LAMP and Other Isothermal Amplification Technologies
Isothermal amplification covers multiple technologies, including loop-mediated isothermal amplification (LAMP), that are used to amplify nucleic acids (RNA and DNA) exponentially at constant temperature, eliminating the need for thermocycler equipment. Isothermal amplification techniques are suitable for detecting various pathogens from environment, plants, human and animal samples.
Loop-mediated isothermal amplification, also known as LAMP, is a simple and cost-effective nucleic acid amplification technique that does not require expensive instruments and helps ensure rapid results and easy outcome interpretation.
Isothermal nucleic acid amplification is a technique used to amplify specific DNA or RNA sequences at a constant temperature, typically between 37–65°C, eliminating the need for the thermal cycling equipment utilized in traditional PCR ( p olymerase c hain r eaction).