Shop All Antibody-Binding Proteins and Conjugates

ProLong™ Live Antifade Reagent, for live cell imaging Invitrogen™

ProLong® Live Antifade Reagent helps prevent the loss of fluorescent signal due to photobleaching during live cell imaging. When dyes are illuminated, they can degrade (usually permanently), reducing the sample’s fluorescent intensity and leading to the creation of free radical singlet oxygen, an element that can further degrade neighboring dye molecules. ProLong® Live Antifade Reagent metabolizes this element, increasing the signal intensity and duration while keeping the background signal low. ProLong® Live reagent has little-to-no effect on cellular viability, proliferation, or other cellular functions in experiments up to 48 hours.

Features of ProLong® Live Antifade Reagent include:
• Photobleach protection in live cell experiments, for fluorophores such as GFP, RFP, Hoechst 33342, MitoTracker®, LysoTracker®, and CellTracker®dyes
• Little-to-no effect on cellular viability, proliferation, or other cellular functions
• Easy to use, just add to the imaging solution or complete cell culture media or buffer, incubate, and image

ProLong® Live Antifade Reagent contains enzymes from the plasma membrane of naturally occurring E. coli. These enzymes metabolize the elements that can cause photobleaching. They work in the extracellular space and are not known to enter cells, so intracellular functions are minimally affected by ProLong® Live Antifade Reagent. As shown in figure below, cells grown in the presence of ProLong® Live Antifade Reagent display minimal effects on cellular viability or proliferation. ProLong® Live Antifade Reagent provides photobleach protection for most fluorophores frequently used in live cell fluorescent imaging, such as GFP, RFP, Hoechst 33342, MitoTracker®, LysoTracker® and CellTracker® dyes. ProLong® Live Antifade Reagent can be added to any cell culture media or buffer that is suitable for fluorescent imaging.

CaptureSelect™ Biotin Anti-FIX Conjugate Thermo Scientific™

CaptureSelect™ Biotin Anti-FIX Conjugate consists of a 13 kDa camelid antibody fragment (affinity ligand) with high affinity and selectivity for both recombinant and plasma derived human Factor IX (FIX). The affinity ligand is chemically conjugated to biotin via an appropriate spacer that retains the binding reactivity of the ligand when used in combination with streptavidin-based conjugates or streptavidin pre-coated surfaces.

The CaptureSelect™ Biotin Anti-FIX Conjugate allows you to:

Detect, quantitate, and characterize recombinant and plasma derived human FIX

Applications for CaptureSelect™ Biotin Anti-FIX Conjugate include ELISA, Western blot, Gyrolab™-based immunoassays, and label-free detection platforms such as those based on surface plasmon resonance (Biacore™ and IBIS-MX96 systems) and bio-layer interferometry (ForteBio™ Octet™ systems).

SiteClick™ Antibody Azido Modification Kit Invitrogen™

Create a label-ready site-specific azido-modified antibody without lengthy and inefficient genetic modification using the SiteClick Antibody Azido Modification Kit. SiteClick labeling uses enzymes to specifically attach an azido moiety to the heavy chains of an IgG antibody, ensuring that the antigen binding domains remain unaltered for binding to the antigen target. This site selectivity is achieved by targeting the carbohydrate domains present on essentially all IgG antibodies regardless of isotype and host species. Once azido–modified, a variety of labeled SiteClick sDIBO alkynes are available for attachment to the antibody via Click chemistry at your choice of scale. This provides the flexibility to choose different labels for your antibody depending on your assay.

Features of the SiteClick Antibody Azido Modification Kit:
• Contains everything required to azido-modify any species or isotype of IgG antibody
• Easy-to-follow step-by-step protocol
• Highly efficient, site-specific, reproducible labeling chemistry results in high quality antibody conjugate

Learn more about SiteClick labeling technology ›

Custom SiteClick Antibody Labeling Service and sDIBO labels
If you have an antibody that is considered 'difficult to label' or has lost activity after labeling using a conventional method, please contact our custom service representatives to determine whether the SiteClick Antibody Labeling Service would be right for your antibody. We offer complete custom SiteClick antibody labeling services with the option of multiple detection molecules including biotin, Alexa Fluor dyes, Qdot fluorophores, R-PE, chelates for PET imaging, and many others.

SiteClick™ Alexa Fluor™ 647 sDIBO Alkyne Invitrogen™

SiteClick labeled sDIBO alkynes for antibody labeling are optimized for easy attachment to azido modified antibodies using copper-free Click chemistry. This labeled sDIBO alkyne can be used with antibodies that have been modified using the SiteClick Antibody Azido Modification Kit or engineered to contain azido moieties. These labeled sDIBO alkynes are improved versions of our original DIBO cyclooctynes, yielding conjugates that are less 'sticky' and that produce lower signal background in biological samples. This modular labeling system gives you the option to choose different fluorescent labels for your antibody and attach another molecule via streptavidin or your own molecule via amine-reactive or amine-containing moieties depending on your assay.

