Shop All Protease Inhibitors and Phosphatase Inhibitors

Halt™ Phosphatase Inhibitor Cocktail Thermo Scientific™

Thermo Scientific Halt Phosphatase Inhibitor Single Use Cocktail preserves the phosphorylation state of proteins during and after cell lysis or tissue protein extraction.

Features of Halt Phosphatase Inhibitor Single Use Cocktail:

Devoid of protease inhibitors—To protect against both proteases and phosphatases, use Halt Protease and Phosphatase Inhibitor Cocktail.
Dialyzable— Either dialyze or desalt sample to effectively remove inhibitors from cell extracts before performing procedures that are discovered to be incompatible with cocktail components.
Supplied as a 100X concentrate—effective when used at a final concentration of 1X; however, samples that contain particularly high levels of phosphatase activity may require a final concentration of 2-3X.
Compatibility— compatible with most detergent-based cell lysis reagents, including all Thermo Scientific Pierce Cell Lysis Solutions.
All-in-one format—inhibits both serine-threonine phosphatases and protein tyrosine phosphatases
Compatiblewith popular protein assays—works with standard protein assays, including BCA and coomassie (Bradford) assays
Convenient storage—refrigerator (2 to 8°C) storage saves valuable space in the freezer and provides for immediate use

Halt Phosphatase Inhibitor Cocktail protects phosphoproteins from serine/threonine phosphatases as well as protein tyrosine phosphatases (PTPs) during cell lysis. The cocktail contains a mixture of four inhibitors of broad specificity, including sodium fluoride, sodium orthovanadate, sodium pyrophosphate and beta-glycerophosphate. Conveniently packaged in a 100X format, the cocktail is stable at 4°C and is suitable for use with any cell or tissue extract. The cocktail preserves protein phosphorylation in immunoprecipitation and kinase assays.

Phosphorylation and dephosphorylation comprise a molecular on/off switch that regulates many key biological pathways within the cell, including signal transduction, cell division and apoptosis. To effectively study phosphorylation activation states, one must inactivate phosphatases to prevent their uncontrolled activity upon cell lysis. Halt Phosphatase Inhibitor Cocktail accomplishes this task.

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Pierce™ Phosphatase Inhibitor Mini Tablets

Halt™ Protease Inhibitor Cocktail, EDTA-free (100X) Thermo Scientific™

Thermo Scientific Halt Protease Inhibitor Cocktail, EDTA-free (100X) are ready-to-use concentrated stock solutions of protease inhibitors for addition to samples to prevent proteolytic degradation during cell lysis and protein extraction.

Features of Halt Protease Inhibitor Cocktail, EDTA-free (100X):

Convenient—the refrigerator-stable, 100X liquid format is more effective and easier to use than individual inhibitors or other formats; just pipette exactly the amount you need
No proprietary ingredients—fully disclosed formulation contains optimized concentrations of six broad-spectrum protease inhibitors AEBSF, aprotinin, bestatin, E-64, leupeptin and pepstatin A stabilized in high-quality dimethylsulfoxide (DMSO)
Multiple package sizes—choose among several package sizes, including single-use microtubes that keep protease inhibitors fresh and reduce the risk of reagent contamination
EDTA-free formulations—ensures compatibility with isoelectric focusing
Lysis buffer compatible—use with Thermo Scientific Pierce Cell Lysis Buffers or nearly any other commercial or homemade detergent-based lysis reagent. Add 10 µL of concentrated cocktail per 1 mL of lysis buffer to ensure complete protection of the resulting protein extract.

