Shop All Magnetic Beads for Cell Isolation & Expansion

MagniSort™ Mouse Hematopoietic Lineage Depletion Kit (Invitrogen™)

The MagniSort™ Mouse Hematopoietic Lineage Depletion Kit is designed for the magnetic depletion of mouse hematopoietic cells with committed lineages from mouse bone marrow. It utilizes a cocktail of antibody specific for hematopoietic lineages and streptavidin-coated magnetic beads. Lineage committed cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the lineage negative cells can be separated from lineage committed cells by decanting.

The MagniSort™ Mouse Hematopoietic Lineage Depletion Antibody Cocktail contains the following antibodies:
Anti-Mouse CD2 Biotin
Anti-Mouse CD3 Biotin
Anti-Mouse CD5 Biotin
Anti-Mouse CD11b Biotin
Anti-Mouse CD19 Biotin
Anti-Mouse CD45R Biotin
Anti-Mouse Ly-6G (Gr-1) Biotin
Anti-Mouse TER-119 Biotin

After depletion, the enrichment of multi-potent progenitor cells can be verified by staining with Anti-Mouse CD34 (clone RAM34), and the enrichment of CD34- hematopoietic stem cells can be verified by additional staining with Anti-Mouse CD150 (clone mShad150) and Anti-Mouse CD48 (clone HM48-1).

Reported Application
Magnetic Cell Separation

Dynabeads™ Untouched™ Human Monocytes Kit (Invitrogen™)

The Dynabeads® Untouched™ Human Monocytes Kit is used to isolate pure and viable monocytes from PBMC by negative isolation. The kit depletes T cells, B cells, NK cells, dendritic cells, granulocytes, and erythrocytes, while the negatively isolated human monocytes are left in the sample. The isolated monocytes cells are in perfect shape for any functional assay and application. Advantages of the Dynabeads® Untouched™ Human Monocytes Kit:

• High purity, recovery, and viability of human monocytes
• Easy to use and to scale up
• No columns required
Magnetic bead-based separation offers easy handling
An antibody mix towards the non-monocytes is added to the sample and allowed to bind to the cells. After a quick wash, the Dynabeads® are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated human monocytes can be directly analyzed in a flow cytometer and used in any application.

Learn more about Dynabeads® products
• Find Dynabeads® products for a whole range of applications.
• Find magnets for Dynabeads® separations.

MagniSort™ Human Hematopoietic Lineage Depletion Kit (Invitrogen™)

The MagniSort™ Human Hematopoietic Lineage Depletion Kit is designed for the magnetic depletion of lineage positive cells from normal human peripheral blood mononuclear cells. The kit utilizes a biotinylated antibody cocktail and streptavidin-coated magnetic beads. Undesired cells, including T cells, B cells, NK cells, myeloid cells, granulocytes, and red blood cells, are bound by antibody and then magnetic beads that, when placed in a magnetic field, leave lineage negative cells untouched and free in solution.

The MagniSort™ Human Hematopoietic Lineage Antibody Cocktail contains the following antibodies:
Anti-Human CD2 Biotin
Anti-Human CD3 Biotin
Anti-Human CD10 Biotin
Anti-Human CD11b Biotin
Anti-Human CD14 Biotin
Anti-Human CD16 Biotin
Anti-Human CD19 Biotin
Anti-Human CD56 Biotin
Anti-Human CD123 Biotin
Anti-Human CD235a Biotin

Reported Application
Magnetic Cell Separation

MagniSort™ Mouse CD4 T cell Enrichment Kit (Invitrogen™)

The MagniSort™ Mouse CD4 T cell Enrichment Kit is designed for the magnetic separation of mouse CD4 T cells by negative selection. It has been optimized for the isolation of mouse CD4 T cells from spleens or lymph nodes utilizing a biotinylated antibody cocktail and streptavidin-coated magnetic beads. Undesired cells are bound by antibody and then magnetic beads that, when placed in a magnetic field, leave CD4 T cells untouched and free in solution.

