Shop All DNA Ladders

50 bp DNA Ladder (Invitrogen™)

Invitrogen 50 bp DNA Ladder is designed for sizing and approximate quantification of double-stranded DNA in the range of 50 bp to 2,500 bp. 50 bp DNA Ladder consists of 17 individual chromatography-purified DNA fragments and has reference bands at 2500, 800, and 350 bp for easy orientation.

50 bp DNA Ladder is ideal for separation on 2% agarose gels.

Highlights of 50 bp DNA Ladder:
Sharp, clear bands—chromatography purified fragments for consistent and reliable results
Convenient—provided with 6X TrackIt Cyan/Orange Loading Buffer for tracking of DNA migration
Precise—an exact amount of DNA in each band

Product use
The double-stranded DNA ladder can be visualized on 2% agarose gels after ethidium bromide or SYBR Safe staining. The ladder is designed with a uniform intensity of DNA bands for a clear view of each band. An exact amount of DNA in each band allows approximate quantification of DNA samples.

This ladder can be radiolabeled with T4 polynucleotide kinase or T4 DNA polymerase.

GeneRuler 1 kb DNA Ladder (Thermo Scientific™)

Thermo Scientific GeneRuler 1 kb DNA Ladder is recommended for sizing and approximate quantification of a double-stranded DNA in the range of 250 bp to 10,000 bp on agarose gels. The DNA ladder consists of 14 DNA fragments and is provided with 6X TriTrack DNA Loading Dye.

Highlights of 1 kb DNA Ladder
Easy visualization—three-color tracking of DNA migration during electrophoresis with 6X TriTrack DNA Loading Dye (contains Xylene Cyanol FF, Bromophenol Blue, and Orange G)
Simplicity—one loading dye for a variety of samples
Sharp bands—clear results with mixtures of individual chromatography-purified DNA fragments

Product use
This double-stranded DNA ladder is compatible with 1% standard and precast agarose slab gels and can be visualized after ethidium bromide or SYBR Safe staining. A uniform intensity of bands and bright reference bands allow easy sizing and orientation. A defined amount of DNA in each band enables approximate quantification of sample DNA.

NoLimits 15 bp DNA Fragment (Thermo Scientific™)

Thermo Scientific NoLimits is a collection of 36 highly-purified individual DNA fragments ranging from 10 to 20,000 bp. Unlike other vendors, where custom standards are produced by PCR, Thermo Scientific NoLimits DNA fragments are produced using specifically designed plasmids purified by patented technology.Plasmid DNA, digested with Thermo Scientific Restriction Enzymes, is chromatography-purified to prepare the individual DNA fragments from the digestion mixture. The results are extremely pure DNA fragments, free of any truncated or degraded molecules. These custom DNA fragments are the best custom standards for applications ranging from electrophoresis (both gel and capillary) to HPLC and beyond.

Features

• Broad range: 10-20,000 bp
• Sharp peaks during capillary electrophoresis, sharp bands during gel electrophoresis
• Precise DNA concentration
• Chromatography-purified individual DNA fragments

Applications

• Gel electrophoresis
• Capillary electrophoresis
• DNA quantification
• HPLC analysis
• Applications where a custom DNA size standard is required.

GeneRuler 100 bp DNA Ladder, ready-to-use (Thermo Scientific™)

Thermo Scientific GeneRuler 100 bp DNA Ladder is recommended for sizing and approximate quantification of a double-stranded DNA in the range of 100 bp to 1,000 bp on agarose or polyacrylamide gels. The DNA ladder consists of 10 DNA fragments and is provided with 6X TriTrack DNA Loading Dye.

Highlights of 100 bp DNA Ladder
Easy visualization—three-color tracking of DNA migration during electrophoresis with 6X TriTrack DNA Loading Dye (contains Xylene Cyanol FF, Bromophenol Blue, and Orange G)
Simplicity—one loading dye for a variety of samples
Sharp bands—clear results with mixtures of individual chromatography-purified DNA fragments
Convenient—ready-to-load DNA Ladder is premixed with 6X TriTrack DNA Loading dye and stable at room temperature for up to 6 months

Product use
This double-stranded DNA ladder is compatible with 1–2 % standard and precast agarose slab gels and can be visualized after ethidium bromide or SYBR Safe staining. A uniform intensity of bands and bright reference bands allow easy sizing and orientation. A defined amount of DNA in each band enables approximate quantification of sample DNA.

