Shop All Transfection Reagents

Lipofectamine™ CRISPRMAX™ Cas9 Transfection Reagent (Invitrogen™)

Lipofectamine™ CRISPRMAX™ Cas9 Transfection Reagent is the first optimized lipid nanoparticle transfection reagent for CRISPR-Cas9 protein delivery, providing the cleavage efficiency of electroporation with the simplicity and scalability of a reagent. Lipofectamine CRISPRMAX transfection reagent is an ideal alternative to electroporation as it is gentler on cells and more cost effective overall.

Deliver our superior GeneArt™ Platinum™ Cas9 Nuclease as well as other CRISPR-Cas9 proteins with a reagent that provides:

• Demonstrated cleavage efficiency—tested in over 20 cell types including iPSC, mESC, N2A, CHO, A549, HCT116, HeLa, HEK293, and several others
• Low cell toxicity—less cells needed to initiate your experiment
• Cost savings—whether cost-per-reaction or initial investment
• Easily scalability—an ideal delivery solution for high throughput experiments

Achieve high transfection efficiency in novel genome editing applications
Lipofectamine CRISPRMAX transfection reagent increases the likelihood of successful cleavage and recombination, especially when combined with our GeneArt Platinum Cas9 Nuclease. This ultimately maximizes the efficiency of performing genetic modifications, simplifying your workflow when compared to electroporation.

Improve experimental outcomes using GeneArt Platinum Cas9 Nuclease
Unlike CRISPR plasmid or Cas9 mRNA, using Cas9 protein provides superior cleavage efficiency in primary cells and stem cells. It eliminates the need for transcription or translation of the payload, removes the risk of genomic integration, and is cell cycle independent.

ExpiFectamine™ CHO Transfection Kit (Gibco™)

The ExpiFectamine™ CHO Transfection Kit is a core component of the ExpiCHO™ Expression System Kit (Cat. No. A29133). It is designed for transient transfection of high-density cultures of Chinese hamster ovary (CHO) cells. The high-efficiency, cationic transfection reagent, coupled with an optimized transfection enhancer and culture feed, will power the highest possible level of protein expression from ExpiCHO-S™ Cells (Cat. No. A29127) cultured in ExpiCHO™ Expression Medium (Cat. No. A2910001).

The ExpiFectamine CHO Transfection Kit:
• Achieves greater than 90% transfection efficiency of high-density suspension CHO cell culture, with matching transfection enhancer and culture feed to boost transfection performance and protein expression
• Requires one-third to one-half less plasmid DNA than other transfection reagents while achieving superior expression performance
• Enables options for three transient expression protocols for maximal work flexibility while still fitting into the same overall workflow used by other transient expression systems, such as FreeStyle™ MAX CHO and Expi293™
• Delivers robust and reproducible transfection results, giving you greater confidence in your results
• Demonstrates linear scalability of transfections from culture volumes of <1 mL to >10 L

The ExpiFectamine CHO Transfection Kit is specifically designed to work with ExpiCHO-S Cells in the ExpiCHO™ Expression System Kit. The transfection kit supplies the cationic ExpiFectamine™ CHO Transfection Reagent, ExpiFectamine™ CHO Transfection Enhancer, and ExpiCHO Feed.

Lipofectamine™ 2000 CD Transfection Reagent (Invitrogen™)

Lipofectamine® 2000 CD (Chemically Defined) Transfection Reagent is a proprietary animal origin-free formulation for transfecting nucleic acids into eukaryotic cells. Using Lipofectamine® 2000 CD reagent for transfection provides the following advantages:

• Highest transfection efficiency in many cell types and formats.
• The animal origin-free formulation ensures that mammalian cell culture and bioproduction processes are free of animal-derived materials.
• DNA-Lipofectamine® 2000 CD complexes can be added directly to cells in culture medium.
• It is not necessary to remove complexes or change/add medium after transfection, but complexes may be removed after 4–6 hours.

Lipofectamine™ 3000 Transfection Reagent (Invitrogen™)

Lipofectamine® 3000 Transfection Reagent leverages our most advanced lipid nanoparticle technology to provide superior transfection performance with improved application outcomes and reproducible results. This reagent delivers superior transfection efficiency and improved cell viability for the widest range of hard-to-transfect and common cells (e.g., HEK293, HeLa).

