Substrates

Substrates

A variety of substrates in various quantities designed for biological and chemical analyses, the initiation of chemical reactions, culturing procedures, and use as a matrix for organisms to live in or on. Products are suited for specialty applications.
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Substrate

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CSPD™ Substrate (25 mM Concentrate) Invitrogen™

This concentrated CSPD™ substrate is a chemiluminescent alkaline phosphatase substrate for protein or nucleic acid blotting on nitrocellulose membranes. This substrate can also be used in solution-based assays.

Fluorescein Di-β-D-Galactopyranoside (FDG) Invitrogen™

Fluorescein di-β-D-galactopyranoside (FDG) is one of the most sensitive substrates for galactosidases. Nonfluorescent FDG is sequentially hydrolyzed by β-galactosidase, first to fluorescein monogalactoside (FMG) and then to highly fluorescent fluorescein.

Vivid™ DBOMF Substrate Thermo Scientific™

Vivid® Substrates are blocked dyes that yield a minimal fluorescence signal until oxidation occurs. Cytochrome P450 metabolism at either of two potential oxidation sites releases the highly fluorescent product.

Vivid™ EOMCC Substrate Thermo Scientific™

Vivid® Substrates are blocked dyes that yield a minimal fluorescence signal until oxidation occurs. Cytochrome P450 metabolism at either of two potential oxidation sites releases the highly fluorescent product.

NBT/BCIP Reagent Kit Invitrogen™

The NBT/BCIP Reagent Kit includes 1 g samples of both NBT and BCIP for the chromogenic detection of phosphatase.

Nitro blue Tetrazolium Chloride (NBT) Invitrogen™

Nitro blue tetrazolium chloride (NBT) can be used to assess cell viability as a function of redox potential. Actively respiring cells convert the water-soluble NBT to a deep blue-colored precipitate.

DQ™ Collagen, type I From Bovine Skin, Fluorescein Conjugate Invitrogen™

The fluorogenic DQ™ collagen can be used to directly monitor collagenase activity. DQ™ substrates are analogs of the natural substrate that have an excessive number of fluorescent dyes attached so that the fluorescence signal is almost non-existent.

X-GlcU, CHA (5-Bromo-4-Chloro-3-Indolyl β-D-Glucuronide, Cyclohexylammonium Salt) Invitrogen™

X-GlcU is a widely used chromogenic substrate for β-glucuronidase (GUS). It yields a dark blue precipitate at the site of enzymatic activity. X-GlcU is routinely used to detect GUS expression in transformed plant cells and tissues. X-GlcU can also be used to detect E.

CMAC, t-BOC-Leu-Met (7-Amino-4-Chloromethylcoumarin, t-BOC-L-Leucyl-L-Methionine amide) Invitrogen™

The CMAC peptidase substrate, t-BOC-Leu-Met can be used to measure peptidase activity in solution or in live cells. The substrate has an excitation/emission maxima ∼330/403. After cleavage by peptidases, the product produces blue-fluorescence with excitation/emission maxima ∼351/430.

Elastase Substrate V Thermo Scientific™

This Thermo Scientific brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific.

Red/Green BODIPY™ PC-A2 (1-O-(6-BODIPY™ 558/568-Aminohexyl)-2-BODIPY™ FL C5-Sn-Glycero-3-Phosphocholine) (Ratiometric Phospholipase A2 Substrate) Invitrogen™

Molecular Probes® phospholipase substrates are dye labeled phospholipids used to monitor phospholipase A (PLA) activity in purified enzyme preparations, cell lysates, and living cells. Two structural variations of these substrates are available, which confer specificity for PLA1 and PLA2.

Amplex™ Red Reagent Invitrogen™

The Amplex Red reagent, a highly sensitive and stable probe for H2O2, is our best fluorogenic substrate for peroxidase. In the presence of horseradish peroxidase (HRP), the Amplex Red reagent reacts in a 1:1 stoichiometry with H2O2 to produce highly fluorescent resorufin.

PED-A1 (N-((6-(2,4-DNP)Amino)Hexanoyl)-1-(BODIPY™ FL C5)-2-Hexyl-Sn-Glycero-3-Phosphoethanolamine) (Phospholipase A1 Selective Substrate) Invitrogen™

Molecular Probes® phospholipase substrates are dye labeled phospholipids used to monitor phospholipase A (PLA) activity in purified enzyme preparations, cell lysates, and living cells. Two structural variations of these substrates are available, which confer specificity for PLA1 and PLA2.

beta-Galactosidase Assay Reagent Thermo Scientific™

The Thermo Scientific Mammalian β-Galactosidase Assay Reagent is a component of the Mammalian β-Galactosidase Assay Kit, which provides a simple colorimetric method for lysing cultured mammalian cells and measuring beta-Gal activity.

Fluorescein-Crosstide Substrate

A general substrate for Ser/Thr kinases, the Crosstide peptide substrate is based upon GSK-3alpha/beta. For use in combination with the LanthaScreen® Tb-pCrosstide Antibody (PV3564) to evaluate inhibitors in medium to high throughput screening applications.
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