Shop All Control Primers, Primer⁄Probe Sets & Templates

VetMAX™ Xeno™ Internal Positive Control - LIZ™ Assay Applied Biosystems™

The VetMAX™ Xeno™ Internal Positive Control (IPC) - LIZ™ Assay is a primer-probe mix that detects the Xeno internal positive control. The resultant Xeno data is used to determine the validity of diagnostic test results. The Xeno IPC LIZ assay is introduced during the qPCR preparation step and carried through the animal health PCR workflow.

Features of the VetMAX Xeno IPC - LIZ Assay include:
• Provides confidence that qPCR test results are accurate and actionable
• Easily integrates into any workflow
• Greatly reduces the likelihood of false negatives

The VetMAX Xeno IPC - LIZ Assay comes in a 25X concentration and easily integrates into animal health PCR workflows, regardless of the target assay, master mix, or sample preparation reagents already in place. Coupled with VetMAX™ Xeno™ Internal Positive Control RNA or VetMAX™ Xeno™ Internal Positive Control DNA, our proprietary design offers a verification layer to help ensure the qPCR test results are accurate and actionable by greatly reducing the likelihood of false negatives.

Xeno IPC assays are included in most VetMAX kits and have been successfully benchmarked against millions of genomes including those relevant to animal health. A VetMAX™ Xeno™ Internal Positive Control - VIC™ Assay (Cat. No. A29765) is also available.

Human ACTB (Beta Actin) Endogenous Control (VIC™/MGB probe, primer limited) Applied Biosystems™

The Applied Biosystems® Human ACTB (beta actin) Endogenous Control (VIC® ⁄ MGB Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when used with other gene expression assays. Probe is labeled with VIC™ dye - MGB and the primers are limited. Can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with Inventoried and Non-Inventoried TaqMan® Gene Expression Assays, Custom TaqMan® Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes.

Assay Details:

Gene Symbol: ACTB
RefSeq: NM_001101.2
Probe Exon Location:1
Amplicon Size: 171
Corresponding TaqMan Assay ID: Hs99999903_m1

TaqMan® Endogenous Controls

Eliminate months of assay design, formulation, and testing by using TaqMan® Endogenous Controls as your controls to quantify gene expression. This convenient collection of pre-designed probe and primer sets enables you to normalize the amount of sample RNA or DNA in a reaction.

Complete Solution for Quantitative Gene Expression

Having a hard time deciding what controls to use to quantify gene expression — even with detailed information on biological systems? Now, with TaqMan® Endogenous Controls, you can avoid all the trial-and-error of selecting controls for most common human, mouse, rat, and eukaryotic genes.

Simple to Use

All components of the TaqMan® Endogenous Controls are QC tested, formulated into a single 20X mix, and functionally tested. The controls are not only simple to use, but they are also fully compatible with universal conditions for two-step RT-PCR. Just add TaqMan® Universal PCR Master Mix (with or without AmpErase® UNG) and your cDNA sample to generate sensitive, reproducible, and truly quantitative gene expression data on Applied Biosystems instruments including the Applied Biosystems 7900HT, 7300, 7500 Real-Time PCR Systems, and the 7000 and 7700 Sequence Detection Systems.

Flexible Offering

We build each endogenous control using our proven 5' nuclease chemistry. For maximum flexibility, you can choose between two different reporter dyes and two quenchers:
• A FAM™ dye-labeled TaqMan® MGB probe (250nM, final concentration) and two unlabeled PCR primers (900nM each)
• A VIC® dye-labeled TaqMan® MGB probe (250nM, final concentration) and two unlabeled PCR primers (150nM each y primer limited)
• A VIC® dye-labeled TAMRA™ probe (250nM, final concentration) and two unlabeled PCR primers (150nM each y primer limited)

Choosing the Right Endogenous Control

Endogenous controls can normalize the expression levels of target genes by correcting differences in the amount of cDNA that is loaded into PCR reaction wells. For best results, verify that the endogenous control is consistently expressed in the sample set to be tested. Endogenous control expression must be uniform across all samples in the study. For multiplexing, ensure that the gene expression level of the endogenous control is greater than that of the target.

