Shop All Transfection Reagents

Lipofectamine™ LTX Reagent with PLUS™ Reagent (Invitrogen™)

Lipofectamine® LTX Reagent is a plasmid transfection reagent that offers the right balance of potency and gentleness for your cells, resulting in the highest possible transfection efficiencies and viabilities. Lipofectamine® LTX Reagent is an advanced solution for gene expression in both common and challenging cell types. No other plasmid DNA-specific transfection reagent can match the efficiency, convenience, and gentleness of Lipofectamine® LTX Reagent for transfection of primary, challenging, and sensitive cell lines. Lipofectamine® LTX Reagent is synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cell lines.

Lipofectamine® LTX Reagent offers:

• Superior delivery—transfect a broad range of cell types, including primary, challenging, and sensitive cells, with high efficiency
• Superior performance—combine high transfection efficiency with maximal (>90%) cell viability
• Optimized protocols—rapid, simple protocols provided for most cell lines
• A highly effective reagent to generate stable cell lines

Transfect primary cells efficiently
Primary cells are often difficult to transfect. Lipofectamine® LTX Reagent, when combined with PLUS™ Reagent, provides the highest level of transfection in primary cells of any lipid- or polymer-based reagent. Many primary cell types have been successfully transfected with Lipofectamine® LTX Reagent, including primary neural progenitor cells.

Superior delivery into challenging cells
When it comes to achieving high levels of protein expression in RAW267.3 macrophages, Lipofectamine® LTX Reagent outperforms competing transfection reagents (see figure). Achieving high-level transgene expression in cells that are traditionally refractory to transfection allows you to obtain results that were previously unattainable.

Broad compatibility with cell lines
In addition to being excellent for transfecting primary and challenging cells, Lipofectamine® LTX Reagent provides you with the most versatile approach to transfecting established cells lines, including those that are sensitive to other transfection reagents. Use our Transfection Selection Tool to help you select the best transfection conditions for the cells with which you work.

Stable transfection
Lipofectamine® LTX strikes the right balance between potency and gentleness, giving you the highest possible percentage of successfully transfected cells. This makes Lipofectamine® LTX Reagent the quickest and easiest reagent for establishing stable transfectants from challenging or refractory cell types.

Simple, rapid, serum-compatible protocol
Lipofectamine® LTX Reagent offers a streamlined protocol—no need to remove transfection complexes or change/add medium following transfection. A simple optimization protocol helps you get the best results quickly, so you'll spend less hands-on time, and get faster results.

siPORT™ Amine Transfection Agent (Invitrogen™)

A polyamine-based Ambion® siRNA transfection agent that can be used to transfect cells during subculturing-—saving a full day of valuable time—without increasing toxicity. It is supplied in one tube containing 0.4 mL. A successful siRNA experiment requires the appropriate transfection agent for your cells, and one that is optimized for siRNA delivery. The efficiency with which different transfection reagents deliver nucleic acids can vary dramatically with the type of nucleic acid being transfected (RNA vs. DNA, single-stranded vs. double-stranded) and the cells being used.

Accessory Products:
siPORT™ NeoFX™ Transfection Agent (SKU#s AM4511 and AM4510), a cationic and neutral lipid mixture, is also available. For testing which transfection agent is best with your system, the Silencer® siRNA Transfection II Kit (SKU# AM1631) is recommended. It contains the two different transfection agents along with controls for transfection optimization.

293fectin™ Transfection Reagent (Gibco™)

293fectin™ Transfection Reagent is a proprietary, cationic lipid-based formulation for transfecting DNA into eukaryotic cells. This reagent is optimized for transfecting suspension 293 human embryonic kidney cells (e.g., FreeStyle™ 293-F cells, Cat. No. R790-07) in defined, serum-free FreeStyle™ 293 Expression Medium (Cat. No. 12338-018), and is intended for use with the FreeStyle™ 293 Expression System (Cat. No. K9000-01). 293fectin™ Transfection Reagent provides the following advantages:

• Demonstrates high transfection efficiency in suspension 293 cells and is also suitable for transfecting adherent 293 cells
• Suspension FreeStyle™ 293-F cells may be transfected in FreeStyle™ 293 Expression Medium; no medium change is required
• Add 293fectin™ reagent complexes directly to cells in culture medium
• No need to remove complexes or change or add medium following transfection

siPORT™ Amine Transfection Agent with manual (Invitrogen™)

A polyamine-based Ambion® siRNA transfection agent that can be used to transfect cells during subculturing-—saving a full day of valuable time—without increasing toxicity. It is supplied in one tube containing 0.4 mL. A successful siRNA experiment requires the appropriate transfection agent for your cells, and one that is optimized for siRNA delivery. The efficiency with which different transfection reagents deliver nucleic acids can vary dramatically with the type of nucleic acid being transfected (RNA vs. DNA, single-stranded vs. double-stranded) and the cells being used.

