Cell-Based Gpcr Reporter Assays - Kits

GeneBLAzer™ D2-Gqo5-NFAT-bla CHO-K1 Cells Thermo Scientific™

For Academic pricing please login or contact us at discoverysciences@invitrogen.com.

GeneBLAzer® D2-Gqo5-NFAT-bla CHO-K1 cells contain the human Dopamine Receptor 2 (D2) stably integrated into the GeneBLAzer® Gqo5-NFAT-bla CHO-K1 cell line. GeneBLAzer® Gqo5-NFAT-bla CHO-K1 cells (K1536) contain a beta-lactamase reporter gene under control of the Nuclear Factor of Activated T-cells (NFAT) response element and the Chimeric G protein, Gqo5.

The GeneBLAzer® D2-Gqo5-NFAT-bla CHO-K1 cells are functionally validated for Z' and EC50 concentrations of apomorphine. In addition, GeneBLAzer® D2-Gqo5-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.

The D2 receptor is found primarily in the brain. There are two forms of D2 which are created by alternate splicing: a short form and a long form. This assay uses the long form, although little difference in ligand binding or signaling has been seen between the two forms.

Tango™ EDG3-bla U2OS Cells Thermo Scientific™

Tango™ EDG3-bla U2OS cells contain the human Endothelial Differentiation Gene 3 (EDG3) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin⁄TEV protease fusion protein and the beta-lactamase reporter gene under the control of a UAS response element. The Tango™ EDG3-bla U2OS cells have been functionally validated for Z’ factor and EC50 concentrations of S1P. In addition, Tango™ EDG3-bla U2OS cells have been tested for assay performance under variable conditions.

Tango™ CXCR3-bla U2OS Cells Thermo Scientific™

The Tango™ CXCR3-bla U2OS cells contain the human chemokine (C-X-C motif) receptor 3 (CXCR3) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin⁄TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.

The Tango™ CXCR3-bla U2OS cells are functionally validated for Z' and EC50 concentrations of I-TAC (CXCL11). In addition, Tango™ CXCR3-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.

For Academic pricing please login or contact us at discoverysciences@invitrogen.com.

GeneBLAzer™ D1-CRE-bla CHO-K1 Cells Thermo Scientific™

GeneBLAzer® D1-CRE-bla CHO-K1 cells contain the human Dopamine Receptor 1 (D1) stably integrated into the CellSensor® CRE-bla CHO-K1 cell line. CellSensor® CRE-bla CHO-K1 cells (Cat. no. K1535) contain a beta-lactamase reporter gene under control of the Cyclic AMP Response Element (CRE) response element.

The GeneBLAzer® D1-CRE-bla CHO-K1cells are functionally validated for Z' and EC50 concentrations of dihydrexidine. In addition, GeneBLAzer® D1-CRE-bla CHO-K1 DA cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.

Agonists and antagonists of dopamine D1 receptors are in various stages of clinical trials for the treatment of neurological disorders like Parkinson's disease, Alzheimer's disease, schizophrenia, and psychosis.

For Academic pricing please login or contact us at discoverysciences@invitrogen.com.

Tango™ EDG7-bla U2OS Cells Thermo Scientific™

Tango™ EDG7-bla U2OS cells contain the human Endothelial Differentiation Gene 7 (EDG7) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin⁄TEV protease fusion protein and the beta-lactamase reporter gene under the control of a UAS response element. The Tango™ EDG7-bla U2OS cells have been functionally validated for Z’ factor and EC50 concentrations of LPA. In addition, Tango™ EDG7-bla U2OS cells have been tested for assay performance under variable conditions.

Tango™ F2RL1-bla U2OS Cells Thermo Scientific™

The Tango™ F2RL1-bla U2OS cells contain the human Coagulation factor II (thrombin) receptor-like 1 (F2RL1) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin⁄TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.

The Tango™ F2RL1-bla U2OS cells are functionally validated for Z' and EC50 concentrations of SLIGRL. In addition, Tango™ F2RL1-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.

For Academic pricing please login or contact us at discoverysciences@invitrogen.com.

Tango™ HTR1A-bla U2OS Cells Thermo Scientific™

Tango™ HTR1A-bla U2OS cells contain the human Serotonin Type 1a receptor (HTR1A) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin⁄TEV protease fusion protein and the beta-lactamase reporter gene under the control of a UAS response element. Tango™ HTR1A-bla U2OS DA cells have been functionally validated for Z’ factor and EC50 concentrations of 5-CT (Figure 1). In addition, Tango™ HTR1A-bla U2OS cells have been tested for assay performance under variable conditions.

