Shop All Microbiological Media

Luria Broth Base (Miller's LB Broth Base)™, powder Invitrogen™

Formulation per one liter:
10 g SELECT Peptone 140
5 g SELECT Yeast Extract
10 g sodium chloride

Yeast Nitrogen Base Invitrogen™

Yeast nitrogen base (YNB) is a base medium for preparation of minimal and synthetic defined yeast media. YNB contains ammonium sulfate but does not contain amino acids. Both bulk and convenient single-use pouches are available.

LB Broth Base Invitrogen™

LB Broth Base (Lennox L Broth Base) is recommended for the general maintenance and growth of E. coli.

LB Broth Base formulation
LB Broth Base has a lower salt concentration than Luria Broth Base. The formulation for 1 liter is: 10 g SELECT Peptone 140, 5 g SELECT Yeast Extract, and 5 g NaCl.

2-YT Broth Invitrogen™

Application(s): For growth of hosts for replication of M13 vectors.

Instructions for use:
1. Suspend 31 g in 1 L of demineralized water.
2. Autoclave for 15 min at 121°C.

Pichia EasyComp™ Transformation Kit Invitrogen™

The Pichia EasyComp™ Transformation Kit is a unique kit designed for the rapid preparation and transformation of competent Pichia pastoris cells. The Pichia EasyComp™ Transformation Kit offers several advantages over commonly used transformation procedures such as spheroplast formation:

• Competent cells can be frozen for future use, minimizing waste
• Competent cells can be prepared in less than 30 minutes
• Competent cell preparation and transformation solutions are optimized for use with Pichia pastoris are provided ready to use and are tested to ensure your results

LB Agar, powder (Lennox L agar) Invitrogen™

Formulation per one liter:
10 g SELECT Peptone 140
5 g SELECT Yeast Extract
5 g sodium chloride
12 g SELECT Agar

Select Agar™, powder Invitrogen™

An agent used for making solid microbiological media.

MagicMedia™ E. coli Expression Medium, powder Invitrogen™

MagicMedia E. coli Expression Medium is specifically designed to dramatically increase the yield of recombinant proteins in T7-regulated E. coli expression systems without monitoring culture OD (optical density) or adding inducer. The proprietary MagicMedia formulation enables E. coli growth to reach culture densities 3–10-fold higher than those achieved with traditional LB + IPTG methods, allowing you to:

• Achieve 3–10 times higher protein yields (Figure 1) with much less hands-on time
• Eliminate time-consuming OD monitoring steps—simply inoculate, grow the culture overnight, and harvest (Figure 2)
• Reduce culture volume to simplify handling and scale-up processes
• Ideal for simultaneous expression of multiple clones for high-throughput applications

MagicMedia E. coli Expression Medium, powder, consists of a premixed powder packaged in a convenient foil pouch, a proprietary component A (in foil pouch powder format), and component B (in buffer liquid format).

MagicMedia E. coli Expression Medium is also available in a ready-to-use, pre-sterilized liquid format (Cat. No. K6803).

MagicMedia™ E. coli Expression Medium Invitrogen™

MagicMedia™ E. coli Expression Medium is specifically designed to dramatically increase the yield of recombinant proteins in T7-regulated E. coli expression systems without monitoring culture O.D. (optical densities) or adding inducer. The proprietary MagicMedia™ formulation enables E. coli growth to reach culture densities 3-10-fold higher than those achieved with traditional LB + IPTG methods, allowing you to:

• Achieve 3-10 times higher protein yields (Figure 1) with much less hands-on time
• Eliminate time-consuming O.D. monitoring steps-simply inoculate prepared MagicMedia™ E. coli Expression Medium, grow the culture overnight, and harvest (Figure 2)
• Reduce culture volume to simplify handling and scale-up processes

In addition, MagicMedia™ E. coli Expression Medium is ideal for simultaneous expression of multiple clones for high-throughput applications.

imMedia™ Growth Medium, liquid, ampicillin Invitrogen™

imMedia Growth Medium is pre-mixed, pre-sterilized E. coli growth medium that can be prepared in just five minutes without autoclaving. imMedia Growth Medium contains everything you need to prepare low-salt LB liquid medium. You'll save hours of medium preparation time because there is:

• No weighing or mixing of media components
• No autoclaving
• No waiting for media to cool before adding antibiotics

Fast medium preparation
imMedia Growth Medium is fast food for E. coli. Simply mix with water in a clean flask, heat in a microwave oven, and in less than 5 minutes your medium is prepared.

The ultimate convenience
For your convenience, imMedia Growth Medium is available for preparing liquid medium or agar plates, with or without X-gal/IPTG. You have a choice of two antibiotics: ampicillin and kanamycin. imMedia Growth Medium is packaged in ready-to-use individual pouches. Each pouch contains sufficient reagents for preparing 200 mL of liquid media or 8-10 standard 100 mm2 agar plates.

Quality results
Each type of imMedia Growth Medium is extensively tested to ensure E. coli growth, antibiotic activity, and sterility. imMedia 'Blue' products (with X-gal/IPTG) are tested for efficient blue/white colony screening.

