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CaptureSelect™ IgG-CH1 EvolveD™ Column Thermo Scientific™

CaptureSelect IgG-CH1 EvolveD columns are ready-to-use columns designed specifically for use in biopharmaceutical applications. These pre-packed columns are suitable for cGMP manufacturing and have the following features:
• Prepared and packed in a classified ISO7 environment
• An entirely non-metallic flow path
• Qualified in terms of performance and pressure flow characteristics
• Suitable for direct connection to standard chromatography systems
• Available Regulatory Support File
• Packed with CaptureSelect IgG-CH1 Affinity Matrix, offering high dynamic binding capacity and high resolution over a wide range of flow rates

Please note that CaptureSelect EvolveD affinity columns are made to order. They are custom manufactured two-at-a-time, so are offered only in quantities that are a multiple of two. For detailed information on the resin, please see the CaptureSelect EvolveD column manual below.

Operating and technical specifications
Column type10-cm bed height20-cm bed height
Column diameter (cm)7102071020
Column length (cm)101010202020
Cross-sectional area (cm2)38.578.5314.238.578.5314.2
Column volume (mL)385785314277015706284

• Pressure rating (bar/psi): 4/58
• Operating temperature: 2–30°C, dependent on the resin packed
• Bed support size: 8 µm
• Inlet/outlet internal diameter: 3 mm or 6 mm depending on the column diameter
• Inlet/outlet port connections: 25-mm TC sanitary fitting

View our comprehensive column overview › (Select the Antibody Purification tab.)

CaptureSelect IgG-CH1 Affinity Matrix recognizes all IgG subclasses (1, 2, 3, and 4) independent of the light chain subclass (Kappa and Lambda).

Features of this affinity matrix include:
• Purification of IgG, Fab, and Fab2 fragments directly from complex source materials in a single step with high purity and yield
• No co-purification of overexpressed free lights chains due to heavy chain binding
• Human specific, no binding to bovine antibodies
• Non–animal-derived

View other antibody affinity matrices ›

Free of animal components
CaptureSelect products are affinity ligands created with proprietary technology based on camelid-derived single-domain antibody fragments. The IgG-CH1 ligand is a 14-kDa fragment comprising the three complementarity determining regions (CDRs) that form the antigen binding domain. The ligand is efficiently produced by the yeast Saccharomyces cerevisiae in a production process free of animal components.

Main characteristics:

Matrix: agarose-based, epoxide-activated
Average particle size: 65 ± 10 µm
Ligand: CaptureSelect IgG-CH1 affinity ligand
Ligand coupling method: aldehyde coupling via NH2 residues of the ligand
Binding capacity: 25–30 g IgG/L resin depending on flow rate, column height, and contact time
Elution conditions: 20 mM citric acid, 150 mM NaCl, pH 3.5
Max. operation pressure: 2 bar
Formulation buffer: 20% (v/v) ethanol

CaptureSelect™ KappaXL EvolveD™ Column Thermo Scientific™

CaptureSelect KappaXL EvolveD columns are ready-to-use columns designed specifically for use in biopharmaceutical applications. These pre-packed columns are suitable for cGMP manufacturing and have the following features:
• Prepared and packed in a classified ISO7 environment
• An entirely non-metallic flow path
• Qualified in terms of performance and pressure flow characteristics
• Suitable for direct connection to standard chromatography systems
• Available Regulatory Support File
• Packed with CaptureSelect KappaXL Affinity Matrix, offering high dynamic binding capacity and high resolution over a wide range of flow rates

Please note that CaptureSelect EvolveD affinity columns are made to order. They are custom manufactured two-at-a-time, so are offered only in quantities that are a multiple of two. For detailed information on the resin, please see the CaptureSelect EvolveD column manual below.

Operating and technical specifications
Column type10-cm bed height20-cm bed height
Column diameter (cm)7102071020
Column length (cm)101010202020
Cross-sectional area (cm2)38.578.5314.238.578.5314.2
Column volume (mL)385785314277015706284

• Pressure rating (bar/psi): 4/58
• Operating temperature: 2–30°C, dependent on the resin packed
• Bed support size: 8 µm
• Inlet/outlet internal diameter: 3 mm or 6 mm depending on the column diameter
• Inlet/outlet port connections: 25-mm TC sanitary fitting

View our comprehensive column overview › (Select the Antibody Purification tab.)