Multiplex vs. Singleplex PCR

All TaqMan® Endogenous Controls that contain probes labeled with the VIC® reporter dye are primer limited. This allows multiplexing of TaqMan® Endogenous Controls with target gene expression assays, provided that the control gene is more abundantly expressed than the target gene. All TaqMan® Endogenous Controls that contain probes labeled with the FAM™ reporter dye are not primer limited and are not intended for multiplexing .

For Research Use Only. Not for use in diagnostic procedures.

ERCC RNA Spike-In Mix Invitrogen™

Variation in RNA expression data can be attributed to a variety of factors including the quality of the starting material, the level of cellularity and RNA yield, the platform employed, and the person performing the experiment. To control for these sources of variability, a common set of external RNA controls has been developed by the External RNA Controls Consortium (ERCC), an ad-hoc group of academic, private, and public organizations hosted by the National Institute of Standards and Technology (NIST). The controls consist of a set of unlabeled, polyadenylated transcripts designed to be added to an RNA analysis experiment after sample isolation, in order to measure against defined performance criteria. Up until the design of such universally accepted controls, it has been difficult to execute a thorough investigation of fundamental analytical performance metrics. From the trusted brand of quality RNA reagents, Ambion® ERCC Spike-In Control Mixes are commercially available, pre-formulated blends of 92 transcripts, derived and traceable from NIST-certified DNA plasmids. The transcripts are designed to be 250 to 2,000 nt in length, which mimic natural eukaryotic mRNAs.

Key product features:

Achieve a standard measure for data comparison across gene expression experiments
• Measure sensitivity (lower limit of detection) and dynamic range of an experiment
• Quantitate differential gene expression

Unlock the Potential of RNA Analysis
RNA analysis, including gene expression profiling and whole transcriptome surveying, can lead to better understanding of expression patterns in disease states and provides greater insights into biological pathways and molecular mechanisms that regulate cell fate, development, and disease progression. Traditional methods of RNA analysis, such as qRT-PCR and microarrays, are well established but are being replaced by next-generation sequencing, a high-throughput digital alternative. Because each method carries multiple platforms, and with the need to compare various samples across platforms throughout the world, a standard measure for data comparison is necessary. As the capabilities of RNA analysis expand, the necessity to create a standardized view of data will become even more important.

Achieve and Compare Results with Confirmed Accuracy
Ambion® ERCC RNA Spike-In Controls are used to create a standard baseline measurement of RNA both within an experiment and across multiple experiments performed using various samples and platforms. With two spike-in mix formulations (Figure 1), various measurements, such as sensitivity or dynamic range, can be examined to assess different parameters in an experiment or across experiments (Figure 2). Furthermore, expression fold-change ratios between two samples can be calculated with a high degree of confidence using the highly concordant relationship between ExFold RNA Spike-In 1 and ExFold RNA Spike-In 2 (Figure 3). Measurements are determined via known molar concentrations for each transcript within a spike-in mix and through association of the two mixes using a combination of ratios across 4 different subgroups of the 92 transcripts. The controls are ideal for next generation sequencing experiments, such as on SOLiD™ System, and supported microarray platforms such as the Illumina® Sentrix® BeadChip.

Choose Among Flexible Options for ERCC Kit Configurations
Whether measuring dynamic range or gene expression fold-change, Ambion® ERCC Spike-In Control Mixes are available in two kit configurations to meet your experimental needs. Use the ERCC Spike-In Mix to determine the dynamic range and lower limit of detection on your platform, and use the ERCC ExFold Spike-In Mixes to assess the accuracy of differential gene expression measurements.
ERCC RNA Spike-In Mix 1*ExFold Spike-In Mix 1*ExFold Spike-In Mix 2*Nuclease-free Water
ERCC RNA Spike-In Mix (Cat. No. 4456740)10 µL1.75 mL
ERCC ExFold RNA Spike-In Mixes (Cat. No. 4456739)10 µL10 µL1.75 mL

* Although ERCC RNA Spike-In Mix 1 and ExFold Spike-In Mix 1 contain the same formulation of ERCC transcripts, do not substitute ERCC RNA Spike-In Mix 1 for ExFold Spike-In Mix 1 for fold-change assessment. Use only ExFold Spike-In Mix 1 and Mix 2 with the same manufacturing lot number.

