February 2020 New Products

GeneArt™ Gibson Assembly HiFi Master Mix Invitrogen™

GeneArt Gibson Assembly HiFi Master Mix enables DNA assembly and cloning via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a one-step, 15–60 minute isothermal reaction. DNA fragments of different lengths are uniformly assembled using complementary overlaps between fragments. The inherent flexibility of this approach is suitable for small and large DNA constructs and includes both single and multiple inserts. The resulting products can be used for a variety of downstream applications including transformation, PCR and rolling circle amplification (RCA). The GeneArt Gibson Assembly HiFi Master Mix kit includes master mix, positive control, and water and accommodates the use of your own competent cells.

Features of the GeneArt Gibson Assembly HiFi Master Mix include:
Simple—seamlessly assemble and clone up to six DNA fragments in a single reaction
Efficient—high fidelity provides more correct clones than other methods
Flexible—design guidelines allow assembly into any vector of your choice
Convenient—available as master mix kits and cloning kits complete with chemical or electrocompetent cells
Trusted—over 4000 citations in the scientific literature highlighting great success

Seamless assembly of multiple fragments
GeneArt Gibson Assembly HiFi cloning is a simple, one-step process whereby up to six fragments are combined in a proprietary enzymatic mix in order to assemble DNA fragments with shared terminal end homology without leaving any extra sequences or scars behind (seamless). After a 15–60 minute incubation, a portion of the assembly reaction is then transformed into chemically competent or electrocompetent cells, yielding clones that are ready for analysis the next day. The required 20- to 40-base pair end homology is designed into the de novo fragment for synthesis or can be easily engineered by PCR amplification with custom DNA oligos. This special enzyme mix creates a seamless and covalently bound DNA construct providing high efficiency.

Robust method for maximum efficiency
Due to the covalently bound final product, the GeneArt Gibson Assembly HiFi method allows the utilization of chemically competent cells or electrocompetent cells for the highest transformation efficiency, improving the success of finding the right clone, particularly for more challenging constructs. There is no need for restriction enzymes, ligation, or recombination sites, and the method provides a perfectly seamless construct without unwanted extra bases.

Provides great versatility
The GeneArt Gibson Assembly HiFi method provides versatility, can streamline many techniques through the rapid combination, addition, deletion, or exchange of DNA segments, and eliminates the need to subclone, saving time and effort in the cloning workflow.

Convenient formats
GeneArt Gibson Assembly HiFi kits are available in multiple formats, including:
GeneArt Gibson Assembly HiFi Cloning Kit, chemically competent cells (with One Shot TOP10 chemically competent cells)
GeneArt Gibson Assembly HiFi Cloning Kit, electrocompetent cells (with ElectroMAX DH10B electrocompetent cells)
• GeneArt Gibson Assembly HiFi Master Mix kits (cloning kit without the cells) (this page)

GeneArt™ Gibson Assembly EX Cloning Kit, chemically competent cells Invitrogen™

The GeneArt Gibson Assembly EX Cloning Kit enables DNA assembly via a technique that allows up to 15 overlapping DNA fragments to be seamlessly linked in a single-tube, two-step optimized reaction. DNA fragments of different lengths are uniformly assembled using complementary overlaps between fragments. The inherent flexibility and success of this approach is suitable for small and large DNA constructs, includes both single and multiple inserts, and has been used to build entire genomes. The resulting products can be used for a variety of downstream applications, including transformation, PCR, and rolling circle amplification (RCA). The GeneArt Gibson Assembly EX Cloning Kit, chemically competent cells, is a complete kit that includes master mix, positive control, water, and One Shot TOP10 chemically competent E. coli.

Features of the GeneArt Gibson Assembly EX Cloning Kit include:
Simple—seamlessly assemble and clone up to 15 DNA fragments in a single reaction
Efficient—robust efficiency provides successful clones for both simple and challenging constructs
Flexible—design guidelines allow assembly into any vector of your choice
Convenient—available as master mix kits and cloning kits complete with chemical or electrocompetent cells
Trusted—over 4000 citations in the scientific literature highlighting great success

Seamless assembly of up to 15 fragments
GeneArt Gibson Assembly EX cloning is a robust, single-tube, two-step process whereby up to 15 inserts and vector are combined in a proprietary enzymatic mix in order to assemble DNA fragments with shared terminal end homology without leaving any extra sequences or scars behind (seamless). After this dually optimized reaction is complete, a portion of the assembly reaction is then transformed into chemically competent or electrocompetent cells, yielding clones ready for analysis the next day. The required 20- to 40-base pair end homology is designed into the de novo fragment for synthesis or can be easily engineered by PCR amplification with custom DNA oligos. This special enzyme mix creates a seamless and covalently bound DNA construct providing high efficiency.

