Shop All Magnetic Beads for Cell Isolation & Expansion

Dynabeads™ Untouched™ Human CD8 T Cells Kit (Invitrogen™)

The Dynabeads® Untouched™ Human CD8 T Cells Kit is used to isolate pure and viable untouched CD8+ T cells from PBMC by negative isolation. The kit depletes CD4+ T cells, B cells, NK cells, monocytes, platelets, dendritic cells, granulocytes, and erythrocytes. The negatively isolated human CD8+ T-cells are left in the sample and have not been in contact with the Dynabeads®. The bead- and antibody-free CD8+ T cells are truly untouched, and in perfect shape for any functional assay or application.

Advantages of the Dynabeads® Untouched™ Human CD8 T Cells Kit:

• High purity, recovery, and viability of human untouched CD8+ T cells
• Easy to use and to scale up
• No columns required

Magnetic bead-based Separation offers easy handling
An antibody mix towards the non-CD8 cells is added to the sample and allowed to bind to the cells. Dynabeads® are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched human CD8+ T cells can be directly analyzed in a flow cytometer and used in any application. For depletion or positive isolation of CD8+ cells, select from our range of Human CD8+ T Cell Isolation Products.

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MagniSort™ Human CD4 Memory T cell Enrichment Kit (Invitrogen™)

The MagniSort™ Human CD4 Memory T cell Enrichment Kit is designed for the magnetic separation of CD4 memory T cells by negative selection. It has been optimized for the isolation of human CD4 memory T cells from normal human peripheral blood mononuclear cells utilizing a biotinylated antibody cocktail and streptavidin-coated magnetic beads. Undesired cells are bound by antibody and then magnetic beads that, when placed in a magnetic field, leave CD4 memory T cells untouched and free in solution.

The MagniSort™ Human CD4 Memory T cell Enrichment Antibody Cocktail contains the following antibodies:
Anti-Human CD8 Biotin
Anti-Human CD11b Biotin
Anti-Human CD14 Biotin
Anti-Human CD16 Biotin
Anti-Human CD19 Biotin
Anti-Human CD20 Biotin
Anti-Human CD36 Biotin
Anti-Human CD45RA Biotin
Anti-Human CD56 Biotin
Anti-Human CD123 Biotin
Anti-Human CD235a Biotin
Anti-Human γδ TCR Biotin

Reported Application
Magnetic Cell Separation

Dynabeads™ FlowComp™ Flexi Kit (Invitrogen™)

This gentle, easy-to-use and highly flexible kit allows you to positively isolate bead-free and flow-compatible cells in combination with your own antibody of choice. Now you can isolate practically any cell type from any species or starting sample using your own antibody. Immediately after positive isolation, the beads are released and removed from your cell sample. In this way, you avoid exposing your cells to potentially cytotoxic or immunogenic foreign substances like iron oxides or dextrans, a potential problem when working with biodegradable particles. In addition, your cells will not be exposed to the stress of being passed through a column.

The kit supplies you with everything you need for DSB-X™ biotinylation of your antibodies and subsequent isolation of your cell type of interest. This technology has been successfully used to isolate a number of cell subsets including human and mouse T cells, B cells, NK cells, monocytes, and endothelial cells.

Advantages:

• Combine with your antibody of choice to isolate any cell type
• No risk of your results being affected by the isolation method
• Gentle tube-based method - no columns needed
• High purity, recovery and viability
• Bead-free cells - compatible with flow cytometry analysis or for direct use in any cell-based assay

Starting sample:
Whole blood, PBMC/MNC, single cell suspensions from lymph nodes, spleen or other tissues.

Capacity:
The kit will process 2 x 109 mononuclear cells (MNC). Please note that the choice of antibody clone is of key importance for successful isolation (see package insert for technical recommendations).

Concentration:
Supplied at 15 mg beads per ml in phosphate buffered saline (PBS), pH 7.4, containing 0.1% bovine serum albumin (BSA) and 0.02% sodium azide.

MagniSort™ Mouse CD335 (NKp46) Positive Selection Kit (Invitrogen™)

The MagniSort™ Mouse CD335 (NKp46) Positive Selection Kit is designed for the magnetic separation of CD335+ cells by positive selection. It has been optimized for the isolation of CD335+ cells from mouse spleens or lymph nodes utilizing a biotinylated Anti-Mouse CD335+ antibody and streptavidin-coated magnetic beads. CD335+ cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the undesired cells can be separated from CD335+ cells by decanting.

