Shop All Control Primers, Primer⁄Probe Sets & Templates

TaqMan™ OpenArray™ Genotyping Barcode Panel 60, QuantStudio™ 12K Flex plate (Applied Biosystems™)

The TaqMan OpenArray Genotyping Barcode Panel 60 consists of 3 Y-markers and 57 autosomal SNPs that allow for unique identification of individual samples for biorepository applications. A total of 47 samples and 1 no template control can be run with this panel.

Markers were selected based on high minor allele frequency in multiple ethnic populations, representation on high throughput genotyping, and robust genotyping performance. These markers allow unique identification of individuals within sample collections. The panel allows unique identification of individuals throughout experimental processes by providing both sample identity and gender checks in the event of sample mix-ups or labeling mistakes. It also serves as an internal control for genome-wide association studies by containing markers from large microarrays to allow matching of sample genotypes directly from the panel to a GWAS array.

This panel is intended for use with the QuantStudio 12K Flex Real-time PCR system which can cycle and image 4 pates simultaneously, providing high-throughput results with ease.

TaqMan™ OpenArray™ HS Endogenous Control Panel, QuantStudio™ 12K Flex plate (Applied Biosystems™)

The TaqMan® OpenArray® HS Endogenous Control Panel, QuantStudio™ 12K Flex is a fixed-content panel containing 56 TaqMan® SNP Gene Expression Assays offering a variety of different functional qualities. These 56 genes are expressed constitutively in most cells since they are genes that encode proteins that are necessary for cell functionality. This consistency in expression means that the housekeeping genes are ideal as standards in comparative expression analysis.

This OpenArray® plate is designed to function as a quality control panel for testing sample preparation methods prior to using custom TaqMan® OpenArray® Gene Expression QuantStudio™ 12K Flex plates.

For Research Use Only. Not for use in diagnostics procedures.

VetMAX™ Xeno™ Internal Positive Control RNA (Applied Biosystems™)

VetMAX™ Xeno™ Internal Positive Control (IPC) RNA serves as an internal positive control for the RNA purification process and helps monitor for the presence of PCR inhibitors in animal health molecular detection workflows. This is of particular importance when working with sample types that have a high level of inhibitors such as oral fluids. Xeno IPC RNA is introduced at the nucleic acid isolation/preparation step and carried through the animal health PCR workflow.

Features of VetMAX Xeno IPC RNA include:
• Provides confidence that PCR test results are accurate and actionable
• Easily integrates into any workflow
• Greatly reduces the likelihood of false negatives

VetMAX Xeno IPC RNA comes in a concentration of 10,000 copies/µL and easily integrates into animal health PCR workflows, regardless of the target assay, master mix, or sample preparation reagents already in place. Coupled with a Xeno internal positive control assay, our proprietary design offers a verification layer to help ensure the qPCR test results are accurate and actionable by greatly reducing the likelihood of false negatives.

Xeno IPC RNA is a component of our licensed VetMAX Gold detection kits, is recommended in the American Association of Veterinary Diagnosticians guidelines, and has been successfully benchmarked against millions of genomes including those relevant to animal health. Xeno IPC DNA is also available (Cat. No. A29762).

Rat GAPD (GAPDH) Endogenous Control (VIC™/MGB probe, primer limited) (Applied Biosystems™)

The Applied Biosystems® Rat GAPD (GAPDH) Endogenous Control (VIC® ⁄ MGB Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when used with other gene expression assays. Probe is labeled with VIC® dye - MGB and the primers are limited. It can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with TaqMan® Gene Expression Assays, TaqMan® Custom Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes.

Assay Details:

Gene Symbol: GAPDH
RefSeq: NM_017008.3
Probe Exon Location: 3
Amplicon Size: 87
Corresponding TaqMan Assay ID: Rn99999916_s1

TaqMan® Endogenous Controls

Eliminate months of assay design, formulation, and testing by using TaqMan® Endogenous Controls as your controls to quantify gene expression. This convenient collection of pre-designed probe and primer sets enables you to normalize the amount of sample RNA or DNA in a reaction.

