Shop All Magnetic Beads for Cell Isolation & Expansion

Dynabeads™ CD19 Pan B Invitrogen™

Dynabeads® CD19 Pan B are superparamagnetic beads covalently coupled with an anti-human CD19 antibody that enable easy isolation or depletion of human CD19+ B cells directly from whole blood, bone marrow, buffy coat, mononuclear cells (MNC), and tissue digests. Advantages of Dynabeads® CD19 Pan B:

• Fast and efficient isolation of CD19+ B cells from any sample—no columns required
• High purity, yield, and viability of positively isolated CD19+ cells for molecular downstream assays or efficient depletion of CD19+ B cells
• Fast and efficient depletion of CD19+ T cells from any sample

About Dynabeads® CD19 Pan B
Dynabeads® CD19 pan B are uniform (4.5 µm diameter) superparamagnetic beads coated with a primary monoclonal antibody specific for the CD19 membrane antigen mainly expressed on human B cells. Because of the bead size, Dynabeads® CD19 pan B can easily and efficiently isolate or deplete cells from viscous samples such as whole blood and bone marrow in about 30 minutes. Dynabeads® CD19 pan B will also afford excellent recovery of high-purity, viable cells for use in downstream molecular studies; for example, those in which cells are to be lysed while still attached to beads, and nucleic acids or proteins further purified. Note that intact cells will not be released from these beads, so if you want to isolate CD19+ cells for cell-based applications, or you need to check your samples with flow cytometry, you can combine with DETACHaBEAD® CD19 (purchased separately to get bead- and antibody-free cells) or use Dynabeads® Untouched™ Human B Cells to obtain untouched human B cells.

Magnetic bead-based separation offers easy handling
Dynabeads® CD19 Pan B is added to the sample under continuous mixing to optimize the binding of the Dynabeads® to the target cells. By placing the sample on a magnet, it separates the bead-bound target cells from the rest of the sample in just 1–2 minutes. For depletion, remove the supernatant to a new tube for further studies and discard the bead-bound cells. For positive isolation for molecular studies, remove the supernatant and wash the bead-bound cells 2–3 times in buffer to get optimal purity. The cells can be lysed while still attached to the beads and the supernatant transferred to a new tube for downstream molecular analysis. Starting samples can be whole blood, buffy coat, bone marrow, PBMC, or tissue digests.

Learn more about Dynabeads® products
• Find Dynabeads® products for a whole range of applications.
• Find magnets for Dynabeads® separations.

Dynabeads™ CD4 Invitrogen™

Dynabeads® CD4 are superparamagnetic beads coupled with an anti-human CD4 antibody that enable easy isolation or deplettion of human CD4+ T cells directly from any sample, including whole blood, bone marrow, buffy coat, mononuclear cells (MNC), and tissue digests. Advantages of Dynabeads® CD4:

• Fast and efficient isolation of CD4+ T cells from any sample—no columns required
• High purity, yield, and viability of positively isolated CD4+ T cells for molecular downstream assays
• Fast and efficient depletion of CD4+ T cells from any sample

About Dynabeads® CD4
Dynabeads® CD4 are uniform, superparamagnetic beads (4.5 µm diameter) coated with a primary monoclonal antibody specific for the CD4 membrane antigen mainly expressed on human T helper cells. Because of the bead size, Dynabeads® CD4 can easily and efficiently isolate or deplete cells from viscous samples such as whole blood and bone marrow in about 30 minutes. Dynabeads® CD4 will also afford excellent recovery of high-purity, viable cells for use in downstream molecular studies; for example, those in which cells are to be lysed while still attached to beads, and nucleic acids or proteins further purified. Note that intact cells will not be released from these beads, so if you want to isolate CD4+ cells for cell-based applications, or you need to check your samples with flow cytometry, check out our total human CD4 product offerings.

