Shop All Control Primers, Primer⁄Probe Sets & Templates

ERCC RNA Spike-In Mix (Invitrogen™)

Variation in RNA expression data can be attributed to a variety of factors including the quality of the starting material, the level of cellularity and RNA yield, the platform employed, and the person performing the experiment. To control for these sources of variability, a common set of external RNA controls has been developed by the External RNA Controls Consortium (ERCC), an ad-hoc group of academic, private, and public organizations hosted by the National Institute of Standards and Technology (NIST). The controls consist of a set of unlabeled, polyadenylated transcripts designed to be added to an RNA analysis experiment after sample isolation, in order to measure against defined performance criteria. Up until the design of such universally accepted controls, it has been difficult to execute a thorough investigation of fundamental analytical performance metrics. From the trusted brand of quality RNA reagents, Ambion® ERCC Spike-In Control Mixes are commercially available, pre-formulated blends of 92 transcripts, derived and traceable from NIST-certified DNA plasmids. The transcripts are designed to be 250 to 2,000 nt in length, which mimic natural eukaryotic mRNAs.

Key product features:

Achieve a standard measure for data comparison across gene expression experiments
• Measure sensitivity (lower limit of detection) and dynamic range of an experiment
• Quantitate differential gene expression

Unlock the Potential of RNA Analysis
RNA analysis, including gene expression profiling and whole transcriptome surveying, can lead to better understanding of expression patterns in disease states and provides greater insights into biological pathways and molecular mechanisms that regulate cell fate, development, and disease progression. Traditional methods of RNA analysis, such as qRT-PCR and microarrays, are well established but are being replaced by next-generation sequencing, a high-throughput digital alternative. Because each method carries multiple platforms, and with the need to compare various samples across platforms throughout the world, a standard measure for data comparison is necessary. As the capabilities of RNA analysis expand, the necessity to create a standardized view of data will become even more important.

Achieve and Compare Results with Confirmed Accuracy
Ambion® ERCC RNA Spike-In Controls are used to create a standard baseline measurement of RNA both within an experiment and across multiple experiments performed using various samples and platforms. With two spike-in mix formulations (Figure 1), various measurements, such as sensitivity or dynamic range, can be examined to assess different parameters in an experiment or across experiments (Figure 2). Furthermore, expression fold-change ratios between two samples can be calculated with a high degree of confidence using the highly concordant relationship between ExFold RNA Spike-In 1 and ExFold RNA Spike-In 2 (Figure 3). Measurements are determined via known molar concentrations for each transcript within a spike-in mix and through association of the two mixes using a combination of ratios across 4 different subgroups of the 92 transcripts. The controls are ideal for next generation sequencing experiments, such as on SOLiD™ System, and supported microarray platforms such as the Illumina® Sentrix® BeadChip.

Choose Among Flexible Options for ERCC Kit Configurations
Whether measuring dynamic range or gene expression fold-change, Ambion® ERCC Spike-In Control Mixes are available in two kit configurations to meet your experimental needs. Use the ERCC Spike-In Mix to determine the dynamic range and lower limit of detection on your platform, and use the ERCC ExFold Spike-In Mixes to assess the accuracy of differential gene expression measurements.
ERCC RNA Spike-In Mix 1*ExFold Spike-In Mix 1*ExFold Spike-In Mix 2*Nuclease-free Water
ERCC RNA Spike-In Mix (Cat. No. 4456740)10 µL1.75 mL
ERCC ExFold RNA Spike-In Mixes (Cat. No. 4456739)10 µL10 µL1.75 mL

* Although ERCC RNA Spike-In Mix 1 and ExFold Spike-In Mix 1 contain the same formulation of ERCC transcripts, do not substitute ERCC RNA Spike-In Mix 1 for ExFold Spike-In Mix 1 for fold-change assessment. Use only ExFold Spike-In Mix 1 and Mix 2 with the same manufacturing lot number.

For Research Use Only. Not for use in diagnostics procedures.

Human RPLPO (Large Ribosomal Protein) Endogenous Control (FAM™/MGB probe, non-primer limited) (Applied Biosystems™)

The Applied Biosystems® Human RPLPO (large ribosomal protein) Endogenous Control (FAM™ ⁄ MGB Probe, Non-Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when used with other gene expression assays. Probe is labeled with 6FAM™ dye - MGB and the primers are not limited. Can be used for singleplex PCR reactions. Endogenous control is to be used with Inventoried and Non-Inventoried TaqMan® Gene Expression Assays, Custom TaqMan® Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes.