After positive selection, the purity of selected cells can be verified by staining with Anti-Mouse CD49b (Integrin alpha 2), clone DX5, or Anti-Mouse NK1.1, clone PK136, in C57BL, FVB/N and NZB mice.

Reported Application
Magnetic Cell Separation

CTS™ (Cell Therapy Systems) Dynabeads™ CD3/CD28 (Gibco™)

CTS™ Dynabeads™ CD3/CD28 are intended for ex vivo isolation, activation, and expansion of human T-cells in translational research. The technology—previously known as Xcyte™ Dynabeads™ or Dynabeads™ ClinExVivo™ CD3/CD28—has been used in a number of clinical studies. By combining anti-CD3 and anti-CD28 antibodies on Dynabeads, the beads provide both the primary and co-stimulatory signals that are required for activation and expansion of T cells. CTS Dynabeads CD3/CD28 are manufactured and controlled according to ISO 9001:2000 and ISO 13485:2012 requirements. A Device Master File is held with the FDA for cross-referencing in IND applications, and a CoA is available on request. In the US, CTS Dynabeads CD3/CD28 are available for clinical use only under an approved Investigational New Drug (IND) application. Dynabeads™ Human T-Expander CD3/CD28 (Cat. No. 11141D) is the research grade version of CTS Dynabeads CD3/CD28, containing the same amount and ratio of antibodies from the same clones as CTS Dynabeads CD3/CD28, and intended for small scale pre-clinical research.

Applications:
The CTS Dynabeads CD3/CD28 technology has been successfully used in ex vivo isolation, activation, and expansion of T-cells in a number of studies, including studies on HIV infection and cancer. Other potential applications for this novel technology include research into treatment of autoimmune and infectious diseases and prevention of complications associated with chemotherapy and allogeneic stem cell transplantation.

MagniSort™ Human CD4 Naïve T cell Enrichment Kit (Invitrogen™)

The MagniSort™ Human CD4 Naïve T cell Enrichment Kit is designed for the magnetic separation of CD4 naïve T cells by negative selection. It has been optimized for the isolation of human CD4 naïve T cells from normal human peripheral blood mononuclear cells utilizing a biotinylated antibody cocktail and streptavidin-coated magnetic beads. Undesired cells are bound by antibody and then magnetic beads that, when placed in a magnetic field, leave CD4 naïve T cells untouched and free in solution.

The MagniSort™ Human CD4 Naïve T cell Enrichment Antibody Cocktail contains the following antibodies:
Anti-Human CD8 Biotin
Anti-Human CD11b Biotin
Anti-Human CD14 Biotin
Anti-Human CD16 Biotin
Anti-Human CD19 Biotin
Anti-Human CD20 Biotin
Anti-Human CD36 Biotin
Anti-Human CD45RO Biotin
Anti-Human CD56 Biotin
Anti-Human CD123 Biotin
Anti-Human CD235a Biotin
Anti-Human γδ TCR Biotin

Reported Application
Magnetic Cell Separation

MagniSort™ Mouse CD4 T cell Enrichment Kit (Invitrogen™)

The MagniSort™ Mouse CD4 T cell Enrichment Kit is designed for the magnetic separation of mouse CD4 T cells by negative selection. It has been optimized for the isolation of mouse CD4 T cells from spleens or lymph nodes utilizing a biotinylated antibody cocktail and streptavidin-coated magnetic beads. Undesired cells are bound by antibody and then magnetic beads that, when placed in a magnetic field, leave CD4 T cells untouched and free in solution.

The MagniSort™ Mouse CD4 T cell Enrichment Antibody Cocktail contains the following antibodies:
Anti-Mouse CD8 Biotin
Anti-Mouse CD11b Biotin
Anti-Mouse CD19 Biotin
Anti-Mouse CD24 Biotin
Anti-Mouse CD45R (B220) Biotin
Anti-Mouse CD49b (Integrin alpha 2) Biotin
Anti-Mouse Ly-6G (Gr-1) Biotin
Anti-Mouse γδ TCR Biotin
Anti-Mouse TER-119 Biotin

Reported Application
Magnetic Cell Separation

Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation (Gibco™)

Dynabeads® Human T-Activator CD3/CD28 are for the activation and expansion of human T cells. Advantages of Dynabeads® Human T-Activator CD3/CD28:

• Activation of T cells without the need for feeder cells
• Activated cells that retain in vivo-like function
• Maximum reproducibility without contamination by soluble antibodies or mitogens
• From 100- to 1,000-fold T cell expansion

About Dynabeads® Human T-Activator CD3/CD28
Dynabeads® Human T-Activator CD3/CD28 offer a simple method for activation and expansion of T cells that does not require feeder cells (antigen-presenting cells) or antigen. The uniform 4.5 µm diameter, inert, superparamagnetic beads are similar in size to antigen-presenting cells, and are covalently coupled to anti-CD3 and anti-CD28 antibodies. These two antibodies provide primary and co-stimulatory signals, optimized for efficient T cell activation and expansion. Expansion of the T cell population can be stimulated using recombinant IL-2, and after activation or expansion, the magnetic beads can be easily removed using a DynaMag™ magnet. For expansion of antigen-specific T cells from T cell clones or T cell lines, we recommend using Dynabeads® Human T-Activator CD3/CD28/CD137.

Starting samples
Start with mononuclear cells (MNCs)/peripheral blood mononuclear cells (PBMCs) from either whole blood or buffy coat, or T cell subsets including CD3+ T cells, CD4+ or CD8+ T cells, or regulatory T cells.

Downstream applications
The activated T cells can be analyzed shortly after activation (for transfection/transduction or to study TCR signaling, proteomics, gene expression, etc.). T cells can be left in culture to differentiate into T helper cell subsets or expansion of polyclonal/antigen-specific T cells.

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MagniSort™ Human CD3 Positive Selection Kit (Invitrogen™)

The MagniSort™ Human CD3 Positive Selection Kit is designed for the magnetic separation of CD3+ cells by positive selection. It has been optimized for the isolation of CD3+ cells from human peripheral blood monocuclear cells, utilizing a biotinylated Anti-Human CD3 antibody and streptavidin-coated magnetic beads. CD3+ cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the undesired cells can be separated from CD3+ cells by decanting.

After positive selection, the purity of selected cells can be verified by staining with Anti-Human CD3, clone UCHT1 or SK7.

Reported Application
Magnetic Cell Separation

Dynabeads™ Untouched™ Human NK Cells Kit (Invitrogen™)

Use this kit to isolate pure and viable untouched NK cells from PBMC by negative isolation. The kit depletes T cells, B cells, monocytes, platelets, dendritic cells, granulocytes and erythrocytes. The negatively isolated human NK cells are left in the sample and have not been in contact with the Dynabeads. The bead and antibody-free NK cells are truly untouched, and in perfect shape for any functional assay and application.

• High purity, recovery and viability of human untouched NK cells
• Easy to use and to scale up
• No columns required

Methodology:
An antibody mix towards the non-NK cells is added to the sample and allowed to bind to the cells. Dynabeads® are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched human NK cells can be directly analyzed in a flow cytometer and used in any application.

Starting Sample:
PBMC

Dynabeads™ CD8 Positive Isolation Kit (Invitrogen™)

The Dynabeads® CD8 Positive Isolation Kit supplies superparamagnetic beads that enable gentle isolation of pure CD8+ T cells from whole blood, bone marrow, buffy coat, mononuclear cells (MNC), or tissue digests. The cells are released from the beads using the DETACHaBEAD® reagent to obtain bead- and antibody-free cells. Advantages of the Dynabeads® CD8 Positive Isolation Kit:

• Gentle isolation of CD8+ T cells from any sample—no columns required
• Bead- and antibody-free positive isolation of CD8+ cells for any downstream assay

About Dynabeads® CD8 Positive Isolation Kit
The Dynabeads® CD8 Positive Isolation Kit contains uniform, superparamagnetic beads (4.5 µm diameter) covalently coated with a monoclonal antibody specific for the CD8 membrane antigen predominantly expressed on the cytotoxic subset of human T cells. The kit also includes a DETACHaBEAD® reagent that releases the Dynabeads® from the purified CD8+ cells. This gently magnetic isolation process gives highly viable bead- and antibody-free cells to be used in any downstream assay.

Magnetic bead–based separation offers easy handling
Dynabeads® are mixed with the sample in a tube where they bind to the target cells during a short incubation, after which the bead-bound cells are separated from unbound cells using a magnet. The positively isolated cells are then washed, and DETACHaBEAD® reagent is added to gently release the cells from the beads. This magnetic bead-based positive isolation method gives both bead- and antibody-free cells. The isolated CD8+ cells are phenotypically unaltered and suitable for any downstream application. This fast and gentle isolation method does not require the use of columns, and helps ensure high purity and viability of the isolated CD8+ cells. For depletion or isolation of untouched CD8+ cells, select from our range of Human CD8+ T Cell Isolation Products.