Magnetic bead–based separation offers easy handling
Dynabeads® CD4 is added to the sample under continuous mixing to optimize the binding of the Dynabeads® to the target cells. By placing the sample on a magnet, it separates the bead-bound target cells from the rest of the sample in just 1–2 minutes. For depletion, remove the supernatant to a new tube for further studies and discard the bead-bound cells. For positive isolation for molecular studies, remove the supernatant and wash the bead-bound cells 2–3 times in buffer to get optimal purity. The cells can be lysed while still attached to the beads and the supernatant transferred to a new tube for downstream molecular analysis. Starting samples can be whole blood, buffy coat, bone marrow, PBMC, or tissue digests.

Learn more about Dynabeads® products
• Find Dynabeads® products for a whole range of applications.
• Find magnets for Dynabeads® separations.

Dynabeads™ CD31 Endothelial Cell Invitrogen™

Isolate endothelial cells directly from tissue digests. Dynabeads® CD31 are ideal for positively isolating endothelial cells for downstream molecular analysis.

• Positively isolate endothelial cells directly from tissue digests for molecular applications
• Establish cultures for endothelial cells
• No columns required

Starting sample:
Tissue digests.

Dynabeads™ CD3 Invitrogen™

Dynabeads® CD3 are superparamagnetic beads coupled with an anti-human CD3 antibody that enable easy isolation or depletion of human CD3+ T cells directly from any sample, including whole blood, bone marrow, buffy coat, mononuclear cells (MNC), and tissue digests. Advantages of Dynabeads® CD3:

• Fast and efficient isolation of CD3+ T cells from any sample—no columns required
• High purity, yield, and viability of positively isolated CD3+ T cells for molecular downstream assays
• Fast and efficient depletion of CD3+ T cells from any sample

About Dynabeads® CD3
Dynabeads® CD3 are uniform, superparamagnetic beads (4.5 µm diameter) coated with a primary monoclonal antibody specific for the CD3 membrane antigen mainly expressed mainly on human mature T cells. Because of the bead size, Dynabeads® CD3 can easily and efficiently isolate or deplete cells from viscous samples such as whole blood and bone marrow in about 30 minutes. Dynabeads® CD3 will also afford excellent recovery of high-purity, viable cells for use in downstream molecular studies; for example, those in which cells are to be lysed while still attached to beads, and nucleic acids or proteins further purified. Note that intact cells will not be released from these beads, so if you want to isolate CD3+ cells for cell-based applications, or you need to check your samples with flow cytometry, check out our total human T cell product offerings.

Magnetic bead–based separation offers easy handling
Dynabeads® CD3 is added to the sample under continuous mixing to optimize the binding of the Dynabeads® to the target cells. By placing the sample on a magnet, it separates the bead-bound target cells from the rest of the sample in just 1–2 minutes. For depletion, remove the supernatant to a new tube for further studies and discard the bead-bound cells. For positive isolation for molecular studies, remove the supernatant and wash the bead-bound cells 2–3 times in buffer to get optimal purity. The cells can be lysed while still attached to the beads and the supernatant transferred to a new tube for downstream molecular analysis. Starting samples can be whole blood, buffy coat, bone marrow, PBMC, or tissue digests.

Learn more about Dynabeads® products
• Find Dynabeads® products for a whole range of applications.
• Find magnets for Dynabeads® separations.

Dynabeads™ CD8 Positive Isolation Kit Invitrogen™

The Dynabeads® CD8 Positive Isolation Kit supplies superparamagnetic beads that enable gentle isolation of pure CD8+ T cells from whole blood, bone marrow, buffy coat, mononuclear cells (MNC), or tissue digests. The cells are released from the beads using the DETACHaBEAD® reagent to obtain bead- and antibody-free cells. Advantages of the Dynabeads® CD8 Positive Isolation Kit:

• Gentle isolation of CD8+ T cells from any sample—no columns required
• Bead- and antibody-free positive isolation of CD8+ cells for any downstream assay

About Dynabeads® CD8 Positive Isolation Kit
The Dynabeads® CD8 Positive Isolation Kit contains uniform, superparamagnetic beads (4.5 µm diameter) covalently coated with a monoclonal antibody specific for the CD8 membrane antigen predominantly expressed on the cytotoxic subset of human T cells. The kit also includes a DETACHaBEAD® reagent that releases the Dynabeads® from the purified CD8+ cells. This gently magnetic isolation process gives highly viable bead- and antibody-free cells to be used in any downstream assay.

