Shop All Spin Columns and Gravity-flow Columns

Pierce™ Spin Columns - Snap Cap Thermo Scientific™

Thermo Scientific Pierce Snap Cap Spin Columns are convenient tools for manipulating small volumes of affinity supports (20 to 500 µL) for protein purification.

Features of Snap Cap Spin Columns:

Formulation: polypropylene
Column volume: 1 mL
Resin volume: 20 to 500µL
Filter type: Polyethylene filter, ~30 µm pore size
Cap: Attached snap-cap (no cap on collection tube); press-on bottom caps

Simply add the affinity resin and sample to one of the spin columns, then use a microcentrifuge to efficiently wash away contaminants and elute your purified sample without losing any resin in the process. Spin columns allow you to affinity purify more protein in less time.

Applications:
• Affinity purification or affinity chromatography
• Immunodepletion
• Immunoprecipitation (IP)
• Co-immunoprecipitation (co-IP)

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Pierce™ Spin Columns - Screw Cap
Pierce™ Spin Cups - Paper Filter
Pierce™ Spin Cups - Cellulose Acetate Filter
Pierce™ Micro-Spin Columns

Pierce™ Micro-Spin Columns Thermo Scientific™

Thermo Scientific Pierce Micro-Spin Columns are convenient tools for manipulating small volumes of affinity supports (5 to 100 µL) for protein purification.

Features of Micro-Spin Columns:

Small volume: ideal for very small resin and sample volumes
Column volume: 400 µL
Resin volume: 5 to 100 µL
Filter type: Polyethylene filter, ~30 µm pore size
Cap: O-ring screw top caps; press-on bottom caps

Simply add the affinity resin and sample to one of the micro-spin columns, then use a microcentrifuge to efficiently wash away contaminants and elute your purified sample without losing any resin in the process. Micro-spin columns allow you to affinity purify more protein in less time.

Applications:
• Affinity purification or affinity chromatography
• Immunodepletion
• Immunoprecipitation (IP)
• Co-immunoprecipitation (co-IP)

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Pierce™ Spin Columns - Screw Cap
Pierce™ Spin Cups - Paper Filter
Pierce™ Spin Cups - Cellulose Acetate Filter
Pierce™ Spin Columns - Snap Cap

Pierce™ Centrifuge Columns, 0.8 mL Thermo Scientific™

Thermo Scientific Pierce Centrifuge Columns are convenient tools for handling 40 µL to 10 mL of an affinity support for purification.

Column details:

• Made from low protein binding polypropylene for compatibility with protein purification
• Total volume: 800 µL
• Resin volume: 40-400 µL
• Filter type, pore size: Polyethylene filter, ~30 µm pore size
• Receiver tube: Fits standard microcentrifuge tubes
• Caps: O-ring screw-top cap
• Twist-off bottom

Simply add the affinity resin to one of the polypropylene columns, remove the twist-off bottom and allow the resin to pack itself. Then add your sample and allow it to bind to the support and use a centrifuge to efficiently wash away any contaminants and elute your purified protein. Pierce Centrifuge Columns can be used in either spin or gravity-flow format. The spin format reduces sample processing time and makes column washing and elution more efficient. For gravity-flow procedures and when slow processing time is desired for binding steps with dilute samples, Column Extenders can be used to increase the column reservoir capacity. Use the any combination of processing techniques to get the best possible chromatography results.

Applications:

• Affinity purification, affinity chromatography
• Immunodepletion
• Ion exchange resins
• Gel filtration, desalting, buffer exchange

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Pierce™ Centrifuge Columns, 2 mL
Pierce™ Centrifuge Columns, 5 mL
Pierce™ Centrifuge Columns, 10 mL
Column Extenders for Pierce™ Centrifuge Columns

Pierce™ Centrifuge Columns, 2 mL Thermo Scientific™

Thermo Scientific Pierce Centrifuge Columns are convenient tools for handling 40 µL to 10 mL of an affinity support for purification.