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MagniSort™ Human CD8 Memory T cell Enrichment Kit (Invitrogen™)

The MagniSort™ Human CD8 Memory T cell Enrichment Kit is designed for the magnetic separation of CD8 memory T cells by negative selection. It has been optimized for the isolation of human CD8 memory T cells from normal human peripheral blood mononuclear cells utilizing a biotinylated antibody cocktail and streptavidin-coated magnetic beads. Undesired cells are bound by antibody and then magnetic beads that, when placed in a magnetic field, leave CD8 memory T cells untouched and free in solution.

The MagniSort™ Human CD8 Memory T cell Enrichment Antibody Cocktail contains the following antibodies:
Anti-Human CD4 Biotin
Anti-Human CD11b Biotin
Anti-Human CD14 Biotin
Anti-Human CD16 Biotin
Anti-Human CD19 Biotin
Anti-Human CD20 Biotin
Anti-Human CD36 Biotin
Anti-Human CD45RA Biotin
Anti-Human CD56 Biotin
Anti-Human CD123 Biotin
Anti-Human CD235a Biotin
Anti-Human γδ TCR Biotin

Reported Application
Magnetic Cell Separation

Dynabeads™ Untouched™ Mouse CD8 Cells Kit (Invitrogen™)

Dynabeads® Untouched™ Mouse CD8 Cells supplies both 4.5 µm superparamagnetic Dynabeads® (with a polyclonal sheep anti-rat IgG antibody covalently bound to the bead surface) and a monoclonal Antibody Mix, together designed for the depletion of CD8-negative leucocytes from mouse spleen or lymph node single-cell suspensions. Following the depletion procedure, the negatively isolated, untouched target mouse CD8+ cells are pure, viable, and ready to be used in any downstream application.

• Rapid isolation of untouched mouse CD8+ T cells—no columns required
• Pure and viable mouse CD8+ T cells are ready for any downstream application

Excellent yield, purity, and viability of recovered cells
The Antibody Mix contains a cocktail of rat IgGs that binds mouse CD4+ T cells, B cells, NK cells, monocytes/macrophages, dendritic cells, erythrocytes, and granulocytes. The Antibody Mix is first added to the sample in a tube to bind the unwanted CD8-negative cells. The cells are then washed to remove excess antibodies. Mouse Depletion Dynabeads® (coated with a rat IgG antibody) are added to bind the antibody-labeled, CD8-negative cells. After a short incubation, the bead-bound cells are then separated from unbound cells in 1–2 minutes using a magnet. The purified target mouse CD8+ cells in the supernatant can be transferred to a new tube for use in any downstream application.

This fast and gentle isolation method does not require the use of columns, and helps ensure high purity, recovery and viability of the untouched CD8+ cells. The protocol can be scaled up according to your sample size.

Learn more about Dynabeads® products
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• Find magnets for Dynabeads® separations.

MagniSort™ Mouse CD8 T cell Enrichment Kit (Invitrogen™)

The MagniSort™ Mouse CD8 T cell Enrichment Kit is designed for the magnetic separation of mouse CD8 T cells by negative selection. It has been optimized for the isolation of mouse CD8 T cells from spleens or lymph nodes utilizing a biotinylated antibody cocktail and streptavidin-coated magnetic beads. Undesired cells are bound by antibody and then magnetic beads that, when placed in a magnetic field, leave CD8 T cells untouched and free in solution.

The MagniSort™ Mouse CD8 T cell Enrichment Antibody Cocktail contains the following antibodies:
Anti-Mouse CD4 Biotin
Anti-Mouse CD11b Biotin
Anti-Mouse CD19 Biotin
Anti-Mouse CD24 Biotin
Anti-Mouse CD45R (B220) Biotin
Anti-Mouse CD49b (Integrin alpha 2) Biotin
Anti-Mouse Ly-6G (Gr-1) Biotin
Anti-Mouse γδ TCR Biotin
Anti-Mouse TER-119 Biotin

Reported Application
Magnetic Cell Separation

Dynabeads™ Sheep Anti-Rat IgG (Invitrogen™)

Dynabeads® Sheep Anti-Rat IgG are 4.5 µm superparamagnetic beads with affinity purified polyclonal sheep anti-rat IgG covalently bound to the bead surface. These beads can be used to isolate any cell from any species (except rat), depending on the specificity of the monoclonal rat IgG employed. Dynabeads® Sheep Anti-Rat IgG are especially well suited for cell isolation from mouse samples (cross-reactivity to mouse antibodies is high and cross-reactivity to human antibodies is minimal).