Robust method for maximum efficiency
Due to the covalently bound final product, the GeneArt Gibson Assembly EX method allows the utilization of chemically competent cells or electrocompetent cells for the highest transformation efficiency, especially important for challenging constructs. There is no need for restriction enzymes, ligation, or recombination sites, and the method provides a perfectly seamless construct without unwanted extra bases.

Provides great versatility
The GeneArt Gibson Assembly EX method provides versatility, can streamline many techniques through the rapid combination, addition, deletion, or exchange of DNA segments, and eliminates the need to subclone, saving time and effort in the cloning workflow.

Convenient formats
GeneArt Gibson Assembly EX kits are available in multiple formats, including:
• GeneArt Gibson Assembly EX Cloning Kit, chemically competent cells (with One Shot TOP10 chemically competent cells) (this page)
GeneArt Gibson Assembly EX Cloning Kit, electrocompetent cells (with ElectroMAX DH10B electrocompetent cells)
GeneArt Gibson Assembly EX Master Mix kits (cloning kit without the cells)

GeneArt™ Gibson Assembly EX Cloning Kit, electrocompetent cells Invitrogen™

The GeneArt Gibson Assembly EX Cloning Kit enables DNA assembly via a technique that allows up to 15 overlapping DNA fragments to be seamlessly linked in a single-tube, two-step optimized reaction. DNA fragments of different lengths are uniformly assembled using complementary overlaps between fragments. The inherent flexibility and success of this approach is suitable for small and large DNA constructs, includes both single and multiple inserts, and has been used to build entire genomes. The resulting products can be used for a variety of downstream applications, including transformation, PCR, and rolling circle amplification (RCA). The GeneArt Gibson Assembly EX Cloning Kit, electrocompetent cells, is a complete kit that includes master mix, positive control, water, and ElectroMAX DH10B electrocompetent E. coli.

Features of the GeneArt Gibson Assembly EX Cloning Kit include:
Simple—seamlessly assemble and clone up to 15 DNA fragments in a single reaction
Efficient—robust efficiency provides successful clones for both simple and challenging constructs
Flexible—design guidelines allow assembly into any vector of your choice
Convenient—available as master mix kits and cloning kits complete with chemical or electrocompetent cells
Trusted—over 4000 citations in the scientific literature highlighting great success

Seamless assembly of up to 15 fragments
GeneArt Gibson Assembly EX cloning is a robust, single-tube, two-step process whereby up to 15 inserts and vector are combined in a proprietary enzymatic mix in order to assemble DNA fragments with shared terminal end homology without leaving any extra sequences or scars behind (seamless). After this dually optimized reaction is complete, a portion of the assembly reaction is then transformed into chemically competent or electrocompetent cells, yielding clones ready for analysis the next day. The required 20- to 40-base pair end homology is designed into the de novo fragment for synthesis or can be easily engineered by PCR amplification with custom DNA oligos. This special enzyme mix creates a seamless and covalently bound DNA construct providing high efficiency.

Robust method for maximum efficiency
Due to the covalently bound final product, the GeneArt Gibson Assembly EX method allows the utilization of chemically competent cells or electrocompetent cells for the highest transformation efficiency, especially important for challenging constructs. There is no need for restriction enzymes, ligation, or recombination sites, and the method provides a perfectly seamless construct without unwanted extra bases.

Provides great versatility
The GeneArt Gibson Assembly EX method provides versatility, can streamline many techniques through the rapid combination, addition, deletion, or exchange of DNA segments, and eliminates the need to subclone, saving time and effort in the cloning workflow.

Convenient formats
GeneArt Gibson Assembly EX kits are available in multiple formats, including:
• GeneArt Gibson Assembly EX Cloning Kit, electrocompetent cells (with ElectroMAX DH10B electrocompetent cells) (this page)
GeneArt Gibson Assembly EX Cloning Kit, chemically competent cells (with One Shot TOP10 chemically competent cells)
GeneArt Gibson Assembly EX Master Mix kits (cloning kit without the cells)

GeneArt™ Gibson Assembly EX Master Mix Invitrogen™

GeneArt Gibson Assembly EX Master Mix enables DNA assembly via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a single-tube, two-step optimized reaction. DNA fragments of different lengths are uniformly assembled using complementary overlaps between fragments. The inherent flexibility and success of this approach is suitable for small and large DNA constructs, includes both single and multiple inserts, and has been used to build entire genomes. The resulting products can be used for a variety of downstream applications, including transformation, PCR, and rolling circle amplification (RCA). The GeneArt Gibson Assembly EX Master Mix kit includes master mix, positive control, and water, and accommodates the use of your own competent cells.

