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BenchMark™ Pre-stained Protein Ladder (Invitrogen™)

BenchMark Pre-Stained Protein Ladder contains 10 proteins ranging in apparent molecular weight from 6 to 180 kDa for use in monitoring electrophoresis or transfer from gel to membrane in western blotting. The protein standard is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

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Applications
• Monitoring protein migration during SDS-polyacrylamide gel electrophoresis
• Monitoring protein transfer onto membranes after western blotting
• Sizing of proteins on SDS-PAGE gels and western blots

Protein Molecular Weight Standards (broad range) (Invitrogen™)

The broad range protein molecular standards include 11 proteins from 6500 to 205,000 daltons for use as molecular weight standards in SDS-polyacrylamide gel electrophoresis.

Spectra™ Multicolor High Range Protein Ladder (Thermo Scientific™)

Thermo Scientific Spectra Multicolor High Range Protein Ladder is a mixture of eight blue-, green-, and orange-stained proteins (40 to 300 kDa) for use as size standards for high-MW proteins in gel electrophoresis and western blotting. The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

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Applications
• Monitoring of protein migration during SDS-PAGE
• Verifying western transfer efficiency
• Approximate sizing of proteins on SDS-polyacrylamide gels and western blots
• Locating a protein of interest for excision from an unstained preparative gel

E-PAGE™ SeeBlue™ Pre-stained Standard (Invitrogen™)

E-PAGE SeeBlue Pre-stained Standards are designed for use with the pre-cast E-PAGE Electrophoresis System. The standards consist of three blue-dyed and two colored bands ranging in size from ~21–261 kDa for monitoring gel and transfer runs. The protein standard is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

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Applications
• Monitoring protein migration during SDS-polyacrylamide gel electrophoresis
• Monitoring protein transfer onto membranes after western blotting
• Sizing of proteins on E-PAGE gels and western blots

PageRuler™ Plus Prestained Protein Ladder, 10 to 250 kDa (Thermo Scientific™)

Thermo Scientific PageRuler Plus Prestained Protein Ladder is a mixture of nine blue-, orange-, and green-stained proteins (10 to 250 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and western blotting. The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

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Applications
• Monitoring protein migration during SDS-polyacrylamide gel electrophoresis
• Monitoring protein transfer onto membranes after western blotting
• Sizing of proteins on SDS-polyacrylamide gels and western blots

BenchMark™ Protein Ladder (Invitrogen™)

BenchMark Protein Ladder consists of 15 unstained recombinant proteins ranging in molecular weight from 10 to 220 kDa. The protein standard is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

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Applications
• Sizing of proteins on SDS-polyacrylamide gels and western blots

Pierce™ Prestained Protein MW Marker (Thermo Scientific™)

Thermo Scientific Prestained Protein Molecular Weight Marker is a mixture of six blue-stained natural proteins (20 to 120 kDa) for use as size standards in protein electrophoresis (SDS-PAGE). The protein standards are supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

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Component proteins: beta-galactosidase (120 kDa), bovine serum albumin (85 kDa), ovalbumin (50 kDa), cardonic anhydrase (35 kDa), beta-lactoglobulin (25 kDa), and lysozyme (20 kDa)

Applications
• Monitoring protein migration during SDS-polyacrylamide gel electrophoresis.
• Monitoring protein transfer onto membranes after western blotting
• Sizing of proteins on SDS-polyacrylamide gels and western blots

PageRuler™ Unstained High Range Protein Ladder (Thermo Scientific™)

Thermo Scientific PageRuler Unstained High Range Protein Ladder is a mixture of eight (8) unstained proteins (60 to 250kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.

Features of PageRuler Unstained High Range Protein Ladder:

Size range—8 proteins spanning 60 to 250kDa
Ready-to-use—supplied in a loading buffer for direct loading on gels
Sharp bands—excellent accuracy for detection in with coomassie or silver stains
Reference band—150kDa band has greater intensity for easy orientation
Tagged—each protein in the ladder contains an integral Strep-tag™ II Sequence
Quality tested—each lot evaluated by SDS-PAGE and Western blotting

The protein MW markers in this ladder resolve into clearly identifiable sharp bands when analyzed by SDS-PAGE and stained with coomassie dye or silver. The 150kDa band is of greater intensity and serves as a reference band. Proteins can be detected on Western blots by staining with Ponceau S, coomassie blue or other protein stains. In addition, the ladder proteins contain an integral Step-tag™ II Sequence and may be detected on Western blots using Strep-Tactin™ Conjugates. The PageRuler Unstained High Range Protein Ladder is supplied ready-to-use: no heating, dilution or addition of a reducing agent is required before use.

Includes:
• Proteins (0.02 to 0.05 mg/mL of each) in 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 100 mM DTT, 1 mM NaN3, 0.01% bromophenol blue and 33% glycerol

Applications:
• Accurate protein sizing on SDS-polyacrylamide gels and Western blots.

Pierce™ Unstained Protein MW Marker (Thermo Scientific™)

Thermo Scientific Unstained Protein Molecular Weight Marker is a mixture of seven native proteins (14.4 to 116 kDa) for use as size standards in protein electrophoresis (SDS-PAGE).

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Applications
• Accurate protein sizing on SDS-polyacrylamide gels and western blots

PeppermintStick™ Phosphoprotein Molecular Weight Standards (Invitrogen™)

PeppermintStick Phosphoprotein Molecular Weight Standards are a mixture of phosphorylated and nonphosphorylated proteins ranging from 14.4 to 116 kDa. Separation by polyacrylamide gel electrophoresis resolves the standard mixture into two phosphorylated and four nonphosphorylated protein bands.

Applications
• Accurate protein sizing on SDS-polyacrylamide gels and western blots.
• Positive and negative controls for detection of phosphorylated proteins, such as the methods provided by our Pro-Q® Diamond phosphoprotein gel stain

SeeBlue™ Pre-stained Protein Standard (Invitrogen™)

SeeBlue Pre-Stained Standard consists of nine polypeptides that resolve into sharp, tight blue bands in the range of 4–250 kDa allowing you to easily monitor protein electrophoresis and western transfer efficiency. The protein standard is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

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Applications
• Monitoring protein migration during SDS-polyacrylamide gel electrophoresis.
• Monitoring protein transfer onto membranes after western blotting
• Sizing of proteins on SDS-polyacrylamide gels and western blots

MagicMark™ XP Western Protein Standard (Invitrogen™)

MagicMark XP Western Protein Standard consists of nine recombinant proteins (20–220 kDa), each of which contains an IgG binding site. The IgG binding site binds the primary or secondary antibody used for detection of the target protein, allowing direct visualization of the standard on the western blot. The MagicMark XP Standard is compatible with most western kits and substrates (chemiluminescent, chromogenic, and fluorescent). The protein standard is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

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Applications
• Western blotting: detection of the nine unstained bands via the detection method used for the target protein. Compatible with chemiluminescent substrates and fluorescent secondary antibodies (not recommended for antibodies labeled with fluors in the 500–550 nm channel).
• Protein size estimation: protein sizing on SDS-polyacrylamide gels and on western blot using western blot detection method