Shop All RNAi Negative Controls

Anti-miR™ miRNA Inhibitor Negative Control #1 Invitrogen™

Ambion® Anti-miR™ miRNA Inhibitors are chemically modified, single-stranded nucleic acids designed to specifically bind to and inhibit endogenous microRNA (miRNA) molecules. These ready-to-use inhibitors can be introduced into cells using transfection or electroporation parameters similar to those used for siRNAs. Anti-miR™ Negative Control #1 is a random sequence Anti-miR molecule that has been extensively tested in human cell lines and tissues and validated to produce no identifiable effects on known miRNA function.

Accessory Products:
Also available are Anti-miR™ miRNA Inhibitors (SKU #AM17000 and #AM17001). To select your miRNA sequence and order, please visit the GeneAssist™ miRNA Workflow Builder. Cy™3 and FAM™ dye-labeled Anti-miR™ Negative Controls (SKU #AM171011 and #AM17012) are also available for monitoring transfection efficiency in transfection experiments using Anti-miR miRNA Inhibitors. The Pre-miR™ miRNA Starter Kit (SKU #AM1540) can be used to optimize transfection of Pre-miR™ miRNA Precursors.

mirVana™ miRNA Mimic, Negative Control #1 Invitrogen™

miRNA mimics are small, chemically modified double-stranded RNA molecules designed to specifically bind to and mimic endogenous miRNA molecules and enable miRNA functional analysis by up-regulation of miRNA activity.

We have now released the second generation of miRNA mimics, mirVana™ miRNA Mimics, which:
Exhibit maximum and consistent effect in vitro at low concentration
• Are non toxic
• Offer superior specificity due to unique Star strand modification
• Target the most updated Sanger content
• Can be used in vitro and in vivo, offering you consistency throughout your entire research project


Positive and negative controls are also available for use along with mirVana™ miRNA Mimics in miRNA up-regulation experiments.

mirVana™ miRNA Mimic Negative Control #1
mirVana™ miRNA Mimic Negative Control #1 is designed to use as a negative control for experiments using mirVana™ miRNA Mimics. Transfect the Negative Control using the same methodology as for your positive control (such as miR-1 Positive Control) and experimental mirVana™ miRNA Mimics. Use target gene expression from Negative Control-transfected samples as a baseline for evaluation of the effect of the control and experimental miRNA mimic on target gene expression.

mirVana™ miRNA Mimic Negative Control #1 is a random sequence miRNA mimic molecule that has been extensively tested in human cell lines and tissues and validated to not produce identifiable effects on known miRNA function.

First Generation Mimic Controls
Our first-generation miRNA mimics (and controls), Ambion® Pre-miR™ miRNA Precursors, have lower specificity and potency, plus a lower strand specificity compared to the new mirVana™ miRNA Mimics. These first-generation mimics are recommended for completing ongoing in vitro miRNA studies. For new studies or in vivo studies, we highly recommend our new mirVana™ miRNA Mimics and their corresponding controls.

Stealth RNAi™ siRNA Negative Control Kit Invitrogen™

Stealth RNAi™ siRNA Negative Controls are a great way to measure the effect of your experimental Stealth RNAi™ siRNA duplex versus background. These controls are:

• Designed in 3 levels of GC content (Lo, Med, Hi) with 3 sequences available per level so researchers can match GC content to their experimental Stealth RNAi™ siRNA
• Not homologous to anything in the vertebrate transcriptome
• Tested not to induce stress response Stealth RNAi™ siRNA

Negative Control Kit contains all three controls (Lo, Med, Hi GC content-excluding sequences #2 and #3) and each is also available separately. Get the ideal negative controls for your RNAi experiment so you can assess your results accurately.

Cy3™ Dye-Labeled Anti-miR™ Negative Control #1 Invitrogen™

Ambion® Cy™3 dye–labeled synthetic miRNA inhibitor for miRNA functional analysis. 5 nmol is supplied, purified and ready to transfect. Ambion® Anti-miR™ miRNA Inhibitors are chemically modified, single-stranded nucleic acids designed to specifically bind to and inhibit endogenous microRNA (miRNA) molecules. These ready-to-use inhibitors can be introduced into cells using transfection or electroporation parameters similar to those used for siRNAs. Cy™3 Dye Labeled Anti-miR™ Negative Control #1 is a random sequence Anti-miR molecule that has been extensively tested in human cell lines and tissues and validated to produce no identifiable effects on known miRNA function. It is used for monitoring transfection efficiency in transfection experiments using Anti-miR miRNA Inhibitors. The fluorescent label enables direct observation of cellular uptake, distribution, and localization of the control. The molecule is labeled at its 5' end and has the same oligonucleotide sequence as unlabeled Anti-miR™ miRNA Inhibitor Negative Control #1 (SKU #AM17010).

