Substrates

A variety of substrates in various quantities designed for biological and chemical analyses, the initiation of chemical reactions, culturing procedures, and use as a matrix for organisms to live in or on. Products are suited for specialty applications.
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CCF2-FA (Free Acid) is a fluorescence resonance energy transfer (FRET) substrate that consists of a cephalosporin core linking B7-hydroxycoumarin to fluorescein. This product is intended for use as a beta-lactamase substrate in a GeneBLAzer™ cell-based assay.
The EnzChek™ cellulase substrate was developed for simple and rapid quantitation of cellulase. The assay can be completed in 30 minutes or less. Simply mix the enzyme with the EnxChek™ cellulase substrate working solution, incubate, and read the results using excitation/emission maxima ∼339/452 nm.
Nitro blue tetrazolium chloride (NBT) can be used to assess cell viability as a function of redox potential. Actively respiring cells convert the water-soluble NBT to a deep blue-colored precipitate.
CELLstart™ is a defined substrate, containing only components of human origin (xeno-free).•Defined, xenofree substrate for stem cell culture •cGMP Manufactured to help ensure manufacturability and traceability CELLstart™ is a defined substrate, containing only components of human origin (xeno-free).
The galactosidase substrate, DDAO galactoside yields a hydrolysis product that can be excited with the 633 nm laser (excitation/emission maxima ∼645/660). Although the substrate itself is fluorescent (excitation/emission maxima ∼460/610 nm), the difference between the substrate's and the hydrolysis...
IPTG Thermo Scientific™
Thermo Scientific™ Pierce beta-Galactosidase Precipitating Substrates is a chromogenic substrate for beta-galactosidase that is used to detect expression of vectors, plasmids, or DNA fragments that contain the reporter gene.
The nonfluorescent EnzChek™ lipase substrate, in the presence of lipases, produces a green-fluorescent product (excitation/emission maxima ∼507/515) for accurate lipase detection in solution. Dissolve in high-quality anhydrous DMSO or chloroform to prepare a stock concentrations of up to 1 mM.
The fluorogenic DQ™ collagen can be used to directly monitor collagenase activity. DQ™ substrates are analogs of the natural substrate that have an excessive number of fluorescent dyes attached so that the fluorescence signal is almost non-existent.
The CMAC peptidase substrate, t-BOC-Leu-Met can be used to measure peptidase activity in solution or in live cells. The substrate has an excitation/emission maxima ∼330/403. After cleavage by peptidases, the product produces blue-fluorescence with excitation/emission maxima ∼351/430.
Vivid™ DBOMF Substrate Thermo Scientific™
Vivid™ Substrates are blocked dyes that yield a minimal fluorescence signal until oxidation occurs. Cytochrome P450 metabolism at either of two potential oxidation sites releases the highly fluorescent product.
Vivid™ BOMR Substrate Thermo Scientific™
Vivid™ Substrates are blocked dyes that yield a minimal fluorescence signal until oxidation occurs. Cytochrome P450 metabolism at either of two potential oxidation sites releases the highly fluorescent product.
Specifically optimized for use with the Class I PI3 kinases (PIK3CA, PIK3CB, PIK3CD, and PIK3CG), this substrate consists of lipid vesicles of a 1:19 molar ratio of phosphoinositol-4,5-bisphosphate (PIP2) with phosphotidylserine (PS). For use with the Adapta™ Universal Kinase Assay (PV5099).
EIF2A has been expressed as a fusion with GFP (Green Fluorescent Protein) to act as a physiological substrate for the EIF2AK family of kinases including PERK (EIF2AK3) and PKR (EIF2AK2). For use in combination with the LanthaScreen™ Tb-anti-eIF2alpha [pSer52] Antibody (PV4810) to evaluate inhibitors...
The ß-glucosidase substrate, 5-(pentafluorobenzoylamino)fluorescein di-ß-D-glucopyranoside (PFB-FDGlu) yields the green-fluorescent PFB-F dye (excitation/emission maxima ∼492/516 nm), which appears to localize to endosomal and lysosomal compartments when loaded into cells by pinocytosis.
The fluorogenic β-galactosidase substrate resorufin β-D-galactopyranoside yields the hydrolysis product, resorufin, with absorption/emission maxima at 571/585 nm.
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Bluo-gal and X-gal are commonly used substrates for measuring enzymatic activity of beta-galactosidase in bacteria. Life Technologies offers these enzyme substrates in several sizes for added convenience.
Enzyme-linked immunoassays (ELISAs) are used to quantitate specific proteins in solution. The detection enzyme can be introduced into the assay in a number of ways ( Figure 1 ). Horseradish peroxidase (HRP) and alkaline phosphatase (AP) are the most commonly used enzyme labels, mainly due to the...
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Which cell lines has CELLstart substrate been tested with?
Can I use the CELLstart substrate if there is gelling in the vial?