Successfully transfect the widest variety of biologically relevant cell types with a reagent that offers:

• Superior performance—our highest efficiency reagent for difficult-to-transfect cells
• Improved cell viability—gentle on your cells, with low toxicity
• Versatile—one reagent for DNA, RNA, and co-transfection

Efficiently Transfect Difficult-to-Transfect Cells
Lipofectamine® 3000 reagent is designed to efficiently transfect difficult-to-transfect cells, yielding superior transfection performance across the broadest array of cell types. Successfully transfected cell types include:



Fibroblast (3T3, COS-7)Myoblast (C2C12, L6 CRL-1458)
Hepatocyte (HepG2, HuH7)Erythroleukemic cell (K562)
Breast carcinoma (MCF7, Hs578T)Prostate carcinoma (LNCap)
Lung carcinoma (A549, NCI-H460)Osteosarcoma (U-2 OS, Saos-2)
Colon carcinoma (Caco2, SW480)Pancreatic carcinoma (PANC-1)
Skin melanoma (SK-MEL-28) 


View performance data for 60+ cell lines plus comparison to Lipofectamine 2000 >

Transfect Common Cells Efficiently and Cost-Effectively
For common cell types, Lipofectamine® 3000 reagent is more cost-effective than other reagents due to its high concentration and low usage amount. The 1.5 mL size provides up to 1500 transfection reactions (in 24-well plates).

Achieve Superior Transfection Efficiency While Maintaining Low Toxicity
Lipofectamine® 3000 reagent maintains a high transfection efficiency within a robust dynamic range of lipid doses for quick and easy optimization. A low toxicity lipid dose (0.75 µL for 24-well plates) is suggested for applications requiring minimal disruption of the cells.

Enable High Transfection Efficiency in Novel Genome Editing Applications
Lipofectamine® 3000 reagent is suitable for novel genome editing applications. It increases the likelihood of successful cleavage and recombination with TALENs or CRISPRs through highly efficient transfection, ultimately maximizing the efficiency of genetic modifications and simplifying the downstream processes.

Optifect™ Transfection Reagent (Invitrogen™)

Optifect™ Transfection Reagent is a pre-optimized formulation designed to ensure highly efficient gene delivery to a wide variety of cell lines transfected at low confluence or density (10%-70%) (Figure 1). Optifect™ Reagent can also be used with cells that are sensitive to Lipofectamine™ 2000 or other commercially available transfection reagents. Optifect™ Reagent facilitates high levels of protein expression from sparsely plated cells (Table 1). Useful in applications such as studies of cell surface proteins, post-transfection proliferation assays, or other situations where cells must be allowed to grow significantly following transfection. Optifect™ has been shown to be less toxic to cells compared to other commercially available transfection reagents.

Lipofectamine™ Stem Transfection Reagent (Invitrogen™)

Lipofectamine Stem Transfection Reagent is optimized to achieve maximum efficiency with minimal early differentiation in a wide range of stem cells. It can co-deliver DNA, RNA, and Cas9 ribonucleoprotein (RNP) complexes. Lipofectamine Stem reagent is compatible with a variety of media systems, including feeder-free, helping support and simplify your stem cell culture workflow. Researchers can achieve up to 80% or better transfection efficiency in pluripotent stem cells (PSCs) and neural stem cells (NSCs), and up to 45% in mesenchymal stem cells (MSCs).

Transfecting stem cells without inhibiting cell viability and cell growth can be challenging due to the sensitivity of these cells. Lipofectamine Stem is a low toxicity reagent, requiring low amounts of nucleic acid, allowing your stem cells to stay healthy and continue proliferating without inducing differentiation. Lipofectamine Stem reagent is:

Superior in performance—up to 3-fold higher efficiency across iPSCs, hESCs, NSCs, and MSCs compared to other reagents
Versatile—one reagent for DNA, RNA, Cas9 protein, and co-transfection
Gentle on cells—maintains cell proliferation without inducing differentiation
Flexible—transfects cells in adherence or suspension—an alternative to electroporation

Achieve high efficiency gene editing in stem cells
Gene editing applications typically require the ability to co-deliver DNA, RNA, and/or protein into cells. Lipofectamine Stem Transfection Reagent is designed to co-deliver CRISPR/Cas9 complexes into human stem cells for high efficiency gene editing. This one reagent can be used to transfect large DNA plasmids such as all-in-one CRISPR/Cas9 vectors and co-deliver a range of RNAs, such as large Cas9 mRNA with tracr/crRNA complexes or single guide (sg) RNA, and protein such as Cas9 protein combined with tracr/crRNA or sgRNA.

