Shop All Magnetic Beads for Cell Isolation & Expansion

Dynabeads™ Untouched™ Human CD8 T Cells Kit (Invitrogen™)

The Dynabeads® Untouched™ Human CD8 T Cells Kit is used to isolate pure and viable untouched CD8+ T cells from PBMC by negative isolation. The kit depletes CD4+ T cells, B cells, NK cells, monocytes, platelets, dendritic cells, granulocytes, and erythrocytes. The negatively isolated human CD8+ T-cells are left in the sample and have not been in contact with the Dynabeads®. The bead- and antibody-free CD8+ T cells are truly untouched, and in perfect shape for any functional assay or application.

Advantages of the Dynabeads® Untouched™ Human CD8 T Cells Kit:

• High purity, recovery, and viability of human untouched CD8+ T cells
• Easy to use and to scale up
• No columns required

Magnetic bead-based Separation offers easy handling
An antibody mix towards the non-CD8 cells is added to the sample and allowed to bind to the cells. Dynabeads® are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched human CD8+ T cells can be directly analyzed in a flow cytometer and used in any application. For depletion or positive isolation of CD8+ cells, select from our range of Human CD8+ T Cell Isolation Products.

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MagniSort™ Mouse CD4 Naïve T cell Enrichment Kit (Invitrogen™)

The MagniSort™ Mouse CD4 Naïve T cell Enrichment Kit is designed for the magnetic separation of CD4 naïve T cells by negative selection. It has been optimized for the isolation of mouse CD4 naïve T cells from spleens or lymph nodes utilizing a biotinylated antibody cocktail and streptavidin-coated magnetic beads. Undesired cells are bound by antibody and then magnetic beads that, when placed in a magnetic field, leave CD4 naïve T cells untouched and free in solution.

The MagniSort™ Mouse CD4 Naïve T cell Enrichment Antibody Cocktail contains the following antibodies:
Anti-Mouse CD8 Biotin
Anti-Mouse CD11b Biotin
Anti-Mouse CD19 Biotin
Anti-Mouse CD24 Biotin
Anti-Mouse CD44 Biotin
Anti-Mouse CD45R (B220) Biotin
Anti-Mouse CD49b (Integrin alpha 2) Biotin
Anti-Mouse Ly-6G (Gr-1) Biotin
Anti-Mouse γδ TCR Biotin
Anti-Mouse TER-119 Biotin

Reported Application
Magnetic Cell Separation

Dynabeads™ Untouched™ Human NK Cells Kit (Invitrogen™)

Use this kit to isolate pure and viable untouched NK cells from PBMC by negative isolation. The kit depletes T cells, B cells, monocytes, platelets, dendritic cells, granulocytes and erythrocytes. The negatively isolated human NK cells are left in the sample and have not been in contact with the Dynabeads. The bead and antibody-free NK cells are truly untouched, and in perfect shape for any functional assay and application.

• High purity, recovery and viability of human untouched NK cells
• Easy to use and to scale up
• No columns required

Methodology:
An antibody mix towards the non-NK cells is added to the sample and allowed to bind to the cells. Dynabeads® are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched human NK cells can be directly analyzed in a flow cytometer and used in any application.

Starting Sample:
PBMC

Dynabeads™ Untouched™ Mouse CD8 Cells Kit (Invitrogen™)

Dynabeads® Untouched™ Mouse CD8 Cells supplies both 4.5 µm superparamagnetic Dynabeads® (with a polyclonal sheep anti-rat IgG antibody covalently bound to the bead surface) and a monoclonal Antibody Mix, together designed for the depletion of CD8-negative leucocytes from mouse spleen or lymph node single-cell suspensions. Following the depletion procedure, the negatively isolated, untouched target mouse CD8+ cells are pure, viable, and ready to be used in any downstream application.

• Rapid isolation of untouched mouse CD8+ T cells—no columns required
• Pure and viable mouse CD8+ T cells are ready for any downstream application

Excellent yield, purity, and viability of recovered cells
The Antibody Mix contains a cocktail of rat IgGs that binds mouse CD4+ T cells, B cells, NK cells, monocytes/macrophages, dendritic cells, erythrocytes, and granulocytes. The Antibody Mix is first added to the sample in a tube to bind the unwanted CD8-negative cells. The cells are then washed to remove excess antibodies. Mouse Depletion Dynabeads® (coated with a rat IgG antibody) are added to bind the antibody-labeled, CD8-negative cells. After a short incubation, the bead-bound cells are then separated from unbound cells in 1–2 minutes using a magnet. The purified target mouse CD8+ cells in the supernatant can be transferred to a new tube for use in any downstream application.

This fast and gentle isolation method does not require the use of columns, and helps ensure high purity, recovery and viability of the untouched CD8+ cells. The protocol can be scaled up according to your sample size.

