMag™-50 magnet combines a strong magnetic attraction for efficient separations with a flexible and smart ergonomic design that ensures a comfortable working position. A tilting mechanism on the magnet enables you to rest your elbows while pipetting. The magnet can be used for any starting sample in combination with all types of cell-isolation Dynabeads®.
 
→ Optimal working volume: 5–50 ml
→ Magnet holds 2 x 50 ml tubes
→ Excellent control and visibility of your isolations
 
 

NOTE: This magnet replaces the Dynal MPC™-50 magnet

50 mL Tube Magnetic Separator Invitrogen™

This Magnetic Separator is for use with the GeneCatcher™ gDNA 3 - 10 ml Blood Kit. The Magnetic Separator consists of a rack that holds 4 50-ml tubes in alignment with neodymium magnets for the efficient processing of DNA bound to magnetic beads.

GeneCatcher™ Technology is a novel magnetic bead-based technology that provides a method for reliably and efficiently purifying DNA without any centrifugation steps. The GeneCatcher™ gDNA Blood Kits facilitate the efficient and reliable extraction of genomic DNA (gDNA) from samples of human blood, even when the samples have been archived, poorly stored, or collected in the presence of anticoagulants such as EDTA, heparin, or citrate.

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

MagMax™ Express-96 PCR Well Magnetic Head Applied Biosystems™

This is a magnetic head for the MagMAX™ Express-96 Deep Well Magnetic Particle Processor that is designed for use with PCR plates. Includes one magnetic head. Not compatible with the KingFisher™ Flex 96 Magnetic Particle Processor.

24 Deep-Well RB Block Invitrogen™

The 24 Deep Well RB Block is designed for experimental protocols that require volumes exceeding the capacity of conventional shallow well plates. It is completely compatible with the magnetic bead-based technology provided in the GeneCatcher™ gDNA 0.3-1 ml Blood Kit, as well as with the 24-well Magnetic Separator.

The 24 Deep Well RB Block Features:

• Deep, round-bottom wells, with a 10 ml working volume per well
• Polypropylene plastic construction, conforming to the proposed SBS microplate standards
• Guaranteed compatibility with robotic handlers and centrifuge carriers

Dynabeads™ EPEC/VTEC O145 Applied Biosystems™

For the rapid selective concentration of E.coli O145. Method can be performed manually or can be automated by using a BeadRetriever.

This Product Contains: Dynabeads coated with anti-E.coli O145 antibodies

Sensitivity: Sensitivity is very high and enables detection of 1 viable organism in 25g of sample or as few as 100 organisms / ml after pre enrichment

Pierce™ NHS-Activated Magnetic Beads Thermo Scientific™

Thermo Scientific Pierce NHS-Activated Magnetic Beads enable covalent, amine-based conjugation of proteins to magnetic beads in a simple mix-and-go format for use in custom affinity purification experiments.

Features of NHS-Activated Magnetic Beads:

High capacity—at least four times higher binding capacity than NHS-activated magnetic beads from other suppliers
Easy to use—immobilize in a simple one-step reaction with minimal hands-on time
• Safe—no hazardous chemicals needed (e.g., sodium cyanoborohydride and cyanogen bromide)
Ligand-compatible—use to immobilize with nearly any primary amine-containing compound or affinity ligand
Low non-specific binding—the bead surface is pre-blocked and any nonreacted NHS-ester groups are fully quenched
Protocol-compatible—protein coupling to the beads and downstream applications can be performed manually or by automation (e.g., Thermo Scientific KingFisher Instruments)

The activated magnetic beads contain N-hydroxy-succinimide (NHS) functional groups that react with primary amines forming stable amide linkages. Once they are covalently attached, the immobilized proteins are highly resistant to leaching from the bead surface. When prepared beads are used in experiments, nonspecific binding is negligible because nonreacted NHS-ester groups are thoroughly blocked during the coupling procedure. Pierce NHS-Activated Magnetic beads can be coupled and processed either manually with a magnetic stand or with automated platforms such as the Thermo Scientific KingFisher Instruments.