A low-toxicity alternative to electroporation
Transfecting stem cells via electroporation methods can be challenging and cumbersome. Electroporation protocols recommend single cell suspension culture to ensure maximum efficiency and minimal toxicity. Using the reverse transfection protocol, the Lipofectamine Stem Transfection Reagent proves to be an effective and less toxic alternative to electroporation for stem cells. It requires low amounts of nucleic acid to achieve maximum transfection efficiency, on par with electroporation, while supporting continued proliferation without inducing differentiation.

Lipofectamine™ RNAiMAX Transfection Reagent (Invitrogen™)

Lipofectamine® RNAiMAX Transfection Reagent provides the highest transfection efficiencies on the widest variety of cell types for siRNA-mediated gene knockdown experiments. Lipofectamine® RNAiMAX is a proprietary RNAi-specific cationic lipid formulation designed specifically for the delivery of siRNA and miRNA into all cell types.

With Lipofectamine® RNAiMAX Transfection Reagent you will get:

• Superior transfection efficiency requiring lower RNAi concentrations, leading to more effective gene knockdown with minimal nonspecific effects
• Easy optimization due to minimal cytotoxicity across a 10-fold concentration range of transfection reagent
• Superior transfection efficiencies for miRNA antagonists and mimics
• Compatibility with a broad range of cell types, providing the most versatile approach to all of your gene silencing experiments
• A simple and rapid protocol for consistent and reproducible results

High knockdown in a wide range of cells
Lipofectamine® RNAiMAX Transfection Reagent transfects a wide range of cell types (see figure). For gene silencing, Lipofectamine® RNAiMAX Transfection Reagent's high-efficiency transfections lead to the high levels of gene knockdown needed to achieve convincing results.

Simple, high-throughput–ready transfections
Simply mix Lipofectamine® RNAiMAX Transfection Reagent with siRNA, add to your cells, incubate, and measure gene knockdown. The simplicity and speed combined with high transfection efficiency make Lipofectamine® RNAiMAX Transfection Reagent ideal for high-throughput siRNA transfections. Transfection conditions can be easily established for automated or robotic systems used in such applications.

Find an optimized Lipofectamine® RNAiMAX transfection protocol for your cell line.

siPORT™ Amine Transfection Agent (Invitrogen™)

A polyamine-based Ambion® siRNA transfection agent that can be used to transfect cells during subculturing-—saving a full day of valuable time—without increasing toxicity. It is supplied in one tube containing 0.4 mL. A successful siRNA experiment requires the appropriate transfection agent for your cells, and one that is optimized for siRNA delivery. The efficiency with which different transfection reagents deliver nucleic acids can vary dramatically with the type of nucleic acid being transfected (RNA vs. DNA, single-stranded vs. double-stranded) and the cells being used.

Accessory Products:
siPORT™ NeoFX™ Transfection Agent (SKU#s AM4511 and AM4510), a cationic and neutral lipid mixture, is also available. For testing which transfection agent is best with your system, the Silencer® siRNA Transfection II Kit (SKU# AM1631) is recommended. It contains the two different transfection agents along with controls for transfection optimization.

Lipofectamine™ MessengerMAX™ Transfection Reagent (Invitrogen™)

Lipofectamine® MessengerMAX mRNA Transfection Reagent delivers amazing transfection efficiency in neurons and a broad-spectrum of primary cells, enabling improved application outcomes and more biologically relevant research. That's because our novel lipid nanoparticle technology is optimized to deliver the highest amount of mRNA possible without the nuclear entry step that is required with DNA.

Successfully transfect more predictive cell models with a reagent that provides:
• Amazing transfection efficiency in neurons and primary cell types
• Faster protein expression with no risk of genomic integration
• Up to 10x higher cleavage using mRNA CRISPRs

Enable high transfection efficiency in novel genome editing applications
Lipofectamine® MessengerMAX™ reagent helps increase the likelihood of successful cleavage and recombination with GeneArt® CRISPR Nuclease mRNA through highly efficient transfection, ultimately maximizing the efficiency of genetic modifications and simplifying the downstream processes.