Assay Details:

Gene Symbol: RPLPO
RefSeq: NM_053275.3
Probe Exon Location:3
Amplicon Size: 105
Corresponding TaqMan Assay ID: Hs99999902_m1

TaqMan® Endogenous Controls

Eliminate months of assay design, formulation, and testing by using TaqMan® Endogenous Controls as your controls to quantify gene expression. This convenient collection of pre-designed probe and primer sets enables you to normalize the amount of sample RNA or DNA in a reaction.

Complete Solution for Quantitative Gene Expression

Having a hard time deciding what controls to use to quantify gene expression — even with detailed information on biological systems? Now, with TaqMan® Endogenous Controls, you can avoid all the trial-and-error of selecting controls for most common human, mouse, rat, and eukaryotic genes.

Simple to Use

All components of the TaqMan® Endogenous Controls are QC tested, formulated into a single 20X mix, and functionally tested. The controls are not only simple to use, but they are also fully compatible with universal conditions for two-step RT-PCR. Just add TaqMan® Universal PCR Master Mix (with or without AmpErase® UNG) and your cDNA sample to generate sensitive, reproducible, and truly quantitative gene expression data on Applied Biosystems instruments including the Applied Biosystems 7900HT, 7300, 7500 Real-Time PCR Systems, and the 7000 and 7700 Sequence Detection Systems.

Flexible Offering

We build each endogenous control using our proven 5' nuclease chemistry. For maximum flexibility, you can choose between two different reporter dyes and two quenchers:
• A FAM™ dye-labeled TaqMan® MGB probe (250nM, final concentration) and two unlabeled PCR primers (900nM each)
• A VIC® dye-labeled TaqMan® MGB probe (250nM, final concentration) and two unlabeled PCR primers (150nM each y primer limited)
• A VIC® dye-labeled TAMRA™ probe (250nM, final concentration) and two unlabeled PCR primers (150nM each y primer limited)

Choosing the Right Endogenous Control

Endogenous controls can normalize the expression levels of target genes by correcting differences in the amount of cDNA that is loaded into PCR reaction wells. For best results, verify that the endogenous control is consistently expressed in the sample set to be tested. Endogenous control expression must be uniform across all samples in the study. For multiplexing, ensure that the gene expression level of the endogenous control is greater than that of the target.

Multiplex vs. Singleplex PCR

All TaqMan® Endogenous Controls that contain probes labeled with the VIC® reporter dye are primer limited. This allows multiplexing of TaqMan® Endogenous Controls with target gene expression assays, provided that the control gene is more abundantly expressed than the target gene. All TaqMan® Endogenous Controls that contain probes labeled with the FAM™ reporter dye are not primer limited and are not intended for multiplexing .

For Research Use Only. Not for use in diagnostic procedures.

VetMAX™ AIV Controls (Applied Biosystems™)

Note: The name of this product has changed. It was previously called TQMN AIV AND XENO RNA CONTROLS EACH.

TaqMan® AIV-M and Xeno™ RNA Controls are synthetic positive controls for one-step, real-time, RT-PCR amplification of Avian Influenza Virus matrix gene RNA and Xeno™ RNA Internal Positive Control.

For Research Use Only. Not for use in diagnostics procedures.

Mouse GAPD (GAPDH) Endogenous Control (FAM™/MGB probe, non-primer limited) (Applied Biosystems™)

The Applied Biosystems® Mouse GAPD (GAPDH) Endogenous Control (FAM™⁄MGB Probe, Non-Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when used with other gene expression assays. Probe is labeled with 6FAM™ dye - MGB and the primers are not limited. Can be used for singleplex PCR reactions. Endogenous control is to be used with Inventoried and Non-Inventoried TaqMan® Gene Expression Assays, Custom TaqMan® Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes.

Assay Details:

Gene Symbol: Gapdh
RefSeq: NM_008084.2
Probe Exon Location: 3
Amplicon Size: 107
Corresponding TaqMan Assay ID: Mm99999915_g1

TaqMan® Endogenous Controls

Eliminate months of assay design, formulation, and testing by using TaqMan® Endogenous Controls as your controls to quantify gene expression. This convenient collection of pre-designed probe and primer sets enables you to normalize the amount of sample RNA or DNA in a reaction.

