August 2019 New Products

Ion AmpliSeq™ Mouse BCR IGH SR Assay, DNA Ion Torrent™

The Ion AmpliSeq Mouse BCR IGH SR Assay, DNA, is a robust, targeted next-generation sequencing (NGS) assay for use in basic and translational immunology, immuno-oncology, hemato-oncology, and vaccine research. It is designed to accurately identify and measure the clonal expansion of B lymphocytes in blood, peripheral blood leukocytes (PBLs), peripheral blood mononuclear cells (PBMCs), and fresh-frozen (FF) and formalin-fixed paraffin-embedded (FFPE) samples. The assay identifies unique B-cell clones through targeting of the highly diverse complementarity-determining region 3 (CDR3) of the B-cell receptor (BCR) immunoglobulin heavy (IGH) chain from genomic DNA template. The nucleotide sequence of the IGH CDR3 region serves as a natural barcode to enable clone tracking and measurements of B-cell clonal expansion and diversity. Analysis of IGH CDR3-region amino acid motifs may reveal signatures of B-cell responses to defined antigens. For RNA samples, please see Cat. No. A45487.

Benefits of the Ion AmpliSeq Mouse BCR IGH SR Assay, DNA, include:
Compatibility with a vast array of research sample types, including FFPE tissue, fresh-frozen (FF) tissue, whole blood, PBLs, and PBMCs
High sensitivity and low limit of detection (LoD) for rare clone identification through dual-barcode indexing
Efficient workflow with 48 hour sample-to-results time
Flexible input requirements ranging from 100 ng to 2 µg
Streamlined and user-friendly informatics solution with automated clonotyping, clonal lineage analysis, reporting of key repertoire features, and multi-sample analysis capability to enable tracking of B-cell clones across mouse research samples

The Ion AmpliSeq Mouse BCR IGH SR Assay with DNA input uses multiplex PCR primers to generate 90-bp amplicons that can be sequenced on all chip types supported by the Ion GeneStudio S5 sequencing systems, allowing you to pick the best multiplexing configuration for your unique sample batching needs and throughput requirements. The entire workflow, from library preparation to analysis of samples, can be accomplished in two days using the Ion Chef templating system and Ion GeneStudio S5 system. The assay kit provides a single pool of multiplex PCR primers, dNTPs, and library reagents.

The Ion AmpliSeq Mouse BCR IGH SR Assay, DNA, supports key applications in immunology, immuno-oncology, hemato-oncology, and vaccine research. The high-sensitivity dual-barcode indexing, flexible input requirements (100 ng–2 µg), compatibility with degraded materials, and high-throughput capability make this assay ideal for a range of research applications, including investigations into the role of B cells in anti-cancer immune responses to immunotherapies utilizing checkpoint blockade inhibitors, cancer vaccines, and chimeric antigen receptor (CAR) T cells, as well as infectious and autoimmune disease basic research.

Note: Information about data analysis using Ion Reporter Software v5.12 can be found in the User Guide below.

Pierce™ Dye and Biotin Removal Spin Columns, 0.5 mL Thermo Scientific™

The Thermo Scientific Pierce Dye and Biotin Removal columns enable fast and efficient removal of non-reacted fluorescent dyes, biotin, reducing agents, and crosslinkers from protein samples. Removing free-dye after a labeling reaction is often difficult and time-consuming but is essential for accurate determination of dye-to-protein ratios. The Pierce Dye and Biotin Removal resin is highly specialized to produce exceptional protein recoveries while effectively removing non-conjugated fluorescent dyes, biotinylation reagents, reducing agents, and crosslinkers. Using the appropriate amount of sample and buffer conditions, almost any fluorescent dye can be removed with these columns. Even dilute protein samples (25 µg/mL) can be successfully processed to remove dyes, biotin, reducing agents, and crosslinkers and to efficiently recover proteins (>7 kDa). A single 0.5-mL Dye and Biotin Removal Spin Column can process a 50 to 120 µL of sample. This product is not recommended for desalting or buffer exchange.

