Shop All RNAi Negative Controls

Silencer™ Select Negative Control No. 1 siRNA (Invitrogen™)

The Silencer® Select Negative Control No. 1 siRNA is a non-targeting negative control siRNA with the same chemical modifications for enhanced efficacy as in other Silencer® Select siRNAs. Properties of Silencer® Select control siRNAs:

• Validated siRNA controls for optimizing siRNA experiments
• GAPDH Positive Control functionally tested in several common cell lines
• Negative Controls functionally proven to have minimal effects on cell proliferation and viability
• Include Silencer® Select modifications for enhanced specificity
• For use in human, mouse, and rat cells

What are Silencer® Select siRNAs?
Silencer®Select siRNAs are our next-generation siRNAs. These siRNAs incorporate the latest improvements in siRNA design, off-target effect prediction algorithms, and chemical modifications to yield siRNAs with unrivalled efficacy, potency, and specificity. The result is fewer failed experiments due to poor silencing, and cleaner, more consistent phenotypic data.

Silencer® Select Negative Control siRNAs
Negative control siRNAs—siRNAs with sequences that do not target any gene product—are essential for determining the effects of siRNA delivery and for providing a baseline to compare siRNA-treated samples. We have designed and extensively tested two Silencer® Select Negative Control siRNAs. These siRNAs include the same modifications for reducing off-target effects as found in other Silencer® Select siRNAs and have no significant sequence similarity to mouse, rat, or human gene sequences. These negative control siRNAs have been tested by microarray analysis and shown to have minimal effects on gene expression. In addition, these two controls have been tested in multi-parametric, cell-based assays and are proven to have no significant effect on cell proliferation, viability, or morphology in the cell lines tested.

Quality control
We synthesize and purify each Silencer® Select siRNA in state-of-the-art facilities to meet the highest-quality standards. As part of our rigorous quality control procedures, each RNA oligonucleotide is analyzed by MALDI-TOF mass spectrometry, and analytical HPLC is used to monitor purity. To provide the utmost in quality, we also assess each annealed siRNA by gel electrophoresis to confirm that the strands anneal properly. The result is premium-quality siRNA that is purified and ready to use.

Ambion™ In Vivo Negative Control #1 siRNA (Invitrogen™)

Ambion® In Vivo siRNA Controls
• Validated siRNA controls for optimizing siRNA experiments targeting coding and noncoding transcripts
• Positive Control siRNAs functionally tested in several common cell lines
• Negative Controls functionally proven to have minimal effects on cell proliferation and viability
• Include Silencer® Select modifications for enhanced specificity or Ambion® In Vivo modifications for increased stability
• For use in human, mouse, and rat cells

What are Ambion® In Vivo siRNAs?
Ambion® In Vivo siRNAs are designed using the proven Silencer® Select algorithm and incorporate chemical modifications for superior serum stability with in vivo delivery. The added serum stability does not compromise the performance of the siRNAs. Ambion® In Vivo siRNAs have been shown to be non-toxic in vivo (mouse) and non-immunogenic (cell-based assays). In cell-based assays, Ambion® In Vivo siRNAs exhibit equivalent or better potency than Silencer® Select siRNAs. The result is better confidence in your in vivo RNAi experiments, better knockdown when paired with an efficient delivery solution, and the assurance of limited non-specific effects.

Negative Control siRNAs
Negative control siRNAs—siRNAs with sequences that do not target any gene product—are essential for determining the effects of siRNA delivery and for providing a baseline to compare siRNA-treated samples. Ambion® In Vivo siRNAs and have no significant sequence similarity to mouse, rat, or human gene sequences. These negative control siRNAs have been tested by microarray analysis and shown to have minimal effects on gene expression. In addition, these negative control siRNA has been tested in multi-parametric cell-based assays and are proven to have no significant effect on cell proliferation, viability, or morphology in the cell lines tested.

