Flow Cytometry Compensation, Counting and Calibration

Reagents and kits for flow cytometer instrument calibration, sample compensation beads and kits, cell counting standards, and other reagents needed to set your instrument up.
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eBioscience™ Fixation/Permeabilization Diluent Invitrogen™

The Fixation/Permeabilization Diluent is intended to be used with antibodies to transcription factors and nuclear proteins, such as Foxp3 and Ki-67, as well as cytokines and chemokines. This must be used in combination with the Fixation/Permeabilization Concentrate (cat.

eBioscience™ Flow Cytometry Staining Buffer Invitrogen™

Our Flow Cytometry Staining Buffer is designed for use in immunofluorescent staining protocols of cells in suspension. This Flow Cytometry Staining Buffer is a buffered saline solution containing fetal bovine serum and sodium azide (0.09%) as a preservative.

eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set Invitrogen™

The Foxp3 Transcription Factor Staining Buffer Set has been formulated and optimized for staining with antibodies to transcription factors and nuclear proteins, such as Foxp3 and Ki-67, as well as cytokines and chemokines.

eBioscience™ Cell Stimulation Cocktail (500X) Invitrogen™

The Cell Stimulation Cocktail (500X) is a cocktail of phorbol 12-myristate 13-acetate (PMA) and ionomycin. Treatment with PMA and ionomycin is sufficient to induce activation of many cell types to produce cytokines.

eBioscience™ Foxp3 / Transcription Factor Fixation/Permeabilization Concentrate and Diluent Invitrogen™

The Foxp3 Transcription Factor Fixation/Permeabilization Concentrate and Diluent solutions for staining with antibodies to transcription factors and nuclear proteins, such as Foxp3 and Ki-67, as well as cytokines and chemokines.

eBioscience™ Mouse Intracellular (cytoplasmic/cytokine) Protein Flow Cytometry Workflow Kit Invitrogen™

The eBioscience Mouse Intracellular (cytoplasmic/cytokine) Protein Flow Cytometry Workflow Kit contains all the buffers, blockers, and beads to complete a standard flow cytometry experiment for staining intracellular cytoplasmic and cytokine targets in mouse cell suspensions.

8000 Series Silica Particle Size Standards Thermo Scientific™

Provide greater durability and chemical stability when using solvents with Thermo Scientific™ 8000 Series Silica Particle Size Standards, monodisperse silica microspheres used in instrument calibration and as spacers.

Cell Sorting Set-up Beads (for red lasers) Invitrogen™

Molecular Probes™ Cell Sorting Set-up Beads are reliable standards for flow cytometry sorter instrument setup and calibration. The Cell Sorting Set-up Beads approximate the size, emission wavelength, and intensity of many biological samples, and so can be used to calibrate a flow cytometer’s cell...

CountBright™ Plus Absolute Counting Beads Invitrogen™

CountBright Plus Absolute Counting Beads are a calibrated suspension of ∼4 µm beads that encapsulate dyes that are excitable across a wide range of UV-IR wavelengths and brightly fluoresce in the emission range of 385-860 nm.

Cell Sorting Set-up Beads (for blue lasers) Invitrogen™

Molecular Probes™ Cell Sorting Set-up Beads are reliable standards for flow cytometry sorter instrument setup and calibration. The Cell Sorting Set-up Beads approximate the size, emission wavelength, and intensity of many biological samples, and so can be used to calibrate a flow cytometer’s cell...

CountBright™ Absolute Counting Beads, for flow cytometry Invitrogen™

CountBright™ absolute counting beads are a calibrated suspension of microspheres that are brightly fluorescent across a wide range of excitation and emission wavelengths (UV to 635 nm excitation and 385-800 nm emission).

CD44 Alexa Fluor™ 488 Conjugate Kit for Live Cell Imaging Invitrogen™

Molecular Probes™ human pluripotent stem cell (PSC) live imaging kits provide reagents for superior imaging of live human PSCs. This CD44 Alexa Fluor™ 488 Conjugate Kit includes an optimally labeled primary antibody paired with reduced-background imaging medium (FluoroBrite™ DMEM) for the specific...

eBioscience™ Essential Human T-Cell Phenotyping Kit Invitrogen™

The Invitrogen eBioscience Essential Human T-Cell Phenotyping Kit is designed for flow cytometry-based identity testing of T-cells. It leverages high-quality eBioscience antibodies and isotype controls and includes a ready-to-use protocol for experimental setup, gating strategy, and analysis.

