Shop All Synthetic RNAi Controls

Silencer™ Negative Control No. 4 siRNA (Invitrogen™)

Ambion® Silencer® Negative Control #4 siRNA has no significant sequence similarity to mouse, rat, or human gene sequences. The control has also been tested in cell-based screens and proved to have no significant effect on cell proliferation, viability, or morphology. 5 nmol is provided.

• Nontargeting with limited sequence similarity to known genes
• Validated for use in human, mouse, and rat cells
• Functionally proven to have minimal effects on cell proliferation and viability
• HPLC purified, duplexed, and ready to use

Negative control siRNAs are essential for determining transfection efficiency and to control for the effects of siRNA delivery. In siRNA screening applications, negative control siRNAs are also critical for setting the "hit" threshold that determines whether an siRNA is considered to have a positive, negative, or neutral effect in a particular assay.

Accessory Product: Silencer® siRNA Screening Control Panel (SKU#AM4640)
The Silencer® siRNA Screening Control Panel includes seven negative control siRNAs and is for those researchers performing siRNA screens that employ hundreds or thousands of siRNAs per experiment. Because negative control siRNAs may have unintended effects in a particular assay, a negative control siRNA should be carefully chosen. The Silencer® siRNA Screening Control Panel simplifies this process. As an added benefit, a positive control siRNA to human, mouse, and rat KIF11 (Eg5) is included in the panel. Knockdown of KIF11, which encodes a kinesin family motor protein, leads to mitotic arrest. Silencer® control siRNAs are unmodified 21-mers; they are designed to be used with Silencer® siRNAs, BLOCK-iT™ siRNAs, and other unmodified 21mer siRNAs. If you are using other siRNA products, such as Ambion® Silencer® Select siRNAs or STEALTH RNAi™ siRNAs, we recommend that you use control siRNAs designed for use with those siRNAs for more reliable experimental results.

Silencer™ GAPDH siRNA (human) (Invitrogen™)

Ambion® Silencer® GAPDH Positive Control siRNA (human) is ideal for developing and optimizing siRNA experiments. The control is validated for use in human cell lines. 5 nmol is provided, along with an additional tube of 2 nmol negative control siRNA.
• Validated siRNA controls for optimizing siRNA experiments
• Gene-specific control siRNAs provided with scrambled, negative controls
• Functionally tested in several common cell lines
• HPLC purified, duplexed, and ready to use Silencer® control siRNAs are unmodified 21-mers; they are designed to be used with Silencer® siRNAs, BLOCK-iT™ siRNAs, and other unmodified 21mer siRNAs. If you are using other siRNA products, such as Ambion® Silencer® Select siRNAs or STEALTH RNAi™ siRNAs, we recommend that you use control siRNAs designed for use with those siRNAs for more reliable experimental results.

Silencer™ Negative Control No. 5 siRNA (Invitrogen™)

Ambion® Silencer® Negative Control #5 siRNA has no significant sequence similarity to mouse, rat, or human gene sequences. The control has also been tested in cell-based screens and proven to have no significant effect on cell proliferation, viability, or morphology. 5 nmol is provided.

• Nontargeting with limited sequence similarity to known genes
• Validated for use in human, mouse, and rat cells
• Functionally proven to have minimal effects on cell proliferation and viability
• HPLC purified, duplexed, and ready to use

Negative control siRNAs are essential for determining transfection efficiency and to control for the effects of siRNA delivery. In siRNA screening applications, negative control siRNAs are also critical for setting the "hit" threshold that determines whether an siRNA is considered to have a positive, negative, or neutral effect in a particular assay.

Accessory Product: Silencer® siRNA Screening Control Panel (SKU#AM4640)
The Silencer® siRNA Screening Control Panel includes seven negative control siRNAs and is for those researchers performing siRNA screens that employ hundreds or thousands of siRNAs per experiment. Because negative control siRNAs may have unintended effects in a particular assay, a negative control siRNA should be carefully chosen. The Silencer® siRNA Screening Control Panel simplifies this process. As an added benefit, a positive control siRNA to human, mouse, and rat KIF11 (Eg5) is included in the panel. Knockdown of KIF11, which encodes a kinesin family motor protein, leads to mitotic arrest. Silencer® control siRNAs are unmodified 21-mers; they are designed to be used with Silencer® siRNAs, BLOCK-iT™ siRNAs, and other unmodified 21mer siRNAs. If you are using other siRNA products, such as Ambion® Silencer® Select siRNAs or STEALTH RNAi™ siRNAs, we recommend that you use control siRNAs designed for use with those siRNAs for more reliable experimental results.