CaptureSelect KappaXL Affinity Matrix is designed for the purification of recombinant human Ig’s (including IgA, IgM, IgD, and IgE) containing a Kappa light chain and their Fab fragments.

Features of this affinity matrix include:
• Purification of Kappa monoclonal antibodies, Fab-, and Fab2 fragments directly from complex source materials in a single step with high purity and yield
• Mild elution for Fab fragments and antibodies
• Human specific, no binding to bovine antibodies
• Non-animal derived

View other antibody affinity matrices ›

Free of animal components
CaptureSelect products are affinity ligands created with proprietary technology based on camelid-derived single-domain antibody fragments. The KappaXL ligand is a 13-kDa fragment comprising the three complementarity determining regions (CDRs) that form the antigen binding domain. The ligand is efficiently produced by the yeast Saccharomyces cerevisiae in a production process free of animal components.

Main characteristics:

Matrix: agarose-based, epoxide-activated
Average particle size: 65 ± 10 µm
Ligand: CaptureSelect KappaXL affinity ligand
Ligand coupling method: aldehyde coupling via NH2 residues of the ligand
Binding capacity: 29–38 g IgG/L resin depending on flow rate, column height, and contact time
Elution conditions: 20 mM citric acid, pH 3.5
Max. operation pressure: 2 bar
Formulation buffer: 20% (v/v) ethanol

CaptureSelect™ HCG EvolveD™ Column Thermo Scientific™

CaptureSelect HCG EvolveD columns are ready-to-use columns designed specifically for use in biopharmaceutical applications. These pre-packed columns are suitable for cGMP manufacturing and have the following features:
• Prepared and packed in a classified ISO7 environment
• An entirely non-metallic flow path
• Qualified in terms of performance and pressure flow characteristics
• Suitable for direct connection to standard chromatography systems
• Available Regulatory Support File
• Packed with CaptureSelect HCG Affinity Matrix, offering high dynamic binding capacity and high resolution over a wide range of flow rates

Please note that CaptureSelect EvolveD affinity columns are made to order. They are custom manufactured two-at-a-time, so are offered only in quantities that are a multiple of two. For detailed information on the resin, please see the CaptureSelect EvolveD column manual below.

Operating and technical specifications
Column type10-cm bed height20-cm bed height
Column diameter (cm)7102071020
Column length (cm)101010202020
Cross-sectional area (cm2)38.578.5314.238.578.5314.2
Column volume (mL)385785314277015706284

• Pressure rating (bar/psi): 4/58
• Operating temperature: 2–30°C, dependent on the resin packed
• Bed support size: 8 µm
• Inlet/outlet internal diameter: 3 mm or 6 mm depending on the column diameter
• Inlet/outlet port connections: 25-mm TC sanitary fitting

View our comprehensive column overview › (Select the Antibody Purification tab.)

CaptureSelect HCG Affinity Matrix is designed for the purification of human chorionic gonadotropin from recombinant sources.

Features of this affinity matrix include:
• Selective for hCG, with cross-binding to LH and FSH due to the identical alpha chain subunit that is recognized by the ligand
• Enables efficient purification of recombinant gonadotropins with high yields and purity
• Human specific
• Non-animal derived

View other biosimilars and recombinant proteins affinity matrices ›

Free of animal components
CaptureSelect products are affinity ligands created with proprietary technology based on camelid-derived single-domain antibody fragments. The HCG ligand is a 13-kDa fragment comprising the three complementarity determining regions (CDRs) that form the antigen binding domain. The ligand is efficiently produced by the yeast Saccharomyces cerevisiae in a production process free of animal components.

Main characteristics:

Matrix: agarose-based, epoxide-activated
Average particle size: 65 ± 10 µm
Ligand: CaptureSelect HCG affinity ligand
Ligand coupling method: aldehyde coupling via NH2 residues of the ligand
Binding capacity: ~4 mg HCG/mL resin depending on flow rate, column height, and contact time
Elution conditions: 50 mM citric acid, pH 3.0
Max. operation pressure: 2 bar
Formulation buffer: 20% (v/v) ethanol

CaptureSelect™ MultiSpecies Albumin Depletion Product Thermo Scientific™

This is one of a group of CaptureSelect™ Proteomics Toolbox™ affinity resins which are unique affinity resins developed for the depletion of proteins from human plasma or serum samples. These affinity ligands are immobilized on a high capacity agarose support for easy, lower-pressure protein depletion or sample prep applications.

