DMEM/F-12 combines Dulbecco's modified Eagle medium (DMEM) and Ham's F-12 nutrient mixture to create a versatile cell culture medium that supports the growth and maintenance of various cell types in laboratory settings. This blend provides essential nutrients like insulin, transferrin, and growth factors, promoting cell proliferation and survival. Widely used for culturing primary mammalian cells and different cell lines, DMEM/F-12 is valued for its ability to support a broad range of cell types, making it a valuable tool in cellular research and biotechnological applications.
Cell culture medium is vital for providing the necessary components to support cell growth and function. Components in cell culture media supply your culture environment with nutrients and growth factors for the cells to behave as expected.
Media supplements are often a vital component of successful cell culture experiments. There are many advantages of using cell culture supplements including customizing the growth conditions of your cells, improving cell viability and growth, and keeping cells healthier longer.