Substrates

CDP-Star™ Substrate (12.5 mM Concentrate) Invitrogen™

This concentrated CDP-Star™ Substrate is a chemiluminescent alkaline phosphatase substrate for protein or nucleic acid blotting on nitrocellulose membranes. This substrate can also be used in solution-based assays.

Fluorescein Di-β-D-Galactopyranoside (FDG) Invitrogen™

Fluorescein di-β-D-galactopyranoside (FDG) is one of the most sensitive substrates for galactosidases. Nonfluorescent FDG is sequentially hydrolyzed by β-galactosidase, first to fluorescein monogalactoside (FMG) and then to highly fluorescent fluorescein.

DQ™ Green BSA - Special Packaging Invitrogen™

DQ Green BSA is a fluorogenic substrate for proteases. A strong fluorescence quenching effect is observed when proteins are heavily labeled with BODIPY dyes. Upon hydrolysis of the DQ Green BSA to single, dye-labeled peptides by proteases, this quenching is relieved, producing brightly fluorescent...

C₁₂FDG (5-Dodecanoylaminofluorescein Di-β-D-Galactopyranoside) Invitrogen™

The β-galactosidase substrate, C12FDG has been covalently modified to include a 12-carbon lipophilic moiety. Once inside the cell, the substrate is cleaved by β-galactosidase producing a fluorescent product that is well retained by the cells, probably by incorporation of the liphophilic tail within...

Novex™ AP Chemiluminescent Substrate (CDP-Star™) Invitrogen™

Novex® AP Chemiluminescent Substrate is a non-radioactive ready-to-use solution of CDP-Star for chemiluminescence-based immunodetection of alkaline phosphatase (AP) on western blot or dot blot membranes.

DQ™ Ovalbumin - Special Packaging Invitrogen™

DQ ovalbumin is a fluorogenic substrate for proteases. A strong fluorescence quenching effect is observed when proteins are heavily labeled with BODIPY dyes. Upon hydrolysis of the DQ ovalbumin to single, dye-labeled peptides by proteases, this quenching is relieved, producing brightly fluorescent...

Amplex™ UltraRed Reagent Invitrogen™

Our Amplex UltraRed reagent improves upon the performance of our unique Amplex Red reagent, offering brighter fluorescence and enhanced sensitivity on a per-mole basis in peroxidase or peroxidase-coupled enzyme assays.

DiFMUP (6,8-Difluoro-4-Methylumbelliferyl Phosphate) Invitrogen™

DiFMUP is a fluorinated 4-methylumbelliferyl phosphate (MUP) derivative that has a lower pKa than that of MUP, making DiFMUP an excellent substrate for continuously assaying acid phosphatases at low pH. The reaction product of DiFMUP has excitation/emission maxima of ∼358/450 nm.

Nitro blue Tetrazolium Chloride (NBT) Invitrogen™

Nitro blue tetrazolium chloride (NBT) can be used to assess cell viability as a function of redox potential. Actively respiring cells convert the water-soluble NBT to a deep blue-colored precipitate.

NBT/BCIP Reagent Kit Invitrogen™

The NBT/BCIP Reagent Kit includes 1 g samples of both NBT and BCIP for the chromogenic detection of phosphatase.

DDAO galactoside (9H-(1,3-Dichloro-9,9-Dimethylacridin-2-One-7-yl) β-D-Galactopyranoside) Invitrogen™

The galactosidase substrate, DDAO galactoside yields a hydrolysis product that can be excited with the 633 nm laser (excitation/emission maxima ∼645/660). Although the substrate itself is fluorescent (excitation/emission maxima ∼460/610 nm), the difference between the substrate's and the hydrolysis...

PED-A1 (N-((6-(2,4-DNP)Amino)Hexanoyl)-1-(BODIPY™ FL C5)-2-Hexyl-Sn-Glycero-3-Phosphoethanolamine) (Phospholipase A1 Selective Substrate) Invitrogen™

Molecular Probes™ phospholipase substrates are dye labeled phospholipids used to monitor phospholipase A (PLA) activity in purified enzyme preparations, cell lysates, and living cells. Two structural variations of these substrates are available, which confer specificity for PLA1 and PLA2.

Sphingosine Kinase Substrate Thermo Scientific™

Sphingosine substrate specifically optimized for use with the Sphingosine kinases (SPHK1 & SPHK2). For use with the Adapta™ Universal Kinase Assay (PV5099).

Fluorescein-ERM (LRRKtide)

A fluorescein-labeled peptide substrate based upon ezrin/radixin/moesin (ERM) that is phosphorylated by LRRK2 and LRRK2 mutants. For use in combination with the LanthaScreen™ Tb-anti-ERM (pLRRKtide) Antibody (PV4899) to evalute inhibitors in medium to high throughput screening applications.

GFP-4E-BP1 Thermo Scientific™

4E-BP1 expressed as a fusion with GFP (Green Fluorescent Protein) to act as a physiological substrate for FRAP1 (mTor). For use in combination with the LanthaScreen™ Tb-anti-4E-BP1 [pThr46] Antibody (PV4757) to evaluate inhibitors in medium to high throughput screening applications.