Efficiently transfect difficult-to-transfect cells
Lipofectamine® MessengerMAX™ reagent is designed to transfect a higher amount of mRNA into neurons and a broad spectrum of difficult-to-transfect primary cells. This eliminates the need for electroporation or viruses, and provides a > 2-fold improvement in transfection efficiency compared to other lipid based reagents.

Leverage advantages of mRNA transfection
Transfection of mRNA does not require nuclear uptake, which results in faster protein expression than DNA transfection since translation of mRNA occurs in the cytoplasm. Also, mRNA has no risk of genomic integration and transfection efficiency is cell cycle independent.

Oligofectamine™ Transfection Reagent (Invitrogen™)

Oligofectamine™ Transfection Reagent is a proprietary formulation for transfecting oligonucleotides and short interfering RNA (siRNA) into eukaryotic cells. Oligofectamine™ Transfection Reagent forms stable complexes with oligos, permitting efficient transfection into eukaryotic cells in a highly specific, yet nontoxic fashion. Oligofectamine™ Reagent is suitable for nuclear and cytoplasmic targets and transfects a wide variety of cell lines including CHO, HEK-293, NIH 3T3, and HeLa.

Using Oligofectamine™ Transfection Reagent
Oligofectamine™ Reagent is easy to use because it provides a simple and fast protocol. Just dilute Oligofectamine™ Reagent, mix with oligonucleotide, and add to your cells. Oligofectamine™ Reagent requires nanomolar quantities of antisense oligonucleotide, reducing the amount of valuable oligonucleotide needed by up to a thousand-fold. This makes it ideal for high-throughput applications. In addition, Oligofectamine™ Reagent has also been shown to work for siRNA transfections. We recommend this reagent when performing RNAi knockdown experiments in HeLa cells. Please visit RNAi Central for more information.

TurboFect Transfection Reagent (Thermo Scientific™)

Thermo Scientific TurboFect Transfection Reagent is recommended for efficient transfection of plasmid DNA. Distinct from lipid-based formulas, TurboFect is a sterile solution of a proprietary cationic polymer in water. The polymer forms positively-charged complexes with DNA that are both compact and stable. These complexes protect DNA from degradation and facilitate efficient plasmid delivery into eukaryotic cells.

Highlights

• Excellent transfection efficiency in the presence or absence of serum
• Ready-to-use reagent – no need to reconstitute, dilute, or manipulate
• Simple protocol

High transfection efficiency of plasmid DNA into a wide variety of cell types
• Adherent and suspension cells
• Differentiated and undifferentiated cells

Review our experimental considerations to improve your plasmid transfection results.

ExpiFectamine™ 293 Transfection Kit (Gibco™)

The ExpiFectamine™ 293 Transfection Kit is a core component of the Expi293™ Expression System. It is designed for transient transfection of high density cultures of HEK (human embryonic kidney) 293 cells. The high-efficiency, cationic, lipid-based transfection reagent and transfection enhancers are designed to power the highest possible level of protein expression from Expi293F™ cells cultured in Expi293™ Expression Medium

Designed specifically for transfection of high density suspension cell culture, with matching transfection enhancers that boost transfection performance and protein expression
Achieves protein yields 2- to 10-fold higher than other transfection reagents used on high density 293 cell cultures
Employs the same transient expression protocols typically used in current low-density 293 suspension culture systems to easily switch from low density systems to the high yield, high density Expi293™ Expression System
Provides robust and reproducible transfection results, giving users greater confidence in their results
Scale transfections for culture volumes of less than 1 mL to greater than 10 liters, while maintaining equivalent volumetric protein yields

The ExpiFectamine™ 293 Transfection Kit is specifically designed to work with Expi293F™ cells in the Expi293™ Expression System. The transfection kit consists of the cationic lipid-based ExpiFectamine™ 293 Reagent and ExpiFectamine™ 293 Transfection Enhancers 1 and 2. The enhancers are added 16 to 18 hours post-transfection to boost transfection, cell viability, and protein expression. The protocol for use of ExpiFectamine™ 293 Transfection components is very similar to transfection protocols for other transient expression reagents, such as 293fectin and FreeStyle™ MAX.