Magnetic bead–based separation offers easy handling
Dynabeads® are mixed with the sample in a tube where they bind to the target cells during a short incubation, after which the bead-bound cells are separated from unbound cells using a magnet. The positively isolated cells are then washed, and DETACHaBEAD® reagent is added to gently release the cells from the beads. This magnetic bead-based positive isolation method gives both bead- and antibody-free cells. The isolated CD8+ cells are phenotypically unaltered and suitable for any downstream application. This fast and gentle isolation method does not require the use of columns, and helps ensure high purity and viability of the isolated CD8+ cells. For depletion or isolation of untouched CD8+ cells, select from our range of Human CD8+ T Cell Isolation Products.

Learn more about Dynabeads® products
• Find Dynabeads® products for a whole range of applications.
• Find magnets for Dynabeads® separations.

MagniSort™ Mouse CD335 (NKp46) Positive Selection Kit Invitrogen™

The MagniSort™ Mouse CD335 (NKp46) Positive Selection Kit is designed for the magnetic separation of CD335+ cells by positive selection. It has been optimized for the isolation of CD335+ cells from mouse spleens or lymph nodes utilizing a biotinylated Anti-Mouse CD335+ antibody and streptavidin-coated magnetic beads. CD335+ cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the undesired cells can be separated from CD335+ cells by decanting.

After positive selection, the purity of selected cells can be verified by staining with Anti-Mouse CD49b (Integrin alpha 2), clone DX5, or Anti-Mouse NK1.1, clone PK136, in C57BL, FVB/N and NZB mice.

Reported Application
Magnetic Cell Separation

Dynabeads™ Sheep-Anti Mouse IgG Invitrogen™

Dynabeads® Sheep anti-Mouse IgG binds monoclonal mouse IgG of your choice for specific cell isolation or depletion. All cells from all species except from mouse can be isolated with this product, depending only on the specific antibody of choice.

• Depletion: cells can be depleted from a sample
• Positive isolation: cells can be positively isolated from a sample for molecular applications
• Negative isolation: cells can be negatively isolated from a PBMC sample by adding an antibody cocktail of mouse IgGs towards the unwanted cells. Compatible with flow cytometry.

Note: For a more universal binding of your mouse IgG of any subclass, please use Dynabeads® Pan Mouse Ig (Cat.no. 110-41D or 110-42D).

Starting samples:
Whole blood, PBMC, buffy coat., tissue digests.

MagniSort™ Mouse T cell Enrichment Kit Invitrogen™

The MagniSort™ Mouse T cell Enrichment Kit is designed for the magnetic separation of mouse T cells by negative selection. It has been optimized for the isolation of mouse T cells from spleens or lymph nodes utilizing a biotinylated antibody cocktail and streptavidin-coated magnetic beads. Undesired cells are bound by antibody and then magnetic beads that, when placed in a magnetic field, leave T cells untouched and free in solution.

