Shop All Magnetic Beads Ligand-Coupled

Pierce™ Anti-HA Magnetic Beads (Thermo Scientific™)

Thermo Scientific Pierce Anti-HA Magnetic Beads are affinity particles for immunoprecipitation of recombinant HA-tagged proteins expressed in bacterial or mammalian cells or in vitro systems, using manual or robotic magnetic separators.

Features of Anti-HA Magnetic Beads:

Specific—highly specific anti-HA monoclonal antibody (clone 2-2.2.14) enables high yield and high purity immunoprecipitation
Convenient and fast—product instructions provide an easy-to-follow, optimized protocol for immunoprecipitation in approximately one hour
Low non-specific binding—stable, pre-blocked beads and specific antibody minimize off-target binding for HA-tag IP or co-IP experiments
Versatile—beads are compatible with manual and automated workflows (e.g., Thermo Scientific KingFisher Instruments)

The blocked magnetic bead surface is coated with anti-HA antibody, a highly specific mouse IgG1 monoclonal antibody that recognizes the HA-epitope tag (YPYDVPDYA) derived from the human influenza hemagglutinin (HA) protein. The Pierce Anti-HA Magnetic Beads can be used manually with a magnetic stand, as well as with automated platforms, such as the Thermo Scientific KingFisher Instruments, for high-throughput workflows.

Specifications:
Product Details:
Pierce Anti-HA Magnetic Beads are convenient for the immunoprecipitation (IP) of recombinant HA-tagged proteins and the co-immunoprecipitation (co-IP) of their interacting proteins. The beads are incubated with a cell lysate containing HA-tagged protein and the fusion protein is captured. The beads are subsequently washed and then the target proteins are eluted using 0.1M glycine, pH 2.0, 50 mM NaOH, or SDS-PAGE sample buffer. If gentler elution conditions are desired, 2 mg/mL Pierce HA peptide can be used. The protocol has been optimized for each of these conditions. Anti-HA antibody can be used to detect HA-tagged protein by Western blot analysis.

Pierce™ Protein A Magnetic Beads (Thermo Scientific™)

Thermo Scientific Pierce Protein A Magnetic Beads are high-capacity and high-throughput affinity particles for antibody purification and immunoprecipitation methods using manual or robotic magnetic separators.

Features of Protein A Magnetic Beads:

High efficiency – equivalent or higher yield of IP target antigens than magnetic beads from other suppliers
Low non-specific binding – stable, pre-blocked beads provide highly purified product (e.g., antigen eluted in IP with antibody is devoid of contaminating protein from complex cell lysate)
Consistent – magnetic beads eliminate resin loss and provide for more efficient separation than traditional IP methods that use only centrifugation
Versatile – beads are compatible with manual and automated workflows (e.g., Thermo Scientific KingFisher Instruments)

Recombinant protein A, covalently attached to a blocked magnetic bead surface, can bind to antibodies from many different species, enabling purification of antibodies from crude extracts. Immunoprecipitation assays performed with protein A coated beads result in high yield of target antigen with very low background. Using our crosslinker chemistry, you can immobilize an antibody onto the magnetic particle and prevent IgG contamination in IP or Co-IP experiments. The Pierce Protein A Magnetic Beads can be used manually with a magnetic stand, as well as with automated platforms, such as the Thermo Scientific KingFisher Instruments, for high-throughput workflows.

Applications:
• IP and Co-IP experiments
• Antibody purification

Thermo Scientific Pierce Protein A Magnetic Beads are used for purifying antibody from serum, cell culture supernatant or ascites, as well as for IP/Co-IP of antigens from cell or tissue extracts. Protein A can bind to antibodies from many different species, including mouse, human, rabbit, pig, dog, and cat. The protocol for the Pierce Protein A beads has been optimized to allow for high recovery and high purity of the isolated antibody or antigen. Antibody or antigen/antibody complex (IP) is first captured on the magnetic beads. The beads are washed and then the target is eluted with low pH elution buffer. IP performance is equivalent to or better than Protein A magnetic beads from other suppliers.