Accessory Products:
siPORT™ NeoFX™ Transfection Agent (SKU#s AM4511 and AM4510), a cationic and neutral lipid mixture, is also available. For testing which transfection agent is best with your system, the Silencer® siRNA Transfection II Kit (SKU# AM1631) is recommended. It contains the two different transfection agents along with controls for transfection optimization.

Lipofectamine™ Stem Transfection Reagent (Invitrogen™)

Lipofectamine Stem Transfection Reagent is optimized to achieve maximum efficiency with minimal early differentiation in a wide range of stem cells. It can co-deliver DNA, RNA, and Cas9 ribonucleoprotein (RNP) complexes. Lipofectamine Stem reagent is compatible with a variety of media systems, including feeder-free, helping support and simplify your stem cell culture workflow. Researchers can achieve up to 80% or better transfection efficiency in pluripotent stem cells (PSCs) and neural stem cells (NSCs), and up to 45% in mesenchymal stem cells (MSCs).

Transfecting stem cells without inhibiting cell viability and cell growth can be challenging due to the sensitivity of these cells. Lipofectamine Stem is a low toxicity reagent, requiring low amounts of nucleic acid, allowing your stem cells to stay healthy and continue proliferating without inducing differentiation. Lipofectamine Stem reagent is:

Superior in performance—up to 3-fold higher efficiency across iPSCs, hESCs, NSCs, and MSCs compared to other reagents
Versatile—one reagent for DNA, RNA, Cas9 protein, and co-transfection
Gentle on cells—maintains cell proliferation without inducing differentiation
Flexible—transfects cells in adherence or suspension—an alternative to electroporation

Achieve high efficiency gene editing in stem cells
Gene editing applications typically require the ability to co-deliver DNA, RNA, and/or protein into cells. Lipofectamine Stem Transfection Reagent is designed to co-deliver CRISPR/Cas9 complexes into human stem cells for high efficiency gene editing. This one reagent can be used to transfect large DNA plasmids such as all-in-one CRISPR/Cas9 vectors and co-deliver a range of RNAs, such as large Cas9 mRNA with tracr/crRNA complexes or single guide (sg) RNA, and protein such as Cas9 protein combined with tracr/crRNA or sgRNA.

A low-toxicity alternative to electroporation
Transfecting stem cells via electroporation methods can be challenging and cumbersome. Electroporation protocols recommend single cell suspension culture to ensure maximum efficiency and minimal toxicity. Using the reverse transfection protocol, the Lipofectamine Stem Transfection Reagent proves to be an effective and less toxic alternative to electroporation for stem cells. It requires low amounts of nucleic acid to achieve maximum transfection efficiency, on par with electroporation, while supporting continued proliferation without inducing differentiation.

TurboFect™ Transfection Reagent (Thermo Scientific™)

Thermo Scientific TurboFect Transfection Reagent is a highly efficient, easy-to-use, non-immunogenic transfection reagent for delivery of DNA plasmids and expression vectors into eukaryotic cells.

Features of TurboFect Transfection Reagent:

• High transfection efficiency of a wide variety of cell types
• No optimization required for most cell lines
• Excellent transfection efficiency in the presence or absence of serum
• Minimal cytotoxicity
• Ready-to-use—no need to reconstitute, dilute or manipulate

Applications of TurboFect Transfection Reagent :
• Stable and transient transfection or co-transfection of DNA or RNA
• Experiments with adherent or suspension cells, differentiated or undifferentiated cells
• Gene expression studies and assays involving GFP or luciferase reporters

TurboFect Transfection Reagent is a sterile solution of a proprietary cationic polymer. TurboFect Reagent interacts with DNA to form small, stable, highly diffusible complexes which are readily endocytosed. The reagent creates a proton-sponge effect that buffers endosomal pH by provoking massive proton accumulation and passive chloride influx. As a result, rapid osmotic swelling of the endocytosed DNA-complex causes it to rupture in the cytosol, allowing translocation of DNA to the nucleus. TurboFect Reagent is ideal for transfection of a variety of cells, including primary and difficult-to-transfect cells.