Learn more about SiteClick labeling technology ›

Custom SiteClick Antibody Labeling Service and sDIBO labels
If you have an antibody that is considered 'difficult to label' or has lost activity after labeling using a conventional method, please contact our custom service representatives to determine whether the SiteClick Antibody Labeling Service would be right for your antibody. We offer complete custom SiteClick antibody labeling services with the option of multiple detection molecules including biotin, Alexa Fluor dyes, Qdot fluorophores, R-PE, chelates for PET imaging, and many others.

SiteClick™ Biotin sDIBO Alkyne Invitrogen™

SiteClick labeled sDIBO alkynes for antibody labeling are optimized for easy attachment to azido modified antibodies using copper-free Click chemistry. This labeled sDIBO alkyne can be used with antibodies that have been modified using the SiteClick Antibody Azido Modification Kit or engineered to contain azido moieties. These labeled sDIBO alkynes are improved versions of our original DIBO cyclooctynes, yielding conjugates that are less 'sticky' and that produce lower signal background in biological samples. This modular labeling system gives you the option to choose different fluorescent labels for your antibody and attach another molecule via streptavidin or your own molecule via amine-reactive or amine-containing moieties depending on your assay.

Learn more about SiteClick labeling technology ›

Custom SiteClick Antibody Labeling Service and sDIBO labels
If you have an antibody that is considered 'difficult to label' or has lost activity after labeling using a conventional method, please contact our custom service representatives to determine whether the SiteClick Antibody Labeling Service would be right for your antibody. We offer complete custom SiteClick antibody labeling services with the option of multiple detection molecules including biotin, Alexa Fluor dyes, Qdot fluorophores, R-PE, chelates for PET imaging, and many others.

SiteClick™ pHrodo™ iFL Red sDIBO Alkyne Invitrogen™

SiteClick labeled sDIBO alkynes for antibody labeling are optimized for easy attachment to azido modified antibodies using copper-free Click chemistry. This labeled sDIBO alkyne can be used with antibodies that have been modified using the SiteClick Antibody Azido Modification Kit or engineered to contain azido moieties. These labeled sDIBO alkynes are improved versions of our original DIBO cyclooctynes, yielding conjugates that are less 'sticky' and that produce lower signal background in biological samples. This modular labeling system gives you the option to choose different fluorescent labels for your antibody and attach another molecule via streptavidin or your own molecule via amine-reactive or amine-containing moieties depending on your assay.

The increase in fluorescence of pHrodo iFL red dye as the pH changes from basic to acidic correlates with the acidification of intracellular vesicles, making it a valuable tool for the study of antibody internalization via endocytosis or phagocytosis and regulation by environmental factors, drugs, or pathogens.

Learn more about SiteClick labeling technology ›
Learn more about IFL pHrodo antibody internalization ›

Custom SiteClick Antibody Labeling Service and sDIBO labels
If you have an antibody that is considered 'difficult to label' or has lost activity after labeling using a conventional method, please contact our custom service representatives to determine whether the SiteClick Antibody Labeling Service would be right for your antibody. We offer complete custom SiteClick antibody labeling services with the option of multiple detection molecules including biotin, Alexa Fluor dyes, Qdot fluorophores, R-PE, chelates for PET imaging, and many others.

SiteClick™ Alexa Fluor™ 488 sDIBO Alkyne Invitrogen™

SiteClick labeled sDIBO alkynes for antibody labeling are optimized for easy attachment to azido modified antibodies using copper-free Click chemistry. This labeled sDIBO alkyne can be used with antibodies that have been modified using the SiteClick Antibody Azido Modification Kit or engineered to contain azido moieties. These labeled sDIBO alkynes are improved versions of our original DIBO cyclooctynes, yielding conjugates that are less 'sticky' and that produce lower signal background in biological samples. This modular labeling system gives you the option to choose different fluorescent labels for your antibody and attach another molecule via streptavidin or your own molecule via amine-reactive or amine-containing moieties depending on your assay.

Learn more about SiteClick labeling technology ›

Custom SiteClick Antibody Labeling Service and sDIBO labels
If you have an antibody that is considered 'difficult to label' or has lost activity after labeling using a conventional method, please contact our custom service representatives to determine whether the SiteClick Antibody Labeling Service would be right for your antibody. We offer complete custom SiteClick antibody labeling services with the option of multiple detection molecules including biotin, Alexa Fluor dyes, Qdot fluorophores, R-PE, chelates for PET imaging, and many others.

K-AcylStat™ Panel, SNAP-ChIP™ Spike-in Invitrogen™

SNAP-ChIP (Sample Normalization and Antibody Profiling Chromatin ImmunoPrecipitation), a proprietary technology from EpiCypher, uses DNA-barcoded designer nucleosomes bearing distinct post-translational modifications as spike-in ChIP controls.

