Shop All Protein Purification Reagents and Kits

Quant-iT™ Protein Assay Kit Invitrogen™

The Quant-iT Protein Assay Kit makes protein quantitation easy and accurate. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted BSA standards. Simply dilute the reagent, load it into the wells of a microplate, add 1-20 µL of sample, mix, then measure the fluorescence. The assay is highly selective for protein and exhibits very little protein-to-protein variation. The assay is performed at room temperature, and the signal is stable for 3 hours. Common contaminants, such as salts, solvents, or DNA—but not detergents—are well tolerated in the assay. Quant-iT DNA Assay Kits (Q33120, Q33130) and a Quant-iT RNA Assay Kit (Q33140) are also available.

CaptureSelect™ AAVX Ligand Leakage ELISA Thermo Scientific™

The CaptureSelect AAVX Ligand Leakage ELISA detects affinity ligand contamination of antibodies or antibody fragments purified using POROS CaptureSelect AAVX Affinity Matrix. The ligand, a 13-kD recombinant protein, is covalently coupled for high chemical stability and low leakage. Even when covalently attached, small amounts of the affinity ligand can leach off of the chromatography support and co-elute with the protein. This sensitive ELISA detects affinity ligand contamination of less than 1 ng/mL. The assay is designed to minimize interference and to provide accurate quantitation. POROS CaptureSelect AAVX Ligand Leakage ELISA can be used as a tool to aid in optimal purification process development and in routine quality control of in-process streams as well as final product.

View other viral vector and vaccine affinity matrix formats ›

CaptureSelect™ IgE Affinity Matrix Thermo Scientific™

Thermo Scientific CaptureSelect IgE Affinity Matrix has been designed specifically for the purification of human IgE (immunoglobuline E) from recombinant and plasma sources.

Features of this affinity matrix include:
• Purification of human IgE, binding different recombinant IgE forms
• Efficient removal of host cell proteins (HCP) and IgE aggregates
• Excellent scalability
• Non–animal-derived

Free of animal components
CaptureSelect products contain affinity ligands created by a proprietary technology based on camelid-derived single-domain antibody fragments. The ligand is a 13 kDa fragment comprised of three complementarity determining regions (CDRs) that form the antigen binding domain, efficiently produced by the yeast Saccharomyces cerevisiae in a production process free of animal components.

Main characteristics

Matrix: agarose bead
Average particle size: 65 µm
Ligand: CaptureSelect IgE affinity ligand
Ligand coupling method: aldehyde coupling
Binding capacity: >5 mg/mL resin
Elution conditions: 50 mM sodium citrate, 150 mM NaCl, pH 3.5
Formulation buffer: 20% (v/v) ethanol

Pierce™ Glutathione Chromatography Cartridges, 1 mL Thermo Scientific™

Thermo Scientific Pierce Glutathione Chromatography Cartridges are convenient, ready-to-use pre-packed devices for thepurification of GST-fusion proteins from cellular lysates.

Features of Glutathione Agarose:

High capacity—binds at least 40 mg of purified recombinant GST protein per milliliter of resin
High yield and purity—consistently purifies at least 10 mg of GST-tagged protein per milliliter of resin with greater than 90% purity
Cost-effective—resin is economically priced and can be reused at least five times without reduction in binding capacity and purification performance
Versatile—works well to purify GST-fusion proteins from bacterial lysates or use with pre-purified GST-tagged proteins to pull down protein interactions
Compatible—validated and effective for use with Thermo Scientific Cell Lysis Reagents to extract and purify from bacterial or mammalian cell cultures

The glutathione is immobilized through its central sulfhydryl onto 6% crosslinked agarose resin. Purification of GST-fusion proteins using glutathione (GSH) agarose beads is well documented and provides a one-step, high purity affinity purification. Whether the purpose is to purify large amounts of recombinant protein from over-expressing E. coli lysates or to investigate protein interactions involving GST-tagged bait proteins, Pierce Glutathione Agarose is suitable for the task.

