Cell-Based Gpcr Reporter Assays - Kits

GeneBLAzer™ Gqi5-NFAT-bla FreeStyle™ 293F Cells Thermo Scientific™

GeneBLAzer® Gqi5-NFAT-bla FreeStyle™ 293F cells contain the chimeric G-protein Gqi5 stably integrated into the CellSensor® NFAT-blaFreeStyle™ 293F cell line. CellSensor® NFAT-bla FreeStyle™ 293F cells contain a beta-lactamase reporter gene under control of a stably integrated NFAT response element. Gqo5-NFAT-bla FreeStyle™ 293F is a parental cell line designed to be used for the construction of Gi/o-coupled GPCR beta-lactamase cellular assays. The Gi/o receptor of interest should be stably expressed in the cell line by expression from the selectable vector pcDNA™6.2-hygro-DEST (Cat. no. K1233). Gqi5-NFAT-bla FreeStyle™ 293F cells have been shown to respond to the agonist of a transiently expressed D2 receptor.

Tango™ EDG4-bla U2OS Cells Thermo Scientific™

The Tango™ EDG4-bla U2OS cells contain the human Endothelial Differentiation Gene 4 (EDG4) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.

The Tango™ EDG4-bla U2OS DA cells have been functionally validated for Z' factor and EC50 concentrations of LPA (18:1). In addition, Tango™ EDG4-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section.

GeneBLAzer™ GPR10-NFAT-bla CHO-K1 Cells Thermo Scientific™

GeneBLAzer® GPR10 CRE-bla CHO-K1 cells contain the human Prolactin Releasing Peptide (GPR10) stably integrated into the CellSensor® CRE-bla CHO-K1 cell line. CellSensor® CRE-bla CHO-K1 cells (Cat. no. K1535) contain a beta-lactamase reporter gene under control of the Cyclic AMP Response Element (CRE) response element.

The GeneBLAzer® GPR10 CRE-bla CHO-K1 cells are functionally validated for Z' and EC50 concentrations of PrRP20. In addition, GeneBLAzer® GPR10 CRE-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.

The GPR10 receptor has been implicated in a variety of metabolic disorders, including lactation, the regulation of food intake, and other physiological functions.

For Academic pricing please login or contact us at discoverysciences@invitrogen.com.

GeneBLAzer™ OXTR-Gqo5-NFAT-bla CHO-K1 Cells Thermo Scientific™

GeneBLAzer® OXTR-Gqo5-NFAT-bla CHO-K1 cells contain the human Oxytocin Receptor (OXTR), (Accession # NM_002531) stably integrated into the GeneBLAzer® Gqo5-NFAT-bla CHO-k1 cell line. GeneBLAzer® Gqo5-NFAT-bla CHO-k1 cells (Cat. no.K1536) contain a beta-lactamase reporter gene under control of the NFAT. The GeneBLAzer® OXTR-Gqo5-NFAT-bla CHO-K1 cells are functionally validated for Z’-factor and EC50 concentrations of Oxytocin.

Tango™ GPR109A-bla U2OS Cells Thermo Scientific™

The Tango™ GPR109A-bla U2OS cells contain the human Nicotinic Acid Receptor (GPR109A) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.

The Tango™ GPR109A-bla U2OS cells are functionally validated for Z' and EC50 concentrations of Nicotinic Acid. In addition, Tango™ GPR109A-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.

For Academic pricing please login or contact us at discoverysciences@invitrogen.com.

Tango™ EDG6-bla U2OS Cells Thermo Scientific™

The Tango™ EDG6-bla U2OS cells contain the human Endothelial Differentiation, Lysophosphatidic Acid G-protein-coupled Receptor, 6 (EDG6) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin⁄TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.

The Tango™ EDG6-bla U2OS cells cells have been functionally validated for Z' factor and EC50 concentrations of established ligands. In addition, Tango™ EDG6-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary for each product.