Complete Solution for Quantitative Gene Expression

Having a hard time deciding what controls to use to quantify gene expression — even with detailed information on biological systems? Now, with TaqMan® Endogenous Controls, you can avoid all the trial-and-error of selecting controls for most common human, mouse, rat, and eukaryotic genes.

Simple to Use

All components of the TaqMan® Endogenous Controls are QC tested, formulated into a single 20X mix, and functionally tested. The controls are not only simple to use, but they are also fully compatible with universal conditions for two-step RT-PCR. Just add TaqMan® Universal PCR Master Mix (with or without AmpErase® UNG) and your cDNA sample to generate sensitive, reproducible, and truly quantitative gene expression data on Applied Biosystems instruments including the Applied Biosystems 7900HT, 7300, 7500 Real-Time PCR Systems, and the 7000 and 7700 Sequence Detection Systems.

Flexible Offering

We build each endogenous control using our proven 5' nuclease chemistry. For maximum flexibility, you can choose between two different reporter dyes and two quenchers:
• A FAM™ dye-labeled TaqMan® MGB probe (250nM, final concentration) and two unlabeled PCR primers (900nM each)
• A VIC® dye-labeled TaqMan® MGB probe (250nM, final concentration) and two unlabeled PCR primers (150nM each y primer limited)
• A VIC® dye-labeled TAMRA™ probe (250nM, final concentration) and two unlabeled PCR primers (150nM each y primer limited)

Choosing the Right Endogenous Control

Endogenous controls can normalize the expression levels of target genes by correcting differences in the amount of cDNA that is loaded into PCR reaction wells. For best results, verify that the endogenous control is consistently expressed in the sample set to be tested. Endogenous control expression must be uniform across all samples in the study. For multiplexing, ensure that the gene expression level of the endogenous control is greater than that of the target.

Multiplex vs. Singleplex PCR

All TaqMan® Endogenous Controls that contain probes labeled with the VIC® reporter dye are primer limited. This allows multiplexing of TaqMan® Endogenous Controls with target gene expression assays, provided that the control gene is more abundantly expressed than the target gene. All TaqMan® Endogenous Controls that contain probes labeled with the FAM™ reporter dye are not primer limited and are not intended for multiplexing .

For Research Use Only. Not for use in diagnostic procedures.

VetMAX™ M. hyopneumoniae Controls (positive internal DNA control) (Applied Biosystems™)

VetMAX™ M. hyopneumoniae Controls is a mixture of synthetic Mycoplasma hyopneumoniae DNA and Xeno™ DNA Internal Positive Control, used as a positive control mix for real-time PCR amplification of M. hyopneumoniae DNA.

For Research Use Only. Not for use in diagnostics procedures.

VetMAX™ Xeno™ Internal Positive Control DNA (Applied Biosystems™)

VetMAX™ Xeno™ Internal Positive Control (IPC) DNA serves as an internal positive control for the DNA purification process and helps monitor for the presence of PCR inhibitors in animal health molecular detection workflows. This is of particular importance when working with sample types that have a high level of inhibitors such as feces. Xeno IPC DNA is introduced at the nucleic acid isolation/preparation step and carried through the animal health qPCR workflow.

Features of VetMAX Xeno IPC DNA include:
• Provides confidence that qPCR test results are accurate and actionable
• Easily integrates into any workflow
• Greatly reduces the likelihood of false negatives

VetMAX Xeno IPC DNA comes in a concentration of 10,000 copies/µL and easily integrates into animal health PCR workflows, regardless of the target assay, master mix, or sample preparation reagents already in place. Coupled with a Xeno internal positive control assay, our proprietary design offers a verification layer to help ensure the PCR test results are accurate and actionable by greatly reducing the likelihood of false negatives.

