Additional Cell Culture Reagents and Supplements

Additional Cell Culture Reagents and Supplements

Reagents designed to encourage the growth and expansion of specific cell lines and support analytical cellular and cell-associated analyses.
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GlutaMAX™ Supplement Gibco™

Gibco™ GlutaMAX™ Supplement is an alternative to L-glutamine, with increased stability that improves cell health. GlutaMAX™ Supplement is suitable for both adherent and suspension culture of mammalian cells, with no adaptation required.

B-27™ Supplement (50X), minus vitamin A Gibco™

A variation of the most cited neuronal cell culture supplement, B-27 Supplement Minus Vitamin A is a customized version of the original B-27 Supplement without vitamin A (retinyl acetate). B-27 Supplement Minus Vitamin A is a serum-free supplement ideal for the cultivation of neural progenitor and...

Sodium Pyruvate (100 mM) Gibco™

Sodium pyruvate is commonly added to cell culture media as a carbon source in addition to glucose. Since cells make sodium pyruvate as an intermediate metabolite in the glycolysis pathway, it is not a required supplement for all cell cultures.

Insulin-Transferrin-Selenium (ITS -G) (100X) Gibco™

Gibco™ Insulin-Transferrin-Selenium (ITS-G) is used as a basal medium supplement in order to reduce the amount of fetal bovine serum (FBS) needed to culture cells. Originally used with RPMI 1640 and Minimum Essential Medium (MEM), ITS-G solution is now routinely used with other basal media to...

2-Mercaptoethanol Gibco™

Gibco™ 2-Mercaptoethanol (also known as beta-mercaptoethanol or BME) is a potent reducing agent used in cell culture media to prevent toxic levels of oxygen radicals. 2-Mercaptoethanol is not stable in solution, so most protocols require daily supplementation.

Poly-D-Lysine Gibco™

Poly-D-Lysine is a chemically synthesized extracellular matrix used to facilitate cell adhesion to tissue culture-treated plastic and glass surfaces. Poly-D-Lysine contains polymers with a molecular weight of 50,000–150,000 Daltons, which makes this product ideal for neuronal culture applications.

Anti-Clumping Agent Gibco™

GIBCO™ Anti-Clumping Agent is a concentrated liquid reagent that reduces cell clumping to assist in attaining higher viable cell densities in suspension culture. Anti-Clumping Agent is a gentle, animal origin-free, chemically defined formulation that contains no proteins, enzymes, hydrolysates, or...

Chemically Defined Lipid Concentrate Gibco™

Insect cell culture tested. Chemically Defined Lipid Concentrate is a concentrated lipid emulsion designed to reduce or replace fetal bovine serum in cell culture media for a wide variety of applications, including growth and maintenance of CHO, hybridoma, and insect cells in culture; monoclonal...

MEM Amino Acids Solution (50X) Gibco™

Gibco™ MEM Amino Acids Solution is used as a growth supplement for cell culture medium, to increase cell growth and viability. Gibco™ MEM Amino Acids Solution is formulated to contain 50X the essential amino acids (except L-glutamine) found in the standard Minimum Essential Medium (MEM).

eBioscience™ Lipopolysaccharide (LPS) Solution (500X) Invitrogen™

The Lipoploysaccharide (LPS) Solution (500X) is a ready to use solution of LPS from Escherichia coli 026:B6 in aqueous solution. LPS is a major component of the cell wall of gram negative bacteria. It is highly immunogenic and strongly activates immune cells bearing the CD14/TLR4/MD2 receptor...

Primary Hepatocyte Maintenance Supplements Gibco™

Hepatocyte Maintenance Supplement Packs allow you to supplement up to 500 ml of Williams Medium E without phenol red (or suitable alternative basal medium) for the purpose of incubating hepatocytes in suspension or plated cultures.

HT Supplement (100X) Gibco™

Gibco™ HT Supplement is a liquid mixture of sodium hypoxanthine (10 mM) and thymidine (1.6 mM). HT-supplemented medium is suitable for post-selection rescue to overcome the effects of residual intracellular aminopterin.

Bovine Albumin Fraction V (7.5% solution) Gibco™

7.5 g/100 ml Prepared with Bovine Serum Albumin Fraction V in phosphate-buffered saline.

HEPES (Ultra Pure) Gibco™

Gibco™ HEPES (N-2-Hydroxyethylpiperazine-N-2-Ethane Sulfonic Acid) is a zwitterionic organic chemical buffering agent commonly used in cell culture media. The addition of 10 – 25 mM HEPES provides extra buffering capacity when cell culture requires extended periods of manipulation outside of a CO2...

