Shop All Protein Standards & Ladders

Spectra™ Multicolor Low Range Protein Ladder (Thermo Scientific™)

Thermo Scientific Spectra Multicolor Low Range Protein Ladder is a mixture of six proteins and synthetic peptides (1.7 to 40 kDa) for use as size standards for peptides and small proteins in gel electrophoresis and western blotting. The protein standard is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

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Applications
• Monitoring of protein migration during SDS-PAGE
• Verifying western transfer efficiency
• Approximate sizing of proteins on SDS-polyacrylamide gels and western blots
• Locating a protein of interest for excision from an unstained preparative gel

PageRuler™ Unstained Protein Ladder (Thermo Scientific™)

Thermo Scientific PageRuler Unstained Protein Ladder is a mixture of 14 recombinant, highly purified, unstained proteins (10 to 200 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and western blotting. The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

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Product features
• Reference bands—50 kDa band is stronger than the others for easy orientation
• Tagged—the ladder proteins contains an integral Strep-tag™ II sequence and can be detected on western blots using Strep-Tactin™ conjugates or an antibody against Strep-tag™ II Sequence

Applications
• Accurate protein sizing on SDS-polyacrylamide gels and western blots

Novex™ Sharp Unstained Protein Standard (Invitrogen™)

Novex Sharp Unstained Protein Standard is designed for accurate and convenient molecular weight estimation of proteins during SDS-PAGE and western blotting. The standard consists of a wide range of molecular weight bands consisting of 12 proteins in the range of 3.5–260 kDa. The protein standard is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

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Applications
• Accurate protein sizing on SDS-polyacrylamide gels and western blots

CandyCane™ Glycoprotein Molecular Weight Standards (Invitrogen™)

CandyCane glycoprotein molecular weight standards separate into alternating bands of glycosylated and nonglycosylated proteins during polyacrylamide gel electrophoresis.

IEF Marker 3-10 (Invitrogen™)

IEF Marker 3-10 is a ready-to-use protein marker consisting of 13 purified isoforms for the use in IEF applications. The marker works well on all horizontal and vertical one-dimensional IEF gels and can be used in both native and denaturing conditions.

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Applications
• Monitoring of separation on IEF gels

Spectra™ Multicolor Broad Range Protein Ladder (Thermo Scientific™)

Thermo Scientific Spectra Multicolor Broad Range Protein Ladder is a 4-color (blue, orange, green, pink) protein standard containing 10 prestained proteins (10 to 260 kDa) for use in gel electrophoresis and western blotting. The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

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Applications
• Monitoring protein migration during SDS-polyacrylamide gel electrophoresis
• Monitoring protein transfer onto membranes after western blotting
• Sizing of proteins on SDS-polyacrylamide gels and western blots

PageRuler™ Unstained Broad Range Protein Ladder (Thermo Scientific™)

Thermo Scientific PageRuler Unstained Broad Range Protein Ladder is a mixture of 11 proteins (5 to 250 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and western blotting. The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

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Product features
• Reference bands—the 100, 50, and 20 kDa bands are more intense for easy orientation
• Tagged— each protein in the contains an integral Strep-tag™ II sequence and can be detected on western blots using Strep-Tactin™ conjugates or an antibody against Strep-tag™ II sequence

Applications
• Accurate protein sizing on SDS-polyacrylamide gels and western blots

PeppermintStick™ Phosphoprotein Molecular Weight Standards (Invitrogen™)

PeppermintStick Phosphoprotein Molecular Weight Standards are a mixture of phosphorylated and nonphosphorylated proteins ranging from 14.4 to 116 kDa. Separation by polyacrylamide gel electrophoresis resolves the standard mixture into two phosphorylated and four nonphosphorylated protein bands.

Applications
• Accurate protein sizing on SDS-polyacrylamide gels and western blots.
• Positive and negative controls for detection of phosphorylated proteins, such as the methods provided by our Pro-Q® Diamond phosphoprotein gel stain

PageRuler™ Prestained NIR Protein Ladder (Thermo Scientific™)

Thermo Scientific PageRuler Prestained NIR Protein Ladder is a mixture of 10 proteins (11 to 250 kDa) that are blue-stained and fluor-labeled for near-IR fluorescent visualization and protein sizing following electrophoresis. The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

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Product features
• Fluorescent—detect using 670 nm red laser or 700 nm channels with fluorescence imagers
• Reference band—55 kDa band has greater intensity for easy orientation

Applications
• Monitoring protein migration during SDS-polyacrylamide gel electrophoresis
• Monitoring protein transfer onto membranes after western blotting
• Sizing of proteins on NIR fluorescent SDS-polyacrylamide gels and western blots

BenchMark™ His-tagged Protein Standard (Invitrogen™)

BenchMark His-tagged Protein Standard is designed as a positive control and for molecular weight sizing in his-tagged fusion protein detection. Each of the ten standard proteins (10-160 kDa) contains a 6X His-tag, enabling the detection of standard proteins with the InVision™ His-tag In-gel Stain (Cat. no. LC6030) or with Anti-His(C-term) Antibody (Cat. no. R930-25) using western detection. The protein standard is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

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Applications
• Verification of His-tagged fusion protein detection using InVision In-Gel Stain or an Anti-His(C-term) antibody
• Sizing of proteins on SDS-polyacrylamide gels and western blots

PageRuler™ Prestained Protein Ladder, 10 to 180 kDa (Thermo Scientific™)

Thermo Scientific PageRuler Prestained Protein Ladder is a mixture of 10 blue-, orange-, and green-stained proteins (10 to 180 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and western blotting. The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

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Applications
• Monitoring protein migration during SDS-polyacrylamide gel electrophoresis
• Monitoring protein transfer onto membranes after western blotting
• Sizing of proteins on SDS-polyacrylamide gels and western blots