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FluoCells™ Prepared Slide #2 (BPAE cells with Mouse Anti-α-tubulin, BODIPY™ FL Goat Anti-Mouse IgG, Texas Red™-X Phalloidin, and DAPI) (Invitrogen™)
FluoCells prepared microscope slides contain multilabeled cell preparations for observation by fluorescence microscopy. FluoCells prepared slide #2 contains bovine pulmonary artery endothelial cells (BPAEC). Texas Red-X phalloidin is labeling the F-actin, anti—bovine α-tubulin mouse monoclonal 236-10501 in conjunction with BODIPY FL goat anti—mouse IgG antibody is labeling microtubules, and DAPI is labeling the nucleus.
The ViewRNA™ ISH Tissue Positive Control Kit (2-Plex) is designed for use with ViewRNA ISH Tissue 2-Plex Assays, QVT0012 or QVT0013. This positive control kit includes 4-6 µm thick rat kidney FFPE sections attached to positively charged slides as well as TYPE 1 rat Spp1 and TYPE 6 rat Ubc Probe Sets.
FluoCells™ Prepared Slide #3 (mouse kidney section with Alexa Fluor™ 488 WGA, Alexa Fluor™ 568 Phalloidin, and DAPI) (Invitrogen™)
The FluoCells prepared slide #3 is ideal for demonstrating the optical sectioning properties of confocal microscopes. Each slide contains a 16 µm cryostat section of mouse kidney stained with Alexa Fluor 488 wheat germ agglutinin (W-11261), Alexa Fluor 568 phalloidin (A-12380) and DAPI (D-1306). See also FluoCells prepared slide #4 (F-24631).
FluoCells™ Prepared Slide #1 (BPAE cells with MitoTracker™ Red CMXRos, Alexa Fluor™ 488 Phalloidin, and DAPI) (Invitrogen™)
FluoCells® prepared microscope slides contain fixed, multi-labeled cell preparations for observation by fluorescence microscopy. FluoCells® Prepared Slide #1 contains bovine pulmonary artery endothelial cells (BPAEC). MitoTracker® Red CMXRos was used to stain the mitochondria in the live cells, with accumulation dependent upon membrane potential. Following fixation and permeabilization, F-actin was stained with Alexa Fluor® 488 phalloidin, and the nuclei were counterstained with the blue-fluorescent DNA stain DAPI.