Accessory Products:
Also available are Anti-miR™ miRNA Inhibitors (SKU #AM17000 and #AM17001). To select your miRNA sequence and order, please visit the GeneAssist™ miRNA Workflow Builder. FAM™ dye-labeled Anti-miR™ Negative Controls (SKU #AM17012) is available. The Pre-miR™ miRNA Starter Kit (SKU #AM1540) can be used to optimize transfection of Pre-miR™ miRNA Precursors.

Stealth RNAi™ siRNA Negative Control Lo GC Invitrogen™

Stealth RNAi™ siRNA Negative Controls are a great way to measure the effect of your experimental Stealth RNAi™ siRNA duplex versus background. These controls are: –Designed in 3 levels of GC content (Lo, Med, Hi) with 3 sequences available per level so researchers can match GC content to their experimental Stealth RNAi™ siRNA –Not homologous to anything in vertebrate transcriptome –Tested not to induce stress response Stealth RNAi™ siRNA Negative Control Kit contains all three controls (Lo, Med, Hi GC content-excluding sequences #2 and #3) and each is also available separately. Get the ideal negative controls for your RNAi experiment so you can assess your results accurately.

mirVana™ miRNA Inhibitor, Negative Control #1 Invitrogen™

miRNA inhibitors are small, chemically modified single-stranded RNA molecules designed to specifically bind to and inhibit endogenous miRNA molecules and enable miRNA functional analysis by down-regulation of miRNA activity.

We have now released the second generation of miRNA inhibitors, mirVana™ miRNA Inhibitors, which:
Display better potency than lead competitor's product
• Offer maximum and consistent effect in vitro at low miRNA inhibitor concentration
• Are non toxic
• Target the most updated Sanger content
• Can be used in vitro and in vivo, offering consistency throughout your entire research project


Positive and negative controls are also available for use along with mirVana™ miRNA Inhibitors in miRNA down-regulation experiments.

mirVana™ miRNA Inhibitor Negative Control #1
mirVana™ miRNA Inhibitor Negative Control #1 is intended for use as a negative control for experiments using mirVana™ miRNA Inhibitors. Transfect this negative control using the same methodology as for your positive control (such as let-7c Positive Control) and experimental mirVana™ miRNA Inhibitors. Use target expression from negative control-transfected samples as a baseline for evaluation of the effect of the control and experimental miRNA inhibitor on target gene expression.

First Generation Inhibitor Controls
Our first-generation miRNA inhibitors (and controls), Ambion® Anti-miR™ miRNA Inhibitors, have lower specificity and potency, plus a lower strand specificity compared to the new mirVana™ miRNA Inhibitors. These first-generation inhibitors are recommended for completing ongoing in vitro miRNA studies. For new studies or in vivo studies, we highly recommend our new mirVana™ miRNA Inhibitors and their corresponding controls.

Stealth RNAi™ siRNA Negative Control Lo GC Duplex #2 Invitrogen™

Stealth RNAi™ siRNA Negative Controls are a great way to measure the effect of your experimental Stealth RNAi™ siRNA duplex versus background. These controls are:

• Designed in 3 levels of GC content (Lo, Med, Hi) with 3 sequences available per level so researchers can match GC content to their experimental Stealth RNAi™ siRNA
• Not homologous to anything in the vertebrate transcriptome
• Tested not to induce stress response Stealth RNAi™ siRNA

Negative Control Kit contains all three controls (Lo, Med, Hi GC content-excluding sequences #2 and #3) and each is also available separately. Get the ideal negative controls for your RNAi experiment so you can assess your results accurately.