Features of Pierce Dye and Biotin Removal columns:
Versatile—removes unreacted fluorescent dyes, biotinylation reagents, crosslinkers, and reducing agents from protein solutions
High recovery—low-binding resin maximizes protein recovery
Easy-to-use—no cumbersome column preparation or equilibration
Fast—small-molecule removal and protein recovery in less than 15 mins
Flexible—available in spin columns and filter spin plates for a range of needs

Available formats:
• Spin columns: 0.5, 2, 5, and 10 mL
• 96-well filter plates: pre-dispensed; compatible with centrifugation for manual or automated purification; enable fast, consistent well-to-well and plate-to-plate reproducibility for small-scale, high-throughput clean-ups

Applications:
• Ideal for post-reaction clean-up of samples
• To effectively remove non-conjugated dye, biotin, reducing agents, and crosslinkers with exceptional protein recovery

Ion AmpliSeq™ Mouse BCR IGH SR Assay, RNA Ion Torrent™

The Ion AmpliSeq Mouse BCR IGH SR Assay, RNA, is a robust, targeted next-generation sequencing (NGS) assay for use in basic and translational immunology, immuno-oncology, hemato–oncology, and vaccine research. It is designed to accurately identify and measure the clonal expansion of B lymphocytes in blood, peripheral blood leukocytes (PBLs), peripheral blood mononuclear cells (PBMCs), and fresh-frozen (FF) and formalin-fixed paraffin-embedded (FFPE) samples. The assay identifies unique B-cell clones through targeting of the highly diverse complementarity-determining region 3 (CDR3) of the B-cell receptor (BCR) immunoglobulin heavy (IGH) chain from genomic DNA template . The nucleotide sequence of the IGH CDR3 region serves as a natural barcode to enable clone tracking and measurements of B-cell clonal expansion and diversity. Analysis of IGH CDR3-region amino acid motifs may reveal signatures of B-cell responses to defined antigens.

Benefits of the Ion AmpliSeq Mouse BCR IGH SR Assay, RNA, include:
Compatibility with a vast array of research sample types, including FFPE tissue, fresh-frozen (FF) tissue, whole blood, PBLs, and PBMCs
High sensitivity and low limit of detection (LoD) for rare clone identification through dual-barcode indexing
Efficient workflow with 48 hour sample-to-results time
Flexible input requirements ranging from 100 ng to 1 µg
Streamlined and user-friendly informatics solution with with multi-sample analysis functionality

The Ion AmpliSeq Mouse BCR IGH SR Assay, RNA, provides a single pool of multiplex PCR primers and library reagents to generate 90-bp amplicons that can be sequenced on all chip types supported by the Ion GeneStudio S5 sequencing systems, allowing you to pick the best multiplexing configuration for your unique sample batching needs and throughput requirements. The entire workflow, (from library preparation to analysis of samples, can be accomplished in two days using the Ion Chef templating system and Ion GeneStudio S5 system..

The Ion AmpliSeq Mouse BCR IGH SR Assay, RNA, with its high sensitivity and specificity, supports key applications in immunology, immuno-oncology, hemato-oncology, and vaccine research. The high-sensitivity dual-barcode indexing, flexible input requirements (100 ng–1 µg), high-depth sequencing, and high-throughput capability make this assay ideal for testing variety of hypothesis for basic or translational biomarker research. A small fragment-size requirement for the input materials for library creation and high multiplexing on the Ion 530 Chip of the Ion GeneStudio S5 sequencer enables researchers to focus on testing a variety of use cases in mouse models, including the role of T cells in generating immune response to immunotherapies such as checkpoint blockade inhibitors, cancer vaccines, or chimeric antigen receptor (CAR) T-cell therapies.

Note: Information about data analysis using Ion Reporter Software v5.12 can be found in the User Guide below.

Ion AmpliSeq™ Mouse TCR Beta SR Assay, DNA Ion Torrent™

The Ion AmpliSeq Mouse TCR Beta SR Assay is a robust, targeted next-generation sequencing (NGS) assay designed to accurately identify and measure the clonal expansion of T lymphocytes by targeting the complementarity-determining region 3 (CDR3) of the T-cell receptor (TCR) gene locus from gDNA input. The assay can be used for basic and translational research to identify T-cell clones since the nucleotide sequence of the CDR3 region is unique to each T cell and codes for the part of the TCR beta chain that is involved in antigen recognition. For RNA samples, please see Cat. No. A45489.