Xeno IPC DNA is a component of our licensed VetMAX Gold detection kits, recommended in the American Association of Veterinary Diagnosticians guidelines, and has been successfully benchmarked against millions of genomes including those relevant to animal health. Xeno IPC RNA is also available (Cat. No. A29761).

Mouse GAPD (GAPDH) Endogenous Control (VIC™/MGB probe, primer limited) (Applied Biosystems™)

The Applied Biosystems® Mouse GAPD (GAPDH) Endogenous Control (VIC®⁄MGB Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when used with other gene expression assays. Probe is labeled with VIC® dye - MGB and the primers are limited. Can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with Inventoried and Non-Inventoried TaqMan® Gene Expression Assays, TaqMan® Custom Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes.

Assay Details:

Gene Symbol: GAPDH
RefSeq: NM_008084.2
Probe Exon Location: 3
Amplicon Size: 107
Corresponding TaqMan Assay ID: Mm99999915_g1

TaqMan® Endogenous Controls

Eliminate months of assay design, formulation, and testing by using TaqMan® Endogenous Controls as your controls to quantify gene expression. This convenient collection of pre-designed probe and primer sets enables you to normalize the amount of sample RNA or DNA in a reaction.

Complete Solution for Quantitative Gene Expression

Having a hard time deciding what controls to use to quantify gene expression — even with detailed information on biological systems? Now, with TaqMan® Endogenous Controls, you can avoid all the trial-and-error of selecting controls for most common human, mouse, rat, and eukaryotic genes.

Simple to Use

All components of the TaqMan® Endogenous Controls are QC tested, formulated into a single 20X mix, and functionally tested. The controls are not only simple to use, but they are also fully compatible with universal conditions for two-step RT-PCR. Just add TaqMan® Universal PCR Master Mix (with or without AmpErase® UNG) and your cDNA sample to generate sensitive, reproducible, and truly quantitative gene expression data on Applied Biosystems instruments including the Applied Biosystems 7900HT, 7300, 7500 Real-Time PCR Systems, and the 7000 and 7700 Sequence Detection Systems.

Flexible Offering

We build each endogenous control using our proven 5' nuclease chemistry. For maximum flexibility, you can choose between two different reporter dyes and two quenchers:
• A FAM™ dye-labeled TaqMan® MGB probe (250nM, final concentration) and two unlabeled PCR primers (900nM each)
• A VIC® dye-labeled TaqMan® MGB probe (250nM, final concentration) and two unlabeled PCR primers (150nM each y primer limited)
• A VIC® dye-labeled TAMRA™ probe (250nM, final concentration) and two unlabeled PCR primers (150nM each y primer limited)

Choosing the Right Endogenous Control

Endogenous controls can normalize the expression levels of target genes by correcting differences in the amount of cDNA that is loaded into PCR reaction wells. For best results, verify that the endogenous control is consistently expressed in the sample set to be tested. Endogenous control expression must be uniform across all samples in the study. For multiplexing, ensure that the gene expression level of the endogenous control is greater than that of the target.

Multiplex vs. Singleplex PCR

All TaqMan® Endogenous Controls that contain probes labeled with the VIC® reporter dye are primer limited. This allows multiplexing of TaqMan® Endogenous Controls with target gene expression assays, provided that the control gene is more abundantly expressed than the target gene. All TaqMan® Endogenous Controls that contain probes labeled with the FAM™ reporter dye are not primer limited and are not intended for multiplexing .

For Research Use Only. Not for use in diagnostic procedures.

Human RPLPO (Large Ribosomal Protein) Endogenous Control, VIC™/MGB probe, primer limited (Applied Biosystems™)

Human RPLPO (Large Ribosomal Protein) Endogenous Control allows relative gene expression quantification in cDNA samples when used with other gene expression assays. The probe is labeled with VIC™ dye-MGB, and the primers are limited. It can be used for multiplex or singleplex PCR reactions. This endogenous control is designed to be used with TaqMan® gene expression assays or TaqMan® primers/probes.