Applications:
• Protection of intact, active cellular proteins from degradation by endogenous proteases
• Inhibition of protease activity from lysates produced by a variety of methods including Thermo Scientific Pierce Cell Lysis Reagents, other commercially formulated cell or tissue lysis products, sonication, French press, blender homogenization or repetitive freeze/thaw cycling
• Screening of extracts for proteolytic activity
• The study of proteolysis in the regulation of cellular processes

Proteases make up a large category of enzymes, including endopeptidases and exopeptidases, which catalyze the hydrolytic breakdown of proteins into peptides or amino acids in a wide variety of cell and tissue types. Proteases are regulated and compartmentalized in living cells, but cell lysis releases and mixes them with other extracted proteins. Their activity can thus lead to the loss of a large number of valuable proteins, adversely affecting downstream applications. For this reason, it is common practice to spike cell lysis buffers or cell extracts with a cocktails of compounds known to inactivate or inhibit proteases. Halt Protease Inhibitor Cocktails effectively inhibit serine-proteases, cysteine-proteases, aspartic acid-proteases and aminopeptidases that are typically present in cellular lysate samples. Regular and EDTA-free formulations provide for inhibition of metalloproteases and compatibility with 2D electrophoresis, respectively.

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Halt™ Protease Inhibitor Single-Use Cocktail (100X)

Halt™ Protease and Phosphatase Inhibitor Cocktail (100X) Thermo Scientific™

Thermo Scientific Halt Protease and Phosphatase Inhibitor Cocktail (100X) provides the convenience of a single solution with full protein sample protection for cell and tissue lysates.

Features of Halt Protease and Phosphatase Inhibitor Cocktail (100X):

Complete protection—all-in-one cocktail contains both protease and phosphatase inhibitors
Multiple package sizes—choose from four convenient packages sizes to match nearly any scale
Convenient —the 100X liquid formulation is much easier and more effective to use than individual inhibitors and other formats
Easy storage—aqueous-based format allows for convenient cold-room storage without freezing, by contrast with DMSO-based cocktails
Compatible—can be used in mass spectrometry (MS) because it does not contain AEBSF, which can cause peaks to shift
Validated—fully compatible with Thermo Scientific Pierce Cell Lysis Buffers

Halt Protease and Phosphatase Inhibitor Cocktail is specifically optimized to protect proteins from degradation during extraction and purification. The cocktail contains inhibitors against the major classes of proteases and phosphatases, targeting aminopeptidases, cysteine and serine proteases and serine/threonine and protein tyrosine phosphatases. To promote the inhibition of metalloproteases, 0.5M EDTA is provided in a separate tube.

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Halt™ Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X)

Halt™ Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X) Thermo Scientific™

Thermo Scientific Halt Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X) provides the convenience of a single solution with full protein sample protection for cell and tissue lysates.

Features of Halt Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X):

Complete protection—all-in-one cocktail contains both protease and phosphatase inhibitors
Multiple package sizes—choose from four convenient packages sizes to match nearly any scale
Convenient —the 100X liquid formulation is much easier and more effective to use than individual inhibitors and other formats
Easy storage—aqueous-based format allows for convenient cold-room storage without freezing, by contrast with DMSO-based cocktails
Compatible—can be used in mass spectrometry (MS) because it does not contain AEBSF, which can cause peaks to shift
Validated—fully compatible with Thermo Scientific Pierce Cell Lysis Buffers

Halt Protease and Phosphatase Inhibitor Cocktail is specifically optimized to protect proteins from degradation during extraction and purification. The cocktail contains inhibitors against the major classes of proteases and phosphatases, targeting aminopeptidases, cysteine and serine proteases and serine/threonine and protein tyrosine phosphatases.

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Halt™ Protease and Phosphatase Inhibitor Cocktail (100X)

Pierce Protease Inhibitor Tablets Thermo Scientific™

Thermo Scientific Pierce Protease Inhibitor Tablets contain broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation during cell lysis and protein extraction experiments.

Features of Protease Inhibitor Tablets:
Improved formulation—tablets dissolve quickly to form a clear solution
Economical—more cost effective than other commercially available formulations for equivalent volumes
Convenient—ready-to-use, fully disclosed, broad-spectrum formulations
Compatible—use directly with Pierce BCA assays

Pierce Protease Inhibitor Tablets protect proteins from proteolytic degradation through inhibition of the serine proteases, cysteine proteases, aspartic acid proteases, and aminopeptidases that are typically present in cell lysate samples. The tablets contain AEBSF, aprotinin, bestatin, E-64, leupeptin, and pepstatin A. Stable for storage at 4°C for up to 12 months, each tablet is sufficient for 50 mL of solution, and is suitable for reagent preparation prior to protein extraction from tissue and cultured cells.