UltraComp eBeads™ Compensation Beads Invitrogen™

UltraComp eBeads™ react with antibodies of mouse, rat, and hamster origin, and are immunoglobulin light chain-independent. They are designed for use in compensation with all fluorochromes excited by ultraviolet (355 nm) violet (405 nm), blue (488 nm), green (532 nm), yellow-green (561 nm), and red...

OneComp eBeads™ Compensation Beads Invitrogen™

OneComp eBeads react with antibodies of mouse, rat and hamster origin, and are immunoglobulin light chain-independent. The beads are spherical particles that can be stained with individual fluorochrome-conjugated antibodies for use as single-color compensation controls.

eBioscience™ Mouse Regulatory T Cell Staining Kit #2 Invitrogen™

The FJK-16s antibody reacts with mouse/rat Foxp3 also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3 a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified...

eBioscience™ Human Th17 Cytokine Staining Panel Invitrogen™

This human Th17 cytokine staining panel includes all reagents needed for simultaneous flow cytometric detection of all the major cytokines produced by the Th17 lineage: IL-17A, IL-17F, IL-21 and IL-22.

AbC™ Total Antibody Compensation Bead Kit Invitrogen™

The AbC™ Total Antibody Compensation Bead Kit provides a consistent, accurate, and simple-to-use technique for the setting of flow cytometry compensation when using fluorochrome-conjugated mouse, rat, hamster, or rabbit antibodies with any laser in flow cytometry.

GFP BrightComp eBeads™ Compensation Bead Kit Invitrogen™

GFP BrightComp eBeads provide a consistent, accurate and simple-to-use technique for setting flow cytometry compensation when using GFP-expressing samples. The single-color stained beads are excited by a blue (488 nm) laser.

ArC™ Amine Reactive Compensation Bead Kit (for use with LIVE/DEAD™ Fixable dead cell stain kits) Invitrogen™

ArC™ Amine Reactive Compensation Bead Kit is a bead-based compensation tool specifically optimized for use with LIVE/DEAD™ Fixable dead cell stain kits. • Ready-to-use control • Eliminates the hassle of heat-treating cells as a control, saving precious sample • Enables accurate and consistent...

Alignflow™ Flow Cytometry Alignment Beads for Red Lasers, 2.5 µm Invitrogen™

Molecular Probes™ AlignFlow™ flow cytometry alignment beads are reliable references for aligning, focusing, and calibrating flow cytometers.The 2.5 µm Alignflow™ Flow Cytometry Alignment Beads for Red Lasers are fluorescent-dye infused microspheres that are excitable at 630–640 nm with emission in...

eBioscience™ Mouse Hematopoietic Lineage Biotin Panel Invitrogen™

The eBioscience Mouse Hematopoietic Lineage Flow Panel contains 5 biotinylated antibodies that can be used to identify, enrich and/or deplete cells committed to the T, B, NK, myeloid and erythroid lineages based on their cell surface antigen expression.

eBioscience™ Mouse Regulatory T Cell Staining Kit #3 Invitrogen™

The FJK-16s antibody reacts with mouse/rat Foxp3 also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3 a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified...

eBioscience™ Mouse Th17 Cytokine Staining Panel Invitrogen™

This mouse Th17 cytokine staining panel includes all reagents needed for simultaneous flow cytometric detection of all the major cytokines produced by the Th17 lineage: IL-17A, IL-17F, IL-21 and IL-22.

eZKine™ 4-Color Compensation Control Kit Invitrogen™

This eZKine™ 4-Color Compensation Control Kit is designed for compensation setup with eZKine™ Whole Blood Intracellular Cytokine Kits. It contains the necessary components to create single-color compensation controls for use with eZKine™ cocktails, including OneComp eBeads and 4 vials of antibody...