Features of the GeneArt Gibson Assembly EX Master Mix include:
Simple—seamlessly assemble and clone up to 15 DNA fragments in a single reaction
Efficient—robust efficiency provides successful clones for both simple and challenging constructs
Flexible—design guidelines allow assembly into any vector of your choice
Convenient—available as master mix kits and cloning kits complete with chemical or electrocompetent cells
Trusted—over 4000 citations in the scientific literature highlighting great success

Seamless assembly of up to 15 fragments
GeneArt Gibson Assembly EX cloning is a robust, single-tube, two-step process whereby up to 15 inserts and vector are combined in a proprietary enzymatic mix in order to assemble DNA fragments with shared terminal end homology without leaving any extra sequences or scars behind (seamless). After this dually optimized reaction is complete, a portion of the assembly reaction is then transformed into chemically competent or electrocompetent cells, yielding clones ready for analysis the next day. The required 20- to 40-base pair end homology is designed into the de novo fragment for synthesis or can be easily engineered by PCR amplification with custom DNA oligos. This special enzyme mix creates a seamless and covalently bound DNA construct providing high efficiency.

Robust method for maximum efficiency
Due to the covalently bound final product, the GeneArt Gibson Assembly EX method allows the utilization of chemically competent cells or electrocompetent cells for the highest transformation efficiency, especially important for challenging constructs. There is no need for restriction enzymes, ligation, or recombination sites, and the method provides a perfectly seamless construct without unwanted extra bases.

Provides great versatility
The GeneArt Gibson Assembly EX method provides versatility, can streamline many techniques through the rapid combination, addition, deletion, or exchange of DNA segments, and eliminates the need to subclone, saving time and effort in the cloning workflow.

Convenient formats
GeneArt Gibson Assembly EX kits are available in multiple formats, including:
GeneArt Gibson Assembly EX Cloning Kit, electrocompetent cells (with ElectroMAX DH10B electrocompetent cells)
GeneArt Gibson Assembly EX Cloning Kit, chemically competent cells (with One Shot TOP10 chemically competent cells)
• GeneArt Gibson Assembly EX Master Mix kits (cloning kit without the cells) (this page)

CaptureSelect™ FcXP Affinity Matrix Thermo Scientific™

CaptureSelect FcXP Affinity Matrix is the next-generation CaptureSelect FcXL matrix, specifically designed for higher binding capacity. The selectivity remains identical; the ligand recognizes the CH3 domain of all human IgG subclasses (IgG1, 2, 3, and 4), while maintaining the elution characteristics of CaptureSelect FcXL Affinity Matrix, but the binding capacity is increased (>40 g IgG/L).

Features of this affinity matrix include:
• Mild elution, making it suitable for Fc fusion proteins
• Human-specific, no binding to bovine antibodies
• Excellent scalability
• Non-animal derived

CaptureSelect FcXP Affinity Matrix can be used for the purification of IgG and Fc fusion proteins directly from complex source materials and enables a robust and efficient purification process with excellent purity obtained in one step.

View other research scale antibody affinity resins ›

Free of animal components
Our CaptureSelect products are affinity ligands created by a proprietary technology based on camelid-derived single domain antibody fragments. The ligand is a 13-kDa single-domain fragment comprising the three complementarity determining regions (CDRs) that form the antigen-binding domain, efficiently produced in Saccharomyces cerevisiae in a production process free of animal components.

Main characteristics:

Matrix: agarose-based, epoxide activated
Average particle size: 65 ± 10 µm
Ligand: CaptureSelect FcXP affinity ligand
Ligand coupling method: epoxide coupling
Binding capacity: 40–50 g IgG/L resin depending on flow rate, column height, and contact time
Elution conditions: 20 mM citric acid or acetic acid, pH 3–4, or 100 mM Tris, 2.0 M MgCl2, 40% (v/v) propylene glycol, pH 7
Flow characteristics: 150–300 cm/h (up to 2 bar)
Formulation buffer: 20% (v/v) ethanol

GeneArt™ Gibson Assembly HiFi Cloning Kit, electrocompetent cells Invitrogen™

The GeneArt Gibson Assembly HiFi Cloning Kit enables DNA assembly and cloning via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a one-step, 15–60 minute isothermal reaction. DNA fragments of different lengths are uniformly assembled using complementary overlaps between fragments. The inherent flexibility of this approach is suitable for small and large DNA constructs and includes both single and multiple inserts. The resulting products can be used for a variety of downstream applications including transformation, PCR and rolling circle amplification (RCA). The GeneArt Gibson Assembly HiFi Cloning Kit, electrocompetent cells, is a complete kit that includes master mix, positive control, water, and ElectroMAX DH10B electrocompetent E. coli.