Proteases make up a large category of enzymes, including endopeptidases and exopeptidases, which catalyze the hydrolytic breakdown of proteins into peptides or amino acids. Proteases reside in cellular compartments, but upon cell lysis the proteases are released, resulting in the degradation of valuable proteins. For this reason, it is common practice to add multiple compounds known to inactivate or inhibit proteases to cell lysis buffers or cell extracts.

To use Pierce Protease Inhibitor Tablets, simply dissolve 1 tablet in 50 mL of buffer or lysate. The protease inhibitor formulation is compatible with most detergent-based cell lysis reagents, including Pierce Cell Lysis solutions. Pierce Protease Inhibitor Tablets do not contain phosphatase inhibitors. If necessary, phosphatase inhibitors, such as Pierce Phosphatase Inhibitor Mini Tablets, can be added along with the protease inhibitor tablets.

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Pierce Protease Inhibitor Tablets, EDTA-free
Pierce Protease Inhibitor Mini Tablets
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Pierce Protease and Phosphatase Inhibitor Mini Tablets

Halt™ Protease Inhibitor Cocktail (100X) Thermo Scientific™

Thermo Scientific Halt Protease Inhibitor Cocktail (100X) are ready-to-use concentrated stock solutions of protease inhibitors for addition to samples to prevent proteolytic degradation during cell lysis and protein extraction.

Features of Halt Protease Inhibitor Cocktail, (100X):

Convenient—the refrigerator-stable, 100X liquid format is more effective and easier to use than individual inhibitors or other formats; just pipette exactly the amount you need
No proprietary ingredients—fully disclosed formulation contains optimized concentrations of six broad-spectrum protease inhibitors AEBSF, aprotinin, bestatin, E-64, leupeptin and pepstatin A stabilized in high-quality dimethylsulfoxide (DMSO)
Multiple package sizes—choose among several package sizes, including single-use microtubes that keep protease inhibitors fresh and reduce the risk of reagent contamination
Regular formulation—includes a separate vial of EDTA solution for optional metalloprotease inhibition
Lysis buffer compatible—use with Thermo Scientific Pierce Cell Lysis Buffers or nearly any other commercial or homemade detergent-based lysis reagent. Add 10 µL of concentrated cocktail per 1 mL of lysis buffer to ensure complete protection of the resulting protein extract.

Applications:
• Protection of intact, active cellular proteins from degradation by endogenous proteases
• Inhibition of protease activity from lysates produced by a variety of methods including Thermo Scientific Pierce Cell Lysis Reagents, other commercially formulated cell or tissue lysis products, sonication, French press, blender homogenization or repetitive freeze/thaw cycling
• Screening of extracts for proteolytic activity
• The study of proteolysis in the regulation of cellular processes

Proteases make up a large category of enzymes, including endopeptidases and exopeptidases, which catalyze the hydrolytic breakdown of proteins into peptides or amino acids in a wide variety of cell and tissue types. Proteases are regulated and compartmentalized in living cells, but cell lysis releases and mixes them with other extracted proteins. Their activity can thus lead to the loss of a large number of valuable proteins, adversely affecting downstream applications. For this reason, it is common practice to spike cell lysis buffers or cell extracts with a cocktails of compounds known to inactivate or inhibit proteases. Halt Protease Inhibitor Cocktails effectively inhibit serine-proteases, cysteine-proteases, aspartic acid-proteases and aminopeptidases that are typically present in cellular lysate samples. Regular and EDTA-free formulations provide for inhibition of metalloproteases and compatibility with 2D electrophoresis, respectively.

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Halt™ Protease Inhibitor Single-Use Cocktail, EDTA-free (100X)

PMSF Protease Inhibitor Thermo Scientific™

Thermo Scientific PMSF is a protease inhibitor that reacts with serine residues to inhibit trypsin, chymotrypsin, thrombin and papain.

