Restriction Enzymes

Restriction Enzymes

Enzymes that locate and bind to specific sequences (restriction sites) on DNA molecules and cleave the DNA into fragments at or near those sites. Products include a variety of restriction enzymes and buffers in various quantities and concentrations.
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Sensitive to Heat Inactivation

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FastDigest XhoI Thermo Scientific™

5' C ↓ T C G A G 3' 3' G A G C T ↑ C 5' Thermo Scientific FastDigest XhoI restriction enzyme recognizes C^TCGAG site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer. Isoschizomers: PaeR7I, Sfr274I, SlaI, StrI, TliI.

FastDigest MboI Thermo Scientific™

5' ↓ G A T C 3' 3' C T A G ↑ 5' Thermo Scientific FastDigest MboI restriction enzyme recognizes ^GATC site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer. Isoschizomers: BfuCI, BssMI, BstKTI, BstMBI, DpnII, Kzo9I, NdeII, Sau3AIm, Bsp143I.

FastDigest RsaI Thermo Scientific™

5' G T ↓ A C 3' 3' C A ↑ T G 5' Thermo Scientific FastDigest RsaI restriction enzyme recognizes GT^AC site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer. Isoschizomers: AfaI, CviQI, RsaNI.

FastDigest SmiI Thermo Scientific™

5' A T T T ↓ A A A T 3' 3' T A A A ↑ T T T A 5' Thermo Scientific FastDigest SmiI restriction enzyme recognizes ATTT^AAAT site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer. Isoschizomers: SwaI, BstRZ246I, BstSWI, MspSWI.

FastDigest TatI Thermo Scientific™

5' W ↓ G T A C W 3' 3' W C A T G ↑ W 5' Thermo Scientific FastDigest TatI restriction enzyme recognizes W^GTACW site and cuts best at 65°C in 5–15 minutes using universal FastDigest Buffer. Thermo Scientific FastDigest TatI is one of an advanced line of fast restriction enzymes that are all 100%...

FastDigest KspAI Thermo Scientific™

5' G T T ↓ A A C 3' 3' C A A ↑ T T G 5' Thermo Scientific FastDigest KspAI restriction enzyme recognizes GTT^AAC site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer. Isoschizomers: HpaI, BsaKI, BseII, BstEZ359I, BstHPI, MwhI, SsrI, Uba1408II.

FastDigest XagI Thermo Scientific™

5' C C T N N ↓ N N N A G G 3' 3' G G A N N N ↑ N N T C C 5' Thermo Scientific FastDigest XagI restriction enzyme recognizes CCTNN^NNNAGG site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer. Isoschizomers: EcoNI, BstENI.

FastDigest BoxI Thermo Scientific™

5' G A C N N ↓ N N G T C 3' 3' C T G N N ↑ N N C A G 5' Thermo Scientific FastDigest BoxI restriction enzyme recognizes GACNN^NNGTC site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer. Isoschizomers: PshAI, BstPAI.

FastDigest OliI Thermo Scientific™

5' C A C N N ↓ N N G T G 3' 3' G T G N N ↑ N N C A C 5' Thermo Scientific FastDigest OliI restriction enzyme recognizes CACNN^NNGTG site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer. Isoschizomers: AleI.

FastDigest MauBI Thermo Scientific™

5' C G ↓ C G C G C G 3' 3' G C G C G C ↑ G C 5' Thermo Scientific FastDigest MauBI restriction enzyme recognizes CG^CGCGCG site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer. Thermo Scientific FastDigest MauBI is one of an advanced line of fast restriction enzymes that are...

FastDigest RruI Thermo Scientific™

5' T C G ↓ C G A 3' 3' A G C ↑ G C T 5' Thermo Scientific FastDigest RruI restriction enzyme recognizes TCG^CGA site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer. Isoschizomers: NruI, BtuMI.

FastDigest AdeI Thermo Scientific™

5' C A C N N N ↓ G T G 3' 3' G T G ↑ N N N C A C 5' Thermo Scientific FastDigest AdeI restriction enzyme recognizes CACNNN^GTG site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer. Isoschizomers: DraIII, BstIZ316I.

FastDigest PagI Thermo Scientific™

5' T ↓ C A T G A 3' 3' A G T A C ↑ T 5' Thermo Scientific FastDigest PagI restriction enzyme recognizes T^CATGA site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer. Isoschizomers: BspHI, CciI, RcaI.

FastDigest FspAI Thermo Scientific™

5' R T G C ↓ G C A Y 3' 3' Y A C G ↑ C G T R 5' Thermo Scientific FastDigest FspAI restriction enzyme recognizes RTGC^GCAY site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer. Thermo Scientific FastDigest FspAI is one of an advanced line of fast restriction enzymes that are...

FastDigest BspOI Thermo Scientific™

5' G C T A G ↓ C 3' 3' C ↑ G A T C G 5' Thermo Scientific FastDigest BspOI restriction enzyme recognizes GCTAG^C site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer. Isoschizomers: BmtI, AsuNHI.
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