Shop All Transfection Reagents

LV-MAX™ Transfection Kit (Gibco™)

The Gibco LV- MAX Transfection Kit is an essential component of the LV-MAX Lentiviral Production System. It is formulated for the transient transfection of Gibco Viral Production Cells (high-density suspension-adapted human embryonic kidney (HEK) 293-derived cells). The included high-efficiency, cationic, lipid-based transfection reagent, lentiviral production supplement, and LV enhancer are designed to work together to produce the highest possible level of lentiviral particles from the Viral Production Cells when cultured in LV-MAX Production Medium.

• Designed specifically for transfection of high-density suspension cells to maximize lentiviral production
• Enables sustained, high-level production of high-density transiently-transfected cultures, achieving titer of up to 1 x 108 TU/mL (unconcentrated LVVs-GFP)
• Reproducible and scalable transfection results, giving users greater confidence in their results
• Easy, robust culture and transfection protocols—fits into any transient production workflowsThe kit includes three key components required to optimize your lentiviral production. These are only available in the kit and are not sold separately.
• LV-MAX transfection reagent—a high-efficiency, cationic, lipid-based transfection reagent uniquely designed for co-transfecting multiple plasmids into high-density Viral Production Cell cultures with high transfection efficiency and low toxicity
• LV-MAX supplement—an optimized, chemically defined, serum-free, protein-free, animal origin-free formulation designed to suppress cell growth during transfection and increase lentiviral vector production without compromising the cell viability and interfering with the lentiviral production process
• LV-MAX enhancer—a proprietary, animal origin-free formulation designed to boost cell lentiviral vector production

Cellfectin™ II Reagent (Gibco™)

Cellfectin® II Reagent is a cationic-lipid formulation designed for optimal transfection of insect cells. Cellfectin® II is an improved version of the original Cellfectin® Reagent. In addition to providing the same great performance as Cellfectin®, Cellfectin® II Reagent is manufactured to ensure lot-to-lot consistency, and is optimized to be used in a faster protocol. Transfection with Cellfectin® II Reagent leads to consistent and efficient transfection of Sf9, Sf21, and High Five™ cells when using BaculoDirect™ and InsectSelect™ Expression Systems. Cellfectin® II Reagent can also be used to transfect adherent or suspension mammalian cells in serum-containing or serum-free media.

PLUS™ Reagent (Invitrogen™)

PLUS™ Reagent is used in conjunction with various transfection reagents, such as Lipofectamine™ Reagent, to improve transfection efficiency in adherent cell lines.

Using PLUS™ Reagent
Some customers find that when using PLUS™ Reagent, less optimization of DNA concentration is needed to achieve high transfection performance. The addition of PLUS™ Reagent may also reduce the quantities of DNA and lipid required by approximately one-half compared to lipid alone. PLUS™ Reagent was specifically designed to expand the array of transfectable cell types. When used with Lipofectamine™ Reagent, PLUS™ Reagent has been demonstrated to improve the transfection of BHK-21, NIH 3T3, and other cell lines.

Lipofectamine™ LTX Reagent with PLUS™ Reagent (Invitrogen™)

Lipofectamine® LTX Reagent is a plasmid transfection reagent that offers the right balance of potency and gentleness for your cells, resulting in the highest possible transfection efficiencies and viabilities. Lipofectamine® LTX Reagent is an advanced solution for gene expression in both common and challenging cell types. No other plasmid DNA-specific transfection reagent can match the efficiency, convenience, and gentleness of Lipofectamine® LTX Reagent for transfection of primary, challenging, and sensitive cell lines. Lipofectamine® LTX Reagent is synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cell lines.

Lipofectamine® LTX Reagent offers:

• Superior delivery—transfect a broad range of cell types, including primary, challenging, and sensitive cells, with high efficiency
• Superior performance—combine high transfection efficiency with maximal (>90%) cell viability
• Optimized protocols—rapid, simple protocols provided for most cell lines
• A highly effective reagent to generate stable cell lines

Transfect primary cells efficiently
Primary cells are often difficult to transfect. Lipofectamine® LTX Reagent, when combined with PLUS™ Reagent, provides the highest level of transfection in primary cells of any lipid- or polymer-based reagent. Many primary cell types have been successfully transfected with Lipofectamine® LTX Reagent, including primary neural progenitor cells.