Applications:
• Immobilization of ligands for the purification of recombinant proteins
• Immobilization of antibodies for immunoprecipitations and co-immunoprecipitations free of antibody contamination in the eluates
• Immobilization of secondary antibody for the affinity purification of antibody subtypes from ascites, serum and cell culture supernatant

Pierce NHS-Activated Magnetic Beads offer a convenient way to conjugate any desired protein to a magnetic bead surface. The process does not require hazardous chemicals or lengthy reaction schemes. The beads are first incubated with protein for 1 to 2 hours in an amine free buffer at pH 7 to 9 to allow for covalent coupling through NHS-ester chemistry. The beads are subsequently washed and then any remaining active NHS-ester groups are quenched. The amine reactive chemistry and subsequent quench are completed in 3 to 4 hours. Coupled protein does not leach from the bead surface. In addition, the beads exhibit very low non-specific binding due to effective quenching and a proprietary blocking agent that coats the surface of the base particle. Following conjugation the prepared magnetic beads are typically used in affinity purification procedures.

Pharma Magnetic Stand-96 Applied Biosystems™

The Magnetic Stand-96 is designed for paramagnetic bead precipitation from standard 96-well, U-bottom microplates and 0.2 mL PCR plates with no additional accessories. The stand has a clear polyacryl base in a standard SBS 96-well microplate footprint. The use of 24 extremely strong permanent magnets enables easy and fast magnetic separation in as little as 30 seconds. It sits flat on a lab bench as well as in a microplate adapter on robotic workstations.

MagniSort™ Human NK cell Enrichment Kit Invitrogen™

The MagniSort™ Human NK cell Enrichment Kit is designed for the magnetic separation of NK cells by negative selection. It has been optimized for the isolation of human NK cells from normal human peripheral blood mononuclear cells utilizing a biotinylated antibody cocktail and streptavidin-coated magnetic beads. Undesired cells, including NKT cells, are bound by antibody and then magnetic beads that, when placed in a magnetic field, leave NK cells untouched and free in solution.

The MagniSort™ Human NK cell Enrichment Antibody Cocktail contains the following antibodies:
Anti-Human CD3 Biotin
Anti-Human CD4 Biotin
Anti-Human CD14 Biotin
Anti-Human CD19 Biotin
Anti-Human CD20 Biotin
Anti-Human CD66b Biotin
Anti-Human CD123 Biotin
Anti-Human CD235a Biotin
Anti-Human HLA-DR Biotin

Reported Application
Magnetic Cell Separation

DynaMag™-15 Magnet Invitrogen™

The DynaMag™-15 magnet is optimized for efficient cell isolation using all types of Dynabeads® (range 1–4.5 µm in diameter) in standard 15 mL lab tubes. The DynaMag™-15 magnet holds 4 tubes and combines a strong magnetic attraction for efficient separations with a flexible and smart ergonomic design that ensures a comfortable working position. A tilting mechanism on the magnet enables flexibility while pipetting.

• Optimal working volume: 15 mL
• Holds 4 × 15 mL tubes
• Good control and visibility of your sample

For handling of smaller blood collection tubes, FACS tubes, or similar, see our DynaMag™-5 magnet.

Learn more about Dynabeads® products
• Find Dynabeads® products for a whole range of applications.
• Find other magnets for Dynabeads® separations.

Pathatrix™ Cationic/General Viral Capture Kit Applied Biosystems™

The Pathatrix® Cationic/General Viral Capture Kit, when used with the Pathatrix® Auto Instrument, facilitates the concentration and isolation of viruses from 10-60 ml liquid for downstream detection by PCR, immunoassay or culture.

When used with the Pathatrix® Auto Instrument, the Pathatrix® Cationic/General Viral Capture Kit:
• Improves sensitivity – concentrates target organisms from large sample volumes
• Minimizes false positive and indeterminate rates – isolates target organisms from background flora and PCR inhibitors

Each kit includes one vial of cationic paramagnetic beads and sufficient numbers of sterile, single-use plastic consumables for 50 samples.

MagnaBind™ Goat Anti-Rabbit IgG Thermo Scientific™

Thermo Scientific™ MagnaBind™ Beads provide a convenient method for magnetic separation of antibodies, antigens, lectins, enzymes, nucleic acids and cells using affinity binding. To remove the MagnaBind Beads from the suspension, an external magnetic field is used.

MagnaBind Goat Anti-Rabbit IgG Beads cab be used to conveniently separate cells of interest from a cell mixture. The monoclonal or polyclonal antibody to the cell surface antigen is pre-incubated with the appropriate magnetic bead that is then incubated with the cell suspension.

