Biochemical Toxicology Products

FluoReporter™ Blue Fluorometric dsDNA Quantitation Kit, 200-2,000 assays Invitrogen™

The FluoReporter® Blue Fluorometric dsDNA Quantitation Kit provides the protocols and reagents for analyzing cellular NA with the blue-fluorescent Hoechst 33258 nucleic acid stain. With this kit, quantitation of cellular DNA is rapid, and all manipulations can be carried out in microplate wells. The cells are lysed by freezing them in distilled water, the diluted dye solution is then added to the lysed cells, and fluorescence is measured.

DiFMUP (6,8-Difluoro-4-Methylumbelliferyl Phosphate) Invitrogen™

DiFMUP is a fluorinated 4-methylumbelliferyl phosphate (MUP) derivative that has a lower pKa than that of MUP, making DiFMUP an excellent substrate for continuously assaying acid phosphatases at low pH. The reaction product of DiFMUP has excitation/emission maxima of ~358/450 nm. DiFMUP is also available packaged in ten vials of 10 mg each (D-22065).

Thiol and Sulfide Quantitation Kit Invitrogen™

The Thiol and Sulfide Quantitation Kit provides an ultrasensitive colorimetric assay for quantitating both protein and nonprotein thiols (also called sulfhydryls or mercaptans). In this assay, thiols or inorganic sulfides reduce a disulfide-inhibited derivative of papain, stoichiometrically releasing the active enzyme. The activity of the enzyme is then measured using the chromogenic papain substrate N-benzoyl-L-argining, p-nitroanilide (L-BAPNA). The enzymatic amplification step in this kit enables researchers to detect as little as 0.2 µM thiol - a sensitivity that is about 100-fold greater than that achieved using Ellman's reagent.

MUP (4-Methylumbelliferyl Phosphate, Free Acid) Invitrogen™

MUP is a fluorescent substrate for alkaline phosphatases. Alkaline phosphatase mediated hydrolysis of its phosphate substituent yields the blue-fluorescent 4-methylumbelliferyl (excitation/emission ~386/448 nm).

ATP Determination Kit, 200-1,000 assays Invitrogen™

Molecular Probes® ATP Determination Kit offers a convenient bioluminescence assay for quantitative determination of ATP with recombinant firefly luciferase and its substrate D-luciferin. The assay is based on luciferase’s absolute requirement for ATP in producing light (emission maximum ~560 nm at pH 7.8). This assay is extremely sensitive; most luminometers can detect as little as 0.1 picomole of pre-existing ATP, or ATP as it is being formed in kinetic systems. This sensitivity has led to numerous applications for detecting ATP production in various enzymatic reactions, including ATPase and NADPH oxidase, as well as for detecting low-level bacterial contamination in samples such as blood, milk, urine, soil and sludge. The luciferin–luciferase bioluminescence assay has also been used successfully to study the effects of antibiotics on bacterial populations, to determine cell proliferation and cytotoxicity in both bacterial and mammalian cells, and to distinguish cytostatic versus cytocidal potential of anticancer drugs on malignant cell growth.

ATP Determination Kit Specifications:
• Luminescence is monitored at ~560 nm using a luminometer
• Can detect as little as 0.1 picomole of pre-existing ATP, or ATP as it is being formed in kinetic systems
• Luciferase and luciferin are packaged separately, which enables researchers to optimize conditions for their samples and instruments
• Includes sufficient reagents to perform 200 ATP assays using 500 µL sample volumes or 1,000 ATP assays using 100 µL sample volumes

Find More Viability and Cytotoxicity Assays and Probes for Detecting Enzymes That Metabolize Phosphates
Review Viability and Cytotoxicity Assay Kits for Diverse Cell Types—Section 15.3 and Detecting Enzymes That Metabolize Phosphates and Polyphosphates—Section 10.3 in the Molecular Probes® Handbook for more information on these products.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

Measure-IT™ Thiol Assay Kit Invitrogen™

The Measure-iT™ Thiol Assay Kit proves easy and accurate quantitation of thiol. The assay has a linear range of 0.05-5 uM thiol, making it up to 400 times more sensitive than colorimetric methods based on Ellman's reagent. The assay is performed at room temperature; maximum fluorescence signal is attained in 5 minutes and is stable for at least 1 hour.

PiPer™ Pyrophosphate Assay Kit Invitrogen™

The PiPer™ Pyrophosphate Assay Kit provides a sensitive fluorogenic or chromogenic method for detecting as little as 0.4 µM (fluorescence) or 2 µM (absorbance) inorganic pyrophosphate in a 100 µL assay volume.

PiPer™ Phosphate Assay Kit Invitrogen™

The PiPer Phosphate Assay Kit provides an ultrasensitive fluorometric method for detecting as little as 0.2 µM Pi in purified enzyme systems with a 100 µL assay volume.