Flow Cytometry Compensation, Counting and Calibration

Reagents and kits for flow cytometer instrument calibration, sample compensation beads and kits, cell counting standards, and other reagents needed to set your instrument up.
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eBioscience™ Fixation/Permeabilization Diluent Invitrogen™

The Fixation/Permeabilization Diluent is intended to be used with antibodies to transcription factors and nuclear proteins, such as Foxp3 and Ki-67, as well as cytokines and chemokines. This must be used in combination with the Fixation/Permeabilization Concentrate (cat.

eBioscience™ Flow Cytometry Staining Buffer Invitrogen™

Our Flow Cytometry Staining Buffer is designed for use in immunofluorescent staining protocols of cells in suspension. This Flow Cytometry Staining Buffer is a buffered saline solution containing fetal bovine serum and sodium azide (0.09%) as a preservative.

eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set Invitrogen™

The Foxp3 Transcription Factor Staining Buffer Set has been formulated and optimized for staining with antibodies to transcription factors and nuclear proteins, such as Foxp3 and Ki-67, as well as cytokines and chemokines.

eBioscience™ Cell Stimulation Cocktail (500X) Invitrogen™

The Cell Stimulation Cocktail (500X) is a cocktail of phorbol 12-myristate 13-acetate (PMA) and ionomycin. Treatment with PMA and ionomycin is sufficient to induce activation of many cell types to produce cytokines.

eBioscience™ Foxp3 / Transcription Factor Fixation/Permeabilization Concentrate and Diluent Invitrogen™

The Foxp3 Transcription Factor Fixation/Permeabilization Concentrate and Diluent solutions for staining with antibodies to transcription factors and nuclear proteins, such as Foxp3 and Ki-67, as well as cytokines and chemokines.

eBioscience™ Mouse Intracellular (cytoplasmic/cytokine) Protein Flow Cytometry Workflow Kit Invitrogen™

The eBioscience Mouse Intracellular (cytoplasmic/cytokine) Protein Flow Cytometry Workflow Kit contains all the buffers, blockers, and beads to complete a standard flow cytometry experiment for staining intracellular cytoplasmic and cytokine targets in mouse cell suspensions.

8000 Series Silica Particle Size Standards Thermo Scientific™

Provide greater durability and chemical stability when using solvents with Thermo Scientific™ 8000 Series Silica Particle Size Standards, monodisperse silica microspheres used in instrument calibration and as spacers.

Cell Sorting Set-up Beads (for red lasers) Invitrogen™

Molecular Probes™ Cell Sorting Set-up Beads are reliable standards for flow cytometry sorter instrument setup and calibration. The Cell Sorting Set-up Beads approximate the size, emission wavelength, and intensity of many biological samples, and so can be used to calibrate a flow cytometer’s cell...

CountBright™ Plus Absolute Counting Beads Invitrogen™

CountBright Plus Absolute Counting Beads are a calibrated suspension of ∼4 µm beads that encapsulate dyes that are excitable across a wide range of UV-IR wavelengths and brightly fluoresce in the emission range of 385-860 nm.

Cell Sorting Set-up Beads (for blue lasers) Invitrogen™

Molecular Probes™ Cell Sorting Set-up Beads are reliable standards for flow cytometry sorter instrument setup and calibration. The Cell Sorting Set-up Beads approximate the size, emission wavelength, and intensity of many biological samples, and so can be used to calibrate a flow cytometer’s cell...

CountBright™ Absolute Counting Beads, for flow cytometry Invitrogen™

CountBright™ absolute counting beads are a calibrated suspension of microspheres that are brightly fluorescent across a wide range of excitation and emission wavelengths (UV to 635 nm excitation and 385-800 nm emission).

CD44 Alexa Fluor™ 488 Conjugate Kit for Live Cell Imaging Invitrogen™

Molecular Probes™ human pluripotent stem cell (PSC) live imaging kits provide reagents for superior imaging of live human PSCs. This CD44 Alexa Fluor™ 488 Conjugate Kit includes an optimally labeled primary antibody paired with reduced-background imaging medium (FluoroBrite™ DMEM) for the specific...

eBioscience™ Essential Human T-Cell Phenotyping Kit Invitrogen™

The Invitrogen eBioscience Essential Human T-Cell Phenotyping Kit is designed for flow cytometry-based identity testing of T-cells. It leverages high-quality eBioscience antibodies and isotype controls and includes a ready-to-use protocol for experimental setup, gating strategy, and analysis.

UltraComp eBeads™ Compensation Beads Invitrogen™

UltraComp eBeads™ react with antibodies of mouse, rat, and hamster origin, and are immunoglobulin light chain-independent. They are designed for use in compensation with all fluorochromes excited by ultraviolet (355 nm) violet (405 nm), blue (488 nm), green (532 nm), yellow-green (561 nm), and red...

OneComp eBeads™ Compensation Beads Invitrogen™

OneComp eBeads react with antibodies of mouse, rat and hamster origin, and are immunoglobulin light chain-independent. The beads are spherical particles that can be stained with individual fluorochrome-conjugated antibodies for use as single-color compensation controls.
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