Superior delivery into challenging cells
When it comes to achieving high levels of protein expression in RAW267.3 macrophages, Lipofectamine® LTX Reagent outperforms competing transfection reagents (see figure). Achieving high-level transgene expression in cells that are traditionally refractory to transfection allows you to obtain results that were previously unattainable.

Broad compatibility with cell lines
In addition to being excellent for transfecting primary and challenging cells, Lipofectamine® LTX Reagent provides you with the most versatile approach to transfecting established cells lines, including those that are sensitive to other transfection reagents. Use our Transfection Selection Tool to help you select the best transfection conditions for the cells with which you work.

Stable transfection
Lipofectamine® LTX strikes the right balance between potency and gentleness, giving you the highest possible percentage of successfully transfected cells. This makes Lipofectamine® LTX Reagent the quickest and easiest reagent for establishing stable transfectants from challenging or refractory cell types.

Simple, rapid, serum-compatible protocol
Lipofectamine® LTX Reagent offers a streamlined protocol—no need to remove transfection complexes or change/add medium following transfection. A simple optimization protocol helps you get the best results quickly, so you'll spend less hands-on time, and get faster results.

ExpiFectamine™ CHO Transfection Kit (Gibco™)

The ExpiFectamine™ CHO Transfection Kit is a core component of the ExpiCHO™ Expression System Kit (Cat. No. A29133). It is designed for transient transfection of high-density cultures of Chinese hamster ovary (CHO) cells. The high-efficiency, cationic transfection reagent, coupled with an optimized transfection enhancer and culture feed, will power the highest possible level of protein expression from ExpiCHO-S™ Cells (Cat. No. A29127) cultured in ExpiCHO™ Expression Medium (Cat. No. A2910001).

The ExpiFectamine CHO Transfection Kit:
• Achieves greater than 90% transfection efficiency of high-density suspension CHO cell culture, with matching transfection enhancer and culture feed to boost transfection performance and protein expression
• Requires one-third to one-half less plasmid DNA than other transfection reagents while achieving superior expression performance
• Enables options for three transient expression protocols for maximal work flexibility while still fitting into the same overall workflow used by other transient expression systems, such as FreeStyle™ MAX CHO and Expi293™
• Delivers robust and reproducible transfection results, giving you greater confidence in your results
• Demonstrates linear scalability of transfections from culture volumes of <1 mL to >10 L

The ExpiFectamine CHO Transfection Kit is specifically designed to work with ExpiCHO-S Cells in the ExpiCHO™ Expression System Kit. The transfection kit supplies the cationic ExpiFectamine™ CHO Transfection Reagent, ExpiFectamine™ CHO Transfection Enhancer, and ExpiCHO Feed.

Lipofectamine™ RNAiMAX Transfection Reagent (Invitrogen™)

Lipofectamine® RNAiMAX Transfection Reagent provides the highest transfection efficiencies on the widest variety of cell types for siRNA-mediated gene knockdown experiments. Lipofectamine® RNAiMAX is a proprietary RNAi-specific cationic lipid formulation designed specifically for the delivery of siRNA and miRNA into all cell types.

With Lipofectamine® RNAiMAX Transfection Reagent you will get:

• Superior transfection efficiency requiring lower RNAi concentrations, leading to more effective gene knockdown with minimal nonspecific effects
• Easy optimization due to minimal cytotoxicity across a 10-fold concentration range of transfection reagent
• Superior transfection efficiencies for miRNA antagonists and mimics
• Compatibility with a broad range of cell types, providing the most versatile approach to all of your gene silencing experiments
• A simple and rapid protocol for consistent and reproducible results

High knockdown in a wide range of cells
Lipofectamine® RNAiMAX Transfection Reagent transfects a wide range of cell types (see figure). For gene silencing, Lipofectamine® RNAiMAX Transfection Reagent's high-efficiency transfections lead to the high levels of gene knockdown needed to achieve convincing results.