The high sensitivity and specificity of the assay enables key applications in immunology, immuno-oncology, and vaccine research. The high-sensitivity dual-barcode indexing, flexible input requirements (100 ng–1 µg), high-depth sequencing, and high-throughput capability make this assay ideal for testing a variety of use cases in mouse models, including the role of T cells in generating immune response to immunotherapies such as checkpoint blockade inhibitors, cancer vaccines, or chimeric antigen receptor (CAR) T-cell therapies.

Benefits of the Ion AmpliSeq Mouse TCR Beta SR Assay, DNA, include:
Compatibility with a vast array of research sample types, including fresh-frozen and FFPE tissue, whole blood, peripheral blood leukocytes, and peripheral blood mononuclear cells
High sensitivity and low limit of detection for rare clone identification through dual-barcode indexing
Efficient workflow with 48-hour sample-to-results time
Flexible input requirements ranging from 100 ng to 1 µg
Streamlined and user-friendly informatics solution with multi-sample analysis functionality

The Ion AmpliSeq TCR Beta-SR Mouse Kit, DNA, provides a single pool of multiplex PCR primers and library reagents to generate 90-bp amplicons that can be sequenced on all chip types supported by the Ion GeneStudio S5 sequencing systems, allowing you to pick the best multiplexing configuration for your unique sample batching needs and throughput requirements. The entire workflow from library preparation to analysis of samples can be accomplished in two days using the Ion Chef templating system and the Ion GeneStudio S5 sequencing system.

Learn more about the assay ›

Note: Information about data analysis using Ion Reporter Software v5.12 can be found in the User Guide below.

Pierce™ Dye and Biotin Removal Filter Plates, 96 well Thermo Scientific™

The Thermo Scientific Pierce Dye and Biotin Removal columns enable fast and efficient removal of non-reacted fluorescent dyes, biotin, reducing agents, and crosslinkers from protein samples. Removing free-dye after a labeling reaction is often difficult and time-consuming but is essential for accurate determination of dye-to-protein ratios. The Pierce Dye and Biotin Removal resin is highly specialized to produce exceptional protein recoveries while effectively removing non-conjugated fluorescent dyes, biotinylation reagents, reducing agents, and crosslinkers. Using the appropriate amount of sample and buffer conditions, almost any fluorescent dye can be removed with these columns. Even dilute protein samples (25 µg/mL) can be successfully processed to remove dyes, biotin, reducing agents, and crosslinkers and to efficiently recover proteins (>7 kDa). Each well of the Pierce Dye and Biotin Removal 96-well filter plate can process a 40 to 100 µL of sample. This product is not recommended for desalting or buffer exchange.

Features of Pierce Dye and Biotin Removal columns:
Versatile—removes unreacted fluorescent dyes, biotinylation reagents, crosslinkers, and reducing agents from protein solutions
High recovery—low-binding resin maximizes protein recovery
Easy-to-use—no cumbersome column preparation or equilibration
Fast—small-molecule removal and protein recovery in less than 15 mins
Flexible—available in spin columns and filter spin plates for a range of needs

Available formats:
• Spin columns: 0.5, 2, 5, and 10 mL
• 96-well filter plates: pre-dispensed; compatible with centrifugation for manual or automated purification; enable fast, consistent well-to-well and plate-to-plate reproducibility for small-scale, high-throughput clean-ups

Applications:
• Ideal for post-reaction clean-up of samples
• To effectively remove non-conjugated dye, biotin, reducing agents, and crosslinkers with exceptional protein recovery

Pierce™ Dye and Biotin Removal Spin Columns, 2 mL Thermo Scientific™

The Thermo Scientific Pierce Dye and Biotin Removal columns enable fast and efficient removal of non-reacted fluorescent dyes, biotin, reducing agents, and crosslinkers from protein samples. Removing free-dye after a labeling reaction is often difficult and time-consuming but is essential for accurate determination of dye-to-protein ratios. The Pierce Dye and Biotin Removal resin is highly specialized to produce exceptional protein recoveries while effectively removing non-conjugated fluorescent dyes, biotinylation reagents, reducing agents, and crosslinkers. Using the appropriate amount of sample and buffer conditions, almost any fluorescent dye can be removed with these columns. Even dilute protein samples (25 µg/mL) can be successfully processed to remove dyes, biotin, reducing agents, and crosslinkers and to efficiently recover proteins (>7 kDa). A single 2-mL Dye and Biotin Removal Spin Column can process a 400 to 700 µL of sample. This product is not recommended for desalting or for buffer exchange