CaptureSelect™ Biotin Anti-C3 Conjugate Thermo Scientific™

CaptureSelect™ Biotin Anti-C3 Conjugate consists of a 13 kDa camelid antibody fragment (affinity ligand) that specifically binds with high affinity and selectivity to human complement component 3 (C3). The affinity ligand is chemically conjugated to biotin via an appropriate spacer that retains the binding reactivity of the ligand when used in combination with streptavidin-based conjugates or streptavidin pre-coated surfaces. This conjugate allows you to detect, quantitate, and characterize native and recombinant human C3.

Applications for CaptureSelect Biotin Anti-C3 Conjugate include ELISA, immunoprecipitation with streptavidin-coupled Dynabeads™, MSIA Streptavidin D.A.R.T.’S, Gyrolab™-based immunoassays, and label-free detection platforms such as those based on surface plasmon resonance (Biacore™ and IBIS-MX96 systems) and bio-layer interferometry (ForteBio™ Octet™ systems).

CaptureSelect™ IgE EvolveD™ Column Thermo Scientific™

CaptureSelect IgE EvolveD columns are ready-to-use columns designed specifically for use in biopharmaceutical applications. These pre-packed columns are suitable for cGMP manufacturing and have the following features:
• Prepared and packed in a classified ISO7 environment
• An entirely non-metallic flow path
• Qualified in terms of performance and pressure flow characteristics
• Suitable for direct connection to standard chromatography systems
• Available Regulatory Support File
• Packed with CaptureSelect IgE Affinity Matrix, offering high dynamic binding capacity and high resolution over a wide range of flow rates

Please note that CaptureSelect EvolveD affinity columns are made to order. They are custom manufactured two-at-a-time, so are offered only in quantities that are a multiple of two. For detailed information on the resin, please see the CaptureSelect EvolveD column manual below.

Operating and technical specifications
Column type10-cm bed height20-cm bed height
Column diameter (cm)7102071020
Column length (cm)101010202020
Cross-sectional area (cm2)38.578.5314.238.578.5314.2
Column volume (mL)385785314277015706284

• Pressure rating (bar/psi): 4/58
• Operating temperature: 2–30°C, dependent on the resin packed
• Bed support size: 8 µm
• Inlet/outlet internal diameter: 3 mm or 6 mm depending on the column diameter
• Inlet/outlet port connections: 25-mm TC sanitary fitting

View our comprehensive column overview › (Select the Antibody Purification tab.)

CaptureSelect IgE Affinity Matrix is designed for the purification of human IgE (immunoglobuline E) from recombinant and plasma sources.

Features of this affinity matrix include:
• Purification of human IgE, binding different recombinant IgE forms
• IgE specific, no cross-binding to other human immunoglobulins
• Efficient removal of host cell proteins (HCP) and IgE aggregates
• Efficient elution at pH 3.0–3.5
• Excellent scalability
• Non-animal derived

View other biosimilars and recombinant proteins affinity matrices ›

Free of animal components
CaptureSelect products are affinity ligands created with proprietary technology based on camelid-derived single-domain antibody fragments. The IgE ligand is a 13-kDa fragment comprising the three complementarity determining regions (CDRs) that form the antigen binding domain. The ligand is efficiently produced by the yeast Saccharomyces cerevisiae in a production process free of animal components.

Main characteristics:

Matrix: agarose-based, epoxide-activated
Average particle size: 65 ± 10 µm
Ligand: CaptureSelect IgE affinity ligand
Ligand coupling method: epoxide coupling
Binding capacity: >15 g IgE/liter resin depending on flow rate, column height, and contact time
Elution conditions: 20 mM sodium citrate, pH 3.0
Max. operation pressure: 2 bar
Formulation buffer: 20% (v/v) ethanol

Neucode™ Lysine-202 Thermo Scientific™

Thermo Scientific NeuCode amino acids augment the level of multiplexing achievable for the metabolic labeling of proteins for mass spectrometry analysis. NeuCode Lysine-202 (13C2 15N2 L-Lysine-2HCl) may also be used with traditional SILAC to improve flexibility of multiplexing options or to reduce complexity of analysis.