Column details:

• Made from low protein binding polypropylene for compatibility with protein purification
• Total volume: 5 mL (2 mL resin bed, 3 mL reservoir)
• Resin volume: 2 mL
• Filter type, pore size: Polyethylene filter, ~30 µm pore size
• Receiver tube: Fits standard 15 mL conical centrifuge tubes
• Caps: Screw-top cap
• Twist-off bottom and press-on cap to reseal

Simply add the affinity resin to one of the polypropylene columns, remove the twist-off bottom and allow the resin to pack itself. Then add your sample and allow it to bind to the support and use a centrifuge to efficiently wash away any contaminants and elute your purified protein. Pierce Centrifuge Columns can be used in either spin or gravity-flow format. The spin format reduces sample processing time and makes column washing and elution more efficient. For gravity-flow procedures and when slow processing time is desired for binding steps with dilute samples, Column Extenders can be used to increase the column reservoir capacity. Use the any combination of processing techniques to get the best possible chromatography results.

Applications:

• Affinity purification, affinity chromatography
• Immunodepletion
• Ion exchange resins
• Gel filtration, desalting, buffer exchange

Related Products
Pierce™ Centrifuge Columns, 0.8 mL
Pierce™ Centrifuge Columns, 5 mL
Pierce™ Centrifuge Columns, 10 mL
Column Extenders for Pierce™ Centrifuge Columns

Pierce™ Spin Columns - Screw Cap Thermo Scientific™

Thermo Scientific Pierce Screw Cap Spin Columns are convenient tools for manipulating small volumes of affinity supports (20 to 400 µL) for protein purification.

Features of Screw Cap Spin Columns:

Adaptable: included Luer-Lok adaptors allow these columns to be used for syringe-based purifications
Column volume: 900µL
Resin volume: 20 to 400µL
Filter type: polyethylene filter, ~10µm pore size
Cap: O-ring screw-top caps
Frit options: included small & large frit options for different sample sizes

Simply add the affinity resin and sample to one of the spin columns, then use a microcentrifuge to efficiently wash away contaminants and elute your purified sample without losing any resin in the process. Spin columns allow you to affinity purify more protein in less time.

Applications:
• Affinity purification or affinity chromatography
• Immunodepletion
• Immunoprecipitation (IP)
• Co-immunoprecipitation (co-IP)

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Pierce™ Spin Cups - Paper Filter
Pierce™ Spin Cups - Cellulose Acetate Filter
Pierce™ Spin Columns - Snap Cap
Pierce™ Micro-Spin Columns

Pierce™ Centrifuge Columns, 5 mL Thermo Scientific™

Thermo Scientific Pierce Centrifuge Columns are convenient tools for handling 40 µL to 10 mL of an affinity support for purification.

Column details:

• Made from low protein binding polypropylene for compatibility with protein purification
• Total volume: 8 mL (5 mL resin bed, 3 mL reservoir)
• Resin volume: 5 mL
• Filter type, pore size: Polyethylene filter, ~30 µm pore size
• Receiver tube: Fits standard 15 mL conical centrifuge tubes
• Caps: Screw-top cap
• Twist-off bottom and press-on cap to reseal

Simply add the affinity resin to one of the polypropylene columns, remove the twist-off bottom and allow the resin to pack itself. Then add your sample and allow it to bind to the support and use a centrifuge to efficiently wash away any contaminants and elute your purified protein. Pierce Centrifuge Columns can be used in either spin or gravity-flow format. The spin format reduces sample processing time and makes column washing and elution more efficient. For gravity-flow procedures and when slow processing time is desired for binding steps with dilute samples, Column Extenders can be used to increase the column reservoir capacity. Use the any combination of processing techniques to get the best possible chromatography results.

Applications:

• Affinity purification, affinity chromatography
• Immunodepletion
• Ion exchange resins
• Gel filtration, desalting, buffer exchange

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Pierce™ Centrifuge Columns, 0.8 mL
Pierce™ Centrifuge Columns, 2 mL
Pierce™ Centrifuge Columns, 10 mL
Column Extenders for Pierce™ Centrifuge Columns

Column Extenders for Pierce™ Centrifuge Columns Thermo Scientific™

Thermo Scientific Pierce Centrifuge Column Extenders are made of polypropylene, and are designed for use in gravity-flow procedures. They fit 2 mL, 5 mL and 10 mL Pierce Centrifuge Columns.