Dynabeads™ M-270 Streptavidin (Invitrogen™)

Dynabeads® M-270 Streptavidin the gold standard for isolation and handling of biotinylated nucleic acids, antibodies, or other biotinylated ligands and targets. The very high binding affinity of the streptavidin-biotin interaction (Kd=10-15) is used in a vast number of applications. Benefits and features:

• Direct and fast isolation of any biotinylated molecule
• Flexible protocols with gentle and efficient liquid-phase reaction kinetics
• Very low nonspecific binding of nucleotides and nucleic acids
• Very low aggregation in high-salt hybridization buffers
• Well suited for nucleic acid applications with extreme demands
• Low nonspecific binding of small and negatively charged proteins
• Production follows a validated process in compliance with cGMP for medical devices
• Well suited for automated protocols

About Dynabeads® M-270 Streptavidin
These uniform and superparamagnetic beads are 2.8 µm in diameter, with a monolayer, not a multilayer, of recombinant streptavidin covalently coupled to the surface. This leaves the vast majority of the biotin binding sites sterically available for binding, not only of free biotin, but also for binding of biotinylated ligands/targets. They are hydrophilic, negatively charged and show rapid liquid-phase reaction kinetics. Their specific and defined surface allow for efficient capture, separation, and downstream handling. The streptavidin monolayer ensures negligible leakage, and the lack of excess adsorbed streptavidin ensures batch consistency and reproducibility of your results. Applications
Over the past 15 years, streptavidin-coupled Dynabeads® have been used and cited for a very wide variety of applications. Examples include direct/indirect isolation and downstream handling of nucleic acids, proteins/peptides, and other target molecules. Ideal for sequence-specific DNA/RNA capture in nucleic acid–based diagnostics, specifically with samples with a high chaotropic salt concentration, immunoassays involving small biotinylated antigens, and applications that are not compatible with BSA (these beads are not blocked with BSA). Easily adaptated to automated processes. Dynabeads® are used on more than 25,000 routine IVD instruments worldwide. The product holds high standards with respect to reproducibility (both within and between batches), and automation ability, and drives reliability for your results.

Binding capacity
The size of the molecule and the biotinylation procedure will affect the binding capacity. The capacity also depends on steric availability and charge interaction between bead and molecule and between molecules. There are two or three biotin binding sites available for each streptavidin molecule on the surface of the bead after immobilization. One mg of Dynabeads® M-270 Streptavidin typically binds:

• >950 pmoles free biotin
• ~200 pmol biotinylated peptides
• ~10 µg biotinylated IgG
• ~10 µg ds-DNA
• ~200 pmol ss-oligonucleotides

Dynabeads™ Oligo(dT)25 (Invitrogen™)

Dynabeads® Oligo(dT)25 mRNA isolation beads specifically target and capture mRNA molecules from virtually any crude sample and eliminate the need to purify total RNA when the desired information-bearing nucleic acid is mRNA. Since mRNA comprises only about 1–5% ot total cellular RNA, the isolation of total RNA is not the most efficient way to isolate mRNA. Other technologies designed to purify total RNA yield ~80% ribosomal RNA and force mRNA to compete with ribosomal RNA, transfer RNA, micro RNA, small nucleolar RNA, and small cytoplasmic RNA for membrane binding. Advantages of Dynabeads® Oligo(dT)25 beads:

• Fast and gentle procedure yields pure intact mRNA
• Extremely pure mRNA isolation, best choice upstream of cDNA synthesis
• Exquisitely sensitive mRNA isolation enables cDNA synthesis and cDNA library construction from ultra-small starting samples (enables cDNA library construction from a single cell)

How the beads work
The oligo(dT)25-coated Dynabeads® specifically target and capture the mRNA transcriptome from an extremely wide variety of crude starting samples. Ribosomal RNA, DNA, proteins, and small RNA molecules (such as transfer RNA, micro RNA, and small nucleolar RNA) do not bind to the beads and are discarded. Only polyadenylated RNA species (mRNA) are captured. Isolated mRNA is pure, eliminating the need for ribosomal RNA subtraction or a post-extraction DNase treatment. This column-free system ensures the highest transcriptome recovery:

• Physical mRNA capture on mobile magnetic beads
• Rapid and gentle magnetic handling procedures
• No mRNA lost during high g-force spins
• No mRNA trapped in column membranes during elution

Applications
mRNA is suitable for all downstream molecular applications, including gene cloning, cDNA synthesis, cDNA library construction, RT-PCR, quantitative RT-PCR, RPA (Ribonuclease Protection Assay), subtractive hybridization, primer extension, SAGE, RACE, and others. The Dynabeads® Oligo(dT)25 mRNA isolation beads are the ideal mRNA purification method prior to cDNA library construction. Use of these beads ensures the highest recovery and enrichment of the transcriptome. These beads capture more of the transcriptome than is possible with methods that integrate a total RNA isolation step upstream of mRNA isolation.

Verastile elution options
Elution can be performed in any volume down to 5 µL. mRNA elution is optional because enzymatic reactions in downstream procedures are not inhibited by presence of Dynabeads®. Additionally, one can perform cDNA synthesis directly on the beads to create a reusable solid-phase cDNA library.

Pierce™ Anti-c-Myc Magnetic Beads (Thermo Scientific™)

Thermo Scientific Pierce Anti-c-Myc Magnetic Beads are affinity particles for immunoprecipitation of recombinant c-Myc-tagged proteins expressed in bacterial or mammalian cells or in vitro systems, using manual or robotic magnetic separators.

Features of Anti-c-Myc Magnetic Beads:

Specific—highly specific anti-c-Myc monoclonal antibody (clone 9E10) enables high yield and high purity immunoprecipitation
Convenient and fast—product instructions provide an easy-to-follow, optimized protocol for immunoprecipitation in approximately one hour
Low non-specific binding—stable, pre-blocked beads and specific antibody minimize off-target binding for c-Myc-tag IP or co-IP experiments
Versatile—beads are compatible with manual and automated workflows (e.g., Thermo Scientific KingFisher Instruments)

The blocked magnetic bead surface is coated with anti-c-Myc antibody, a highly specific mouse IgG1 monoclonal antibody (clone 9E10) that recognizes the c-Myc-epitope tag (EQKLISEEDL) derived from the human c-myc oncogene (p62 c-myc). The Pierce Anti-c-Myc Magnetic Beads can be used manually with a magnetic stand, as well as with automated platforms, such as the Thermo Scientific KingFisher Instruments, for high-throughput workflows.

Specifications:
Product Details:
Pierce Anti-c-Myc Magnetic Beads are convenient for the immunoprecipitation (IP) of recombinant c-Myc tagged proteins and the co-immunoprecipitation (Co-IP) of their interacting proteins. The beads are incubated with a cell lysate containing c-Myc tagged protein and the fusion protein is captured. The beads are subsequently washed and then the target proteins are eluted using 0.1M glycine (pH 2.0) 50 mM NaOH, or SDS-PAGE sample buffer, the protocol has been optimized for each of these conditions. Anti-c-Myc antibody can be used to detect c-Myc tagged protein by Western blot analysis.

Dynabeads™ Protein A and Magnet Starter Pack (Invitrogen™)

The Dynabeads Protein A and Magnet Starter Pack combines Dynabeads Protein A magnetic beads for immunoprecipitation with the magnet preferred for use with 1.5 mL microcentrifuge tubes for purchasing convenience. The beads are sufficient for 40 reactions, and the magnet holds 16 x 1.5 mL microcentrifuge tubes. Dynabeads Protein A beads and the DynaMag-2 magnet are also available separately.

The product contains:
2 x 1 mL Dynabeads Protein A beads
1 x DynaMag-2 magnet

Pierce™ Protein G Magnetic Beads (Thermo Scientific™)

Thermo Scientific Pierce Protein G Magnetic Beads are high-capacity and high-throughput affinity particles for antibody purification and immunoprecipitation methods using manual or robotic magnetic separators.