Features of Pierce Dye and Biotin Removal columns:
Versatile—removes unreacted fluorescent dyes, biotinylation reagents, crosslinkers, and reducing agents from protein solutions
High recovery—low-binding resin maximizes protein recovery
Easy-to-use—no cumbersome column preparation or equilibration
Fast—small-molecule removal and protein recovery in less than 15 mins
Flexible—available in spin columns and filter spin plates for a range of needs

Available formats:
• Spin columns: 0.5, 2, 5, and 10 mL
• 96-well filter plates: pre-dispensed; compatible with centrifugation for manual or automated purification; enable fast, consistent well-to-well and plate-to-plate reproducibility for small-scale, high-throughput clean-ups

Applications:
• Ideal for post-reaction clean-up of samples
• To effectively remove non-conjugated dye, biotin, reducing agents, and crosslinkers with exceptional protein recovery

Pierce™ Dye and Biotin Removal Spin Columns, 5 mL Thermo Scientific™

The Thermo Scientific Pierce Dye and Biotin Removal columns enable fast and efficient removal of non-reacted fluorescent dyes, biotin, reducing agents, and crosslinkers from protein samples. Removing free-dye after a labeling reaction is often difficult and time-consuming but is essential for accurate determination of dye-to-protein ratios. The Pierce Dye and Biotin Removal resin is highly specialized to produce exceptional protein recoveries while effectively removing non-conjugated fluorescent dyes, biotinylation reagents, reducing agents, and crosslinkers. Using the appropriate amount of sample and buffer conditions, almost any fluorescent dye can be removed with these columns. Even dilute protein samples (25 µg/mL) can be successfully processed to remove dyes, biotin, reducing agents, and crosslinkers and to efficiently recover proteins (>7 kDa). A single 5-mL Dye and Biotin Removal Spin Column can process a 1 to 2 mL of sample. This product is not recommended for desalting or buffer exchange.

Features of Pierce Dye and Biotin Removal columns:
Versatile—removes unreacted fluorescent dyes, biotinylation reagents, crosslinkers, and reducing agents from protein solutions
High recovery—low-binding resin maximizes protein recovery
Easy-to-use—no cumbersome column preparation or equilibration
Fast—small-molecule removal and protein recovery in less than 15 mins
Flexible—available in spin columns and filter spin plates for a range of needs

Available formats:
• Spin columns: 0.5, 2, 5, and 10 mL
• 96-well filter plates: pre-dispensed; compatible with centrifugation for manual or automated purification; enable fast, consistent well-to-well and plate-to-plate reproducibility for small-scale, high-throughput clean-ups

Applications:
• Ideal for post-reaction clean-up of samples
• To effectively remove non-conjugated dye, biotin, reducing agents, and crosslinkers with exceptional protein recovery

Pierce™ Dye and Biotin Removal Spin Columns, 10 mL Thermo Scientific™

The Thermo Scientific Pierce Dye and Biotin Removal columns enable fast and efficient removal of non-reacted fluorescent dyes, biotin, reducing agents, and crosslinkers from protein samples. Removing free-dye after a labeling reaction is often difficult and time-consuming but is essential for accurate determination of dye-to-protein ratios. The Pierce Dye and Biotin Removal resin is highly specialized to produce exceptional protein recoveries while effectively removing non-conjugated fluorescent dyes, biotinylation reagents, reducing agents, and crosslinkers. Using the appropriate amount of sample and buffer conditions, almost any fluorescent dye can be removed with these columns. Even dilute protein samples (25 µg/mL) can be successfully processed to remove dyes, biotin, reducing agents, and crosslinkers and to efficiently recover proteins (>7 kDa). A single 10-mL Dye and Biotin Removal Spin Column can process a 2 to 4 mL of sample. This product is not recommended for desalting or buffer exchange.