For Research Use Only. Not for use in diagnostics procedures.

TaqMan™ Array Human Endogenous Control Applied Biosystems™

Applied Biosystems® TaqMan® Express Endogenous Control Plates use TaqMan® probe-based chemistry and are designed for use on the suite of Applied Biosystems® Real-Time PCR Systems – together the gold standard in quantitative gene expression offering the greatest sensitivity, specificity, reproducibility, and the broadest dynamic range.
The first aspect of any experiment looking at relative quantitation of gene expression should be the selection of endogenous control gene(s), to normalize for variations in sample input. The TaqMan® Express Human Endogenous Control Plate contains 32 genes, plated in triplicate, that have been shown to be good candidates for such an experiment. This collection of genes has been selected from literature searches and⁄or whole genome microarray tests carried out on numerous human tissues. They have been shown to be expressed constitutively and at moderate abundance across most test samples.

TaqMan™ Vaginal Microbiota Amplification Control Applied Biosystems™

The TaqMan® Vaginal Microbiota Amplification Control is designed to evaluate the amplification efficacy of the TaqMan® Vaginal Microbiota assays. It is a multi-target plasmid that includes amplicons for each of the qualified TaqMan Vaginal Microbiota assays, as well as control prokaryotic 16S rRNA and human RNase P RPPH1 gene sequences. This plasmid serves as a positive control for the TaqMan Vaginal Microbiota Assay collection. The microbial targets of the included amplicons are listed below.

The TaqMan Vaginal Microbiota Amplification Control can be included in vaginal microbiota profiling experiments to verify assay performance and to assist with troubleshooting.

Atopobium vaginae Prevotella bivia
Bacteroides fragilis Staphylococcus aureus
Bacterial vaginosis associated bacterium 2 (BVAB2) Streptococcus agalactiae
Chlamydia trachomatis Treponema pallidum
Enterococcus faecalis Candida albicans
Escherichia coli Candida dubliniensis
Gardnerella vaginalis Candida glabrata
Haemophilus ducreyi Candida krusei
Lactobacillus crispatus Candida lusitaniae
Lactobacillus gasseri Candida parapsilosis
Lactobacillus iners Candida tropicalis
Lactobacillus jensenii Mycoplasma genitalium
Megasphaera 1 Mycoplasma hominis
Megasphaera 2 Ureaplasma urealyticum
Mobiluncus curtisii Trichomonas vaginalis
Mobiluncus mulieris Herpes simplex 1
Neisseria gonorrhoeae Herpes simplex 2

Human PPIA (Cyclophilin A) Endogenous Control (VIC™/MGB probe, primer limited) Applied Biosystems™

The Applied Biosystems® Human PPIA (cyclophilin A) Endogenous Control (VIC® ⁄ MGB Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when used with other gene expression assays. Probe is labeled with VIC™ dye - MGB and the primers are limited. Can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with Inventoried and Non-Inventoried TaqMan® Gene Expression Assays, Custom TaqMan® Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes.

Assay Details:

Gene Symbol: PPIA
RefSeq: NM_021130.3
Probe Exon Location: 5
Amplicon Size: 98
Corresponding TaqMan Assay ID: Hs99999904_m1

TaqMan® Endogenous Controls

Eliminate months of assay design, formulation, and testing by using TaqMan® Endogenous Controls as your controls to quantify gene expression. This convenient collection of pre-designed probe and primer sets enables you to normalize the amount of sample RNA or DNA in a reaction.

Complete Solution for Quantitative Gene Expression

Having a hard time deciding what controls to use to quantify gene expression — even with detailed information on biological systems? Now, with TaqMan® Endogenous Controls, you can avoid all the trial-and-error of selecting controls for most common human, mouse, rat, and eukaryotic genes.