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Pierce™ Centrifuge Columns, 0.8 mL
Pierce™ Centrifuge Columns, 2 mL
Pierce™ Centrifuge Columns, 5 mL
Pierce™ Centrifuge Columns, 10 mL

Pierce™ Disposable Columns, 5 mL Thermo Scientific™

Thermo Scientific Pierce Disposable Plastic Columns are empty columns with matching frits and caps for use in a variety of resin-based affinity or desalting techniques.

These bulk packages of empty columns are economical and convenient devices for packing affinity purification supports and desalting resins for gravity-flow procedures. Three columns sizes are offered, suitable for procedures requiring 0.5 to 10 mL resin-bed volumes. The plastic columns are supplied with matching pairs of polyethylene discs and polypropylene caps (top and bottom). The columns are economically-priced for single use and sufficiently durable to prepare and store columns for multiple uses.

Features of these disposable plastic columns:

• For benchtop gravity-flow affinity purification and size exclusion chromatography
• Supplied complete with porous polyethylene discs, stoppers and end caps
• Columns can be pre-packed and stored until needed
• Compatible with most types of aqueous buffer eluents commonly used in chromatography
• Plastic funnels can be attached to expand the buffer reservoir for increased flow rates

Applications:
• Affinity purification/Affinity chromatography
• Immunodepletion
• Size-exclusion chromatography

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Pierce™ Disposable Column Trial Pack
Pierce™ Disposable Columns, 2 mL
Pierce™ Disposable Columns, 10 mL
Column Funnels for Pierce™ Disposable Columns

Pierce™ Centrifuge Columns, 10 mL Thermo Scientific™

Thermo Scientific Pierce Centrifuge Columns are convenient tools for handling 40 µL to 10 mL of an affinity support for purification.

Column details:

• Made from low protein binding polypropylene for compatibility with protein purification
• Total volume: 22 mL (10 mL resin bed, 12 mL reservoir)
• Resin volume: 10 mL
• Filter type, pore size: Polyethylene filter, ~30 µm pore size
• Receiver tube: Fits standard 50 mL conical centrifuge tubes
• Caps: Screw-top cap
• Twist-off bottom and press-on cap to reseal

Simply add the affinity resin to one of the polypropylene columns, remove the twist-off bottom and allow the resin to pack itself. Then add your sample and allow it to bind to the support and use a centrifuge to efficiently wash away any contaminants and elute your purified protein. Pierce Centrifuge Columns can be used in either spin or gravity-flow format. The spin format reduces sample processing time and makes column washing and elution more efficient. For gravity-flow procedures and when slow processing time is desired for binding steps with dilute samples, Column Extenders can be used to increase the column reservoir capacity. Use the any combination of processing techniques to get the best possible chromatography results.

Applications:

• Affinity purification, affinity chromatography
• Immunodepletion
• Ion exchange resins
• Gel filtration, desalting, buffer exchange

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Pierce™ Centrifuge Columns, 0.8 mL
Pierce™ Centrifuge Columns, 2 mL
Pierce™ Centrifuge Columns, 5 mL
Column Extenders for Pierce™ Centrifuge Columns

Zeba™ Dye and Biotin Removal Spin Columns, 10 mL Thermo Scientific™

The Thermo Scientific Zeba Dye and Biotin Removal columns enable fast and efficient removal of non-reacted fluorescent dyes, biotin, reducing agents, and crosslinkers from protein samples. Removing free-dye after a labeling reaction is often difficult and time-consuming but is essential for accurate determination of dye-to-protein ratios. The Zeba Dye and Biotin Removal resin is highly specialized to produce exceptional protein recoveries while effectively removing non-conjugated fluorescent dyes, biotinylation reagents, reducing agents, and crosslinkers. Using the appropriate amount of sample and buffer conditions, almost any fluorescent dye can be removed with these columns. Even dilute protein samples (25 µg/mL) can be successfully processed to remove dyes, biotin, reducing agents, and crosslinkers and to efficiently recover proteins (>7 kDa). A single 10-mL Dye and Biotin Removal Spin Column can process a 2 to 4 mL of sample. This product is not recommended for desalting or buffer exchange.