Features of Protein G Magnetic Beads:

High efficiency – equivalent or higher yield of IP target antigens than magnetic beads from other suppliers
Low non-specific binding – stable, pre-blocked beads provide highly purified product (e.g., antigen eluted in IP with antibody is devoid of contaminating protein from complex cell lysate)
Consistent – magnetic beads eliminate resin loss and provide for more efficient separation of solutions than traditional IP methods that use only centrifugation
Versatile – beads are compatible with manual and automated workflows (e.g., Thermo Scientific KingFisher Instruments)

Recombinant protein G, covalently attached to a blocked magnetic bead surface, can bind to antibodies from many different species, enabling purification of antibodies from crude extracts. Immunoprecipitation assays performed with protein G coated beads result in high yield of target antigen with very low background. Using our crosslinker chemistry, you can immobilize an antibody onto the magnetic particle and prevent IgG contamination in IP or Co-IP experiments. The Pierce Protein G Magnetic Beads can be used both manually with a magnetic stand as well as with automated platforms such as the Thermo Scientific KingFisher Instruments for high-throughput workflows.

Applications:
• IP and Co-IP experiments
• Antibody purification

Thermo Scientific Pierce Protein G Magnetic Beads are used for purifying antibody from serum, cell culture supernatant or ascites as well as for IP/Co-IP of antigens from cell or tissue extracts. Protein G can bind to antibodies from many different species, including mouse, human, rabbit, cow, goat and sheep. The protocol for the Pierce Protein G beads has been optimized to allow for high recovery and high purity of the isolated antibody or antigen. Antibody or antigen/antibody complex (IP) is first captured on the magnetic beads. The beads are washed and then the target is eluted with low pH elution buffer. IP performance is equivalent to or better than Protein G magnetic beads from other suppliers.

Pierce™ Protein A/G Magnetic Beads (Thermo Scientific™)

Thermo Scientific Pierce Protein A/G Magnetic Beads are high-performance affinity particles for antibody purification and immunoprecipitation methods using manual or robotic magnetic separators.

Features of Protein A/G Magnetic Beads:

High capacity—nearly four times higher binding capacity than typical magnetic beads from other suppliers, allowing the use of smaller amounts per experiment
Low non-specific binding—stable, pre-blocked beads provide clean purification products (e.g., antigen eluted in IP with antibody is devoid of contaminating proteins from complex IP matrix)
Flexibility—convenience of IgG binding domains of both Protein A and Protein G on one bead
Compatibility—beads are compatible with manual and automated applications (e.g., Thermo Scientific KingFisher Instruments)
Assay consistency—magnetic beads eliminate resin loss and provide for more efficient separation of solutions than traditional IP methods that use only microcentrifuge tubes

These magnetic beads are coated with genetically engineered Pierce Protein A/G, a recombinant fusion protein which combines the IgG binding domains of both Protein A and Protein G. This enables capture of antibodies from a wider range of species and isotypes than either protein alone. Using our crosslinker chemistry, you can immobilize an antibody onto the magnetic particle and prevent IgG contamination in your immunoprecipitated sample. These beads can be used both manually with a magnetic stand as well as with automated platforms such as the Thermo Scientific KingFisher Instruments.

Applications:
• IP and Co-IP experiments (see complete kit)
• Immunoprecipitation for analysis in non-reducing conditions
• Antibody purification

The recombinant Protein A/G that is immobilized onto the Pierce Magnetic Beads is a fusion of the IgG binding domains of both Protein A and Protein G. Protein A/G contains four Fc-binding domains from Protein A and two from Protein G, making it a convenient tool for investigating and purifying immunoglobulins. Thus, Pierce Magnetic Particles are not simply a mixed immobilization of separate Protein A and Protein G polypeptides, nor are they a mixture of Protein A magnetic beads and Protein G magnetic beads.