Simple to Use

All components of the TaqMan® Endogenous Controls are QC tested, formulated into a single 20X mix, and functionally tested. The controls are not only simple to use, but they are also fully compatible with universal conditions for two-step RT-PCR. Just add TaqMan® Universal PCR Master Mix (with or without AmpErase® UNG) and your cDNA sample to generate sensitive, reproducible, and truly quantitative gene expression data on Applied Biosystems instruments including the Applied Biosystems 7900HT, 7300, 7500 Real-Time PCR Systems, and the 7000 and 7700 Sequence Detection Systems.

Flexible Offering

We build each endogenous control using our proven 5' nuclease chemistry. For maximum flexibility, you can choose between two different reporter dyes and two quenchers:
• A FAM™ dye-labeled TaqMan® MGB probe (250nM, final concentration) and two unlabeled PCR primers (900nM each)
• A VIC® dye-labeled TaqMan® MGB probe (250nM, final concentration) and two unlabeled PCR primers (150nM each y primer limited)
• A VIC® dye-labeled TAMRA™ probe (250nM, final concentration) and two unlabeled PCR primers (150nM each y primer limited)

Choosing the Right Endogenous Control

Endogenous controls can normalize the expression levels of target genes by correcting differences in the amount of cDNA that is loaded into PCR reaction wells. For best results, verify that the endogenous control is consistently expressed in the sample set to be tested. Endogenous control expression must be uniform across all samples in the study. For multiplexing, ensure that the gene expression level of the endogenous control is greater than that of the target.

Multiplex vs. Singleplex PCR

All TaqMan® Endogenous Controls that contain probes labeled with the VIC® reporter dye are primer limited. This allows multiplexing of TaqMan® Endogenous Controls with target gene expression assays, provided that the control gene is more abundantly expressed than the target gene. All TaqMan® Endogenous Controls that contain probes labeled with the FAM™ reporter dye are not primer limited and are not intended for multiplexing .

For Research Use Only. Not for use in diagnostic procedures.

TaqMan™ Universal DNA Spike-In Control Applied Biosystems™

TaqMan Universal DNA Spike-In Control serves as an exogenous process control of a known concentration that is spiked into a sample at specific stages throughout the qPCR process. Measuring the concentration of the spiked-in sequence allows for its use as a serial quality control and it can be used to monitor recovery efficiency and to identify false negative results such as the presence of PCR inhibitors in molecular detection workflows. This is of particular importance when working with sample types that have a high level of inhibition. For a 5X version of this control, please see Cat. No. A39176.

RNA Control 250 Invitrogen™

The Ambion® RNA Control 250 provides a precise, accurate, and reproducible RNA concentration reference for use in conjunction with NanoDrop® Spectrophotometers. It is provided in two tubes containing 20 µL each.

• A stable, highly pure transcript at a precisely known concentration
• Used to verify the accuracy of NanoDrop® Spectrophotometer measurements
• Provided with THE RNA Storage Solution for blank measurements
• Enough for 20 measurements

RNA Control 250 consists of a single, ultra-pure, 2000 nt RNA transcript of a precisely defined sequence, molecular weight, and concentration. It is packaged in special non-stick tubes and includes the Ambion® THE RNA Storage Solution. The RNA Control 250 will maintain +/- 5% of stated concentration and >85% RNA integrity through 5 freeze/thaw cycles (10 duplicate measurements).

Human GAPD (GAPDH) Endogenous Control (VIC™/MGB probe, primer limited) Applied Biosystems™

The Applied Biosystems® Human GAPD (GAPDH) Endogenous Control (VIC® ⁄ MGB Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when used with other gene expression assays. Probe is labeled with VIC™ dye - MGB and the primers are limited. Can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with Inventoried and Non-Inventoried TaqMan® Gene Expression Assays, Custom TaqMan® Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes.

Assay Details:

Gene Symbol: GAPDH
RefSeq: NM_002046.3
Probe Exon Location:3
Amplicon Size: 122
Corresponding TaqMan Assay ID: Hs99999905_m1

TaqMan® Endogenous Controls

Eliminate months of assay design, formulation, and testing by using TaqMan® Endogenous Controls as your controls to quantify gene expression. This convenient collection of pre-designed probe and primer sets enables you to normalize the amount of sample RNA or DNA in a reaction.