Thermo Scientific Pierce Glutathione Agarose is also offered in three volumes of resin slurry, complete GST purification kits, two sizes of FPLC-ready chromatography cartridges, and 96-well filter plates for high through-put needs.

Pierce Glutathione Agarose effectively purifies high levels of overexpressed GST-tagged fusion proteins from bacterial lysates, such as those that are obtained with Thermo Scientific B-PER Bacterial Protein Extraction Reagents.

Pierce Glutathione Agarose performs well in batch-binding and spin-column procedures at a variety of scales. Performance equals or exceeds popular GSH resins from other suppliers.

Pierce Glutathione Agarose is a high-quality, stable and resilient affinity support. Tests confirm that no decrease in performance occurs after at least five repeated uses. These data indicated that the resin is highly resistant to structural degradation or ligand leaching during normal use.

The expression and purification of recombinant proteins is central to protein regulation, structure and function studies. The majority of recombinant proteins are expressed as fusions with short affinity tags or small proteins, such as glutathione S-transferase (GST). This protein binds specifically to reduced glutathione (GSH) in near-neutral, nondenaturing conditions (e.g., Tris buffer). Bound protein is easily dissociated (eluted) by competitive displacement with buffer containing free, reduced GSH (oxidized glutathione, GSSH is not effective for this purpose). When proteins of interest are expressed as fusions with GST and glutathione is immobilized to an solid support, this protein-substrate system enables affinity purification of recombinant proteins, as well as various other experiments with those proteins.

Related Products
Pierce™ Glutathione Chromatography Cartridges, 1 mL
Pierce™ Glutathione Spin Columns, 3 mL
Pierce™ Glutathione Agarose

CaptureSelect™ FcXP Ligand Leakage ELISA Thermo Scientific™

The CaptureSelect FcXP Ligand Leakage ELISA detects possible FcXP affinity ligand contamination of the purified product after use of CaptureSelect FcXP Affinity Matrix.

The FcXP affinity ligand is a 13-kD recombinant protein, expressed in yeast and covalently coupled to a chromatography matrix for high chemical stability and low leakage. During the chromatography process small amounts of the affinity ligand can leach off of the chromatography support and co-elute with the protein. This sensitive leakage ELISA detects affinity ligand contamination of less than 1 ng/mL. The assay is designed to minimize interference and to provide accurate quantitation even in the presence of the protein of interest. The CaptureSelect FcXP Ligand Leakage ELISA can be used as a tool to aid in optimal purification process development and in routine quality control of in-process streams as well as final product.

CaptureSelect™ IgG-Fc (rabbit) Affinity Matrix Thermo Scientific™

CaptureSelect IgG-Fc (Rabbit) Affinity Matrix has been specifically designed for the purification of rabbit IgG from recombinant and plasma sources. The ligand on the resin targets the Fc region of rabbit IgG antibodies independent of the light chain subclass.

Features of this affinity matrix include:
• Highly selective for rabbit IgG
• No binding to mouse, rat, human, bovine, horse, sheep, and goat IgG
• Complex growth media with fetal calf or horse serum can be applied
• Non-animal-derived

The high selectivity and yields obtained using CaptureSelect IgG-Fc (Rabbit) Affinity Matrix enables a robust and efficient purification process with excellent purity obtained in one step.

View other research scale antibody affinity resins ›

Free of animal components
Our CaptureSelect products are affinity ligands created by a proprietary technology based on camelid-derived single domain antibody fragments. The ligand is a 13-kDa single domain fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced by the yeast Saccharomyces cerevisiae in a production process free of animal components.

Main characteristics:

Matrix: agarose-based
Average particle size: 65 ± 10 µm
Ligand: CaptureSelect IgG-Fc (rabbit) affinity ligand
Ligand coupling method: epoxide
Binding capacity: >15 mg rabbit IgG/mL resin
Elution conditions: 1 M glycine, pH 3.0, or 20 mM acetic or citric acid, pH 3.0
Flow characteristics: 50–150 cm/h (up to 1 bar)
Formulation buffer: 20%(v/v) ethanol

CaptureSelect™ IgE Ligand Leakage ELISA Thermo Scientific™

The CaptureSelect IgE Ligand Leakage ELISA detects possible IgE affinity ligand contamination of the purified product after use of CaptureSelect IgE Affinity Matrix.