Quality Control
Ambion® synthesizes and purifies each Ambion® In Vivo siRNA in state-of-the-art facilities to meet the highest quality standards. As part of our rigorous quality control procedures, each RNA oligonucleotide is analyzed by MALDI-TOF mass spectrometry, and analytical HPLC is used to monitor purity. To provide the utmost in quality, we also assess each annealed siRNA by gel electrophoresis to confirm that the strands anneal properly. The result is premium-quality siRNA that is purified and ready to use.

In Vivo Ready Controls
Our "In Vivo Ready" Control siRNAs are subjected to that extra level of purification and testing required for the introduction of siRNAs into animals. After HPLC purification and annealing, each siRNA is further purified utilizing a process that removes excess salt via a semi-permeable membrane. The result is highly pure siRNA with minimal salt content, suitable for in vivo applications. In vivo siRNAs are then sterile filtered, and tested for the presence of endotoxin. At concentrations of 50 µM in presence of deionized water, in vivo Ready siRNAs contain <5.0 mM Na+, <0.06 mM K+, and <0.02 mM Mg2+.

Stealth RNAi™ siRNA Negative Control Hi GC (Invitrogen™)

Stealth RNAi™ siRNA Negative Controls are a great way to measure the effect of your experimental Stealth RNAi™ siRNA duplex versus background. These controls are: –Designed in 3 levels of GC content (Lo, Med, Hi) with 3 sequences available per level so researchers can match GC content to their experimental Stealth RNAi™ siRNA –Not homologous to anything in vertebrate transcriptome –Tested not to induce stress response Stealth RNAi™ siRNA Negative Control Kit contains all three controls (Lo, Med, Hi GC content-excluding sequences #2 and #3) and each is also available separately. Get the ideal negative controls for your RNAi experiment so you can assess your results accurately.

Silencer™ Negative Control No. 2 siRNA (Invitrogen™)

Ambion® Silencer® Negative Control #2 siRNA has no significant sequence similarity to mouse, rat, or human gene sequences. The control has also been tested in cell-based screens and proven to have no significant effect on cell proliferation, viability, or morphology. 40 nmol is provided.

• Nontargeting with limited sequence similarity to known genes
• Validated for use in human, mouse, and rat cells
• Functionally proven to have minimal effects on cell proliferation and viability
• HPLC purified, duplexed, and ready to use

Negative control siRNAs are essential for determining transfection efficiency and to control for the effects of siRNA delivery. In siRNA screening applications, negative control siRNAs are also critical for setting the "hit" threshold that determines whether an siRNA is considered to have a positive, negative, or neutral effect in a particular assay.

Accessory Product: Silencer® siRNA Screening Control Panel (SKU#AM4640)
The Silencer® siRNA Screening Control Panel includes seven negative control siRNAs and is for those researchers performing siRNA screens that employ hundreds or thousands of siRNAs per experiment. Because negative control siRNAs may have unintended effects in a particular assay, a negative control siRNA should be carefully chosen. The Silencer® siRNA Screening Control Panel simplifies this process. As an added benefit, a positive control siRNA to human, mouse, and rat KIF11 (Eg5) is included in the panel. Knockdown of KIF11, which encodes a kinesin family motor protein, leads to mitotic arrest. Silencer® control siRNAs are unmodified 21-mers; they are designed to be used with Silencer® siRNAs, BLOCK-iT™ siRNAs, and other unmodified 21mer siRNAs. If you are using other siRNA products, such as Ambion® Silencer® Select siRNAs or STEALTH RNAi™ siRNAs, we recommend that you use control siRNAs designed for use with those siRNAs for more reliable experimental results.