eBioscience™ Human Regulatory T Cell Staining Kit #2 Invitrogen™

This Human Regulatory T cell Staining Kit contains all of the buffers and monoclonal antibodies for CD4, CD25, and Foxp3 necessary to successfully stain and identify regulatory T cells from human peripheral blood cells.

eZKine™ CD8 Activation 1 Whole Blood Intracellular Cytokine Kit Invitrogen™

This eZKine CD8 Activation 1 Whole Blood Intracellular Cytokine Kit is designed to rapidly identify cytokine producing T lymphocytes of the CD8+ T cell lineage after stimulation of whole peripheral blood samples.

eBioscience™ Mouse Regulatory T Cell Staining Kit #1 Invitrogen™

The FJK-16s antibody reacts with mouse/rat Foxp3 also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3 a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified...

eBioscience™ Human Regulatory T Cell Staining Kit #3 Invitrogen™

This Human Regulatory T cell Staining Kit contains all of the buffers and monoclonal antibodies for CD4, CD25, and Foxp3 necessary to successfully stain and identify regulatory T cells from human peripheral blood cells.

Fixation Medium (Medium A) Invitrogen™

Fixation Medium (Medium A) is a component of the FIX & PERM™ Cell Permeabilization Kit. The kit is used in flow cytometry for fixing and permeabilizing cells in suspension, allowing access of antibody to intracellular targets without sacrificing scatter characteristics.

Lysing Solution, Whole Blood Lysing Solution Invitrogen™

Invitrogen Cal-Lyse™ is a lysing solution formulated to enable the lysis of erythrocytes in samples of anticoagulated human peripheral blood. Invitrogen Cal-Lyse™ lysing solution is intended to be used as an aid in the enumeration of leukocytes that have been stained with monoclonal antibodies for...

Flow Cytometry Size Calibration Kit (nonfluorescent microspheres) Invitrogen™

Our Flow Cytometry Size Calibration Kit has nonfluorescent particle-size calibration standards that provide a simple, accurate way to determine cell sizes by flow cytometry. The kit contains six suspensions of highly uniform polystyrene microspheres with the following diameters: 1.0 µm, 2.0 µm, 4.

Cell Sorting Set-up Beads (for green-yellow lasers) Invitrogen™

Molecular Probes™ Cell Sorting Set-up Beads are reliable standards for flow cytometry sorter instrument setup and calibration. The Cell Sorting Set-up Beads approximate the size, emission wavelength, and intensity of many biological samples, and so can be used to calibrate a flow cytometer’s cell...

K-MetStat™ Panel, SNAP-ChIP™ Spike-in Invitrogen™

SNAP-ChIP (Sample Normalization and Antibody Profiling Chromatin ImmunoPrecipitation), a proprietary technology from EpiCypher, uses DNA-barcoded semi-syntheic nucleosomes bearing distinct post-translational as spike-in ChIP controls.

K-AcylStat™ Panel, SNAP-ChIP™ Spike-in Invitrogen™

SNAP-ChIP (Sample Normalization and Antibody Profiling Chromatin ImmunoPrecipitation), a proprietary technology from EpiCypher, uses DNA-barcoded designer nucleosomes bearing distinct post-translational modifications as spike-in ChIP controls.

eBioscience™ Mouse Intracellular (nuclear/transcription factor) Protein Flow Cytometry Workflow Kit Invitrogen™

The eBioscience Mouse Intracellular (nuclear/transcription factor) Protein Flow Cytometry Workflow Kit contains all the buffers, blockers, and beads to complete a standard flow cytometry experiment for staining intracellular nuclear and transcription factor targets in mouse cell suspensions.

eBioscience™ Human Intracellular (cytoplasmic/cytokine) Protein Flow Cytometry Workflow Kit Invitrogen™

The eBioscience Human Intracellular (cytoplasmic/cytokine) Protein Flow Cytometry Workflow Kit contains all the buffers, blockers, and beads to complete a standard flow cytometry experiment for staining intracellular cytoplasmic and cytokine targets in human cell suspensions.

eBioscience™ Essential Human Treg Phenotyping Panel & Staining Buffers Invitrogen™

