Flow Cytometry Compensation, Counting and Calibration

Reagents and kits for flow cytometer instrument calibration, sample compensation beads and kits, cell counting standards, and other reagents needed to set your instrument up.
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eBioscience™ Mouse Cytokine Positive Control Cells Invitrogen™

The Mouse Cytokine Positive Control Cells are recommended for use as positive control cells when establishing an intracellular cytokine staining protocol. Mouse splenocytes were stimulated with ConA (3 µg/mL) for two days, followed by recombinant IL-2 (20 ng/mL) and IL-4 (20 ng/mL) for three days.

eBioscience™ Cell Stimulation Cocktail (plus protein transport inhibitors) (500X) Invitrogen™

The Cell Stimulation Cocktail (plus protein transport inhibitors) (500X) is a cocktail of phorbol 12-myristate 13-acetate (PMA), ionomycin, brefeldin A and monensin. Treatment with PMA and ionomycin is sufficient to induce activation of many cell types to produce cytokines.

eBioscience™ Fixation/Permeabilization Concentrate Invitrogen™

The Fixation/Permeabilization Concentrate is intended to be used with antibodies to transcription factors and nuclear proteins, such as Foxp3 and Ki-67, as well as cytokines and chemokines. This must be used in combination with the Fixation/Permeabilization Diluent (cat.

eBioscience™ Permeabilization Buffer (10X) Invitrogen™

The Permeabilization Buffer (10X), in conjunction with the Intracellular (IC) Fixation Buffer (cat. 00-8222) or Foxp3 Transcription Factor Fixation/Permeabilization Concentrate and Diluent (cat. 00-5521), can be used to fix and permeabilize cells prior to performing intracellular staining of...

eBioscience™ IC Fixation Buffer Invitrogen™

The Intracellular (IC) Fixation Buffer can be used to fix cells prior to permeabilization with the Permeabilization Buffer (cat. 00-8333), when performing intracellular staining of antigens located in the cytoplasm and secretory pathway, such as cytokines, chemokines and growth factors.

123count eBeads™ Counting Beads Invitrogen™

123count eBeads are intended for use in absolute counting of cells or other particles by flow cytometry. These are 7 μm microparticles containing encapsulated dyes compatible with blue (488 nm) and violet (405 nm) excitation sources and emitting fluorescence between approximately 500 nm and 750 nm.

Alignflow™ Flow Cytometry Alignment Beads for UV Lasers, 6.0 µm Invitrogen™

Molecular Probes™ AlignFlow™ flow cytometry alignment beads are reliable references for aligning, focusing, and calibrating flow cytometers.The 6.0 µm AlignFlow™ Flow Cytometry Alignment Beads for UV Lasers are fluorescent-dye infused microspheres that are optimally excitable between 350–370 nm with...

Alignflow™ Flow Cytometry Alignment Beads for Blue Lasers, 2.5 µm Invitrogen™

Molecular Probes™ AlignFlow™ flow cytometry alignment beads are reliable references for aligning, focusing, and calibrating flow cytometers.The 2.5 µm Alignflow™ Flow Cytometry Alignment Beads for Blue Lasers are fluorescent-dye infused microspheres that are excitable at 488 nm with emission in the...

Alignflow™ Flow Cytometry Alignment Beads for Blue Lasers, 6.0 µm Invitrogen™

Molecular Probes™ AlignFlow™ flow cytometry alignment beads are reliable references for aligning, focusing, and calibrating flow cytometers.The 6.0 µm Alignflow™ Flow Cytometry Alignment Beads for Blue Lasers are fluorescent-dye infused microspheres that are excitable at 488 nm with emission in the...

Cell Sorting Set-up Beads (for UV lasers) Invitrogen™

Molecular Probes™ Cell Sorting Set-up Beads are reliable standards for flow cytometry sorter instrument setup and calibration. The Cell Sorting Set-up Beads approximate the size, emission wavelength, and intensity of many biological samples, and so can be used to calibrate a flow cytometer’s cell...

AbC™ Anti-Mouse Bead Kit Invitrogen™

The AbC™ Anti-Mouse Antibody Compensation Bead Kit provides a consistent, accurate, and simple-to-use technique for the setting of flow cytometry compensation when using fluorochrome-conjugated mouse antibodies with any laser in flow cytometry.

AbC™ Anti-Rat/Hamster Bead Kit Invitrogen™

The AbC™ Anti-Rat/Hamster Antibody Compensation Bead Kit provides a consistent, accurate, and simple-to-use technique for the setting of flow cytometry compensation when using fluorochrome-conjugated to rat or hamster antibodies with any laser in flow cytometry.

eBioscience™ Human Regulatory T Cell Whole Blood Staining Kit Invitrogen™

This Human Regulatory T Cell Whole Blood Staining Kit contains the buffers and monoclonal antibody necessary to successfully stain and identify Foxp3 cells in whole blood samples. The PCH101 monoclonal antibody reacts with the amino terminus of human Foxp3 also known as FORKHEAD BOX P3, SCURFIN, and...