FAM™ Dye-Labeled Anti-miR™ Negative Control #1 (Invitrogen™)

A FAM™ dye–labeled synthetic miRNA inhibitor for miRNA functional analysis. 5 nmol is supplied, purified and ready to transfect. Ambion® Anti-miR™ miRNA Inhibitors are chemically modified, single-stranded nucleic acids designed to specifically bind to and inhibit endogenous microRNA (miRNA) molecules. These ready-to-use inhibitors can be introduced into cells using transfection or electroporation parameters similar to those used for siRNAs. FAM™ Dye Labeled Anti-miR™ Negative Control #1 is a random sequence Anti-miR molecule that has been extensively tested in human cell lines and tissues and validated to produce no identifiable effects on known miRNA function. It is used for monitoring transfection efficiency in transfection experiments using Anti-miR miRNA Inhibitors. The fluorescent label enables direct observation of cellular uptake, distribution, and localization of the control. The molecule is labeled at its 5' end and has the same oligonucleotide sequence as unlabeled Anti-miR™ miRNA Inhibitor Negative Control #1 (SKU #AM17010).

Accessory Products:
Also available are Anti-miR™ miRNA Inhibitors (SKU #AM17000 and #AM17001). To select your miRNA sequence and order, please visit the GeneAssist™ miRNA Workflow Builder. Cy™3 dye-labeled Anti-miR™ Negative Controls (SKU #AM17011) are available. The Pre-miR™ miRNA Starter Kit (SKU #AM1540) can be used to optimize transfection of Pre-miR™ miRNA Precursors.

Silencer™ Select Negative Control No. 2 siRNA (Invitrogen™)

Ambion® Silencer® SelectNegative Control #2 siRNA is extensively validated and an ideal control for many aspects of an siRNA experiment. 5 nmol is provided.

• Premium-quality siRNA, highly purified and ready to use
• Functionally proven to have minimal effects on cell proliferation and viability
• Include Silencer® Select modifications for enhanced specificity
• For use in human, mouse, and rat cells

What are Silencer® Select siRNAs?
Silencer® Select siRNAs incorporate the latest improvements in siRNA design, off-target effect prediction algorithms, and chemical modifications, to yield siRNAs with unrivalled efficacy, potency, and specificity. The result is fewer failed experiments due to poor silencing, and cleaner, more consistent phenotypic data.

Silencer® Select Negative Control #2 siRNA:
Negative control siRNAs—siRNAs with sequences that do not target any gene product—are essential for determining the effects of siRNA delivery and for providing a baseline to compare siRNA-treated samples. Silencer® Select Negative Control #2 siRNA has been carefully designed and extensively tested, and has no significant sequence similarity to mouse, rat, or human gene sequences. This negative control siRNA has been tested by microarray analysis and shown to have minimal effects on gene expression. It has also been tested in multi-parametric, cell-based assays and shown to have no significant effect on cell proliferation, viability, or morphology in the cell lines tested.

Quality Control:
Silencer® Select siRNAs are manufactured in a state-of-the-art facility under the highest quality standards. Rigorous quality control procedures include MALDI-TOF mass spectrometry analysis and analytical HPLC to monitor purity. Each siRNA is also assessed by gel electrophoresis to confirm that the strands anneal properly.

Accessory Product: siPORT™ NeoFX™ Transfection Agent (SKU #AM4510 and #AM4511)
siPORT™ NeoFX™ Transfection Agent is a versatile, lipid-based transfection agent for the efficient transfection of siRNA into cultured cells. The protocol can be adapted to a wide range of cells and experimental designs, including high-throughput applications. Silencer® Select siRNA controls have specific chemical modifications; they are designed to be used with Silencer® Select siRNAs. If you are using other siRNA products, such as BLOCK-iT™ siRNAs, Ambion® Silencer® siRNAs, or STEALTH RNAi™ siRNAs, we recommend that you use control siRNAs designed for use with those siRNAs for more reliable experimental results.

