Flow Cytometry Compensation, Counting and Calibration

Reagents and kits for flow cytometer instrument calibration, sample compensation beads and kits, cell counting standards, and other reagents needed to set your instrument up.
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OneComp eBeads™ Compensation Beads Invitrogen™

OneComp eBeads react with antibodies of mouse, rat and hamster origin, and are immunoglobulin light chain-independent. The beads are spherical particles that can be stained with individual fluorochrome-conjugated antibodies for use as single-color compensation controls.

eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set Invitrogen™

The Foxp3 Transcription Factor Staining Buffer Set has been formulated and optimized for staining with antibodies to transcription factors and nuclear proteins, such as Foxp3 and Ki-67, as well as cytokines and chemokines.

eBioscience™ Cell Stimulation Cocktail (500X) Invitrogen™

The Cell Stimulation Cocktail (500X) is a cocktail of phorbol 12-myristate 13-acetate (PMA) and ionomycin. Treatment with PMA and ionomycin is sufficient to induce activation of many cell types to produce cytokines.

eBioscience™ Fixation/Permeabilization Diluent Invitrogen™

The Fixation/Permeabilization Diluent is intended to be used with antibodies to transcription factors and nuclear proteins, such as Foxp3 and Ki-67, as well as cytokines and chemokines. This must be used in combination with the Fixation/Permeabilization Concentrate (cat.

eBioscience™ Flow Cytometry Staining Buffer Invitrogen™

Our Flow Cytometry Staining Buffer is designed for use in immunofluorescent staining protocols of cells in suspension. This Flow Cytometry Staining Buffer is a buffered saline solution containing fetal bovine serum and sodium azide (0.09%) as a preservative.

eBioscience™ Foxp3 / Transcription Factor Fixation/Permeabilization Concentrate and Diluent Invitrogen™

The Foxp3 Transcription Factor Fixation/Permeabilization Concentrate and Diluent solutions for staining with antibodies to transcription factors and nuclear proteins, such as Foxp3 and Ki-67, as well as cytokines and chemokines.

UltraComp eBeads™ Compensation Beads Invitrogen™

UltraComp eBeads™ react with antibodies of mouse, rat, and hamster origin, and are immunoglobulin light chain-independent. They are designed for use in compensation with all fluorochromes excited by ultraviolet (355 nm) violet (405 nm), blue (488 nm), green (532 nm), yellow-green (561 nm), and red...

AbC™ Total Antibody Compensation Bead Kit Invitrogen™

The AbC™ Total Antibody Compensation Bead Kit provides a consistent, accurate, and simple-to-use technique for the setting of flow cytometry compensation when using fluorochrome-conjugated mouse, rat, hamster, or rabbit antibodies with any laser in flow cytometry.

Alignflow™ Flow Cytometry Alignment Beads for Red Lasers, 2.5 µm Invitrogen™

Molecular Probes® AlignFlow™ flow cytometry alignment beads are reliable references for aligning, focusing, and calibrating flow cytometers.The 2.5 µm Alignflow™ Flow Cytometry Alignment Beads for Red Lasers are fluorescent-dye infused microspheres that are excitable at 630–640 nm with emission in...

CD44 Alexa Fluor™ 488 Conjugate Kit for Live Cell Imaging Invitrogen™

Molecular Probes® human pluripotent stem cell (PSC) live imaging kits provide reagents for superior imaging of live human PSCs. This CD44 Alexa Fluor® 488 Conjugate Kit includes an optimally labeled primary antibody paired with reduced-background imaging medium (FluoroBrite™ DMEM) for the specific...

Fixation Medium (Medium A) Invitrogen™

Fixation Medium (Medium A) is a component of the FIX & PERM® Cell Permeabilization Kit. The kit is used in flow cytometry for fixing and permeabilizing cells in suspension, allowing access of antibody to intracellular targets without sacrificing scatter characteristics.

Lysing Solution, Whole Blood Lysing Solution Invitrogen™

Invitrogen Cal-Lyse™ is a lysing solution formulated to enable the lysis of erythrocytes in samples of anticoagulated human peripheral blood. Invitrogen Cal-Lyse™ lysing solution is intended to be used as an aid in the enumeration of leukocytes that have been stained with monoclonal antibodies for...

Cell Sorting Set-up Beads (for red lasers) Invitrogen™

Molecular Probes® Cell Sorting Set-up Beads are reliable standards for flow cytometry sorter instrument setup and calibration. The Cell Sorting Set-up Beads approximate the size, emission wavelength, and intensity of many biological samples, and so can be used to calibrate a flow cytometer’s cell...

Cell Sorting Set-up Beads (for blue lasers) Invitrogen™

Molecular Probes® Cell Sorting Set-up Beads are reliable standards for flow cytometry sorter instrument setup and calibration. The Cell Sorting Set-up Beads approximate the size, emission wavelength, and intensity of many biological samples, and so can be used to calibrate a flow cytometer’s cell...

CountBright™ Plus Absolute Counting Beads Invitrogen™

CountBright Plus Absolute Counting Beads are a calibrated suspension of ∼4 µm beads that encapsulate dyes that are excitable across a wide range of UV-IR wavelengths and brightly fluoresce in the emission range of 385-860 nm.
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