Dexamethasone, 10 mM in DMSO Gibco™

This solution contains 10 mM dexamethasone in DMSO. Dexamethasone is an anti-inflammatory glucocorticoid used as a supplement in hepatocyte cell culture. This product is available as a separate component for the purposes of supplementing William's E Medium, no phenol red (Cat. No.

CTS™ (Cell Therapy Systems) N-2 Supplement Gibco™

CTS N-2 Supplement is a serum-free supplement for the growth and expression of neuroblastomas as well as post-mitotic neurons in primary cultures from both the peripheral nervous system and the central nervous system.

Large Vessel Endothelial Supplement (LVES) Gibco™

Gibco™ Large Vessel Endothelial Supplement (LVES) is a 50X, complete, ready-to-use media supplement optimized for use with Gibco™ Medium 200 and Medium 200PRF for culture of primary human umbilical vein endothelial cells (HUVEC), human aortic endothelial cells (HAEC), and human pulmonary artery...

CHO CD EfficientFeed™ A Nutrient Supplement Gibco™

Gibco™ CHO CD EfficientFeed™ A liquid nutrient supplement is an animal origin-free, chemically defined complete feed formulation that provides up to 2-5 fold improvement in titers used in fed batch versus batch culture.

Trypan Blue Solution, 0.4% Gibco™

Trypan Blue Solution, 0.4%, is routinely used as a cell stain to assess cell viability using the dye exclusion test. This test is often performed while counting cells with the hemocytometer during routine subculturing, but can be performed any time cell viability needs to be determined quickly and...

AlbuMAX™ II Lipid-Rich BSA Gibco™

Lipid-rich bovine serum albumin for cell culture. It is chromatographically purified and has an IgG content <=1.0%. Origin: New Zealand

AlbuMAX™ I Lipid-Rich BSA Gibco™

Lipid-rich bovine serum albumin for cell culture (1). It is chromatographically purified and has an IgG content <= 0.1%. Origin: New Zealand

Bovine Transferrin, HOLO form (custom product) Gibco™

This is a GIBCO Custom Product. Please complete and submit the Custom Media Inquiry Form and a Customer Service Representative will contact you regarding pricing and availability.

MEM Vitamin Solution (100X) Gibco™

Gibco™ MEM Vitamin Solution is used as a growth supplement for cell culture medium, to increase cell growth and viability. Gibco™ MEM Vitamin Solution is formulated to contain 100x the vitamins found in the standard Minimum Essential Medium (MEM). The complete formulation is available.

B-27™ Supplement (50X), minus antioxidants Gibco™

A variation of the most cited neuronal cell culture supplement, B-27 Supplement Minus Antioxidants (AO) is a customized version of the original B-27 Supplement without five antioxidants (vitamin E, vitamin E acetate, superoxide dismutase, catalase, and glutathione).

MEM Non-Essential Amino Acids Solution (100X) Gibco™

Gibco™ MEM Non-Essential Amino Acids are used as a supplement for cell culture medium, to increase cell growth and viability. Gibco® MEM Non-Essential Amino Acids contains the same non-essential amino acids found in the standard Minimum Essential Medium (MEM) at a strength of 100X.

Tryptose Phosphate Broth

Gibco™ Tryptose Phosphate Broth is a cell culture supplement for BHK-21 cells used for vaccine production. Tryptose Phosphate Broth contains tryptose (an enzymatic hydrolysis of protein), dextrose, sodium chloride and disodium phosphate. Product Use For Research Use Only.

Agarose (4%) Gibco™

Gibco™ 4% Agarose is an autoclaved, high purity, low melting point gel designed for plaque assays and the purification of baculovirus. The 4% Agarose Gel melts at 65 – 70°C and remains fluid at 37°C. When diluted with concentrated insect cell culture medium, Gibco™ 4% Agarose produces a gel firm...

PBS Tablets Gibco™

Phosphate-buffered saline (PBS) is a balanced salt formulation used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.

G-5 Supplement (100X) Gibco™

G-5 Supplement is a chemically defined, serum-free supplement based on Bottenstein’s G-5 formulation. It is recommended for growth and expression of glial cells (normal and tumor) of astrocytic phenotype (astrocytes).