Stealth RNAi™ siRNA Negative Control Hi GC Duplex #2 (Invitrogen™)

Stealth RNAi™ siRNA Negative Controls are a great way to measure the effect of your experimental Stealth RNAi™ siRNA duplex versus background. These controls are:

• Designed in 3 levels of GC content (Lo, Med, Hi) with 3 sequences available per level so researchers can match GC content to their experimental Stealth RNAi™ siRNA
• Not homologous to anything in the vertebrate transcriptome
• Tested not to induce stress response Stealth RNAi™ siRNA

Negative Control Kit contains all three controls (Lo, Med, Hi GC content-excluding sequences #2 and #3) and each is also available separately. Get the ideal negative controls for your RNAi experiment so you can assess your results accurately.

mirVana™ miRNA Inhibitor, Negative Control #1 (Invitrogen™)

miRNA inhibitors are small, chemically modified single-stranded RNA molecules designed to specifically bind to and inhibit endogenous miRNA molecules and enable miRNA functional analysis by down-regulation of miRNA activity.

We have now released the second generation of miRNA inhibitors, mirVana™ miRNA Inhibitors, which:
Display better potency than lead competitor's product
• Offer maximum and consistent effect in vitro at low miRNA inhibitor concentration
• Are non toxic
• Target the most updated Sanger content
• Can be used in vitro and in vivo, offering consistency throughout your entire research project


Positive and negative controls are also available for use along with mirVana™ miRNA Inhibitors in miRNA down-regulation experiments.

mirVana™ miRNA Inhibitor Negative Control #1
mirVana™ miRNA Inhibitor Negative Control #1 is intended for use as a negative control for experiments using mirVana™ miRNA Inhibitors. Transfect this negative control using the same methodology as for your positive control (such as let-7c Positive Control) and experimental mirVana™ miRNA Inhibitors. Use target expression from negative control-transfected samples as a baseline for evaluation of the effect of the control and experimental miRNA inhibitor on target gene expression.

First Generation Inhibitor Controls
Our first-generation miRNA inhibitors (and controls), Ambion® Anti-miR™ miRNA Inhibitors, have lower specificity and potency, plus a lower strand specificity compared to the new mirVana™ miRNA Inhibitors. These first-generation inhibitors are recommended for completing ongoing in vitro miRNA studies. For new studies or in vivo studies, we highly recommend our new mirVana™ miRNA Inhibitors and their corresponding controls.

Silencer™ FAM-labeled Negative Control No. 1 siRNA (Invitrogen™)

Ambion® A fluorescently labeled negative control siRNA for the easy monitoring of siRNA uptake by fluorescence microscopy. Purified, annealed, and ready to use, the 5 nmol aliquot is supplied in a stable, dried format.

• Simplifies the monitoring of siRNA delivery
• Functionally tested in several common cell lines
• HPLC purified, duplexed, and ready to use

Visualize siRNA Uptake with Labeled siRNAs
Fluorescent labeled siRNAs allow for the direct observation siRNA cellular uptake, distribution, and localization. Fluorescently labeled siRNAs are commonly used to monitor transfection efficiency during optimization of transfection conditions. In general, siRNAs are rapidly taken up by and distributed throughout cells in as little as 4 hours. Fluorescent labeling of siRNAs allows for rapid monitoring of siRNA delivery by fluorescence microscopy, as opposed to unlabeled siRNAs, whose delivery must be monitored by target gene knockdown. Silencer® FAM Labeled Negative Control #1 siRNAs provide an easy way to monitor siRNA uptake by chemical transfection. The sequences is identical to that of the Ambion Silencer® Negative Control# 1 siRNA (SKU #AM4611), but with a fluorescein derivative FAM (5'-carboxyfluorescein; excitation max. = 494 nm, emission max. = 520 nm) on the 5' end of the sense strand, making it highly fluorescent when excited by the appropriate wavelength of light.

Accessory Products:
For the monitoring of siRNA delivery by electroporation, Silencer® Cy™3 Labeled Negative Control #1 siRNA (SKU #AM4621) is recommended because of its brighter fluorescence signal visible over a broader pH range. Electroporation produces a more diffuse siRNA distribution pattern, resulting in a less intense fluorescence signal than with chemical transfection. Silencer® control siRNAs are unmodified 21-mers; they are designed to be used with Silencer® siRNAs, BLOCK-iT™ siRNAs, and other unmodified 21mer siRNAs. If you are using other siRNA products, such as Ambion® Silencer® Select siRNAs or STEALTH RNAi™ siRNAs, we recommend that you use control siRNAs designed for use with those siRNAs for more reliable experimental results.