The CaptureSelect IgE affinity ligand is a 13-kD recombinant protein, expressed in yeast and covalently coupled to a chromatography matrix for high chemical stability and low leakage. During the chromatography process, small amounts of affinity ligand can leach off of the chromatography support and co-elute with the protein. This sensitive ELISA detects affinity ligand contamination of less than 1 ng/mL. The assay is designed to minimize interference and to provide accurate quantitation even in the presence of the protein of interest. The CaptureSelect IgE Ligand Leakage ELISA can be used as a tool to aid in optimal purification process development and in routine quality control of in-process streams as well as final product.

CaptureSelect™ IgG-Fc (ms) Affinity Matrix Thermo Scientific™

CaptureSelect™ IgG-Fc (ms) Affinity Matrix has been specifically designed to purify IgG from multiple species by high affinity binding to the Fc region of IgGs . Unlike protein G, gentle elution conditions can be used to recover the product. This affinity matrix can be used for purification of IgG from a broad range of species, such as human, mouse, rat, rabbit, cow, horse, and sheep. A complete list of species can be found in the product information sheet.

CaptureSelect™ C-tagXL MiniChrom Column Thermo Scientific™

CaptureSelect C-tagXL MiniChrom columns are designed for bench-scale resin screening, process development, and sample preparation. These pre-packed columns are ready to connect and have the following features:
• Dimensions of 1 mL (0.8 cm x 2 cm) and 5 mL (0.8 cm x 10 cm)
• Direct connection to standard chromatography systems
• Packed with CaptureSelect C-tagXL Affinity Matrix, offering high dynamic binding capacity and high resolution over a wide range of flow rates

The 1-mL columns can be used for quick screening of application feasibility and lab-scale purification on a convenient and easy-to-use pre-packed column. The 10-cm bed height of the 5-mL column allows initial process development on a bench scale.

CaptureSelect C-tagXL Affinity Matrix has a unique selectivity for a small 4-amino acid peptide tag (E-P-E-A, glutamic acid-proline-glutamic acid-alanine). CaptureSelect C-tagXL Affinity Matrix purifies the C-terminal tagged proteins with high affinity and selectivity, even in the presence of urea and guanidine HCl, from complex mixtures like cell culture harvests and periplasmic fractions in a one-step process. Mild elution conditions at neutral pH can be applied using magnesium chloride or propylene glycol, which ensures high-activity recoveries of pH-sensitive target proteins. The affinity resin recognizes the E-P-E-A tag sequence when fused either directly to the C-terminus of a protein or through a linker between the C-terminus and the E-P-E-A tag.

Features of this affinity matrix include:
• Mild elution, making it suitable for pH-sensitive proteins
• Binding of the tagged proteins under denaturing conditions (like dissolved inclusion bodies)
• Excellent scalability
• Non-animal-derived

View other biosimilars and recombinant protein affinity matrix formats ›

Free of animal components
CaptureSelect products contain affinity ligands created by a proprietary technology based on camelid-derived single-domain antibody fragments. The ligand is a 13-kDa single-domain fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced by the yeast Saccharomyces cerevisiae in a production process free of animal components.

Main characteristics:

Matrix: agarose-based, epoxide-activated
Average particle size: 65 ± 10 µm
Ligand: CaptureSelect C-tagXL affinity ligand
Ligand coupling method: epoxide coupling
Binding capacity: 400 nmol/mL resin depending on flow rate, column height, contact time
Elution conditions: Acidic: 20 mM citric acid or acetic acid, pH 3–4; 100 mM glycine buffer, pH 2–3. Neutral (pH 7.0–7.4): 20 mM Tris, 2.0 M MgCl2; 20 mM Tris, 1.0 M NaCl, 50% (v/v) propylene glycol; 20 mM Tris, 2 mM “S-E-P-E-A” peptide
Column dimensions: 1 mL (0.8 cm D x 2 cm L) and 5 mL (0.8 cm D x 10 cm L)
Flow characteristics: 75–150 cm/h (up to 2 bar)
Formulation buffer: 20%(v/v) ethanol

Pierce™ Protein Refolding Kit Thermo Scientific™

The Thermo Scientific Pierce Protein Refolding Kit includes high-purity reagents and detailed instructions for using a matrix strategy to determine optimal buffer conditions for refolding recombinant proteins that have been denatured and solubilized from inclusion bodies.