GeneBLAzer™ HCRTR1-NFAT-bla CHO-K1 Cells Thermo Scientific™

The GeneBLAzer® HCRTR1-NFAT-bla CHO-K1 cells contain the human Hypocretin (Orexin) Receptor 1 (HCRTR1), (Accession # NM_001525) stably integrated into the CellSensor® NFAT-bla CHO-K1 cell line. CellSensor® NFAT-bla CHO-K1 cells (Cat # K1078) contain a beta-lactamase (bla) reporter gene under control of the NFAT Response Element.

The HCRTR1-NFAT-bla CHO-K1 cells are functionally validated for Z'-factor and EC50 concentrations of Orexin A. In addition, HCRTR1-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section.

GeneBLAzer™ M1-NFAT-bla Jurkat Cells Thermo Scientific™

GeneBLAzer® M1-NFAT-bla Jurkat cells contain the human Acetylcholine (muscarinic) subtype 1 receptor (M1) stably integrated into the CellSensor® NFAT-bla Jurkat cell line. CellSensor® NFAT-bla Jurkat cells (Cat. no. K1077) contain a beta-lactamase (bla) reporter gene under control of the Nuclear Factor of Activated T-cells (NFAT) response element.

M1-NFAT-bla Jurkat cells are functionally validated for Z'-factor and EC50 concentrations of carbachol. In addition, M1-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.

In the brain, M1 activation mediates 'slow' neuronal excitability. Cortical and hippocampal muscarinic receptors are thought to be important in the attentional aspects of cognition. The predominant receptor subtypes in these brain areas are M1, M3, and M4. Therefore, M1 is a potential target for cognition, Alzheimer's, dementia, and schizophrenia. Studies on knock-out mouse models of M1 are also beginning to reveal potential functions of the receptor. Additional information on the muscarinic receptors can be found in reviews.

For Academic pricing please login or contact us at discoverysciences@invitrogen.com.

GeneBLAzer™ T-Rex™ GPR119-CRE-bla CHO-K1 Cells Thermo Scientific™

The GeneBLAzer® T-Rex™ GPR119-CRE-bla CHO-K1 cells contain the human G-Protein Receptor 119 (GPR119), (Accession # NP_848566) stably integrated into the CellSensor® CRE-bla CHO-K1 cell line. CellSensor® CRE-bla CHO-K1 cells (Cat # K1129) contain a beta-lactamase (bla) reporter gene under control of the cAMP response element.

The T-Rex™ GPR119-CRE-bla CHO-K1 cells are functionally validated for Z'-factor and EC50 concentrations of AR231453(1). In addition, T-Rex™ GPR119-CRE-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section.

GeneBLAzer™ FPRL-1-Gα15-NFAT-bla CHO-K1 Cells Thermo Scientific™

GeneBLAzer® FPRL-1-Gα15-NFAT-bla CHO-K1 cells contain the human Formyl Peptide Like Receptor 1 (FPRL-1) stably integrated into the GeneBLAzer® Gα15-NFAT-bla CHO-K1 cell line. GeneBLAzer® Gα15-NFAT-bla CHO-K1 cells (Cat. no. K1537) contain a beta-lactamase reporter gene under control of the Nuclear Factor of Activated T-cells (NFAT) response element and the Promiscuous G protein, Gα15.

The GeneBLAzer® FPRL-1-Gα15-NFAT-bla CHO-K1 cells are functionally validated for Z' and EC50 concentrations of W Peptide. In addition, GeneBLAzer® FPRL-1-Gα15-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.

The FPRL-1 receptor has been implicated in a variety of inflammation and neurological disorders, including Alzheimer's disease.

For Academic pricing please login or contact us at discoverysciences@invitrogen.com.

GeneBLAzer™ PTGIR-CRE-bla CHO-K1 Cells Thermo Scientific™

GeneBLAzer® PTGIR-CRE-bla CHO-K1 cells contain the human Prostaglandin I2 receptor (IP) (PTGIR), (Accession # NM_000960.3) stably integrated into the CellSensor® CRE-bla CHO-K1 cell line. CellSensor® CRE-bla CHO-K1 cells (Cat. no. K1535) contain a beta-lactamase reporter gene under control of the CRE Response Element. The GeneBLAzer® PTGIR-CRE-bla CHO-K1 cells are functionally validated for Z’-factor and EC50 concentrations of Iloprost (Figure 1). In addition, GeneBLAzer® PTGIR-CRE-bla CHO-K1 cells have been tested for assay performance under variable conditions.