Features of MagnaBind Goat Anti-Rabbit IgG Beads:

Composition: Silanized iron oxide
Magnetization: 25-35EMU/g
Type of Magnetization: Superparamagnetic (no magnetic memory)
Surface Area: >100m2/g
Bead Size: 1-4µm diameter
Settling Rate: 4% in 30 minutes
Effective Density: 2.5g/mL
Number of Beads: 1 × 108 beads/mg
pH Stability: Aqueous solution, above pH 4.0
Concentration: ~1 mg/mL

Applications:
• Cell sorting using either positive or negative selection
• Protein purification or immunoassays using either direct or indirect methods

Pierce™ Protein A Magnetic Beads Thermo Scientific™

Thermo Scientific Pierce Protein A Magnetic Beads are high-capacity and high-throughput affinity particles for antibody purification and immunoprecipitation methods using manual or robotic magnetic separators.

Features of Protein A Magnetic Beads:

High efficiency – equivalent or higher yield of IP target antigens than magnetic beads from other suppliers
Low non-specific binding – stable, pre-blocked beads provide highly purified product (e.g., antigen eluted in IP with antibody is devoid of contaminating protein from complex cell lysate)
Consistent – magnetic beads eliminate resin loss and provide for more efficient separation than traditional IP methods that use only centrifugation
Versatile – beads are compatible with manual and automated workflows (e.g., Thermo Scientific KingFisher Instruments)

Recombinant protein A, covalently attached to a blocked magnetic bead surface, can bind to antibodies from many different species, enabling purification of antibodies from crude extracts. Immunoprecipitation assays performed with protein A coated beads result in high yield of target antigen with very low background. Using our crosslinker chemistry, you can immobilize an antibody onto the magnetic particle and prevent IgG contamination in IP or Co-IP experiments. The Pierce Protein A Magnetic Beads can be used manually with a magnetic stand, as well as with automated platforms, such as the Thermo Scientific KingFisher Instruments, for high-throughput workflows.

Applications:
• IP and Co-IP experiments
• Antibody purification

Thermo Scientific Pierce Protein A Magnetic Beads are used for purifying antibody from serum, cell culture supernatant or ascites, as well as for IP/Co-IP of antigens from cell or tissue extracts. Protein A can bind to antibodies from many different species, including mouse, human, rabbit, pig, dog, and cat. The protocol for the Pierce Protein A beads has been optimized to allow for high recovery and high purity of the isolated antibody or antigen. Antibody or antigen/antibody complex (IP) is first captured on the magnetic beads. The beads are washed and then the target is eluted with low pH elution buffer. IP performance is equivalent to or better than Protein A magnetic beads from other suppliers.

MagnaBind™ Protein A Beads Thermo Scientific™

Thermo Scientific™ MagnaBind™ Beads provide a convenient method for magnetic separation of antibodies, antigens, lectins, enzymes, nucleic acids and cells using affinity binding. To remove the MagnaBind Beads from the suspension, an external magnetic field is used.

Features of MagnaBind Protein A Beads:

Composition: Silanized iron oxide
Magnetization: 25-35EMU/g
Type of Magnetization: Superparamagnetic (no magnetic memory)
Surface Area: >100m2/g
Bead Size: 1-4µm diameter
Settling Rate: 4% in 30 minutes
Effective Density: 2.5g/mL
Number of Beads: 1 × 108 beads/mg
pH Stability: Aqueous solution, above pH 4.0
Concentration: 5 mg/mL

MagnaBind Protein A Beads are typically used to isolate antibodies from serum and cell culture supernatant and to separate cells of interest from a cell mixture. For antibody purification, the beads are incubated with the antibody solution and then magnetically separated from the supernatant. The antigens and antibodies are then dissociated from the beads using an elution buffer. For cell separation, the monoclonal or polyclonal antibody to the cell surface antigen is incubated with Protein A magnetic beads, magnetically separated, and then incubated with the cell suspension.

Applications:
• Cell sorting using either positive or negative selection
• Protein purification or immunoassays using either direct or indirect methods

MagnaRack™ Magnetic Separation Rack Invitrogen™

The MagnaRack™ consists of a magnetic base station and a removable tube rack. The tube rack can hold up to 24 x 1.5-mL microcentrifuge tubes. The tube rack fits onto the magnetic base station in two different positions associating the row of 12 neodinium magnets with a single row of 12 tubes for simple and easy 'on the magnet' and 'off the magnet' sample processing.
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