• Works with any cell type, from any sample and from any species (except rat)
• Fast and efficient depletion or positive isolation of any target cell for molecular downstream assays using rat primary antibody
• Obtain pure and untouched cells using a mixture of rat IgGs to deplete unwanted cells

Magnetic bead-based separation offers easy handling
The rat primary antibody may be added to the cell sample (indirect technique) or pre-coated onto the Dynabeads® Sheep Anti-Rat IgG (direct technique). Regardless of which of these techniques is used, when the cell sample and the Dynabeads® Sheep Anti-Rat IgG are mixed, the beads bind to the target cells. Placing the sample on a magnet separates the bead-bound cells from the rest of the sample. One or more rat primary antibodies may be used to either deplete the sample of unwanted cells (negative isolation) or to capture specific cells for molecular downstream experiments (positive isolation). For positive isolation of bead-free cells (e.g., for flow cytometry), we recommend using your rat antibody in combination with the Dynabeads® FlowComp™ Flexi Kit.

Useful for many sample types
Using Dynabeads® Sheep Anti-Rat IgG, you can isolate cells from many starting samples including whole blood, mouse spleen or lymph node cells, and tissue digests.

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MagniSort™ Mouse CD49b Positive Selection Kit (Invitrogen™)

The MagniSort™ Mouse CD49b Positive Selection Kit is designed for the magnetic separation of CD49b+ cells by positive selection. It has been optimized for the isolation of CD49b+ cells from mouse spleens or lymph nodes utilizing a biotinylated Anti-Mouse CD49b antibody and streptavidin-coated magnetic beads. CD49b+ cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the undesired cells can be separated from CD49b+ cells by decanting.

After positive selection, the purity of selected cells can be verified by staining with Anti-Mouse CD49b (integrin alpha 2), clone DX5, and Anti-Mouse NK1.1, clone PK136. For mouse strains that do not express NK1.1 antigen such as BALB/c, SJL, AKR, CBA C3H, Anti-Mouse CD335 (NKp46), clone 29A1.4, may be used.

Reported Application
Magnetic Cell Separation

MagniSort™ Mouse CD45 Depletion Kit (Invitrogen™)

The MagniSort™ Mouse CD45 Depletion Kit is designed for the magnetic depletion of mouse CD45+ cells from mouse spleens, bone marrow or digested lung tissue. It utilizes a biotinylated Anti-Mouse CD45 antibody and streptavidin-coated magnetic beads. CD45+ cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the CD45- cells can be separated from CD45+ cells by decanting.

After depletion, the purity of enriched CD45- cells can be verified by staining with Anti-Mouse CD45, clone 30-F11.

Reported Application
Magnetic Cell Separation

Dynabeads™ Mouse T-Activator CD3/CD28 for T-Cell Expansion and Activation (Gibco™)

Dynabeads® Mouse T-Activator CD3/CD28 are 4.5 µm superparamagnetic beads with anti-CD3 and anti-CD28 antibodies covalently bound to the bead surface. Dynabeads® Mouse T-Activator CD3/CD28 is designed for the activation and expansion of mouse T cells for research purposes.

• Activation of T cells without the need for feeder cells
• Activated cells that retain in vivo-like function
• Maximal reproducibility without contamination by soluble antibodies or mitogens
• Up to 1,000-fold T cell expansion in two weeks

Simple method for T cell activation
Dynabeads® Mouse T-Activator CD3/CD28 offers a simple method for activation and expansion of T cells that does not require feeder cells (antigen-presenting cells) or antigen. The 4.5 µm diameter, inert, superparamagnetic beads are similar in size to antigen-presenting cells, and are covalently coupled to anti-CD3 and anti-CD28 antibodies. These two antibodies provide primary and co-stimulatory signals, optimized for efficient T cell activation and expansion. Expansion of the T cell population can be stimulated using recombinant IL-2, and after activation or expansion, the magnetic beads can be easily removed using a DynaMag™ magnet.