Features of PMSF:

• Targets serine proteases, including trypsin, chymotrypsin, thrombin and papain
• Effective in most protein solutions at 0.1 to 1 mM final concentration
• Because PMSF has limited water solubility, it must be dissolved in a small amount of solvent, such as ethanol, methanol or isopropyl alcohol before addition to a buffer

PMSF is an abbreviation for phenylmethylsulfonyl fluoride, the most common chemical name for this small compound. In addition to inhibiting serine proteases, PMSF will also inhibit cysteine proteases, like papain (reversible by DTT treatment), and mammalian acetylcholinesterase.

Properties of PMSF:
• Synonyms: alpha-Toluenesulfonyl fluoride; Benzylsulfonyl fluoride; Phenylmethylsulfonyl fluoride
• Formula: C6H5CH2SO2F
• Molecular weight: 174.19
• CAS Number: 329-98-6; EC Number: 206-350-2
• R/S Codes: R 25-34; S 26-36/37/39-45
• Storage conditions: room temperature in a dry place protected from light
• Working solution: dissolve PMSF in isopropanol at 1.74 mg/mL (10 mM); store in aliquots at -20°C.

Specifications of PMSF (Part No. 36978):
• Appearance: white powder
• Purity: > 99.0 %
• Melting point: 90-95°C
• Humidity: < 0.1 %

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Pierce™ Protease and Phosphatase Inhibitor Mini Tablets, EDTA Free
Pierce™ Protease Inhibitor Mini Tablets, EDTA Free

Pierce™ Protease Inhibitor Mini Tablets, EDTA-free Thermo Scientific™

Thermo Scientific Pierce Protease Inhibitor Mini Tablets, EDTA-free, contain broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation during cell lysis and protein extraction experiments.

Features of EDTA-free Protease Inhibitor Mini Tablets:
Improved formulation—tablets dissolve quickly to form a clear solution
Economical—more cost effective than other commercially available formulations for equivalent volumes
Convenient—ready-to-use, fully disclosed, broad-spectrum formulations
Compatible—use directly with Pierce BCA assays
EDTA-free—formulated without EDTA, a metalloproteinase inhibitor (only for EDTA-free formulations)

Pierce Protease Inhibitor Mini Tablets protect proteins from proteolytic degradation through inhibition of the serine proteases, cysteine proteases, aspartic acid proteases, and aminopeptidases that are typically present in cell lysate samples. The tablets contain AEBSF, aprotinin, bestatin, E-64, leupeptin, and pepstatin A. Stable for storage at 4°C for up to 12 months, each mini tablet is sufficient for 10 mL of solution, and is suitable for reagent preparation prior to protein extraction from tissue and cultured cells.

Proteases make up a large category of enzymes, including endopeptidases and exopeptidases, which catalyze the hydrolytic breakdown of proteins into peptides or amino acids. Proteases reside in cellular compartments, but upon cell lysis the proteases are released, resulting in the degradation of valuable proteins. For this reason, it is common practice to add multiple compounds known to inactivate or inhibit proteases to cell lysis buffers or cell extracts.

To use Pierce Protease Inhibitor Mini Tablets, simply dissolve 1 tablet in 10 mL of buffer or lysate. The protease inhibitor formulation is compatible with most detergent-based cell lysis reagents, including Pierce Cell Lysis solutions. Pierce Protease Inhibitor Mini Tablets do not contain phosphatase inhibitors. If necessary, phosphatase inhibitors, such as Pierce Phosphatase Inhibitor Mini Tablets, can be added along with the protease inhibitor tablets.

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Pierce Protease Inhibitor Mini Tablets
Pierce Protease Inhibitor Tablets
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Pierce Protease and Phosphatase Inhibitor Mini Tablets

Pierce™ Protease Inhibitor Tablets, EDTA-free Thermo Scientific™

Thermo Scientific Pierce Protease Inhibitor Tablets, EDTA-free, contain broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation during cell lysis and protein extraction experiments.