General features of NeuCode SILAC labeling:
• Labeling efficiency—100% label incorporation into proteins of living cells without toxicity
• Compatible—may be multiplexed with existing SILAC amino acids
• Time-saving—not necessary to label to 100% incorporation if only using heavy amino acids
• High-quality supplements—heavy amino acids with >98% isotope purity

Stable isotope labeling with amino acids in cell culture (SILAC) is a powerful method to identify and quantify relative differential changes in complex protein samples. NeuCode metabolic labeling is similar to SILAC, but differs in that the labeling only utilizes heavy amino acids. The increased multiplexing capability of NeuCode amino acids is possible through the use of mass defects from extra neutrons in the stable isotopes. These small mass differences may be resolved on high resolution mass spectrometers (Thermo Scientific™ Orbitrap™ Elite™, Orbitrap™ Fusion™ Tribrid™, and Orbitrap™ Fusion™ Lumos™ Tribrid™ mass spectrometers). Use of only heavy amino acids eliminates the need for 100% incorporation of amino acids used for SILAC (both heavy and light), and may be especially useful for studies with primary cells.

NeuCode amino acids are used together with specialized cell culture media that are deficient in essential amino acids. Heavy L-lysine is used for SILAC analysis of peptides that have been digested with trypsin or LysC. NeuCode Lysine-202 may be used with 4,4,5,5-D4 L-Lysine for NeuCode duplex experiments and may be combined with light lysine and 13C6 15N2 L-Lysine-2HCl for SILAC three-plex experiments.

Related products
L-Lysine-2HCl, 4,4,5,5-D4
13C6 15N2 L-Lysine-2HCl
NeuCode Lysine-080

NeuCode is a trademark of WARF.

Ni-NTA Agarose Invitrogen™

Ni-NTA Agarose is a nickel-charged affinity resin that can be used to purify recombinant proteins containing a polyhistidine (6xHis) sequence. Proteins bound to the resin may be eluted with either low pH buffer or by competition with imidazole or histidine. One-step purification can be performed under both native and denaturing conditions. Ni-NTA uses the chelating ligand nitrilotriacetic acid (NTA) coupled to a cross-linked 6% agarose resin that is suitable for use in batch and gravity flow applications.

HisPur™ Ni-NTA Spin Purification Kit, 1 mL Thermo Scientific™

The Thermo Scientific HisPur Ni-NTA Spin Purification Kit contains 1-ml HisPur Ni-NTA spin columns, as well as buffers required to purify polyhistidine-tagged proteins. HisPur Ni-NTA resin is a high-capacity, high-performance nickel-IMAC resin for routine affinity purification of His-tagged fusion proteins.

Features of HisPur Ni-NTA Resin:

High capacity—bind up to 60 mg of 6xHis-tagged protein per milliliter of resin
Versatile—purify proteins using native or denaturing conditions
Compatible—use with Thermo Scientific Cell Lysis Reagents and a variety of buffer additives
Cost-effective—reuse the same batch of resin at least five times
Easy to use—pre-formulated buffers available for kit formats
Flexible—available in multiple formats including bulk resin, spin columns, chromatography cartridges and 96-well filter plates

The specially prepared support consists of beaded agarose derivatized with the nitrilotriacetic acid (NTA) chelation moiety and loaded with divalent nickel ions (Ni2+). The immobilized metal affinity chromatography (IMAC) resin provides exceptional binding capacity and performance for recombinant His-tagged protein purification.

The expression and purification of recombinant proteins is central to protein regulation, structure and function studies. The majority of recombinant proteins are expressed as fusions with short affinity tags, the most popular being the polyhistidine (6xHis) tag. The method used to purify recombinant His-tagged proteins is immobilized metal affinity chromatography (IMAC), consisting of chelating resins charged with either nickel or cobalt ions that coordinate with the histidine side chains.