Features of the GeneArt Gibson Assembly HiFi Cloning Kit include:
Simple—seamlessly assemble and clone up to six DNA fragments in a single reaction
Efficient—high fidelity provides more correct clones than other methods
Flexible—design guidelines allow assembly into any vector of your choice
Convenient—available as master mix kits and cloning kits complete with chemical or electrocompetent cells
Trusted—over 4000 citations in the scientific literature highlighting great success

Seamless assembly of multiple fragments
GeneArt Gibson Assembly HiFi cloning is a simple, one-step process whereby up to six fragments are combined in a proprietary enzymatic mix in order to assemble DNA fragments with shared terminal end homology without leaving any extra sequences or scars behind (seamless). After a 15–60 minute incubation, a portion of the assembly reaction is then transformed into chemically competent or electrocompetent cells, yielding clones that are ready for analysis the next day. The required 20- to 40-base pair end homology is designed into the de novo fragment for synthesis or can be easily engineered by PCR amplification with custom DNA oligos. This special enzyme mix creates a seamless and covalently bound DNA construct providing high efficiency.

Robust method for maximum efficiency
Due to the covalently bound final product, the GeneArt Gibson Assembly HiFi method allows the utilization of chemically competent cells or electrocompetent cells for the highest transformation efficiency, improving the success of finding the right clone, particularly for more challenging constructs. There is no need for restriction enzymes, ligation, or recombination sites, and the method provides a perfectly seamless construct without unwanted extra bases.

Provides great versatility
The GeneArt Gibson Assembly HiFi method provides versatility, can streamline many techniques through the rapid combination, addition, deletion, or exchange of DNA segments, and eliminates the need to subclone, saving time and effort in the cloning workflow.

Convenient formats
GeneArt Gibson Assembly HiFi kits are available in multiple formats, including:
• GeneArt Gibson Assembly HiFi Cloning Kit, electrocompetent cells (with ElectroMAX DH10B electrocompetent cells) (this page)
GeneArt Gibson Assembly HiFi Cloning Kit, chemically competent cells (with One Shot TOP10 chemically competent cells)
GeneArt Gibson Assembly HiFi Master Mix kits (cloning kit without the cells)

TaqMan™ 2019nCoV Control Kit v1 Thermo Scientific™

The Applied Biosystems TaqMan 2019-nCoV Control Kit v1 is a synthetic positive control that contains target sequences for each of the assays included in the TaqMan 2019-nCoV Assay Kit v1 (Cat. No. A47532). The control includes synthetic DNA target sequences for three SARS-CoV-2 genes (ORF1ab, S protein, and N protein) and the human RNase P RPPH1 gene.

During RT-PCR, use 1 µL of the 2019-nCoV Control v1 as the sample for each positive-control reaction.

The control can be used in SARS-CoV-2 research to verify assay performance and to help with troubleshooting.

4N6FLOQSwabs™, regular tip, 2-mL cuvette, peel-pouch Applied Biosystems™

4N6FLOQSwabs, regular tip, are comprised of a solid, molded plastic applicator shaft with a regular-sized tip. The tip of the applicator is coated with short Nylon fibers that are arranged in a perpendicular fashion. This perpendicular arrangement results from a process called flocking, where the fibers are sprayed onto the tip of the swab while it is held in an electrostatic field. This process creates a highly absorbent, thin layer with an open structure. The swab is supplied in a peelpouch with a 2-mL cuvette and is suitable for reference and evidence collection applications.

Key features:
• Certified free of DNase, RNase, and amplifiable human DNA
• Rapid absorption capability and superior sample release
• Regular-size tip for buccal cell collection
• Individually packaged in a convenient peel-pouch format
• Break point (from tip end): 20 mm

Unlike traditional fiber wound swabs, 4N6FLOQSwabs have no internal absorbent core to disperse and entrap the specimen—the sample stays close to the surface for faster and more efficient elution. The perpendicular Nylon fibers act like a soft brush that facilitates improved collection of cellular material. Capillary action between the fiber strands facilitates strong hydraulic uptake of liquid sample, and the sample stays close to the surface allowing easy elution.

4N6FLOQSwabs are manufactured by Copan, a market leader in the area of sample collection tools, and distributed globally by Thermo Fisher Scientific.