Features of Pierce Dye and Biotin Removal columns:
Versatile—removes unreacted fluorescent dyes, biotinylation reagents, crosslinkers, and reducing agents from protein solutions
High recovery—low-binding resin maximizes protein recovery
Easy-to-use—no cumbersome column preparation or equilibration
Fast—small-molecule removal and protein recovery in less than 15 mins
Flexible—available in spin columns and filter spin plates for a range of needs

Available formats:
• Spin columns: 0.5, 2, 5, and 10 mL
• 96-well filter plates: pre-dispensed; compatible with centrifugation for manual or automated purification; enable fast, consistent well-to-well and plate-to-plate reproducibility for small-scale, high-throughput clean-ups

Applications:
• Ideal for post-reaction clean-up of samples
• To effectively remove non-conjugated dye, biotin, reducing agents, and crosslinkers with exceptional protein recovery

Ion AmpliSeq™ Mouse TCR Beta SR Assay, RNA Ion Torrent™

The Ion AmpliSeq Mouse TCR Beta SR Assay is a robust, targeted next-generation sequencing (NGS) assay designed to accurately identify and measure the clonal expansion of T lymphocytes by targeting the complementarity-determining region 3 (CDR3) of the T-cell receptor (TCR) gene locus from total RNA input. The assay can be used for basic and translational research to identify T-cell clones since the nucleotide sequence of the CDR3 region is unique to each T cell and codes for the part of the TCR beta chain that is involved in antigen recognition. For DNA samples please see Cat. No. A45488.

Benefits of the Ion AmpliSeq Mouse TCR Beta SR Assay, RNA, include:
Compatibility with a vast array of research sample types, including FFPE tissue, fresh-frozen (FF) tissue, whole blood, PBLs, and PBMCs
High sensitivity and low limit of detection (LoD) for rare clone identification through dual-barcode indexing
Efficient workflow with 48 hour sample-to-results time
Flexible input requirements ranging from 25 ng to 1 µg
Streamlined and user-friendly informatics solution with with multi-sample analysis functionality

The Ion AmpliSeq Mouse TCR Beta SR Kit, RNA, provides a single pool of multiplex PCR primers and library reagents to generate 90-bp amplicons that can be sequenced on all chip types supported by the Ion GeneStudio S5 sequencing systems, allowing you to pick the best multiplexing configuration for your unique sample batching needs and throughput requirements. The entire workflow from library preparation to analysis of samples can be accomplished in two days using the Ion Chef templating system and Ion GeneStudio S5 system.

The Ion AmpliSeq Mouse TCR Beta SR RNA Assay, with its high sensitivity and specificity, supports key applications in the field of immunology, immuno-oncology, hemato-oncology, and vaccine research. The high-sensitivity dual-barcode indexing, flexible input requirements (100 ng–1 µg), high-depth sequencing, and high-throughput capability make this assay ideal for testing a variety of hypotheses for basic or translational biomarker research. A small fragment size requirement from the input materials for library creation and high multiplexing on the Ion 530 Chip of the Ion GeneStudio S5 sequencers enables researchers to focus on testing a variety of use cases in mouse models including the role of T cells in generating immune response to immunotherapies such as checkpoint blockade inhibitors, cancer vaccines, or chimeric antigen receptor (CAR) T-cell therapies.

Learn more about the assay ›

Note: Information about data analysis using Ion Reporter Software v5.12 can be found in the User Guide below.

Oncomine™ BCR IGH LR Assay, RNA Ion Torrent™

The Ion Torrent Oncomine BCR IGH LR Assay is a robust, targeted next-generation sequencing (NGS) assay designed for use in hematology-oncology, immuno-oncology, infectious disease, and basic immunology research. Unlike other technologies, the Oncomine BCR IGH-LR Assay offers more than 400 base amplicons of library sequencing through long-read sequencing chemistry, enabling comprehensive coverage of the B-cell receptor (BCR) immunoglobin heavy (IGH) chain. The assay kit includes a single pool of multiplex PCR primers, cDNA synthesis kit, and Ion Ampliseq library reagents.