NoLimits 750 bp DNA Fragment (Thermo Scientific™)

Thermo Scientific NoLimits is a collection of 36 highly-purified individual DNA fragments ranging from 10 to 20,000 bp. Unlike other vendors, where custom standards are produced by PCR, Thermo Scientific NoLimits DNA fragments are produced using specifically designed plasmids purified by patented technology.Plasmid DNA, digested with Thermo Scientific Restriction Enzymes, is chromatography-purified to prepare the individual DNA fragments from the digestion mixture. The results are extremely pure DNA fragments, free of any truncated or degraded molecules. These custom DNA fragments are the best custom standards for applications ranging from electrophoresis (both gel and capillary) to HPLC and beyond.

Features

• Broad range: 10-20,000 bp
• Sharp peaks during capillary electrophoresis, sharp bands during gel electrophoresis
• Precise DNA concentration
• Chromatography-purified individual DNA fragments

Applications

• Gel electrophoresis
• Capillary electrophoresis
• DNA quantification
• HPLC analysis
• Applications where a custom DNA size standard is required.

NoLimits 10 bp DNA Fragment (Thermo Scientific™)

Thermo Scientific NoLimits is a collection of 36 highly-purified individual DNA fragments ranging from 10 to 20,000 bp. Unlike other vendors, where custom standards are produced by PCR, Thermo Scientific NoLimits DNA fragments are produced using specifically designed plasmids purified by patented technology.

Plasmid DNA is digested with Thermo Scientific Restriction Enzymes, and the individual DNA fragments are chromatography-purified from the digestion mixture. As a result, we offer exceptionally pure DNA fragments that are free of any truncated or degraded molecules. Our custom DNA fragments are the best choice for applications, ranging from electrophoresis (both gel and capillary) to HPLC and beyond.

Highlights

• Broad range: 10-20,000 bp
• Sharp peaks during capillary electrophoresis
• Sharp bands during gel electrophoresis
• Precise DNA concentration
• Chromatography-purified individual DNA fragments

Applications

• Gel electrophoresis
• Capillary electrophoresis
• DNA quantification
• HPLC analysis
• Applications where a custom DNA size standard is required.

MassRuler Express Forward DNA Ladder Mix, ready-to-use (Thermo Scientific™)

Thermo Scientific MassRuler Express Forward and Reverse DNA Ladder mixes are specially designed for fast and reliable DNA quantification in short separation distances under various electrophoresis conditions (see Table 1). In the forward versions, the mass of each DNA fragment is directly proportional to the fragment size whereas, in the reverse versions, the mass is inversely proportional to fragment's size.

The intensity of the fragment in each ladder is calibrated against a standard that guarantees the precise quantity of each band. The ladders are mixtures of chromatography-purified, individual DNA fragments.

Highlights
• Accurate DNA sizing and quantification—DNA ladder fragments are quantified spectrophotometrically
• Fast and reliable DNA quantification &mdash short separation distances under various electrophoresis conditions
• Sharp bands
• Easy-to-remember fragment sizes and quantities
• Ready-to-use—premixed with 6X MassRuler DNA Loading Dye for direct loading and room temperature storage
• Supplied with loading dye for sample DNA

GeneRuler 1 kb DNA Ladder, ready-to-use (Thermo Scientific™)

Thermo Scientific GeneRuler 1 kb DNA Ladder is recommended for sizing and approximate quantification of a double-stranded DNA in the range of 250 bp to 10,000 bp on agarose gels. The DNA ladder consists of 14 DNA fragments and is provided with 6X TriTrack DNA Loading Dye.