GeneArt™ Gibson Assembly HiFi Cloning Kit, chemically competent cells Invitrogen™

The GeneArt Gibson Assembly HiFi Cloning Kit enables DNA assembly and cloning via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a one-step, 15–60 minute isothermal reaction. DNA fragments of different lengths are uniformly assembled using complementary overlaps between fragments. The inherent flexibility of this approach is suitable for small and large DNA constructs and includes both single and multiple inserts. The resulting products can be used for a variety of downstream applications including transformation, PCR and rolling circle amplification (RCA). The GeneArt Gibson Assembly HiFi Cloning Kit, electrocompetent cells, is a complete kit that includes master mix, positive control, water, and One Shot TOP10 chemically competent E. coli.

Features of the GeneArt Gibson Assembly HiFi Cloning Kit include:
Simple—seamlessly assemble and clone up to six DNA fragments in a single reaction
Efficient—high fidelity provides more correct clones than other methods
Flexible—design guidelines allow assembly into any vector of your choice
Convenient—available as master mix kits and cloning kits complete with chemical or electrocompetent cells
Trusted—over 4000 citations in the scientific literature highlighting great success

Seamless assembly of multiple fragments
GeneArt Gibson Assembly HiFi cloning is a simple, one-step process whereby up to six fragments are combined in a proprietary enzymatic mix in order to assemble DNA fragments with shared terminal end homology without leaving any extra sequences or scars behind (seamless). After a 15–60 minute incubation, a portion of the assembly reaction is then transformed into chemically competent or electrocompetent cells, yielding clones that are ready for analysis the next day. The required 20- to 40-base pair end homology is designed into the de novo fragment for synthesis or can be easily engineered by PCR amplification with custom DNA oligos. This special enzyme mix creates a seamless and covalently bound DNA construct providing high efficiency.

Robust method for maximum efficiency
Due to the covalently bound final product, the GeneArt Gibson Assembly HiFi method allows the utilization of chemically competent cells or electrocompetent cells for the highest transformation efficiency, improving the success of finding the right clone, particularly for more challenging constructs. There is no need for restriction enzymes, ligation, or recombination sites, and the method provides a perfectly seamless construct without unwanted extra bases.

Provides great versatility
The GeneArt Gibson Assembly HiFi method provides versatility, can streamline many techniques through the rapid combination, addition, deletion, or exchange of DNA segments, and eliminates the need to subclone, saving time and effort in the cloning workflow.

Convenient formats
GeneArt Gibson Assembly HiFi kits are available in multiple formats, including:
• GeneArt Gibson Assembly HiFi Cloning Kit, chemically competent cells (with One Shot TOP10 chemically competent cells) (this page)
GeneArt Gibson Assembly HiFi Cloning Kit, electrocompetent cells (with ElectroMAX DH10B electrocompetent cells)
GeneArt Gibson Assembly HiFi Master Mix kits (cloning kit without the cells)

TaqMan™ Fast Advanced Master Mix, no UNG Applied Biosystems™

TaqMan Fast Advanced Master Mix delivers accurate quantification and dependability in a short time. It provides best-in-class performance, including the widest quantifiable range, in both single and duplex reactions, even with challenging targets. TaqMan Fast Advanced Master Mix contains AmpliTaq Fast DNA Polymerase, dNTPs with dUTP, ROX dye (passive reference), and optimized buffer components. It is supplied at a 2X concentration. Note: This TaqMan Fast Advanced Master Mix does not contain uracil-N-glycoslyase (UNG). See a complete listing of all TaqMan Fast Advanced Master Mix products, with and without UNG.

TaqMan Fast Advanced Master Mix features include:
Best-in-class performance—superior sensitivity, accuracy, dynamic range, and specificity compared to standard mixes in standard mode
Engineered for enhanced benchtop stability—stable at room temperature for up to 72 hours in preassembled reactions
Optimized for multiplexing—validated for duplexing with exogenous and endogenous internal positive control assays
Reduced run times (<40 minutes) on fast and standard instrumentation—optimized for fast mode on fast instruments and fast cycling conditions on standard instruments
Seamlessly transitions into your experiments—validated with TaqMan Assays for gene expression and microRNAs, and TaqMan array micro-fluidic cards

Best-in-class performance
TaqMan Fast Advanced Master Mix has been designed to provide performance equal to or better than standard master mixes. It has been benchmarked against the leading suppliers' standard and fast master mixes to help ensure that it succeeds in providing best-in-class sensitivity, accuracy, dynamic range, and specificity. TaqMan Fast Advanced Master Mix’s dynamic range (up to 7 logs) is the widest in the industry. The impressive sensitivity of the mix is showcased in the figure below which compares the Ct values of TaqMan Fast Advanced Master Mix and TaqMan Universal PCR Master Mix across a panel of gene expression assays.