Complete Solution for Quantitative Gene Expression

Having a hard time deciding what controls to use to quantify gene expression — even with detailed information on biological systems? Now, with TaqMan® Endogenous Controls, you can avoid all the trial-and-error of selecting controls for most common human, mouse, rat, and eukaryotic genes.

Simple to Use

All components of the TaqMan® Endogenous Controls are QC tested, formulated into a single 20X mix, and functionally tested. The controls are not only simple to use, but they are also fully compatible with universal conditions for two-step RT-PCR. Just add TaqMan® Universal PCR Master Mix (with or without AmpErase® UNG) and your cDNA sample to generate sensitive, reproducible, and truly quantitative gene expression data on Applied Biosystems instruments including the Applied Biosystems 7900HT, 7300, 7500 Real-Time PCR Systems, and the 7000 and 7700 Sequence Detection Systems.

Flexible Offering

We build each endogenous control using our proven 5' nuclease chemistry. For maximum flexibility, you can choose between two different reporter dyes and two quenchers:
• A FAM™ dye-labeled TaqMan® MGB probe (250nM, final concentration) and two unlabeled PCR primers (900nM each)
• A VIC® dye-labeled TaqMan® MGB probe (250nM, final concentration) and two unlabeled PCR primers (150nM each y primer limited)
• A VIC® dye-labeled TAMRA™ probe (250nM, final concentration) and two unlabeled PCR primers (150nM each y primer limited)

Choosing the Right Endogenous Control

Endogenous controls can normalize the expression levels of target genes by correcting differences in the amount of cDNA that is loaded into PCR reaction wells. For best results, verify that the endogenous control is consistently expressed in the sample set to be tested. Endogenous control expression must be uniform across all samples in the study. For multiplexing, ensure that the gene expression level of the endogenous control is greater than that of the target.

Multiplex vs. Singleplex PCR

All TaqMan® Endogenous Controls that contain probes labeled with the VIC® reporter dye are primer limited. This allows multiplexing of TaqMan® Endogenous Controls with target gene expression assays, provided that the control gene is more abundantly expressed than the target gene. All TaqMan® Endogenous Controls that contain probes labeled with the FAM™ reporter dye are not primer limited and are not intended for multiplexing .

For Research Use Only. Not for use in diagnostic procedures.

Human GUSB (Beta Glucuronidase) Endogenous Control (VIC™/TAMRA™ probe, primer limited) (Applied Biosystems™)

The Applied Biosystems® Human GUSB (beta glucuronidase) Endogenous Control (VIC® ⁄ TAMRA Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when used with other TaqMan® gene expression assays. Probe is labeled with VIC™ dye - TAMRA™ dye and the primers are limited. Can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with Inventoried and Made to Order TaqMan® Gene Expression Assays, Custom TaqMan® Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes.

Assay Details:

Gene Symbol: GUSB
RefSeq: NM_000181.2
Probe Exon Location: 11-12
Amplicon Size: 81

TaqMan® Endogenous Controls

Eliminate months of assay design, formulation, and testing by using TaqMan® Endogenous Controls as your controls to quantify gene expression. This convenient collection of pre-designed probe and primer sets enables you to normalize the amount of sample RNA or DNA in a reaction.

Complete Solution for Quantitative Gene Expression

Having a hard time deciding what controls to use to quantify gene expression — even with detailed information on biological systems? Now, with TaqMan® Endogenous Controls, you can avoid all the trial-and-error of selecting controls for most common human, mouse, rat, and eukaryotic genes.

Simple to Use

All components of the TaqMan® Endogenous Controls are QC tested, formulated into a single 20X mix, and functionally tested. The controls are not only simple to use, but they are also fully compatible with universal conditions for two-step RT-PCR. Just add TaqMan® Universal PCR Master Mix (with or without AmpErase® UNG) and your cDNA sample to generate sensitive, reproducible, and truly quantitative gene expression data on Applied Biosystems instruments including the Applied Biosystems 7900HT, 7300, 7500 Real-Time PCR Systems, and the 7000 and 7700 Sequence Detection Systems.