Dynabeads™ M-280 Streptavidin (Invitrogen™)

Dynabeads® M-280 Streptavidin are the gold standard for the isolation and handling of biotinylated nucleic acids, antibodies, or other biotinylated ligands and targets. The very high binding affinity of the streptavidin-biotin interaction (Kd=10-15) is used in a vast number of applications. Benefits and features:

• Direct and fast isolation of any biotinylated molecule
• Low-charged and neutral beads, optimal for binding of DNA fragments, proteins, peptides, and antibodies
• Flexible protocols with gentle and efficient liquid-phase reaction kinetics
• Biomagnetic protocols are easily adapted to automated platforms
• High batch-to-batch reproducibility, securing consistent results in your application

About Dynabeads® M-280 Streptavidin
These uniform and superparamagnetic beads are 2.8 µm in diameter, with a monolayer, not a multilayer, of recombinant streptavidin covalently coupled to the surface and further blocked with BSA. The monolayer of streptavidin leaves the vast majority of the biotin binding sites sterically available for binding, not only of free biotin, but also for binding of biotinylated ligands/targets. They show rapid liquid-phase reaction kinetics. Their specific and defined surface allow for efficient capture, separation, and downstream handling. The streptavidin monolayer ensures negligible leakage, and the lack of excess adsorbed streptavidin ensures batch consistency and reproducibility of your results.Applications
Over the past 15 years, Dynabeads® M-280 Streptavidin have been used and cited for a very wide variety of applications. Key applications include preparing 2–5 kb samples for mate pair library sequencing using Illumina platforms preparing single-stranded DNA templates, isolation of RNA and DNA binding proteins, immobilization of large DNA fragments, purifying sequencing products, and the specific capture of nucleic acids. Dynabeads® are used on more than 25,000 routine IVD instruments worldwide. The product holds high standards with respect to reproducibility (both within and between batches), and automation ability, and drives reliability for your results.

Binding capacity
The size of the molecule and the biotinylation procedure will affect the binding capacity. The capacity also depends on steric availability and charge interaction between bead and molecule and between molecules. There are two or three biotin binding sites available for each streptavidin molecule on the surface of the bead after immobilization. One mg of Dynabeads® M-280 Streptavidin typically binds:

• 650–900 pmoles free biotin
• ~200 pmol biotinylated peptides
• ~10 µg biotinylated IgG
• ~10 µg ds-DNA
• ~200 pmol ss-oligonucleotides

MagnaBind™ Streptavidin Beads (Thermo Scientific™)

Thermo Scientific MagnaBind Streptavidin Beads are convenient for affinity purification or separation methods involving of biotin-labeled molecules. To remove the MagnaBind Beads from the suspension, an external magnetic field is used.

Features of MagnaBind Streptavidin Beads:

Composition: Silanized iron oxide
Magnetization: 25-35EMU/g
Type of Magnetization: Superparamagnetic (no magnetic memory)
Surface Area: >100m2/g
Bead Size: 1-4µm diameter
Settling Rate: 4% in 30 minutes
Effective Density: 2.5g/mL
Number of Beads: 1 × 108 beads/mg
pH Stability: Aqueous solution, above pH 4.0
Concentration: 5 mg/mL

Streptavidin is a 60kDa protein from Streptomycetes avidinii. The protein is a tetramer having four biotin-binding sites. Unlike avidin, streptvidin has a low isoelectric point (pI=5) and no carbohydrate groups, resulting in low nonspecific binding. The high-affinity interaction between streptavidin and biotin cannot be dissociated efficiently except with very harsh conditions, such as boiling in sample loading buffer for SDS-PAGE or 8M guanidine• HCl, pH 1.5. Consequently, it is often possible to elute binding partners in an interaction complex without also eluting the biotinylated component.

Applications:
• Cell sorting using either positive or negative selection
• Protein purification or immunoassays using either direct or indirect methods

MagnaBind™ Goat Anti-Rabbit IgG (Thermo Scientific™)

Thermo Scientific™ MagnaBind™ Beads provide a convenient method for magnetic separation of antibodies, antigens, lectins, enzymes, nucleic acids and cells using affinity binding. To remove the MagnaBind Beads from the suspension, an external magnetic field is used.

MagnaBind Goat Anti-Rabbit IgG Beads cab be used to conveniently separate cells of interest from a cell mixture. The monoclonal or polyclonal antibody to the cell surface antigen is pre-incubated with the appropriate magnetic bead that is then incubated with the cell suspension.