Assay Details:

Gene Symbol: RPLPO
RefSeq: NM_001002.3
Probe Exon Location:3
Amplicon Size: 105
Corresponding TaqMan Assay ID: Hs99999902_m1

TaqMan® Endogenous Controls

Eliminate months of assay design, formulation, and testing by using TaqMan® endogenous controls to quantify gene expression. This convenient collection of pre-designed primer/probe sets enables you to normalize the amount of sample RNA or DNA in a reaction.

Complete Solution for Quantitative Gene Expression

Having a hard time deciding what controls to use to quantify gene expression—even with detailed information on biological systems? Now, with TaqMan® endogenous controls, you can avoid all the trial-and-error of selecting controls for the most common human, mouse, rat, and eukaryotic genes.

Simple to Use

All components of the TaqMan® endogenous controls are QC tested, formulated into a single 20X mix, and functionally tested. The controls are not only simple to use, but they are also fully compatible with universal conditions for two-step RT-PCR. Just add TaqMan® Universal PCR Master Mix (with or without AmpErase® UNG) and your cDNA sample to generate sensitive, reproducible, and truly quantitative gene expression data on Applied Biosystems instruments including the Applied Biosystems 7900HT, 7300, 7500 Real-Time PCR Systems, and the 7000 and 7700 Sequence Detection Systems.

Flexible Offering

We build each endogenous control using our proven 5' nuclease chemistry. For maximum flexibility, you can choose between two different reporter dyes and two quenchers:
• A FAM™ dye-labeled TaqMan® MGB probe (250nM, final concentration) and two unlabeled PCR primers (900nM each)
• A VIC® dye-labeled TaqMan® MGB probe (250 nM, final concentration) and two unlabeled PCR primers (150 nM each, primer limited)
• A VIC® dye-labeled TAMRA™ probe (250nM, final concentration) and two unlabeled PCR primers (150 nM each, primer limited)

Choosing the Right Endogenous Control

Endogenous controls can normalize the expression levels of target genes by correcting differences in the amount of cDNA that is loaded into PCR reaction wells. For best results, verify that the endogenous control is consistently expressed in the sample set to be tested. Endogenous control expression must be uniform across all samples in the study. For multiplexing, ensure that the gene expression level of the endogenous control is greater than that of the target.

Multiplex vs. Singleplex PCR

All TaqMan® endogenous controls that contain probes labeled with the VIC® reporter dye are primer limited. This allows multiplexing of TaqMan® endogenous controls with target gene expression assays, provided that the control gene is more abundantly expressed than the target gene. All TaqMan® endogenous controls that contain probes labeled with the FAM™ reporter dye are not primer limited and are not intended for multiplexing.

TaqMan™ DNA Template Reagents (Applied Biosystems™)

The TaqMan® DNA Template Reagents provide the necessary components to perform a standard-dilution series with the beta-actin gene using nucleic acid from human cells. TaqMan® beta-actin Template Reagents can be used with TaqMan® PCR Core Reagents or SYBR® Green PCR Core Reagents for polymerase chain reaction (PCR).

• Pre-designed primers and TaqMan® probe eliminate assay design.
• Rapid assay development guidelines can minimize optimization time.
• Separate components provide flexibility in assay set-up.

Comprehensive Guidelines Included
We have developed a comprehensive set of guidelines to ensure success when using Applied Biosystems® Real-Time PCR reagents and instrumentation. These guidelines are simple and easy to follow. Many traditional variables, such as magnesium chloride concentration and the thermal cycling protocol itself, have been standardized, greatly reducing assay development time.

Normalization Built-In
All our Real-Time PCR reagent kits contain a passive internal reference to normalize non-PCR related fluorescence fluctuations. Normalizing with a passive internal reference minimizes well-to-well variability that can result from a variety of causes, such as pipetting errors and sample evaporation. The passive reference is also essential for accurate results when multiple probes (with different reporter dyes) are combined in a single tube.