The Invitrogen eBioscience Essential Human Treg Phenotyping Panel & Staining Buffers is designed for flow cytometry-based identity testing of regulatory T-cells (Tregs). It leverages high quality eBioscience antibodies and fixation/permeabilization reagents (for the intracellular marker FOXP3) and...

eBioscience™ Essential Human Th1/Th17 Phenotyping Panel with Staining & Stimulation Reagents Invitrogen™

The Invitrogen eBioscience Essential Human Th1/Th17 Phenotyping Panel with Staining & Stimulation Reagents is designed for flow cytometry-based identity testing of Type 1 and Type 17 T-helper cells (Th1/Th17).

eBioscience™ Fixation/Permeabilization Concentrate Invitrogen™

The Fixation/Permeabilization Concentrate is intended to be used with antibodies to transcription factors and nuclear proteins, such as Foxp3 and Ki-67, as well as cytokines and chemokines. This must be used in combination with the Fixation/Permeabilization Diluent (cat.

eBioscience™ Cell Stimulation Cocktail (plus protein transport inhibitors) (500X) Invitrogen™

The Cell Stimulation Cocktail (plus protein transport inhibitors) (500X) is a cocktail of phorbol 12-myristate 13-acetate (PMA), ionomycin, brefeldin A and monensin. Treatment with PMA and ionomycin is sufficient to induce activation of many cell types to produce cytokines.

eBioscience™ IC Fixation Buffer Invitrogen™

The Intracellular (IC) Fixation Buffer can be used to fix cells prior to permeabilization with the Permeabilization Buffer (cat. 00-8333), when performing intracellular staining of antigens located in the cytoplasm and secretory pathway, such as cytokines, chemokines and growth factors.

eBioscience™ Permeabilization Buffer (10X) Invitrogen™

The Permeabilization Buffer (10X), in conjunction with the Intracellular (IC) Fixation Buffer (cat. 00-8222) or Foxp3 Transcription Factor Fixation/Permeabilization Concentrate and Diluent (cat. 00-5521), can be used to fix and permeabilize cells prior to performing intracellular staining of...

eBioscience™ Mouse Cytokine Positive Control Cells Invitrogen™

The Mouse Cytokine Positive Control Cells are recommended for use as positive control cells when establishing an intracellular cytokine staining protocol. Mouse splenocytes were stimulated with ConA (3 µg/mL) for two days, followed by recombinant IL-2 (20 ng/mL) and IL-4 (20 ng/mL) for three days.

123count eBeads™ Counting Beads Invitrogen™

123count eBeads are intended for use in absolute counting of cells or other particles by flow cytometry. These are 7 μm microparticles containing encapsulated dyes compatible with blue (488 nm) and violet (405 nm) excitation sources and emitting fluorescence between approximately 500 nm and 750 nm.

Alignflow™ Flow Cytometry Alignment Beads for Blue Lasers, 6.0 µm Invitrogen™

Molecular Probes™ AlignFlow™ flow cytometry alignment beads are reliable references for aligning, focusing, and calibrating flow cytometers.The 6.0 µm Alignflow™ Flow Cytometry Alignment Beads for Blue Lasers are fluorescent-dye infused microspheres that are excitable at 488 nm with emission in the...

Alignflow™ Flow Cytometry Alignment Beads for UV Lasers, 2.5 µm Invitrogen™

Molecular Probes™ AlignFlow™ flow cytometry alignment beads are reliable references for aligning, focusing, and calibrating flow cytometers.The 2.5 µm Alignflow™ Flow Cytometry Alignment Beads for UV Lasers are fluorescent-dye infused microspheres that are optimally excitable between 350-370 nm with...

Alignflow™ Flow Cytometry Alignment Beads for Red Lasers, 6.0 µm Invitrogen™

Molecular Probes™ AlignFlow™ flow cytometry alignment beads are reliable references for aligning, focusing, and calibrating flow cytometers.The 6.0 µm Alignflow™ Flow Cytometry Alignment Beads for Red Lasers are fluorescent-dye infused microspheres that are excitable at 630–640 nm with emission in...