FAM™ Dye-Labeled Anti-miR™ Negative Control #1 Invitrogen™

A FAM™ dye–labeled synthetic miRNA inhibitor for miRNA functional analysis. 5 nmol is supplied, purified and ready to transfect. Ambion® Anti-miR™ miRNA Inhibitors are chemically modified, single-stranded nucleic acids designed to specifically bind to and inhibit endogenous microRNA (miRNA) molecules. These ready-to-use inhibitors can be introduced into cells using transfection or electroporation parameters similar to those used for siRNAs. FAM™ Dye Labeled Anti-miR™ Negative Control #1 is a random sequence Anti-miR molecule that has been extensively tested in human cell lines and tissues and validated to produce no identifiable effects on known miRNA function. It is used for monitoring transfection efficiency in transfection experiments using Anti-miR miRNA Inhibitors. The fluorescent label enables direct observation of cellular uptake, distribution, and localization of the control. The molecule is labeled at its 5' end and has the same oligonucleotide sequence as unlabeled Anti-miR™ miRNA Inhibitor Negative Control #1 (SKU #AM17010).

Accessory Products:
Also available are Anti-miR™ miRNA Inhibitors (SKU #AM17000 and #AM17001). To select your miRNA sequence and order, please visit the GeneAssist™ miRNA Workflow Builder. Cy™3 dye-labeled Anti-miR™ Negative Controls (SKU #AM17011) are available. The Pre-miR™ miRNA Starter Kit (SKU #AM1540) can be used to optimize transfection of Pre-miR™ miRNA Precursors.

Stealth RNAi™ siRNA Negative Control, Med GC Invitrogen™

Stealth™ RNAi siRNA Negative Control, Med GC, is one of three negative controls for use with Stealth™ siRNA experiments. These controls are a great way to measure the effect of your experimental Stealth™ RNAi siRNA duplex versus background. The Stealth™ RNAi siRNA Negative Control Kit contains all three controls (Lo, Med, and Hi GC content—excluding sequences #2 and #3), and each is also available separately. These controls are:

• Designed with 3 levels of GC content (Lo, Med, Hi) with 3 sequences available per level, so researchers can match GC content to their experimental Stealth™ RNAi siRNA
• Not homologous to anything in the vertebrate transcriptome
• Tested to not induce a stress response

About Stealth™ RNAi siRNA Negative Controls
Stealth™ RNAi Negative Control Duplexes are ideal for use in RNA interference (RNAi) experiments as a control for sequence independent effects following Stealth™ RNAi delivery in any vertebrate cell line. Each Stealth™ RNAi Negative Control Duplex is designed to minimize sequence homology to any known vertebrate transcript. The Stealth™ RNAi Negative Control Duplexes differ from one another in their GC content, and are supplied in a ready-to-use format. 1X RNA Annealing/Dilution Buffer is included for dilution of the Stealth™ RNAi stock solution, if desired.

Stealth RNAi™ siRNA Negative Control Lo GC Duplex #3 Invitrogen™

Stealth RNAi™ siRNA Negative Controls are a great way to measure the effect of your experimental Stealth RNAi™ siRNA duplex versus background. These controls are:

• Designed in 3 levels of GC content (Lo, Med, Hi) with 3 sequences available per level so researchers can match GC content to their experimental Stealth RNAi™ siRNA
• Not homologous to anything in the vertebrate transcriptome
• Tested not to induce stress response Stealth RNAi™ siRNA

Negative Control Kit contains all three controls (Lo, Med, Hi GC content-excluding sequences #2 and #3) and each is also available separately. Get the ideal negative controls for your RNAi experiment so you can assess your results accurately.

Ambion™ In Vivo Negative Control #1 siRNA Invitrogen™

Ambion® In Vivo siRNA Controls
• Validated siRNA controls for optimizing siRNA experiments targeting coding and noncoding transcripts
• Positive Control siRNAs functionally tested in several common cell lines
• Negative Controls functionally proven to have minimal effects on cell proliferation and viability
• Include Silencer® Select modifications for enhanced specificity or Ambion® In Vivo modifications for increased stability
• For use in human, mouse, and rat cells

What are Ambion® In Vivo siRNAs?
Ambion® In Vivo siRNAs are designed using the proven Silencer® Select algorithm and incorporate chemical modifications for superior serum stability with in vivo delivery. The added serum stability does not compromise the performance of the siRNAs. Ambion® In Vivo siRNAs have been shown to be non-toxic in vivo (mouse) and non-immunogenic (cell-based assays). In cell-based assays, Ambion® In Vivo siRNAs exhibit equivalent or better potency than Silencer® Select siRNAs. The result is better confidence in your in vivo RNAi experiments, better knockdown when paired with an efficient delivery solution, and the assurance of limited non-specific effects.