Features of MagnaBind Goat Anti-Rabbit IgG Beads:

Composition: Silanized iron oxide
Magnetization: 25-35EMU/g
Type of Magnetization: Superparamagnetic (no magnetic memory)
Surface Area: >100m2/g
Bead Size: 1-4µm diameter
Settling Rate: 4% in 30 minutes
Effective Density: 2.5g/mL
Number of Beads: 1 × 108 beads/mg
pH Stability: Aqueous solution, above pH 4.0
Concentration: ~1 mg/mL

Applications:
• Cell sorting using either positive or negative selection
• Protein purification or immunoassays using either direct or indirect methods

Pierce™ Protein L Magnetic Beads (Thermo Scientific™)

Thermo Scientific Pierce Protein L Magnetic Beads are high-capacity and high-throughput affinity particles for antibody purification using manual or robotic magnetic separators.

Features of Protein L Magnetic Beads:

Selective—immobilized Protein L is ideal for selective purification of human and mouse antibodies that have kappa light chains
Low non-specific binding—stable, pre-blocked beads provide clean purification of antibody
Versatile—beads are compatible with manual and automated workflows (e.g., Thermo Scientific KingFisher Instruments)

Recombinant Protein L, covalently attached to a blocked magnetic bead surface, selectively binds mouse and human antibodies through kappa light chains. The beads are commonly used to purify monoclonal antibodies in cell culture supernatants supplemented with bovine serum as Protein L does not bind bovine IgG. The Pierce Protein L Magnetic Beads can be used manually with a magnetic stand, as well as with automated platforms such as the Thermo Scientific KingFisher Instruments for high-throughput workflows.

Applications:
• Purification of monoclonal and polyclonal antibodies that bind poorly to Protein A or Protein G magnetic beads
• Purification of monoclonal antibodies from culture supernatants supplemented with bovine serum
• Protein L IP and co-IP assays
• Purification of ScFv and Fab fragments containing kappa light chains

Thermo Scientific Pierce Protein L Magnetic Beads are typically used for purifying mouse and human antibodies containing kappa light chains from serum, cell culture supernatant or ascites. Protein L can bind a broader range of Ig classes than Protein A or Protein G, including IgG, IgM, IgA, IgE and IgD. Protein L binds strongly to human (kappa I, III and IV only), mouse (kappa I only), rat and pig immunoglobulins. It binds weakly to rabbit immunoglobulins and does not bind bovine, goat or sheep immunoglobulins. Single chain variable fragments (scFv) and Fab fragments also bind to Protein L. The protocol for the Pierce Protein L beads has been optimized to allow for high recovery and high purity of isolated antibodies.

Dynabeads™ Protein G Immunoprecipitation Kit (Invitrogen™)

The Dynabeads™ Protein G Immunoprecipitation Kit is a faster and easier solution for immunoprecipitation (IP) than using Sepharose™ resin or agarose resin, and includes all reagents and buffers required to perform IP using your own antibody. The kit is optimized for standard IP, but can also be used for Co-IP, chromatin IP (ChIP), or RNA IP (RIP). The kit includes the widely used Dynabeads Protein G with recombinant Protein G (~17 kDa) covalently coupled to the magnetic bead surface. Both manual and automated protocols are available.

• IP in less than 40 minutes
• High target protein yield with low antibody consumption
• Very low non-specific binding with high signal-to-noise ratio
• No columns, centrifugations, or time-consuming pre-clearing required
• High reproducibility with Dynabeads Protein G and all buffers supplied in the kit
• High throughput compatible with KingFisher™ instruments

Manual Dynabeads separation is fast and easy to perform
The manual protocol is simple and can be performed in under 40 minutes. First, the antibody for the target protein is incubated with the Dynabeads Protein G in a tube for 10 minutes. Excess antibody is washed away by placing the tube in a DynaMag™ magnet and removing the supernatant. The antibody-coated beads can then be used for a variety of downstream applications including IP, Co-IP, chromatin IP (ChIP), RNA IP (RIP), small-scale IgG purification, and protein purification. Bound material is easily collected using a DynaMag magnet due to the unique magnetic properties of the Dynabeads. The recombinant protein G on the beads contains no albumin binding sites, thus albumin is not co-purified during the procedure. The IP is fast and gives high yield, high reproducibility, and very little non-specific binding, thus pre-clearing is not required.