Highlights of 1 kb DNA Ladder
Easy visualization—three-color tracking of DNA migration during electrophoresis with 6X TriTrack DNA Loading Dye (contains Xylene Cyanol FF, Bromophenol Blue, and Orange G)
Simplicity—one loading dye for a variety of samples
Sharp bands—clear results with mixtures of individual chromatography-purified DNA fragments
Convenient—ready-to-load DNA Ladder is premixed with 6X TriTrack DNA Loading dye and stable at room temperature for up to 6 months

Product use
This double-stranded DNA ladder is compatible with 1% standard and precast agarose slab gels and can be visualized after ethidium bromide or SYBR Safe staining. A uniform intensity of bands and bright reference bands allow easy sizing and orientation. A defined amount of DNA in each band enables approximate quantification of sample DNA.

NoLimits 800 bp DNA Fragment (Thermo Scientific™)

Thermo Scientific NoLimits is a collection of 36 highly-purified individual DNA fragments ranging from 10 to 20,000 bp. Unlike other vendors, where custom standards are produced by PCR, Thermo Scientific NoLimits DNA fragments are produced using specifically designed plasmids purified by patented technology.Plasmid DNA, digested with Thermo Scientific Restriction Enzymes, is chromatography-purified to prepare the individual DNA fragments from the digestion mixture. The results are extremely pure DNA fragments, free of any truncated or degraded molecules. These custom DNA fragments are the best custom standards for applications ranging from electrophoresis (both gel and capillary) to HPLC and beyond.

Features

• Broad range: 10-20,000 bp
• Sharp peaks during capillary electrophoresis, sharp bands during gel electrophoresis
• Precise DNA concentration
• Chromatography-purified individual DNA fragments

Applications

• Gel electrophoresis
• Capillary electrophoresis
• DNA quantification
• HPLC analysis
• Applications where a custom DNA size standard is required.

NoLimits 20 bp DNA Fragment (Thermo Scientific™)

Thermo Scientific NoLimits is a collection of 36 highly-purified individual DNA fragments ranging from 10 to 20,000 bp. Unlike other vendors, where custom standards are produced by PCR, Thermo Scientific NoLimits DNA fragments are produced using specifically designed plasmids purified by patented technology.Plasmid DNA, digested with Thermo Scientific Restriction Enzymes, is chromatography-purified to prepare the individual DNA fragments from the digestion mixture. The results are extremely pure DNA fragments, free of any truncated or degraded molecules. These custom DNA fragments are the best custom standards for applications ranging from electrophoresis (both gel and capillary) to HPLC and beyond.

Features

• Broad range: 10-20,000 bp
• Sharp peaks during capillary electrophoresis, sharp bands during gel electrophoresis
• Precise DNA concentration
• Chromatography-purified individual DNA fragments

Applications

• Gel electrophoresis
• Capillary electrophoresis
• DNA quantification
• HPLC analysis
• Applications where a custom DNA size standard is required.

1 Kb Plus DNA Ladder (Invitrogen™)

Invitrogen 1 Kb Plus DNA Ladder is designed for sizing and approximate quantification of double-stranded DNA in the range of 100 bp to 15,000 bp. 1 Kb Plus DNA Ladder consists of 18 individual chromatography-purified DNA fragments and has a reference band at 1,500 bp for easy orientation.

1 Kb DNA Ladder is ideal for separation on 0.8–1% agarose gels.

Highlights of 1 Kb Plus DNA Ladder:
Sharp, clear bands—chromatography purified fragments for consistent and reliable results
Convenient—provided with 10X BlueJuice Gel Loading Buffer for tracking of sample DNA migration
Precise—an exact amount of DNA in each band

Product use
The double-stranded DNA ladder can be visualized on 0.8–1% agarose gels after ethidium bromide or SYBR Safe staining. The ladder is designed with a uniform intensity of DNA bands for a clear view of each band. An exact amount of DNA in each band allows approximate quantification of DNA samples.

This ladder can be radiolabeled with T4 polynucleotide kinase or T4 DNA polymerase.