Starting samples
Start with single-cell suspensions from spleen or lymph nodes, mononuclear cells (MNCs), whole blood, or T cell subsets including CD3+ T cells, CD4+ or CD8+ T cells, or regulatory T cells. Learn more about all of our Dynabeads® cell isolation products.

Downstream applications
The activated T cells can be analyzed shortly after activation (for transfection/transduction or to study TCR signaling, proteomics, gene expression, etc.). T cells can be left in culture to differentiate into T helper cell subsets or expansion of polyclonal/antigen-specific T cells.

Learn more about Dynabeads® products
• Find Dynabeads® products for a whole range of applications.
• Find magnets for Dynabeads® separations.

MagniSort™ Human CD4 T cell 2-Step Enrichment Kit (Invitrogen™)

The MagniSort™ Human CD4 2-Step Enrichment Kit is designed for the magnetic separation of CD4+ T cells by positive selection after first depleting monocytes. It has been optimized for the isolation of CD4+ T cells from human peripheral blood monocuclear cells, utilizing biotinylated Anti-Human CD14 and Anti-Human CD4 antibodies, as well as streptavidin-coated magnetic beads. In the first step, monocytes are bound by Anti-Human CD14 antibody and then magnetic beads. When placed in a magnetic field, the monocytes are held in the magnet while the remaining cells are decanted and used for the second step. In the second step, the monocyte-depleted cells are bound by Anti-Human CD4 antibody and then magnetic beads. Now, when placed in the magnetic field, the CD4+ T cells are held in the magnet while undesired cells can be decanted.

After enrichment, the purity of selected cells can be verified by staining with Anti-Human CD4, clones OKT4 or SK3.

Reported Application
Magnetic Cell Separation

Dynabeads™ CD14 (Invitrogen™)

Dynabeads® CD14 are superparamagnetic beads coupled with an anti-human CD14 antibody that enable easy isolation or depletion of human CD14+ cells directly from any sample, including whole blood, bone marrow, buffy coat, mononuclear cells (MNC), and tissue digests. Advantages of Dynabeads® CD14:

• Fast and efficient isolation of CD14+ cells from any sample—no columns required
• High purity, yield, and viability of positively isolated CD14+ cells for molecular downstream assays
• Fast and efficient depletion of CD14+ cells from any sample

About Dynabeads® CD14
Dynabeads® CD14 are uniform, superparamagnetic beads (4.5 µm diameter) coated with a primary monoclonal antibody specific for the CD14 membrane antigen mainly expressed mainly on human monocytes. Because of the bead size, Dynabeads® CD14 can easily and efficiently isolate or deplete cells from viscous samples such as whole blood and bone marrow in about 30 minutes. Dynabeads® CD14 will also afford excellent recovery of high-purity, viable cells for use in downstream molecular studies; for example, those in which cells are to be lysed while still attached to beads, and nucleic acids or proteins further purified. Note that intact cells will not be released from these beads, so if you want to isolate CD14+ cells for cell-based applications, or you need to check your samples with flow cytometry, check out our total human monocyte product offerings.

Magnetic bead–based separation offers easy handling
Dynabeads® CD14 is added to the sample under continuous mixing to optimize the binding of the Dynabeads® to the target cells. By placing the sample on a magnet, it separates the bead-bound target cells from the rest of the sample in just 1–2 minutes. For depletion, remove the supernatant to a new tube for further studies and discard the bead-bound cells. For positive isolation for molecular studies, remove the supernatant and wash the bead-bound cells 2–3 times in buffer to get optimal purity. The cells can be lysed while still attached to the beads and the supernatant transferred to a new tube for downstream molecular analysis. Starting samples can be whole blood, buffy coat, bone marrow, PBMC, or tissue digests.

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Dynabeads™ Mouse CD8 (Lyt 2) (Invitrogen™)

Dynabeads® Mouse CD8 (Lyt 2) are magnetic beads that provide a robust and versatile tool to isolate or deplete mouse CD8+ T-cells directly from any sample, including tissue digests such as spleen or lymph nodes, whole blood, bone marrow, and mononuclear cells (MNC).
• Rapid isolation of mouse CD8+ T-cells from any sample – no columns required
• Positive isolation for molecular downstream assays or efficient depletion of mouse CD8+ T cells

Excellent Yield, Purity, and Viability of Recovered Cells
Dynabeads® Mouse CD8 (Lyt 2) are uniform, superparamagnetic beads (4.5 µm diameter) that enable easy isolation of mouse CD8+ cells from spleen or lymph nodes, although any single-cell suspension may be used. Dynabeads® Mouse CD8 beads are coated with a primary monoclonal antibody specific for the Lyt 2 membrane antigen mainly expressed on cytotoxic/suppressor subpopulation of mature T-cells of all common mouse strains. Dynabeads® Mouse CD8 binds to CD8+ cells during a short incubation period in a tube, after which the bead-bound cells are separated from unbound cells using a magnet. This fast and gentle isolation method does not require the use of columns, and helps ensure high purity, recovery and viability of the isolated CD8+ cells.