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Dynabeads™ CD8 Positive Isolation Kit (Invitrogen™)

The Dynabeads® CD8 Positive Isolation Kit supplies superparamagnetic beads that enable gentle isolation of pure CD8+ T cells from whole blood, bone marrow, buffy coat, mononuclear cells (MNC), or tissue digests. The cells are released from the beads using the DETACHaBEAD® reagent to obtain bead- and antibody-free cells. Advantages of the Dynabeads® CD8 Positive Isolation Kit:

• Gentle isolation of CD8+ T cells from any sample—no columns required
• Bead- and antibody-free positive isolation of CD8+ cells for any downstream assay

About Dynabeads® CD8 Positive Isolation Kit
The Dynabeads® CD8 Positive Isolation Kit contains uniform, superparamagnetic beads (4.5 µm diameter) covalently coated with a monoclonal antibody specific for the CD8 membrane antigen predominantly expressed on the cytotoxic subset of human T cells. The kit also includes a DETACHaBEAD® reagent that releases the Dynabeads® from the purified CD8+ cells. This gently magnetic isolation process gives highly viable bead- and antibody-free cells to be used in any downstream assay.

Magnetic bead–based separation offers easy handling
Dynabeads® are mixed with the sample in a tube where they bind to the target cells during a short incubation, after which the bead-bound cells are separated from unbound cells using a magnet. The positively isolated cells are then washed, and DETACHaBEAD® reagent is added to gently release the cells from the beads. This magnetic bead-based positive isolation method gives both bead- and antibody-free cells. The isolated CD8+ cells are phenotypically unaltered and suitable for any downstream application. This fast and gentle isolation method does not require the use of columns, and helps ensure high purity and viability of the isolated CD8+ cells. For depletion or isolation of untouched CD8+ cells, select from our range of Human CD8+ T Cell Isolation Products.

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MagniSort™ Mouse CD8 Naïve T cell Enrichment Kit (Invitrogen™)

The MagniSort™ Mouse CD8 Naïve T cell Enrichment Kit is designed for the magnetic separation of CD8 naïve T cells by negative selection. It has been optimized for the isolation of mouse CD8 naïve T cells from spleens or lymphnodes utilizing a biotinylated antibody cocktail and streptavidin-coated magnetic beads. Undesired cells are bound by antibody and then magnetic beads that, when placed in a magnetic field, leave CD8 naïve T cells untouched and free in solution.

The MagniSort™ Mouse CD8 Naïve T cell Enrichment Antibody Cocktail contains the following antibodies:
Anti-Mouse CD4 Biotin
Anti-Mouse CD11b Biotin
Anti-Mouse CD19 Biotin
Anti-Mouse CD24 Biotin
Anti-Mouse CD44 Biotin
Anti-Mouse CD45R (B220) Biotin
Anti-Mouse CD49b (Integrin alpha 2) Biotin
Anti-Mouse Ly-6G (Gr-1) Biotin
Anti-Mouse γδ TCR Biotin
Anti-Mouse TER-119 Biotin

Reported Application
Magnetic Cell Separation

MagniSort™ Human CD8 Memory T cell Enrichment Kit (Invitrogen™)

The MagniSort™ Human CD8 Memory T cell Enrichment Kit is designed for the magnetic separation of CD8 memory T cells by negative selection. It has been optimized for the isolation of human CD8 memory T cells from normal human peripheral blood mononuclear cells utilizing a biotinylated antibody cocktail and streptavidin-coated magnetic beads. Undesired cells are bound by antibody and then magnetic beads that, when placed in a magnetic field, leave CD8 memory T cells untouched and free in solution.

The MagniSort™ Human CD8 Memory T cell Enrichment Antibody Cocktail contains the following antibodies:
Anti-Human CD4 Biotin
Anti-Human CD11b Biotin
Anti-Human CD14 Biotin
Anti-Human CD16 Biotin
Anti-Human CD19 Biotin
Anti-Human CD20 Biotin
Anti-Human CD36 Biotin
Anti-Human CD45RA Biotin
Anti-Human CD56 Biotin
Anti-Human CD123 Biotin
Anti-Human CD235a Biotin
Anti-Human γδ TCR Biotin

Reported Application
Magnetic Cell Separation

MagniSort™ Mouse B cell Enrichment Kit (Invitrogen™)

The MagniSort™ Mouse B cell Enrichment Kit is designed for the magnetic separation of mouse B cells by negative selection. It has been optimized for the isolation of mouse B cells from spleens or lymph nodes utilizing a biotinylated antibody cocktail and streptavidin-coated magnetic beads. Undesired cells are bound by antibody and then magnetic beads that, when placed in a magnetic field, leave B cells untouched and free in solution.