The MagniSort™ Mouse T cell Enrichment Antibody Cocktail contains the following antibodies:
Anti-Mouse CD11b Biotin
Anti-Mouse CD19 Biotin
Anti-Mouse CD24 Biotin
Anti-Mouse CD45R (B220) Biotin
Anti-Mouse CD49b (Integrin alpha 2) Biotin
Anti-Mouse Ly-6G (Gr-1) Biotin
Anti-Mouse TER-119 Biotin

Reported Application
Magnetic Cell Separation

MagniSort™ Human CD19 Positive Selection Kit Invitrogen™

The MagniSort™ Human CD19 Positive Selection Kit is designed for the magnetic separation of CD19+ cells by positive selection. It has been optimized for the isolation of CD19+ cells from normal human peripheral blood mononuclear cells, utilizing a biotinylated Anti-Human CD19 antibody and streptavidin-coated magnetic beads. CD19+ cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the undesired cells can be separated from CD19+ cells by decanting.

After positive selection, the purity of selected cells can be verified by staining with Anti-Human CD20, clone 2H7.

Reported Application
Magnetic Cell Separation

Dynabeads™ FlowComp™ Human CD8 Kit Invitrogen™

This gentle and easy-to-use kit allows you to isolate flow-compatible human CD8+ T-cells from PBMC or directly from whole blood. Immediately after positive isolation, the beads are released and removed from your cell sample. In this way, you avoid exposing your cells to potentially cytotoxic or immunogenic foreign substances like iron oxides or dextrans, a potential problem when working with biodegradable particles. In addition, your cells will not be exposed to the stress of being passed through a column. You can expect a purity level of >95% human CD8+ cells.

Advantages:

• No risk of your results being affected by the isolation method
• Gentle tube-based method - no columns needed
• High purity and recovery
• Bead-free cells - compatible with flow cytometry analysis
• Compatible with flow cytometry analysis
• Viable, bead-free CD8+ T-cells for direct use in any cell-based assay.

The isolated cells have the presumed profile of memory and naive CD8+ T cells, and show a high proliferation capacity. You will save time and money, and get increased confidence in your results.

Starting sample:
MNC or whole blood.

Capacity:
The kit will process 2 × 109 mononuclear cells (MNC) or 80 ml whole blood

Dynabeads™ Human Treg Expander Gibco™

Dynabeads® Human Treg Expander is intended for activation and expansion of human regulatory T-cells (Treg cells) after isolation with the Dynabeads® Regulatory CD4+CD25+ T Cell Kit (Cat. No. 11363D).

Dynabeads® Human Treg Expander offers a simple method for activation and expansion of Treg cells that does not require antigen presenting cells or antigen. Dynabeads® Human Treg Expander are uniform 4.5 µm, superparamagnetic beads coupled with an optimized mixture of anti-CD3 and anti-CD28 monoclonal antibodies. By combining anti-CD3 and anti-CD28 antibodies on Dynabeads®, the beads will provide both the primary and co-stimulatory signals that are required for activation and expansion of the Treg cells.

Starting Sample
Human regulatory T-cells isolated with the Dynabeads® Regulatory CD4+CD25+ T Cell Kit (Cat. No. 11363D).

Dynabeads™ Human T-Expander CD3/CD28 Gibco™

Dynabeads® Human T-Expander CD3/CD28 is intended for separation, activation and expansion of human T cells. Dynabeads® Human T-Expander CD3/CD28 is the research grade version of Dynabeads® CD3/CD28 CTS™. Containing the same amount and ratio of antibodies from the same clones as Dynabeads® CD3/CD28 CTS™, Dynabeads® Human T-Expander CD3/CD28 are intended for small scale pre-clinical research. Dynabeads® Human T-Expander CD3/CD28 offers a simple method for activation and expansion of T-cells that does not require antigen presenting cells or antigen. By combining anti-CD3 and anti-CD28 antibodies on Dynabeads®, the beads provide both the primary and co-stimulatory signals that are required for activation and expansion of T cells.

Applications:
Dynabeads® Human T-Expander CD3/CD28 is the research grade version of Dynabeads® CD3/CD28 CTS™. The Dynabeads® CD3/CD28 CTS™ technology has been successfully used in ex vivo separation, activation and expansion of T-cells in a number of studies, including studies on HIV infection and cancer. Other potential applications for this novel technology are treatment of autoimmune and infectious diseases and prevention of complications associated with chemotherapy and allogeneic transplantation.