The MagniSort™ Mouse CD4 T cell Enrichment Antibody Cocktail contains the following antibodies:
Anti-Mouse CD8 Biotin
Anti-Mouse CD11b Biotin
Anti-Mouse CD19 Biotin
Anti-Mouse CD24 Biotin
Anti-Mouse CD45R (B220) Biotin
Anti-Mouse CD49b (Integrin alpha 2) Biotin
Anti-Mouse Ly-6G (Gr-1) Biotin
Anti-Mouse γδ TCR Biotin
Anti-Mouse TER-119 Biotin

Reported Application
Magnetic Cell Separation

MagniSort™ Human pan-Monocyte Enrichment Kit (Invitrogen™)

The MagniSort™ Human Pan-Monocyte Enrichment Kit is designed for the magnetic separation of conventional CD14+ and unconventional CD16+CD14- monocytes by negative selection. It has been optimized for the isolation of human pan-monocytes from normal human peripheral blood mononuclear cells utilizing a biotinylated antibody cocktail and streptavidin-coated magnetic beads. Undesired cells, including CD16+ NK cells, are bound by antibody and then magnetic beads that, when placed in a magnetic field, leaving monocytes untouched and free in solution.

The MagniSort™ Human Pan-Monocyte Enrichment Antibody Cocktail contains the following antibodies:
Anti-Human CD2 Biotin
Anti-Human CD3 Biotin
Anti-Human CD7 Biotin
Anti-Human CD19 Biotin
Anti-Human CD20 Biotin
Anti-Human CD56 Biotin
Anti-Human CD57 Biotin
Anti-Human CD123 Biotin
Anti-Human CD235a Biotin
Human Fc Receptor Binding Inhibitor Purified


Reported Application
Magnetic Cell Separation

Dynabeads™ FlowComp™ Human CD8 Kit (Invitrogen™)

This gentle and easy-to-use kit allows you to isolate flow-compatible human CD8+ T-cells from PBMC or directly from whole blood. Immediately after positive isolation, the beads are released and removed from your cell sample. In this way, you avoid exposing your cells to potentially cytotoxic or immunogenic foreign substances like iron oxides or dextrans, a potential problem when working with biodegradable particles. In addition, your cells will not be exposed to the stress of being passed through a column. You can expect a purity level of >95% human CD8+ cells.

Advantages:

• No risk of your results being affected by the isolation method
• Gentle tube-based method - no columns needed
• High purity and recovery
• Bead-free cells - compatible with flow cytometry analysis
• Compatible with flow cytometry analysis
• Viable, bead-free CD8+ T-cells for direct use in any cell-based assay.

The isolated cells have the presumed profile of memory and naive CD8+ T cells, and show a high proliferation capacity. You will save time and money, and get increased confidence in your results.

Starting sample:
MNC or whole blood.

Capacity:
The kit will process 2 × 109 mononuclear cells (MNC) or 80 ml whole blood

Dynabeads™ Untouched™ Human B Cells Kit (Invitrogen™)

Use this kit to isolate pure and viable untouched B cells from PBMC by negative isolation. The kit depletes T cells, NK cells, monocytes, platelets, dendritic cells, granulocytes and erythrocytes. The negatively isolated human B cells are left in the sample and have not been in contact with the Dynabeads. The bead and antibody-free B cells are truly untouched, and in perfect shape for any functional assay and application.

• High purity, recovery and viability of human untouched B cells
• Easy to use and to scale up
• No columns required

An antibody mix towards the non-B cells is added to the sample and allowed to bind to the cells. Dynabeads® are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched human B cells can be directly analyzed in a flow cytometer and used in any application.

Starting Sample:
PBMC

MagniSort™ Mouse CD8 T cell Enrichment Kit (Invitrogen™)

The MagniSort™ Mouse CD8 T cell Enrichment Kit is designed for the magnetic separation of mouse CD8 T cells by negative selection. It has been optimized for the isolation of mouse CD8 T cells from spleens or lymph nodes utilizing a biotinylated antibody cocktail and streptavidin-coated magnetic beads. Undesired cells are bound by antibody and then magnetic beads that, when placed in a magnetic field, leave CD8 T cells untouched and free in solution.

The MagniSort™ Mouse CD8 T cell Enrichment Antibody Cocktail contains the following antibodies:
Anti-Mouse CD4 Biotin
Anti-Mouse CD11b Biotin
Anti-Mouse CD19 Biotin
Anti-Mouse CD24 Biotin
Anti-Mouse CD45R (B220) Biotin
Anti-Mouse CD49b (Integrin alpha 2) Biotin
Anti-Mouse Ly-6G (Gr-1) Biotin
Anti-Mouse γδ TCR Biotin
Anti-Mouse TER-119 Biotin

Reported Application
Magnetic Cell Separation

Dynabeads™ FlowComp™ Mouse CD4 Kit (Invitrogen™)

This gentle and easy-to-use kit allows you to isolate flow-compatible mouse CD4+ T cells. Immediately after positive isolation, the beads are released and removed from your cell sample. In this way, you avoid exposing your cells to potentially cytotoxic or immunogenic foreign substances like iron oxides or dextrans, a potential problem when working with biodegradable particles. In addition, your cells will not be exposed to the stress of being passed through a column.