Flexible Offering

We build each endogenous control using our proven 5' nuclease chemistry. For maximum flexibility, you can choose between two different reporter dyes and two quenchers:
• A FAM™ dye-labeled TaqMan® MGB probe (250nM, final concentration) and two unlabeled PCR primers (900nM each)
• A VIC® dye-labeled TaqMan® MGB probe (250nM, final concentration) and two unlabeled PCR primers (150nM each y primer limited)
• A VIC® dye-labeled TAMRA™ probe (250nM, final concentration) and two unlabeled PCR primers (150nM each y primer limited)

Choosing the Right Endogenous Control

Endogenous controls can normalize the expression levels of target genes by correcting differences in the amount of cDNA that is loaded into PCR reaction wells. For best results, verify that the endogenous control is consistently expressed in the sample set to be tested. Endogenous control expression must be uniform across all samples in the study. For multiplexing, ensure that the gene expression level of the endogenous control is greater than that of the target.

Multiplex vs. Singleplex PCR

All TaqMan® Endogenous Controls that contain probes labeled with the VIC® reporter dye are primer limited. This allows multiplexing of TaqMan® Endogenous Controls with target gene expression assays, provided that the control gene is more abundantly expressed than the target gene. All TaqMan® Endogenous Controls that contain probes labeled with the FAM™ reporter dye are not primer limited and are not intended for multiplexing .

For Research Use Only. Not for use in diagnostic procedures.

Human ACTB (Beta Actin) Endogenous Control (FAM™/MGB probe, non-primer limited) (Applied Biosystems™)

The Applied Biosystems® Human ACTB (beta actin) Endogenous Control (FAM™ ⁄ MGB Probe, Non-Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when used with other gene expression assays. Probe is labeled with 6FAM™ dye - MGB and the primers are not limited. Can be used for singleplex PCR reactions. Endogenous control is to be used with Inventoried and Non-Inventoried TaqMan® Gene Expression Assays, Custom TaqMan® Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes.

Assay Details:

Gene Symbol: ACTB
RefSeq: NM_001101.2
Probe Exon Location:1
Amplicon Size: 171
Corresponding TaqMan Assay ID: Hs99999903_m1

TaqMan® Endogenous Controls

Eliminate months of assay design, formulation, and testing by using TaqMan® Endogenous Controls as your controls to quantify gene expression. This convenient collection of pre-designed probe and primer sets enables you to normalize the amount of sample RNA or DNA in a reaction.

Complete Solution for Quantitative Gene Expression

Having a hard time deciding what controls to use to quantify gene expression — even with detailed information on biological systems? Now, with TaqMan® Endogenous Controls, you can avoid all the trial-and-error of selecting controls for most common human, mouse, rat, and eukaryotic genes.

Simple to Use

All components of the TaqMan® Endogenous Controls are QC tested, formulated into a single 20X mix, and functionally tested. The controls are not only simple to use, but they are also fully compatible with universal conditions for two-step RT-PCR. Just add TaqMan® Universal PCR Master Mix (with or without AmpErase® UNG) and your cDNA sample to generate sensitive, reproducible, and truly quantitative gene expression data on Applied Biosystems instruments including the Applied Biosystems 7900HT, 7300, 7500 Real-Time PCR Systems, and the 7000 and 7700 Sequence Detection Systems.

Flexible Offering

We build each endogenous control using our proven 5' nuclease chemistry. For maximum flexibility, you can choose between two different reporter dyes and two quenchers:
• A FAM™ dye-labeled TaqMan® MGB probe (250nM, final concentration) and two unlabeled PCR primers (900nM each)
• A VIC® dye-labeled TaqMan® MGB probe (250nM, final concentration) and two unlabeled PCR primers (150nM each y primer limited)
• A VIC® dye-labeled TAMRA™ probe (250nM, final concentration) and two unlabeled PCR primers (150nM each y primer limited)

Choosing the Right Endogenous Control

Endogenous controls can normalize the expression levels of target genes by correcting differences in the amount of cDNA that is loaded into PCR reaction wells. For best results, verify that the endogenous control is consistently expressed in the sample set to be tested. Endogenous control expression must be uniform across all samples in the study. For multiplexing, ensure that the gene expression level of the endogenous control is greater than that of the target.