S.c. EasyComp™ Transformation Kit Invitrogen™

The S.c. EasyComp™ Transformation Kit is designed for rapid preparation of transformation-competent Saccharomyces cerevisiae cells. Advantages over commonly used transformation procedures such as spheroplast formation or LiCl methods include:

• Cells can be made competent in less than 30 minutes
• Competent cell preparation and transformation solutions are optimized for use with S. cerevisiae
are provided ready-to-use and tested to ensure successful yeast transformation
• Competent cells can be frozen for future use

imMedia™ Growth Medium, agar, ampicillin, X-gal/IPTG Invitrogen™

imMedia Growth Medium is pre-mixed, pre-sterilized E. coli growth medium that can be prepared in just five minutes without autoclaving. imMedia Growth Medium contains everything you need to prepare low-salt LB agar plates. You'll save hours of medium preparation time because there is:

• No weighing or mixing of media components
• No autoclaving
• No waiting for media to cool before adding antibiotics

Fast medium preparation
imMedia Growth Medium is fast food for E. coli. Simply mix with water in a clean flask, heat in a microwave oven, and in less than 5 minutes your medium is prepared.

The ultimate convenience
For your convenience, imMedia Growth Medium is available for preparing liquid medium or agar plates, with or without X-gal/IPTG. You have a choice of two antibiotics: ampicillin and kanamycin. imMedia Growth Medium is packaged in ready-to-use individual pouches. Each pouch contains sufficient reagents for preparing 200 mL of liquid media or 8-10 standard 100 mm2 agar plates.

Quality results
Each type of imMedia Growth Medium is extensively tested to ensure E. coli growth, antibiotic activity, and sterility. imMedia 'Blue' products (with X-gal/IPTG) are tested for efficient blue/white colony screening.

imMedia™ Growth Medium, agar, kanamycin Invitrogen™

imMedia Growth Medium is pre-mixed, pre-sterilized E. coli growth medium that can be prepared in just five minutes without autoclaving. imMedia Growth Medium contains everything you need to prepare low-salt LB agar plates. You'll save hours of medium preparation time because there is:

• No weighing or mixing of media components
• No autoclaving
• No waiting for media to cool before adding antibiotics

Fast medium preparation
imMedia Growth Medium is fast food for E. coli. Simply mix with water in a clean flask, heat in a microwave oven, and in less than 5 minutes your medium is prepared.

The ultimate convenience
For your convenience, imMedia Growth Medium is available for preparing liquid medium or agar plates, with or without X-gal/IPTG. You have a choice of two antibiotics: ampicillin and kanamycin. imMedia Growth Medium is packaged in ready-to-use individual pouches. Each pouch contains sufficient reagents for preparing 200 mL of liquid media or 8-10 standard 100 mm2 agar plates.

Quality results
Each type of imMedia Growth Medium is extensively tested to ensure E. coli growth, antibiotic activity, and sterility. imMedia 'Blue' products (with X-gal/IPTG) are tested for efficient blue/white colony screening.

PichiaPink™ Media Kit Invitrogen™

The PichiaPink™ Media Kit is a set of convenient prepackaged media pouches for use with the PichiaPink™ Yeast Expression System. The PichiaPink™ Media Kit contains media needed to grow Pichia pastoris strains from frozen stocks to cultures ready for transformation and selection. The PichiaPink™ Media Kit is an easy way get started using Pichia pastoris for protein production. Use the PichiaPink™ Media Kit to refill your PichiaPink™ Yeast Expression System Kits.

The PichiaPink™ Media Kit comes with prepackaged pouches for the following solutions. Each pouch makes 1 liter of solution. Media pouches should be kept dry and stored at room temperature.

MediaUsePouches
YP+agarFor plates to streak out colonies2 pouches
YPFor liquid cultures2 pouches
PAD (Pichia Adenine Dropout)+agarFor plates to grow PichiaPink™ recombinants2 pouches
YP+sorbital (YPS)For recovering PichiaPink™ transformants2 pouches
DextroseFor adding to YP and YP+agar1 pouch

Why Choose the PichiaPink™ Yeast Expression System?
The PichiaPink™ Yeast Expression System is based on the yeast Pichia pastoris. Advantages of Pichia pastoris include rapid growth, well-defined genetic background, simple media formulation, and easy handling. For over 30 years, Pichia pastoris has been used by labs around the world for producing hundreds of different proteins from many species including human (Ref 1, 2). The PichiaPink™ Yeast Expression System allows convenient and cost-effective protein production from small to large scales.

For information on obtaining a commercial-use license for the PichiaPink™Yeast Expression System, please inquire at outlicensing@lifetech.com.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Related Links
Learn more about the PichiaPink™ Yeast Expression System.
Learn more about our other protein expression systems.

References

1. Cereghino JL, Cregg JM. Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol Rev. 2000 Jan;24(1):45-66. [PubMed]
2. Cereghino GP, Cereghino JL, Ilgen C, Cregg JM. Production of recombinant proteins in fermenter cultures of the yeast Pichia pastoris. Curr Opin Biotechnol. 2002 Aug;13(4):329-32. [PubMed]

CT Supplement Applied Biosystems™

CT Supplement is added to standard Sorbitol MacConkey media (SMAC) to form CT-SMAC. CT-SMAC selects for E.coli 0157 after IMS.

This Product Contains: 1mg Cefixime powder and 50mg Potassium Tellurite powder.
Results per page
    spinner