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PageRuler™ Plus Prestained Protein Ladder, 10 to 250 kDa (Thermo Scientific™)

Thermo Scientific PageRuler Plus Prestained Protein Ladder is a mixture of nine blue-, orange-, and green-stained proteins (10 to 250 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and western blotting. The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

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Applications
• Monitoring protein migration during SDS-polyacrylamide gel electrophoresis
• Monitoring protein transfer onto membranes after western blotting
• Sizing of proteins on SDS-polyacrylamide gels and western blots

PeppermintStick™ Phosphoprotein Molecular Weight Standards (Invitrogen™)

PeppermintStick Phosphoprotein Molecular Weight Standards are a mixture of phosphorylated and nonphosphorylated proteins ranging from 14.4 to 116 kDa. Separation by polyacrylamide gel electrophoresis resolves the standard mixture into two phosphorylated and four nonphosphorylated protein bands.

Applications
• Accurate protein sizing on SDS-polyacrylamide gels and western blots.
• Positive and negative controls for detection of phosphorylated proteins, such as the methods provided by our Pro-Q® Diamond phosphoprotein gel stain

IEF Marker 3-10 (Invitrogen™)

IEF Marker 3-10 is a ready-to-use protein marker consisting of 13 purified isoforms for the use in IEF applications. The marker works well on all horizontal and vertical one-dimensional IEF gels and can be used in both native and denaturing conditions.

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Applications
• Monitoring of separation on IEF gels

PageRuler™ Unstained Protein Ladder (Thermo Scientific™)

Thermo Scientific PageRuler Unstained Protein Ladder is a mixture of 14 recombinant, highly purified, unstained proteins (10 to 200 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and western blotting. The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

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Product features
• Reference bands—50 kDa band is stronger than the others for easy orientation
• Tagged—the ladder proteins contains an integral Strep-tag™ II sequence and can be detected on western blots using Strep-Tactin™ conjugates or an antibody against Strep-tag™ II Sequence

Applications
• Accurate protein sizing on SDS-polyacrylamide gels and western blots

SeeBlue™ Pre-stained Protein Standard (Invitrogen™)

SeeBlue Pre-Stained Standard consists of nine polypeptides that resolve into sharp, tight blue bands in the range of 4–250 kDa allowing you to easily monitor protein electrophoresis and western transfer efficiency. The protein standard is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

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Applications
• Monitoring protein migration during SDS-polyacrylamide gel electrophoresis.
• Monitoring protein transfer onto membranes after western blotting
• Sizing of proteins on SDS-polyacrylamide gels and western blots

PageRuler™ Unstained Broad Range Protein Ladder (Thermo Scientific™)

Thermo Scientific PageRuler Unstained Broad Range Protein Ladder is a mixture of 11 proteins (5 to 250 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and western blotting. The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

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Product features
• Reference bands—the 100, 50, and 20 kDa bands are more intense for easy orientation
• Tagged— each protein in the contains an integral Strep-tag™ II sequence and can be detected on western blots using Strep-Tactin™ conjugates or an antibody against Strep-tag™ II sequence

Applications
• Accurate protein sizing on SDS-polyacrylamide gels and western blots

Novex™ Sharp Unstained Protein Standard (Invitrogen™)

Novex Sharp Unstained Protein Standard is designed for accurate and convenient molecular weight estimation of proteins during SDS-PAGE and western blotting. The standard consists of a wide range of molecular weight bands consisting of 12 proteins in the range of 3.5–260 kDa. The protein standard is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

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Applications
• Accurate protein sizing on SDS-polyacrylamide gels and western blots

BenchMark™ His-tagged Protein Standard (Invitrogen™)

BenchMark His-tagged Protein Standard is designed as a positive control and for molecular weight sizing in his-tagged fusion protein detection. Each of the ten standard proteins (10-160 kDa) contains a 6X His-tag, enabling the detection of standard proteins with the InVision™ His-tag In-gel Stain (Cat. no. LC6030) or with Anti-His(C-term) Antibody (Cat. no. R930-25) using western detection. The protein standard is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

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Applications
• Verification of His-tagged fusion protein detection using InVision In-Gel Stain or an Anti-His(C-term) antibody
• Sizing of proteins on SDS-polyacrylamide gels and western blots

Spectra™ Multicolor Low Range Protein Ladder (Thermo Scientific™)

Thermo Scientific Spectra Multicolor Low Range Protein Ladder is a mixture of six proteins and synthetic peptides (1.7 to 40 kDa) for use as size standards for peptides and small proteins in gel electrophoresis and western blotting. The protein standard is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

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Applications
• Monitoring of protein migration during SDS-PAGE
• Verifying western transfer efficiency
• Approximate sizing of proteins on SDS-polyacrylamide gels and western blots
• Locating a protein of interest for excision from an unstained preparative gel

E-PAGE™ SeeBlue™ Pre-stained Standard (Invitrogen™)

E-PAGE SeeBlue Pre-stained Standards are designed for use with the pre-cast E-PAGE Electrophoresis System. The standards consist of three blue-dyed and two colored bands ranging in size from ~21–261 kDa for monitoring gel and transfer runs. The protein standard is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

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Applications
• Monitoring protein migration during SDS-polyacrylamide gel electrophoresis
• Monitoring protein transfer onto membranes after western blotting
• Sizing of proteins on E-PAGE gels and western blots

MagicMark™ XP Western Protein Standard (Invitrogen™)

MagicMark XP Western Protein Standard consists of nine recombinant proteins (20–220 kDa), each of which contains an IgG binding site. The IgG binding site binds the primary or secondary antibody used for detection of the target protein, allowing direct visualization of the standard on the western blot. The MagicMark XP Standard is compatible with most western kits and substrates (chemiluminescent, chromogenic, and fluorescent). The protein standard is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

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Applications
• Western blotting: detection of the nine unstained bands via the detection method used for the target protein. Compatible with chemiluminescent substrates and fluorescent secondary antibodies (not recommended for antibodies labeled with fluors in the 500–550 nm channel).
• Protein size estimation: protein sizing on SDS-polyacrylamide gels and on western blot using western blot detection method

Spectra™ Multicolor Broad Range Protein Ladder (Thermo Scientific™)

Thermo Scientific Spectra Multicolor Broad Range Protein Ladder is a 4-color (blue, orange, green, pink) protein standard containing 10 prestained proteins (10 to 260 kDa) for use in gel electrophoresis and western blotting. The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

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Applications
• Monitoring protein migration during SDS-polyacrylamide gel electrophoresis
• Monitoring protein transfer onto membranes after western blotting
• Sizing of proteins on SDS-polyacrylamide gels and western blots