Automated Dynabeads separation helps increase throughput and reduces hands-on time
If you are working with several samples in parallel, the number of washing steps and the hands-on time increases proportionally with the number of samples. Pipetting and other manual handling tend to be less consistent than automation when working with many samples at a time. To better handle a medium- to high-throughput number of samples, reduce hands-on time, and secure high reproducibility, we have developed IP protocols for the KingFisher Flex and KingFisher Duo Prime instruments. The automated protocols replicate the manual protocols, obtaining equally high target protein yield and the same low non-specific binding and high reproducibility. It doesn’t matter if you are working with 10 or 96 samples, the IP protocol is less than 40 minutes regardless. Just load the reagents on the plates, push the “Start” button and by the time you have prepared for downstream analysis, the IP is done. Some optimization (e.g., incubation times) might be necessary depending on your antibody and the abundance and/or specificity of your target protein.

• Use the KingFisher Duo instrument for low to medium throughput (1-12 samples/run)
• Use the KingFisher Flex instrument for high throughput (12-96 samples/run)
See automated protocols
Watch a video about the KingFisher Flex instrument

Gentle separation causes minimal physical stress to proteins
The magnetic separation technology utilized by Dynabeads Protein G is rapid and gentle, causing minimal physical stress to your target proteins. This permits the isolation and concentration of labile composites that might otherwise dissociate or be damaged by proteases during long incubation times. Native protein conformation and large protein complexes are preserved.

Binding strength and capacity
Dynabeads Protein G allow for isolation of most mammalian immunoglobulins (Ig). The amount of Ig captured depends on the concentration of Ig in the starting sample and on the type and source of the Ig. 100 µL of Dynabeads Protein G will isolate approximately 25–30 µg human IgG from a sample containing 20–200 µg IgG/mL. Predominant Fc-binding allows optimal Ig orientation. The antibodies bind to the outer smooth surface of the beads, thus are not trapped in large pores as with Sepharose/agarose-based beads. All antibodies are available for protein binding, so low amounts of antibody are required while still obtaining the same high yield of target protein. The smooth bead surface is also responsible for the low non-specific binding that Dynabeads are known for.

Learn more about Dynabeads
• Dynabeads Protein G are also available as a stand-alone reagent without included buffers
See immunoprecipitation selection guides, data, and references
See magnets for Dynabeads separations
Find Dynabeads products for other applications

OEM purchase
To purchase Dynabeads Protein A and Protein G on an OEM basis, contact our Out-Licensing and OEM Sales department.

*Sepharose is a trademark of GE Healthcare Bio-Sciences AB.

MagnaBind™ Protein G Beads (Thermo Scientific™)

Thermo Scientific™ MagnaBind™ Beads provide a convenient method for magnetic separation of antibodies, antigens, lectins, enzymes, nucleic acids and cells using affinity binding. To remove the MagnaBind Beads from the suspension, an external magnetic field is used.

Features of MagnaBind Protein G Beads:

Composition: Silanized iron oxide
Magnetization: 25-35EMU/g
Type of Magnetization: Superparamagnetic (no magnetic memory)
Surface Area: >100m2/g
Bead Size: 1-4µm diameter
Settling Rate: 4% in 30 minutes
Effective Density: 2.5g/mL
Number of Beads: 1 × 108 beads/mg
pH Stability: Aqueous solution, above pH 4.0
Concentration: 5 mg/mL

MagnaBind Protein G Beads are typically used to isolate antibodies from serum and cell culture supernatant and to separate cells of interest from a cell mixture. For antibody purification, the beads are incubated with the antibody solution and then magnetically separated from the supernatant. The antigens and antibodies are then dissociated from the beads using an elution buffer. For cell separation, the monoclonal or polyclonal antibody to the cell surface antigen is incubated with Protein G magnetic beads, magnetically separated, and then incubated with the cell suspension.