Features of Zeba Dye and Biotin Removal columns:
Versatile—removes unreacted fluorescent dyes, biotinylation reagents, crosslinkers, and reducing agents from protein solutions
High recovery—low-binding resin maximizes protein recovery
Easy-to-use—no cumbersome column preparation or equilibration
Fast—small-molecule removal and protein recovery in less than 15 mins
Flexible—available in spin columns and filter spin plates for a range of needs

Available formats:
• Spin columns: 0.5, 2, 5, and 10 mL
• 96-well filter plates: pre-dispensed; compatible with centrifugation for manual or automated purification; enable fast, consistent well-to-well and plate-to-plate reproducibility for small-scale, high-throughput clean-ups

Applications:
• Ideal for post-reaction clean-up of samples
• To effectively remove non-conjugated dye, biotin, reducing agents, and crosslinkers with exceptional protein recovery

Zeba™ Dye and Biotin Removal Spin Columns, 5 mL Thermo Scientific™

The Thermo Scientific Zeba Dye and Biotin Removal columns enable fast and efficient removal of non-reacted fluorescent dyes, biotin, reducing agents, and crosslinkers from protein samples. Removing free-dye after a labeling reaction is often difficult and time-consuming but is essential for accurate determination of dye-to-protein ratios. The Zeba Dye and Biotin Removal resin is highly specialized to produce exceptional protein recoveries while effectively removing non-conjugated fluorescent dyes, biotinylation reagents, reducing agents, and crosslinkers. Using the appropriate amount of sample and buffer conditions, almost any fluorescent dye can be removed with these columns. Even dilute protein samples (25 µg/mL) can be successfully processed to remove dyes, biotin, reducing agents, and crosslinkers and to efficiently recover proteins (>7 kDa). A single 5-mL Dye and Biotin Removal Spin Column can process a 1 to 2 mL of sample. This product is not recommended for desalting or buffer exchange.

Features of Zeba Dye and Biotin Removal columns:
Versatile—removes unreacted fluorescent dyes, biotinylation reagents, crosslinkers, and reducing agents from protein solutions
High recovery—low-binding resin maximizes protein recovery
Easy-to-use—no cumbersome column preparation or equilibration
Fast—small-molecule removal and protein recovery in less than 15 mins
Flexible—available in spin columns and filter spin plates for a range of needs

Available formats:
• Spin columns: 0.5, 2, 5, and 10 mL
• 96-well filter plates: pre-dispensed; compatible with centrifugation for manual or automated purification; enable fast, consistent well-to-well and plate-to-plate reproducibility for small-scale, high-throughput clean-ups

Applications:
• Ideal for post-reaction clean-up of samples
• To effectively remove non-conjugated dye, biotin, reducing agents, and crosslinkers with exceptional protein recovery

Column Funnels for Pierce™ Disposable Columns Thermo Scientific™

These disposable column funnels are designed for use with Thermo Scientific Disposable Columns.

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Zeba™ Dye and Biotin Removal Filter Plates, 96 well Thermo Scientific™

The Thermo Scientific Zeba Dye and Biotin Removal columns enable fast and efficient removal of non-reacted fluorescent dyes, biotin, reducing agents, and crosslinkers from protein samples. Removing free-dye after a labeling reaction is often difficult and time-consuming but is essential for accurate determination of dye-to-protein ratios. The Zeba Dye and Biotin Removal resin is highly specialized to produce exceptional protein recoveries while effectively removing non-conjugated fluorescent dyes, biotinylation reagents, reducing agents, and crosslinkers. Using the appropriate amount of sample and buffer conditions, almost any fluorescent dye can be removed with these columns. Even dilute protein samples (25 µg/mL) can be successfully processed to remove dyes, biotin, reducing agents, and crosslinkers and to efficiently recover proteins (>7 kDa). Each well of the Zeba Dye and Biotin Removal 96-well filter plate can process a 40 to 100 µL of sample. This product is not recommended for desalting or buffer exchange.