Stealth RNAi™ siRNA Negative Control Hi GC Duplex #3 (Invitrogen™)

Stealth RNAi™ siRNA Negative Controls are a great way to measure the effect of your experimental Stealth RNAi™ siRNA duplex versus background. These controls are:

• Designed in 3 levels of GC content (Lo, Med, Hi) with 3 sequences available per level so researchers can match GC content to their experimental Stealth RNAi™ siRNA
• Not homologous to anything in the vertebrate transcriptome
• Tested not to induce stress response Stealth RNAi™ siRNA

Negative Control Kit contains all three controls (Lo, Med, Hi GC content-excluding sequences #2 and #3) and each is also available separately. Get the ideal negative controls for your RNAi experiment so you can assess your results accurately.

Pre-miR™ miRNA Precursor Molecules—Negative Control #2 (Invitrogen™)

A synthetic miRNA mimic for miRNA functional analysis. 5 nmol is supplied.

• Optimized design results in correct strand utilization by RISC
• Purified and ready to transfect

Ambion® Pre-miR™ miRNA Precursor Molecules are small, chemically modified, double-stranded RNA molecules designed to mimic endogenous mature miRNAs. This ready-to-use miRNA mimic can be introduced into cells using transfection or electroporation parameters similar to those used for siRNAs. They enable detailed study of miRNA biological effects via gain-of-function experiments. Pre-miR™ Negative Control #2 is a random sequence Pre-miR™ molecule that has been extensively tested in human cell lines and tissues and validated to produce no identifiable effects on known miRNA function. NOTE: Pre-miR™ miRNA Precursors are not hairpin constructs and should not be confused with pre-miRNAs.

Silencer™ Negative Control No. 1 siRNA (Invitrogen™)

Ambion® Silencer® Negative Control #1 siRNA has no significant sequence similarity to mouse, rat, or human gene sequences. The control has also been tested in cell-based screens and proven to have no significant effect on cell proliferation, viability, or morphology. 5 nmol is provided.

• Nontargeting with limited sequence similarity to known genes
• Validated for use in human, mouse, and rat cells
• Functionally proven to have minimal effects on cell proliferation and viability
• HPLC purified, duplexed, and ready to use

Negative control siRNAs are essential for determining transfection efficiency and to control for the effects of siRNA delivery. In siRNA screening applications, negative control siRNAs are also critical for setting the "hit" threshold that determines whether an siRNA is considered to have a positive, negative, or neutral effect in a particular assay.

Accessory Product: Silencer® siRNA Screening Control Panel (SKU#AM4640)
The Silencer® siRNA Screening Control Panel includes seven negative control siRNAs and is for those researchers performing siRNA screens that employ hundreds or thousands of siRNAs per experiment. Because negative control siRNAs may have unintended effects in a particular assay, a negative control siRNA should be carefully chosen. The Silencer® siRNA Screening Control Panel simplifies this process. As an added benefit, a positive control siRNA to human, mouse, and rat KIF11 (Eg5) is included in the panel. Knockdown of KIF11, which encodes a kinesin family motor protein, leads to mitotic arrest. Silencer® control siRNAs are unmodified 21-mers; they are designed to be used with Silencer® siRNAs, BLOCK-iT™ siRNAs, and other unmodified 21mer siRNAs. If you are using other siRNA products, such as Ambion® Silencer® Select siRNAs or STEALTH RNAi™ siRNAs, we recommend that you use control siRNAs designed for use with those siRNAs for more reliable experimental results.