The Oncomine BCR IGH LR Assay is designed to accurately measure clonal expansion, quantify somatic hypermutation, and isotype B lymphocytes using total RNA extracted from bone marrow, whole blood, peripheral blood leukocytes (PBLs), peripheral blood mononuclear cells (PBMCs), or sorted cells in fresh-frozen research specimens. The assay utilizes Ion AmpliSeq multiplex PCR technology. It amplifies framework 1 (FR1) and the constant gene region of the BCR IGH gene to interrogate highly diverse complementarity-determining regions CDR1, CDR2, and CDR3, as well as the CH1 domain of the constant gene to enable distinction of all nine isotypes. IGH CDR-region amino acid motifs may reveal signatures of B-cell responses to defined antigens for use as future markers of autoimmune or infectious disease. Automated clonal lineage analysis and multi-sample analysis capability facilitates tracking of B-cell clonal evolution arising from somatic hypermutation and class switch recombination in research samples.

Oncomine BCR IGH LR Assay benefits include:
• Extended amplicon allows for quantification of variable gene somatic hypermutation and isotype identification to enable hemato-oncology, immuno-oncology, and infectious disease translational and clinical research
• Assay primers are designed to amplify all variable and joining gene alleles in the gold-standard IMGT database
• RNA input improves detection of changes in plasmablast and plasma cell populations in research samples following immune challenge or administration of immunomodulatory agents
• Superior multiplex PCR design through Ion AmpliSeq technology assures high accuracy and high sensitivity
• Ultra-low substitution error rate minimizes artifacts arising from sequencing errors to enable highly accurate assessment of somatic hypermutation and clonal heterogeneity in malignant B-cell research samples of interest
• The entire workflow from isolation of RNA to analysis of samples can be accomplished within two days using the Ion Chef templating system and Ion Genestudio S5 sequencing system
• Flexible input requirements ensure successful library construction with as low as 25 ng and up to 2 µg total RNA input
• Streamlined and user-friendly informatics solution offers automated clonotyping, somatic hypermutation quantification, clonal lineage analysis, reporting of key repertoire features, and multi-sample analysis capability to track B-cell clones across research samples

Learn more about the assay ›

Note: Information about data analysis using Ion Reporter Software v5.12 can be found in the User Guide below.

CTS™ StemPro™ HSC Expansion Medium Gibco™

CTS StemPro HSC Expansion Medium was designed to help meet regulatory compliance requirements for ancillary materials and provides more functional cells for cell therapy applications. This xeno-free, serum-free medium is manufactured in accordance with cGMP for medical devices (ISO13485 guidelines). The phenotypes of the hematopoietic stem cells (HSCs) targeted for expansion are the total CD34+ cell population with an emphasis on enrichment of the CD34+CD90+CD45RA- cell subset. CTS StemPro HSC Expansion Medium can be used to study hematopoietic cells from human mobilized peripheral blood, bone marrow, or cord blood.

CTS StemPro HSC Expansion Medium consists of a liquid basal medium and a frozen supplement that is added to the basal medium at the time of use. Growth factors or cytokines are not included.

Features of CTS StemPro Expansion Medium include:
• Manufactured following cGMP for medical devices and designed to help meet regulatory requirements for ancillary materials used in cell and gene therapy manufacturing
• Superior expansion of total CD34+ cell population and enrichment of CD34+CD90+CD45RA- subset
• Maintenance of in vivo functionality as demonstrated in animal engraftment models

Designed to help meet regulatory requirements
CTS StemPro HSC Expansion Medium is manufactured in accordance with cGMP for medical devices and supported by a drug master file (DMF) and regulatory support file (RSF) to assist in global regulatory filings and approvals. The formulation is serum-free and xeno-free, and USP/EP grade components are preferentially chosen where possible. In addition, each lot undergoes quality control testing to ensure safety and performance for cell and gene therapy applications.

Superior expansion
CD34+ cells and the CD34+CD90+CD45RA- subset are found in low proportions in mobilized peripheral blood, bone marrow, and cord blood. They are important for proper hematopoietic and immune function and are studied in a variety of research areas, including transplantation biology, stem cell biology, and hematopoietic development. CTS StemPro HSC Expansion Medium enables superior expansion of not only the total CD34+ compartment, but also enriches for CD34+CD90+CD45RA- stem cells, providing more cells to work with (see figures below).