PEI-Transferrinfection Kit (Invitrogen™)

Transfection of DNA into eukaryotic cells is a common method to study biological mechanisms. A major goal is the efficient and specific delivery of genes into the desired target cells. Although a wide panel of techniques and vectors (viral and nonviral) have been developed that work with variable efficiency, most vectors lack a target cell specificity. Nonviral vectors are attractive because of their ease of manipulation, safety and high flexibility in the size of the delivered transgene. The Transferrinfection is a high-efficiency nucleic acid delivery system based on transferrin receptor-mediated endocytosis to carry DNA into cells. Furthermore it was shown that the cationic polymer polyethylenimine (PEI) mediates efficient gene transfer into a variety of cells. In the Polyethylenimine-Transferrinfection system the gene transfer efficiency of PEI/DNA complexes are combined with the specific mechanism of receptor-mediated endocytosis via Transferrin receptor.

The method results in a 30 - 1000-fold enhanced transfection efficiency depending on the cell line. It is an extremely gentle DNA transfection method whichemploys physiological uptake mechanisms of the cell. Transfection efficacy depends on the cell type, the level of surface transferrin receptor expression. Very high transfection rates have been shown for the tested tumor cell lines B16F10 melanoma, Neuro 2A neuroblastoma and a variety of primary human melanoma cell lines. In other established cell lines, such as HeLa, CHO, Jurkat, K562, HepG2 and COS the PEI-Transferrinfection works with high efficiences, excellent reproducibility and with the advantage of being an extremely gentle procedure.

Reactivity/Species
Human

Suitable Sample Types
Suspension cultures, Adherent cells

Reported Application
Cell Culture

PLUS™ Reagent (Invitrogen™)

PLUS™ Reagent is used in conjunction with various transfection reagents, such as Lipofectamine™ Reagent, to improve transfection efficiency in adherent cell lines.

Using PLUS™ Reagent
Some customers find that when using PLUS™ Reagent, less optimization of DNA concentration is needed to achieve high transfection performance. The addition of PLUS™ Reagent may also reduce the quantities of DNA and lipid required by approximately one-half compared to lipid alone. PLUS™ Reagent was specifically designed to expand the array of transfectable cell types. When used with Lipofectamine™ Reagent, PLUS™ Reagent has been demonstrated to improve the transfection of BHK-21, NIH 3T3, and other cell lines.

ExpiFectamine™ CHO Transfection Kit (Gibco™)

The ExpiFectamine™ CHO Transfection Kit is a core component of the ExpiCHO™ Expression System Kit (Cat. No. A29133). It is designed for transient transfection of high-density cultures of Chinese hamster ovary (CHO) cells. The high-efficiency, cationic transfection reagent, coupled with an optimized transfection enhancer and culture feed, will power the highest possible level of protein expression from ExpiCHO-S™ Cells (Cat. No. A29127) cultured in ExpiCHO™ Expression Medium (Cat. No. A2910001).

The ExpiFectamine CHO Transfection Kit:
• Achieves greater than 90% transfection efficiency of high-density suspension CHO cell culture, with matching transfection enhancer and culture feed to boost transfection performance and protein expression
• Requires one-third to one-half less plasmid DNA than other transfection reagents while achieving superior expression performance
• Enables options for three transient expression protocols for maximal work flexibility while still fitting into the same overall workflow used by other transient expression systems, such as FreeStyle™ MAX CHO and Expi293™
• Delivers robust and reproducible transfection results, giving you greater confidence in your results
• Demonstrates linear scalability of transfections from culture volumes of <1 mL to >10 L

The ExpiFectamine CHO Transfection Kit is specifically designed to work with ExpiCHO-S Cells in the ExpiCHO™ Expression System Kit. The transfection kit supplies the cationic ExpiFectamine™ CHO Transfection Reagent, ExpiFectamine™ CHO Transfection Enhancer, and ExpiCHO Feed.

Lipofectamine™ CRISPRMAX™ Cas9 Transfection Reagent (Invitrogen™)

Lipofectamine CRISPRMAX Cas9 Transfection Reagent is the first optimized lipid nanoparticle transfection reagent for CRISPR-Cas9 protein delivery. It is a high-throughput-friendly, cost-effective alternative to electroporation.