Benchtop stability for high-throughput handling and convenience
TaqMan Fast Advanced Master Mix has been engineered to retain a high level of performance in preassembled reactions for up to 72 hours. The stability of this mix provides users of high-throughput liquid handling systems the assurance that the results on the first plate will mimic those of the last plate. The figure below shows an assay that was run upon assembly (time 0) and after 72 hours of incubation at 30°C, simulating the most extreme room temperature scenario.

For those with less extreme throughput needs, the enhanced stability of this master mix provides an overall added convenience to your workflow, as you are no longer constrained to immediately running plates upon assembly.

Optimized for multiplexing
We realize that confidence is paramount when it comes to your results. TaqMan Fast Advanced Master Mix has been designed to help deliver accurate results for duplex reactions using an internal positive control (IPC). The figure below shows results for the experimental target β-actin (gene name: ACTB), which was serially diluted and amplified in single-target reactions and duplex reactions.

Validated for microRNA assays
TaqMan Fast Advanced Master Mix has been validated for use with multiple real-time PCR applications, including microRNA assays. The formulation provides high specificity and dynamic range, the two most critical performance attributes that define successful results when working with microRNAs. The data in the figure below demonstrate excellent PCR linearity over a 6-log range of input template.

Reduced run times on standard instrumentation
TaqMan Fast Advanced Master Mix has been optimized for use with both fast and standard instrumentation, enabling researchers who currently own standard instruments to also reap the performance benefits and time savings this mix provides. The figure below showcases the impressive results achieved when using TaqMan Fast Advanced Master Mix under fast thermal cycling conditions on the Applied Biosystems 7300 Real-Time PCR System. The mix has been tested with all Applied Biosystems standard real-time PCR instrumentation to enable success independent of whether or not you own a fast-enabled instrument.

SuperScript™ IV CellsDirect™ cDNA Synthesis Kit Invitrogen™

The SuperScript IV CellsDirect cDNA Synthesis Kit is optimized for the synthesis of first-strand cDNA directly from a mammalian cell lysate without first isolating RNA. Cell lysis and reverse transcription are performed in the same tube, and the resulting first-strand cDNA is ready to use in PCR and qPCR.

Features of the SuperScript IV kit include:
• Compatible with a wide range of mammalian cell types grown under different treatment conditions
• Single-tube format minimizes reagent loss, sample loss, and handling time
• Total lysate volume can be used in first-strand cDNA synthesis reaction, providing greater yields with a limited number of cells and allowing for detection of rare transcripts
• High-quality cDNA generated for use in a variety of applications, including PCR and qPCR
• Simple protocol takes around 37 minutes
• Compatible with TaqMan and SYBR Green qPCR master mixes from various manufacturers
• Optimized performance for 1–10,000 cells per sample, results equivalent to those from purified RNA

The SuperScript IV CellsDirect cDNA Synthesis Kit includes SuperScript IV Reverse Transcriptase. Features of this enzyme include:
• Reduced RNase H activity
• Higher thermal stability
• High yields of cDNA in the first-strand synthesis reaction, for greater sensitivity and enhanced detection of rare transcripts
• Superior tolerance to various RT inhibitors

SuperScript IV reverse transcriptase is a top choice for all RT-PCR and qRT-PCR applications. It is a proprietary MMLV mutant with superior robustness and reliability in RT reactions. It is significantly improved over SuperScript III reverse transcriptase in inhibitor resistance, processivity, and reaction speed, while retaining all the benefits of the previous enzyme, including increased thermostability, highly efficient full-length cDNA synthesis, and reduced RNase activity.

How it works
In traditional RT-PCR, RNA is first isolated from cells in a time-consuming procedure that can lead to a loss of material. Using the SuperScript IV CellsDirect cDNA Synthesis Kit, the cells are lysed and the cDNA is generated from the lysate in a single tube with minimal handling and no sample loss. DNase I treatment is an optional step and can be used to eliminate genomic DNA prior to first-strand synthesis. This kit has been optimized for small cell samples, ranging from 10,000 cells down to a single cell.

4N6FLOQSwabs® Buccal Lollipop, single-sided, peelpouch Applied Biosystems™

4N6FLOQSwabs Buccal Lollipops, single-sided, have a solid molded plastic applicator shaft with a round-shaped tip to improve collection of buccal cells from the inside of a subject’s check. One side of the tip of the 4N6FLOQSwabs Buccal Lollipop are coated with short Nylon fibers arranged in a perpendicular fashion to create a highly absorbent thin layer with an open structure. Collection of cellular material on one side of the lollipop allows for increased transfer efficiency of the sample. The perpendicular Nylon fibers act like a soft brush that facilitates improved collection of cellular material. Capillary action between the fiber strands facilitates strong hydraulic uptake of liquid sample, and the sample stays close to the surface for easy elution.