Silencer™ Select Negative Control No. 1 siRNA, in vivo ready (Invitrogen™)

Ambion® Silencer® Select Negative Control siRNA, In Vivo Ready is extensively validated and an ideal control for many aspects of an siRNA experiment. The control is highly purified as required for animal use. 250 nmol is provided.

• Premium-quality siRNA, highly purified and ready to use
• Functionally tested in several common cell lines
• Include Silencer® Select modifications for enhanced specificity
• For use in human, mouse, and rat cells

What are Silencer® Select siRNAs?
Silencer® Select siRNAs incorporate the latest improvements in siRNA design, off-target effect prediction algorithms, and chemical modifications to yield siRNAs with unrivalled efficacy, potency, and specificity. The result is fewer failed experiments due to poor silencing, and cleaner, more consistent phenotypic data.

Silencer® Select GAPDH Positive Control siRNA:
The Silencer® SelectGAPDH positive control is extensively validated and an ideal "test" siRNA for those just beginning siRNA experiments, because it is validated to work in multiple cell lines. Because it targets GAPDH mRNA (a common internal control), many assays are available to measure its effects, including real-time RT-PCR as well as the Ambion® KDalert™ GAPDH Assay Kit.

Quality Control:
Silencer® Select siRNAs are manufactured in a state-of-the-art facility under the highest quality standards. Rigorous quality control procedures include MALDI-TOF mass spectrometry analysis and analytical HPLC to monitor purity. Each siRNA is also assessed by gel electrophoresis to confirm that the strands anneal properly. Furthermore, In Vivo Ready siRNAs undergo additional purification with a semipermeable membrane to remove excess salt. This is followed by sterile filtratation and endotoxin testing. At concentrations of 50 µM in deionized water, In Vivo Ready siRNAs contain. <0.6 mM Na+, <2.0 mM K+, and <0.1 mM Mg2+. Silencer® Select siRNA controls have specific chemical modifications; they are designed to be used with Silencer® Select siRNAs. If you are using other siRNA products, such as BLOCK-iT™ siRNAs, Ambion® Silencer® siRNAs, or STEALTH RNAi™ siRNAs, we recommend that you use control siRNAs designed for use with those siRNAs for more reliable experimental results.

mirVana™ miRNA Mimic, Negative Control #1 (Invitrogen™)

mirVana™ miRNA Mimic Negative Control #1 is designed for use as a negative control for experiments using mirVana™ miRNA Mimics. miRNA mimics are small, chemically modified, double-stranded RNA molecules designed to specifically bind to and mimic endogenous miRNA molecules and enable miRNA functional analysis by up-regulation of miRNA activity. We have now released the second generation of miRNA mimics, mirVana™ miRNA Mimics, which:

• Exhibit maximum and consistent effects in vitro at low concentration
• Are nontoxic
• Offer superior specificity due to unique Star-strand modification
• Target the most updated Sanger content
• Can be used in vitro and in vivo, offering you consistency throughout your entire research project

mirVana™ miRNA Mimic Negative Control #1
mirVana™ miRNA Mimic Negative Control #1 is a random sequence miRNA mimic molecule that has been extensively tested in human cell lines and tissues and validated to not produce identifiable effects on known miRNA function. Transfect the Negative Control using the same methodology as for your positive control (such as miR-1 Positive Control) and experimental mirVana™ miRNA Mimics. Use target gene expression from Negative Control-transfected samples as a baseline for evaluation of the effect of the control and experimental miRNA mimic on target gene expression.