DMRIE-C Transfection Reagent (Invitrogen™)

DMRIE-C Transfection Reagent is suitable for transfecting DNA and RNA into eukaryotic cells, and is particularly effective for transfecting suspension cells (e.g., Jurkat) and other lymphoid-derived cell lines. Refer to the Cell Lines database at www.invitrogen.com for a list of cell types successfully transfected. DMRIE-C can also be used for in vivo delivery of DNA. DMRIE-C is a 1:1 (M/M) liposome formulation of the cationic lipid DMRIE (1,2-dimyristyloxy-propyl-3-dimethyl-hydroxy ethyl ammonium bromide) and cholesterol in membrane-filtered water.

Lipofectin™ Transfection Reagent (Invitrogen™)

Lipofectin® Transfection Reagent is the reagent of choice for transfection of endothelial cells. Lipofectin® Transfection Reagent is also suitable for transfecting DNA, RNA, and oligonucleotides into mammalian cells, and DNA into plant protoplasts. Lipofectin® reagent has also been shown to work well, in combination with PLUS® Reagent, for the transfection of HeLa cells. Lipofectin® Transfection Reagent is a 1:1 (w/w) liposome formulation of the cationic lipid N-[1-(2,3-dioleyloxy)propyl]-n,n,n-trimethylammonium chloride (DOTMA) and dioleoyl phophotidylethanolamine (DOPE) in membrane-filtered water.

siPORT™ NeoFX™ Transfection Agent (Invitrogen™)

This Ambion® lipid-based formulation can be used to efficiently transfect adherent cells with siRNA as they are subcultured—without increased cytotoxicity. It is provided in one tube containing 0.4 mL.

• Versatile—works with a broad range of cell lines
• Reproducible—produces consistent results, lot-to-lot, plate-to-plate, and well-to-well
• Efficient—performs with high siRNA transfection efficiency, allowing use of low siRNA concentrations to minimize nonspecific effects
• Fast—reverse-transfects cells as they are plated, saving a day

Gene silencing experiments often call for critical, but time-consuming optimization experiments. siPORT™ NeoFX™ Transfection Agent refines siRNA transfection protocols resulting in less optimization. From start to finish, successful gene silencing experiments can be completed in as early as 24 hours. This streamlined protocol can be adapted to a wide range of cells and experimental designs, including high-throughput applications.

Reverse-Transfect Cells as They are Plated, Saving a Day
Just add siPORT™ NeoFX™ to your diluted siRNA, incubate to form transfection complexes, add the complexes to the culture wells, and overlay with cells. This new streamlined protocol, named "neofection", will save researchers a full day of valuable time when compared to traditional plated transfection procedures. The transfection complexes are active and stable even in the presence of serum, so there is no need to remove or replace media following transfection.

Optimized for Lower siRNA Concentrations and High Reproducibility
Recent reports indicate that siRNA concentrations of 100 nM or higher can lead to nonspecific changes in gene expression (off-target effects) in mammalian cultured cells. Reducing the amount of siRNA used for transfections to 1–20 nM minimizes these nonspecific effects, while still providing extensive silencing of the target gene. siPORT™ NeoFX™ Transfection Agent efficiently transfects low concentrations of siRNA. Furthermore, this agent delivers high performance among multiple wells for replicate samples and between different lots of the transfection agent.

Compatible with a Broad Range of Cell Lines
The agent has been used successfully to deliver siRNA into many cell types, including MCF-7 (human breast adenocarcinoma), HT-29 (human colorectal adenocarcinoma), HeLa-S3 (human cervix adenocarcinoma), HeLa (human cervix adenocarcinoma), HepG2 (human hepatocellular carcinoma), BJ (human foreskin fibroblast), A549 (human lung carcinoma), UMR106 (rat osteosarcoma), SKOv3 (human ovarian carcinoma), and SKNAS (human bone marrow neuroblastoma).

Accessory Products:
siPORT™ Amine Transfection Agent (SKU#s AM4502 and AM4503), a polyamine agent, is also available. For testing which transfection agent is best with your system, the Silencer® siRNA Transfection II Kit (SKU# AM1631) is recommended. It contains the two different transfection agents along with controls for transfection optimization.