Restriction Enzymes

Restriction Enzymes

Enzymes that locate and bind to specific sequences (restriction sites) on DNA molecules and cleave the DNA into fragments at or near those sites. Products include a variety of restriction enzymes and buffers in various quantities and concentrations.
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1-15 of 546 Products

FastDigest BamHI Thermo Scientific™

5' G ↓ G A T C C 3' 3' C C T A G ↑ G 5' Thermo Scientific FastDigest BamHI restriction enzyme recognizes G^GATCC site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer. Thermo Scientific FastDigest BamHI is one of an advanced line of fast restriction enzymes that are all 100%...

BamHI (10 U/μL) Thermo Scientific™

5' G ↓ G A T C C 3' 3' C C T A G ↑ G 5' Thermo Scientific BamHI restriction enzyme recognizes G^GATCC sites and cuts best at 37°C in its own unique buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this...

BcuI (SpeI) (10 U/μL) Thermo Scientific™

5' A ↓ C T A G T 3' 3' T G A T C ↑ A 5' Thermo Scientific BcuI (SpeI) restriction enzyme recognizes A^CTAGT sites and cuts best at 37°C in Tango buffer (isoschizomers: AhlI, SpeI). See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and...

FastDigest AanI Thermo Scientific™

5' T T A ↓ T A A 3' 3' A A T ↑ A T T 5' Thermo Scientific FastDigest AanI restriction enzyme recognizes TTA^TAA sites and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer. Isoschizomers: PsiI.

GTP Solution (100 mM) Thermo Scientific™

Thermo Scientific GTP (guanosine 5'-triphosphate) is supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH. It is more than 99% pure and highly stable. GTP may be used in a wide variety of different molecular biology applications.

Bpu1102I (BlpI) (10 U/μL) Thermo Scientific™

5' G C ↓ T N A G C 3' 3' C G A N T ↑ C G 5' Thermo Scientific Bpu1102I (BlpI) restriction enzyme recognizes GC^TNAGC sites and cuts best at 37°C in Tango buffer (Isoschizomers: BlpI, Bsp1720I, CelII). See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for...

AarI (2 U/μL) Thermo Scientific™

5' C A C C T G C N4 ↓ 3' 3' G T G G A C G N8 ↑ 5' Thermo Scientific AarI restriction enzyme recognizes CACCTGC(4/8)^ sites and cuts best at 37°C in its own unique (+oligo) buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and...

AdeI (DraIII) (10 U/μL) Thermo Scientific™

5' C A C N N N ↓ G T G 3' 3' G T G ↑ N N N C A C 5' Thermo Scientific AdeI (DraIII) restriction enzyme recognizes CACNNN^GTG sites and cuts best at 37°C in G buffer (isoschizomers: DraIII). See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double...

Bsh1236I (BstUI) (10 U/μL) Thermo Scientific™

5' C G ↓ C G 3' 3' G C ↑ G C 5' Thermo Scientific Bsh1236I (BstUI) restriction enzyme recognizes CG^CG sites and cuts best at 37°C in R buffer (isoschizomers: AccII, BspFNI, BstFNI, BstUI, MvnI). See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double...

FastDigest AdeI Thermo Scientific™

5' C A C N N N ↓ G T G 3' 3' G T G ↑ N N N C A C 5' Thermo Scientific FastDigest AdeI restriction enzyme recognizes CACNNN^GTG site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer. Isoschizomers: DraIII, BstIZ316I.

AanI (PsiI) (10 U/μL) Thermo Scientific™

5' T T A ↓ T A A 3' 3' A A T ↑ A T T 5' Thermo Scientific AanI (PsiI) restriction enzyme recognizes TTA^TAA sites and cuts best at 37°C in Tango buffer (isoschizomers: PsiI). See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat...

FastDigest AatII Thermo Scientific™

5' G A C G T ↓ C 3' 3' C ↑ T G C A G 5' Thermo Scientific FastDigest AatII restriction enzyme recognizes GACGT^C sites and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer. Isoschizomers: ZraI.

TBE Buffer (Tris-borate-EDTA) (10X) Thermo Scientific™

Thermo Scientific 10X TBE Buffer (Tris-borate-EDTA) is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel.

AarI-AjiI-Bpu10I-ScaI-PasI Buffer (10X) Thermo Scientific™

Thermo Scientific 10X Buffer AarI, AjiI, Bpu10I, ScaI, PasI is the optimal buffer recommended for use with AarI, AjiI, Bpu10I, ScaI, and PasI restriction enzymes and is premixed with BSA for enhanced stability.

Reaction Buffer with MgCl2 for DNase I (10X) Thermo Scientific™

Thermo Scientific 10X Reaction Buffer with MgCl2 is used with RNase-free DNase I, an endonuclease that digests single- and double-stranded DNA.
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