Shop All Control Primers, Primer⁄Probe Sets & Templates

VetMAX™ Xeno™ Internal Positive Control - LIZ™ Assay (Applied Biosystems™)

The VetMAX™ Xeno™ Internal Positive Control (IPC) - LIZ™ Assay is a primer-probe mix that detects the Xeno internal positive control. The resultant Xeno data is used to determine the validity of diagnostic test results. The Xeno IPC LIZ assay is introduced during the qPCR preparation step and carried through the animal health PCR workflow.

Features of the VetMAX Xeno IPC - LIZ Assay include:
• Provides confidence that qPCR test results are accurate and actionable
• Easily integrates into any workflow
• Greatly reduces the likelihood of false negatives

The VetMAX Xeno IPC - LIZ Assay comes in a 25X concentration and easily integrates into animal health PCR workflows, regardless of the target assay, master mix, or sample preparation reagents already in place. Coupled with VetMAX™ Xeno™ Internal Positive Control RNA or VetMAX™ Xeno™ Internal Positive Control DNA, our proprietary design offers a verification layer to help ensure the qPCR test results are accurate and actionable by greatly reducing the likelihood of false negatives.

Xeno IPC assays are included in most VetMAX kits and have been successfully benchmarked against millions of genomes including those relevant to animal health. A VetMAX™ Xeno™ Internal Positive Control - VIC™ Assay (Cat. No. A29767) is also available.

Human TBP (TATA-box binding Protein) Endogenous Control (VIC™/MGB probe, primer limited) (Applied Biosystems™)

The Applied Biosystems® Human TBP (TATA-box binding protein) Endogenous Control (VIC® ⁄ MGB Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when used with other gene expression assays. Probe is labeled with VIC™ dye - MGB and the primers are limited. Can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with Inventoried and Non-Inventoried TaqMan® Gene Expression Assays, Custom TaqMan® Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes.

Assay Details:

Gene Symbol: TBP
RefSeq: M55654.1
Probe Exon Location:6
Amplicon Size: 127
Corresponding TaqMan Assay ID: Hs99999910_m1

TaqMan® Endogenous Controls

Eliminate months of assay design, formulation, and testing by using TaqMan® Endogenous Controls as your controls to quantify gene expression. This convenient collection of pre-designed probe and primer sets enables you to normalize the amount of sample RNA or DNA in a reaction.

Complete Solution for Quantitative Gene Expression

Having a hard time deciding what controls to use to quantify gene expression — even with detailed information on biological systems? Now, with TaqMan® Endogenous Controls, you can avoid all the trial-and-error of selecting controls for most common human, mouse, rat, and eukaryotic genes.

Simple to Use

All components of the TaqMan® Endogenous Controls are QC tested, formulated into a single 20X mix, and functionally tested. The controls are not only simple to use, but they are also fully compatible with universal conditions for two-step RT-PCR. Just add TaqMan® Universal PCR Master Mix (with or without AmpErase® UNG) and your cDNA sample to generate sensitive, reproducible, and truly quantitative gene expression data on Applied Biosystems instruments including the Applied Biosystems 7900HT, 7300, 7500 Real-Time PCR Systems, and the 7000 and 7700 Sequence Detection Systems.

Flexible Offering

We build each endogenous control using our proven 5' nuclease chemistry. For maximum flexibility, you can choose between two different reporter dyes and two quenchers:
• A FAM™ dye-labeled TaqMan® MGB probe (250nM, final concentration) and two unlabeled PCR primers (900nM each)
• A VIC® dye-labeled TaqMan® MGB probe (250nM, final concentration) and two unlabeled PCR primers (150nM each y primer limited)
• A VIC® dye-labeled TAMRA™ probe (250nM, final concentration) and two unlabeled PCR primers (150nM each y primer limited)

Choosing the Right Endogenous Control

Endogenous controls can normalize the expression levels of target genes by correcting differences in the amount of cDNA that is loaded into PCR reaction wells. For best results, verify that the endogenous control is consistently expressed in the sample set to be tested. Endogenous control expression must be uniform across all samples in the study. For multiplexing, ensure that the gene expression level of the endogenous control is greater than that of the target.

