Shop All Magnetic Beads for Cell Isolation & Expansion

Dynabeads™ FlowComp™ Human CD14 Kit (Invitrogen™)

Dynabeads® FlowComp™ Human CD14 contain magnetic beads that provide a robust and versatile tool to positively isolate pure CD14+ monocytes from whole blood, bone marrow, buffy coat, mononuclear cells (MNC), or tissue digests. The cells can be released from the beads using the FlowComp™ Release Buffer.
• Rapid isolation of CD14+ monocytes from any sample – no columns required
• Pure and bead-free CD14+ monocytes for any downstream application

Excellent Yield, Purity, and Viability of Recovered Cells
Dynabeads® FlowComp™ Human CD14 contains uniform, superparamagnetic beads (2.8 µm diameter) that enable easy isolation of human CD14+ monocytes directly from any sample via a three-step process. First, the FlowComp™ Human CD14 Antibody is mixed with the sample in a tube where it binds to the CD14+ monocytes during a short incubation. Then, the CD14+ monocytes that have bound the specific antibodies are captured by the FlowComp™ Dynabeads® and separated from the unbound cells using a magnet. Finally, the CD14+ monocytes are released from the beads by adding the FlowComp™ Release buffer. This fast and gentle isolation method does not require the use of columns, and helps ensure high purity and viability of the isolated CD14+ monocytes.

Select what is right for you from our range of Human Monocyte Cell Isolation Products.

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

Dynabeads™ Mouse CD43 (Untouched™ B Cells) (Invitrogen™)

Use this kit to isolate pure and viable untouched B cells from mouse lymphoid organs by negative isolation. The kit depletes T cells, monocytes/macrophages, NK cells, plasma cells, CD5+ B1 cells, platelets, dendritic cells, granulocytes and erythrocytes. The negatively isolated mouse B cells are left in the sample and have not been in contact with the Dynabeads. The bead and antibody-free B cells are truly untouched, and in perfect shape for any functional assay and application.

High purity, recovery and viability of mouse untouched B cells

• Easy to use and to scale up
• No columns required

Methodology:
Dynabeads Mouse CD43 (Untouched B Cells) is added directly to the sample and binds the unwanted cells. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched mouse B cells can be directly analyzed in a flow cytometer and used in any application.

Starting Sample:
spleen or lymph nodes

Dynabeads™ CD45 (Invitrogen™)

Dynabeads® CD45 are superparamagnetic beads covalently coupled to anti-human CD45 antibody that enable isolation or depletion of CD45+ leucocytes directly from whole blood, buffy coat, or MNC suspensions. Dynabeads® CD45 can also be used to enrich epithelial tumor cells. The CD45 antigen is weakly expressed on myeloid cells. To efficiently deplete or isolate all leucocytes including myeloid cells, use Dynabeads® CD15 (Cat. No. 11137D) in combination with Dynabeads® CD45. Advantages of Dynabeads® CD45:

• Efficient depletion of human leucocytes
• Isolation of leucocytes directly from whole blood for molecular applications
• Enrichment of circulating epithelial tumor cells

About Dynabeads® CD45
Dynabeads® CD45 are uniform, superparamagnetic beads (4.5 µm diameter) coated with a primary monoclonal mouse IgG2a antibody specific for a CD45 membrane antigen common to all known isoforms of CD45. CD45 is expressed on all human leucocytes. Because of the bead size, Dynabeads® CD45 can easily and efficiently isolate or deplete cells from viscous samples such as whole blood and bone marrow in about 30 minutes. Dynabeads® CD45 is often used for enrichment of circulating non-hematopoietic tumor cells, by depletion of all CD45+ leucecytes from a MNC sample. Positively isolated cells can be used for downstream molecular studies; for example, those in which cells are to be lysed while still attached to beads, and nucleic acids or proteins further purified. Note that intact cells will not be released from these beads, and we do not recommend to analyze the bead-bound cells with a flow cytometer.

Magnetic bead–based separation offers easy handling
Dynabeads® CD45 is added to the sample under continuous mixing to optimize the binding of the Dynabeads® to the target cells. By placing the sample on a magnet it separates the bead-bound target cells from the rest of the sample in just 1–2 minutes. For depletion, remove the supernatant to a new tube for further studies and discard the bead-bound cells. For positive isolation for molecular studies, remove the supernatant and wash the bead-bound cells 2–3 times in buffer to get optimal purity. The cells can be lysed while still attached to the beads and the supernatant transferred to a new tube for downstream molecular analysis. Starting samples can be whole blood, buffy coat, bone marrow, PBMC, or tissue digests.