Negative Control siRNAs
Negative control siRNAs—siRNAs with sequences that do not target any gene product—are essential for determining the effects of siRNA delivery and for providing a baseline to compare siRNA-treated samples. Ambion® In Vivo siRNAs and have no significant sequence similarity to mouse, rat, or human gene sequences. These negative control siRNAs have been tested by microarray analysis and shown to have minimal effects on gene expression. In addition, these negative control siRNA has been tested in multi-parametric cell-based assays and are proven to have no significant effect on cell proliferation, viability, or morphology in the cell lines tested.

Quality Control
Ambion® synthesizes and purifies each Ambion® In Vivo siRNA in state-of-the-art facilities to meet the highest quality standards. As part of our rigorous quality control procedures, each RNA oligonucleotide is analyzed by MALDI-TOF mass spectrometry, and analytical HPLC is used to monitor purity. To provide the utmost in quality, we also assess each annealed siRNA by gel electrophoresis to confirm that the strands anneal properly. The result is premium-quality siRNA that is purified and ready to use.

In Vivo Ready Controls
Our "In Vivo Ready" Control siRNAs are subjected to that extra level of purification and testing required for the introduction of siRNAs into animals. After HPLC purification and annealing, each siRNA is further purified utilizing a process that removes excess salt via a semi-permeable membrane. The result is highly pure siRNA with minimal salt content, suitable for in vivo applications. In vivo siRNAs are then sterile filtered, and tested for the presence of endotoxin. At concentrations of 50 µM in presence of deionized water, in vivo Ready siRNAs contain <5.0 mM Na+, <0.06 mM K+, and <0.02 mM Mg2+.

Silencer™ Cy™3-labeled Negative Control No. 1 siRNA Invitrogen™

Ambion® A fluorescently labeled negative control siRNA for the easy monitoring of siRNA uptake by fluorescence microscopy. Purified, annealed, and ready to use, the 5 nmol aliquot is supplied in a stable, dried format.

• Simplifies the monitoring of siRNA delivery
• Functionally tested in several common cell lines
• HPLC purified, duplexed, and ready to use

Visualize siRNA Uptake with Labeled siRNAs
Fluorescent labeled siRNAs allow for the direct observation siRNA cellular uptake, distribution, and localization. Fluorescently labeled siRNAs are commonly used to monitor transfection efficiency during optimization of transfection conditions. In general, siRNAs are rapidly taken up by and distributed throughout cells in as little as 4 hours. Fluorescent labeling of siRNAs allows for rapid monitoring of siRNA delivery by fluorescence microscopy, as opposed to unlabeled siRNAs, whose delivery must be monitored by target gene knockdown. The Silencer® Cy™3 Labeled Negative Control #1 siRNA sequence is identical to that of the Ambion Silencer® Negative Control# 1 siRNA (SKU #AM4611), but with the fluorescein derivative Cy™3 (excitation max. = 547, emission max. = 563 nm), on the 5' end of the sense strand, making it highly fluorescent when excited by the appropriate wavelength of light. Silencer® Cy™3 Labeled Negative Control #1 siRNA is suitable for monitoring siRNA uptake by chemical transfection or electroporation. Cy™3 labeling is recommended over FAM™ labeling when using electroporation because of its brighter fluorescence signal visible over a broader pH range. Electroporation produces a more diffuse siRNA distribution pattern, resulting in a less intense fluorescence signal than with chemical transfection.

Accessory Products:
Silencer® FAM™ Labeled Negative Control #1 siRNA (SKU #AM4620) is also available. Silencer® control siRNAs are unmodified 21-mers; they are designed to be used with Silencer® siRNAs, BLOCK-iT™ siRNAs, and other unmodified 21mer siRNAs. If you are using other siRNA products, such as Ambion® Silencer® Select siRNAs or STEALTH RNAi™ siRNAs, we recommend that you use control siRNAs designed for use with those siRNAs for more reliable experimental results.

mirVana™ miRNA Mimic, miR-1 Positive Control Invitrogen™

miRNA mimics are small, chemically modified double-stranded RNA molecules designed to specifically bind to and mimic endogenous miRNA molecules and enable miRNA functional analysis by up-regulation of miRNA activity.

