DNA polymerases are enzymes that play a crucial role in DNA replication, as they catalyze the formation of new DNA strands using existing ones as templates. Two key polymerases involved in this process are DNA polymerase 1 (Pol 1) and DNA polymerase 3 (Pol 3). Pol 1 is primarily involved in removing RNA primers and filling in the gaps with DNA nucleotides during DNA replication, while Pol 3 is the main polymerase responsible for synthesizing new DNA strands.
Our DNA Polymerases category features a range of products designed for use in various industries, including pharmaceuticals, biotechnology, and research institutions. These products are essential for ensuring accurate and precise amplification of DNA samples in research, diagnostics, and forensic applications. The category includes a variety of DNA polymerases such as Taq polymerase, Phusion polymerase, and Platinum polymerase, each with specific advantages depending on the application. Additionally, the category features PCR master mixes, PCR enzymes, and other PCR reagents for efficient DNA amplification.
DNA Polymerase I is a DNA polymerase with 5´→3´ and 3´→5´ exodeoxyribonuclease activities. DNA Polymerase I also incorporates biotinylated nucleotides. • Applications—DNase I-dependent nick translation, second-strand synthesis in cDNA cloning, fill-in of 5´ overhangs • Source—purified from E.
Platinum™ Tfi DNA Polymerase combines Platinum™ automated hot-start technology with a non-Taq, surfactant-free PCR enzyme derived from Thermus filiformis. PCR performance is comparable to Platinum™ Taq DNA polymerase in yield, specificity, fidelity, and robustness for targets less than 1 kb in...
Thermo Scientific DNA Polymerase I, a template-dependent DNA polymerase, catalyzes 5'→3' synthesis of DNA. The enzyme also exhibits 3'→5' exonuclease (proofreading) activity, 5'→3' exonuclease activity, and ribonuclease H activity. Highlights • Incorporates modified nucleotides (e.g.
Thermo Scientific T4 DNA Polymerase is a template-dependent DNA polymerase that catalyzes 5'-3' synthesis from primed single-stranded DNA. The enzyme has a 3'-5' exonuclease activity, but lacks 5'-3' exonuclease activity.
Thermo Scientific T7 DNA Polymerase, a template dependent DNA polymerase, catalyzes DNA synthesis in the 5'→3' direction. It is a highly processive DNA polymerase allowing continuous synthesis of long stretches of DNA.
DNA Polymerase I/DNase I is an optimized mixture of both enzymes for efficient nick translation of DNA. Application: Labeling DNA with either radiolabeled or biotinylated nucleotides. Source: DNase I is purified from bovine pancreas; DNA Polymerase I from E. coli λ lysogen NM 964.
Thermo Scientific dsDNase is an engineered shrimp DNase designed for rapid and safe removal of contaminating genomic DNA from RNA samples. It is an endonuclease that cleaves phosphodiester bonds in DNA to yield oligonucleotides with 5'-phosphate and 3'-hydroxyl termini.
T4 DNA Polymerase is a DNA polymerase that has a 3´-exodeoxyribonuclease activity, but lacks 5´a3´ exodeoxyribonuclease activity. A T4 DNA Polymerase Technical Bulletin is available. Applications: Labeling double-stranded linear DNA by replacement synthesis (1).
Catalyzes polymeration of deoxyribonucleotides into DNA strands. Suitable for PCR and automated sequencing reactions This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand.
DNA Polymerase I, Large (Klenow) Fragment is a DNA polymerase enzyme that lacks the 5' to 3' exonuclease activity of intact DNA Polymerase I, but does exhibit the 5' to 3' DNA polymerase and 3' to 5' exonuclease activities. Applications: Fill-in of 5´ overhangs (1).