Shop All Miscellaneous Immobilized Affinity Ligands

Pierce™ Iminobiotin Agarose Thermo Scientific™

Thermo Scientific Pierce Iminobiotin Agarose provides for unusual affinity purification experiments involving clean-up or removal of streptavidin or avidin protein components.

Features of Iminobiotin Agarose:

• Enable purification or removal of avidin or streptavidin samples
• Crosslinked beaded agarose onto which iminobiotin is covalently immobilized
• Use Iminobiotin Agarose for reversible binding (e.g., non-denaturing purification of avidin)

Pierce Iminobiotin Agarose is beaded agarose support onto which iminobiotin moieties have covalently immobilized. Typical avidin-biotin affinity procedures use immobilized avidin or streptavidin proteins to capture biotin-tagged molecules from a sample. Occasionally, however, the reverse situation arises in which immobilized biotin is needed to capture avidin or streptavidin components from a sample. This iminobiotin resin addresses this special situation.

Immobilized iminobiotin is the guanido analog of biotin. The dissociation constant of the avidin-iminobiotin complex is pH dependent. At pH 9.5 to 11, the avidin-iminobiotin complex will bind tightly. At pH 4, the avidin-iminobiotin complex will dissociate. Because no denaturing agents such as 8M guanidine hydrochloride or 4M urea are required for elution, the avidin conjugate has a better chance of maintaining its activity after purification. Thus, iminobiotin agarose can be used for situations requiring reversible binding and elution of avidin components.

EK-Away™ Resin Invitrogen™

EK-Away™ Resin is specifically designed for removal of EKMax™ or other enterokinase enzymes following cleavage of proteins containing the enterokinase cleavage site (Figure 1). The resin is conjugated with soybean trypsin inhibitor, which has a high affinity and binding capacity for enterokinase. The enzyme catalytic site binds this agarose-based resin for simple batch or column-bound removal of EKMax™ or other enterokinase preparations.

Pierce™ High Capacity Endotoxin Removal Spin Columns, 0.25 mL Thermo Scientific™

Thermo Scientific Pierce High Capacity Endotoxin Removal Resin binds to reduce endotoxin levels in protein samples by ≥99% in 1 hour; with this spin cup format, protein recovery is ≥85%.

Features of High Capacity Endotoxin Removal Resin Spin Columns:

High capacity—bind up to 2,000,000 EU/mL to eliminate >99% of endotoxins
Selective—recover ≥85% of your protein sample
High performance—meets FDA guidelines by reducing final EU concentration to <5 EU/mL
Fast—our new spin cup format enables endotoxin depletion within 1 hour
Clean—single-use spin columns avoid cross contamination of samples
Optimized—spin cups are optimized for different sample volumes

The Pierce High Capacity Endotoxin Removal Resin combines porous cellulose beads and an FDA-approved food preservative, poly(ε-lysine), as an affinity ligand to selectively bind endotoxins. The modified polylysine affinity ligand eliminates the toxicity concerns associated with alternative technologies using polymixin B ligands and sodium deoxycholate buffers.

Requires:
Endotoxin-free water
Applications:

Eliminate endotoxins from protein or antibody samples prior to cell transfection or animal injection

The presence of endotoxins in biologically derived products prepared for research, vaccination, or therapeutic use is a major concern due to the diverse and potentially harmful biological response to these molecules. Endotoxin contamination in proteins purified from bacterial cultures is due to residual lipopolysaccharides (LPS) derived from the outer cell membrane of gram-negative bacteria such as E. coli. Endotoxin-contaminated protein samples transfected into cells or injected into an animal host can initiate a strong immune response resulting in a Systemic Inflammatory Response Syndrome (SIRS) and/or sepsis. Traditional endotoxin removal resins that use polymixin B and sodium deoxycholate to clear endotoxins from a sample also have the negative side effects of neurotoxicity and acute renal tubular necrosis.