Learn more about Dynabeads® products
• Find Dynabeads® products for a whole range of applications.
• Find magnets for Dynabeads® separations.

MagniSort™ Mouse CD4 T cell Enrichment Kit (Invitrogen™)

The MagniSort™ Mouse CD4 T cell Enrichment Kit is designed for the magnetic separation of mouse CD4 T cells by negative selection. It has been optimized for the isolation of mouse CD4 T cells from spleens or lymph nodes utilizing a biotinylated antibody cocktail and streptavidin-coated magnetic beads. Undesired cells are bound by antibody and then magnetic beads that, when placed in a magnetic field, leave CD4 T cells untouched and free in solution.

The MagniSort™ Mouse CD4 T cell Enrichment Antibody Cocktail contains the following antibodies:
Anti-Mouse CD8 Biotin
Anti-Mouse CD11b Biotin
Anti-Mouse CD19 Biotin
Anti-Mouse CD24 Biotin
Anti-Mouse CD45R (B220) Biotin
Anti-Mouse CD49b (Integrin alpha 2) Biotin
Anti-Mouse Ly-6G (Gr-1) Biotin
Anti-Mouse γδ TCR Biotin
Anti-Mouse TER-119 Biotin

Reported Application
Magnetic Cell Separation

Dynabeads™ CD8 Positive Isolation Kit (Invitrogen™)

The Dynabeads® CD8 Positive Isolation Kit supplies superparamagnetic beads that enable gentle isolation of pure CD8+ T cells from whole blood, bone marrow, buffy coat, mononuclear cells (MNC), or tissue digests. The cells are released from the beads using the DETACHaBEAD® reagent to obtain bead- and antibody-free cells. Advantages of the Dynabeads® CD8 Positive Isolation Kit:

• Gentle isolation of CD8+ T cells from any sample—no columns required
• Bead- and antibody-free positive isolation of CD8+ cells for any downstream assay

About Dynabeads® CD8 Positive Isolation Kit
The Dynabeads® CD8 Positive Isolation Kit contains uniform, superparamagnetic beads (4.5 µm diameter) covalently coated with a monoclonal antibody specific for the CD8 membrane antigen predominantly expressed on the cytotoxic subset of human T cells. The kit also includes a DETACHaBEAD® reagent that releases the Dynabeads® from the purified CD8+ cells. This gently magnetic isolation process gives highly viable bead- and antibody-free cells to be used in any downstream assay.

Magnetic bead–based separation offers easy handling
Dynabeads® are mixed with the sample in a tube where they bind to the target cells during a short incubation, after which the bead-bound cells are separated from unbound cells using a magnet. The positively isolated cells are then washed, and DETACHaBEAD® reagent is added to gently release the cells from the beads. This magnetic bead-based positive isolation method gives both bead- and antibody-free cells. The isolated CD8+ cells are phenotypically unaltered and suitable for any downstream application. This fast and gentle isolation method does not require the use of columns, and helps ensure high purity and viability of the isolated CD8+ cells. For depletion or isolation of untouched CD8+ cells, select from our range of Human CD8+ T Cell Isolation Products.

Learn more about Dynabeads® products
• Find Dynabeads® products for a whole range of applications.
• Find magnets for Dynabeads® separations.

Dynabeads™ FlowComp™ Human CD3 Kit (Invitrogen™)

Dynabeads® FlowComp™ Human CD3 contain magnetic beads that provide a robust and versatile tool to positively isolate pure CD3+ T-cells from whole blood, bone marrow, buffy coat, mononuclear cells (MNC), or tissue digests. The cells can be released from the beads using the FlowComp™ Release Buffer.
• Rapid isolation of CD3+ T-cells from any sample – no columns required
• Pure and bead-free CD3+ T-cells for any downstream application

Excellent Yield, Purity, and Viability of Recovered Cells
Dynabeads® FlowComp™ Human CD3 contains uniform, superparamagnetic beads (2.8 µm diameter) that enable easy isolation of human CD3+ cells directly from any sample via a three-step process. First, the FlowComp™ Human CD3 Antibody is mixed with the sample in a tube where it binds to the target cells during a short incubation. Then, the CD3+ T-cells that have bound the specific antibodies are captured by the FlowComp™ Dynabeads® and separated from unbound cells using a magnet. Finally, the target cells are released from the beads by adding the FlowComp™ Release buffer. This fast and gentle isolation method does not require the use of columns, and helps ensure high purity, recovery and viability of the isolated CD3+ cells. The cells can be used in any downstream application.