First-generation mimic controls
Our first-generation miRNA mimics (and controls), Pre-miR™ miRNA Precursors, have lower specificity and potency, plus a lower strand specificity compared to the new mirVana™ miRNA Mimics. These first-generation mimics are recommended for completing ongoing in vitro miRNA studies. For new studies or in vivo studies, we highly recommend our new mirVana™ miRNA Mimics and their corresponding controls.

Silencer™ Negative Control No. 1 siRNA (Invitrogen™)

Ambion® Silencer® Negative Control #1 siRNA has no significant sequence similarity to mouse, rat, or human gene sequences. The control has also been tested in cell-based screens and proven to have no significant effect on cell proliferation, viability, or morphology. It is provided in 5 tubes each containing 40 nmol.

• Nontargeting with limited sequence similarity to known genes
• Validated for use in human, mouse, and rat cells
• Functionally proven to have minimal effects on cell proliferation and viability
• HPLC purified, duplexed, and ready to use

Negative control siRNAs are essential for determining transfection efficiency and to control for the effects of siRNA delivery. In siRNA screening applications, negative control siRNAs are also critical for setting the "hit" threshold that determines whether an siRNA is considered to have a positive, negative, or neutral effect in a particular assay.

Accessory Product: Silencer® siRNA Screening Control Panel (SKU#AM4640)
The Silencer® siRNA Screening Control Panel includes seven negative control siRNAs and is for those researchers performing siRNA screens that employ hundreds or thousands of siRNAs per experiment. Because negative control siRNAs may have unintended effects in a particular assay, a negative control siRNA should be carefully chosen. The Silencer® siRNA Screening Control Panel simplifies this process. As an added benefit, a positive control siRNA to human, mouse, and rat KIF11 (Eg5) is included in the panel. Knockdown of KIF11, which encodes a kinesin family motor protein, leads to mitotic arrest. Silencer® control siRNAs are unmodified 21-mers; they are designed to be used with Silencer® siRNAs, BLOCK-iT™ siRNAs, and other unmodified 21mer siRNAs. If you are using other siRNA products, such as Ambion® Silencer® Select siRNAs or STEALTH RNAi™ siRNAs, we recommend that you use control siRNAs designed for use with those siRNAs for more reliable experimental results.

Silencer™ GAPDH siRNA (human, mouse, rat) (Invitrogen™)

Ambion® Silencer® GAPDH Positive Control siRNA (human, mouse, rat) is ideal for developing and optimizing siRNA experiments. The control is validated for use in human, mouse, and rat cell lines. 5 tubes of 40 nmol each are provided.
• Validated siRNA controls for optimizing siRNA experiments
• Functionally tested in several common cell lines
• HPLC purified, duplexed, and ready to use Silencer® control siRNAs are unmodified 21-mers; they are designed to be used with Silencer® siRNAs, BLOCK-iT™ siRNAs, and other unmodified 21mer siRNAs. If you are using other siRNA products, such as Ambion® Silencer® Select siRNAs or STEALTH RNAi™ siRNAs, we recommend that you use control siRNAs designed for use with those siRNAs for more reliable experimental results.

Anti-miR™ miRNA Inhibitor Negative Control #1 (Invitrogen™)

Ambion® Anti-miR™ miRNA Inhibitors are chemically modified, single-stranded nucleic acids designed to specifically bind to and inhibit endogenous microRNA (miRNA) molecules. These ready-to-use inhibitors can be introduced into cells using transfection or electroporation parameters similar to those used for siRNAs. Anti-miR™ Negative Control #1 is a random sequence Anti-miR molecule that has been extensively tested in human cell lines and tissues and validated to produce no identifiable effects on known miRNA function.

Accessory Products:
Also available are Anti-miR™ miRNA Inhibitors (SKU #AM17000 and #AM17001). To select your miRNA sequence and order, please visit the GeneAssist™ miRNA Workflow Builder. Cy™3 and FAM™ dye-labeled Anti-miR™ Negative Controls (SKU #AM171011 and #AM17012) are also available for monitoring transfection efficiency in transfection experiments using Anti-miR miRNA Inhibitors. The Pre-miR™ miRNA Starter Kit (SKU #AM1540) can be used to optimize transfection of Pre-miR™ miRNA Precursors.