Alignflow™ Flow Cytometry Alignment Beads for UV Lasers, 6.0 µm Invitrogen™

Molecular Probes™ AlignFlow™ flow cytometry alignment beads are reliable references for aligning, focusing, and calibrating flow cytometers.The 6.0 µm AlignFlow™ Flow Cytometry Alignment Beads for UV Lasers are fluorescent-dye infused microspheres that are optimally excitable between 350–370 nm with...

Alignflow™ Flow Cytometry Alignment Beads for Blue Lasers, 2.5 µm Invitrogen™

Molecular Probes™ AlignFlow™ flow cytometry alignment beads are reliable references for aligning, focusing, and calibrating flow cytometers.The 2.5 µm Alignflow™ Flow Cytometry Alignment Beads for Blue Lasers are fluorescent-dye infused microspheres that are excitable at 488 nm with emission in the...

eBioscience™ Human Intracellular (nuclear/transcription factor) Protein Flow Cytometry Workflow Kit Invitrogen™

The eBioscience Human Intracellular (nuclear/transcription factor) Protein Flow Cytometry Workflow Kit contains all the buffers, blockers, and beads to complete a standard flow cytometry experiment for staining intracellular nuclear and transcription factor targets in human cell suspensions.

eBioscience™ Human Cell Surface Flow Cytometry Workflow Kit Invitrogen™

The eBioscience Human Cell Surface Flow Cytometry Workflow Kit contains all the buffers, blockers, and beads to complete a standard flow cytometry experiment for staining cell surface targets on human cell suspensions.

AccuCheck Counting Beads Invitrogen™

AccuCheck Counting Beads are an efficient single-platform method for absolute cell counts that combines the advantages of direct flow cytometric immunophenotyping with the use of two different fluorescent beads (A and B beads).

AbC™ Anti-Mouse Bead Kit Invitrogen™

The AbC™ Anti-Mouse Antibody Compensation Bead Kit provides a consistent, accurate, and simple-to-use technique for the setting of flow cytometry compensation when using fluorochrome-conjugated mouse antibodies with any laser in flow cytometry.

eBioscience™ Essential Human T-Cell Phenotyping Panel Invitrogen™

The Invitrogen eBioscience Essential Human T-Cell Phenotyping Panel is designed for flow cytometry-based identity testing of T-cells. It leverages high quality eBioscience antibodies and a ready-to-use protocol for experimental setup, gating strategy, and analysis.

Cell Sorting Set-up Beads (for UV lasers) Invitrogen™

Molecular Probes™ Cell Sorting Set-up Beads are reliable standards for flow cytometry sorter instrument setup and calibration. The Cell Sorting Set-up Beads approximate the size, emission wavelength, and intensity of many biological samples, and so can be used to calibrate a flow cytometer’s cell...

OncoStat™ Panel, SNAP-ChIP™ Spike-in Invitrogen™

SNAP-ChIP (Sample Normalization and Antibody Profiling Chromatin ImmunoPrecipitation), a proprietary technology from EpiCypher, uses DNA-barcoded semi-synthetic nucleosomes bearing distinct histone mutations as spike-in ChIP controls.

Flow Cytometry Sub-micron Particle Size Reference Kit Invitrogen™

The Flow Cytometry Sub-micron Particle Size Reference Kit provides six sizes of green-fluorescent beads (ranging in diameter from 0.02 µm to 2.0 µm) packaged in individual vials. Each bead is uniform in size, and the green-fluorescent dyes are localized within the bead, which makes the beads...

eBioscience™ Human Regulatory T Cell Staining Kit Invitrogen™

This Human Regulatory T cell Staining Kit contains all of the buffers and monoclonal antibodies for CD4, CD25, and Foxp3 necessary to successfully stain and identify regulatory T cells from human peripheral blood cells.

AbC™ Anti-Rat/Hamster Bead Kit Invitrogen™

The AbC™ Anti-Rat/Hamster Antibody Compensation Bead Kit provides a consistent, accurate, and simple-to-use technique for the setting of flow cytometry compensation when using fluorochrome-conjugated to rat or hamster antibodies with any laser in flow cytometry.
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