Simple, high-throughput–ready transfections
Simply mix Lipofectamine® RNAiMAX Transfection Reagent with siRNA, add to your cells, incubate, and measure gene knockdown. The simplicity and speed combined with high transfection efficiency make Lipofectamine® RNAiMAX Transfection Reagent ideal for high-throughput siRNA transfections. Transfection conditions can be easily established for automated or robotic systems used in such applications.

Find an optimized Lipofectamine® RNAiMAX transfection protocol for your cell line.

siPORT™ NeoFX™ Transfection Agent with manual (Invitrogen™)

This Ambion® lipid-based formulation can be used to efficiently transfect adherent cells with siRNA as they are subcultured—without increased cytotoxicity. It is provided in one tube containing 1 mL.

• Versatile—works with a broad range of cell lines
• Reproducible—produces consistent results, lot-to-lot, plate-to-plate, and well-to-well
• Efficient—performs with high siRNA transfection efficiency, allowing use of low siRNA concentrations to minimize nonspecific effects
• Fast—reverse-transfects cells as they are plated, saving a day

Gene silencing experiments often call for critical, but time-consuming optimization experiments. siPORT™ NeoFX Transfection Agent refines siRNA transfection protocols resulting in less optimization. From start to finish, successful gene silencing experiments can be completed in as early as 24 hours. This streamlined protocol can be adapted to a wide range of cells and experimental designs, including high-throughput applications.

Reverse-Transfect Cells as They are Plated, Saving a Day
Just add siPORT™ NeoFX to your diluted siRNA, incubate to form transfection complexes, add the complexes to the culture wells, and overlay with cells. This new streamlined protocol, named "neofection", will save researchers a full day of valuable time when compared to traditional plated transfection procedures. The transfection complexes are active and stable even in the presence of serum, so there is no need to remove or replace media following transfection.

Optimized for Lower siRNA Concentrations and High Reproducibility
Recent reports indicate that siRNA concentrations of 100 nM or higher can lead to nonspecific changes in gene expression (off-target effects) in mammalian cultured cells. Reducing the amount of siRNA used for transfections to 1–20 nM minimizes these nonspecific effects, while still providing extensive silencing of the target gene. siPORT™ NeoFX Transfection Agent efficiently transfects low concentrations of siRNA. Furthermore, this agent delivers high performance among multiple wells for replicate samples and between different lots of the transfection agent.

Compatible with a Broad Range of Cell Lines
The agent has been used successfully to deliver siRNA into many cell types, including MCF-7 (human breast adenocarcinoma), HT-29 (human colorectal adenocarcinoma), HeLa-S3 (human cervix adenocarcinoma), HeLa (human cervix adenocarcinoma), HepG2 (human hepatocellular carcinoma), BJ (human foreskin fibroblast), A549 (human lung carcinoma), UMR106 (rat osteosarcoma), SKOv3 (human ovarian carcinoma), and SKNAS (human bone marrow neuroblastoma).

Accessory Products:
siPORT™ Amine Transfection Agent (SKU#s AM4502 and AM4503), a polyamine agent, is also available. For testing which transfection agent is best with your system, the Silencer® siRNA Transfection II Kit (SKU# AM1631) is recommended. It contains the two different transfection agents along with controls for transfection optimization.

TurboFect Transfection Reagent (Thermo Scientific™)

Thermo Scientific TurboFect Transfection Reagent is recommended for efficient transfection of plasmid DNA. Distinct from lipid-based formulas, TurboFect is a sterile solution of a proprietary cationic polymer in water. The polymer forms positively-charged complexes with DNA that are both compact and stable. These complexes protect DNA from degradation and facilitate efficient plasmid delivery into eukaryotic cells.