Dynabeads™ CD45 Invitrogen™

Dynabeads® CD45 are superparamagnetic beads covalently coupled to anti-human CD45 antibody that enable isolation or depletion of CD45+ leucocytes directly from whole blood, buffy coat, or MNC suspensions. Dynabeads® CD45 can also be used to enrich epithelial tumor cells. The CD45 antigen is weakly expressed on myeloid cells. To efficiently deplete or isolate all leucocytes including myeloid cells, use Dynabeads® CD15 (Cat. No. 11137D) in combination with Dynabeads® CD45. Advantages of Dynabeads® CD45:

• Efficient depletion of human leucocytes
• Isolation of leucocytes directly from whole blood for molecular applications
• Enrichment of circulating epithelial tumor cells

About Dynabeads® CD45
Dynabeads® CD45 are uniform, superparamagnetic beads (4.5 µm diameter) coated with a primary monoclonal mouse IgG2a antibody specific for a CD45 membrane antigen common to all known isoforms of CD45. CD45 is expressed on all human leucocytes. Because of the bead size, Dynabeads® CD45 can easily and efficiently isolate or deplete cells from viscous samples such as whole blood and bone marrow in about 30 minutes. Dynabeads® CD45 is often used for enrichment of circulating non-hematopoietic tumor cells, by depletion of all CD45+ leucecytes from a MNC sample. Positively isolated cells can be used for downstream molecular studies; for example, those in which cells are to be lysed while still attached to beads, and nucleic acids or proteins further purified. Note that intact cells will not be released from these beads, and we do not recommend to analyze the bead-bound cells with a flow cytometer.

Magnetic bead–based separation offers easy handling
Dynabeads® CD45 is added to the sample under continuous mixing to optimize the binding of the Dynabeads® to the target cells. By placing the sample on a magnet it separates the bead-bound target cells from the rest of the sample in just 1–2 minutes. For depletion, remove the supernatant to a new tube for further studies and discard the bead-bound cells. For positive isolation for molecular studies, remove the supernatant and wash the bead-bound cells 2–3 times in buffer to get optimal purity. The cells can be lysed while still attached to the beads and the supernatant transferred to a new tube for downstream molecular analysis. Starting samples can be whole blood, buffy coat, bone marrow, PBMC, or tissue digests.

Learn more about Dynabeads® products
• Find Dynabeads® products for a whole range of applications.
• Find magnets for Dynabeads® separations.

MagniSort™ Human CD3 Positive Selection Kit Invitrogen™

The MagniSort™ Human CD3 Positive Selection Kit is designed for the magnetic separation of CD3+ cells by positive selection. It has been optimized for the isolation of CD3+ cells from human peripheral blood monocuclear cells, utilizing a biotinylated Anti-Human CD3 antibody and streptavidin-coated magnetic beads. CD3+ cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the undesired cells can be separated from CD3+ cells by decanting.

After positive selection, the purity of selected cells can be verified by staining with Anti-Human CD3, clone UCHT1 or SK7.

Reported Application
Magnetic Cell Separation

MagniSort™ Mouse CD19 Positive Selection Kit Invitrogen™

The MagniSort™ Mouse CD19 Positive Selection Kit is designed for the magnetic separation of CD19+ cells by positive selection. It has been optimized for the isolation of CD19+ cells from mouse spleens, bone marrow, or lymph nodes utilizing a biotinylated Anti-Mouse CD19 antibody and streptavidin-coated magnetic beads. CD19+ cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the undesired cells can be separated from CD19+ cells by decanting.

After positive selection, the purity of selected cells can be verified by staining with Anti-Human/Mouse CD45R (B220), clone RA3-6B2.

Reported Application
Magnetic Cell Separation

Results per page
    spinner