For Research Use Only. Not for use in diagnostics procedures.

Lambda DNA (Thermo Scientific™)

Thermo Scientific Lambda is a temperate Escherichia coli bacteriophage. The virion DNA is linear and double-stranded (48502 bp) with 12 bp single-stranded complementary 5’-ends. After the phage particle injects its chromosome into the cell, the chromosome circularizes by end joining. In the lytic pathway, phage genes encoding replication, lysis, and virion proteins are expressed. The chromosome replicates, and the replicas are cleaved and packaged into progeny phage particles. In the lysogenic pathway, phage gene expression is repressed, and the circular chromosome inserts into the bacterial chromosome by recombination.

Features
Phage lambda DNA is a common substrate for restriction endonucleases and for generating DNA size marker fragments. For large scale isolation of phage DNA, cI857Sam7, a mutant carrying four known mutations, is used. The DNA sequence used to construct a phage alpha restriction map includes these mutations.

Applications

• Activity and specificity assays of restriction enzymes
• Preparation of DNA molecular weight standards
• Cloning

GenBank/EMBL Accession Numbers
NC_001416, M17233, M24325, V00636, X00906

TaqMan™ Copy Number Reference Assay, mouse, Tfrc (Applied Biosystems™)

Mouse TaqMan® Copy Number Reference Assays are run with predesigned, Custom Plus, and Custom TaqMan® Copy Number Assays in a duplex, real-time PCR reaction to detect and measure copy number variations in the mouse genome. Mouse TaqMan® Copy Number Reference Assays are also compatible with the common vector marker and reporter gene predesigned TaqMan® Copy Number Assays. Two options for genes that can be used as endogenous reference genes in mice are offered: Tfrc and Tert. TaqMan® Copy Number Reference Assays are designed to unique genomic sequences in the mouse reference genome assembly (NCBI Build 37/mm9) and are required for relative quantitation of copy number targets.

TaqMan® Copy Number Assays quantitate the gene of interest and normalize to an endogenous reference gene known to be present in two copies in a diploid genome. Please note that TaqMan® Copy Number Reference Assays are species-specific.

Tfrc: The Standard Reference Gene Option
TaqMan® Copy Number Reference Assays have a VIC® dye-labeled TAMRA™ probe and reference sequence-specific forward and reverse primers. The assays are not primer limited.

TaqMan® Copy Number Reference Assay, Mouse, Tfrc is recommended as the standard reference assay for copy number analysis in mice. This assay detects the transferrin receptor gene (Tfrc) on chromosome 16, cytoband 16qB3. The assay location is chr.16:32626732 on NCBI build 37. It has a 91 bp amplicon that maps within exon 17 of the Tfrc gene.

TaqMan® Copy Number Reference Assay, Mouse, Tert is an alternative reference assay; it is recommended in the event that the Tfrc assay functions poorly with a sample because of chromosomal aberrations or other issues. The assay targets the telomerase reverse transcriptase (Tert) gene on chromosome 13, cytoband 13qC1. The assay location is chr.13:73778992 on NCBI build 37. It has a 96 bp amplicon that maps within intron 8 of the Tert gene.

Simplest Workflow Available
TaqMan® Copy Number Assays have the simplest workflow of all currently available CNV analysis methods. The test assay (FAM™ dye labeled), the reference assay (VIC® dye labeled), your sample DNA, and TaqMan® Master Mix are combined and then run on an Applied Biosystems® Real-Time PCR System using the standard TaqMan® Copy Number Assay protocol. On average, set-up to primary analysis takes only 3-4 hours (including an approximately 2 hour PCR run).