Multiplex vs. Singleplex PCR

All TaqMan® Endogenous Controls that contain probes labeled with the VIC® reporter dye are primer limited. This allows multiplexing of TaqMan® Endogenous Controls with target gene expression assays, provided that the control gene is more abundantly expressed than the target gene. All TaqMan® Endogenous Controls that contain probes labeled with the FAM™ reporter dye are not primer limited and are not intended for multiplexing .

For Research Use Only. Not for use in diagnostic procedures.

Human TFRC (CD71) (Transferrin Receptor) Endogenous Control (VIC™/MGB probe, primer limited) (Applied Biosystems™)

The Applied Biosystems® Human TFRC (CD71) (transferrin receptor) Endogenous Control (VIC® ⁄ MGB Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when used with other gene expression assays. Probe is labeled with VIC™ dye - MGB and the primers are limited. Can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with Inventoried and Non-Inventoried TaqMan® Gene Expression Assays, Custom TaqMan® Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes.

Assay Details:

Gene Symbol: TFRC
RefSeq: NM_003234.1
Probe Exon Location:14
Corresponding TaqMan Assay ID: Hs99999911_m1

TaqMan® Endogenous Controls

Eliminate months of assay design, formulation, and testing by using TaqMan® Endogenous Controls as your controls to quantify gene expression. This convenient collection of pre-designed probe and primer sets enables you to normalize the amount of sample RNA or DNA in a reaction.

Complete Solution for Quantitative Gene Expression

Having a hard time deciding what controls to use to quantify gene expression — even with detailed information on biological systems? Now, with TaqMan® Endogenous Controls, you can avoid all the trial-and-error of selecting controls for most common human, mouse, rat, and eukaryotic genes.

Simple to Use

All components of the TaqMan® Endogenous Controls are QC tested, formulated into a single 20X mix, and functionally tested. The controls are not only simple to use, but they are also fully compatible with universal conditions for two-step RT-PCR. Just add TaqMan® Universal PCR Master Mix (with or without AmpErase® UNG) and your cDNA sample to generate sensitive, reproducible, and truly quantitative gene expression data on Applied Biosystems instruments including the Applied Biosystems 7900HT, 7300, 7500 Real-Time PCR Systems, and the 7000 and 7700 Sequence Detection Systems.

Flexible Offering

We build each endogenous control using our proven 5' nuclease chemistry. For maximum flexibility, you can choose between two different reporter dyes and two quenchers:
• A FAM™ dye-labeled TaqMan® MGB probe (250nM, final concentration) and two unlabeled PCR primers (900nM each)
• A VIC® dye-labeled TaqMan® MGB probe (250nM, final concentration) and two unlabeled PCR primers (150nM each y primer limited)
• A VIC® dye-labeled TAMRA™ probe (250nM, final concentration) and two unlabeled PCR primers (150nM each y primer limited)

Choosing the Right Endogenous Control

Endogenous controls can normalize the expression levels of target genes by correcting differences in the amount of cDNA that is loaded into PCR reaction wells. For best results, verify that the endogenous control is consistently expressed in the sample set to be tested. Endogenous control expression must be uniform across all samples in the study. For multiplexing, ensure that the gene expression level of the endogenous control is greater than that of the target.

Multiplex vs. Singleplex PCR

All TaqMan® Endogenous Controls that contain probes labeled with the VIC® reporter dye are primer limited. This allows multiplexing of TaqMan® Endogenous Controls with target gene expression assays, provided that the control gene is more abundantly expressed than the target gene. All TaqMan® Endogenous Controls that contain probes labeled with the FAM™ reporter dye are not primer limited and are not intended for multiplexing .

For Research Use Only. Not for use in diagnostic procedures.