Ideal for Both Depletion and Positive Isolation for Downstream Molecular Assays
Because of the powerful magnets used for separation, Dynabeads® Mouse CD8 (Lyt 2) can be used in viscous samples such as single cell suspensions from tissue, whole blood and bone marrow, and enable efficient depletion of mouse CD8+ cells in about 30 minutes. Dynabeads® Mouse CD8 (Lyt 2) will also afford excellent recovery of high-purity, viable cells when used for positive isolation for downstream studies; for example, those in which cells are to be lysed while still attached to beads, and nucleic acids or proteins further purified. Note that intact cells will not be released from these beads, so if you want to isolate mouse CD8+ cells for cell-based applications, or you need to check your samples with flow cytometry, you should use Dynabeads® FlowComp Mouse CD8 Cells (for bead-free cells).

Select what is right for you from our range of Mouse CD8 Isolation Products.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

Dynabeads™ Untouched™ Human CD4 T Cells Kit (Invitrogen™)

Use this kit to isolate pure and viable untouched CD4+ T-cells from PBMC by negative isolation. The kit depletes CD8+ T cells, B cells, NK cells, monocytes, platelets, dendritic cells, granulocytes and erythrocytes. The negatively isolated human CD4+ T-cells are left in the sample and have not been in contact with the Dynabeads. The bead and antibody-free CD4+ T-cells are truly untouched, and in perfect shape for any functional assay and application.

• High purity, recovery and viability of human untouched CD4+ T cells
• Easy to use and to scale up
• No columns required

Methodology: An antibody mix towards the non-CD4 cells is added to the sample and allowed to bind to the cells. Dynabeads® are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched human CD4+ T-cells can be directly analyzed in a flow cytometer and used in any application.

Note: A negative isolation strategy isolates pure and truly untouched cells. When maximum cell purity is critical, a positive cell isolation strategy is recommended

Starting Sample:
PBMC

MagniSort™ Human CD8 Naïve T cell Enrichment Kit (Invitrogen™)

The MagniSort™ Human CD8 Naïve T cell Enrichment Kit is designed for the magnetic separation of CD8 naïve T cells by negative selection. It has been optimized for the isolation of human CD8 naïve T cells from normal human peripheral blood mononuclear cells utilizing a biotinylated antibody cocktail and streptavidin-coated magnetic beads. Undesired cells are bound by antibody and then magnetic beads that, when placed in a magnetic field, leave CD8 naïve T cells untouched and free in solution.

The MagniSort™ Human CD8 Naïve T cell Enrichment Antibody Cocktail contains the following antibodies:
Anti-Human CD4 Biotin
Anti-Human CD11b Biotin
Anti-Human CD14 Biotin
Anti-Human CD16 Biotin
Anti-Human CD19 Biotin
Anti-Human CD20 Biotin
Anti-Human CD36 Biotin
Anti-Human CD45RO Biotin
Anti-Human CD56 Biotin
Anti-Human CD123 Biotin
Anti-Human CD235a Biotin
Anti-Human γδ TCR Biotin

Reported Application
Magnetic Cell Separation

Dynabeads™ Epithelial Enrich (Invitrogen™)

Enrich epithelial tumor cells directly from whole blood, bone marrow, or PBMC suspensions. Enriched tumor cells are lysed for mRNA isolation and RT-PCR amplification. The sensitivity of combined enrichment and RT-PCR detection can be as high as 1 tumor cell in 1 ml blood.

• Depletion of epithelial tumor cells from whole blood/bone marrow or PBMC.
• Positive isolation of epithelial tumor cells for downstream molecular analysis.
• No columns required

Starting samples:
Whole blood, bone marrow, PBMC, buffy coat.

Number of cells processed:
200 ml whole blood or ~4 × 109 PBMC.