Select what is right for you from our range of Human T Cell Isolation Products.

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

MagniSort™ Human CD8 T cell Enrichment Kit (Invitrogen™)

The MagniSort™ Human CD8 T cell Enrichment Kit is designed for the magnetic separation of CD8 T cells by negative selection. It has been optimized for the isolation of human CD8 T cells from normal human peripheral blood mononuclear cells utilizing a biotinylated antibody cocktail and streptavidin-coated magnetic beads. Undesired cells are bound by antibody and then magnetic beads that, when placed in a magnetic field, leave CD8 T cells untouched and free in solution.

The MagniSort™ Human CD8 T cell Enrichment Antibody Cocktail contains the following antibodies:
Anti-Human CD4 Biotin
Anti-Human CD11b Biotin
Anti-Human CD14 Biotin
Anti-Human CD16 Biotin
Anti-Human CD19 Biotin
Anti-Human CD20 Biotin
Anti-Human CD36 Biotin
Anti-Human CD56 Biotin
Anti-Human CD123 Biotin
Anti-Human CD235a Biotin
Anti-Human γδ TCR Biotin

Reported Application
Magnetic Cell Separation

MagniSort™ Human CD56 Positive Selection Kit (Invitrogen™)

The MagniSort™ Human CD56 Positive Selection Kit is designed for the magnetic separation of CD56+ cells by positive selection. It has been optimized for the isolation of CD56+ cells from human normal peripheral blood mononuclear cells utilizing a biotinylated Anti-human CD56 antibody and streptavidin-coated magnetic beads. CD56+ cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the undesired cells can be separated from CD56+ cells by decanting.

After positive selection, the purity of selected cells can be verified by staining with Anti-Human CD56 (clone CMSSB).

Reported Application
Magnetic Cell Separation

MagniSort™ Human CD4 Naïve T cell Enrichment Kit (Invitrogen™)

The MagniSort™ Human CD4 Naïve T cell Enrichment Kit is designed for the magnetic separation of CD4 naïve T cells by negative selection. It has been optimized for the isolation of human CD4 naïve T cells from normal human peripheral blood mononuclear cells utilizing a biotinylated antibody cocktail and streptavidin-coated magnetic beads. Undesired cells are bound by antibody and then magnetic beads that, when placed in a magnetic field, leave CD4 naïve T cells untouched and free in solution.

The MagniSort™ Human CD4 Naïve T cell Enrichment Antibody Cocktail contains the following antibodies:
Anti-Human CD8 Biotin
Anti-Human CD11b Biotin
Anti-Human CD14 Biotin
Anti-Human CD16 Biotin
Anti-Human CD19 Biotin
Anti-Human CD20 Biotin
Anti-Human CD36 Biotin
Anti-Human CD45RO Biotin
Anti-Human CD56 Biotin
Anti-Human CD123 Biotin
Anti-Human CD235a Biotin
Anti-Human γδ TCR Biotin

Reported Application
Magnetic Cell Separation

MagniSort™ Mouse CD3 Positive Selection Kit (Invitrogen™)

The MagniSort™ Mouse CD3 Positive Selection Kit is designed for the magnetic separation of CD3+ cells by positive selection. It has been optimized for the isolation of CD3+ cells from mouse spleens or lymph nodes utilizing a biotinylated Anti-Mouse CD3 antibody and streptavidin-coated magnetic beads. CD3+ cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the undesired cells can be separated from CD3+ cells by decanting.

After positive selection, the purity of selected cells can be verified by staining with Anti-Mouse CD3e, clone 145-2C11.

Reported Application
Magnetic Cell Separation

MagniSort™ Human CD117 (c-Kit) Positive Selection Kit (Invitrogen™)

The MagniSort™ Human CD117 (c-Kit) Positive Selection Kit is designed for the magnetic separation of CD117+ cells by positive selection. It has been optimized for the isolation of CD117+ cells from normal human peripheral blood cells. CD117+ cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the undesired cells can be separated from CD117+ cells by decanting.