The MagniSort™ Mouse B cell Enrichment Antibody Cocktail contains the following antibodies:
Anti-Mouse CD3 Biotin
Anti-Mouse CD4 Biotin
Anti-Mouse CD8 Biotin
Anti-Mouse CD11b Biotin
Anti-Mouse CD49b Biotin
Anti-Mouse Ly-6G (Gr-1) Biotin
Anti-Mouse TER-119 Biotin

Reported Application
Magnetic Cell Separation

MagniSort™ Human CD8 Naïve T cell Enrichment Kit (Invitrogen™)

The MagniSort™ Human CD8 Naïve T cell Enrichment Kit is designed for the magnetic separation of CD8 naïve T cells by negative selection. It has been optimized for the isolation of human CD8 naïve T cells from normal human peripheral blood mononuclear cells utilizing a biotinylated antibody cocktail and streptavidin-coated magnetic beads. Undesired cells are bound by antibody and then magnetic beads that, when placed in a magnetic field, leave CD8 naïve T cells untouched and free in solution.

The MagniSort™ Human CD8 Naïve T cell Enrichment Antibody Cocktail contains the following antibodies:
Anti-Human CD4 Biotin
Anti-Human CD11b Biotin
Anti-Human CD14 Biotin
Anti-Human CD16 Biotin
Anti-Human CD19 Biotin
Anti-Human CD20 Biotin
Anti-Human CD36 Biotin
Anti-Human CD45RO Biotin
Anti-Human CD56 Biotin
Anti-Human CD123 Biotin
Anti-Human CD235a Biotin
Anti-Human γδ TCR Biotin

Reported Application
Magnetic Cell Separation

Dynabeads™ CD4 (Invitrogen™)

Dynabeads® CD4 are superparamagnetic beads coupled with an anti-human CD4 antibody that enable easy isolation or deplettion of human CD4+ T cells directly from any sample, including whole blood, bone marrow, buffy coat, mononuclear cells (MNC), and tissue digests. Advantages of Dynabeads® CD4:

• Fast and efficient isolation of CD4+ T cells from any sample—no columns required
• High purity, yield, and viability of positively isolated CD4+ T cells for molecular downstream assays
• Fast and efficient depletion of CD4+ T cells from any sample

About Dynabeads® CD4
Dynabeads® CD4 are uniform, superparamagnetic beads (4.5 µm diameter) coated with a primary monoclonal antibody specific for the CD4 membrane antigen mainly expressed on human T helper cells. Because of the bead size, Dynabeads® CD4 can easily and efficiently isolate or deplete cells from viscous samples such as whole blood and bone marrow in about 30 minutes. Dynabeads® CD4 will also afford excellent recovery of high-purity, viable cells for use in downstream molecular studies; for example, those in which cells are to be lysed while still attached to beads, and nucleic acids or proteins further purified. Note that intact cells will not be released from these beads, so if you want to isolate CD4+ cells for cell-based applications, or you need to check your samples with flow cytometry, check out our total human CD4 product offerings.

Magnetic bead–based separation offers easy handling
Dynabeads® CD4 is added to the sample under continuous mixing to optimize the binding of the Dynabeads® to the target cells. By placing the sample on a magnet, it separates the bead-bound target cells from the rest of the sample in just 1–2 minutes. For depletion, remove the supernatant to a new tube for further studies and discard the bead-bound cells. For positive isolation for molecular studies, remove the supernatant and wash the bead-bound cells 2–3 times in buffer to get optimal purity. The cells can be lysed while still attached to the beads and the supernatant transferred to a new tube for downstream molecular analysis. Starting samples can be whole blood, buffy coat, bone marrow, PBMC, or tissue digests.

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MagniSort™ Mouse NK cell Enrichment Kit (Invitrogen™)

The MagniSort™ Mouse NK cell Enrichment Kit is designed for the magnetic separation of mouse NK cells by negative selection. This kit will not isolate NKT cells. It has been optimized for the isolation of mouse NK cells from spleens utilizing a biotinylated antibody cocktail and streptavidin-coated magnetic beads. Undesired cells are bound by antibody and then magnetic beads that, when placed in a magnetic field, leave NK cells untouched and free in solution.

The MagniSort™ Mouse NK cell Enrichment Antibody Cocktail contains the following antibodies:
Anti-Mouse CD3 Biotin
Anti-Mouse CD4 Biotin
Anti-Mouse CD8 Biotin
Anti-Mouse CD19 Biotin
Anti-Mouse MHC Class II (I-A/I-E) Biotin
Anti-Mouse F4/80 Antigen Biotin
Anti-Mouse Ly-6G (Gr-1) Biotin
Anti-Mouse TER-119 Biotin

Reported Application
Magnetic Cell Separation

MagniSort™ Human NK cell Enrichment Kit (Invitrogen™)

The MagniSort™ Human NK cell Enrichment Kit is designed for the magnetic separation of NK cells by negative selection. It has been optimized for the isolation of human NK cells from normal human peripheral blood mononuclear cells utilizing a biotinylated antibody cocktail and streptavidin-coated magnetic beads. Undesired cells, including NKT cells, are bound by antibody and then magnetic beads that, when placed in a magnetic field, leave NK cells untouched and free in solution.