Key features:
• Certified free of DNase, RNase, and amplifiable human DNA
• Rapid absorption capability and superior sample release
• Single sided lollipop for buccal cell collection
• Individually packaged in a convenient peel pouch format

4N6FLOQSwabs Buccal Lollipops are manufactured by Copan, a market leader in the area of sample collection tools, and distributed globally by Thermo Fisher Scientific.

CytKick™ Max Autosampler Invitrogen™

The CytKick Max Autosampler is for use with the Attune NxT Acoustic Focusing Cytometer. It enables rapid processing of multiple samples by automatically analyzing samples from 96- and 384-well plates (standard and deep), as well as some custom plate types and microfuge tube racks. The CytKick Max Autosample features a boost mode that enables faster sample processing, as well as passive cooling for some plates/racks. A CytKick Max Accessory Kit is included that contains four accessory plates: two cooling plates and two tube adaptors, as well as a Max dongle key. A computer is not included with the autosampler since one is provided with the cytometer.

Features of the CytKick Max Autosampler include:
Reliable—intelligent probe design minimizes clogging and carryover (0.5%) and prevents damage to the instrument
Mixing by aspiration—mixing of sample by aspiration instead of shaking ensures homogeneity of the sample and maintains cell viability
Easy transition—no disassembly, no additional QC, no reboot required for conversion between plates and tubes
Boost mode—reduces processing time from 42 min to 22 min for 96-well plate (using one rinse and one mix and full analysis of a 20-µL sample)
Sample cooling—passive cooling available for 96-well U-bottom plates and microfuge tube racks
Maximum compatibility—96- and 384-well plates (standard and deep), 96- and 384-well standard-depth foil-covered plates, some custom plate types, and 1.5-mL and 2-mL microfuge tube racks (up to 24 per rack)
Auto-cleaning—performs automated cleaning when the instrument is shutting down

High-throughput mode
The CytKick Max Autosampler has two options for processing samples in a high-throughput environment: high-throughput mode and boost mode. High-throughput mode enables plate screening by collecting 20 µL of sample at a rate of 1000 µL/min, while Boost mode shortens the time between samples and allows the user to select the sample volume and sample input rate.

Reliable
The CytKick Max Autosampler was designed to minimize system down time. Automated sampling devices are often plagued by clogging or probe crashes. The Intelligent Probe, with a 200-micron diameter that is larger than most probes available in the industry, minimizes instances of clogging.

To prevent probe crashes, which may require expensive probe replacement, the CytKick Max Autosampler automatically performs a probe self-calibration to help ensure consistent positioning of the probe within every well. The self-calibration verifies proper function of the probe, reduces variability in probe positioning over time to prevent failures during plate runs, and helps improve reliability over the life of the system. Self-calibration of the probe also allows the autosampler to be easily moved, so it can be shared between multiple instruments.

Sample cooling
The CytKick Max Autosampler is compatible with a 96-well standard-plate universal cooling block that will keep samples at 2-8°C for ~60 minutes, a 96-well standard-plate extended cooling block that will keep samples at 2-8°C for ~100 minutes, and a microfuge-tube cooling rack that will keep samples at 2-8°C for ~100 minutes. The user will need to chill the cooling block/rack in the refrigerator overnight prior to use.

Maximum compatibility
The CytKick Max Autosampler allows use of many different plate formats, including 96-, 384-, and deep-well plates, without being restricted to a single manufacturer. The CytKick Max Autosampler also enables 1.5- and 2.0-mL microfuge tubes to be run in an automated format. In addition, the autosampler initiates automated cleaning procedures when the instrument is shutting down to help ensure proper maintenance with little user interaction.

Mixing by aspiration
Unlike other plate loaders that mix samples by shaking, which may not ensure homogeneity of the sample, the CytKick Max Autosampler mixes samples by aspiration and stirring, enabling uniform mixing while preserving sample viability and resulting in consistent data with CVs less than 10% across the plate. The CytKick Max Autosampler also has a gentle mix mode for fragile cell lines.

Easy to transition
To maintain system flexibility, the manual sample probe is always accessible even when the loader is present, and switching between tubes and plates is detected via software and does not require additional user intervention.

Simple to operate
The instrument design and software were developed with customer ease-of-use in mind. The CytKick Max Autosampler has fluidic bottles with sensors that inform the user when the fluids need to be replaced. The autosampler’s indicator lights and warning systems have been designed to ensure that a user who has walked away from the system can easily see the system status at all times.