HisPur Ni-NTA Resin effectively purifies high levels of overexpressed His-tagged fusion proteins from bacterial lysates, such as those that are attained with Thermo Scientific B-PER Bacterial Protein Extraction Reagents. The resin performs well in batch-binding and spin-column procedures at a variety of scales. Performance equals or exceeds popular Ni-NTA resins from other suppliers. HisPur Ni-NTA Resin is a high-quality, stable and resilient affinity support. Tests confirm that no decrease in performance occurs after at least five repeated uses. These data indicated that the resin is highly resistant to structural degradation or nickel ion leaching during normal use.

HisPur Ni-NTA Resin and HisPur Cobalt Resin are alternative forms of IMAC, the former using nickel and the latter using cobalt as the chelated metal ion responsible for His-tag binding. Ni-NTA resins have been the most common IMAC resin choice for 6xHis-tag protein purifications because of the four metal-binding sites on the chelate, which enables high-protein binding and low-metal ion leaching. Cobalt binds less strongly and therefore more discriminantly, enabling greater purity but usually less yield in His-tagged protein purification.

Available Formats:
Resin Slurries—crosslinked 6% beaded agarose; 10 mL, 100 mL, 500 mL bottles
Spin Columns—0.2 mL (microcentrifuge), 1 mL, 3 mL columns
Purification Kits—complete kits in all three column sizes
Chromatography Cartridges—learn more and see HisPur Chromatography Cartridge data
96-well Spin Plates

Related Products
HisPur™ Ni-NTA Resin
HisPur™ Ni-NTA Spin Columns, 1 mL

Pierce™ Anti-DYKDDDDK Magnetic Agarose Thermo Scientific™

Thermo Scientific Pierce Anti-DYKDDDDK Magnetic Agarose provides a fast, convenient method for purification and immunoprecipitation (IP) of DYKDDDDK-tagged proteins from in vitro protein expression systems, bacteria, yeast, and mammalian cells. The amino acid sequence DYKDDDDK, commonly known as 'FLAG', is recognized by a high-affinity rat monoclonal antibody (clone L5) that is covalently attached to a magnetite-embedded agarose core particle.

For protein purification, the magnetic agarose is added to a sample containing DYKDDDDK-tagged proteins with the tag on either the N- or the C-terminus. Captured proteins are then magnetically separated from the supernatant, and non-specifically bound proteins can be washed away before dissociating bound DYKDDDDK-tagged proteins with elution buffer. The magnetic agarose is removed from the solution using a magnetic stand or an instrument such as the KingFisher Flex Magnetic Particle Processor. Automated instruments are especially useful for higher throughput purifications and screening of purification conditions.

Features include:
Specific—unique base beads and highly specific antibody minimizes off-target binding (low non-specific binding)
High purity—optimized bind-wash-elute protocol enables high purity
High yield—special antibody conjugation method enables high yield
Rapid—entire purification protocol typically takes less than 40 mins
Economical—purification protocol allows multiple reuses
Versatile—beads are compatible with manual and automated workflows (e.g., KingFisher instruments)

Characteristics of Pierce Anti-DYKDDDDK Magnetic Agarose:

Composition: anti-DYKDDDDK antibody covalently attached to a magnetic, highly crosslinked agarose support
Magnetization: ferrimagnetic with low remanence
Bead size: 10–40 µm
Bead concentration: 25% slurry in phosphate buffered saline, 0.01% Tween-20 detergent, 0.02% sodium azide, pH 7.2
Binding capacity: ≥3.2 mg DYKDDDDK-tGFP-His protein (~32 kDa)/mL settled beads

CaptureSelect™ AAV9 Ligand Leakage ELISA Kit Thermo Scientific™

The CaptureSelect™ AAV9 Ligand Leakage ELISA detects affinity ligand contamination of antibodies or antibody fragments purified using POROS™ CaptureSelect™ AAV9 Affinity Resin. The ligand, a 13 kD recombinant protein, is coupled by multipoint attachment through stable amide bonds to give high chemical stability and low leakage. Even when covalently attached, small amounts of the affinity ligand can leach off of the chromatography support and co-elute with the protein. This sensitive ELISA detects affinity ligand contamination of less than 1 ng/mL. The assay is designed to minimize interference and to provide accurate quantitation even in the presence of the protein of interest. The CaptureSelect™ AAV9 Ligand Leakage ELISA can be used as a tool to aid in optimal purification process development and in routine quality control of in-process streams as well as final product.