Silencer™ Select Negative Control No. 1 siRNA (Invitrogen™)

The Silencer® Select Negative Control No. 1 siRNA is a non-targeting negative control siRNA with the same chemical modifications for enhanced efficacy as in other Silencer® Select siRNAs. Properties of Silencer® Select control siRNAs:

• Validated siRNA controls for optimizing siRNA experiments
• GAPDH Positive Control functionally tested in several common cell lines
• Negative Controls functionally proven to have minimal effects on cell proliferation and viability
• Include Silencer® Select modifications for enhanced specificity
• For use in human, mouse, and rat cells

What are Silencer® Select siRNAs?
Silencer®Select siRNAs are our next-generation siRNAs. These siRNAs incorporate the latest improvements in siRNA design, off-target effect prediction algorithms, and chemical modifications to yield siRNAs with unrivalled efficacy, potency, and specificity. The result is fewer failed experiments due to poor silencing, and cleaner, more consistent phenotypic data.

Silencer® Select Negative Control siRNAs
Negative control siRNAs—siRNAs with sequences that do not target any gene product—are essential for determining the effects of siRNA delivery and for providing a baseline to compare siRNA-treated samples. We have designed and extensively tested two Silencer® Select Negative Control siRNAs. These siRNAs include the same modifications for reducing off-target effects as found in other Silencer® Select siRNAs and have no significant sequence similarity to mouse, rat, or human gene sequences. These negative control siRNAs have been tested by microarray analysis and shown to have minimal effects on gene expression. In addition, these two controls have been tested in multi-parametric, cell-based assays and are proven to have no significant effect on cell proliferation, viability, or morphology in the cell lines tested.

Quality control
We synthesize and purify each Silencer® Select siRNA in state-of-the-art facilities to meet the highest-quality standards. As part of our rigorous quality control procedures, each RNA oligonucleotide is analyzed by MALDI-TOF mass spectrometry, and analytical HPLC is used to monitor purity. To provide the utmost in quality, we also assess each annealed siRNA by gel electrophoresis to confirm that the strands anneal properly. The result is premium-quality siRNA that is purified and ready to use.

Cy3™ Dye-Labeled Pre-miR Negative Control #1 (Invitrogen™)

Ambion® Cy™3 dye-labeled synthetic miRNA mimic for miRNA functional analysis. 5 nmol is supplied.

• Optimized design results in correct strand utilization by RISC
• Purified and ready to transfect

Ambion® Pre-miR™ miRNA Precursor Molecules are small, chemically modified, double-stranded RNA molecules designed to mimic endogenous mature miRNAs. This ready-to-use miRNA mimic can be introduced into cells using transfection or electroporation parameters similar to those used for siRNAs. They enable detailed study of miRNA biological effects via gain-of-function experiments. The Cy™3 Dye-Labeled Pre-miR™ Negative Control #1 is a random sequence Pre-miR™ molecule that has been extensively tested in human cell lines and tissues and validated to produce no identifiable effects on known miRNA function. It is ideal for monitoring transfection efficiency, as the fluorescent label enables direct observation of the cellular uptake, distribution, and localization of the control. The label is on the 5' end of the molecule, and the oligonucleotide sequence is the same as the Pre-miR™ miRNA Precursor Molecule Negative Control #1 (SKU #AM17110). NOTE: Pre-miR™ miRNA Precursors are not hairpin constructs and should not be confused with pre-miRNAs.

Silencer™ Cy™3-labeled GAPDH siRNA (human, mouse, rat) (Invitrogen™)

Ambion® fluorescently labeled control siRNA for the easy monitoring of siRNA uptake by fluorescence microscopy along with siRNA-induced knockdown. Purified, annealed, and ready to use, the 5 nmol aliquot is supplied in a stable, dried format.

• Fluorescently labeled siRNAs simplify the monitoring of siRNA delivery
• Ideal for correlating siRNA uptake with mRNA knockdown
• Functionally tested in several common cell lines
• HPLC purified, duplexed, and ready to use

Visualize siRNA Uptake with Labeled siRNAs
Fluorescent labeling of siRNAs allows direct observation of their cellular uptake, distribution, and localization. Fluorescently labeled siRNAs are commonly used to monitor transfection efficiency during optimization of transfection conditions. In general, siRNAs are rapidly taken up by and distributed throughout cells in as little as 4 hours.