Features of the Protein Refolding Kit:

Robust—conditions and components examined are limited to those having the most significant and general utility as folding buffers
Convenient—three-level matrix design significantly reduces the amount of secondary optimization required and increases the ease of data interpretation
Adjustable matrix format—allows refolding experiments to be customized to the target protein; known positive and negative interactions between buffer components are addressed, minimizing unnecessary analyses
High-purity reagents—reagents are formulated using stringent standards so that consistent results are attained

The kit contains the essential reagents and complete strategy for determining optimal buffer conditions to refold denatured recombinant proteins to restore native structure and function. Nine base refolding buffers form a matrix that includes a range of strong and weak denaturant conditions for the suppression of protein aggregation. The supplied additives are used as additional matrix factors, depending on the protein type being refolded. Buffer components are examined at three concentration levels, allowing a wide spectrum of folding conditions to be tested within one experiment. The adjustable design allows matrix conditions to be tailored to the target protein, preventing sample waste and unnecessary analysis, while maximizing refolding yields. The Pierce Protein Refolding Kit is accompanied by a comprehensive Refolding Guide with details on isolating, solubilizing and purifying inclusion bodies; optimizing refolding conditions; and analyzing refolding yields.

Note: The following section is based on the original Previews article that reported on the development of the Pierce Protein Refolding Kit, which was originally called 'Pro-Matrix'.

Methods
Lysozyme was denatured overnight at 4°C in 8M GdnHCl, 10 mM DTT, 50 mM Tris, pH 8.0 at 20 mg/mL. Reduced glutathione, oxidized glutathione and DTT were added to refolding buffers as determined by the matrix layout (Table 3). All solutions were equilibrated to 4°C. Immediately before adding solubilized lysozyme to refolding buffers, the DTT was removed using a protein desalting spin column equilibrated in 8M GdnHCl, 50 mM Tris; pH 8.0. Lysozyme was then added to the refolding buffers at a final concentration of 1 mg/mL. This addition supplies 0.4M GdnHCl to the Base Refolding Buffers. Refolding was allowed to proceed for 18 hours at 4°C. Refolding yields were determined by measuring lysozyme activity with the EnzChek(tm) Lysozyme Assay Kit (Molecular Probes) using a Tecan(tm) SPECTRAFluor Plus System.

Results and Discussion
The basic protocol for protein refolding requires that inclusion bodies are first isolated, purified and then solubilized with a strong denaturant, such as guanidine hydrochloride (GdnHCl), to produce a completely unfolded protein. The solubilized protein is then diluted or dialyzed into a refolding buffer to reduce the denaturant concentration, allowing the protein to refold based on the information contained in its primary sequence. When using optimized conditions many proteins can be reliably refolded at concentrations >1 mg/ml. However, if the denaturant is removed and replaced with a non-optimized refolding buffer, protein aggregation strongly competes with renaturation and only minimal amounts of native protein are recovered. The degree of aggregation that occurs during refolding is largely dependent on protein concentration, concentration of strong and weak denaturants, pH, temperature, and the redox environment. Ionic strength, divalent cations, polymers and cofactors can also promote refolding of some proteins.

The results for refolding reduced and denatured lysozyme using the Pierce Protein Refolding Kit are reported in Table 3. For this example we treated lysozyme as if the presence of disulfide bonds in the native state was unknown. Reformation of native lysozyme was suppressed at the lowest and highest denaturant concentrations present within the protein refolding matrix (trial numbers 1, 2 and 9). Refolding was also suppressed by the presence of DTT (trial numbers 1, 6 and 8), showing the importance of reforming disulfide bonds in the folding of lysozyme. Highest lysozyme activity regained in the experiment was achieved in trial seven, which contained 1.4M GdnHCl, 0M L-arginine, 2 mM GSH: 0.4 mM GSSG, and represents more than 90% of the solubilized lysozyme being refolded.