You can expect a purity level of >85% and viability of >85% of mouse CD4+ cells.

Advantages:

• No risk of your results being affected by the isolation method
• Gentle tube-based method - no columns needed
• High purity, recovery and viability
• Bead-free cells - compatible with flow cytometry analysis
• Viable, bead-free CD4+ T-cells for direct use in any cell-based assay.

The isolated cells have the presumed profile of memory and naive CD4+ T cells, and show a high proliferation capacity. You will save time and money, and get increased confidence in your results.

Starting sample: Spleen or lymph node cells

Dynabeads™ CD8 (Invitrogen™)

Dynabeads® CD8 are superparamagnetic beads coupled with an anti-human CD8 antibody that enable easy isolation or depletion of human CD8+ T cells directly from any sample, including whole blood, bone marrow, buffy coat, mononuclear cells (MNC), and tissue digests. Advantages of Dynabeads® CD8:

• Fast and efficient isolation of CD8+ T cells from any sample—no columns required
• High purity, yield, and viability of positively isolated CD8+ T cells for molecular downstream assays
• Fast and efficient depletion of CD8+ T cells from any sample

About Dynabeads® CD8
Dynabeads® CD8 are uniform, superparamagnetic beads (4.5 µm diameter) coated with a primary monoclonal antibody specific for the CD8 membrane antigen mainly expressed on human cytotoxic T cells. Because of the bead size, Dynabeads® CD8 can easily and efficiently isolate or deplete cells from viscous samples such as whole blood and bone marrow in about 30 minutes. Dynabeads® CD8 will also afford excellent recovery of high-purity, viable cells for use in downstream molecular studies; for example, those in which cells are to be lysed while still attached to beads, and nucleic acids or proteins further purified. Note that intact cells will not be released from these beads, so if you want to isolate CD8+ cells for cell-based applications, or you need to check your samples with flow cytometry, check out our total human CD8 product offerings.

Magnetic bead–based separation offers easy handling
Dynabeads® CD8 is added to the sample under continuous mixing to optimize the binding of the Dynabeads® to the target cells. By placing the sample on a magnet, it separates the bead-bound target cells from the rest of the sample in just 1–2 minutes. For depletion, remove the supernatant to a new tube for further studies and discard the bead-bound cells. For positive isolation for molecular studies, remove the supernatant and wash the bead-bound cells 2–3 times in buffer to get optimal purity. The cells can be lysed while still attached to the beads and the supernatant transferred to a new tube for downstream molecular analysis. Starting samples can be whole blood, buffy coat, bone marrow, PBMC, or tissue digests.

Learn more about Dynabeads® products
• Find Dynabeads® products for a whole range of applicationsa
• Find magnets for Dynabeads® separations.

MagniSort™ Mouse CD45 Depletion Kit (Invitrogen™)

The MagniSort™ Mouse CD45 Depletion Kit is designed for the magnetic depletion of mouse CD45+ cells from mouse spleens, bone marrow or digested lung tissue. It utilizes a biotinylated Anti-Mouse CD45 antibody and streptavidin-coated magnetic beads. CD45+ cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the CD45- cells can be separated from CD45+ cells by decanting.

After depletion, the purity of enriched CD45- cells can be verified by staining with Anti-Mouse CD45, clone 30-F11.

Reported Application
Magnetic Cell Separation

Dynabeads™ Untouched™ Human CD8 T Cells Kit (Invitrogen™)

The Dynabeads® Untouched™ Human CD8 T Cells Kit is used to isolate pure and viable untouched CD8+ T cells from PBMC by negative isolation. The kit depletes CD4+ T cells, B cells, NK cells, monocytes, platelets, dendritic cells, granulocytes, and erythrocytes. The negatively isolated human CD8+ T-cells are left in the sample and have not been in contact with the Dynabeads®. The bead- and antibody-free CD8+ T cells are truly untouched, and in perfect shape for any functional assay or application.