Tango™ AGTR1-bla U2OS Cells Thermo Scientific™

Tango™ AGTR1-bla U2OS cells contain the human Angiotensin Receptor 1 (AGTR1) (AGTR1) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin⁄TEV protease fusion protein and the beta-lactamase reporter gene under the control of a UAS response element. The Tango™ AGTR1-bla U2OS cells have been functionally validated for Z’ factor and EC50 concentrations of Angiotensin II (Figure 1). In addition, Tango™ AGTR1-bla U2OS cells have been tested for assay performance under variable conditions.

GeneBLAzer™ PTGDR-CRE-bla CHO-K1 Cells Thermo Scientific™

GeneBLAzer™ PTGDR-CRE-bla CHO-K1 cells contain the human Prostanoid (PTGDR) Receptor stably integrated into the CellSensor™ CRE-bla CHO-K1 cell line. CellSensor™ CRE-bla CHO-K1 cells (Cat. no. K1535) contain a beta-lactamase reporter gene under control of the Cyclic AMP Response Element (CRE) response element.

The GeneBLAzer™ PTGDR-CRE-bla CHO-K1cells are functionally validated for Z' and EC50 concentrations of Prostaglandin D2. In addition, GeneBLAzer™ PTGDR-CRE-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.

The PTGDR receptor also known as DP, is a Gs-coupled receptor which activates adenylate cyclase. PTGDR receptors are located in vascular smooth muscle cells and blood platelets, and are also believed to be expressed in the brain, where they may be involved in the regulation of sleep. In addition, knockout studies in mice suggest that PTGDR may be involved in allergic and asthmatic responses. Potent agonists and antagonists for PTGDR have been identified.

Tango™ GPR120S-bla U2OS Cells Thermo Scientific™

Tango™ GPR120 (short)-bla U2OS cells contain the human GPR120 (short) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin⁄TEV protease fusion protein and the beta-lactamase reporter gene under the control of a UAS response element. The Tango™ GPR120 (short)-bla U2OS cells have been functionally validated for Z’ factor and EC50 concentrations of GW9508 (Figure 1). In addition, Tango™ GPR120 (short)-bla U2OS cells have been tested for assay performance under variable conditions.

GeneBLAzer™ GnRHR-NFAT-bla CHO-K1 Cells

GeneBLAzer® GnRHR-NFAT-bla CHO-K1 cells contain the human gonadotropin-releasing hormone receptor (GnRHR), (Accession # NM_001012763) stably integrated into the CellSensor® NFAT-bla HEK 293T cell line. CellSensor® NFAT-bla HEK 293T (Cat No. K1538) cells contain a beta-lactamase reporter gene under control of the NFAT Response Element. GeneBLAzer® GnRHR-NFAT-bla CHO-K1 cells are functionally validated for Z’-factor and EC50 concentrations of Luteinizing Hormone-Releasing Hormone (LH-RH). In addition, GeneBLAzer® GnRHR-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions.

GeneBLAzer™ NTSR1 CHO-K1 DA Assay Kit Thermo Scientific™

The GeneBLAzer® NTSR1 CHO-K1 DA cells contain the human Neurotensin Receptor 1 (NTSR1) stably integrated into the GeneBLAzer® Gqo5-NFAT-bla CHO-K1 cell line. CellSensor® NFAT-bla CHO-K1 cells (Cat. no. K1078) contain a beta-lactamase reporter gene under control of the NFAT response element.

The GeneBLAzer® NTSR1 CHO-K1 DA cells have been functionally validated for Z' factor and EC50 concentrations of established ligands. In addition, GeneBLAzer® NTSR1 CHO-K1 DA cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary for each product. Division Arrested cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.

Tango™ GALR1-bla U2OS Cells Thermo Scientific™

The Tango™ GALR1 -bla U2OS cells contain the human Galanin Receptor 1 (GALR1) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin⁄TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.

The Tango™ GALR1-bla U2OS cells have been functionally validated for Z' factor and EC50 concentrations of established ligands. In addition, Tango™ GALR1-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary for each product.

