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S.c. EasyComp™ Transformation Kit (Invitrogen™)

The S.c. EasyComp™ Transformation Kit is designed for rapid preparation of transformation-competent Saccharomyces cerevisiae cells. Advantages over commonly used transformation procedures such as spheroplast formation or LiCl methods include:

• Cells can be made competent in less than 30 minutes
• Competent cell preparation and transformation solutions are optimized for use with S. cerevisiae
are provided ready-to-use and tested to ensure successful yeast transformation
• Competent cells can be frozen for future use

CSM Media for Mav203 Yeast Cells (Invitrogen™)

The CSM Media for Mav203 Yeast Cells is a specifically designed media for making agar plates that enable the growth of Saccharomyces cerevisae MaV203 cells. This product comes with enough reagents to prepare 2 liters of solid media with the following components per liter:

• CSM (without tryptophan): 0.74 g
• Yeast Nitrogen Base: 6.66 g
• Ammonium Sulfate: 5 g
• Glucose: 20 g
• Agar: 20 g

Compatible with All MaV203 Applications
The CSM Media is optimized for the GeneArt® High-Order Genetic Assembly System, including the recovery for transformations of vectors once they have been adapted to yeast using the GeneArt® High-Order Vector Conversion Cassette. The media can also be used to grow MaV203 competent yeast cells, both library and sub-cloning scale (cat# 11281-011 and 11445-012).

Plate Preparation Protocol
For each liter, use one pouch of CSM -Trp Agar Yeast Media minus Glucose and 1 bottle of 20% Glucose. Dissolve the contents of the pouch in 900ml of distilled water and autoclave for 20 minutes on liquid cycle. Allow the solution to cool to 55°C and then add one bottle of 20% Glucose (100ml). Mix well and pour plates.

imMedia™ Growth Medium, agar, ampicillin (Invitrogen™)

imMedia Growth Medium is pre-mixed, pre-sterilized E. coli growth medium that can be prepared in just five minutes without autoclaving. imMedia Growth Medium contains everything you need to prepare low-salt LB agar plates. You'll save hours of medium preparation time because there is:

• No weighing or mixing of media components
• No autoclaving
• No waiting for media to cool before adding antibiotics

Fast medium preparation
imMedia Growth Medium is fast food for E. coli. Simply mix with water in a clean flask, heat in a microwave oven, and in less than 5 minutes your medium is prepared.

The ultimate convenience
For your convenience, imMedia Growth Medium is available for preparing liquid medium or agar plates, with or without X-gal/IPTG. You have a choice of two antibiotics: ampicillin and kanamycin. imMedia Growth Medium is packaged in ready-to-use individual pouches. Each pouch contains sufficient reagents for preparing 200 mL of liquid media or 8-10 standard 100 mm2 agar plates.

Quality results
Each type of imMedia Growth Medium is extensively tested to ensure E. coli growth, antibiotic activity, and sterility. imMedia "Blue" products (with X-gal/IPTG) are tested for efficient blue/white colony screening.

M9 Minimal Salts (2X) (Gibco™)

Gibco® M9 Minimal Salts (2X) is a ready-to-use liquid growth media used for bacterial culture. Our filter-sterilized Gibco® M9 Minimal Salts (2X) can be used for nearly all research applications that require a liquid bacterial culturing step such as protein expression, cloning, and expansion.
• Gibco® microbiological media
• Convenient ready-to-use liquid format
• 1 L size available

M9 Minimal Salts (2X) Recipe
M9 media is a commonly used bacterial media for cultivation and maintenance of Escherichia coli (E. coli) strains. Our Gibco® M9 Minimal Salts (2X) is a concentrated base formulation that can be supplemented with various carbon sources and amino acids to make a complete M9 medium.

Formulation per One Liter:
25.6 g   Sodium phosphate (dibasic) heptahydrate
6 g   Monopotassium phosphate
1 g   Sodium chloride
2 g   Ammonium chloride

Ready-to-Use in Optimized Gibco® Media Bottles
Each bottle of Gibco® M9 Minimal Salts (2X) is filter-sterilized and ready-to-use in ergonomic Gibco® media bottles with angled openings for simplified pipetting.

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Pichia EasyComp™ Transformation Kit (Invitrogen™)

The Pichia EasyComp™ Transformation Kit is a unique kit designed for the rapid preparation and transformation of competent Pichia pastoris cells. The Pichia EasyComp™ Transformation Kit offers several advantages over commonly used transformation procedures such as spheroplast formation:

• Competent cells can be frozen for future use, minimizing waste
• Competent cells can be prepared in less than 30 minutes
• Competent cell preparation and transformation solutions are optimized for use with Pichia pastoris are provided ready to use and are tested to ensure your results

imMedia™ Growth Medium, agar, ampicillin, X-gal/IPTG (Invitrogen™)

imMedia Growth Medium is pre-mixed, pre-sterilized E. coli growth medium that can be prepared in just five minutes without autoclaving. imMedia Growth Medium contains everything you need to prepare low-salt LB agar plates. You'll save hours of medium preparation time because there is:

• No weighing or mixing of media components
• No autoclaving
• No waiting for media to cool before adding antibiotics

Fast medium preparation
imMedia Growth Medium is fast food for E. coli. Simply mix with water in a clean flask, heat in a microwave oven, and in less than 5 minutes your medium is prepared.

The ultimate convenience
For your convenience, imMedia Growth Medium is available for preparing liquid medium or agar plates, with or without X-gal/IPTG. You have a choice of two antibiotics: ampicillin and kanamycin. imMedia Growth Medium is packaged in ready-to-use individual pouches. Each pouch contains sufficient reagents for preparing 200 mL of liquid media or 8-10 standard 100 mm2 agar plates.