Maintenance of functionality
Functionality of expanded HSCs is critical to success in cell and gene therapy applications. The most widely accepted method for functional testing of HSCs is engraftment in mouse models. The theory is that if human cells persist after transplantation into immunodeficient mice, the cells have demonstrated long term repopulation and differentiation capacity, which are both defining factors of stem cells. See the figure below for representative mouse model engraftment data of CD34+ cells expanded in CTS StemPro HSC Expansion Medium.

dNTP Mix (10 mM each) Thermo Scientific™

Thermo Scientific dNTP Mix contains premixed aqueous solutions of dATP, dCTP, dGTP and dTTP, each at a final concentration of 10 mM. The nucleotides have greater than 99% purity, are free of nuclease activities, human and E. coli DNA. Mixes offer the possibility to reduce the number of pipetting steps and the risk of reaction set up errors. They are designed for many different molecular biology applications.

Highlights

• Greater than 99% purity confirmed by HPLC
• Free of human and E. coli DNA
• Highly stable
• Stable for years at -20°C
• Stable after multiple freeze-thaw cycles
• Up to 95% of dNTPs remain in triphosphate form even after 7 weeks at room temperature
• Up to 90% of dNTPs remain in triphosphate form after 30 cycles of PCR (1 minute at 94°C; 3 minutes at 72°C)

Application tested in:
• Long range PCR (40 kb)
• cDNA synthesis and RT-PCR
• Real-time PCR
• Standard PCR
• High fidelity PCR

Applications
• For use in all molecular biology applications, including PCR, real-time PCR, high fidelity and long PCR, LAMP-PCR, cDNA synthesis, RT-PCR, RDA, MDA, DNA labeling, and DNA sequencing.

dNTP Mix (10 mM ea) Invitrogen™

Invitrogen 10 mM dNTP Mix is a mixture of four nucleotides (dATP, dCTP, dGTP, dTTP) in 0.6 mM Tris-HCl. Each nucleotide is at a concentration of 10 mM. 10 mM dNTP mix is suitable for use in polymerase chain reaction (PCR), sequencing, fill-in reactions, nick translation, cDNA synthesis, and TdT-tailing reactions.

Invitrogen dNTP manufacturing meets the highest industry standards:
• Manufactured under ISO 9001
• Manufactured in Class D clean rooms

Features include:
• Chemically synthesized
• pH 7.5
• >99% purity confirmed by HPLC
• Stable for 2 years at –20°C
• Free from qPCR, PCR, reverse transcription inhibitors
• Free of DNases and RNases
• Free of human and E. coli DNA

Applications:
• qPCR, RT-qPCR
• PCR, RT-PCR, cDNA synthesis
• High-fidelity and long-range PCR
• Isothermal amplification
• DNA labeling
• Cloning
• Sanger and next-generation sequencing (NGS) sequencing

dNTPs in a different concentration or format ›

Oncomine™ BCR IGH SR Assay, RNA Ion Torrent™

The Oncomine BCR IGH-SR Assay, RNA, is a robust, targeted next-generation sequencing (NGS) assay designed for hematology-oncology and minimal residual disease (MRD) monitoring research, pharmacodynamics and biomarker analysis of immunotherapy, as well as infectious and autoimmune disease research. The assay kit includes a single pool of multiplex PCR primers, total RNA-to-cDNA synthesis kit and Ion AmpliSeq library reagents.

The assay accurately identifies and measures clonal expansion of B lymphocytes using total RNA from bone marrow, whole blood, peripheral blood leukocytes (PBLs), peripheral blood mononuclear cells (PBMCs), and fresh-frozen and formalin-fixed paraffin-embedded (FFPE) research samples. The assay identifies unique B-cell clones through targeting of the highly diverse complementarity-determining region 3 (CDR3) of the B-cell receptor IGH chain from mRNA template via AmpliSeq multiplex framework 3 and joining gene primers. The nucleotide sequence of the IGH CDR3 region serves as a natural barcode to enable clone tracking for MRD clinical research and measurements of B-cell clonal expansion and diversity for pharmacodynamics and vaccine biomarker research. IGH CDR3 region amino acid motifs may reveal signatures of B-cell responses to defined antigens for use as future markers of autoimmune or infectious disease. RNA input improves detection of changes in plasmablast and plasma cell populations following immune challenge or administration of immunomodulatory agents. For DNA samples, please see Cat. No. A45483.