Working together with our TrueCut Cas9 Protein v2 and TrueGuide Synthetic gRNA, it provides:
• Demonstrated cleavage efficiency—tested in over 20 cell types including iPSC, mESC, N2A, CHO, A549, HCT116, HeLa, HEK293, and several others
• Low cell toxicity—less cells needed to initiate your experiment
• High throughput friendly—an ideal delivery solution for 96-well format
• Cost savings—whether cost-per-reaction or initial investment

Achieve high transfection efficiency in novel genome editing applications
Lipofectamine CRISPRMAX transfection reagent increases the likelihood of successful cleavage and recombination, especially when combined with our TrueCut Cas9 Protein v2 and TrueGuide Synthetic gRNA. This ultimately maximizes the efficiency of performing genetic modifications.

Improve experimental outcomes using TrueCut Cas9 Protein v2
Unlike CRISPR plasmid or Cas9 mRNA, using Cas9 protein provides superior cleavage efficiency in primary cells and stem cells. It eliminates the need for transcription or translation of the payload, removes the risk of genomic integration, and is cell cycle independent.

FreeStyle™ MAX Reagent (Gibco™)

FreeStyle™ MAX Reagent is a proprietary, animal origin–free formulation for transfecting plasmid DNA into eukaryotic cells that can easily be scaled up to produce large amounts of recombinant proteins. FreeStyle™ MAX Reagent allows the highest expression levels and transfection rates with lowest cytotoxicity in bio-production applications, and is specifically formulated for use with:

• FreeStyle™ 293-F Cells (suspension human embryonal kidney cells, Cat. No. R790-07) in serum-free FreeStyle™ 293 Expression Medium (Cat. No. 12338-018)
• FreeStyle™ CHO-S Cells (suspension Chinese Hamster Ovary cells, Cat. No. R800-07) in serum-free FreeStyle™ CHO Expression Medium (Cat. No. 12651-014)
• DG44 Cells (DHFR suspension CHO cells) in CD DG44 Medium with 8 mM L-glutamine and 18 mL/L 10% Pluronic® F-68 (Cat. No. A11000-01)

Product use
FreeStyle™ MAX Reagent is intended for use with the FreeStyle™ MAX 293 Expression System (Cat. No. K9000-10) and the FreeStyle™ MAX CHO Expression System (Cat. No. K9000-20).

Lipofectamine™ MessengerMAX™ Transfection Reagent (Invitrogen™)

Lipofectamine® MessengerMAX mRNA Transfection Reagent delivers amazing transfection efficiency in neurons and a broad-spectrum of primary cells, enabling improved application outcomes and more biologically relevant research. That's because our novel lipid nanoparticle technology is optimized to deliver the highest amount of mRNA possible without the nuclear entry step that is required with DNA.

Successfully transfect more predictive cell models with a reagent that provides:
• Amazing transfection efficiency in neurons and primary cell types
• Faster protein expression with no risk of genomic integration
• Up to 10x higher cleavage using mRNA CRISPRs

Enable high transfection efficiency in novel genome editing applications
Lipofectamine® MessengerMAX™ reagent helps increase the likelihood of successful cleavage and recombination with GeneArt® CRISPR Nuclease mRNA through highly efficient transfection, ultimately maximizing the efficiency of genetic modifications and simplifying the downstream processes.

Efficiently transfect difficult-to-transfect cells
Lipofectamine® MessengerMAX™ reagent is designed to transfect a higher amount of mRNA into neurons and a broad spectrum of difficult-to-transfect primary cells. This eliminates the need for electroporation or viruses, and provides a > 2-fold improvement in transfection efficiency compared to other lipid based reagents.

Leverage advantages of mRNA transfection
Transfection of mRNA does not require nuclear uptake, which results in faster protein expression than DNA transfection since translation of mRNA occurs in the cytoplasm. Also, mRNA has no risk of genomic integration and transfection efficiency is cell cycle independent.

Lipofectamine™ Transfection Reagent (Invitrogen™)

Lipofectamine® Transfection Reagent is one of our first-generation, cationic-lipid transfection reagents formulated for the transfection of DNA into eukaryotic cells. Lipofectamine® Transfection Reagent is a trusted, value reagent with many years of cited use in thousands of publications and with many cell lines.