Powerful Data Analysis Software
CopyCaller® Software was developed specifically for TaqMan® Copy Number Assay data analysis. This free, easy-to-use software utilizes a graphical interface that quickly calculates the possible copy numbers for a set of samples in a run. It also gives a confidence value for each copy number call, and has outlier functionality.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

VetMAX™ Xeno™ Internal Positive Control - LIZ™ Assay (Applied Biosystems™)

The VetMAX™ Xeno™ Internal Positive Control (IPC) - LIZ™ Assay is a primer-probe mix that detects the Xeno internal positive control. The resultant Xeno data is used to determine the validity of diagnostic test results. The Xeno IPC LIZ assay is introduced during the qPCR preparation step and carried through the animal health PCR workflow.

Features of the VetMAX Xeno IPC - LIZ Assay include:
• Provides confidence that qPCR test results are accurate and actionable
• Easily integrates into any workflow
• Greatly reduces the likelihood of false negatives

The VetMAX Xeno IPC - LIZ Assay comes in a 25X concentration and easily integrates into animal health PCR workflows, regardless of the target assay, master mix, or sample preparation reagents already in place. Coupled with VetMAX™ Xeno™ Internal Positive Control RNA or VetMAX™ Xeno™ Internal Positive Control DNA, our proprietary design offers a verification layer to help ensure the qPCR test results are accurate and actionable by greatly reducing the likelihood of false negatives.

Xeno IPC assays are included in most VetMAX kits and have been successfully benchmarked against millions of genomes including those relevant to animal health. A VetMAX™ Xeno™ Internal Positive Control - VIC™ Assay (Cat. No. A29767) is also available.

TaqMan™ Control Genomic DNA (human) (Applied Biosystems™)

Human Genomic DNA Control provided at a convenient PCR-ready concentration.

Applied Biosystems® TaqMan® Control Genomic DNA (Human) is conveniently packaged in a PCR-ready concentration for use as a control template in PCR reactions. The Human gDNA (male) is provided in two tubes (100 μL each) at a concentration of 10 ng⁄μL. TaqMan® Control Genomic Human DNA is also included in several Applied Biosystems® real-time PCR kits.

TaqMan™ Copy Number Reference Assay, human, TERT (Applied Biosystems™)

Human TaqMan® Copy Number Reference Assays are run with human TaqMan® Copy Number Assays in a duplex real-time PCR reaction to detect and measure copy number variations (CNVs) and smaller regions in the human genome. Two options for genes that can be used as endogenous reference genes in humans are offered: RNase P and TERT. TaqMan® Copy Number Reference Assays are designed to unique genomic sequences in the reference genome assembly (Build 37/hg19) and are required for relative quantitation of copy number targets.

TaqMan® Copy Number Assays quantitate the gene of interest and normalize to an endogenous reference gene known to be present in two copies in a diploid genome. Please note that TaqMan® Copy Number Reference Assays are species specific.

TERT: The Alternative Reference Gene Option
TaqMan® Copy Number Reference Assays have a VIC® dye–labeled TAMRA™ probe and reference sequence–specific forward and reverse primers. The assays are not primer-limited.

TaqMan® Copy Number Reference Assay RNase P is recommended as the standard reference assay for copy number analysis. This assay detects the Ribonuclease P RNA component H1 (H1RNA) gene (RPPH1) on chromosome 14, cytoband 14q11.2. The assay location is chr.14:20811565 on NCBI build 37. It has an 87 bp amplicon that maps within the single exon RPPH1 gene.

TaqMan® Copy Number Reference Assay TERT is an alternative reference assay; it is recommended in the event that the RNase P assay functions poorly with a sample because of chromosomal aberrations or other issues. This assay targets the telomerase reverse transcriptase (TERT) gene located on chromosome 5, cytoband 5p15.33. The assay location is chr.5:1253373 on NCBI build 37. It has an 88 bp amplicon that maps within exon 16 of the TERT gene.