MagniSort™ Mouse CD4 Naïve T cell Enrichment Kit (Invitrogen™)

The MagniSort™ Mouse CD4 Naïve T cell Enrichment Kit is designed for the magnetic separation of CD4 naïve T cells by negative selection. It has been optimized for the isolation of mouse CD4 naïve T cells from spleens or lymph nodes utilizing a biotinylated antibody cocktail and streptavidin-coated magnetic beads. Undesired cells are bound by antibody and then magnetic beads that, when placed in a magnetic field, leave CD4 naïve T cells untouched and free in solution.

The MagniSort™ Mouse CD4 Naïve T cell Enrichment Antibody Cocktail contains the following antibodies:
Anti-Mouse CD8 Biotin
Anti-Mouse CD11b Biotin
Anti-Mouse CD19 Biotin
Anti-Mouse CD24 Biotin
Anti-Mouse CD44 Biotin
Anti-Mouse CD45R (B220) Biotin
Anti-Mouse CD49b (Integrin alpha 2) Biotin
Anti-Mouse Ly-6G (Gr-1) Biotin
Anti-Mouse γδ TCR Biotin
Anti-Mouse TER-119 Biotin

Reported Application
Magnetic Cell Separation

MagniSort™ Mouse F4/80 Positive Selection Kit (Invitrogen™)

The MagniSort™ Mouse F4/80 Positive Selection Kit is designed for the magnetic separation of F4/80+ cells by positive selection. It has been optimized for the isolation of F4/80+ cells from mouse bone marrow or splenocytes utilizing a biotinylated Anti-Mouse F4/80 antibody and streptavidin-coated magnetic beads. F4/80+ cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the undesired cells can be separated from F4/80+ cells by decanting.

After positive selection, the purity of selected cells can be verified by staining with Anti-Mouse F4/80, clone BM8, and Anti-Mouse CD11b, clones M1/70.

Reported Application
Magnetic Cell Separation

MagniSort™ Mouse CD45.1 Positive Selection Kit (Invitrogen™)

The MagniSort™ Mouse CD45.1 Positive Selection Kit is designed for the magnetic separation of CD45.1+ cells by positive selection. It has been optimized for the isolation of CD45.1+ cells from mouse spleens or lymph nodes utilizing a biotinylated Anti-Mouse CD45.1 antibody and streptavidin-coated magnetic beads. CD45.1+ cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the undesired cells can be separated from CD45.1+ cells by decanting.

After positive selection, the purity of selected cells can be verified by staining with Anti-Mouse CD45.1, clone A20.

Reported Application
Magnetic Cell Separation

MagniSort™ Human B Cell Enrichment Kit (2nd Generation) (Invitrogen™)

The MagniSort™ Human B cell Enrichment Kit (2nd Generation) is designed for the magnetic separation of B cells by negative selection with improved purity. It has been optimized for the isolation of human B cells from normal human peripheral blood mononuclear cells utilizing a biotinylated antibody cocktail and streptavidin-coated magnetic beads. Undesired cells are bound by antibody and then magnetic beads that, when placed in a magnetic field, leave B cells untouched and free in solution.

The MagniSort™ Human B cell Enrichment Antibody Cocktail (2nd Generation) contains the following antibodies:
Anti-Human CD2 Biotin
Anti-Human CD3 Biotin
Anti-Human CD11b Biotin
Anti-Human CD14 Biotin
Anti-Human CD16 Biotin
Anti-Human CD36 Biotin
Anti-Human CD56 Biotin
Anti-Human CD235a Biotin

Reported Application
Magnetic Cell Separation

MagniSort™ Human CD8 Positive Selection Kit (Invitrogen™)

The MagniSort™ Human CD8 Positive Selection Kit is designed for the magnetic separation of CD8+ cells by positive selection. It has been optimized for the isolation of CD8+ cells from human peripheral blood monocuclear cells, utilizing a biotinylated Anti-Human CD8 antibody and streptavidin-coated magnetic beads. CD8+ cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the undesired cells can be separated from CD8+ cells by decanting.

After positive selection, the purity of selected cells can be verified by staining with Anti-Human CD8, clones RPA-T8 or SK1.

Reported Application
Magnetic Cell Separation

Dynabeads™ CD31 Endothelial Cell (Invitrogen™)

Isolate endothelial cells directly from tissue digests. Dynabeads® CD31 are ideal for positively isolating endothelial cells for downstream molecular analysis.

• Positively isolate endothelial cells directly from tissue digests for molecular applications
• Establish cultures for endothelial cells
• No columns required

Starting sample:
Tissue digests.