TaqMan™ Array Human Endogenous Control (Applied Biosystems™)

Applied Biosystems® TaqMan® Express Endogenous Control Plates use TaqMan® probe-based chemistry and are designed for use on the suite of Applied Biosystems® Real-Time PCR Systems – together the gold standard in quantitative gene expression offering the greatest sensitivity, specificity, reproducibility, and the broadest dynamic range.
The first aspect of any experiment looking at relative quantitation of gene expression should be the selection of endogenous control gene(s), to normalize for variations in sample input. The TaqMan® Express Human Endogenous Control Plate contains 32 genes, plated in triplicate, that have been shown to be good candidates for such an experiment. This collection of genes has been selected from literature searches and⁄or whole genome microarray tests carried out on numerous human tissues. They have been shown to be expressed constitutively and at moderate abundance across most test samples.

Human ACTB (Beta Actin) Endogenous Control (FAM™/MGB probe, non-primer limited) (Applied Biosystems™)

The Applied Biosystems® Human ACTB (actin, beta) Endogenous Control (FAM™ Dye ⁄ MGB Probe, Non-Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when used with other TaqMan® Gene Expression Assays. Probe is labeled with 6FAM™ dye - MGB and the primers are not limited. Can be used for singleplex PCR reactions. Endogenous control is to be used with Inventoried and Non-Inventoried TaqMan® Gene Expression Assays, Custom TaqMan® Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes.

Assay Details:

Gene Symbol: ACTB
RefSeq: NM_001101.2
Probe Exon Location:1
Amplicon Size: 171
Corresponding TaqMan Assay ID: Hs99999903_m1

TaqMan® Endogenous Controls

Eliminate months of assay design, formulation, and testing by using TaqMan® Endogenous Controls as your controls to quantify gene expression. This convenient collection of pre-designed probe and primer sets enables you to normalize the amount of sample RNA or DNA in a reaction.

Complete Solution for Quantitative Gene Expression

Having a hard time deciding what controls to use to quantify gene expression — even with detailed information on biological systems? Now, with TaqMan® Endogenous Controls, you can avoid all the trial-and-error of selecting controls for most common human, mouse, rat, and eukaryotic genes.

Simple to Use

All components of the TaqMan® Endogenous Controls are QC tested, formulated into a single 20X mix, and functionally tested. The controls are not only simple to use, but they are also fully compatible with universal conditions for two-step RT-PCR. Just add TaqMan® Universal PCR Master Mix (with or without AmpErase® UNG) and your cDNA sample to generate sensitive, reproducible, and truly quantitative gene expression data on Applied Biosystems instruments including the Applied Biosystems 7900HT, 7300, 7500 Real-Time PCR Systems, and the 7000 and 7700 Sequence Detection Systems.

Flexible Offering

We build each endogenous control using our proven 5' nuclease chemistry. For maximum flexibility, you can choose between two different reporter dyes and two quenchers:
• A FAM™ dye-labeled TaqMan® MGB probe (250nM, final concentration) and two unlabeled PCR primers (900nM each)
• A VIC® dye-labeled TaqMan® MGB probe (250nM, final concentration) and two unlabeled PCR primers (150nM each y primer limited)
• A VIC® dye-labeled TAMRA™ probe (250nM, final concentration) and two unlabeled PCR primers (150nM each y primer limited)

Choosing the Right Endogenous Control

Endogenous controls can normalize the expression levels of target genes by correcting differences in the amount of cDNA that is loaded into PCR reaction wells. For best results, verify that the endogenous control is consistently expressed in the sample set to be tested. Endogenous control expression must be uniform across all samples in the study. For multiplexing, ensure that the gene expression level of the endogenous control is greater than that of the target.

Multiplex vs. Singleplex PCR

All TaqMan® Endogenous Controls that contain probes labeled with the VIC® reporter dye are primer limited. This allows multiplexing of TaqMan® Endogenous Controls with target gene expression assays, provided that the control gene is more abundantly expressed than the target gene. All TaqMan® Endogenous Controls that contain probes labeled with the FAM™ reporter dye are not primer limited and are not intended for multiplexing .

For Research Use Only. Not for use in diagnostic procedures.

Human ACTB (Beta Actin) Endogenous Control (VIC™/TAMRA™ probe, primer limited) (Applied Biosystems™)

The Applied Biosystems® Human ACTB (beta actin) Endogenous Control (VIC® ⁄ TAMRA Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when used with other TaqMan® gene expression assays. Probe is labeled with VIC™ dye - TAMRA™ dye and the primers are limited. Can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with Inventoried and Made to Order TaqMan® Gene Expression Assays, Custom TaqMan® Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes.