Complete Solution for Quantitative Gene Expression

Having a hard time deciding what controls to use to quantify gene expression — even with detailed information on biological systems? Now, with TaqMan® Endogenous Controls, you can avoid all the trial-and-error of selecting controls for most common human, mouse, rat, and eukaryotic genes.

Simple to Use

All components of the TaqMan® Endogenous Controls are QC tested, formulated into a single 20X mix, and functionally tested. The controls are not only simple to use, but they are also fully compatible with universal conditions for two-step RT-PCR. Just add TaqMan® Universal PCR Master Mix (with or without AmpErase® UNG) and your cDNA sample to generate sensitive, reproducible, and truly quantitative gene expression data on Applied Biosystems instruments including the Applied Biosystems 7900HT, 7300, 7500 Real-Time PCR Systems, and the 7000 and 7700 Sequence Detection Systems.

Flexible Offering

We build each endogenous control using our proven 5' nuclease chemistry. For maximum flexibility, you can choose between two different reporter dyes and two quenchers:
• A FAM™ dye-labeled TaqMan® MGB probe (250nM, final concentration) and two unlabeled PCR primers (900nM each)
• A VIC® dye-labeled TaqMan® MGB probe (250nM, final concentration) and two unlabeled PCR primers (150nM each y primer limited)
• A VIC® dye-labeled TAMRA™ probe (250nM, final concentration) and two unlabeled PCR primers (150nM each y primer limited)

Choosing the Right Endogenous Control

Endogenous controls can normalize the expression levels of target genes by correcting differences in the amount of cDNA that is loaded into PCR reaction wells. For best results, verify that the endogenous control is consistently expressed in the sample set to be tested. Endogenous control expression must be uniform across all samples in the study. For multiplexing, ensure that the gene expression level of the endogenous control is greater than that of the target.

Multiplex vs. Singleplex PCR

All TaqMan® Endogenous Controls that contain probes labeled with the VIC® reporter dye are primer limited. This allows multiplexing of TaqMan® Endogenous Controls with target gene expression assays, provided that the control gene is more abundantly expressed than the target gene. All TaqMan® Endogenous Controls that contain probes labeled with the FAM™ reporter dye are not primer limited and are not intended for multiplexing .

For Research Use Only. Not for use in diagnostic procedures.

TaqMan™ Universal DNA Spike-In Control (5X) Applied Biosystems™

TaqMan Universal DNA Spike-In Control serves as an exogenous process control of a known concentration that is spiked into a sample at specific stages throughout the qPCR process. Measuring the concentration of the spiked-in sequence allows for its use as a serial quality control and it can be used to monitor recovery efficiency and to identify false negative results such as the presence of PCR inhibitors in molecular detection workflows. This is of particular importance when working with sample types that have a high level of inhibition. For a 1X version of this control, please see Cat. No. A39175.

Human RPLPO (Large Ribosomal Protein) Endogenous Control (FAM™/MGB probe, non-primer limited) Applied Biosystems™

The Applied Biosystems® Human RPLPO (large ribosomal protein) Endogenous Control (FAM™ ⁄ MGB Probe, Non-Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when used with other gene expression assays. Probe is labeled with 6FAM™ dye - MGB and the primers are not limited. Can be used for singleplex PCR reactions. Endogenous control is to be used with Inventoried and Non-Inventoried TaqMan® Gene Expression Assays, Custom TaqMan® Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes.

Assay Details:

Gene Symbol: RPLPO
RefSeq: NM_053275.3
Probe Exon Location:3
Amplicon Size: 105
Corresponding TaqMan Assay ID: Hs99999902_m1

TaqMan® Endogenous Controls

Eliminate months of assay design, formulation, and testing by using TaqMan® Endogenous Controls as your controls to quantify gene expression. This convenient collection of pre-designed probe and primer sets enables you to normalize the amount of sample RNA or DNA in a reaction.

Complete Solution for Quantitative Gene Expression

Having a hard time deciding what controls to use to quantify gene expression — even with detailed information on biological systems? Now, with TaqMan® Endogenous Controls, you can avoid all the trial-and-error of selecting controls for most common human, mouse, rat, and eukaryotic genes.