Pierce™ Agarose ChIP Kit Thermo Scientific™

The Thermo Scientific Pierce Agarose ChIP Kit provides a complete set of reagents and a simple, fast and reproducible protocol to perform chromatin immunoprecipitation (ChIP) assays.

Features of the Agarose ChIP Kit:

• Simple and fast ChIP assay protocol (< 8 hours)
• Highly efficient isolation and lysis of nuclei
• Easy and reproducible enzymatic digestion
• Low-background and high-binding capacity Protein A/G agarose resin
• Highly specific positive controls included: RNA polymerase II antibody and GAPDH PCR primers
• Fast and reproducible spin-column format
• High-recovery DNA purification
• ChIP-validated antibodies available

The Pierce Agarose ChIP Kit contains sufficient reagents to perform 30 ChIP assays with appropriate controls using an optimized protocol and a convenient spin-column format. ChIP-validated and quality-guaranteed antibodies are also available for use the the Pierce Agarose ChIP Kit. Because antibodies that work for western blotting may not function in ChIP assays, we have assembled our most popular ChIP-validated monoclonal and polyclonal antibodies here to help you move from experimental setup to results more quickly.

The strength of the ChIP assay is its ability to capture a snapshot of specific protein-DNA interactions as they occur in living cells, and then quantitate the interactions using standard or quantitive PCR. A successful ChIP assays requires a number of critical steps to be carried out (crosslinking, chromatin preparation, immunoprecipitation) prior to detecting the target genomic DNA sequence. Individually, each step in the ChIP assay protocol can be time consuming to optimize. The Pierce Agarose ChIP Assay Kit simplifies the process, making accurate and reproducible results easier to obtain.

To perform ChIP assays with the Pierce Agarose ChIP Kit, protein-DNA complexes are stabilized and then extracted. In vivo crosslinking is traditionally achieved with formaldehyde. Crosslinking performed directly in cells locks in the protein-DNA complexes, trapping these unstable and sometimes transient interactions. To lyse, extract and solubilize the crosslinked complexes, the ChIP assay kit includes the Thermo Scientific Chromatin Prep Module (also sold separately, Part No. 26158). Together, this complete ChIP assay kit provides a simple, reliable and convenient means for isolating chromatin-bound DNA and enriching samples for the proteins of interest with less than 15% contamination from other cellular compartments - without a Dounce homogenizer.

Related Products
Pierce™ Chromatin Prep Module
Pierce™ ChIP-Grade Protein A/G Plus Agarose
Proteinase K

POROS™ 50 D Weak Anion Exchange Resin Thermo Scientific™

Applied Biosystems™ POROS™ Weak Anion Exchange Media. POROS™ 50 micron bulk resins is available for low pressure applications, including large scale downstream bioprocessing.

POROS™ High Performance Chromatography™ Media and Pre-Packed Columns

A high performance chromatography resins for analytical to process scale separations.• Higher productivity: High throughput and high dynamic capacity associated with High Performance Chromatography™
• Chemical stability: Allows aggressive cleaning and sanitization
• Enhanced biomolecule access: Provided via large pores, ranging between 500-10000 Å
• Polystyrenedivinylbenzene particles: Yield a robust, easily packable matrix
• Develop better separations methods in a shorter time frame: The speed of High Performance Chromatography technology reduces weeks of experimentation to only a few hours of work, so you have plenty of time to explore all the variables of your separation

Reduce time-consuming sample prep

You can replace many sample preparation steps with high speed High Performance Chromatography technology. For instance, dialysis of large volumes of material can be replaced by high flow rate processing of the dilute sample. Elution in a small volume of new buffer accomplishes both buffer exchange and concentration.

Create novel assays for more efficient analysis

High Performance Chromatography technology is not limited to standard modes of separation. Both enzymes and affinity ligands can be immobilized on POROS media. By combining rapid on-column protein digestions with rapid on-column immunoassays and chromatographic separations, you can create entirely new assays with unlimited potential.