Correlate siRNA Uptake with Gene Silencing
Studies show that the silencing activity of Silencer® Cy™3 Labeled GAPDH siRNA is not affected by the fluorescent label. Therefore, these siRNAs are ideal for examining both siRNA uptake and siRNA-induced knockdown, and for correlating gene knockdown with transfection efficiency; this is important to know because different transfection reagents interact with siRNAs in different ways (i.e., some reagents may sequester the siRNA, resulting in poor gene knockdown). The Silencer® Cy™3 Labeled GAPDH siRNA has the fluorescein derivative Cy™3 (excitation max. = 547, emission max. = 563 nm) on the 5' end of the sense strand, making it highly fluorescent when excited by the appropriate wavelength of light. Silencer® Cy™3 Labeled GAPDH siRNA is suitable for monitoring siRNA uptake by chemical transfection or electroporation. Cy™3 labeling is recommended over FAM™ labeling when using electroporation, because of its brighter fluorescence signal visible over a broader pH range. Electroporation produces a more diffuse siRNA distribution pattern, resulting in a less intense fluorescence signal than with chemical transfection.

Accessory Products:
Silencer® Cy™3 Labeled GAPDH siRNA (human) (SKU #AM4623) is also available as Silencer® FAM™ Labeled GAPDH siRNA for human (SKU #AM4622) and for human, mouse, and rat (SKU #AM4650). Silencer® control siRNAs are unmodified 21-mers; they are designed to be used with Silencer® siRNAs, BLOCK-iT™ siRNAs, and other unmodified 21mer siRNAs. If you are using other siRNA products, such as Ambion® Silencer® Select siRNAs or STEALTH RNAi™ siRNAs, we recommend that you use control siRNAs designed for use with those siRNAs for more reliable experimental results.

Silencer™ Negative Control No. 1 siRNA (Invitrogen™)

Ambion® Silencer Negative Control #1 siRNA, In Vivo Ready has no significant sequence similarity to mouse, rat, or human gene sequences. The control has also been tested in cell-based screens and proven to have no significant effect on cell proliferation, viability, or morphology. It is highly purified as required for animal use. 250 nmol is provided.

• Nontargeting with limited sequence similarity to known genes
• Validated for use in human, mouse, and rat cells
• Functionally proven to have minimal effects on cell proliferation and viability
• HPLC purified, duplexed, and ready to use

Negative control siRNAs are essential for determining transfection efficiency and to control for the effects of siRNA delivery. In siRNA screening applications, negative control siRNAs are also critical for setting the "hit" threshold that determines whether an siRNA is considered to have a positive, negative, or neutral effect in a particular assay. Silencer® Negative Control #1 siRNA has no significant sequence similarity to mouse, rat, or human gene sequences. The control has also been tested in cell-based screens and proven to have no significant effect on cell proliferation, viability, or morphology.

Quality Control:
Silencer® siRNAs are manufactured in a state-of-the-art facility under the highest quality standards. After HPLC purification and annealing, each siRNA is further purified utilizing a process that removes excess salt via a semipermeable membrane. The result is highly pure siRNA with minimal salt content, suitable for in vivo applications. In Vivo Ready siRNAs are then sterile filtered and tested for the presence of endotoxin. At concentrations of 50 µM in deionized water, In Vivo Ready siRNAs contain <0.6 mM Na+, <2.0 mM K+, and <0.1 mM Mg2+.

Accessory Product: Silencer® siRNA Screening Control Panel (SKU#AM4640)
The Silencer® siRNA Screening Control Panel includes seven negative control siRNAs, and is for those researchers performing siRNA screens that employ hundreds or thousands of siRNAs per experiment. Because negative control siRNAs may have unintended effects in a particular assay, a negative control siRNA should be carefully chosen. The Silencer® siRNA Screening Control Panel simplifies this process. As an added benefit, a positive control siRNA to human, mouse, and rat KIF11 (Eg5) is included in the panel. Knockdown of KIF11, which encodes a kinesin family motor protein, leads to mitotic arrest. Silencer® control siRNAs are unmodified 21-mers; they are designed to be used with Silencer® siRNAs, BLOCK-iT™ siRNAs, and other unmodified 21mer siRNAs. If you are using other siRNA products, such as Ambion® Silencer® Select siRNAs or STEALTH RNAi™ siRNAs, we recommend that you use control siRNAs designed for use with those siRNAs for more reliable experimental results.