Pierce High Capacity Endotoxin Removal Resin is a porous cellulose bead that has been surface-modified with covalently attached polylysine chains that have a high affinity for LPS but lacks the toxicity of other endotoxin-binding molecules. With a 2,000,000-EU/mL binding capacity, endotoxin levels can be reduced by 99% in samples containing initial endotoxin levels of 10,000 EU/mL. Typical protein samples processed with the High Capacity Endotoxin Removal Resin have a final endotoxin concentration below 5 EU/mL. This spin cup format is a fast, single-use method to remove endotoxins from protein samples in as little as 1 hour. The spin cups use a batch format to bind and remove endotoxins while allowing for >85% protein recovery.

One endotoxin unit/mL (EU/mL) equals approximately 0.1ng endotoxin/mL of solution.

More Product Data
Removing endotoxins using a spin-column format
Eliminate endotoxins from protein and antibody samples

Related Products
Pierce™ High Capacity Endotoxin Removal Resin
Pierce™ High Capacity Endotoxin Removal Spin Columns, 0.5 mL
Pierce™ High Capacity Endotoxin Removal Spin Columns, 1 mL

Pierce™ High Capacity Endotoxin Removal Spin Columns, 0.5 mL Thermo Scientific™

Thermo Scientific Pierce High Capacity Endotoxin Removal Resin binds to reduce endotoxin levels in protein samples by ≥99% in 1 hour; with this spin cup format, protein recovery is ≥85%.

Features of High Capacity Endotoxin Removal Resin Spin Columns:

High capacity—bind up to 2,000,000 EU/mL to eliminate >99% of endotoxins
Selective—recover ≥85% of your protein sample
High performance—meets FDA guidelines by reducing final EU concentration to <5 EU/mL
Fast—our new spin cup format enables endotoxin depletion within 1 hour
Clean—single-use spin columns avoid cross contamination of samples
Optimized—spin cups are optimized for different sample volumes

The Pierce High Capacity Endotoxin Removal Resin combines porous cellulose beads and an FDA-approved food preservative, poly(ε-lysine), as an affinity ligand to selectively bind endotoxins. The modified polylysine affinity ligand eliminates the toxicity concerns associated with alternative technologies using polymixin B ligands and sodium deoxycholate buffers.

Requires:
Endotoxin-free water
Applications:

Eliminate endotoxins from protein or antibody samples prior to cell transfection or animal injection

The presence of endotoxins in biologically derived products prepared for research, vaccination, or therapeutic use is a major concern due to the diverse and potentially harmful biological response to these molecules. Endotoxin contamination in proteins purified from bacterial cultures is due to residual lipopolysaccharides (LPS) derived from the outer cell membrane of gram-negative bacteria such as E. coli. Endotoxin-contaminated protein samples transfected into cells or injected into an animal host can initiate a strong immune response resulting in a Systemic Inflammatory Response Syndrome (SIRS) and/or sepsis. Traditional endotoxin removal resins that use polymixin B and sodium deoxycholate to clear endotoxins from a sample also have the negative side effects of neurotoxicity and acute renal tubular necrosis.

Pierce High Capacity Endotoxin Removal Resin is a porous cellulose bead that has been surface-modified with covalently attached polylysine chains that have a high affinity for LPS but lacks the toxicity of other endotoxin-binding molecules. With a 2,000,000-EU/mL binding capacity, endotoxin levels can be reduced by 99% in samples containing initial endotoxin levels of 10,000 EU/mL. Typical protein samples processed with the High Capacity Endotoxin Removal Resin have a final endotoxin concentration below 5 EU/mL. This spin cup format is a fast, single-use method to remove endotoxins from protein samples in as little as 1 hour. The spin cups use a batch format to bind and remove endotoxins while allowing for >85% protein recovery.

One endotoxin unit/mL (EU/mL) equals approximately 0.1ng endotoxin/mL of solution.

More Product Data
Removing endotoxins using a spin-column format
Eliminate endotoxins from protein and antibody samples

Related Products
Pierce™ High Capacity Endotoxin Removal Resin
Pierce™ High Capacity Endotoxin Removal Spin Columns, 0.25 mL

Pierce™ p-Aminophenyl Phosphoryl Choline Agarose Thermo Scientific™

Thermo Scientific Pierce p-Amino Phosphoryl Choline Agarose enables purification of C-reactive protein (CRP).