HisPur™ Cobalt Chromatography Cartridges, 1 mL Thermo Scientific™

Thermo Scientific HisPur Cobalt Chromatography Cartridges contain a tetradentate chelating agarose resin charged with divalent cobalt (Co2+) for obtaining high-purity his-tagged proteins with no metal contamination.

Features of HisPur Cobalt Resin:

High purity—obtain more than 10 milligrams of pure His-tagged protein per milliliter of resin without optimizing imidazole washing conditions
Specificity—cobalt-chelate coordination core binds fewer host protein contaminants, resulting in lower background than nickel resins
Low metal leaching—no metal contamination in eluted histidine-tagged protein sample
Versatility—purify proteins under native or denaturing conditions; compatible with Thermo Scientific Pierce Cell Lysis Reagents and a variety of buffer additives
Cost effective—resin can be reused several times or discarded after single use
Flexibility—available as bulk resin, kits, predispensed columns, chromatography cartridges and 96-well filter plates

Compared to Ni-IDA and tetradentate Ni2+ chelate resins, HisPur Cobalt Resin binds histidine-tagged proteins with higher specificity (less off-target binding) and releases them with gentler conditions (lower concentrations of imidazole). Although Ni(2+) chelate resins achieve high protein yields, they bind somewhat indiscriminantly, resulting in suboptimal purity. Often, additional cleanup steps are required. By contrast, cobalt maximizes protein purity without sacrificing protein yield. HisPur Cobalt Resin binds fewer nonspecific proteins, displays less metal leaching and enables less stringent elution conditions than nickel resins.

HisPur Cobalt IMAC Resin specifically binds His-tagged recombinant protein that can be efficiently recovered by mild imidazole elution. Greater than 10 mg of His-tagged protein can be bound per milliliter of resin using a 28 kDa fusion protein expressed and purified from an E. coli lysate.

Various His-tagged proteins can be recovered in similar or higher purity compared to that obtained from other available nickel or cobalt IMAC resins using either a spin or gravity-flow column format. Purification performance of HisPur Cobalt Resin was benchmarked against IMAC resins from other suppliers using multiple His-tagged proteins of different sizes and expression levels. The results demonstrate that HisPur Cobalt Resin achieves maximal protein purity without sacrificing protein yield.

Available Formats:
Resin Slurries—crosslinked 6% beaded agarose; 10 mL, 100 mL, 500 mL bottles
Spin Columns—0.2 mL (microcentrifuge), 1 mL, and 3 mL columns
Purification Kits—complete kits in all three column sizes
Chromatography Cartridges—learn more and see HisPur Chromatography Cartridge data
96-well Spin Plates

Related Products
HisPur™ Cobalt Chromatography Cartridges, 5 mL
HisPur™ Cobalt Spin Columns, 1 mL
HisPur™ Cobalt Resin

UltraLink™ Hydrazide Resin Thermo Scientific™

Thermo Scientific UltraLink Hydrazide Resin is used to prepare affinity columns with antibodies or other glycolsylated proteins.

Features of UltraLink Hydrazide Resin:

High-capacity—immobilize 1 to 10 mg of oxidized antibody or other glycoprotein per milliliter of resin
Fast and efficient—immobilize in as few as 30 minutes; achieve at least 90% coupling of most glycoproteins in less than 4 hours (efficiency depends on the amount and type of glycosylation)
Specific immobilization—the hydrazide-activated UltraLink Resin conjugates only to purified glycoproteins whose sugar (e.g., sialic acid) groups have been gently oxidized with periodate
Stable attachment—resonance structure of the hydrazone bonds are sufficiently stable for multiple affinity purifications with one batch of prepared resin; no stabilization step is required
Preserves antibody function—immobilizes IgG via the Fc region, thereby keeping both antigen binding sites available for capturing target