Multiplex vs. Singleplex PCR

All TaqMan® Endogenous Controls that contain probes labeled with the VIC® reporter dye are primer limited. This allows multiplexing of TaqMan® Endogenous Controls with target gene expression assays, provided that the control gene is more abundantly expressed than the target gene. All TaqMan® Endogenous Controls that contain probes labeled with the FAM™ reporter dye are not primer limited and are not intended for multiplexing .

For Research Use Only. Not for use in diagnostic procedures.

QuantumRNA™ Universal 18S Internal Standard (Invitrogen™)

The Ambion® QuantumRNA™ 18S Internal Standard with Competimer™ technology uses specially modified Competimer™s of the same sequence as the normal 18S primers that cannot be extended. By adjusting the ratio of 18S Competimer™s to normal 18S mRNA primers, the signal for 18S mRNA can be attenuated to the level of very rare messages. They are used for quantitative end-point RT-PCR and include sufficient reagents for 100 reactions. Because of its invariant expression across tissues and treatments, 18S ribosomal RNA is an ideal internal control for quantitative RNA analysis. The Universal 18S Internal Standards function across the broadest range of eukaryotes, including animals, plants, and many protozoa and produce a 315 bp PCR product.

Accessory Products:
The QuantumRNA™ Classic 18S Internal Standard (SKU# AM1716) produce a different 489 bp PCR product, and the QuantumRNA™ Classic II 18S Internal Standard (SKU# AM1717) produce a 324 bp PCR product.

Mouse ACTB (Actin, Beta) Endogenous Control (FAM™ Dye/MGB probe, Non-Primer Limited) (Applied Biosystems™)

The Applied Biosystems® Mouse ACTB (actin, beta) Endogenous Control (FAM™ Dye⁄MGB Probe, Non-Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when used with other TaqMan Gene Expression Assays. Probe is labeled with 6FAM™ dye - MGB and the primers are not limited. Can be used for singleplex PCR reactions. Endogenous control is to be used with Inventoried and Non-Inventoried TaqMan® Gene Expression Assays, Custom TaqMan® Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes.

Assay Details:

Gene Symbol: Actb
RefSeq: NM_007393.1
Probe Exon Location: 6
Amplicon Size: 115
Corresponding TaqMan Assay ID: Mm00607939_s1

TaqMan® Endogenous Controls

Eliminate months of assay design, formulation, and testing by using TaqMan® Endogenous Controls as your controls to quantify gene expression. This convenient collection of pre-designed probe and primer sets enables you to normalize the amount of sample RNA or DNA in a reaction.

Complete Solution for Quantitative Gene Expression

Having a hard time deciding what controls to use to quantify gene expression — even with detailed information on biological systems? Now, with TaqMan® Endogenous Controls, you can avoid all the trial-and-error of selecting controls for most common human, mouse, rat, and eukaryotic genes.

Simple to Use

All components of the TaqMan® Endogenous Controls are QC tested, formulated into a single 20X mix, and functionally tested. The controls are not only simple to use, but they are also fully compatible with universal conditions for two-step RT-PCR. Just add TaqMan® Universal PCR Master Mix (with or without AmpErase® UNG) and your cDNA sample to generate sensitive, reproducible, and truly quantitative gene expression data on Applied Biosystems instruments including the Applied Biosystems 7900HT, 7300, 7500 Real-Time PCR Systems, and the 7000 and 7700 Sequence Detection Systems.

Flexible Offering

We build each endogenous control using our proven 5' nuclease chemistry. For maximum flexibility, you can choose between two different reporter dyes and two quenchers:
• A FAM™ dye-labeled TaqMan® MGB probe (250nM, final concentration) and two unlabeled PCR primers (900nM each)
• A VIC® dye-labeled TaqMan® MGB probe (250nM, final concentration) and two unlabeled PCR primers (150nM each y primer limited)
• A VIC® dye-labeled TAMRA™ probe (250nM, final concentration) and two unlabeled PCR primers (150nM each y primer limited)

Choosing the Right Endogenous Control

Endogenous controls can normalize the expression levels of target genes by correcting differences in the amount of cDNA that is loaded into PCR reaction wells. For best results, verify that the endogenous control is consistently expressed in the sample set to be tested. Endogenous control expression must be uniform across all samples in the study. For multiplexing, ensure that the gene expression level of the endogenous control is greater than that of the target.