Features of p-Amino Phosphoryl Choline Agarose:

• Ligand: p-aminophenyl phosphoryl choline
• Support: crosslinked 6% beaded agarose
• Binding capacity: greater than 3 mg of human C-reactive protein per milliliter of settled resin
• Format: 50% resin slurry in 0.02% sodium azide
• Binding condition: Tris or borate-buffered saline (pH 8 to 8.5) with 1 to 2 mM calcium chloride
• Elution condition: Tris or borate-buffered saline (pH 8 to 8.5) with 2 mM EDTA
• Reusable: resin can be regenerated and reused several times

The immobilized phosphoryl choline is a beaded agarose affinity resin for purification of C-reactive protein (CRP) from plasma, ascites and other biological fluids. CRP is a pentameric (pentraxin) protein that is classified as an acute phase reactant because of its high level of production during inflammation. The protein binds to phosphoryl choline groups on the surface of microbes and has been linked to several biological functions including activation of the classical complement pathway, enhancement of phagocytosis and interaction with certain subpopulations of T-lymphocytes. In the late 1970s, Volanakis and colleagues discovered a method of isolating and studying C-reactive protein by using the protein's affinity for phosphoryl choline that has been immobilized to a porous solid chromatography support.

Pierce™ High Capacity Endotoxin Removal Spin Columns, 1 mL Thermo Scientific™

Thermo Scientific Pierce High Capacity Endotoxin Removal Resin binds to reduce endotoxin levels in protein samples by ≥99% in 1 hour; with this spin cup format, protein recovery is ≥85%.

Features of High Capacity Endotoxin Removal Resin Spin Columns:

High capacity—bind up to 2,000,000 EU/mL to eliminate >99% of endotoxins
Selective—recover ≥85% of your protein sample
High performance—meets FDA guidelines by reducing final EU concentration to <5 EU/mL
Fast—our new spin cup format enables endotoxin depletion within 1 hour
Clean—single-use spin columns avoid cross contamination of samples
Optimized—spin cups are optimized for different sample volumes

The Pierce High Capacity Endotoxin Removal Resin combines porous cellulose beads and an FDA-approved food preservative, poly(ε-lysine), as an affinity ligand to selectively bind endotoxins. The modified polylysine affinity ligand eliminates the toxicity concerns associated with alternative technologies using polymixin B ligands and sodium deoxycholate buffers.

Requires:
Endotoxin-free water
Applications:

Eliminate endotoxins from protein or antibody samples prior to cell transfection or animal injection

The presence of endotoxins in biologically derived products prepared for research, vaccination, or therapeutic use is a major concern due to the diverse and potentially harmful biological response to these molecules. Endotoxin contamination in proteins purified from bacterial cultures is due to residual lipopolysaccharides (LPS) derived from the outer cell membrane of gram-negative bacteria such as E. coli. Endotoxin-contaminated protein samples transfected into cells or injected into an animal host can initiate a strong immune response resulting in a Systemic Inflammatory Response Syndrome (SIRS) and/or sepsis. Traditional endotoxin removal resins that use polymixin B and sodium deoxycholate to clear endotoxins from a sample also have the negative side effects of neurotoxicity and acute renal tubular necrosis.

Pierce High Capacity Endotoxin Removal Resin is a porous cellulose bead that has been surface-modified with covalently attached polylysine chains that have a high affinity for LPS but lacks the toxicity of other endotoxin-binding molecules. With a 2,000,000-EU/mL binding capacity, endotoxin levels can be reduced by 99% in samples containing initial endotoxin levels of 10,000 EU/mL. Typical protein samples processed with the High Capacity Endotoxin Removal Resin have a final endotoxin concentration below 5 EU/mL. This spin cup format is a fast, single-use method to remove endotoxins from protein samples in as little as 1 hour. The spin cups use a batch format to bind and remove endotoxins while allowing for >85% protein recovery.

One endotoxin unit/mL (EU/mL) equals approximately 0.1ng endotoxin/mL of solution.