Features of EDTA-free Protease Inhibitor Tablets:
Improved formulation—tablets dissolve quickly to form a clear solution
Economical—more cost effective than other commercially available formulations for equivalent volumes
Convenient—ready-to-use, fully disclosed, broad-spectrum formulations
Compatible—use directly with Pierce BCA assays
EDTA-free—formulated without EDTA, a metalloproteinase inhibitor (only for EDTA-free formulations)

Pierce Protease Inhibitor Tablets protect proteins from proteolytic degradation through inhibition of the serine proteases, cysteine proteases, aspartic acid proteases, and aminopeptidases that are typically present in cell lysate samples. The tablets contain AEBSF, aprotinin, bestatin, E-64, leupeptin, and pepstatin A. Stable for storage at 4°C for up to 12 months, each tablet is sufficient for 50 mL of solution, and is suitable for reagent preparation prior to protein extraction from tissue and cultured cells.

Proteases make up a large category of enzymes, including endopeptidases and exopeptidases, which catalyze the hydrolytic breakdown of proteins into peptides or amino acids. Proteases reside in cellular compartments, but upon cell lysis the proteases are released, resulting in the degradation of valuable proteins. For this reason, it is common practice to add multiple compounds known to inactivate or inhibit proteases to cell lysis buffers or cell extracts.

To use Pierce Protease Inhibitor Tablets, simply dissolve 1 tablet in 50 mL of buffer or lysate. The protease inhibitor formulation is compatible with most detergent-based cell lysis reagents, including Pierce Cell Lysis solutions. Pierce Protease Inhibitor Tablets do not contain phosphatase inhibitors. If necessary, phosphatase inhibitors, such as Pierce Phosphatase Inhibitor Mini Tablets, can be added along with the protease inhibitor tablets.

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Pierce Protease Inhibitor Tablets
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Pierce Protease and Phosphatase Inhibitor Mini Tablets

Aprotinin Protease Inhibitor Thermo Scientific™

Thermo Scientific Aprotinin Protease Inhibitor reversably inhibits serine proteases. Thermo Scientific Protease Inhibitors are small packages of individual protease inhibitor peptides and compounds for customized formulation or modification of protease inhibitor cocktails.

Features of Thermo Scientific Aprotinin Protease Inhibitor:

• High-quality reagents in convenient and affordable package sizes
• Purchase individually to prepare customized protease inhibitor cocktails
• Supplement Halt Protease Inhibitor Cocktails, whose formulations are fully disclosed, to achieve desired concentrations of specific component reagents

The individual reagents include AEBSF, aprotinin, bestatin, E64, leupeptin, pepstatin A and PMSF. Although Thermo Scientific Halt Protease Inhibitor Cocktails provide convenient and optimized broad-spectrum protease inhibition for routine cell lysis and assay needs, certain applications require a more customized approach. By offering the individual high-quality reagents used to make our cocktail products, we provide you with the tools needed to apply a protease inhibitor individually, supplement a cocktail or combine several components to make customized protease inhibitor cocktails for specialized applications.

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Pierce™ Protease and Phosphatase Inhibitor Mini Tablets, EDTA Free
Pierce™ Protease Inhibitor Mini Tablets, EDTA Free

Pierce™ Protease Inhibitor XL Capsules, EDTA-free Thermo Scientific™

Thermo Scientific Pierce Protease Inhibitor XL Capsules, EDTA-free, contain a broad spectrum formulation of four protease inhibitors and each capsule is sufficient for 500 mL of solution.

Features of EDTA-free Protease Inhibitor XL Capsules:
Broad spectrum formulation—contains AEBSF, bestatin, E-64, and pepstatin A for better protection versus PMSF
Large volume format—one capsule is sufficient for 500 mL of solution
Convenient—ready-to-use, dissolves clearly into solution within three minutes
Compatible—use directly with Pierce protein assays
EDTA-free—formulated without EDTA, a metalloproteinase inhibitor

Pierce Protease Inhibitor XL Capsules protect proteins from proteolytic degradation through inhibition of the serine proteases, cysteine proteases, aspartic acid proteases, and aminopeptidases that are typically present in cell lysate samples. The tablets contain AEBSF, bestatin, E-64, and pepstatin A. Each capsule is sufficient for 500 mL of solution, and is suitable for reagent preparation prior to protein extraction from tissue and cultured cells.