Multiplex vs. Singleplex PCR

All TaqMan® Endogenous Controls that contain probes labeled with the VIC® reporter dye are primer limited. This allows multiplexing of TaqMan® Endogenous Controls with target gene expression assays, provided that the control gene is more abundantly expressed than the target gene. All TaqMan® Endogenous Controls that contain probes labeled with the FAM™ reporter dye are not primer limited and are not intended for multiplexing .

For Research Use Only. Not for use in diagnostic procedures.

λ DNA (cIind 1 ts857 Sam 7) (Invitrogen™)

λ DNA (cIind 1 ts857 Sam 7) is isolated from a dam+ dcm+ E. coli LE597 lysogen. The DNA is suitable for use as a substrate for generating molecular size standards and for testing restriction endonuclease activity. Restriction endonucleases sensitive to dam or dcm methylation will not cleave this DNA.

Performance and Quality Testing: Purity, DNA structure, and selected restriction endonuclease fragmentation patterns are verified by agarose gel analysis.

tGFP mRNA for 1-Step Human IVT Kits (Thermo Scientific™)

tGFP mRNA (0.75 µg/µL in 0.1 mM EDTA) is intended as a control mRNA for use in in vitro protein expression kits, such as the Thermo Scientific Human In Vitro Protein Expression Kit.

More Product Data
Protocol for in vitro protein expression using mRNA templates
Glycoprotein expression in a human IVT system

TaqMan™ Array Human Endogenous Control Panel (Applied Biosystems™)

The Applied Biosystems® TaqMan® Endogenous Control Array is a 384-well micro fluidic card containing 16 human TaqMan® Gene Expression Assays commonly used as endogenous controls for for commonly used housekeeping genes and genes that exhibit minimal differential expression across different tissues.

TaqMan® Endogenous Control Array is pre-formatted and inventoried, cost-effective, convenient and easy to use without needing expensive robotics. Results are reproducible and consistent across samples, studies, and labs —the same data quality from card-to-card and lot-to-lot — even with different operators.

Human HPRT1 (HGPRT) Endogenous Control (VIC™/TAMRA™ probe, primer limited) (Applied Biosystems™)

The Applied Biosystems® Human HPRT1 (HGPRT) Endogenous Control (VIC® ⁄ TAMRA Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when used with other TaqMan® gene expression assays. Probe is labeled with VIC™ dye - TAMRA™ dye and the primers are limited. Can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with Inventoried and Made to Order TaqMan® Gene Expression Assays, Custom TaqMan® Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes.

Assay Details:

Gene Symbol: HPRT1
RefSeq: NM_000194.1
Probe Exon Location: 6-7
Amplicon Size: 100

TaqMan® Endogenous Controls

Eliminate months of assay design, formulation, and testing by using TaqMan® Endogenous Controls as your controls to quantify gene expression. This convenient collection of pre-designed probe and primer sets enables you to normalize the amount of sample RNA or DNA in a reaction.

Complete Solution for Quantitative Gene Expression

Having a hard time deciding what controls to use to quantify gene expression — even with detailed information on biological systems? Now, with TaqMan® Endogenous Controls, you can avoid all the trial-and-error of selecting controls for most common human, mouse, rat, and eukaryotic genes.

Simple to Use

All components of the TaqMan® Endogenous Controls are QC tested, formulated into a single 20X mix, and functionally tested. The controls are not only simple to use, but they are also fully compatible with universal conditions for two-step RT-PCR. Just add TaqMan® Universal PCR Master Mix (with or without AmpErase® UNG) and your cDNA sample to generate sensitive, reproducible, and truly quantitative gene expression data on Applied Biosystems instruments including the Applied Biosystems 7900HT, 7300, 7500 Real-Time PCR Systems, and the 7000 and 7700 Sequence Detection Systems.

Flexible Offering

We build each endogenous control using our proven 5' nuclease chemistry. For maximum flexibility, you can choose between two different reporter dyes and two quenchers:
• A FAM™ dye-labeled TaqMan® MGB probe (250nM, final concentration) and two unlabeled PCR primers (900nM each)
• A VIC® dye-labeled TaqMan® MGB probe (250nM, final concentration) and two unlabeled PCR primers (150nM each y primer limited)
• A VIC® dye-labeled TAMRA™ probe (250nM, final concentration) and two unlabeled PCR primers (150nM each y primer limited)

Choosing the Right Endogenous Control

Endogenous controls can normalize the expression levels of target genes by correcting differences in the amount of cDNA that is loaded into PCR reaction wells. For best results, verify that the endogenous control is consistently expressed in the sample set to be tested. Endogenous control expression must be uniform across all samples in the study. For multiplexing, ensure that the gene expression level of the endogenous control is greater than that of the target.