More Product Data
Removing endotoxins using a spin-column format
Eliminate endotoxins from protein and antibody samples

Related Products
Pierce™ High Capacity Endotoxin Removal Resin
Pierce™ High Capacity Endotoxin Removal Spin Columns, 0.25 mL
Pierce™ High Capacity Endotoxin Removal Spin Columns, 0.5 mL

Pierce™ Chromogenic Endotoxin Quant Kit Thermo Scientific™

The Thermo Scientific Pierce Chromogenic Endotoxin Quant Kit is a highly sensitive endpoint assay that accurately measures and detects endotoxin (lipopolysaccharide) in a protein, peptide, nucleic acid, or antibody sample using the amebocyte lysate assay. The kit enables detection within two linear sensitivity ranges of 0.01–0.1 EU/mL and 0.1–1.0 EU/mL.

Features and benefits of the Pierce Chromogenic Endotoxin Quant Kit include:
Highly sensitive with a broad range—detect as little as 0.01 EU/mL to 1 EU/mL
Specific—no interference from ß-glucans and suitable for wide range of samples, including protein, vaccine, plasmid, DNA, RNA
Fast—perform assay in as little as 20 minutes
End-point chromogenic assay—measure with a standard spectrophotometer or plate reader at 405–410 nm

The Pierce Chromogenic Endotoxin Quant Kit is an end-point chromogenic endotoxin detection assay based on the amebocyte lysate method, which measures endotoxin through the interaction of the endotoxin with the proenzyme Factor C found in circulating amebocytes of the horseshoe crab. The proteolytic activity of this proenzyme is activated in the presence of lipopolysaccharides (endotoxins) derived from the outer cell membrane of gram-negative bacteria such as E.coli. Endotoxin levels are determined by measuring the activity of Factor C in the presence of a synthetic peptide substrate that releases p-nitroaniline (pNA) after proteolysis, producing a yellow color that can be measured at an absorbance of 405 nm.

Endotoxin levels in the samples are accurately determined using the included endotoxin standard of known concentration that is derived from E.coli strain O111: B4. Determining endotoxin levels is important to assess the efficiency of endotoxin removal methods and prevent endotoxic shock, inflammation, and/or sepsis in tissue culture cells and animals injected with endotoxin-contaminated proteins.

Applications: quantitation of endotoxin levels in a protein, peptide, antibody, or nucleic acid samples

Related products:
Pierce High Capacity Endotoxin Removal Resin
Pierce High Capacity Endotoxin Removal Spin Columns

Detoxi-Gel™ Endotoxin Removing Columns, 1 mL Thermo Scientific™

Thermo Scientific Detoxi-Gel Endotoxin Removing Gel uses Polymixin B ligand immobilized on beaded affinity resin to bind and extract endotoxins from antibody or protein samples.

Detoxi-Gel Endotoxin Removing Gel and quickly remove endotoxins (lipopolysaccharides or LPS) from samples. Even low levels of endotoxins can be toxic to cells or organisms and must be removed before biological samples can be introduced.

Features of Detoxi-Gel Endotoxin Removing Gel:

• Resin uses immobilized polymixin B to remove pyrogens by binding their lipid A domains
• Reduces endotoxin levels in protein solutions, cell culture media, solutions containing pharmacological components and aqueous buffers
• 1 mL of gel removes >9995 EU from a 10,000 EU challenge of LPS (greater than 99% efficiency)
• 1 mL of gel binds 4000-6000 E.U. of the lipopolysaccharide from E. coli strain 055:BS
• Protein recovery dependent upon protein type and concentration; some empirical testing required
• Stable and reusable; resin can be regenerated by stripping off the endotoxins with a 1% deoxycholate solution in pyrogen-poor water; no loss in binding capacity even after 10 regenerations

Related Products
Detoxi-Gel™ Endotoxin Removing Gel

CaptureSelect™ Biotin Anti-FIX Conjugate Thermo Scientific™

CaptureSelect™ Biotin Anti-FIX Conjugate consists of a 13 kDa camelid antibody fragment (affinity ligand) with high affinity and selectivity for both recombinant and plasma derived human Factor IX (FIX). The affinity ligand is chemically conjugated to biotin via an appropriate spacer that retains the binding reactivity of the ligand when used in combination with streptavidin-based conjugates or streptavidin pre-coated surfaces.