Advantages of the Dynabeads® Untouched™ Human CD8 T Cells Kit:

• High purity, recovery, and viability of human untouched CD8+ T cells
• Easy to use and to scale up
• No columns required

Magnetic bead-based Separation offers easy handling
An antibody mix towards the non-CD8 cells is added to the sample and allowed to bind to the cells. Dynabeads® are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched human CD8+ T cells can be directly analyzed in a flow cytometer and used in any application. For depletion or positive isolation of CD8+ cells, select from our range of Human CD8+ T Cell Isolation Products.

Learn more about Dynabeads® products
• Find Dynabeads® products for a whole range of applications.
• Find magnets for Dynabeads® separations.

MagniSort™ Mouse CD4 Naïve T cell Enrichment Kit (Invitrogen™)

The MagniSort™ Mouse CD4 Naïve T cell Enrichment Kit is designed for the magnetic separation of CD4 naïve T cells by negative selection. It has been optimized for the isolation of mouse CD4 naïve T cells from spleens or lymph nodes utilizing a biotinylated antibody cocktail and streptavidin-coated magnetic beads. Undesired cells are bound by antibody and then magnetic beads that, when placed in a magnetic field, leave CD4 naïve T cells untouched and free in solution.

The MagniSort™ Mouse CD4 Naïve T cell Enrichment Antibody Cocktail contains the following antibodies:
Anti-Mouse CD8 Biotin
Anti-Mouse CD11b Biotin
Anti-Mouse CD19 Biotin
Anti-Mouse CD24 Biotin
Anti-Mouse CD44 Biotin
Anti-Mouse CD45R (B220) Biotin
Anti-Mouse CD49b (Integrin alpha 2) Biotin
Anti-Mouse Ly-6G (Gr-1) Biotin
Anti-Mouse γδ TCR Biotin
Anti-Mouse TER-119 Biotin

Reported Application
Magnetic Cell Separation

Dynabeads™ CD4 (Invitrogen™)

Dynabeads® CD4 are superparamagnetic beads coupled with an anti-human CD4 antibody that enable easy isolation or deplettion of human CD4+ T cells directly from any sample, including whole blood, bone marrow, buffy coat, mononuclear cells (MNC), and tissue digests. Advantages of Dynabeads® CD4:

• Fast and efficient isolation of CD4+ T cells from any sample—no columns required
• High purity, yield, and viability of positively isolated CD4+ T cells for molecular downstream assays
• Fast and efficient depletion of CD4+ T cells from any sample

About Dynabeads® CD4
Dynabeads® CD4 are uniform, superparamagnetic beads (4.5 µm diameter) coated with a primary monoclonal antibody specific for the CD4 membrane antigen mainly expressed on human T helper cells. Because of the bead size, Dynabeads® CD4 can easily and efficiently isolate or deplete cells from viscous samples such as whole blood and bone marrow in about 30 minutes. Dynabeads® CD4 will also afford excellent recovery of high-purity, viable cells for use in downstream molecular studies; for example, those in which cells are to be lysed while still attached to beads, and nucleic acids or proteins further purified. Note that intact cells will not be released from these beads, so if you want to isolate CD4+ cells for cell-based applications, or you need to check your samples with flow cytometry, check out our total human CD4 product offerings.

Magnetic bead–based separation offers easy handling
Dynabeads® CD4 is added to the sample under continuous mixing to optimize the binding of the Dynabeads® to the target cells. By placing the sample on a magnet, it separates the bead-bound target cells from the rest of the sample in just 1–2 minutes. For depletion, remove the supernatant to a new tube for further studies and discard the bead-bound cells. For positive isolation for molecular studies, remove the supernatant and wash the bead-bound cells 2–3 times in buffer to get optimal purity. The cells can be lysed while still attached to the beads and the supernatant transferred to a new tube for downstream molecular analysis. Starting samples can be whole blood, buffy coat, bone marrow, PBMC, or tissue digests.

Learn more about Dynabeads® products
• Find Dynabeads® products for a whole range of applications.
• Find magnets for Dynabeads® separations.

Dynabeads™ CD31 Endothelial Cell (Invitrogen™)

Isolate endothelial cells directly from tissue digests. Dynabeads® CD31 are ideal for positively isolating endothelial cells for downstream molecular analysis.

• Positively isolate endothelial cells directly from tissue digests for molecular applications
• Establish cultures for endothelial cells
• No columns required

Starting sample:
Tissue digests.