Hydrazide-activated resin is used to prepare affinity columns with polyclonal antibodies, which typically have abundant carbohydrates (glycosylation) on their Fc portions. Any glycosylated proteins (even monoclonal antibodies) that contain periodate-oxidizable carbohydrate groups can be immobilized to make reusable affinity columns. Antibodies immobilized by hydrazide immobilization chemistry have unobstructed antigen-binding sites and optimal purification capability. Prepared columns with stable glycoproteins or antibodies can be regenerated and reused at least five times for affinity-purification without significant loss in binding capacity.

Applications:
• Purify antigens using covalently coupled antibodies
• Determine binding partners for a glycoprotein of interest
• Perform large-scale affinity purifications

UltraLink Hydrazide Resin is especially useful for glycoproteins, such as polyclonal antibodies, because it can allow molecules to be attached at a domain that will not interfere with active binding sites that are critical for the intended affinity purification. Carbohydrate moieties in glycoproteins contain common sugars with cis-diol groups that are easily oxidized with sodium meta-periodate to yield aldehydes. When incubated with hydrazide resin, these aldehyde groups react with the hydrazide groups on the resin to form stable, covalent bonds. This reaction is enhanced by the Thermo Scientific GlycoLink Coupling Catalyst, increasing coupling efficiency and decreasing incubation times. The entire coupling reaction occurs in 2 to 4 hours in a simple non-amine buffer containing the catalyst. Coupling efficiencies with antibodies and typical glycoproteins are generally greater than 85%, resulting in 1 to 10 mg of immobilized protein per milliliter of Thermo Scientific UltraLink Hydrazide Resin.

More Product Data
Improved immobilization and conjugation of glycoproteins

Related Products
GlycoLink™ Immobilization Kit
GlycoLink™ Micro Immobilization Kit

CaptureSelect™ Biotin Anti-Free LC-kappa (Human) Conjugate Thermo Scientific™

CaptureSelect™ Biotin Anti-Free LC-kappa (Hu) Conjugate consists of a 13 kDa llama antibody fragment (affinity ligand) that binds highly selectively to free human kappa light chains and dimers thereof. The functionality is based on recognition of an epitope on the human CL-kappa domain that is masked in intact antibodies and Fab fragments. The affinity ligand is chemically conjugated to biotin via an appropriate spacer that retains the binding reactivity of the ligand when used in combination with streptavidin-based conjugates or streptavidin pre-coated surfaces.

The CaptureSelect™ Biotin Anti-Free LC-kappa (Hu) Conjugate allows you to:

Detect, quantitate, and characterize free human kappa light chains in (crude) antibody preps
Avoid cross-binding with human kappa light chains present in intact and properly folded antibodies and Fab fragments of any human antibody isotype such as IgG, IgA, IgM, IgD, and IgE

Applications for CaptureSelect™ Biotin Anti-Free LC-kappa (Hu) Conjugate include ELISA, Western blot, Gyrolab™-based immunoassays, and label-free detection platforms such as those based on surface plasmon resonance (Biacore™ and IBIS-MX96 systems) and bio-layer interferometry (ForteBio™ Octet™ systems).

CaptureSelect™ IgG3 (Hu) Affinity Matrix Thermo Scientific™

CaptureSelect™ IgG3 (Hu) Affinity Matrix can be used for the purification and isolation of IgG3, without cross-reactivity to other immunoglobulins, like other human IgG subclasses, IgA, and IgM, from complex sources like plasma, serum, and cell culture supernatants.

PichiaPink™ Secreted Protein Kit Invitrogen™

The PichiaPink™ Secreted Protein Expression Kit is a new recombinant protein expression system based on the yeast Pichia pastoris. The PichiaPink™ Secreted Protein Expression Kit gives you convenient and cost-efficient bioproduction from shake flask to 1000-liter production of your recombinant protein. The PichiaPink™ Secreted Protein Expression Kit comes with our new Pichia strains, the pPINK-HC and pPINK-LC vectors, and secretion signal sequences for production of your protein.
  Get started with secreted protein production in Pichia pastoris.
•   Screen your Pichia transformants rapidly by auxotrophy and color selection.
•   Reduce protein degradation with protease-deficient Pichia pastoris strains.