GeneBLAzer™ CRHR2-CRE-bla CHO-K1 Cells Thermo Scientific™

GeneBLAzer® CRHR2-CRE-bla CHO-K1 cells contain the human Corticotropin Releasing Factor Receptor 2 (CRHR2) stably integrated into the CellSensor® CRE-bla CHO-K1 cell line. CellSensor® CRE-bla CHO-K1 cells (Cat. no. K1535) contain a beta-lactamase reporter gene under control of the Cyclic AMP Response Element (CRE) response element.

The GeneBLAzer® CRHR2-CRE-bla CHO-K1 cells are functionally validated for Z' and EC50 concentrations of CRF. In addition, GeneBLAzer® CRHR2-CRE-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.

Corticotropin releasing factor is a 41-amino acid peptide that plays a role in the integration of autonomic, neuroendocrine, and behavioral responses to stress. These effects are mediated through two receptor families, CRHR1 and CRHR2. While CRF was originally isolated from the hypothalamus, where it was shown to be the primary neuroregulator mediating the hypothalamic-pituitary-adrenocortical stress axis, it has since been found to be widely distributed outside the hypothalamus throughout the central nervous system. Presently, there are five distinct targets for CRF with unique pharmacology and localization. These have been placed into three distinct classes, two of which are the G-protein-coupled receptors CRF1 (CRHR1) and CRF2 (CRHR2). Three functional splice variants have been identified for the mammalian CRHR2 receptor, although pharmacological characterization of these splice variants revealed no major differences between them.

For Academic pricing please login or contact us at discoverysciences@invitrogen.com.

Tango™ MCHR2-bla U2OS Cells Thermo Scientific™

The Tango™ MCHR2-bla U2OS cells contain the human Melanin-concentrating hormone receptor 2 (MCHR2) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin⁄TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.

The Tango™ MCHR2-bla U2OS cells are functionally validated for Z' and EC50 concentrations of I-TAC (CXCL11). In addition, Tango™ MCHR2-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.

For Academic pricing please login or contact us at discoverysciences@invitrogen.com.

GeneBLAzer™ AVPR2 CHO-K1 DA Assay Kit Thermo Scientific™

GeneBLAzer™ AVPR2 CHO-K1 DA (Division Arrested) cells and AVPR2-CRE-bla CHO-K1 cells contain the human Arginine Vasopressin 2 (AVPR2) receptor stably integrated into the CellSensor™ CRE-bla CHO-K1 cell line. CellSensor™ CRE-bla CHO-K1 cells (Cat. no.K1129) contain a beta-lactamase reporter gene under control of the Cyclic AMP Response Element (CRE). Division Arrested (DA) cells are available in two configurations: an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate) or a tube of cells sufficient to analyze 10 x 384-well plates.

DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both AVPR2 CHO-K1 DA cells and AVPR2-CRE-bla CHO-K1 cells are functionally validated for Z’-factor and EC50 concentrations of dDAVP (Figure 1). In addition, AVPR2-CRE-bla CHO-K1 cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.

Agonists and antagonists of the AVPR2 receptors are in various stages of clinical trials for the treatment of hypertension and as diuretics.


Storage:

GeneBLAzer™ AVPR2 CHO-K1 DA Cells and AVPR2-CRE-bla CHO-K1 Cells are shipped on dry ice. Store in liquid nitrogen immediately upon receipt or thaw for immediate use.


Contents:
GeneBLAzer™ AVPR2 CHO-K1 DA Assay Kit
Each system contains sufficient division arrested (DA) cells & substrate to assay 1 x 384-well plate. (Other materials are required separately; please refer to the protocol).
Includes:
•AVPR2 CHO-K1 DA cells
•LiveBLAzer™-FRET B/G Loading Kit, 70µg
•Solution D, 1ml
•2 protocols
•Certificate of Analysis
GeneBLAzer™ AVPR2 CHO-K1 DA cells
Each system contains sufficient division arrested (DA) cells to assay 10 x 384-well plates (LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:
•AVPR2 CHO-K1 DA cells
•1 protocol
•Certificate of Analysis
GeneBLAzer™ AVPR2-CRE-bla CHO-K1 cells
(LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:
•AVPR2-CRE-bla CHO-K1 cells
•1 protocol
•Certificate of Analysis

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