Assay Details:

Gene Symbol: ACTB
RefSeq: NM_001101.2
Probe Exon Location: 2-3
  

TaqMan® Endogenous Controls

Eliminate months of assay design, formulation, and testing by using TaqMan® Endogenous Controls as your controls to quantify gene expression. This convenient collection of pre-designed probe and primer sets enables you to normalize the amount of sample RNA or DNA in a reaction.

Complete Solution for Quantitative Gene Expression

Having a hard time deciding what controls to use to quantify gene expression — even with detailed information on biological systems? Now, with TaqMan® Endogenous Controls, you can avoid all the trial-and-error of selecting controls for most common human, mouse, rat, and eukaryotic genes.

Simple to Use

All components of the TaqMan® Endogenous Controls are QC tested, formulated into a single 20X mix, and functionally tested. The controls are not only simple to use, but they are also fully compatible with universal conditions for two-step RT-PCR. Just add TaqMan® Universal PCR Master Mix (with or without AmpErase® UNG) and your cDNA sample to generate sensitive, reproducible, and truly quantitative gene expression data on Applied Biosystems instruments including the Applied Biosystems 7900HT, 7300, 7500 Real-Time PCR Systems, and the 7000 and 7700 Sequence Detection Systems.

Flexible Offering

We build each endogenous control using our proven 5' nuclease chemistry. For maximum flexibility, you can choose between two different reporter dyes and two quenchers:
• A FAM™ dye-labeled TaqMan® MGB probe (250nM, final concentration) and two unlabeled PCR primers (900nM each)
• A VIC® dye-labeled TaqMan® MGB probe (250nM, final concentration) and two unlabeled PCR primers (150nM each y primer limited)
• A VIC® dye-labeled TAMRA™ probe (250nM, final concentration) and two unlabeled PCR primers (150nM each y primer limited)

Choosing the Right Endogenous Control

Endogenous controls can normalize the expression levels of target genes by correcting differences in the amount of cDNA that is loaded into PCR reaction wells. For best results, verify that the endogenous control is consistently expressed in the sample set to be tested. Endogenous control expression must be uniform across all samples in the study. For multiplexing, ensure that the gene expression level of the endogenous control is greater than that of the target.

Multiplex vs. Singleplex PCR

All TaqMan® Endogenous Controls that contain probes labeled with the VIC® reporter dye are primer limited. This allows multiplexing of TaqMan® Endogenous Controls with target gene expression assays, provided that the control gene is more abundantly expressed than the target gene. All TaqMan® Endogenous Controls that contain probes labeled with the FAM™ reporter dye are not primer limited and are not intended for multiplexing.

For Research Use Only. Not for use in diagnostic procedures.

VetMAX™ Xeno™ Internal Positive Control RNA (Applied Biosystems™)

VetMAX™ Xeno™ Internal Positive Control (IPC) RNA serves as an internal positive control for the RNA purification process and helps monitor for the presence of PCR inhibitors in animal health molecular detection workflows. This is of particular importance when working with sample types that have a high level of inhibitors such as oral fluids. Xeno IPC RNA is introduced at the nucleic acid isolation/preparation step and carried through the animal health PCR workflow.

Features of VetMAX Xeno IPC RNA include:
• Provides confidence that PCR test results are accurate and actionable
• Easily integrates into any workflow
• Greatly reduces the likelihood of false negatives

VetMAX Xeno IPC RNA comes in a concentration of 10,000 copies/µL and easily integrates into animal health PCR workflows, regardless of the target assay, master mix, or sample preparation reagents already in place. Coupled with a Xeno internal positive control assay, our proprietary design offers a verification layer to help ensure the qPCR test results are accurate and actionable by greatly reducing the likelihood of false negatives.

Xeno IPC RNA is a component of our licensed VetMAX Gold detection kits, is recommended in the American Association of Veterinary Diagnosticians guidelines, and has been successfully benchmarked against millions of genomes including those relevant to animal health. Xeno IPC DNA is also available (Cat. No. A29762).