The PichiaPink™ Secreted Protein Expression Kit comes with:
•   PichiaPink™ Secreted Protein Vector Kit (Cat. No. A11153)
•   PichiaPink™ Expression Strain Set (Cat. No. A11154)
•   PichiaPink™ Media Kit (Cat. No. A11156)

A New Way to Screen Pichia Transformants
The PichiaPink™ Secreted Protein Expression Kit uses adenine auxotrophy to select for Pichia transformants. The PichiaPink™ strains have the ade2 genotype and require complementation with the ADE2 gene to grow without adenine. Use of an auxotrophic marker also makes it easier to maintain transformants. No antibiotics are required to maintain your transformants.

In addition, you can screen for Pichia transformants by color. The untransformed PichiaPink™ strains appear as pink colonies while transformed PichiaPink™ strains appear as white colonies (Fig 1).

Secrete Your Proteins with the pPINKα-HC Vector

The PichiaPink™ Secreted Protein Expression Kit comes with the pPINKα-HC vector. The pPINKα-HC vector has the α-mating factor signal sequence built-in and allows your protein to be secreted into the media. Secreting your protein into the media makes harvesting and purifying your protein easier. The pPINKα-HC vector is built around the ADE2 gene for complementing the ade2-deficient Pichia strains. This vector uses the methanol-induced AOX1 promoter to express your protein. Further, the pPINKα-HC vector is designed to be integrated at high-copy-number in your Pichia transformants.

The PichiaPink™ Secreted Protein Vector Kit (Cat. No. A11153) can be ordered separately to refill the PichiaPink™ Secreted Protein Expression Kit.

Keep Your Proteins Intact
The PichiaPink™ Secreted Protein Expression Kit comes with the PichiaPink™ Expression Strain Set. This set contains the 4 Pichia pastoris strains for use with the PichiaPink™ Yeast Expression System. All 4 strains have the ade2 genotype. In addition, 3 strains include knockouts in 2 protease genes. Protease-deficient strains reduce the need for protease inhibitors when growing your cells and improve the yield of your protein. Test a single protease knockout or the double protease knockout to find the one that works best for you.

You can order the PichiaPink™ Expression Strain Set (Cat. No. A11154) separately to refill the PichiaPink™ Secreted Protein Expression Kit .

Media Pouches to Get You Started

The PichiaPink™ Media Kit is a set of convenient prepackaged media pouches for use with the PichiaPink™ Secreted Protein Expression Kit. The PichiaPink™ Media Kit contains media needed to grow PichiaPink™ strains from frozen stocks to cultures ready for transformation and selection. You can order the PichiaPink™ Media Kit (Cat. No. A11156) separately to refill the PichiaPink™ Secreted Protein Expression Kit.

Why Choose the PichiaPink™ Yeast Expression System?
The PichiaPink™ Yeast Expression System is based on the yeast Pichia pastoris. Advantages of Pichia pastoris include rapid growth, well-defined genetic background, simple media formulation, and easy handling. For over 30 years, Pichia pastoris has been used by labs around the world for producing hundreds of different proteins from many species including human (Ref 1, 2). The PichiaPink™ Yeast Expression System allows convenient and cost-effective protein production from small to large scales.

For information on obtaining a commercial-use license for the PichiaPink™Yeast Expression System, please inquire at outlicensing@lifetech.com.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Related Links
Learn more about the PichiaPink™ Yeast Expression System.
Learn more about our other protein expression systems.

References

1. Cereghino JL, Cregg JM. Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol Rev. 2000 Jan;24(1):45-66. [PubMed]
2. Cereghino GP, Cereghino JL, Ilgen C, Cregg JM. Production of recombinant proteins in fermenter cultures of the yeast Pichia pastoris. Curr Opin Biotechnol. 2002 Aug;13(4):329-32. [PubMed]
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