Proteases make up a large category of enzymes, including endopeptidases and exopeptidases, which catalyze the hydrolytic breakdown of proteins into peptides or amino acids. Proteases reside in cellular compartments, but upon cell lysis the proteases are released, resulting in the degradation of valuable proteins. For this reason, it is common practice to add multiple compounds known to inactivate or inhibit proteases to cell lysis buffers or cell extracts. PMSF is commonly used for protease protection in large volumes, but does not offer the same level of protection as the capsule formulation.

To use Pierce Protease Inhibitor XL Capsules, simply twist the capsule to open and empty the contents of one capsule into 500 mL of solution. The protease inhibitor formulation is compatible with most detergent-based cell lysis reagents, including Pierce cell lysis solutions. Pierce Protease Inhibitor XL Capsules do not contain phosphatase inhibitors. If necessary, phosphatase inhibitors, such as Pierce phosphatase inhibitors, can be added along with the protease inhibitor capsules.

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T-PER Tissue Protein Extraction Reagent

Pierce™ Phosphatase Inhibitor Mini Tablets Thermo Scientific™

Thermo Scientific Pierce Phosphatase Inhibitor Mini Tablets provide complete cell lysate and biological sample protection from dephosphorylation by a broad spectrum of serine and threonine phosphatases.

Features of Pierce Phosphatase Inhibitor Mini Tablets:
Economical—more cost effective than other commercially available formulations
Improved formulation—tablets dissolve quickly to form a clear solution
Complete—the tablets inhibit acid, alkaline, and protein phosphatases
Compatible—use with homebrew or commercially available cell lysis reagents and Thermo Scientific protein assays

Pierce Phosphatase Inhibitor Mini Tablets protect phosphoproteins from serine/threonine phosphatases and tyrosine phosphatases. Each Phosphatase Inhibitor Tablet contains sodium fluoride, sodium orthovanadate, sodium pyrophosphate, and beta-glycerophosphate for broad-spectrum inhibition of phosphatase activity. Tablets are stable at 4°C for up to 12 months, and each tablet is sufficient for 10 mL of solution. The Pierce Phosphatase Inhibitor Tablets are suitable for use with any cell or tissue extract to preserve protein phosphorylation for downstream applications.

Protein phosphorylation and dephosphorylation states often function as molecular on/off switches for key regulators in biological pathways within the cell, including signal transduction, cell division, and apoptosis. To effectively study phosphorylation signaling events, endogenous phosphatases must be inactivated to prevent their uncontrolled activity upon cell lysis. Pierce Phosphatase Inhibitor Tablets preserve phosphoprotein modifications by significantly reducing endogenous acid, alkaline, and protein phosphatase activity.

Pierce Phosphatase Inhibitor Mini Tablets are compatible with most detergent-based cell lysis reagents, including Pierce Cell Lysis solutions. Where incompatibilities arise, the inhibitor components can be removed by dialysis or desalting before performing these procedures.

To use Pierce Phosphatase Inhibitor Mini Tablets, simply dissolve 1 tablet in 10 mL of buffer or lysate. Pierce Phosphatase Inhibitor Mini Tablets do not contain protease inhibitors. If necessary, protease inhibitors, such as Pierce Protease Inhibitor Tablets, can be added along with the phosphatase inhibitor tablet.

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Bestatin Protease Inhibitor Thermo Scientific™

Thermo Scientific Bestatin Protease Inhibitor reversably inhibits amino peptidases. Thermo Scientific Protease Inhibitors are small packages of individual protease inhibitor peptides and compounds for customized formulation or modification of protease inhibitor cocktails.

Features of Thermo Scientific Bestatin Protease Inhibitor:

• High-quality reagents in convenient and affordable package sizes
• Purchase individually to prepare customized protease inhibitor cocktails
• Supplement Halt Protease Inhibitor Cocktails, whose formulations are fully disclosed, to achieve desired concentrations of specific component reagents

The individual reagents include AEBSF, aprotinin, bestatin, E64, leupeptin, pepstatin A and PMSF. Although Thermo Scientific Halt Protease Inhibitor Cocktails provide convenient and optimized broad-spectrum protease inhibition for routine cell lysis and assay needs, certain applications require a more customized approach. By offering the individual high-quality reagents used to make our cocktail products, we provide you with the tools needed to apply a protease inhibitor individually, supplement a cocktail or combine several components to make customized protease inhibitor cocktails for specialized applications.