The CaptureSelect™ Biotin Anti-FIX Conjugate allows you to:

Detect, quantitate, and characterize recombinant and plasma derived human FIX

Applications for CaptureSelect™ Biotin Anti-FIX Conjugate include ELISA, Western blot, Gyrolab™-based immunoassays, and label-free detection platforms such as those based on surface plasmon resonance (Biacore™ and IBIS-MX96 systems) and bio-layer interferometry (ForteBio™ Octet™ systems).

Pierce™ High Capacity Endotoxin Removal Resin Thermo Scientific™

Thermo Scientific Pierce High Capacity Endotoxin Removal Resin binds to reduce endotoxin levels in protein samples by ≥99% in 1 hour while maximizing protein recovery. Use this endotoxin removal resin slurry to pack a custom column that can be re-used up to 10 times with no loss in endotoxin removal efficiency.

Features of High Capacity Endotoxin Removal Resin:

High capacity—bind up to 2,000,000 EU/mL to eliminate >99% of endotoxins
Durable—re-use resin up to 10 times with no loss in performance
Selective—recover ≥85% of your protein sample
High performance—meets FDA guidelines by reducing final EU concentration to <5 EU/mL
Economical—large-volume discounts available

The Pierce High Capacity Endotoxin Removal Resin combines porous cellulose beads and an FDA-approved food preservative, poly(ε-lysine), as an affinity ligand to selectively bind endotoxins. Endotoxin levels in protein samples are reduced by ≥99% in as little as 1 hour using our spin cup format, and protein recovery is ≥85%. The modified polylysine affinity ligand eliminates the toxicity concerns associated with alternative technologies using polymixin B ligands and sodium deoxycholate buffers.

Requires:
Endotoxin-free water
Applications:

Eliminate endotoxins from protein or antibody samples prior to cell transfection or animal injection

The presence of endotoxins in biologically derived products prepared for research, vaccination, or therapeutic use is a major concern due to the diverse and potentially harmful biological response to these molecules. Endotoxin contamination in proteins purified from bacterial cultures is due to residual lipopolysaccharides (LPS) derived from the outer cell membrane of gram-negative bacteria such as E. coli. Endotoxin-contaminated protein samples transfected into cells or injected into an animal host can initiate a strong immune response resulting in a Systemic Inflammatory Response Syndrome (SIRS) and/or sepsis. Traditional endotoxin removal resins that use polymixin B and sodium deoxycholate to clear endotoxins from a sample also have the negative side effects of neurotoxicity and acute renal tubular necrosis.

Pierce High Capacity Endotoxin Removal Resin is a porous cellulose bead that has been surface-modified with covalently attached polylysine chains that have a high affinity for LPS but lacks the toxicity of other endotoxin-binding molecules. With a 2,000,000-EU/mL binding capacity, endotoxin levels can be reduced by 99% in samples containing initial endotoxin levels of 10,000 EU/mL. Typical protein samples processed with the High Capacity Endotoxin Removal Resin have a final endotoxin concentration below 5 EU/mL. This resin is offered in a slurry format for custom packing of endotoxin removal columns for gravity flow or flow rates ranging from 10-15 mL/hour.

One endotoxin unit/mL (EU/mL) equals approximately 0.1ng endotoxin/mL of solution.

More Product Data
Removing endotoxins using a spin-column format
Eliminate endotoxins from protein and antibody samples

Related Products
Pierce™ High Capacity Endotoxin Removal Spin Columns, 0.25 mL

Pierce™ Albumin/IgG Removal Kit Thermo Scientific™

The Thermo Scientific Pierce Albumin/IgG Removal Kit is optimized to decrease the abundant albumin and antibody components of human serum sample in preparation for 2D electrophoresis and other protein profiling methods. By eliminating most of these two proteins, which account for nearly 70% of total serum proteins, other less abundant proteins of interest can be more easily detected and studied.