E-Gel™ Sizing DNA Ladder (Invitrogen™)

Invitrogen E-Gel Sizing DNA Ladder is designed for sizing and approximate quantification of double-stranded DNA in the range of 50 to 1,500 bp. E-Gel Sizing DNA Ladder consists of 19 individual chromatography-purified DNA fragments with reference bands at 1000, 500, and 200 bp for easy orientation.

E-Gel Sizing DNA Ladder is specifically formulated for optimal performance on pre-cast E-Gel agarose gels.

Highlights of E-Gel Sizing DNA Ladder:
Performance—optimized for use on E-Gel SizeSelect ll agarose gels
Sharp, clear bands—chromatography-purified fragments for consistent and reliable results, stable at room temperature for up to six months
Ready to use—premixed with loading buffer
Convenient—provided with 1X E-Gel Sample Loading Buffer
Precise—an exact amount of DNA in each band

Product use
The double-stranded DNA ladder can be visualized on 2% E-Gel SizeSelect II and E-Gel EX agarose gels. The ladder is designed with a uniform intensity of DNA bands for a clear view of each band. An exact amount of DNA in each band allows approximate quantification of DNA samples.

O’RangeRuler 5 bp DNA Ladder, ready-to-use (Thermo Scientific™)

Thermo Scientific O'RangeRuler 5 bp DNA Ladder is designed for precise sizing of PCR products and other double-stranded DNA fragments in agarose or non-denaturing polyacrylamide gels. It is a step ladder with 5 bp differences between the DNA fragments. It consists of purified and ligated blunt-ended basic unit repeats of 5 bp. Not recommended for DNA quantification.

O'RangeRuler 5 bp DNA Ladder is supplied with 6X Orange DNA Loading Dye for sample DNA. Orange G dye can be used to monitor DNA migration in agarose or polyacrylamide gels and migrates at 50 bp in a 1% agarose gel. It is the preferable dye when visualization of small DNA fragments is important.

Highlights

• Step ladder with 5 bp increments
• Sharp bands, easy-to-remember fragment sizes
• Evenly spaced reference bands
• Ready-to-use—premixed with 6X Orange Loading Dye for direct loading and room temperature storage
• Supplied with loading dye for sample DNA

Lambda DNA/HindIII Marker (Thermo Scientific™)

Thermo Scientific Lambda DNA/HindIII Marker is recommended for sizing of linear double-stranded large DNA fragments in agarose gels. Lambda DNA is digested to completion with the appropriate Thermo Scientific restriction enzyme(s) and purified and dissolved in storage buffer. DNA fragments contain various sticky ends depending on the restriction enzyme used for preparation of the marker.

The marker can be labeled radioactively with T4 Polynucleotide Kinase. Alternatively, and it can be labeled radioactively or non-radioactively with Klenow Fragment or Klenow Fragment, exo- with the fill-in reaction (see Resources for Protocols).

Highlights

• Sizing and approximate quantification of large DNA fragments
• Sharp bands
• Supplied with loading dye for sample DNA

Note
The cohesive ends (the 12 nt cos site of lambda DNA) of fragments indicated may anneal and form additional bands. These fragments can be separated by heating at 65°C for 5 minutes and then cooling on ice for 3 minutes.

phiX174 DNA/BsuRI (HaeIII) Marker (Thermo Scientific™)

Thermo Scientific phiX174 DNA is digested to completion with the appropriate Thermo Scientific restriction enzyme, then purified and dissolved in storage buffer. The DNA fragments contain blunt or sticky ends, depending on the restriction enzyme used for the markers preparation.

Thermo Scientific phiX174 DNA/BsuRI (HaeIII) Marker can be labeled radioactively with T4 Polynucleotide Kinase. Alternatively it can be labeled radioactively or non-radioactively with Klenow fragment or Klenow Fragment, exo- with the fill-in reaction, see protocols in Resources.

Highlights

• Sizing and approximate quantification of small DNA fragments
• Sharp bands
• Supplied with loading dye for sample DNA

Note
• Bands at 310, 281, and 271 bp form an anomalous pattern on polyacrylamide gels.
• The shortest fragments (oblique) are not visible in standard electrophoresis.
• Fragment lengths are predicted by computer analysis of the respective DNA sequences.