TaqMan™ Copy Number Reference Assay, human, RNase P (Applied Biosystems™)

Human TaqMan® Copy Number Reference Assays are run with human TaqMan® Copy Number Assays in a duplex real-time PCR reaction to detect and measure copy number variations (CNVs) and smaller regions in the human genome. Two options for genes that can be used as endogenous reference genes in humans are offered: RNase P and TERT. TaqMan® Copy Number Reference Assays are designed to unique genomic sequences in the reference genome assembly (build GRCh38) and are required for relative quantitation of copy number targets.

TaqMan® Copy Number Assays quantitate the gene of interest and normalize to an endogenous reference gene known to be present in two copies in a diploid genome. Please note that TaqMan® Copy Number Reference Assays are species specific.

RNase P: The Standard Reference Gene Option
TaqMan® Copy Number Reference Assays have a VIC® dye–labeled TAMRA™ probe and reference sequence–specific forward and reverse primers. The assays are not primer-limited.

TaqMan® Copy Number Reference Assay RNase P is recommended as the standard reference assay for copy number analysis. This assay detects the Ribonuclease P RNA component H1 (H1RNA) gene (RPPH1) on chromosome 14, cytoband 14q11.2. The assay location is chr.14:20811565 on build GRCh38. It has an 87 bp amplicon that maps within the single exon RPPH1 gene.

TaqMan® Copy Number Reference Assay TERT is an alternative reference assay; it is recommended in the event that the RNase P assay functions poorly with a sample because of chromosomal aberrations or other issues. This assay targets the telomerase reverse transcriptase (TERT) gene located on chromosome 5, cytoband 5p15.33. The assay location is chr5:1253257 on build GRCh38. It has an 88 bp amplicon that maps within exon 16 of the TERT gene.

Simplest Copy Number Analysis Workflow
TaqMan® Copy Number Assays have the simplest workflow of all currently available copy number analysis methods. The test assay (FAM™ dye–labeled), the reference assay (VIC® dye–labeled), your sample DNA, and TaqMan® Master Mix are combined and then run on an Applied Biosystems® real-time PCR system using the standard TaqMan® Copy Number Assay protocol. On average, setup to primary analysis takes only 3–4 hr (including an approximately 2 hour PCR run).

Powerful Data analysis Software
CopyCaller® Software was developed specifically for TaqMan® Copy Number Assay data analysis. This free, easy-to-use software utilizes a graphical interface that quickly calculates the possible copy numbers for a set of samples in a run. It also gives a confidence value for each copy number call, and has outlier functionality.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

Total RNA Control (Human) (Applied Biosystems™)

Human Total RNA is provided at a convenient reverse-transcription–ready concentration for all RT-PCR reactions. TaqMan® Control Total RNA is from Raji cells (Burkitt's lymphoma), and is conveniently packaged in a PCR-ready concentration for use as a control template in RT-PCR reactions. The Human total RNA is provided at a concentration of 50 ng/µl.

QuantumRNA™ Classic 18S Internal Standard (Invitrogen™)

The Ambion® QuantumRNA™ 18S Internal Standard with Competimer™ technology uses specially modified Competimer™s of the same sequence as the normal 18S primers that cannot be extended. By adjusting the ratio of 18S Competimer™s to normal 18S mRNA primers, the signal for 18S mRNA can be attenuated to the level of very rare messages. They are used for quantitative end-point RT-PCR and include sufficient reagents for 100 reactions. Because of its invariant expression across tissues and treatments, 18S ribosomal RNA is an ideal internal control for quantitative RNA analysis. The Classic 18S Internal Standards contain the same primers/Competimer™s that were part of the original QuantumRNA™ 18S Internal Standards Module. They produce a 489 bp PCR product.

Accessory Products:
The QuantumRNA™ Classic II 18S Internal Standard (SKU# AM1717) produce a 324 bp PCR product, and the QuantumRNA™ Universal 18S Internal Standard (SKU# AM1718) function across the broadest range of eukaryotes, including animals, plants, and many protozoa and produce a 315 bp PCR product.