• Homogenous, fully defined standards faithfully represent target mononucleosomes in the experimental sample
• Nucleosomes are subjected to rigorous quality control for lot-to-lot consistency
• Unique DNA barcodes can be distinguished from experimental sample genomes
• Spike-ins provide a direct readout of antibody performance in ChIP
• Ability to monitor technical variability between samples
• Defined standards enable universal normalization across experiments

SNAP-ChIP panels are directly compatible with your current ChIP workflow, with semi-synthetic nucleosomes bearing the post-translational modification interest immunoprecipitated and processed alongside sample chromatin.

Users can directly monitor antibody capability (specificity and enrichment) in a ChIP experiment by measuring the recovery of on- and off-target nucleosomes. The SNAP spike-ins can be further used as a defined standard to normalize your ChIP data, thereby controlling for technical variability across experiments.

The K-AcylStat Panel is a lysine acylation status panel that consists of a pool of nucleosomes carrying twenty-two well-studied, disease relevant lysine acylation marks on histones H2A, H3, and H4 (see user manual for specifics) plus an unmodified control. Further, each of these nucleosomes is wrapped by two independent DNA barcodes (46 total barcodes in the panel), allowing for an internal technical replicate in each ChIP reaction. A single spike-in of the panel allows users to check antibody specificity by examining the post-IP recovery of on- versus off-target SNAP-ChIP nucleosomes, supporting the generation of high quality ChIP data.

K-MetStat™ Panel, SNAP-ChIP™ Spike-in Invitrogen™

SNAP-ChIP (Sample Normalization and Antibody Profiling Chromatin ImmunoPrecipitation), a proprietary technology from EpiCypher, uses DNA-barcoded semi-syntheic nucleosomes bearing distinct post-translational as spike-in ChIP controls.

• Homogenous, fully defined standards faithfully represent target mononucleosomes in the experimental sample
• Nucleosomes are subjected to rigorous quality control for lot-to-lot consistency
• Unique DNA barcodes can be distinguished from experimental sample genomes
• Spike-ins provide a direct readout of antibody performance in ChIP
• Ability to monitor technical variability between samples
• Defined standards enable universal normalization across experiments

SNAP-ChIP panels are directly compatible with your current ChIP workflow, with semi-synthetic nucleosomes bearing the post-translational modification of interest immunoprecipitated and processed alongside sample chromatin.

Users can directly monitor antibody capability (specificity and enrichment) in a ChIP experiment by measuring the recovery of on- and off-target nucleosomes. The SNAP spike-ins can be further used as a defined standard to normalize your ChIP data, thereby controlling for technical variability across experiments.

The K-MetStat Panel is a lysine methylation status panel that consists of a pool of nucleosomes carrying fifteen well-studied, disease relevant lysine methyl marks on histones H3 and H4 (mono-, di- and tri-methylated H3K4, H3K9, H3K27, H3K36, and H4K20) plus an unmodified control. Further, each of these nucleosomes is wrapped by two independent DNA barcodes (32 total barcodes in the panel), allowing for an internal technical replicate in each ChIP reaction. A single spike-in of the panel allows users to check antibody specificity by examining the post-IP recovery of on- versus off-target SNAP-ChIP nucleosomes, supporting the generation of high quality ChIP data.

OncoStat™ Panel, SNAP-ChIP™ Spike-in Invitrogen™

SNAP-ChIP (Sample Normalization and Antibody Profiling Chromatin ImmunoPrecipitation), a proprietary technology from EpiCypher, uses DNA-barcoded semi-synthetic nucleosomes bearing distinct histone mutations as spike-in ChIP controls.

• Homogenous, fully defined standards faithfully represent target mononucleosomes in the experimental sample
• Nucleosomes are subjected to rigorous quality control for lot-to-lot consistency
• Unique DNA barcodes can be distinguished from experimental sample genomes
• Spike-ins provide a direct readout of antibody performance in ChIP
• Ability to monitor technical variability between samples
• Defined standards enable universal normalization across experiments

SNAP-ChIP panels are directly compatible with your current ChIP workflow, with semi-synthetic nucleosomes bearing the mutation of interest immunoprecipitated and processed alongside sample chromatin.

Users can directly monitor antibody capability (specificity and enrichment) in a ChIP experiment by measuring the recovery of on- and off-target nucleosomes. The SNAP spike-ins can be further used as a defined standard to normalize your ChIP data, thereby controlling for technical variability across experiments.

The OncoStat Panel consists of a pool of nucleosomes harboring seven well-studied histone H3.3 mutations that have been implicated in cancer (histone H3.3K4M, H3.3K9M, H3.3K27M, H3.3G34R, H3.3G34V, H3.3G34W and H3.3K36M) plus a wild-type H3.3 control. Further, each of these eight nucleosomes is wrapped by two independent DNA barcodes (16 total barcodes in the panel), allowing for an internal technical replicate in each ChIP reaction. A single spike-in of the panel allows users to check antibody specificity by examining the post-IP recovery of on- versus off-target SNAP-ChIP nucleosomes, supporting the generation of high quality ChIP data.

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