Highlights

• Excellent transfection efficiency in the presence or absence of serum
• Ready-to-use reagent – no need to reconstitute, dilute, or manipulate
• Simple protocol

High transfection efficiency of plasmid DNA into a wide variety of cell types
• Adherent and suspension cells
• Differentiated and undifferentiated cells

Review our experimental considerations to improve your plasmid transfection results.

Lipofectamine™ MessengerMAX™ Transfection Reagent (Invitrogen™)

Lipofectamine® MessengerMAX mRNA Transfection Reagent delivers amazing transfection efficiency in neurons and a broad-spectrum of primary cells, enabling improved application outcomes and more biologically relevant research. That's because our novel lipid nanoparticle technology is optimized to deliver the highest amount of mRNA possible without the nuclear entry step that is required with DNA.

Successfully transfect more predictive cell models with a reagent that provides:
• Amazing transfection efficiency in neurons and primary cell types
• Faster protein expression with no risk of genomic integration
• Up to 10x higher cleavage using mRNA CRISPRs

Enable high transfection efficiency in novel genome editing applications
Lipofectamine® MessengerMAX™ reagent helps increase the likelihood of successful cleavage and recombination with GeneArt® CRISPR Nuclease mRNA through highly efficient transfection, ultimately maximizing the efficiency of genetic modifications and simplifying the downstream processes.

Efficiently transfect difficult-to-transfect cells
Lipofectamine® MessengerMAX™ reagent is designed to transfect a higher amount of mRNA into neurons and a broad spectrum of difficult-to-transfect primary cells. This eliminates the need for electroporation or viruses, and provides a > 2-fold improvement in transfection efficiency compared to other lipid based reagents.

Leverage advantages of mRNA transfection
Transfection of mRNA does not require nuclear uptake, which results in faster protein expression than DNA transfection since translation of mRNA occurs in the cytoplasm. Also, mRNA has no risk of genomic integration and transfection efficiency is cell cycle independent.

LV-MAX™ Lentiviral Production System (Gibco™)

The Gibco LV-MAX Lentiviral Production System is the first optimized system that provides a scalable and high-yield lentiviral vector production platform. It is based on a suspension, high-density culture of HEK 293-derived Viral Production Cells adapted to a special chemically defined, serum-free and protein-free LV-MAX Production Medium. Optimal viral titer yield is mediated by our advanced lipid nanoparticle transfection reagent in combination with a novel lentiviral-specific enhancer and supplement. All components work synergistically to generate superior and functional lentiviral particles under serum-free conditions compared to conventional polyethylenimine (PEI).

The LV-MAX Lentiviral Production System provides:
• High titer—greater than 1 x 108 TU/mL (unconcentrated LVVs-GFP)
• Scalable suspension system—from 96 deep-well plate to 2-L bioreactor
• Serum-free—eliminating animal-origin components and associated risks
• Easy, robust culture and transfection protocols—fits into current transient production workflows

The components included in the LV-MAX Lentiviral Production System are: two vials of frozen Viral Production Cells, one liter of LV-MAX Production Medium, and one LV-MAX Transfection Kit sufficient to produce 0.3 L of lentivirus. All components of the LV-MAX Lentiviral Production System are also available for purchase separately. Also required, but not included in this kit, is Gibco Opti-MEM I Reduced Serum Medium (Cat. No. 31985062).

Lipofectamine™ 2000 Transfection Reagent (Invitrogen™)

Lipofectamine® 2000 Transfection Reagent is a versatile transfection reagent that has been shown to effectively transfect the widest variety of adherent and suspension cell lines. Researchers use Lipofectamine® 2000 Reagent for siRNA- and shRNA-based gene knockdown experiments, as well as for gene expression studies.