With Lipofectamine® Transfection Reagent, you'll get:

• A reagent proven to work in high-throughput applications
• A reliable reagent for establishing stable cell lines
• A reagent that works well with PLUS™ Reagent for higher protein expression

A proven technology
Our Lipofectamine®-brand reagents have been recognized as the most-cited transfection reagent family, with tens of thousands of citations since launch in 1993. Lipofectamine® Transfection Reagent is no exception as a trusted reagent for a wide range of cell lines. When used in combination with PLUS™ Reagent, Lipofectamine® Transfection Reagent has been shown to work well with cells such as BHK-21, NIH 3T3, COS-1, fibroblasts, keratinocytes, HT-29, MRC-5, and SK-BR3.

A "value" alternative for plasmid DNA
With such a legacy comes a commitment to providing the best reagents for all scientists and labs, regardless of their funding capabilities. Lipofectamine® Transfection Reagent is a value alternative for transfection of plasmid DNA into eukaryotic cells with a simplified protocol. For optimal results in a wider range of plasmid DNA transfection conditions, we recommend Lipofectamine® LTX Reagent, due to its optimal balance of potency & low-cytotoxicity. Lipofectamine® 2000 Transfection Reagent is recommended if you are transfecting a broad range of cell lines or want to transfect RNA.

TurboFect in vivo Transfection Reagent (Thermo Scientific™)

Thermo Scientific TurboFect in vivo Transfection Reagent is highly effective for DNA plasmid delivery in vivo, while not inducing an inflammatory response. TurboFect in vivo has been assessed for plasmid DNA delivery in both mice and rats and is suitable for DNA administration via various routes, including intravenous, intraperitoneal, and intratumoral injection. The amount of DNA and suggested injection volume will depend on the experimental animal and the route of administration as well as the targeted tissue or organ and the expression vector.

TurboFect in vivo is a sterile solution of a cationic polymer in water. The polymer forms compact, stable, positively charged complexes with DNA. These complexes protect DNA from degradation and facilitate gene delivery in vivo.

Highlights

• No detectable inflammatory response
• Successful plasmid delivery via multiple routes of administration
• Functionally tested in mice and rats

Lipofectamine™ 3000 Transfection Reagent (Invitrogen™)

Lipofectamine® 3000 Transfection Reagent leverages our most advanced lipid nanoparticle technology to provide superior transfection performance with improved application outcomes and reproducible results. This reagent delivers superior transfection efficiency and improved cell viability for the widest range of hard-to-transfect and common cells (e.g., HEK293, HeLa).

Successfully transfect the widest variety of biologically relevant cell types with a reagent that offers:

• Superior performance—our highest efficiency reagent for difficult-to-transfect cells
• Improved cell viability—gentle on your cells, with low toxicity
• Versatile—one reagent for DNA, RNA, and co-transfection

Efficiently Transfect Difficult-to-Transfect Cells
Lipofectamine® 3000 reagent is designed to efficiently transfect difficult-to-transfect cells, yielding superior transfection performance across the broadest array of cell types. Successfully transfected cell types include:



Fibroblast (3T3, COS-7)Myoblast (C2C12, L6 CRL-1458)
Hepatocyte (HepG2, HuH7)Erythroleukemic cell (K562)
Breast carcinoma (MCF7, Hs578T)Prostate carcinoma (LNCap)
Lung carcinoma (A549, NCI-H460)Osteosarcoma (U-2 OS, Saos-2)
Colon carcinoma (Caco2, SW480)Pancreatic carcinoma (PANC-1)
Skin melanoma (SK-MEL-28) 


View performance data for 60+ cell lines plus comparison to Lipofectamine 2000 >

Transfect Common Cells Efficiently and Cost-Effectively
For common cell types, Lipofectamine® 3000 reagent is more cost-effective than other reagents due to its high concentration and low usage amount. The 1.5 mL size provides up to 1500 transfection reactions (in 24-well plates).

Achieve Superior Transfection Efficiency While Maintaining Low Toxicity
Lipofectamine® 3000 reagent maintains a high transfection efficiency within a robust dynamic range of lipid doses for quick and easy optimization. A low toxicity lipid dose (0.75 µL for 24-well plates) is suggested for applications requiring minimal disruption of the cells.

Enable High Transfection Efficiency in Novel Genome Editing Applications
Lipofectamine® 3000 reagent is suitable for novel genome editing applications. It increases the likelihood of successful cleavage and recombination with TALENs or CRISPRs through highly efficient transfection, ultimately maximizing the efficiency of genetic modifications and simplifying the downstream processes.