GeneBLAzer™ ADORA2A CHO-K1 DA Assay Kit

This cell line is available as dividing cells by requesting a quote for K1245 from dividingcells@invitrogen.com

GeneBLAzer® ADORA2A CHO-K1 DA (Division Arrested) cells and ADORA2A-CRE-bla CHO-K1 cells contain the human Adenosine A2a (ADORA2A) receptor stably integrated into the CellSensor® CRE-bla CHO-K1 cell line. CellSensor® CRE-bla CHO-K1 cells (Cat. no.K1129) contain a beta-lactamase reporter gene under control of the Cyclic AMP Response Element (CRE). Division Arrested (DA) cells are available in two configurations: an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates.

DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both ADORA2A CHO-K1 DA cells and ADORA2A-CRE-bla CHO-K1 cells are functionally validated for Z’-factor and EC50 concentrations of 5’-adenosine (NECA); (Figure 1). In addition, ADORA2A-CRE-bla CHO-K1 cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.

The Adenosine A2a Receptor (ADORA2A) has been shown to have many roles throughout the body. ADORA2A is involved in pain and inflammation regulation, inhibition of platelet aggregation, blood pressure regulation and contributes to ischemic brain damage. Selective antagonists of the ADORA2A pathway are being tested as adjuvants to dopaminergic drugs to treat Parkinson’s disease and schizophrenia.


Storage:

GeneBLAzer® ADORA2A CHO-K1 DA Cells and ADORA2A-CRE-bla CHO-K1 Cells are shipped on dry ice. Store in liquid nitrogen immediately upon receipt or thaw for immediate use.


Contents:
GeneBLAzer® ADORA2A CHO-K1 DA Assay Kit
Each system contains sufficient division arrested (DA) cells & substrate to assay 1 x 384-well plate. (Other materials are required separately; please refer to the protocol).
Includes:
•ADORA2A CHO-K1 DA cells
•LiveBLAzer™-FRET B/G Loading Kit, 70µg
•Solution D, 1ml
•2 protocols
•Certificate of Analysis
GeneBLAzer® ADORA2A CHO-K1 DA cells
Each system contains sufficient division arrested (DA) cells to assay 10 x 384-well plates (LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:
•ADORA2A CHO-K1 DA cells
•1 protocol
•Certificate of Analysis
GeneBLAzer® ADORA2A-CRE-bla CHO-K1 cells
(LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:
•ADORA2A-CRE-bla CHO-K1 cells
•1 protocol
•Certificate of Analysis

GeneBLAzer™ HCRTR2-Gα15-NFAT-bla CHO-K1 Cells Thermo Scientific™

GeneBLAzer® HCRTR2-Gα15-NFAT-bla CHO-K1 cells contain the human Orexin Receptor B (HCRTR2) stably integrated into the GeneBLAzer® Gα15-NFAT-bla CHO-K1 cell line. GeneBLAzer® Gα15-NFAT-bla CHO-K1 cells (Cat. no. K1537) contain a beta-lactamase reporter gene under control of the Nuclear Factor of Activated T-cells (NFAT) response element and the Promiscuous G protein, Gα15.

The GeneBLAzer® HCRTR2-Gα15-NFAT-bla CHO-K1 cells are functionally validated for Z' and EC50 concentrations of Orexin B. In addition, GeneBLAzer® HCRTR2-Gα15-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.

The HCRTR2 receptor has been detected in the brain as well as adrenal gland, gastrointestinal tract, and pancreas. The signaling cascades used by orexin receptors appear to be varied and cell type dependant. In CHO cells, calcium release is seen after agonist treatment. In hypothalamic neurons, the frequency of action potentials is increased. Administering either orexin peptide into the bloodstream of rats caused an increase in feeding behavior. The orexin system is also thought to regulate neuroendocrine function and the sleep wake cycle. HCRTR2 has been directly implicated in narcolepsy in animal studies.

For Academic pricing please login or contact us at discoverysciences@invitrogen.com.

T-REx™ Tango™ P2RY14-bla U2OS Cells Thermo Scientific™

T-REx™ Tango™ P2RY14-bla U2OS cells contain the human purinergic receptor P2Y14 (P2RY14) under doxycycline inducible control linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin⁄TEV protease fusion protein and the beta-lactamase reporter gene under the control of a UAS response element. The T-REx™ Tango™ P2RY14-bla U2OS cells have been functionally validated for Z’ factor and EC50 concentrations of UDP-glucose. In addition, T-REx™ Tango™ P2RY14-bla U2OS cells have been tested for assay performance under variable conditions.
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