Eukaryotic 18S rRNA Endogenous Control (VIC™/TAMRA™ probe, primer limited) (Applied Biosystems™)

The Applied Biosystems® Eukaryotic 18S rRNA Endogenous Control (VIC® ⁄ TAMRA Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when used with other TaqMan® gene expression assays. Probe is labeled with VIC™ dye - TAMRA™ dye and the primers are limited. Can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with Inventoried and Made to Order TaqMan® Gene Expression Assays, Custom TaqMan® Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes.

Assay Details:

Gene Symbol: 18S
RefSeq: X03205.1
Probe Exon Location: NA
Amplicon Size: 187

TaqMan® Endogenous Controls

Eliminate months of assay design, formulation, and testing by using TaqMan® Endogenous Controls as your controls to quantify gene expression. This convenient collection of pre-designed probe and primer sets enables you to normalize the amount of sample RNA or DNA in a reaction.

Complete Solution for Quantitative Gene Expression

Having a hard time deciding what controls to use to quantify gene expression — even with detailed information on biological systems? Now, with TaqMan® Endogenous Controls, you can avoid all the trial-and-error of selecting controls for most common human, mouse, rat, and eukaryotic genes.

Simple to Use

All components of the TaqMan® Endogenous Controls are QC tested, formulated into a single 20X mix, and functionally tested. The controls are not only simple to use, but they are also fully compatible with universal conditions for two-step RT-PCR. Just add TaqMan® Universal PCR Master Mix (with or without AmpErase® UNG) and your cDNA sample to generate sensitive, reproducible, and truly quantitative gene expression data on Applied Biosystems instruments including the Applied Biosystems 7900HT, 7300, 7500 Real-Time PCR Systems, and the 7000 and 7700 Sequence Detection Systems.

Flexible Offering

We build each endogenous control using our proven 5' nuclease chemistry. For maximum flexibility, you can choose between two different reporter dyes and two quenchers:
• A FAM™ dye-labeled TaqMan® MGB probe (250nM, final concentration) and two unlabeled PCR primers (900nM each)
• A VIC® dye-labeled TaqMan® MGB probe (250nM, final concentration) and two unlabeled PCR primers (150nM each y primer limited)
• A VIC® dye-labeled TAMRA™ probe (250nM, final concentration) and two unlabeled PCR primers (150nM each y primer limited)

Choosing the Right Endogenous Control

Endogenous controls can normalize the expression levels of target genes by correcting differences in the amount of cDNA that is loaded into PCR reaction wells. For best results, verify that the endogenous control is consistently expressed in the sample set to be tested. Endogenous control expression must be uniform across all samples in the study. For multiplexing, ensure that the gene expression level of the endogenous control is greater than that of the target.

Multiplex vs. Singleplex PCR

All TaqMan® Endogenous Controls that contain probes labeled with the VIC® reporter dye are primer limited. This allows multiplexing of TaqMan® Endogenous Controls with target gene expression assays, provided that the control gene is more abundantly expressed than the target gene. All TaqMan® Endogenous Controls that contain probes labeled with the FAM™ reporter dye are not primer limited and are not intended for multiplexing .

For Research Use Only. Not for use in diagnostic procedures.

Human PPIA (Cyclophilin A) Endogenous Control (VIC™/TAMRA™ probe, primer limited) (Applied Biosystems™)

The Applied Biosystems® Human PPIA (cyclophilin A) Endogenous Control (VIC® ⁄ TAMRA Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when used with other TaqMan® gene expression assays. Probe is labeled with VIC™ dye - TAMRA™ dye and the primers are limited. Can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with Inventoried and Made to Order TaqMan® Gene Expression Assays, Custom TaqMan® Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes.

Assay Details:

Gene Symbol: PPIA
RefSeq: NM_021130.3
Probe Exon Location:1
Amplicon Size: 138

TaqMan® Endogenous Controls

Eliminate months of assay design, formulation, and testing by using TaqMan® Endogenous Controls as your controls to quantify gene expression. This convenient collection of pre-designed probe and primer sets enables you to normalize the amount of sample RNA or DNA in a reaction.