Simple to Use

All components of the TaqMan® Endogenous Controls are QC tested, formulated into a single 20X mix, and functionally tested. The controls are not only simple to use, but they are also fully compatible with universal conditions for two-step RT-PCR. Just add TaqMan® Universal PCR Master Mix (with or without AmpErase® UNG) and your cDNA sample to generate sensitive, reproducible, and truly quantitative gene expression data on Applied Biosystems instruments including the Applied Biosystems 7900HT, 7300, 7500 Real-Time PCR Systems, and the 7000 and 7700 Sequence Detection Systems.

Flexible Offering

We build each endogenous control using our proven 5' nuclease chemistry. For maximum flexibility, you can choose between two different reporter dyes and two quenchers:
• A FAM™ dye-labeled TaqMan® MGB probe (250nM, final concentration) and two unlabeled PCR primers (900nM each)
• A VIC® dye-labeled TaqMan® MGB probe (250nM, final concentration) and two unlabeled PCR primers (150nM each y primer limited)
• A VIC® dye-labeled TAMRA™ probe (250nM, final concentration) and two unlabeled PCR primers (150nM each y primer limited)

Choosing the Right Endogenous Control

Endogenous controls can normalize the expression levels of target genes by correcting differences in the amount of cDNA that is loaded into PCR reaction wells. For best results, verify that the endogenous control is consistently expressed in the sample set to be tested. Endogenous control expression must be uniform across all samples in the study. For multiplexing, ensure that the gene expression level of the endogenous control is greater than that of the target.

Multiplex vs. Singleplex PCR

All TaqMan® Endogenous Controls that contain probes labeled with the VIC® reporter dye are primer limited. This allows multiplexing of TaqMan® Endogenous Controls with target gene expression assays, provided that the control gene is more abundantly expressed than the target gene. All TaqMan® Endogenous Controls that contain probes labeled with the FAM™ reporter dye are not primer limited and are not intended for multiplexing .

For Research Use Only. Not for use in diagnostic procedures.

TaqMan™ RNase P Assay, ABY™ dye/QSY™ probe, primer-limited Applied Biosystems™

The Applied Biosystems® TaqMan® RNase P Assay with ABY® dye-labeled QSY® probe (primer limited) provides a pre-formulated assay for quantitating human RNase P. This assay enables relative gene expression quantification in cDNA samples when used with other gene expression assays. It consists of an ABY® dye-labeled QSY® probe plus sequence-specific forward and reverse primers. The assay can be used for multiplex or singleplex PCR reactions, and it is primer limited, so it can be used with samples that overexpress RNase P.

Benefits of this assay include:

• Eliminate assay design time using pre-designed primers and pre-designed ABY® dye-labeled TaqMan® probe
• Minimize experimental optimization time
• Use in conjunction with FAM™ or VIC® dye-labeled predesigned gene expression assays
• Formulated at a primer-limited concentration for use in multiplex reactions

Save time in assay design, formulation, and testing by using the TaqMan® RNase P Assay as your control when quantifying gene expression. This pre-designed probe and primer set enables you to normalize the amount of sample RNA or DNA in a reaction when used with a control sample. Or use it for relative gene expression quantification in cDNA samples when used with other gene expression assays.

In order to facilitate multiplex experiments, the assay formulation is primer limited, allowing the assay to be used with high RNase P expressors.

The ABY® dye is optimized for use with the 3rd filter of our QuantStudio™, ViiA™ 7, and 7500 series real-time PCR instruments.

VetMAX™ Xeno™ Internal Positive Control - VIC™ Assay Applied Biosystems™

The VetMAX™ Xeno™ Internal Positive Control (IPC) - VIC™ Assay is a primer-probe mix that detects the Xeno internal positive control. The resultant Xeno data is used to determine the validity of diagnostic test results. The Xeno IPC assay is introduced during the qPCR preparation step and carried through the animal health PCR workflow.