OncoStat™ Panel, SNAP-ChIP™ Spike-in Invitrogen™

SNAP-ChIP (Sample Normalization and Antibody Profiling Chromatin ImmunoPrecipitation), a proprietary technology from EpiCypher, uses DNA-barcoded semi-synthetic nucleosomes bearing distinct histone mutations as spike-in ChIP controls.

eBioscience™ Human Intracellular (nuclear/transcription factor) Protein Flow Cytometry Workflow Kit Invitrogen™

The eBioscience Human Intracellular (nuclear/transcription factor) Protein Flow Cytometry Workflow Kit contains all the buffers, blockers, and beads to complete a standard flow cytometry experiment for staining intracellular nuclear and transcription factor targets in human cell suspensions.

eBioscience™ Human Cell Surface Flow Cytometry Workflow Kit Invitrogen™

The eBioscience Human Cell Surface Flow Cytometry Workflow Kit contains all the buffers, blockers, and beads to complete a standard flow cytometry experiment for staining cell surface targets on human cell suspensions.

eBioscience™ Mouse Cell Surface Staining Flow Cytometry Workflow Kit Invitrogen™

The eBioscience Mouse Cell Surface Flow Cytometry Workflow Kit contains all the buffers, blockers, and beads to complete a standard flow cytometry experiment for staining cell surface targets on mouse cell suspensions.

eBioscience™ Human Regulatory T Cell Staining Kit Invitrogen™

This Human Regulatory T cell Staining Kit contains all of the buffers and monoclonal antibodies for CD4, CD25, and Foxp3 necessary to successfully stain and identify regulatory T cells from human peripheral blood cells.

eBioscience™ Intracellular Fixation & Permeabilization Buffer (plus Brefeldin A) Invitrogen™

The eBioscience Fixation and Permeabilization Kit is designed for use in preparation of living cells for intracellular staining and flow cytometric analysis. A protein transport inhibitor (Brefeldin A) is included for treatment of cells during culture – to cause accumulation of cytokine protein at...

eZKine™ Th1/Th17 Whole Blood Intracellular Cytokine Kit Invitrogen™

This eZKine Th1/Th17 Whole Blood Intracellular Cytokine Kit is designed to rapidly identify cytokine producing T lymphocytes of the Th1 and Th17 lineages after stimulation of whole peripheral blood samples. Stimulation of whole blood in the presence of Protein Transport Inhibitor Cocktail (cat.

eBioscience™ Essential Human T-Cell Phenotyping Panel Invitrogen™

The Invitrogen eBioscience Essential Human T-Cell Phenotyping Panel is designed for flow cytometry-based identity testing of T-cells. It leverages high quality eBioscience antibodies and a ready-to-use protocol for experimental setup, gating strategy, and analysis.

eBioscience™ Essential Human Th1/Th17 Phenotyping Kit Invitrogen™

The Invitrogen eBioscience Essential Human Th1/Th17 Phenotyping Kit is designed for flow cytometry-based identity testing of Type 1 and Type 17 T–helper cells (Th1/Th17). It leverages high quality eBioscience antibodies, isotype controls, cell stimulation reagents (induces cytokine production), and...

eBioscience™ Essential Human Treg Phenotyping Kit Invitrogen™

The Invitrogen eBioscience Essential Human Treg Phenotyping Kit is designed for flow cytometry-based identity testing of regulatory T-cells (Tregs). It leverages high-quality eBioscience antibodies, isotype controls, and fixation/permeabilization reagents (for the intracellular marker FOXP3) and...

eBioscience™ Human Lysed Whole Blood Cell Flow Cytometry Workflow Kit Invitrogen™

The eBioscience Human Lysed Whole Blood Cell Flow Cytometry Workflow Kit contains all the buffers, blockers, and beads to complete a standard flow cytometry experiment for staining cell surface targets on human lysed whole blood.

Alignflow™ Flow Cytometry Alignment Beads for Red Lasers, 6.0 µm Invitrogen™

Molecular Probes™ AlignFlow™ flow cytometry alignment beads are reliable references for aligning, focusing, and calibrating flow cytometers.The 6.0 µm Alignflow™ Flow Cytometry Alignment Beads for Red Lasers are fluorescent-dye infused microspheres that are excitable at 630–640 nm with emission in...

Alignflow™ Flow Cytometry Alignment Beads for UV Lasers, 2.5 µm Invitrogen™

Molecular Probes™ AlignFlow™ flow cytometry alignment beads are reliable references for aligning, focusing, and calibrating flow cytometers.The 2.5 µm Alignflow™ Flow Cytometry Alignment Beads for UV Lasers are fluorescent-dye infused microspheres that are optimally excitable between 350-370 nm with...

Super Bright Complete Staining Buffer, eBioscience Invitrogen™

Super Bright Complete Staining Buffer, eBioscience, is designed for use as a supplement to Flow Cytometry Staining Buffer in immunofluorescent staining protocols of cells in suspension. Super Bright Complete Staining Buffer is only necessary when using more than one polymer dye-conjugated antibody...

AccuCheck Counting Beads Invitrogen™

AccuCheck Counting Beads are an efficient single-platform method for absolute cell counts that combines the advantages of direct flow cytometric immunophenotyping with the use of two different fluorescent beads (A and B beads).

Flow Cytometry Sub-micron Particle Size Reference Kit Invitrogen™

The Flow Cytometry Sub-micron Particle Size Reference Kit provides six sizes of green-fluorescent beads (ranging in diameter from 0.02 µm to 2.0 µm) packaged in individual vials. Each bead is uniform in size, and the green-fluorescent dyes are localized within the bead, which makes the beads...

OneComp eBeads™ Compensation Beads Invitrogen™

OneComp eBeads react with antibodies of mouse, rat and hamster origin, and are immunoglobulin light chain-independent. The beads are spherical particles that can be stained with individual fluorochrome-conjugated antibodies for use as single-color compensation controls.
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