Multiplex vs. Singleplex PCR

All TaqMan® Endogenous Controls that contain probes labeled with the VIC® reporter dye are primer limited. This allows multiplexing of TaqMan® Endogenous Controls with target gene expression assays, provided that the control gene is more abundantly expressed than the target gene. All TaqMan® Endogenous Controls that contain probes labeled with the FAM™ reporter dye are not primer limited and are not intended for multiplexing .

For Research Use Only. Not for use in diagnostic procedures.

TaqMan™ Mutation Detection IPC Reagent Kit (Applied Biosystems™)

The TaqMan® Mutation Detection IPC Reagent Kit is a set of optional internal positive control reagents that can be duplexed with any TaqMan® Mutation Detection assay to provide a positive PCR control result. The IPC reagents can distinguish a mutation target negative result from a PCR failure result. The TaqMan® Mutation Detection IPC Reagent Kit is preoptimized for use with the TaqMan® Mutation Detection Assays. The IPC reagents can be duplexed with any TaqMan® Mutation Detection Assay and gDNA sample, without compromising the mutation detection assay reaction efficiency. This feature allows the mutation detection assays to detect low copies of mutated DNA in the presence of a robust PCR-positive signal from the IPC reagents.

Product Overview

The TaqMan® Mutation Detection IPC Reagent Kit includes:
50X Exogenous IPC template DNA (200 µL)
A synthetic template that contains the exogenous, non-human DNA target for the assay

10X Exogenous IPC Mix (1 mL)
A primer-limited TaqMan® assay designed to amplify an exogenous, non-human DNA target; the mix includes:
• Two PCR primers
• A TAMRA™ probe (VIC® dye-labeled)

Store at –15°C to –25° C, protected from light. Minimize freeze-thaw cycles.

Instrument Compatibility
TaqMan® Mutation Detection IPC Reagent Kit is compatible with the Applied Biosystems® ViiA™ 7, 7900HT, 7500, 7500 Fast, and StepOnePlus™ Real-Time PCR Systems.

Sample Type Compatibility
The kit can be used with FFPE samples, cell lines, and fresh frozen samples.

TaqMan™ Copy Number Reference Assay, mouse, Tert (Applied Biosystems™)

Mouse TaqMan® Copy Number Reference Assays are run with predesigned, Custom Plus, and Custom TaqMan® Copy Number Assays in a duplex, real-time PCR reaction to detect and measure copy number variations in the mouse genome. Mouse TaqMan® Copy Number Reference Assays are also compatible with the common vector marker and reporter gene predesigned TaqMan® Copy Number Assays. Two options for genes that can be used as endogenous reference genes in mice are offered: Tfrc and Tert. TaqMan® Copy Number Reference Assays are designed to unique genomic sequences in the mouse reference genome assembly (NCBI Build 37/mm9) and are required for relative quantitation of copy number targets.

TaqMan® Copy Number Assays quantitate the gene of interest and normalize to an endogenous reference gene known to be present in two copies in a diploid genome. Please note that TaqMan® Copy Number Reference Assays are species-specific.

Tert: The Alternative Reference Gene Option
TaqMan® Copy Number Reference Assays have a VIC® dye-labeled TAMRA™ probe and reference sequence-specific forward and reverse primers. The assays are not primer limited.

TaqMan® Copy Number Reference Assay, Mouse, Tfrc is recommended as the standard reference assay for copy number analysis in mice. This assay detects the transferrin receptor gene (Tfrc) on chromosome 16, cytoband 16qB3. The assay location is chr.16:32626732 on NCBI build 37. It has a 91 bp amplicon that maps within exon 17 of the Tfrc gene.

TaqMan® Copy Number Reference Assay, Mouse, Tert is an alternative reference assay; it is recommended in the event that the Tfrc assay functions poorly with a sample because of chromosomal aberrations or other issues. The assay targets the telomerase reverse transcriptase (Tert) gene on chromosome 13, cytoband 13qC1. The assay location is chr.13:73778992 on NCBI build 37. It has a 96 bp amplicon that maps within intron 8 of the Tert gene.