Benefits of the Oncomine BCR IGH SR Assay, RNA, include:
• High sensitivity and exceptionally low limit of detection for rare clone detection and tracking in research samples via dual-barcode indexing and high template capture efficiency of AmpliSeq multiplex PCR
• Compatibility with an array of research sample types, including fresh-frozen and FFPE tissue, bone marrow, whole blood, PBLs, and PBMCs
• Assay primers design to amplify all variable and joining gene alleles in the gold-standard IMGT database
• Ability to generate large libraries with minimal input requirement, facilitating detection of rare clones at a frequency of 10-6 from a single library preparation
• Efficient workflow using the Ion Chef templating system and Ion GeneStudio S5 sequencing system, enabling library-to-results time within 48 hours
• Flexible input requirements ranging from 25 ng to 2 µg
• Streamlined and user-friendly informatics solution with automated clonotyping, clonal lineage analysis, reporting of key repertoire features, and multi-sample analysis capability to enable longitudinal tracking in research samples
• Ultra-low substitution error rate minimizing artifacts arising from sequencing errors, enabling highly accurate assessment of clonal heterogeneity in malignant B-cell research samples of interest

The Oncomine BCR IGH-SR Assay, RNA, uses multiplex PCR primers to generate 110-bp amplicons that can be sequenced on all chip types supported by the Ion GeneStudio S5 sequencing systems, allowing you to pick the best multiplexing configuration for your unique sample batching needs and throughput requirements.

Learn more about the assay ›

Note: Information about data analysis using Ion Reporter Software v5.12 can be found in the User Guide below.

Click-iT™ EdU Proliferation Assay for Microplates Invitrogen™

The Click-iT EdU Proliferation Assay for Microplates provides a more simplified and robust method for the measurement of mammalian cell proliferation compared to traditional BrdU-based methods. After incorporation of EdU (a modified thymidine analogue) into newly synthesize DNA, horse radish peroxidase (HRP) is covalently attached via the highly specific click reaction. Amplex UltraRed Reagent, an HRP substrate, is then added and the resulting fluorescence signal detected. Because of the highly specific attachment of HRP to EdU, this assay results in highly sensitive and reliable measurement of mammalian cell proliferation in a microplate format.

Click-iT EdU Proliferation Assay for Microplates features include:
Improved performance—direct conjugation of HRP to incorporated EdU improves assay sensitivity and accuracy
Increased reliability—assay format results in low well-to-well variability
Simplified protocol—results in reduced time to data; easier to follow protocol
Multiplex enabled—assay can be easily multiplexed with other cell health reagents resulting in more data per sample

Measuring cell proliferation is a fundamental method of assessing cell health, determining genotoxicity, and evaluating anti-cancer drugs. The most accurate method of measuring proliferation is by direct detection of DNA synthesis. Originally this was accomplished through incorporation of a radioactive nucleoside (e.g., 3H-thymidine). This method was replaced by antibody-based detection of the nucleoside analog bromo-deoxyuridine (BrdU). After incorporation of BrdU into the newly synthesized DNA, denaturation is required to expose the BrdU molecules to the anti-BrdU antibody. The denaturation involves harsh methods (HCl, heat, or enzymes) and is time consuming and difficult to perform consistently.

The Click-iT EdU Proliferation Assay is an alternative to the BrdU assay. The nucleoside analog EdU (5-ethynyl-2´-deoxyuridine) is added to live cells and incorporated into DNA during active DNA synthesis. The incorporated EdU contains an alkyne group which is covalently joined to the azide group present in HRP using a highly specific copper-catalyzed covalent reaction ('click reaction'). Amplex UltraRed Reagent is then added and its conversion by HRP into a highly fluorescent product is recorded using a fluorescence microplate reader (excitation: 568, emission: 585 nm). With a high extinction coefficient, good quantum efficiency, and resistance to auto-oxidation, Amplex UltraRed Reagent delivers higher sensitivity and a broader assay range than other fluorogenic or colorimetric HRP substrates. It also offers versatility through detection by either fluorescence or absorbance measurement.

Compared to anti-BrdU-based microplate proliferation assays, the Click-iT EdU Proliferation Assay uses small reaction moieties and does not require antibodies or DNA denaturation, both of which can reduce assay performance. Additionally, streptavidin-biotin binding is not required, eliminating the necessary biotin blocking steps required by cells possessing high levels of endogenous biotin.

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