Setting up an experiment with the Attune NxT cytometric software is simple. Using a plate layout view, users can create compensation wells, define instrument settings, and identify samples. The intuitive software allows quick learning and data analysis. For example, a heat map view allows rapid screening and confirmation of samples while the instrument is acquiring data.

Autocleaning of autosampler is done during the instrument shutdown process therefore no additional user steps are required to maintain system.

Olympus™ 20X Objective, fluorite, 0.70NA/0.8–1.8WD, correction collar (0–1.6 mm) Invitrogen™

This fluorite (semi-apochromat) objective is equipped with an adjustable collar that affords flexibility in imaging through vessels with a thickness from 0 (no coverslip) to 2.0 mm. With four-color correction for spherical and chromatic aberrations, it delivers exceptional image quality. For the sharpest image simply set the collar to the thickness of the vessel/coverslip you are using. If the thickness is unknown, turn the collar in either direction while monitoring the sample focus. All Olympus objectives are compatible with EVOS cell imaging systems and offer outstanding optical performance from visible to near-infrared light. The extensive selection of EVOS and Olympus objectives satisfies needs across the spectrum of magnifications and optical specifications.

Additional characteristics of this Olympus objective:
• Magnification: 20X
• Numerical Aperture: 0.70
• Working Distance: 0.8–1.8 mm
• Superb image quality

Image quality
Microscope objectives may be the most important components of a microscope because they are responsible for primary image formation. High image quality is crucial to experimental success and often a requirement for publication; Olympus objectives afford that quality across the visible spectrum to near infrared light. This performance results from years of lens manufacture experience, and the broad selection of objectives means that you can choose the optimal objective for your imaging requirements.

Objective classes

Achromat objectives are perfect for general applications, with standard correction of color and focus.

Fluorite objectives deliver excellent resolution and are made with higher numerical apertures than achromat objectives, resulting in brighter fluorescence signal and higher contrast imaging. The higher optical quality greatly reduces optical aberrations, and corrections for color and focus are at higher levels than achromat objectives. Fluorite objectives are ideally suited for fluorescence and demanding transmitted light applications, where the higher contrast make them ideally suited for color imaging.

Apochromat objectives are manufactured to the highest levels of resolution, fluorescence brightness, and contrast; chromatic aberrations are almost eliminated. They are recommended for the most demanding applications, particularly at magnifications of 60x and above. Apochromatic objectives are the best choice for the capture of color images in white light.

Brightfield contrast versus phase contrast objectives
Brightfield is the most basic form of light microscopy and is accomplished by sample absorption of light. A higher density area in a sample will absorb more light, thus increasing contrast in those areas. Phase contrast objectives are most useful for hard to see, translucent specimens. This method of contrast is accomplished by converting phase shifts, caused by light passing through a translucent specimen, into brightness changes (i.e., contrast).

Long working distance versus coverslip-corrected objectives
Long working distance (LWD) objectives are optimized for use through vessels with a nominal wall thickness of 0.9-1.5 mm. This includes vessels commonly used in cell culture and cell-based assays, such as slides, cell culture dishes and flasks, microtiter well plates, etc. Coverslip-corrected objectives are optimized for use through #1.5 coverslips (thickness approximately 0.17 mm). These objectives have a higher magnification/NA ratio and provide higher resolution compared to LWD objectives.

For additional choices, visit the EVOS objectives selection guide.
Explore the entire EVOS line of imaging systems and accessories ›

4N6FLOQSwabs®, regular tip, tube Applied Biosystems™

4N6FLOQSwabs, regular tip, are comprised of a solid, molded plastic applicator shaft with a regular-sized tip. The tip of the applicator is coated with short Nylon fibers that are arranged in a perpendicular fashion. This perpendicular arrangement results from a process called flocking, where the fibers are sprayed onto the tip of the swab while it is held in an electrostatic field. This process creates a highly absorbent, thin layer with an open structure. The swab is supplied in a dry tube that helps prevent sample contamination during transport and is suitable for reference and evidence collection applications.

Key features:
• Certified free of DNase, RNase, and amplifiable human DNA
• Rapid absorption capability and superior sample release
• Regular-size tip for buccal cell collection
• Break point (from tip end): 20 mm

Unlike traditional fiber wound swabs, 4N6FLOQSwabs have no internal absorbent core to disperse and entrap the specimen—the sample stays close to the surface for faster and more efficient elution. The perpendicular Nylon fibers act like a soft brush that facilitates improved collection of cellular material. Capillary action between the fiber strands facilitates strong hydraulic uptake of liquid sample, and the sample stays close to the surface allowing easy elution.

4N6FLOQSwabs are manufactured by Copan, a market leader in the area of sample collection tools, and distributed globally by Thermo Fisher Scientific.

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