Simplest Workflow Available
TaqMan® Copy Number Assays have the simplest workflow of all currently available CNV analysis methods. The test assay (FAM™ dye labeled), the reference assay (VIC® dye labeled), your sample DNA, and TaqMan® Master Mix are combined and then run on an Applied Biosystems® Real-Time PCR System using the standard TaqMan® Copy Number Assay protocol. On average, set-up to primary analysis takes only 3-4 hours (including an approximately 2 hour PCR run).

Powerful Data Analysis Software
CopyCaller® Software was developed specifically for TaqMan® Copy Number Assay data analysis. This free, easy-to-use software utilizes a graphical interface that quickly calculates the possible copy numbers for a set of samples in a run. It also gives a confidence value for each copy number call, and has outlier functionality.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

Human PGK1 (Phosphoglycerate Kinase 1) Endogenous Control (FAM™/MGB Probe, non-primer limited) (Applied Biosystems™)

The Applied Biosystems® Human PGK1 (phosphoglycerate kinase 1) Endogenous Control (FAM™ ⁄ MGB Probe, Non-Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when used with other gene expression assays. Probe is labeled with 6FAM™ dye - MGB and the primers are not limited. Can be used for singleplex PCR reactions. Endogenous control is to be used with Inventoried and Non-Inventoried TaqMan® Gene Expression Assays, Custom TaqMan® Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes.

Assay Details:

Gene Symbol: PGK1
RefSeq: NM_000291.2
Probe Exon Location:4-5
Amplicon Size: 75
Corresponding TaqMan Assay ID: Hs9999906_m1

TaqMan® Endogenous Controls

Eliminate months of assay design, formulation, and testing by using TaqMan® Endogenous Controls as your controls to quantify gene expression. This convenient collection of pre-designed probe and primer sets enables you to normalize the amount of sample RNA or DNA in a reaction.

Complete Solution for Quantitative Gene Expression

Having a hard time deciding what controls to use to quantify gene expression — even with detailed information on biological systems? Now, with TaqMan® Endogenous Controls, you can avoid all the trial-and-error of selecting controls for most common human, mouse, rat, and eukaryotic genes.

Simple to Use

All components of the TaqMan® Endogenous Controls are QC tested, formulated into a single 20X mix, and functionally tested. The controls are not only simple to use, but they are also fully compatible with universal conditions for two-step RT-PCR. Just add TaqMan® Universal PCR Master Mix (with or without AmpErase® UNG) and your cDNA sample to generate sensitive, reproducible, and truly quantitative gene expression data on Applied Biosystems instruments including the Applied Biosystems 7900HT, 7300, 7500 Real-Time PCR Systems, and the 7000 and 7700 Sequence Detection Systems.

Flexible Offering

We build each endogenous control using our proven 5' nuclease chemistry. For maximum flexibility, you can choose between two different reporter dyes and two quenchers:
• A FAM™ dye-labeled TaqMan® MGB probe (250nM, final concentration) and two unlabeled PCR primers (900nM each)
• A VIC® dye-labeled TaqMan® MGB probe (250nM, final concentration) and two unlabeled PCR primers (150nM each y primer limited)
• A VIC® dye-labeled TAMRA™ probe (250nM, final concentration) and two unlabeled PCR primers (150nM each y primer limited)

Choosing the Right Endogenous Control

Endogenous controls can normalize the expression levels of target genes by correcting differences in the amount of cDNA that is loaded into PCR reaction wells. For best results, verify that the endogenous control is consistently expressed in the sample set to be tested. Endogenous control expression must be uniform across all samples in the study. For multiplexing, ensure that the gene expression level of the endogenous control is greater than that of the target.

Multiplex vs. Singleplex PCR

All TaqMan® Endogenous Controls that contain probes labeled with the VIC® reporter dye are primer limited. This allows multiplexing of TaqMan® Endogenous Controls with target gene expression assays, provided that the control gene is more abundantly expressed than the target gene. All TaqMan® Endogenous Controls that contain probes labeled with the FAM™ reporter dye are not primer limited and are not intended for multiplexing .

For Research Use Only. Not for use in diagnostic procedures.