Simplest Copy Number Analysis Workflow
TaqMan® Copy Number Assays have the simplest workflow of all currently available copy number analysis methods. The test assay (FAM™ dye–labeled), the reference assay (VIC® dye–labeled), your sample DNA, and TaqMan® Master Mix are combined and then run on an Applied Biosystems® real-time PCR system using the standard TaqMan® Copy Number Assay protocol. On average, setup to primary analysis takes only 3–4 hr (including an approximately 2 hour PCR run).

Powerful Data analysis Software
CopyCaller® Software was developed specifically for TaqMan® Copy Number Assay data analysis. This free, easy-to-use software utilizes a graphical interface that quickly calculates the possible copy numbers for a set of samples in a run. It also gives a confidence value for each copy number call, and has outlier functionality.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

Human GUSB (Beta Glucuronidase) Endogenous Control (VIC™/MGB probe, primer limited) (Applied Biosystems™)

The Applied Biosystems® Human GUSB (beta glucuronidase) Endogenous Control (VIC® ⁄ MGB Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when used with other gene expression assays. Probe is labeled with VIC™ dye - MGB and the primers are limited. Can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with Inventoried and Non-Inventoried TaqMan® Gene Expression Assays, Custom TaqMan® Gene Expression Assays, and⁄or Custom TaqMan® Primers and Probes.

Assay Details:

Gene Symbol: GUSB
RefSeq: NM_000181.1
Probe Exon Location:11-12
Amplicon Size: 81
Corresponding TaqMan Assay ID: Hs99999908_m1

TaqMan® Endogenous Controls

Eliminate months of assay design, formulation, and testing by using TaqMan® Endogenous Controls as your controls to quantify gene expression. This convenient collection of pre-designed probe and primer sets enables you to normalize the amount of sample RNA or DNA in a reaction.

Complete Solution for Quantitative Gene Expression

Having a hard time deciding what controls to use to quantify gene expression — even with detailed information on biological systems? Now, with TaqMan® Endogenous Controls, you can avoid all the trial-and-error of selecting controls for most common human, mouse, rat, and eukaryotic genes.

Simple to Use

All components of the TaqMan® Endogenous Controls are QC tested, formulated into a single 20X mix, and functionally tested. The controls are not only simple to use, but they are also fully compatible with universal conditions for two-step RT-PCR. Just add TaqMan® Universal PCR Master Mix (with or without AmpErase® UNG) and your cDNA sample to generate sensitive, reproducible, and truly quantitative gene expression data on Applied Biosystems instruments including the Applied Biosystems 7900HT, 7300, 7500 Real-Time PCR Systems, and the 7000 and 7700 Sequence Detection Systems.

Flexible Offering

We build each endogenous control using our proven 5' nuclease chemistry. For maximum flexibility, you can choose between two different reporter dyes and two quenchers:
• A FAM™ dye-labeled TaqMan® MGB probe (250nM, final concentration) and two unlabeled PCR primers (900nM each)
• A VIC® dye-labeled TaqMan® MGB probe (250nM, final concentration) and two unlabeled PCR primers (150nM each y primer limited)
• A VIC® dye-labeled TAMRA™ probe (250nM, final concentration) and two unlabeled PCR primers (150nM each y primer limited)

Choosing the Right Endogenous Control

Endogenous controls can normalize the expression levels of target genes by correcting differences in the amount of cDNA that is loaded into PCR reaction wells. For best results, verify that the endogenous control is consistently expressed in the sample set to be tested. Endogenous control expression must be uniform across all samples in the study. For multiplexing, ensure that the gene expression level of the endogenous control is greater than that of the target.

Multiplex vs. Singleplex PCR

All TaqMan® Endogenous Controls that contain probes labeled with the VIC® reporter dye are primer limited. This allows multiplexing of TaqMan® Endogenous Controls with target gene expression assays, provided that the control gene is more abundantly expressed than the target gene. All TaqMan® Endogenous Controls that contain probes labeled with the FAM™ reporter dye are not primer limited and are not intended for multiplexing .

For Research Use Only. Not for use in diagnostic procedures.