Applications:
• Cell sorting using either positive or negative selection
• Protein purification or immunoassays using either direct or indirect methods

Dynabeads™ Protein A Immunoprecipitation Kit (Invitrogen™)

The Dynabeads™ Protein A Immunoprecipitation Kit is a faster and easier solution for immunoprecipitation (IP) than using Sepharose™ resin or agarose resin, and includes all reagents and buffers required to perform IP using your own antibody. The kit is optimized for standard IP, but can also be used for Co-IP, chromatin IP (ChIP), or RNA IP (RIP). The kit includes the widely used Dynabeads Protein A with recombinant Protein A (~45 kDa) covalently coupled to the magnetic bead surface. Both manual and automated protocols are available.

• IP in less than 40 minutes
• High target protein yield with low antibody consumption
• Very low non-specific binding with high signal-to-noise ratio
• No columns, centrifugations, or time-consuming pre-clearing required
• High reproducibility with Dynabeads Protein A and all buffers supplied in the kit
• High throughput compatible with KingFisher™ instruments

Manual Dynabeads separation is fast and easy to perform
The manual protocol is simple and can be performed in under 40 minutes. First, the antibody for the target protein is incubated with the Dynabeads Protein A in a tube for 10 minutes. Excess antibody is washed away by placing the tube in a DynaMag™ magnet and removing the supernatant. The antibody-coated beads can then be used for a variety of downstream applications including IP, Co-IP, chromatin IP (ChIP), RNA IP (RIP), small-scale IgG purification, and protein purification. Bound material is easily collected using a DynaMag magnet due to the unique magnetic properties of the Dynabeads. The recombinant protein A on the beads contains no albumin binding sites, thus albumin is not co-purified during the procedure. The IP is fast and gives high yield, high reproducibility, and very little non-specific binding, thus pre-clearing is not required.

Automated Dynabeads separation helps increase throughput and reduces hands-on time
If you are working with several samples in parallel, the number of washing steps and the hands-on time increases proportionally with the number of samples. Pipetting and other manual handling tend to be less consistent than automation when working with many samples at a time. To better handle a medium- to high-throughput number of samples, reduce hands-on time, and secure high reproducibility, we have developed IP protocols for the KingFisher Flex and KingFisher Duo Prime instruments. The automated protocols replicate the manual protocols, obtaining equally high target protein yield and the same low non-specific binding and high reproducibility. It doesn’t matter if you are working with 10 or 96 samples, the IP protocol is less than 40 minutes regardless. Just load the reagents on the plates, push the “Start” button and by the time you have prepared for downstream analysis, the IP is done. Some optimization (e.g., incubation times) might be necessary depending on your antibody and the abundance and/or specificity of your target protein.

• Use the KingFisher Duo instrument for low to medium throughput (1-12 samples/run)
• Use the KingFisher Flex instrument for high throughput (12-96 samples/run)
See automated protocols
Watch a video about the KingFisher Flex instrument

Gentle separation causes minimal physical stress to proteins
The magnetic separation technology utilized by Dynabeads Protein A is rapid and gentle, causing minimal physical stress to your target proteins. This permits the isolation and concentration of labile composites that might otherwise dissociate or be damaged by proteases during long incubation times. Native protein conformation and large protein complexes are preserved.

Binding strength and capacity
Dynabeads Protein A allow for isolation of most mammalian immunoglobulins (Ig). The amount of Ig captured depends on the concentration of Ig in the starting sample and on the type and source of the Ig. 100 µL of Dynabeads Protein A will isolate approximately 25–30 µg human IgG from a sample containing 20–200 µg IgG/mL. Predominant Fc-binding allows optimal Ig orientation. The antibodies bind to the outer smooth surface of the beads, thus are not trapped in large pores as with Sepharose/agarose-based beads. All antibodies are available for protein binding, so low amounts of antibody are required while still obtaining the same high yield of target protein. The smooth bead surface is also responsible for the low non-specific binding that Dynabeads are known for.

Learn more about Dynabeads
• Dynabeads Protein A are also available as a stand-alone reagent without included buffers
See immunoprecipitation selection guides, data, and references
See magnets for Dynabeads separations
Find Dynabeads products for other applications

OEM purchase
To purchase Dynabeads Protein A and Protein G on an OEM basis, contact our Out-Licensing and OEM Sales department.

*Sepharose is a trademark of GE Healthcare Bio-Sciences AB.