After positive selection, the purity of selected cells can be verified by staining with Anti-Human CD117, clone YB5.B8, and Anti-Human CD34, clone 4H11.

Reported Application
Magnetic Cell Separation

MagniSort™ Human CD8 Naïve T cell Enrichment Kit (Invitrogen™)

The MagniSort™ Human CD8 Naïve T cell Enrichment Kit is designed for the magnetic separation of CD8 naïve T cells by negative selection. It has been optimized for the isolation of human CD8 naïve T cells from normal human peripheral blood mononuclear cells utilizing a biotinylated antibody cocktail and streptavidin-coated magnetic beads. Undesired cells are bound by antibody and then magnetic beads that, when placed in a magnetic field, leave CD8 naïve T cells untouched and free in solution.

The MagniSort™ Human CD8 Naïve T cell Enrichment Antibody Cocktail contains the following antibodies:
Anti-Human CD4 Biotin
Anti-Human CD11b Biotin
Anti-Human CD14 Biotin
Anti-Human CD16 Biotin
Anti-Human CD19 Biotin
Anti-Human CD20 Biotin
Anti-Human CD36 Biotin
Anti-Human CD45RO Biotin
Anti-Human CD56 Biotin
Anti-Human CD123 Biotin
Anti-Human CD235a Biotin
Anti-Human γδ TCR Biotin

Reported Application
Magnetic Cell Separation

CELLection™ Pan Mouse IgG Kit (Invitrogen™)

Use this kit with any mouse IgG for the positive isolation of a cell population from all species except mouse. Antibodies are attached to the surface of the CELLection™ Dynabeads® via a DNA linker; this linker provides a cleavable site to release and remove the beads from the cells after isolation. Bead-free cells are viable and can be used in any downstream application.

This Kit Contains: CELLection™ Dynabeads® coated with a human anti-mouse IgG monoclonal, and DNase Releasing Buffer

Applications:

• Positively isolate and detach any cell from any species (except mouse) using your own mouse IgG
• Compatible with flow cytometry, cell culture and all cell based assays

Dynabeads™ CD15 (Invitrogen™)

Dynabeads® CD15 are superparamagnetic beads covalently coupled with an anti-human CD15 antibody that efficiently depletes human CD15+ myeloid cells (predominantly neutrophil and eosinophil granulocytes) directly from whole blood, buffy coat, or PBMC samples. Dynabeads® CD15 can also be used to positively isolate CD15+ cells for molecular applications. Advantages of Dynabeads® CD15:

• Efficient depletion of granulocytes (neutrophils and eosinophils)
• Isolation of granulocytes directly from whole blood for molecular applications
• No columns required

About Dynabeads® CD15
Dynabeads® CD15 are uniform, superparamagnetic polystyrene beads (4.5 µm diameter) coated with a monoclonal mouse IgM antibody recognizing the human CD15 membrane antigen. The CD15 antigen is predominantly expressed on human neutrophil and eosinophil granulocytes, and to a varying degree on monocytes.

Magnetic bead-;based separation offers easy handling
Dynabeads® CD15 is added to the sample under continuous mixing to optimize the binding of the Dynabeads® to the target cells. By placing the sample on a magnet, it separates the bead-bound target cells from the rest of the sample in just 1–2 minutes. For depletion, remove the supernatant to a new tube for further studies and discard the bead-bound cells. For positive isolation for molecular studies, remove the supernatant and wash the bead-bound cells 2–3 times in buffer to get optimal purity. The cells can be lysed while still attached to the beads and the supernatant transferred to a new tube for downstream molecular analysis. Starting samples can be whole blood, PBMC, or buffy coat.

Learn more about Dynabeads® products
• Find Dynabeads® products for a whole range of applications.
• Find magnets for Dynabeads® separations.

MagniSort™ Mouse CD8 Positive Selection Kit (Invitrogen™)

The MagniSort™ Mouse CD8 Positive Selection Kit is designed for the magnetic separation of CD8+ cells by positive selection. It has been optimized for the isolation of CD8+ cells from mouse spleens or lymph nodes utilizing a biotinylated Anti-Mouse CD8a antibody and streptavidin-coated magnetic beads. CD8+ cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the undesired cells can be separated from CD8+ cells by decanting.

After positive selection, the purity of selected cells can be verified by staining with Anti-Mouse CD8a, clone 53-6.7.

Reported Application
Magnetic Cell Separation