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Pierce™ Protease and Phosphatase Inhibitor Mini Tablets, EDTA Free
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AcTEV™ Protease Invitrogen™

AcTEV™ Protease specifically recognizes a seven amino acid sequence (Glu-Asn-Leu-Tyr-Phe-Gln-Gly, cleaving between Gln and Gly), making it useful for removing affinity tags from fusion proteins. AcTEV™ Protease is an improved version of Tobacco Etch Virus (TEV) protease that is highly site-specific, highly active, and significantly more stable than native TEV protease, resulting in enhanced long-term activity. AcTEV™ Protease features:

• Highly specific cleavage activity
• Enhanced enzyme stability for prolonged protease activity (see figure)
• Activity over a broad temperature (+4°C to 30°C) and pH (6.0 to 8.5) range
• Six-histidine sequence to facilitate its removal from the digested protein sample
• Greater than 85% single-band purity with no nonspecific protease contaminationApplications
Incubation with AcTEV™ Protease releases the protein of interest from the fusion tag. This is an effective way to remove solubility, secretion, detection, and purification tags from recombinant proteins.Enzyme specifications
Purified from E. coli expressing the AcTEV™ Protease gene.

Unit definition
One unit of AcTEV™ Protease cleaves 85% of a 3 µg control substrate in 1 hr at 30°C.

Unit reaction conditions
50 mM Tris-HCl (pH 8.0), 0.5 mM EDTA, 1 mM DTT, 3 µg control substrate, and 1 unit enzyme in 30 µL for 1 hr at 30°C. AcTEV™ Protease is functionally tested for the absence of any nonspecific protease activity.

Pierce Protease and Phosphatase Inhibitor Mini Tablets,EDTA-free Thermo Scientific™

Thermo Scientific Pierce Protease and Phosphatase Inhibitor Mini Tablets, EDTA-free, contain both protease inhibitors and phosphatase inhibitors to provide broad-spectrum complete protection from dephosphorylation and proteolytic degradation.

Features of Protease and Phosphatase Inhibitor Mini Tablets, EDTA-free:
Improved formulation—tablets dissolve quickly to form a clear solution
Economical—more cost effective than other commercially available formulations for equivalent volumes
Convenient—ready-to-use, fully disclosed, broad-spectrum formulations
Compatible—use directly with Pierce BCA assays
EDTA-free—formulated without EDTA, a metalloproteinase inhibitor (only for EDTA-free formulations)

The Pierce Protease and Phosphatase Inhibitor Mini Tablets contain aprotinin, bestatin, E-64, and leupeptin to provide protection from degradation by serine proteases, cysteine proteases, and aspartic acid proteases. To prevent the dephosphorylation activity of serine/threonine and tyrosine phosphatases, each tablet also contains sodium fluoride, sodium orthovanadate, sodium pyrophosphate, and beta-glycerophosphate. Pierce Protease and Phosphatase Inhibitor Tablets are stable at 4°C for up to 12 months, and each tablet is sufficient for 10 mL of solution.

Pierce Protease and Phosphatase Inhibitor Mini Tablets are the first complete inhibitor tablet. Adding one tablet to 10 mL of lysis buffer provides complete protection from protease and phosphatase activity during protein extraction and protein purification. There are two formulations of this inhibitor available, one containing EDTA and one without. While both formulations have metalloprotease inhibitors, an EDTA-free formulation is available for applications that require divalent cations for protein activity. Similarly, EDTA and certain phosphatase inhibitors interfere with immobilized metal chelate affinity chromatography (IMAC) and 2D electrophoresis. Dialyze or desalt the sample before performing these procedures. The Protease and Phosphatase Inhibitor formulation is mass spectrometry-compatible because it does not contain AEBSF.

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