High capacity, high resolution, high speed
In contrast to conventional chromatography media, POROS™ High Performance Chromatography™ resins particles, are engineered to have two discreet classes of pores. Large "throughpores" allow convection flow to occur through the particles themselves, quickly carrying sample molecules to short "diffusive" pores inside. By reducing the distance over which diffusion needs to occur, the time required for sample molecules to interact with interior binding sites is also reduced. Diffusion is no longer limiting and flow rates can be dramatically increased - without compromising resolution or capacity. Separations can be achieved at 1,000 to 5,000 cm⁄hr compared to 50 to 360 cm⁄hr for conventional media.

HisPur™ Ni-NTA Resin Thermo Scientific™

Thermo Scientific HisPur Ni-NTA Resin is a high-capacity, high-performance nickel-IMAC resin for routine affinity purification of His-tagged fusion proteins.

Features of HisPur Ni-NTA Resin:

High capacity—bind up to 60 mg of 6xHis-tagged protein per milliliter of resin
Versatile—purify proteins using native or denaturing conditions
Compatible—use with Thermo Scientific Cell Lysis Reagents and a variety of buffer additives
Cost-effective—reuse the same batch of resin at least five times
Easy to use—pre-formulated buffers available for kit formats
Flexible—available in multiple formats including bulk resin, spin columns, chromatography cartridges and 96-well filter plates

The specially prepared support consists of beaded agarose derivatized with the nitrilotriacetic acid (NTA) chelation moiety and loaded with divalent nickel ions (Ni2+). The immobilized metal affinity chromatography (IMAC) resin provides exceptional binding capacity and performance for recombinant His-tagged protein purification.

The expression and purification of recombinant proteins is central to protein regulation, structure and function studies. The majority of recombinant proteins are expressed as fusions with short affinity tags, the most popular being the polyhistidine (6xHis) tag. The method used to purify recombinant His-tagged proteins is immobilized metal affinity chromatography (IMAC), consisting of chelating resins charged with either nickel or cobalt ions that coordinate with the histidine side chains.

HisPur Ni-NTA Resin effectively purifies high levels of overexpressed His-tagged fusion proteins from bacterial lysates, such as those that are attained with Thermo Scientific B-PER Bacterial Protein Extraction Reagents. The resin performs well in batch-binding and spin-column procedures at a variety of scales. Performance equals or exceeds popular Ni-NTA resins from other suppliers. HisPur Ni-NTA Resin is a high-quality, stable and resilient affinity support. Tests confirm that no decrease in performance occurs after at least five repeated uses. These data indicated that the resin is highly resistant to structural degradation or nickel ion leaching during normal use.

HisPur Ni-NTA Resin and HisPur Cobalt Resin are alternative forms of IMAC, the former using nickel and the latter using cobalt as the chelated metal ion responsible for His-tag binding. Ni-NTA resins have been the most common IMAC resin choice for 6xHis-tag protein purifications because of the four metal-binding sites on the chelate, which enables high-protein binding and low-metal ion leaching. Cobalt binds less strongly and therefore more discriminantly, enabling greater purity but usually less yield in His-tagged protein purification.

Available Formats:
Resin Slurries—crosslinked 6% beaded agarose; 10 mL, 100 mL, 500 mL bottles
Spin Columns—0.2 mL (microcentrifuge), 1 mL, 3 mL columns
Purification Kits—complete kits in all three column sizes
Chromatography Cartridges—learn more and see HisPur Chromatography Cartridge data
96-well Spin Plates

Related Products
HisPur™ Ni-NTA Spin Columns, 0.2 mL
HisPur™ Ni-NTA Spin Columns, 1 mL
HisPur™ Ni-NTA Spin Columns, 3 mL
HisPur™ Ni-NTA Chromatography Cartridges, 1 mL
HisPur™ Ni-NTA Superflow Agarose

CaptureSelect™ IgG-Fc (ms) Affinity Matrix Thermo Scientific™

CaptureSelect™ IgG-Fc (ms) Affinity Matrix has been specifically designed to purify IgG from multiple species by high affinity binding to the Fc region of IgGs . Unlike protein G, gentle elution conditions can be used to recover the product. This affinity matrix can be used for purification of IgG from a broad range of species, such as human, mouse, rat, rabbit, cow, horse, and sheep. A complete list of species can be found in the product information sheet.
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