Features of the Albumin/IgG Removal Kit:

Optimized for 2D sample prep—microcentrifuge spin cup method is scaled and optimized for treating 10 µL human serum samples for 2D electrophoresis
Efficient—kit removes nearly all HSA and IgG, enabling low-abundance proteins to be detected in 2D gels
Fast—kit processes samples in less than 40 minutes
Scalable—use different amounts of affinity resin to match sample volume and concentration

This dye/Protein A-based kit uses a mixture of Cibacron Blue Dye and Protein A agarose affinity resin. The dye binds to albumin with relatively good specificity, and Protein A binds to many species and subclasses of IgG. The Thermo Scientific Pierce Albumin/IgG Removal Kit is economical and can be used to treat samples from other species besides human (e.g., monkey, swine, rabbit). The kit is also scalable and sufficient to process 25 samples, each containing 600µg of total serum protein (about 10µL of serum).

Low-abundant proteins in human serum and plasma can provide information about human diseases. However, human fluid analysis is often complicated by the presence of high concentrations of albumin and IgG, which can make up more than 70% of total serum protein (Figure, Panel A). The Thermo Scientific Pierce Albumin/IgG Removal Kit uses a classical mixture of Cibacron Blue Dye and Protein A resin for economical removal of albumin and IgG. This kit can process up to 25 samples of approximately 600 µg of serum using an optimized protocol to minimize nonspecific protein interactions and sample dilution (Figure, Panel C).

Pierce™ LAL Chromogenic Endotoxin Quantitation Kit Thermo Scientific™

The Thermo Scientific Pierce LAL Chromogenic Endotoxin Quantitation Kit measures the amount of endotoxin in a protein, peptide or antibody sample using the Limulus Amebocyte Lysate (LAL) assay.

Features of the LAL Chromogenic Endotoxin Quantitation Kit:

Sensitive—detect as little as 0.1 EU/mL (approx. 0.01ng endotoxin per mL)
Fast—perform this assay in 30 minutes using a 96-well microplate
AccurateE. coli O111:B4 standard in each kit enables accurate endotoxin quantitation
Versatile—405nm absorbance reading is compatible with common ELISA plate readers

The endotoxin concentration in a sample is measured using the Pierce LAL Chromogenic Endotoxin Quantitation Kit via a chromogenic signal generated in the presence of endotoxins. Samples can be measured on a microplate absorbance reader at 405nm. A standard curve is created using the E. coli endotoxin standard included with each kit to calculate endotoxin levels as low as 0.1 EU/mL, where one endotoxin unit/mL (EU/mL) equals approximately 0.1ng endotoxin/mL of solution. Protein and antibody samples can be assayed in about 30 minutes. Determining endotoxin levels is important to assess the efficiency of endotoxin removal methods and prevent endotoxic shock, inflammation and/or sepsis in tissue culture cells and animals injected with endotoxin contaminated proteins.

Includes:
Kit contains Limulus Amebocyte Lysate (LAL), E. coli endotoxin standard, chromogenic substrate and endotoxin-free water to prepare assay reagents.

Applications:
Quantitation of endotoxin levels in a protein, peptide or antibody solution

The LAL method for measuring endotoxin is based on the interaction of endotoxins with the proenzyme Factor C found in circulating amebocytes of the horseshoe crab Limulus polyphemus. The proteolytic activity of this proenzyme is activated in the presence of lipopolysaccharides (endotoxins) derived from the outer cell membrane of gram-negative bacteria such as E. coli. The Chromogenic Limulus Amebocyte Lysate assay measures endotoxin levels by measuring the activity of this protease in the presence of a synthetic peptide substrate that releases p-nitroaniline (pNA) after proteolysis, producing a yellow color that can be measured by reading the absorbance at 405nm.

To accurately measure endotoxin levels in a sample, the LAL assay uses an endotoxin standard of known concentration that is derived from E. coli strain O111:B4. This standard is provided with each kit and is used to create a standard curve. The endotoxin concentration is determined by extrapolating the absorbance of an unknown sample against this standard curve, similar to ELISA or total protein quantitation assays.