With Lipofectamine® 2000 Transfection Reagent, you'll get:

• Exceptional transfection efficiency in the broadest range of cell lines and the highest levels of recombinant protein expression (see table)
• Superior performance for co-transfection of siRNA and plasmid DNA
• Proven efficacy in the presence of serum—eliminates the need to change media following transfection
• Reliable performance for high-throughput applications
• The best choice for establishing stable cell lines

A high-performance transfection reagent for gene expression and gene silencing
Lipofectamine® 2000 Transfection Reagent works effectively with all common cell lines as well as with many challenging ones, and can be used in media with or without serum. For gene silencing, Lipofectamine® 2000 Transfection Reagent's high-efficiency transfections provide the high levels of gene knockdown needed to produce convincing results. Lipofectamine® 2000 Transfection Reagent is the number one choice for co-transfection, given its effectiveness for transfecting both siRNA and plasmid DNA. Lipofectamine® 2000 Transfection Reagent is easy to use—simply mix with nucleic acid and add to cell culture.

Ideal for high-throughput work
Simplicity and speed combined with high transfection efficiency make Lipofectamine® 2000 Transfection Reagent ideal for transient protein expression or high-throughput RNAi transfections. Transfection conditions can be easily established for automated or robotic systems used in such applications.

Invivofectamine™ 3.0 Reagent (Invitrogen™)

Invivofectamine® 3.0 Reagent is an animal origin-free lipid nanoparticle designed for high efficiency in vivo delivery of siRNA and miRNA to mouse liver cells following tail vein injection.

Using Invivofectamine® 3.0 Reagent
Invivofectamine® 3.0 Reagent is ideally suited for in vivo delivery of siRNA and miRNA duplexes. Invivofectamine® 3.0/RNA complexes are delivered using a low-volume, low-pressure method with no toxicity and no stress response in the animal. Invivofectamine® 3.0 can also be combined with BLOCK-iT™ fluorescent controls to track the biodistribution of the siRNA or miRNA.

DMRIE-C Transfection Reagent (Invitrogen™)

DMRIE-C Transfection Reagent is suitable for transfecting DNA and RNA into eukaryotic cells, and is particularly effective for transfecting suspension cells (e.g., Jurkat) and other lymphoid-derived cell lines. Refer to the Cell Lines database at www.invitrogen.com for a list of cell types successfully transfected. DMRIE-C can also be used for in vivo delivery of DNA. DMRIE-C is a 1:1 (M/M) liposome formulation of the cationic lipid DMRIE (1,2-dimyristyloxy-propyl-3-dimethyl-hydroxy ethyl ammonium bromide) and cholesterol in membrane-filtered water.

293fectin™ Transfection Reagent (Gibco™)

293fectin™ Transfection Reagent is a proprietary, cationic lipid-based formulation for transfecting DNA into eukaryotic cells. This reagent is optimized for transfecting suspension 293 human embryonic kidney cells (e.g., FreeStyle™ 293-F cells, Cat. No. R790-07) in defined, serum-free FreeStyle™ 293 Expression Medium (Cat. No. 12338-018), and is intended for use with the FreeStyle™ 293 Expression System (Cat. No. K9000-01). 293fectin™ Transfection Reagent provides the following advantages:

• Demonstrates high transfection efficiency in suspension 293 cells and is also suitable for transfecting adherent 293 cells
• Suspension FreeStyle™ 293-F cells may be transfected in FreeStyle™ 293 Expression Medium; no medium change is required
• Add 293fectin™ reagent complexes directly to cells in culture medium
• No need to remove complexes or change or add medium following transfection

Lipofectamine™ 2000 CD Transfection Reagent (Invitrogen™)

Lipofectamine® 2000 CD (Chemically Defined) Transfection Reagent is a proprietary animal origin-free formulation for transfecting nucleic acids into eukaryotic cells. Using Lipofectamine® 2000 CD reagent for transfection provides the following advantages:

• Highest transfection efficiency in many cell types and formats.
• The animal origin-free formulation ensures that mammalian cell culture and bioproduction processes are free of animal-derived materials.
• DNA-Lipofectamine® 2000 CD complexes can be added directly to cells in culture medium.
• It is not necessary to remove complexes or change/add medium after transfection, but complexes may be removed after 4–6 hours.