Features of the VetMAX Xeno IPC - VIC Assay include:
• Provides confidence that qPCR test results are accurate and actionable
• Easily integrates into any workflow
• Greatly reduces the likelihood of false negatives

The VetMAX Xeno IPC - VIC Assay comes in a 25X concentration and easily integrates into animal health PCR workflows, regardless of the target assay, master mix, or sample preparation reagents already in place. Coupled with VetMAX™ Xeno Internal Positive Control RNA or VetMAX™ Xeno Internal Positive Control DNA, our proprietary design offers a verification layer to help ensure the qPCR test results are accurate and actionable by greatly reducing the likelihood of false negatives.

Xeno IPC assays are included in most VetMAX kits and have been successfully benchmarked against millions of genomes including those relevant to animal health. A VetMAX™ Xeno™ Internal Positive Control - LIZ™ Assay (Cat. No. A29766) is also available.

TaqMan® Newcastle Disease Virus (NDV) and Xeno™ RNA Controls Applied Biosystems™

TaqMan® NDV and Xeno™ RNA Controls are synthetic positive controls for one-step, real-time, RT-PCR amplification of Newcastle Disease Virus RNA and Xeno™ RNA Internal Positive Control.

For Research Use Only. Not for use in diagnostics procedures.

Human ACTB (Beta Actin) Endogenous Control (VIC™/TAMRA™ probe, primer limited) Applied Biosystems™

The Applied Biosystems® Human ACTB (beta actin) Endogenous Control (VIC® ⁄ TAMRA Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when used with other TaqMan® gene expression assays. Probe is labeled with VIC™ dye - TAMRA™ dye and the primers are limited. Can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with Inventoried and Made to Order TaqMan® Gene Expression Assays, Custom TaqMan® Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes.

Assay Details:

Gene Symbol: ACTB
RefSeq: NM_001101.2
Probe Exon Location: 2-3
  

TaqMan® Endogenous Controls

Eliminate months of assay design, formulation, and testing by using TaqMan® Endogenous Controls as your controls to quantify gene expression. This convenient collection of pre-designed probe and primer sets enables you to normalize the amount of sample RNA or DNA in a reaction.

Complete Solution for Quantitative Gene Expression

Having a hard time deciding what controls to use to quantify gene expression — even with detailed information on biological systems? Now, with TaqMan® Endogenous Controls, you can avoid all the trial-and-error of selecting controls for most common human, mouse, rat, and eukaryotic genes.

Simple to Use

All components of the TaqMan® Endogenous Controls are QC tested, formulated into a single 20X mix, and functionally tested. The controls are not only simple to use, but they are also fully compatible with universal conditions for two-step RT-PCR. Just add TaqMan® Universal PCR Master Mix (with or without AmpErase® UNG) and your cDNA sample to generate sensitive, reproducible, and truly quantitative gene expression data on Applied Biosystems instruments including the Applied Biosystems 7900HT, 7300, 7500 Real-Time PCR Systems, and the 7000 and 7700 Sequence Detection Systems.

Flexible Offering

We build each endogenous control using our proven 5' nuclease chemistry. For maximum flexibility, you can choose between two different reporter dyes and two quenchers:
• A FAM™ dye-labeled TaqMan® MGB probe (250nM, final concentration) and two unlabeled PCR primers (900nM each)
• A VIC® dye-labeled TaqMan® MGB probe (250nM, final concentration) and two unlabeled PCR primers (150nM each y primer limited)
• A VIC® dye-labeled TAMRA™ probe (250nM, final concentration) and two unlabeled PCR primers (150nM each y primer limited)

Choosing the Right Endogenous Control

Endogenous controls can normalize the expression levels of target genes by correcting differences in the amount of cDNA that is loaded into PCR reaction wells. For best results, verify that the endogenous control is consistently expressed in the sample set to be tested. Endogenous control expression must be uniform across all samples in the study. For multiplexing, ensure that the gene expression level of the endogenous control is greater than that of the target.

Multiplex vs. Singleplex PCR

All TaqMan® Endogenous Controls that contain probes labeled with the VIC® reporter dye are primer limited. This allows multiplexing of TaqMan® Endogenous Controls with target gene expression assays, provided that the control gene is more abundantly expressed than the target gene. All TaqMan® Endogenous Controls that contain probes labeled with the FAM™ reporter dye are not primer limited and are not intended for multiplexing.

For Research Use Only. Not for use in diagnostic procedures.
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