More Product Data
Removing endotoxins using a spin-column format
Eliminate endotoxins from protein and antibody samples

Detoxi-Gel™ Endotoxin Removing Gel Thermo Scientific™

Thermo Scientific Detoxi-Gel Endotoxin Removing Gel uses Polymixin B ligand immobilized on beaded affinity resin to bind and extract endotoxins from antibody or protein samples.

Detoxi-Gel Endotoxin Removing Gel and quickly remove endotoxins (lipopolysaccharides or LPS) from samples. Even low levels of endotoxins can be toxic to cells or organisms and must be removed before biological samples can be introduced.

Features of Detoxi-Gel Endotoxin Removing Gel:

• Resin uses immobilized polymixin B to remove pyrogens by binding their lipid A domains
• Reduces endotoxin levels in protein solutions, cell culture media, solutions containing pharmacological components and aqueous buffers
• 1 mL of gel removes >9995 EU from a 10,000 EU challenge of LPS (greater than 99% efficiency)
• 1 mL of gel binds 4000-6000 E.U. of the lipopolysaccharide from E. coli strain 055:BS
• Protein recovery dependent upon protein type and concentration; some empirical testing required
• Stable and reusable; resin can be regenerated by stripping off the endotoxins with a 1% deoxycholate solution in pyrogen-poor water; no loss in binding capacity even after 10 regenerations

Related Products
Detoxi-Gel™ Endotoxin Removing Columns, 1 mL

Pierce™ Boronic Acid Resin Thermo Scientific™

Thermo Scientific Pierce Boronic Acid Resin enables ribonucleotide and oligonucleotide RNA isolation.

Features of Boronic Acid Resin:

• Ligand: boronic acid
• Support: beaded polyacrylamide (1800 MW exclusion limit)
• Spacer: m-aminophenyl group
• Loading: 100 µMol boronate per milliliter of settled resin
• Capacity: at least 99% binding and recovery of 110 µMol AMP per milliliter of settled resin
• Format: 50% resin slurry in 0.05% sodium azide

The immobilized boronic acid is an easy-to-use polyacrylamide affinity support for purification of ribonucleotides and other small molecular weight compounds that contain cis-diol groups. The ligand (m-aminophenyl-boronic acid) binds to the cis-diol groups on the sugar portion of nucleotides, forming a reversible five-member ring complex. After washing away non-bound molecules from the sample, the complex can be dissociated and the nucleotide eluted by lowering the pH or by addition of sorbitol.

The polyacrylamide support used in this product excludes molecules greater than 1800 MW from entering the internal spaces of the beads, allowing only smaller molecules to interact and bind with the full measure of boronate ligand. Consequently, the resin may not purify glycosylated proteins efficiently, although it has been used successfully for this application. Boronic acid affinity chromatography has been used to isolate ribonucleoside, to purify nucleosidyl peptide, separate ribonucleosidases in tissue extracts, separate RNA and oligoribonucleotide, and determine of the amount of non-enzymatic glycosylation present in peripheral nerve from diabetic and control rats and dogs.

Pierce™ GST Agarose Thermo Scientific™

Thermo Scientific Immobilized GST is glutathione S-transferase from Schistosoma japonicum that has been covalently immobilized on crosslinked, 6% beaded agarose.

Features of GST Agarose:

• Provides purification of specific antibodies from those directed against the GST tag
• Reusable—bound antibodies can be eluted with 6 M guanidinium chloride allowing for 10 purifications with the support

Antibody molecules recognizing the GST portion of a recombinant fusion protein can be removed from the desired antibody by passing antiserum over an immobilized GST column. GST antibodies will bind to the immobilized GST column, whereas antibodies against other fusion proteins will pass through the column unrestrained. The expression of foreign proteins in E. coli using a glutathione S-transferase (GST) fusion system is a method of choice for studying the function of particular proteins, for purifying interacting factors and for generating antibodies against fused proteins.

Related Products
Pierce™ GST Agarose Columns, 2 mL
Pierce™ GST (glutathione-S-transferase)
Results per page
    spinner