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eBioscience™ Cell Stimulation Cocktail (plus protein transport inhibitors) (500X) Invitrogen™
The Cell Stimulation Cocktail (plus protein transport inhibitors) (500X) is a cocktail of phorbol 12-myristate 13-acetate (PMA), ionomycin, brefeldin A and monensin. Treatment with PMA and ionomycin is sufficient to induce activation of many cell types to produce cytokines.
eBioscience™ Fixation/Permeabilization Concentrate Invitrogen™
The Fixation/Permeabilization Concentrate is intended to be used with antibodies to transcription factors and nuclear proteins, such as Foxp3 and Ki-67, as well as cytokines and chemokines. This must be used in combination with the Fixation/Permeabilization Diluent (cat.
eBioscience™ Permeabilization Buffer (10X) Invitrogen™
The Permeabilization Buffer (10X), in conjunction with the Intracellular (IC) Fixation Buffer (cat. 00-8222) or Foxp3 Transcription Factor Fixation/Permeabilization Concentrate and Diluent (cat. 00-5521), can be used to fix and permeabilize cells prior to performing intracellular staining of...
eBioscience™ Mouse Cytokine Positive Control Cells Invitrogen™
The Mouse Cytokine Positive Control Cells are recommended for use as positive control cells when establishing an intracellular cytokine staining protocol. Mouse splenocytes were stimulated with ConA (3 µg/mL) for two days, followed by recombinant IL-2 (20 ng/mL) and IL-4 (20 ng/mL) for three days.
123count eBeads™ Counting Beads Invitrogen™
123count eBeads are intended for use in absolute counting of cells or other particles by flow cytometry. These are 7 μm microparticles containing encapsulated dyes compatible with blue (488 nm) and violet (405 nm) excitation sources and emitting fluorescence between approximately 500 nm and 750 nm.
eBioscience™ IC Fixation Buffer Invitrogen™
The Intracellular (IC) Fixation Buffer can be used to fix cells prior to permeabilization with the Permeabilization Buffer (cat. 00-8333), when performing intracellular staining of antigens located in the cytoplasm and secretory pathway, such as cytokines, chemokines and growth factors.
AccuCheck Counting Beads Invitrogen™
AccuCheck Counting Beads are an efficient single-platform method for absolute cell counts that combines the advantages of direct flow cytometric immunophenotyping with the use of two different fluorescent beads (A and B beads).
Alignflow™ Flow Cytometry Alignment Beads for UV Lasers, 6.0 µm Invitrogen™
Molecular Probes™ AlignFlow™ flow cytometry alignment beads are reliable references for aligning, focusing, and calibrating flow cytometers.The 6.0 µm AlignFlow™ Flow Cytometry Alignment Beads for UV Lasers are fluorescent-dye infused microspheres that are optimally excitable between 350–370 nm with...
Alignflow™ Flow Cytometry Alignment Beads for Blue Lasers, 6.0 µm Invitrogen™
Molecular Probes™ AlignFlow™ flow cytometry alignment beads are reliable references for aligning, focusing, and calibrating flow cytometers.The 6.0 µm Alignflow™ Flow Cytometry Alignment Beads for Blue Lasers are fluorescent-dye infused microspheres that are excitable at 488 nm with emission in the...
Alignflow™ Flow Cytometry Alignment Beads for UV Lasers, 2.5 µm Invitrogen™
Molecular Probes™ AlignFlow™ flow cytometry alignment beads are reliable references for aligning, focusing, and calibrating flow cytometers.The 2.5 µm Alignflow™ Flow Cytometry Alignment Beads for UV Lasers are fluorescent-dye infused microspheres that are optimally excitable between 350-370 nm with...
Alignflow™ Flow Cytometry Alignment Beads for Red Lasers, 6.0 µm Invitrogen™
Molecular Probes™ AlignFlow™ flow cytometry alignment beads are reliable references for aligning, focusing, and calibrating flow cytometers.The 6.0 µm Alignflow™ Flow Cytometry Alignment Beads for Red Lasers are fluorescent-dye infused microspheres that are excitable at 630–640 nm with emission in...
Alignflow™ Flow Cytometry Alignment Beads for Blue Lasers, 2.5 µm Invitrogen™
Molecular Probes™ AlignFlow™ flow cytometry alignment beads are reliable references for aligning, focusing, and calibrating flow cytometers.The 2.5 µm Alignflow™ Flow Cytometry Alignment Beads for Blue Lasers are fluorescent-dye infused microspheres that are excitable at 488 nm with emission in the...
AbC™ Anti-Mouse Bead Kit Invitrogen™
The AbC™ Anti-Mouse Antibody Compensation Bead Kit provides a consistent, accurate, and simple-to-use technique for the setting of flow cytometry compensation when using fluorochrome-conjugated mouse antibodies with any laser in flow cytometry.
AbC™ Anti-Rat/Hamster Bead Kit Invitrogen™
The AbC™ Anti-Rat/Hamster Antibody Compensation Bead Kit provides a consistent, accurate, and simple-to-use technique for the setting of flow cytometry compensation when using fluorochrome-conjugated to rat or hamster antibodies with any laser in flow cytometry.
Cell Sorting Set-up Beads (for UV lasers) Invitrogen™
Molecular Probes™ Cell Sorting Set-up Beads are reliable standards for flow cytometry sorter instrument setup and calibration. The Cell Sorting Set-up Beads approximate the size, emission wavelength, and intensity of many biological samples, and so can be used to calibrate a flow cytometer’s cell...