B-27™ Supplement (50X), serum free Gibco™

The most cited neuronal cell culture supplement, B-27 Supplement is an optimized serum-free supplement used to support the low- or high-density growth and short- or long-term viability of embryonic, post-natal, and adult hippocampal and other CNS neurons.

KaryoMAX™ Colcemid™ Solution in HBSS Gibco™

Gibco™ KaryoMAX™ Colcemid™ Solution in HBSS is a 10 µg/mL N-desacetyl-N-methylocolchicine (Colcemid™) solution made up in Hanks' Balanced Salt Solution (HBSS). Colcemid™ prevents spindle formation during mitosis, arresting cells in metaphase so that the chromosomes can be separated for cytogenetic...

Glucose, powder Gibco™

Gibco™ Glucose powder is a supplement for cell culture media. Most cell culture media use D-glucose (dextrose) as the cells' main source of metabolic energy and reduced carbon. Gibco™ Glucose powder should be made as a sterile-filtered 20% stock solution (or similar) in distilled water before use.

Dopaminergic Neuron Maturation Supplement Gibco™

Dopaminergic Neuron Maturation Supplement is a component of the Gibco™ PSC Dopaminergic Neuron Differentiation Kit that is also offered separately. It enables maturation of excess floor plate progenitor cells to midbrain dopaminergic neurons.

B-27™ Supplement, XenoFree, minus vitamin A Gibco™

B-27 Supplement XenoFree Minus Vitamin A is based on the classic B-27 Supplement formulation and contains no animal-derived components at the primary component level. The absence of vitamin A (retinyl acetate) makes this formulation ideal for the cultivation of neural progenitor and stem cells,...

B-27™ Plus Supplement (50X) Gibco™

B-27 Plus Supplement is the next-generation of the most cited neuronal cell culture product, B-27 Supplement. It is a serum-free supplement that provides the highest rate of in vitro survival of primary rodent and human stem cell-derived neurons when used with Neurobasal Plus Medium.

Insulin, human recombinant, zinc solution Gibco™

4 mg/ml Potency: (dry basis) >= 27 USP units/mg. Suggested range for supplementation: 2 to 20 µg/ml.

AmnioMAX™ C-100 Supplement Gibco™

Gibco™ AmnioMAX™ C-100 Supplement is part of a kit used to prepare a fully-supplemented Gibco™ AmnioMAX™ C-100 Complete Medium developed for the short term culture of human amniotic fluid cells for cytogenetic studies and in vitro diagnostic procedures.

Phytohemagglutinin, M form (PHA-M) Gibco™

Gibco™ Phytohemagglutinin, M form (PHA-M) is a solution made from a crude extract of the red kidney bean Phaseolus vulgaris. This extract is capable of inducing blastogenesis in vitro in various mammalian mononuclear cells when used at a concentration of 1–2% v/v.

Supplement S7 Gibco™

Supplement S7 is a sterile, concentrated (100X), ionically balanced solution intended for use as one component in a complete culture environment for human epidermal keratinocytes. Supplement S7 is chemically defined and animal origin-free.

L-Glutamine (200 mM) Gibco™

L-Glutamine is an amino acid that is required for cell culture. L-Glutamine participates in the formation of purine and pyrimidine nucleotides, amino sugars, glutathione, L-glutamate, and other amino acids, as well as in protein synthesis and glucose production.

Pluronic™ F-68 Non-ionic Surfactant (100X) Gibco™

Gibco™ Pluronic™ F-68 is a non-ionic surfactant used to control shear forces in suspension cultures. Pluronic™ F-68 can also be used to reduce foaming in stirred cultures and reduce cell attachment to glass.

2-Mercaptoethanol (50 mM) Gibco™

Gibco™ 2-Mercaptoethanol (also known as beta-mercaptoethanol or BME) is a potent reducing agent used in cell culture medium to prevent toxic levels of oxygen radicals. Mercaptoethanol is not stable in solution so most protocols require daily supplementation.

HAT Supplement (50X)

Gibco™ HAT Supplement is a liquid mixture of sodium hypoxanthine (5 mM), aminopterin (20 µM) and thymidine (0.8 mM). HAT-supplemented medium is suitable for post-fusion selection against unfused or self-fused HGPRT–myeloma cells.

EfficientFeed™ C+ 2X Supplement Gibco™

Gibco EfficientFeed C+ 2X Supplement is a single-part liquid supplement for fed batch addition to the culture of multiple cell lines to assist with process development and maximization of bioreactor utilization.

rhLaminin-521 Gibco™

Recombinant human Laminin 521 (rhLaminin-521) is a recombinant human protein that provides a defined surface for feeder-free culture of human pluripotent stem cells (PSCs). When used with Essential 8™ Medium, rhLaminin-521 has been proven to promote cellular survival under stressful conditions in...