Stealth RNAi™ siRNA Negative Control Lo GC Duplex #2 (Invitrogen™)

Stealth RNAi™ siRNA Negative Controls are a great way to measure the effect of your experimental Stealth RNAi™ siRNA duplex versus background. These controls are:

• Designed in 3 levels of GC content (Lo, Med, Hi) with 3 sequences available per level so researchers can match GC content to their experimental Stealth RNAi™ siRNA
• Not homologous to anything in the vertebrate transcriptome
• Tested not to induce stress response Stealth RNAi™ siRNA

Negative Control Kit contains all three controls (Lo, Med, Hi GC content-excluding sequences #2 and #3) and each is also available separately. Get the ideal negative controls for your RNAi experiment so you can assess your results accurately.

mirVana™ miRNA Mimic, Negative Control #1 (Invitrogen™)

mirVana™ miRNA Mimic Negative Control #1 is designed for use as a negative control for experiments using mirVana™ miRNA Mimics. miRNA mimics are small, chemically modified, double-stranded RNA molecules designed to specifically bind to and mimic endogenous miRNA molecules and enable miRNA functional analysis by up-regulation of miRNA activity. We have now released the second generation of miRNA mimics, mirVana™ miRNA Mimics, which:

• Exhibit maximum and consistent effects in vitro at low concentration
• Are nontoxic
• Offer superior specificity due to unique Star-strand modification
• Target the most updated Sanger content
• Can be used in vitro and in vivo, offering you consistency throughout your entire research project

mirVana™ miRNA Mimic Negative Control #1
mirVana™ miRNA Mimic Negative Control #1 is a random sequence miRNA mimic molecule that has been extensively tested in human cell lines and tissues and validated to not produce identifiable effects on known miRNA function. Transfect the Negative Control using the same methodology as for your positive control (such as miR-1 Positive Control) and experimental mirVana™ miRNA Mimics. Use target gene expression from Negative Control-transfected samples as a baseline for evaluation of the effect of the control and experimental miRNA mimic on target gene expression.

First-generation mimic controls
Our first-generation miRNA mimics (and controls), Pre-miR™ miRNA Precursors, have lower specificity and potency, plus a lower strand specificity compared to the new mirVana™ miRNA Mimics. These first-generation mimics are recommended for completing ongoing in vitro miRNA studies. For new studies or in vivo studies, we highly recommend our new mirVana™ miRNA Mimics and their corresponding controls.

Silencer™ Negative Control No. 4 siRNA (Invitrogen™)

Ambion® Silencer® Negative Control #4 siRNA has no significant sequence similarity to mouse, rat, or human gene sequences. The control has also been tested in cell-based screens and proved to have no significant effect on cell proliferation, viability, or morphology. 5 nmol is provided.

• Nontargeting with limited sequence similarity to known genes
• Validated for use in human, mouse, and rat cells
• Functionally proven to have minimal effects on cell proliferation and viability
• HPLC purified, duplexed, and ready to use

Negative control siRNAs are essential for determining transfection efficiency and to control for the effects of siRNA delivery. In siRNA screening applications, negative control siRNAs are also critical for setting the "hit" threshold that determines whether an siRNA is considered to have a positive, negative, or neutral effect in a particular assay.

Accessory Product: Silencer® siRNA Screening Control Panel (SKU#AM4640)
The Silencer® siRNA Screening Control Panel includes seven negative control siRNAs and is for those researchers performing siRNA screens that employ hundreds or thousands of siRNAs per experiment. Because negative control siRNAs may have unintended effects in a particular assay, a negative control siRNA should be carefully chosen. The Silencer® siRNA Screening Control Panel simplifies this process. As an added benefit, a positive control siRNA to human, mouse, and rat KIF11 (Eg5) is included in the panel. Knockdown of KIF11, which encodes a kinesin family motor protein, leads to mitotic arrest. Silencer® control siRNAs are unmodified 21-mers; they are designed to be used with Silencer® siRNAs, BLOCK-iT™ siRNAs, and other unmodified 21mer siRNAs. If you are using other siRNA products, such as Ambion® Silencer® Select siRNAs or STEALTH RNAi™ siRNAs, we recommend that you use control siRNAs designed for use with those siRNAs for more reliable experimental results.