BLOCK-iT™ Transfection Optimization Kit (Invitrogen™)

The BLOCK-iT™ Transfection Optimization Kit is designed to optimize RNAi transfection using controls for transfection and viability. Each kit contains a BLOCK-iT™ Fluorescent Oligo, which provides:

• A strong, easily detectable FITC signal indicating transfection efficiency
• Chemical modifications for a clearer, more persistent cellular signal than labeled siRNA provides
• Proven correlation of transfection efficiency with Stealth RNAi™ siRNA and traditional siRNA molecules

This makes BLOCK-iT™ Fluorescent Oligo an ideal indicator of transfection efficiency for RNAi experiments. In addition, the kit contains a Positive Control Stealth RNAi™ siRNA molecule directed at human p53 and a Scrambled Stealth RNAi™ siRNA molecule as a negative control. To assay cell viability, Dead Cell Dye is included. This dye colors dead cells as red, thereby allowing easy identification of non-viable cells in a tissue culture plate. Using these reagents together allows you to both optimize transfection conditions and have a functional positive control in every RNAi experiment you perform.

BLOCK-iT™ Fluorescent Oligo, for lipid transfection

BLOCK-iT™ Fluorescent Oligo provides:

• Strong, easily detectable FITC signal indicating transfection efficiency
Chemical modifications for a clearer, more persistent cellular signal than labeled siRNA
Proven correlation of transfection efficiency with Stealth RNAi™ siRNA and traditional siRNA molecules

This makes the BLOCK-iT™ Fluorescent Oligo an ideal indicator of transfection efficiency for RNAi experiments. BLOCK-iT™ Fluorescent Oligo is available in two configurations: one designed for lipid-mediated transfection (2 x 125 µL) at 20 µM and one designed for electroporation (75 µl) at 1 mM.

Silencer™ Negative Control No. 2 siRNA (Invitrogen™)

Ambion® Silencer® Negative Control #2 siRNA has no significant sequence similarity to mouse, rat, or human gene sequences. The control has also been tested in cell-based screens and proven to have no significant effect on cell proliferation, viability, or morphology. 40 nmol is provided.

• Nontargeting with limited sequence similarity to known genes
• Validated for use in human, mouse, and rat cells
• Functionally proven to have minimal effects on cell proliferation and viability
• HPLC purified, duplexed, and ready to use

Negative control siRNAs are essential for determining transfection efficiency and to control for the effects of siRNA delivery. In siRNA screening applications, negative control siRNAs are also critical for setting the "hit" threshold that determines whether an siRNA is considered to have a positive, negative, or neutral effect in a particular assay.

Accessory Product: Silencer® siRNA Screening Control Panel (SKU#AM4640)
The Silencer® siRNA Screening Control Panel includes seven negative control siRNAs and is for those researchers performing siRNA screens that employ hundreds or thousands of siRNAs per experiment. Because negative control siRNAs may have unintended effects in a particular assay, a negative control siRNA should be carefully chosen. The Silencer® siRNA Screening Control Panel simplifies this process. As an added benefit, a positive control siRNA to human, mouse, and rat KIF11 (Eg5) is included in the panel. Knockdown of KIF11, which encodes a kinesin family motor protein, leads to mitotic arrest. Silencer® control siRNAs are unmodified 21-mers; they are designed to be used with Silencer® siRNAs, BLOCK-iT™ siRNAs, and other unmodified 21mer siRNAs. If you are using other siRNA products, such as Ambion® Silencer® Select siRNAs or STEALTH RNAi™ siRNAs, we recommend that you use control siRNAs designed for use with those siRNAs for more reliable experimental results.