Features of Zeba Dye and Biotin Removal columns:
Versatile—removes unreacted fluorescent dyes, biotinylation reagents, crosslinkers, and reducing agents from protein solutions
High recovery—low-binding resin maximizes protein recovery
Easy-to-use—no cumbersome column preparation or equilibration
Fast—small-molecule removal and protein recovery in less than 15 mins
Flexible—available in spin columns and filter spin plates for a range of needs

Available formats:
• Spin columns: 0.5, 2, 5, and 10 mL
• 96-well filter plates: pre-dispensed; compatible with centrifugation for manual or automated purification; enable fast, consistent well-to-well and plate-to-plate reproducibility for small-scale, high-throughput clean-ups

Applications:
• Ideal for post-reaction clean-up of samples
• To effectively remove non-conjugated dye, biotin, reducing agents, and crosslinkers with exceptional protein recovery

Zeba™ Dye and Biotin Removal Spin Columns, 0.5 mL Thermo Scientific™

The Thermo Scientific Zeba Dye and Biotin Removal columns enable fast and efficient removal of non-reacted fluorescent dyes, biotin, reducing agents, and crosslinkers from protein samples. Removing free-dye after a labeling reaction is often difficult and time-consuming but is essential for accurate determination of dye-to-protein ratios. The Zeba Dye and Biotin Removal resin is highly specialized to produce exceptional protein recoveries while effectively removing non-conjugated fluorescent dyes, biotinylation reagents, reducing agents, and crosslinkers. Using the appropriate amount of sample and buffer conditions, almost any fluorescent dye can be removed with these columns. Even dilute protein samples (25 µg/mL) can be successfully processed to remove dyes, biotin, reducing agents, and crosslinkers and to efficiently recover proteins (>7 kDa). A single 0.5-mL Dye and Biotin Removal Spin Column can process a 50 to 120 µL of sample. This product is not recommended for desalting or buffer exchange.

Features of Zeba Dye and Biotin Removal columns:
Versatile—removes unreacted fluorescent dyes, biotinylation reagents, crosslinkers, and reducing agents from protein solutions
High recovery—low-binding resin maximizes protein recovery
Easy-to-use—no cumbersome column preparation or equilibration
Fast—small-molecule removal and protein recovery in less than 15 mins
Flexible—available in spin columns and filter spin plates for a range of needs

Available formats:
• Spin columns: 0.5, 2, 5, and 10 mL
• 96-well filter plates: pre-dispensed; compatible with centrifugation for manual or automated purification; enable fast, consistent well-to-well and plate-to-plate reproducibility for small-scale, high-throughput clean-ups

Applications:
• Ideal for post-reaction clean-up of samples
• To effectively remove non-conjugated dye, biotin, reducing agents, and crosslinkers with exceptional protein recovery

Pierce™ Microcentrifuge Tubes, 2.0 mL Thermo Scientific™

Thermo Scientific Pierce 2 mL Capacity Microcentrifuge Tubes are convenient tools for manipulating small volumes of affinity supports for protein purification.

Features of 2 mL Capacity Microcentrifuge Tubes:

• 1.5 mL capacity
• Polypropylene formulation
• Attached snap caps

Simply add the affinity resin and sample to one of the microcentrifuge tubes, then use a microcentrifuge to efficiently wash away contaminants and elute your purified sample without losing any resin in the process. Microcentrifuge tubes allow you to affinity purify more protein in less time.

Applications:
• Affinity purification or affinity chromatography
• Immunodepletion
• Immunoprecipitation (IP)
• Co-immunoprecipitation (co-IP)

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Pierce™ Microcentrifuge Tubes, 1.5 mL
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