Cell and Tissue Imaging Calibration Tools and Standards

Imaging microscope calibration products including image intensity standards and focusing standards as well as basic sample preparation tools including fluorescence antifade reagents and kits, mounting media, tissue clearing and wash buffers, and more.
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Secure-Seal™ Hybridization Chamber Gasket, 8 chambers, 9 mm diameter, 0.8 mm deep Invitrogen™

The Secure-Seal™ hybridization sealing systems are coverslip-seal combinations that attach to microscope slides to form microwells optimized for carrying out in situ hybridization procedures. These ready-to-use hybridization gaskets have a special adhesive that bonds to glass slides in seconds,...

Secure-Seal™ Spacer, one well, 20 mm diameter, 0.12 mm deep Invitrogen™

Secure-Seal™ adhesive spacers are removable hydrophobic barriers that provide researchers with the ultimate in flexibility for isolating specimens. They may be used to isolate cells grown in culture dishes or to separate specimens on microscopy slides during staining procedures.

Secure-Seal™ Spacer, 8 wells, 9 mm diameter, 0.12 mm deep Invitrogen™

Secure-Seal™ adhesive spacers are removable hydrophobic barriers that provide researchers with the ultimate in flexibility for isolating specimens. They may be used to isolate cells grown in culture dishes or to separate specimens on microscopy slides during staining procedures.

CytoVista™ Tissue Clearing Enhancer Invitrogen™

CytoVista Tissue Clearing Enhancer helps clear fluorescently labeled tissue to enable sharp and bright 3D fluorescent imaging at any tissue depth. When used following CytoVista Tissue Clearing Reagent, this reagent can clear almost any tissue type with depths up to 10 mm.

CytoVista™ 3D Cell Culture Clearing Reagent Invitrogen™

CytoVista 3D Cell Culture Clearing Reagent clears fluorescently labeled 3D cultured cells such as organoids and spheroids. This clearing process enables sharp and bright 3D fluorescent imaging for depths up to 1000 µm.

CytoVista™ 3D Cell Culture Clearing/Staining Kit Invitrogen™

The CytoVista 3D Cell Culture Clearing/Staining Kit clears fluorescently labeled 3D cultured cells such as organoids and spheroids. This clearing process enables sharp and bright 3D fluorescent imaging for depths up to 1000 µm.

SlowFade™ Gold Antifade Mountant

SlowFade Gold Antifade Mountant is a liquid mountant applied directly to fluorescently labeled cell or tissue samples on microscope slides. It contains chemical components designed to protect fluorescent dyes from fading (photobleaching) during fluorescence microscopy experiments.

TetraSpeck™ Fluorescent Microspheres Size Kit (mounted on slide) Invitrogen™

This TetraSpeck Fluorescent Microspheres Size Kit contains one microscope slide, with six viewing regions. Each of five viewing regions contains microspheres of one size – 0.1, 0.2, 0.5, 1.0 or 4.0 µm. The remaining region contains a mixture of all five bead sizes.

Fluorescein - Reference Standard Invitrogen™

Fluorescein can be used as a reference standard for ether, ester and phosphate substrates derived from this green-fluorescent dye. See our full collection of microscope calibration reagents ›

FocalCheck™ Microspheres, 6 µm, fluorescent green ring stain/blue throughout Invitrogen™

The sharp ring stains exhibited by the 6.0 µm FocalCheck™ fluorescent green ring stained/blue throughout microspheres produce a striking visual representation of instrument misalignment or other aberrations making them ideal as reference standards for confocal laser-scanning microscopy.

FluoCells™ Prepared Slide #2 (BPAE cells with Mouse Anti-α-tubulin, BODIPY™ FL Goat Anti-Mouse IgG, Texas Red™-X Phalloidin, and DAPI) Invitrogen™

FluoCells prepared microscope slides contain multilabeled cell preparations for observation by fluorescence microscopy. FluoCells prepared slide #2 contains bovine pulmonary artery endothelial cells (BPAEC).

FluoCells™ Prepared Slide #3 (mouse kidney section with Alexa Fluor™ 488 WGA, Alexa Fluor™ 568 Phalloidin, and DAPI) Invitrogen™

The FluoCells prepared slide #3 is ideal for demonstrating the optical sectioning properties of confocal microscopes. Each slide contains a 16 µm cryostat section of mouse kidney stained with Alexa Fluor 488 wheat germ agglutinin (W-11261), Alexa Fluor 568 phalloidin (A-12380) and DAPI (D-1306).

FocalCheck™ Fluorescence Microscope Test Slide #1, for alignment, intensity, & calibration Invitrogen™

The FocalCheck™ fluorescence microscope test slide #1 is specifically designed for calibrating fluorescence microscope systems and evaluating system and filter performance. The slide contains 10 sample areas coated with FocalCheck™ ring stained microspheres ranging in size from 0.

FocalCheck™ Microspheres, 15 µm, fluorescent green/orange/dark-red ring stains Invitrogen™

The sharp ring stains exhibited by the 15 µm FocalCheck™ fluorescent green/orange/dark-red ring stained microspheres produce a striking visual representation of instrument misalignment or other aberrations making them ideal as reference standards for confocal laser-scanning microscopy.

FocalCheck™ Microspheres, 15 µm, fluorescent green ring stain/dark red throughout Invitrogen™

The sharp ring stains exhibited by the 15 µm FocalCheck™ fluorescent green ring stained/dark red throughout microspheres produce a striking visual representation of instrument misalignment or other aberrations making them ideal as reference standards for confocal laser-scanning microscopy.
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