Complete Solution for Quantitative Gene Expression

Having a hard time deciding what controls to use to quantify gene expression — even with detailed information on biological systems? Now, with TaqMan® Endogenous Controls, you can avoid all the trial-and-error of selecting controls for most common human, mouse, rat, and eukaryotic genes.

Simple to Use

All components of the TaqMan® Endogenous Controls are QC tested, formulated into a single 20X mix, and functionally tested. The controls are not only simple to use, but they are also fully compatible with universal conditions for two-step RT-PCR. Just add TaqMan® Universal PCR Master Mix (with or without AmpErase® UNG) and your cDNA sample to generate sensitive, reproducible, and truly quantitative gene expression data on Applied Biosystems instruments including the Applied Biosystems 7900HT, 7300, 7500 Real-Time PCR Systems, and the 7000 and 7700 Sequence Detection Systems.

Flexible Offering

We build each endogenous control using our proven 5' nuclease chemistry. For maximum flexibility, you can choose between two different reporter dyes and two quenchers:
• A FAM™ dye-labeled TaqMan® MGB probe (250nM, final concentration) and two unlabeled PCR primers (900nM each)
• A VIC® dye-labeled TaqMan® MGB probe (250nM, final concentration) and two unlabeled PCR primers (150nM each y primer limited)
• A VIC® dye-labeled TAMRA™ probe (250nM, final concentration) and two unlabeled PCR primers (150nM each y primer limited)

Choosing the Right Endogenous Control

Endogenous controls can normalize the expression levels of target genes by correcting differences in the amount of cDNA that is loaded into PCR reaction wells. For best results, verify that the endogenous control is consistently expressed in the sample set to be tested. Endogenous control expression must be uniform across all samples in the study. For multiplexing, ensure that the gene expression level of the endogenous control is greater than that of the target.

Multiplex vs. Singleplex PCR

All TaqMan® Endogenous Controls that contain probes labeled with the VIC® reporter dye are primer limited. This allows multiplexing of TaqMan® Endogenous Controls with target gene expression assays, provided that the control gene is more abundantly expressed than the target gene. All TaqMan® Endogenous Controls that contain probes labeled with the FAM™ reporter dye are not primer limited and are not intended for multiplexing.

For Research Use Only. Not for use in diagnostic procedures.

VetMAX™ Xeno™ Internal Positive Control - VIC™ Assay (Applied Biosystems™)

The VetMAX™ Xeno™ Internal Positive Control (IPC) - VIC™ Assay is a primer-probe mix that detects the Xeno internal positive control. The resultant Xeno data is used to determine the validity of diagnostic test results. The Xeno IPC assay is introduced during the qPCR preparation step and carried through the animal health PCR workflow.

Features of the VetMAX Xeno IPC - VIC Assay include:
• Provides confidence that qPCR test results are accurate and actionable
• Easily integrates into any workflow
• Greatly reduces the likelihood of false negatives

The VetMAX Xeno IPC - VIC Assay comes in a 25X concentration and easily integrates into animal health PCR workflows, regardless of the target assay, master mix, or sample preparation reagents already in place. Coupled with VetMAX™ Xeno Internal Positive Control RNA or VetMAX™ Xeno Internal Positive Control DNA, our proprietary design offers a verification layer to help ensure the qPCR test results are accurate and actionable by greatly reducing the likelihood of false negatives.

Xeno IPC assays are included in most VetMAX kits and have been successfully benchmarked against millions of genomes including those relevant to animal health. A VetMAX™ Xeno™ Internal Positive Control - LIZ™ Assay (Cat. No. A29766) is also available.

Human B2M (Beta-2-Microglobulin) Endogenous Control (VIC™/MGB probe, primer limited) (Applied Biosystems™)

The Applied Biosystems® Human B2M (beta-2-microglobulin) Endogenous Control (VIC® ⁄MGB Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when used with other gene expression assays. Probe is labeled with VIC™ dye - MGB and the primers are limited. Can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with Inventoried and Non-Inventoried TaqMan® Gene Expression Assays, Custom TaqMan® Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes.