Glucose Solution Gibco™

Gibco™ Glucose Solution contains D-glucose at a concentration of 200 g/L and is used as a serum-free medium supplement suitable for cell culture. Glucose Solution can be added to a variety of Gibco™ cell culture media including but not limited to Dulbecco’s Modified Eagle Medium (DMEM), RPMI 1640,...

B-27™ Supplement, minus insulin Gibco™

A variation of the most cited neuronal cell culture supplement, B-27 Supplement Minus Insulin is a customized version of the original B-27 Supplement without insulin. B-27 Supplement Minus Insulin is a serum-free supplement designed to support growth of hippocampal and other CNS neurons in...

Primary Hepatocyte Thawing and Plating Supplements Gibco™

Hepatocyte Thawing and Plating Supplement Packs allow you to supplement up to 500 ml of Williams Medium E without phenol red (or a suitable alternative basal medium) for the purpose of plating fresh or cryopreserved hepatocytes.

Hepatocyte Thaw Medium Gibco™

Gibco™ Hepatocyte Thaw Medium is a ready-to-use medium specifically designed for use with thawed cryopreserved hepatocytes to enhance their viability and overall quality. This medium is provided in a 50 mL conical tube for ease of use.

HepaRG™ Induction Medium Supplement (5X) Gibco™

HepaRG™ Induction Medium Supplement (5x) is a specialized media supplement for use in culturing HepaRG™ cells. HepaRG™ Induction Medium Supplement (5x) is specifically used for induction of metabolic activity in HepaRG™ cells.

HepaRG™ Induction Medium Supplement Gibco™

HepaRG™ Induction Medium Supplement is a specialized media supplement for use in culturing HepaRG™ cells. HepaRG™ Induction Medium Supplement is specifically used for induction of metabolic activity in HepaRG™ cells.

HepaRG™ Serum-free Induction Medium Supplement Gibco™

HepaRG™ Serum-free Induction Medium Supplement is a specialized media supplement for use in culturing HepaRG™ cells. HepaRG™ Serum-free Induction Medium Supplement is specifically used for induction of metabolic activity in HepaRG™ cells in a serum free environment.

Smooth Muscle Differentiation Supplement (SMDS) Gibco™

A sterile, concentrated (100X) solution intended for use with Medium 231 to enhance differentiation of human smooth muscle cells. Each 5 ml bottle of SMDS contains the correct amount of differentiation supplement for a 500 ml bottle of Medium 231.

HepaRG™ Maintenance/Metabolism Medium Supplement Gibco™

HepaRG™ Maintenance/Metabolism Medium Supplement is a specialized media supplement for use in culturing HepaRG™ cells. HepaRG™ Maintenance/Metabolism Medium Supplement is specifically used for multiple day growth of HepaRG™ cells.

Cryopreserved Hepatocyte Plating Medium (CHPM) Gibco™

Cryopreserved Hepatocyte Plating Medium (CHPM) used for plating Gibco™ cryopreserved human hepatocytes for optimal attachment and monolayer formation. For research use only. Not intended for human or animal therapeutic or diagnostic use.

Cryopreserved Hepatocyte Recovery Medium (CHRM) Gibco™

Cryopreserved Hepatocyte Recovery Medium (CHRM) is used for thawing Gibco™ cryopreserved human hepatocytes for optimal cell recovery and viability. CHRM is sold in 50 ml conical tubes for ease of use. To ensure best results when using Gibco™ cryopreserved human hepatocytes, use a one step...

HepaRG™ Thaw, Plate & General Purpose Medium Supplement (5X) Gibco™

HepaRG™ Thaw, Plate, & General Purpose Medium Supplement (5x) is a specialized media supplement for use in culturing HepaRG™ cells. HepaRG™ Thaw, Plate, & General Purpose Medium Supplement (5x) is specifically used for the initial thawing and propagation of cryopreserved HepaRG™ cells.

HepaRG™ Maintenance/Metabolism Medium Supplement (5X) Gibco™

HepaRG™ Maintenance/Metabolism Medium Supplement (5x) is a specialized media supplement for use in culturing HepaRG™ cells. HepaRG™ Maintenance/Metabolism Medium Supplement (5x) is specifically used for multiple day growth of HepaRG™ cells.
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