Quality results
Each type of imMedia Growth Medium is extensively tested to ensure E. coli growth, antibiotic activity, and sterility. imMedia "Blue" products (with X-gal/IPTG) are tested for efficient blue/white colony screening.

BG-11 Media, optimized for cyanobacteria (Gibco™)

Gibco® BG-11 Media is optimized for the growth and maintenance of select Cyanobacteria including Synechococcus elongates. The formulation comes ready-to-use at 1X to let you avoid laborious media preparation steps.

Each Gibco® BG-11 Media comes in the award winning Gibco® bottle designed for easier use in the biosafety cabinet, minimizing the risk of contamination, and helps you perform cell culture more consistently. Together, the superior packaging and quality, greater reliability, and improved consistency in Cyanobacteria culture, results in better overall efficiency and more robust data (Fig. 1, 2).

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

MagicMedia™ E. coli Expression Medium, powder (Invitrogen™)

MagicMedia E. coli Expression Medium is specifically designed to dramatically increase the yield of recombinant proteins in T7-regulated E. coli expression systems without monitoring culture OD (optical density) or adding inducer. The proprietary MagicMedia formulation enables E. coli growth to reach culture densities 3–10-fold higher than those achieved with traditional LB + IPTG methods, allowing you to:

• Achieve 3–10 times higher protein yields (Figure 1) with much less hands-on time
• Eliminate time-consuming OD monitoring steps—simply inoculate, grow the culture overnight, and harvest (Figure 2)
• Reduce culture volume to simplify handling and scale-up processes
• Ideal for simultaneous expression of multiple clones for high-throughput applications

MagicMedia E. coli Expression Medium, powder, consists of a premixed powder packaged in a convenient foil pouch, a proprietary component A (in foil pouch powder format), and component B (in buffer liquid format).

MagicMedia E. coli Expression Medium is also available in a ready-to-use, pre-sterilized liquid format (Cat. No. K6803).

YPD Broth (Gibco™)

Gibco® YPD Broth is a ready-to-use liquid growth media used for yeast culture. Our filter-sterilized Gibco® YPD Broth can be used for nearly all research applications that require a liquid yeast culturing step such as protein expression, cloning, and expansion.
• Gibco® microbiological media
• Convenient ready-to-use liquid format
• 1 L size available

YPD Broth Recipe
YPD Broth is a commonly used yeast growth media for maintenance and propagation of Pichia pastoris (P. pastoris) and Saccharomyces cerevisae (S. cerevisae) yeast strains.

Formulation per One Liter:
10 g   Yeast extract
20 g   Bacto peptone
20 g   Dextrose (glucose)

Ready-to-Use in Optimized Gibco® Media Bottles
Each bottle of Gibco® YPD Broth is filter-sterilized and ready-to-use in ergonomic Gibco® media bottles with angled openings for simplified pipetting.

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Yeast Nitrogen Base (Invitrogen™)

Yeast nitrogen base (YNB) is a base medium for preparation of minimal and synthetic defined yeast media. YNB contains ammonium sulfate but does not contain amino acids. Both bulk and convenient single-use pouches are available.

S.O.C. Medium (Invitrogen™)

S.O.C. Medium is used in the final step of bacterial cell transformation to obtain maximal transformation efficiency of E. coli. S.O.C. Medium is supplied as 10 x 10 mL bottles of liquid medium with the following composition: 2% tryptone, 0.5% yeast extract, 10 mM NaCl, 2.5 mM KCl, 10 mM MgCl2, 10 mM MgSO4, and 20 mM glucose.

Quality testing
Each lot of S.O.C. Medium is tested to ensure conformance with the most current approved product specifications. This currently consists of tests for pH, osmolality, sterility, and transformation efficiency using TOP10 E. coli.

Select Agar™, powder (Invitrogen™)

An agent used for making solid microbiological media.

imMedia™ Growth Medium, liquid, kanamycin (Invitrogen™)

imMedia Growth Medium is pre-mixed, pre-sterilized E. coli growth medium that can be prepared in just five minutes without autoclaving. imMedia Growth Medium contains everything you need to prepare low-salt LB liquid medium. You'll save hours of medium preparation time because there is:

• No weighing or mixing of media components
• No autoclaving
• No waiting for media to cool before adding antibiotics

Fast medium preparation
imMedia Growth Medium is fast food for E. coli. Simply mix with water in a clean flask, heat in a microwave oven, and in less than 5 minutes your medium is prepared.

The ultimate convenience
For your convenience, imMedia Growth Medium is available for preparing liquid medium or agar plates, with or without X-gal/IPTG. You have a choice of two antibiotics: ampicillin and kanamycin. imMedia Growth Medium is packaged in ready-to-use individual pouches. Each pouch contains sufficient reagents for preparing 200 mL of liquid media or 8-10 standard 100 mm2 agar plates.

Quality results
Each type of imMedia Growth Medium is extensively tested to ensure E. coli growth, antibiotic activity, and sterility. imMedia "Blue" products (with X-gal/IPTG) are tested for efficient blue/white colony screening.

LB Agar, powder (Lennox L agar) (Invitrogen™)

Formulation per one liter:
10 g SELECT Peptone 140
5 g SELECT Yeast Extract
5 g sodium chloride
12 g SELECT Agar

Luria Broth Base (Miller's LB Broth Base)™, powder (Invitrogen™)

Formulation per one liter:
10 g SELECT Peptone 140
5 g SELECT Yeast Extract
10 g sodium chloride