Assay Details:

Gene Symbol: B2M
RefSeq: NM_004048.2
Probe Exon Location:2-3
Amplicon Size: 75
Corresponding TaqMan Assay ID: Hs99999907_m1

TaqMan® Endogenous Controls

Eliminate months of assay design, formulation, and testing by using TaqMan® Endogenous Controls as your controls to quantify gene expression. This convenient collection of pre-designed probe and primer sets enables you to normalize the amount of sample RNA or DNA in a reaction.

Complete Solution for Quantitative Gene Expression

Having a hard time deciding what controls to use to quantify gene expression — even with detailed information on biological systems? Now, with TaqMan® Endogenous Controls, you can avoid all the trial-and-error of selecting controls for most common human, mouse, rat, and eukaryotic genes.

Simple to Use

All components of the TaqMan® Endogenous Controls are QC tested, formulated into a single 20X mix, and functionally tested. The controls are not only simple to use, but they are also fully compatible with universal conditions for two-step RT-PCR. Just add TaqMan® Universal PCR Master Mix (with or without AmpErase® UNG) and your cDNA sample to generate sensitive, reproducible, and truly quantitative gene expression data on Applied Biosystems instruments including the Applied Biosystems 7900HT, 7300, 7500 Real-Time PCR Systems, and the 7000 and 7700 Sequence Detection Systems.

Flexible Offering

We build each endogenous control using our proven 5' nuclease chemistry. For maximum flexibility, you can choose between two different reporter dyes and two quenchers:
• A FAM™ dye-labeled TaqMan® MGB probe (250nM, final concentration) and two unlabeled PCR primers (900nM each)
• A VIC® dye-labeled TaqMan® MGB probe (250nM, final concentration) and two unlabeled PCR primers (150nM each y primer limited)
• A VIC® dye-labeled TAMRA™ probe (250nM, final concentration) and two unlabeled PCR primers (150nM each y primer limited)

Choosing the Right Endogenous Control

Endogenous controls can normalize the expression levels of target genes by correcting differences in the amount of cDNA that is loaded into PCR reaction wells. For best results, verify that the endogenous control is consistently expressed in the sample set to be tested. Endogenous control expression must be uniform across all samples in the study. For multiplexing, ensure that the gene expression level of the endogenous control is greater than that of the target.

Multiplex vs. Singleplex PCR

All TaqMan® Endogenous Controls that contain probes labeled with the VIC® reporter dye are primer limited. This allows multiplexing of TaqMan® Endogenous Controls with target gene expression assays, provided that the control gene is more abundantly expressed than the target gene. All TaqMan® Endogenous Controls that contain probes labeled with the FAM™ reporter dye are not primer limited and are not intended for multiplexing .

For Research Use Only. Not for use in diagnostic procedures.

VetMAX™ Xeno™ Internal Positive Control DNA (Applied Biosystems™)

VetMAX™ Xeno™ Internal Positive Control (IPC) DNA serves as an internal positive control for the DNA purification process and helps monitor for the presence of PCR inhibitors in animal health molecular detection workflows. This is of particular importance when working with sample types that have a high level of inhibitors such as feces. Xeno IPC DNA is introduced at the nucleic acid isolation/preparation step and carried through the animal health qPCR workflow.

Features of VetMAX Xeno IPC DNA include:
• Provides confidence that qPCR test results are accurate and actionable
• Easily integrates into any workflow
• Greatly reduces the likelihood of false negatives

VetMAX Xeno IPC DNA comes in a concentration of 10,000 copies/µL and easily integrates into animal health PCR workflows, regardless of the target assay, master mix, or sample preparation reagents already in place. Coupled with a Xeno internal positive control assay, our proprietary design offers a verification layer to help ensure the PCR test results are accurate and actionable by greatly reducing the likelihood of false negatives.

Xeno IPC DNA is a component of our licensed VetMAX Gold detection kits, recommended in the American Association of Veterinary Diagnosticians guidelines, and has been successfully benchmarked against millions of genomes including those relevant to animal health. Xeno IPC RNA is also available (Cat. No. A29761).