We have now released the second generation of miRNA mimics, mirVana™ miRNA Mimics, which:
Exhibit maximum and consistent effect in vitro at low concentration
• Are non toxic
• Offer superior specificity due to unique Star strand modification
• Target the most updated Sanger content
• Can be used in vitro and in vivo, offering you consistency throughout your entire research project


Positive and negative controls are also available for use along with mirVana™ miRNA Mimics in miRNA up-regulation experiments.

mirVana™ miRNA Mimic miR-1 Positive Control
The mirVana™ miRNA Mimic miR-1 Positive Control is designed to mimic mature miR-1 miRNA after delivery to mammalian cells. miR-1 is associated with down-regulation of many genes. In particular, miR-1-mediated down-regulation of Protein Tyrosine Kinase 9 (PTK9) has been shown to occur at the mRNA level. miR-1 Positive Control is intended for use as a positive control for transfection experiments using mirVana™ miRNA Mimics. Monitor activity of miR-1 Positive Control through its gene silencing of PTK9, using real-time reverse transcription-PCR to detect PTK9 mRNA.

First Generation Mimic Controls
Our first-generation miRNA mimics (and controls), Ambion® Pre-miR™ miRNA Precursors, have lower specificity and potency, plus a lower strand specificity compared to the new mirVana™ miRNA Mimics. These first-generation mimics are recommended for completing ongoing in vitro miRNA studies. For new studies or in vivo studies, we highly recommend our new mirVana™ miRNA Mimics and their corresponding controls.

Silencer™ Select Negative Control No. 1 siRNA, in vivo ready Invitrogen™

Ambion® Silencer® Select Negative Control siRNA, In Vivo Ready is extensively validated and an ideal control for many aspects of an siRNA experiment. The control is highly purified as required for animal use. 250 nmol is provided.

• Premium-quality siRNA, highly purified and ready to use
• Functionally tested in several common cell lines
• Include Silencer® Select modifications for enhanced specificity
• For use in human, mouse, and rat cells

What are Silencer® Select siRNAs?
Silencer® Select siRNAs incorporate the latest improvements in siRNA design, off-target effect prediction algorithms, and chemical modifications to yield siRNAs with unrivalled efficacy, potency, and specificity. The result is fewer failed experiments due to poor silencing, and cleaner, more consistent phenotypic data.

Silencer® Select GAPDH Positive Control siRNA:
The Silencer® SelectGAPDH positive control is extensively validated and an ideal "test" siRNA for those just beginning siRNA experiments, because it is validated to work in multiple cell lines. Because it targets GAPDH mRNA (a common internal control), many assays are available to measure its effects, including real-time RT-PCR as well as the Ambion® KDalert™ GAPDH Assay Kit.

Quality Control:
Silencer® Select siRNAs are manufactured in a state-of-the-art facility under the highest quality standards. Rigorous quality control procedures include MALDI-TOF mass spectrometry analysis and analytical HPLC to monitor purity. Each siRNA is also assessed by gel electrophoresis to confirm that the strands anneal properly. Furthermore, In Vivo Ready siRNAs undergo additional purification with a semipermeable membrane to remove excess salt. This is followed by sterile filtratation and endotoxin testing. At concentrations of 50 µM in deionized water, In Vivo Ready siRNAs contain. <0.6 mM Na+, <2.0 mM K+, and <0.1 mM Mg2+. Silencer® Select siRNA controls have specific chemical modifications; they are designed to be used with Silencer® Select siRNAs. If you are using other siRNA products, such as BLOCK-iT™ siRNAs, Ambion® Silencer® siRNAs, or STEALTH RNAi™ siRNAs, we recommend that you use control siRNAs designed for use with those siRNAs for more reliable experimental results.

Stealth RNAi™ siRNA Negative Control Hi GC Invitrogen™

Stealth RNAi™ siRNA Negative Controls are a great way to measure the effect of your experimental Stealth RNAi™ siRNA duplex versus background. These controls are: –Designed in 3 levels of GC content (Lo, Med, Hi) with 3 sequences available per level so researchers can match GC content to their experimental Stealth RNAi™ siRNA –Not homologous to anything in vertebrate transcriptome –Tested not to induce stress response Stealth RNAi™ siRNA Negative Control Kit contains all three controls (Lo, Med, Hi GC content-excluding sequences #2 and #3) and each is also available separately. Get the ideal negative controls for your RNAi experiment so you can assess your results accurately.
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