The MagniSort™ Human NK cell Enrichment Antibody Cocktail contains the following antibodies:
Anti-Human CD3 Biotin
Anti-Human CD4 Biotin
Anti-Human CD14 Biotin
Anti-Human CD19 Biotin
Anti-Human CD20 Biotin
Anti-Human CD66b Biotin
Anti-Human CD123 Biotin
Anti-Human CD235a Biotin
Anti-Human HLA-DR Biotin

Reported Application
Magnetic Cell Separation

Dynabeads™ Epithelial Enrich (Invitrogen™)

Enrich epithelial tumor cells directly from whole blood, bone marrow, or PBMC suspensions. Enriched tumor cells are lysed for mRNA isolation and RT-PCR amplification. The sensitivity of combined enrichment and RT-PCR detection can be as high as 1 tumor cell in 1 ml blood.

• Depletion of epithelial tumor cells from whole blood/bone marrow or PBMC.
• Positive isolation of epithelial tumor cells for downstream molecular analysis.
• No columns required

Starting samples:
Whole blood, bone marrow, PBMC, buffy coat.

Number of cells processed:
200 ml whole blood or ~4 × 109 PBMC.

Dynabeads™ CD3 (Invitrogen™)

Dynabeads® CD3 are superparamagnetic beads coupled with an anti-human CD3 antibody that enable easy isolation or depletion of human CD3+ T cells directly from any sample, including whole blood, bone marrow, buffy coat, mononuclear cells (MNC), and tissue digests. Advantages of Dynabeads® CD3:

• Fast and efficient isolation of CD3+ T cells from any sample—no columns required
• High purity, yield, and viability of positively isolated CD3+ T cells for molecular downstream assays
• Fast and efficient depletion of CD3+ T cells from any sample

About Dynabeads® CD3
Dynabeads® CD3 are uniform, superparamagnetic beads (4.5 µm diameter) coated with a primary monoclonal antibody specific for the CD3 membrane antigen mainly expressed mainly on human mature T cells. Because of the bead size, Dynabeads® CD3 can easily and efficiently isolate or deplete cells from viscous samples such as whole blood and bone marrow in about 30 minutes. Dynabeads® CD3 will also afford excellent recovery of high-purity, viable cells for use in downstream molecular studies; for example, those in which cells are to be lysed while still attached to beads, and nucleic acids or proteins further purified. Note that intact cells will not be released from these beads, so if you want to isolate CD3+ cells for cell-based applications, or you need to check your samples with flow cytometry, check out our total human T cell product offerings.

Magnetic bead–based separation offers easy handling
Dynabeads® CD3 is added to the sample under continuous mixing to optimize the binding of the Dynabeads® to the target cells. By placing the sample on a magnet, it separates the bead-bound target cells from the rest of the sample in just 1–2 minutes. For depletion, remove the supernatant to a new tube for further studies and discard the bead-bound cells. For positive isolation for molecular studies, remove the supernatant and wash the bead-bound cells 2–3 times in buffer to get optimal purity. The cells can be lysed while still attached to the beads and the supernatant transferred to a new tube for downstream molecular analysis. Starting samples can be whole blood, buffy coat, bone marrow, PBMC, or tissue digests.

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MagniSort™ Mouse Hematopoietic Lineage Depletion Kit (Invitrogen™)

The MagniSort™ Mouse Hematopoietic Lineage Depletion Kit is designed for the magnetic depletion of mouse hematopoietic cells with committed lineages from mouse bone marrow. It utilizes a cocktail of antibody specific for hematopoietic lineages and streptavidin-coated magnetic beads. Lineage committed cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the lineage negative cells can be separated from lineage committed cells by decanting.

The MagniSort™ Mouse Hematopoietic Lineage Depletion Antibody Cocktail contains the following antibodies:
Anti-Mouse CD2 Biotin
Anti-Mouse CD3 Biotin
Anti-Mouse CD5 Biotin
Anti-Mouse CD11b Biotin
Anti-Mouse CD19 Biotin
Anti-Mouse CD45R Biotin
Anti-Mouse Ly-6G (Gr-1) Biotin
Anti-Mouse TER-119 Biotin

After depletion, the enrichment of multi-potent progenitor cells can be verified by staining with Anti-Mouse CD34 (clone RAM34), and the enrichment of CD34- hematopoietic stem cells can be verified by additional staining with Anti-Mouse CD150 (clone mShad150) and Anti-Mouse CD48 (clone HM48-1).

Reported Application
Magnetic Cell Separation